Restriction map for
pFN21AE3836
(Circular) (Six-base) MAPSORT of: pFN21AE3836.seq Check: 8054 from: 1 to: 18337
pFN21AE3836 18337 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2408 2489 4694 4817 4937 5672 6571 6743 9560
Size: 81 2205 123 120 735 899 172 2817
Cuts at: 9560 13511 13589 13804 14803 14855 15098 2408
Size: 3951 78 215 999 52 243 5647
Fragments arranged by size:
5647 3951 2817 2205 999 899 735 243
215 172 123 120 81 78 52
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 14295 16147 278
Size: 53 83 186 936 12759 1852 2468
Fragments arranged by size:
12759 2468 1852 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2589 2663 3090 3147 4299 6057 6477 11736
Size: 652 74 427 57 1152 1758 420 5259
Cuts at: 11736 15680 1937
Size: 3944 4594
Fragments arranged by size:
5259 4594 3944 1758 1152 652 427 420
74 57
Acc65I G'GTAC_C
Cuts at: 11475 15658 11475
Size: 4183 14154
AclI AA'CG_TT
Cuts at: 1563 16464 16837 1563
Size: 14901 373 3063
Fragments arranged by size:
14901 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2411 2692 3517 3815 7201 8589 8758 9607
Size: 779 281 825 298 3386 1388 169 849
Cuts at: 9607 10943 13895 14266 15226 16400 17784 18052 1632
Size: 1336 2952 371 960 1174 1384 268 1917
Fragments arranged by size:
3386 2952 1917 1388 1384 1336 1174 960
849 825 779 371 298 281 268 169
AfeI AGC'GCT
Cuts at: 4613 10376 10988 13262 4613
Size: 5763 612 2274 9688
Fragments arranged by size:
9688 5763 2274 612
AflII C'TTAA_G
Cuts at: 820 1017 11159 820
Size: 197 10142 7998
Fragments arranged by size:
10142 7998 197
AflIII A'CryG_T
Cuts at: 1203 2942 4865 8295 8475 10145 10359 10940 11151
Size: 1739 1923 3430 180 1670 214 581 211
Cuts at: 11151 13290 13917 17236 18213 1203
Size: 2139 627 3319 977 1327
Fragments arranged by size:
3430 3319 2139 1923 1739 1670 1327 977
627 581 214 211 180
AgeI A'CCGG_T
Cuts at: 4719 11109 17168 4719
Size: 6390 6059 5888
Fragments arranged by size:
6390 6059 5888
AhdI GACnn_n'nnGTC
Cuts at: 3553 6364 17066 3553
Size: 2811 10702 4824
Fragments arranged by size:
10702 4824 2811
AleI CACnn'nnGTG
Cuts at: 1166 2373 3368 4561 5457 6947 7490 8474 8837
Size: 1207 995 1193 896 1490 543 984 363
Cuts at: 8837 10358 10524 11946 11993 12953 14549 1166
Size: 1521 166 1422 47 960 1596 4954
Fragments arranged by size:
4954 1596 1521 1490 1422 1207 1193 995
984 960 896 543 363 166 47
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5252 5475 6465 10453 10789 11724 11964 12902
Size: 4068 223 990 3988 336 935 240 938
Cuts at: 12902 12971 14567 14835 15200 1184
Size: 69 1596 268 365 4321
Fragments arranged by size:
4321 4068 3988 1596 990 938 935 365
336 268 240 223 69
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5411 5443 5411
Size: 32 18305
AlwNI CAG_nnn'CTG
Cuts at: 4599 7105 8309 8509 9260 9478 9873 10258 10735
Size: 2506 1204 200 751 218 395 385 477
Cuts at: 10735 10857 11170 13377 13612 14847 15042 16080 17652
Size: 122 313 2207 235 1235 195 1038 1572
Cuts at: 17652 4599
Size: 5284
Fragments arranged by size:
5284 2506 2207 1572 1235 1204 1038 751
477 395 385 313 235 218 200 195
122
ApaI G_GGCC'C
Cuts at: 2322 2397 2656 3873 5652 6647 7235 14798 15222
Size: 75 259 1217 1779 995 588 7563 424
Cuts at: 15222 15608 2322
Size: 386 5051
Fragments arranged by size:
7563 5051 1779 1217 995 588 424 386
259 75
ApaLI G'TGCA_C
Cuts at: 6281 7621 16394 17550 6281
Size: 1340 8773 1156 7068
Fragments arranged by size:
8773 7068 1340 1156
ApoI r'AATT_y
Cuts at: 1493 7713 9565 11645 15646 15872 16024 18133 1493
Size: 6220 1852 2080 4001 226 152 2109 1697
Fragments arranged by size:
6220 4001 2109 2080 1852 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 16891 160
Size: 872 15859 1606
Fragments arranged by size:
15859 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 18337
AvaI C'yCGr_G
Cuts at: 1870 1943 3366 3417 3687 5090 6041 6192 7456
Size: 73 1423 51 270 1403 951 151 1264
Cuts at: 7456 8034 9094 10370 10556 11930 12350 12408 13064
Size: 578 1060 1276 186 1374 420 58 656
Cuts at: 13064 13435 13933 13993 15662 1870
Size: 371 498 60 1669 4545
Fragments arranged by size:
4545 1669 1423 1403 1374 1276 1264 1060
951 656 578 498 420 371 270 186
151 73 60 58 51
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 9118 9151 9118
Size: 33 18304
BamHI G'GATC_C
Cuts at: 3970 6187 6201 7723 15667 16034 3970
Size: 2217 14 1522 7944 367 6273
Fragments arranged by size:
7944 6273 2217 1522 367 14
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2477 3383 4137 4412 5599
Size: 325 217 627 690 906 754 275 1187
Cuts at: 5599 5881 6270 6835 7541 8468 8499 8741 8753
Size: 282 389 565 706 927 31 242 12
Cuts at: 8753 10028 10352 11475 11789 13143 13499 13769 14735
Size: 1275 324 1123 314 1354 356 270 966
Cuts at: 14735 14775 15658 17113 618
Size: 40 883 1455 1842
Fragments arranged by size:
1842 1455 1354 1275 1187 1123 966 927
906 883 754 706 690 627 565 389
356 325 324 314 282 275 270 242
217 40 31 12
BanII G_rGCy'C
Cuts at: 1391 2322 2337 2397 2656 3694 3873 5547 5652
Size: 931 15 60 259 1038 179 1674 105
Cuts at: 5652 5998 6321 6647 7235 7463 8855 10622 11445
Size: 346 323 326 588 228 1392 1767 823
Cuts at: 11445 11452 11776 12102 12462 12585 13203 14013 14495
Size: 7 324 326 360 123 618 810 482
Cuts at: 14495 14798 15222 15608 15656 1391
Size: 303 424 386 48 4072
Fragments arranged by size:
4072 1767 1674 1392 1038 931 823 810
618 588 482 424 386 360 346 326
326 324 323 303 259 228 179 123
105 60 48 15 7
BbeI G_GCGC'C
Cuts at: 4141 6274 10032 13147 4141
Size: 2133 3758 3115 9331
Fragments arranged by size:
9331 3758 3115 2133
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3317 7881 8437 8577 9713 11836 13574
Size: 971 1418 4564 556 140 1136 2123 1738
Cuts at: 13574 14332 15232 928
Size: 758 900 4033
Fragments arranged by size:
4564 4033 2123 1738 1418 1136 971 900
758 556 140
BbvCI CC'TCA_GC
Cuts at: 4494 5921 10761 11306 4494
Size: 1427 4840 545 11525
Fragments arranged by size:
11525 4840 1427 545
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3067 3101 4689 4723 5884
Size: 34 50 34 1046 34 1588 34 1161
Cuts at: 5884 5918 9146 9180 11533 11567 13055 13089 13276
Size: 34 3228 34 2353 34 1488 34 187
Cuts at: 13276 13310 13879 13913 16528 16562 1903
Size: 34 569 34 2615 34 3678
Fragments arranged by size:
3678 3228 2615 2353 1588 1488 1161 1046
569 187 50 34 34 34 34 34
34 34 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2531 8103 8648 16229 17439 2531
Size: 5572 545 7581 1210 3429
Fragments arranged by size:
7581 5572 3429 1210 545
BclI T'GATC_A
Cuts at: 1548 7411 1548
Size: 5863 12474
BfrBI ATG'CAT
Cuts at: 5229 5229
Size: 18337
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2799 3528 3609
Size: 107 122 71 631 605 1127 729 81
Cuts at: 3609 4111 4572 5634 6316 7230 7239 7362 9525
Size: 502 461 1062 682 914 9 123 2163
Cuts at: 9525 11523 11661 13437 14784 14892 16948 136
Size: 1998 138 1776 1347 108 2056 1525
Fragments arranged by size:
2163 2056 1998 1776 1525 1347 1127 1062
914 729 682 631 605 502 461 138
123 122 108 107 81 71 9
BglII A'GATC_T
Cuts at: 5964 18332 5964
Size: 12368 5969
BlpI GC'TnA_GC
Cuts at: 4115 11684 14489 15754 4115
Size: 7569 2805 1265 6698
Fragments arranged by size:
7569 6698 2805 1265
Bme1580I G_kGCm'C
Cuts at: 1790 2322 2397 2656 2782 3388 3873 5652 5886
Size: 532 75 259 126 606 485 1779 234
Cuts at: 5886 6285 6647 6840 7235 7436 7544 7625 8403
Size: 399 362 193 395 201 108 81 778
Cuts at: 8403 8554 8756 11874 11943 12654 13078 13091 13455
Size: 151 202 3118 69 711 424 13 364
Cuts at: 13455 13772 14562 14738 14780 14798 15203 15222 15608
Size: 317 790 176 42 18 405 19 386
Cuts at: 15608 16398 17554 1790
Size: 790 1156 2573
Fragments arranged by size:
3118 2573 1779 1156 790 790 778 711
606 532 485 424 405 399 395 386
364 362 317 259 234 202 201 193
176 151 126 108 81 75 69 42
19 18 13
BmgBI CAC'GTC
Cuts at: 1318 4866 5311 5950 6770 6904 7596 7866 7879
Size: 3548 445 639 820 134 692 270 13
Cuts at: 7879 10146 10323 10941 11869 12238 14410 15238 1318
Size: 2267 177 618 928 369 2172 828 4417
Fragments arranged by size:
4417 3548 2267 2172 928 828 820 692
639 618 445 369 270 177 134 13
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3160 4012 4156
Size: 416 53 83 399 24 1743 852 144
Cuts at: 4156 4555 5053 5316 6091 6229 7012 7748 7825
Size: 399 498 263 775 138 783 736 77
Cuts at: 7825 9322 10210 10578 11344 11503 11626 11758 12243
Size: 1497 888 368 766 159 123 132 485
Cuts at: 12243 13912 14071 14194 14490 14869 16068 17026 442
Size: 1669 159 123 296 379 1199 958 1753
Fragments arranged by size:
1753 1743 1669 1497 1199 958 888 852
783 775 766 736 498 485 416 399
399 379 368 296 263 159 159 144
138 132 123 123 83 77 53 24
BmtI G_CTAG'C
Cuts at: 1056 12015 1056
Size: 10959 7378
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5422 6268 6313 9919 10009 5422
Size: 846 45 3606 90 13750
Fragments arranged by size:
13750 3606 846 90 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3920 4061 5005 5260 6356 7127 7352 8267
Size: 2681 141 944 255 1096 771 225 915
Cuts at: 8267 8353 8806 9012 9521 9850 16997 1239
Size: 86 453 206 509 329 7147 2579
Fragments arranged by size:
7147 2681 2579 1096 944 915 771 509
453 329 255 225 206 141 86
Bpu10I CC'TnA_GC
Cuts at: 4224 4494 5921 6510 7832 8694 10761 11306 14393
Size: 270 1427 589 1322 862 2067 545 3087
Cuts at: 14393 15357 4224
Size: 964 7204
Fragments arranged by size:
7204 3087 2067 1427 1322 964 862 589
545 270
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2756 8615 10865 16462 17327 17625 2756
Size: 5859 2250 5597 865 298 3468
Fragments arranged by size:
5859 5597 3468 2250 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2717 3704 3779 6284 7012 8115 8966
Size: 617 1218 987 75 2505 728 1103 851
Cuts at: 8966 10178 12786 17000 882
Size: 1212 2608 4214 2219
Fragments arranged by size:
4214 2608 2505 2219 1218 1212 1103 987
851 728 617 75
BsaAI yAC'GTr
Cuts at: 493 1204 2640 2848 4549 8005 9349 10360 14769
Size: 711 1436 208 1701 3456 1344 1011 4409
Cuts at: 14769 16128 493
Size: 1359 2702
Fragments arranged by size:
4409 3456 2702 1701 1436 1359 1344 1011
711 208
BsaBI GATnn'nnATC
Cuts at: 2861 3162 6779 18331 2861
Size: 301 3617 11552 2867
Fragments arranged by size:
11552 3617 2867 301
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2070 3409 4138 4521
Size: 53 83 186 936 537 1339 729 383
Cuts at: 4521 6271 6360 8421 9189 9642 9900 10029 11625
Size: 1750 89 2061 768 453 258 129 1596
Cuts at: 11625 12909 13144 14193 14292 16144 16526 17161 275
Size: 1284 235 1049 99 1852 382 635 1451
Fragments arranged by size:
2061 1852 1750 1596 1451 1339 1284 1049
936 768 729 635 537 453 383 382
258 235 186 129 99 89 83 53
BsaWI w'CCGG_w
Cuts at: 4200 4719 6135 6678 7641 11109 16770 17168 17442
Size: 519 1416 543 963 3468 5661 398 274
Cuts at: 17442 17589 18000 4200
Size: 147 411 4537
Fragments arranged by size:
5661 4537 3468 1416 963 543 519 411
398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4034 4064 4279 4309 4811
Size: 30 165 30 2631 30 215 30 502
Cuts at: 4811 4841 6578 6608 6809 6839 6992 7022 8048
Size: 30 1737 30 201 30 153 30 1026
Cuts at: 8048 8078 8804 8834 8837 8867 9090 9120 9674
Size: 30 726 30 3 30 223 30 554
Cuts at: 9674 9704 13232 13262 1178
Size: 30 3528 30 6253
Fragments arranged by size:
6253 3528 2631 1737 1026 726 554 502
223 215 201 165 153 30 30 30
30 30 30 30 30 30 30 30
30 30 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3439 4022 5429 10324 11795 12950 13082 13261
Size: 2253 583 1407 4895 1471 1155 132 179
Cuts at: 13261 13264 13337 15245 1186
Size: 3 73 1908 4278
Fragments arranged by size:
4895 4278 2253 1908 1471 1407 1155 583
179 132 73 3
BseYI C'CCAG_C
Cuts at: 2211 2733 3303 4133 4395 4663 5644 5757 6266
Size: 522 570 830 262 268 981 113 509
Cuts at: 6266 6564 7360 7578 8883 9071 9387 10010 10014
Size: 298 796 218 1305 188 316 623 4
Cuts at: 10014 12529 13509 14712 14808 15119 17540 18036 2211
Size: 2515 980 1203 96 311 2421 496 2512
Fragments arranged by size:
2515 2512 2421 1305 1203 981 980 830
796 623 570 522 509 496 316 311
298 268 262 218 188 113 96 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2825 5140 7118 8696 9350 9742 10434
Size: 573 1089 2315 1978 1578 654 392 692
Cuts at: 10434 10554 10678 10759 10981 11303 11486 11804 11989
Size: 120 124 81 222 322 183 318 185
Cuts at: 11989 12145 12454 12697 12814 14144 14494 14833 14977
Size: 156 309 243 117 1330 350 339 144
Cuts at: 14977 15055 15082 1163
Size: 78 27 4418
Fragments arranged by size:
4418 2315 1978 1578 1330 1089 692 654
573 392 350 339 322 318 309 243
222 185 183 156 144 124 120 117
81 78 27
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4168 10465 10525 10936 11518 12427
Size: 275 186 2167 6297 60 411 582 909
Cuts at: 12427 12658 12760 14908 15787 16548 17576 1540
Size: 231 102 2148 879 761 1028 2301
Fragments arranged by size:
6297 2301 2167 2148 1028 909 879 761
582 411 275 231 186 102 60
BsiHKAI G_wGCw'C
Cuts at: 2337 2468 2684 2817 3240 3657 5097 5547 5561
Size: 131 216 133 423 417 1440 450 14
Cuts at: 5561 5744 6168 6285 7625 7838 8700 9297 9583
Size: 183 424 117 1340 213 862 597 286
Cuts at: 9583 9972 10622 10710 12149 12386 14721 14930 15237
Size: 389 650 88 1439 237 2335 209 307
Cuts at: 15237 16398 16483 17554 2337
Size: 1161 85 1071 3120
Fragments arranged by size:
3120 2335 1440 1439 1340 1161 1071 862
650 597 450 424 423 417 389 307
286 237 216 213 209 183 133 131
117 88 85 14
BsmI GAATG_Cn'
Cuts at: 13682 15848 15941 13682
Size: 2166 93 16078
Fragments arranged by size:
16078 2166 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4169 6967 7595 14078 4169
Size: 2798 628 6483 8428
Fragments arranged by size:
8428 6483 2798 628
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2322 2337 2397 2468 2656 2684 2782
Size: 399 532 15 60 71 188 28 98
Cuts at: 2782 2817 3240 3388 3657 3694 3873 5097 5547
Size: 35 423 148 269 37 179 1224 450
Cuts at: 5547 5561 5652 5744 5886 5998 6168 6285 6321
Size: 14 91 92 142 112 170 117 36
Cuts at: 6321 6647 6840 7235 7436 7463 7544 7625 7838
Size: 326 193 395 201 27 81 81 213
Cuts at: 7838 8403 8554 8700 8756 8855 9297 9583 9972
Size: 565 151 146 56 99 442 286 389
Cuts at: 9972 10622 10710 11445 11452 11776 11874 11943 12102
Size: 650 88 735 7 324 98 69 159
Cuts at: 12102 12149 12386 12462 12585 12654 13078 13091 13203
Size: 47 237 76 123 69 424 13 112
Cuts at: 13203 13455 13772 14013 14495 14562 14721 14738 14780
Size: 252 317 241 482 67 159 17 42
Cuts at: 14780 14798 14930 15203 15222 15237 15608 15656 16398
Size: 18 132 273 19 15 371 48 742
Cuts at: 16398 16483 17554 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1224 1071 742 735 650 565 532
482 450 442 424 423 399 395 389
371 326 324 317 286 273 269 252
241 237 213 201 193 188 179 170
159 159 151 148 146 142 132 123
117 112 112 99 98 98 92 91
88 85 81 81 76 71 69 69
67 60 56 48 47 42 37 36
35 28 27 19 18 17 15 15
14 13 7
BspEI T'CCGG_A
Cuts at: 6135 7641 18000 6135
Size: 1506 10359 6472
Fragments arranged by size:
10359 6472 1506
BspHI T'CATG_A
Cuts at: 3678 6708 13563 16226 17956 3678
Size: 3030 6855 2663 1730 4059
Fragments arranged by size:
6855 4059 3030 2663 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2408 2453 2489 3521 4694 4817 4937 4961
Size: 1564 45 36 1032 1173 123 120 24
Cuts at: 4961 5111 5672 6571 6585 6743 8613 9560 9641
Size: 150 561 899 14 158 1870 947 81
Cuts at: 9641 9860 10583 11127 11275 11844 12368 12971 13511
Size: 219 723 544 148 569 524 603 540
Cuts at: 13511 13589 13804 14616 14803 14855 15098 15140 15692
Size: 78 215 812 187 52 243 42 552
Cuts at: 15692 844
Size: 3489
Fragments arranged by size:
3489 1870 1564 1173 1032 947 899 812
723 603 569 561 552 544 540 524
243 219 215 187 158 150 148 123
120 81 78 52 45 42 36 24
14
BsrBI CCG'CTC
Cuts at: 1394 1823 2562 3781 4360 4896 7611 8107 9211
Size: 429 739 1219 579 536 2715 496 1104
Cuts at: 9211 9560 9988 12870 13434 14176 16224 1394
Size: 349 428 2882 564 742 2048 3507
Fragments arranged by size:
3507 2882 2715 2048 1219 1104 742 739
579 564 536 496 429 428 349
BsrDI GCAATG_nn'
Cuts at: 65 1248 2287 2951 2985 7911 9733 10723 11284
Size: 1183 1039 664 34 4926 1822 990 561
Cuts at: 11284 12310 14734 15157 16832 17006 65
Size: 1026 2424 423 1675 174 1396
Fragments arranged by size:
4926 2424 1822 1675 1396 1183 1039 1026
990 664 561 423 174 34
BsrFI r'CCGG_y
Cuts at: 1700 1810 2592 3603 3713 4719 5030 5211 6152
Size: 110 782 1011 110 1006 311 181 941
Cuts at: 6152 6293 6527 7628 8411 8457 8597 11109 11823
Size: 141 234 1101 783 46 140 2512 714
Cuts at: 11823 12126 13098 13827 14772 14919 16981 17168 1700
Size: 303 972 729 945 147 2062 187 2869
Fragments arranged by size:
2869 2512 2062 1101 1011 1006 972 945
941 783 782 729 714 311 303 234
187 181 147 141 140 110 110 46
BsrGI T'GTAC_A
Cuts at: 96 10443 13614 96
Size: 10347 3171 4819
Fragments arranged by size:
10347 4819 3171
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 18337
BssSI C'ACGA_G
Cuts at: 3587 5170 6428 16397 17409 3587
Size: 1583 1258 9969 1012 4515
Fragments arranged by size:
9969 4515 1583 1258 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2445 4455 10323 11916 14469 794
Size: 1651 2010 5868 1593 2553 4662
Fragments arranged by size:
5868 4662 2553 2010 1651 1593
BstBI TT'CG_AA
Cuts at: 17141 17141
Size: 18337
BstEII G'GTnAC_C
Cuts at: 1184 7882 11498 12355 1184
Size: 6698 3616 857 7166
Fragments arranged by size:
7166 6698 3616 857
BstXI CCAn_nnnn'nTGG
Cuts at: 2701 4241 5908 6148 7378 9148 11452 12537 13154
Size: 1540 1667 240 1230 1770 2304 1085 617
Cuts at: 13154 14026 14152 14357 15400 2701
Size: 872 126 205 1043 5638
Fragments arranged by size:
5638 2304 1770 1667 1540 1230 1085 1043
872 617 240 205 126
BstYI r'GATC_y
Cuts at: 1972 2171 2913 3036 3970 4377 5670 5964 6187
Size: 199 742 123 934 407 1293 294 223
Cuts at: 6187 6201 6780 7723 9795 13635 14346 15667 16034
Size: 14 579 943 2072 3840 711 1321 367
Cuts at: 16034 16419 16436 17888 17974 17986 18332 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
3840 2072 1977 1452 1321 1293 943 934
742 711 579 407 385 367 346 294
223 199 123 86 17 14 12
Bsu36I CC'TnA_GG
Cuts at: 15609 15609
Size: 18337
BtgI C'CryG_G
Cuts at: 513 1065 2700 3003 3828 4255 5971 6324 6543
Size: 552 1635 303 825 427 1716 353 219
Cuts at: 6543 6606 6985 7117 7236 7464 8527 8554 8856
Size: 63 379 132 119 228 1063 27 302
Cuts at: 8856 8878 9005 9396 9411 11002 11044 11829 14157
Size: 22 127 391 15 1591 42 785 2328
Cuts at: 14157 14781 15547 513
Size: 624 766 3303
Fragments arranged by size:
3303 2328 1716 1635 1591 1063 825 785
766 624 552 427 391 379 353 303
302 228 219 132 127 119 63 42
27 22 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2230 5121 5265 5295 5887 8226
Size: 683 56 1004 2891 144 30 592 2339
Cuts at: 8226 8698 9391 10220 10287 10411 11994 12198 12751
Size: 472 693 829 67 124 1583 204 553
Cuts at: 12751 12801 13038 13167 13603 13876 14108 487
Size: 50 237 129 436 273 232 4716
Fragments arranged by size:
4716 2891 2339 1583 1004 829 693 683
592 553 472 436 273 237 232 204
144 129 124 67 56 50 30
BtsI GCAGTG_nn'
Cuts at: 2323 3550 4300 4360 6459 7098 7561 7923 9333
Size: 1227 750 60 2099 639 463 362 1410
Cuts at: 9333 9649 9725 9928 10189 10259 10690 10750 10766
Size: 316 76 203 261 70 431 60 16
Cuts at: 10766 12457 12671 12874 13222 14618 14930 14994 15142
Size: 1691 214 203 348 1396 312 64 148
Cuts at: 15142 15853 16647 16667 2323
Size: 711 794 20 3993
Fragments arranged by size:
3993 2099 1691 1410 1396 1227 794 750
711 639 463 431 362 348 316 312
261 214 203 203 148 76 70 64
60 60 20 16
ClaI AT'CG_AT
Cuts at: 1554 2904 5850 7341 1554
Size: 1350 2946 1491 12550
Fragments arranged by size:
12550 2946 1491 1350
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3870 3905 4744 4779 4990 5025 9445
Size: 35 3306 35 839 35 211 35 4420
Cuts at: 9445 9480 10021 10056 10834 10869 11881 11916 15058
Size: 35 541 35 778 35 1012 35 3142
Cuts at: 15058 15093 529
Size: 35 3773
Fragments arranged by size:
4420 3773 3306 3142 1012 839 778 541
211 35 35 35 35 35 35 35
35 35
DraI TTT'AAA
Cuts at: 15641 15641
Size: 18337
DraIII CAC_nnn'GTG
Cuts at: 2848 3814 8938 10510 11680 12999 13450 2848
Size: 966 5124 1572 1170 1319 451 7735
Fragments arranged by size:
7735 5124 1572 1319 1170 966 451
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2945 4079 5873 8441 11840 12236 13700
Size: 507 1629 1134 1794 2568 3399 396 1464
Cuts at: 13700 13847 17344 809
Size: 147 3497 1802
Fragments arranged by size:
3497 3399 2568 1802 1794 1629 1464 1134
507 396 147
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2634 2859 3489
Size: 54 1425 50 275 18 804 225 630
Cuts at: 3489 3605 3754 3825 3891 4461 4840 6033 6114
Size: 116 149 71 66 570 379 1193 81
Cuts at: 6114 6150 6403 6529 7182 7222 7380 7648 7897
Size: 36 253 126 653 40 158 268 249
Cuts at: 7897 8487 9150 9414 10780 10933 11515 12655 12757
Size: 590 663 264 1366 153 582 1140 102
Cuts at: 12757 12871 13456 13534 13654 13738 13908 14154 14359
Size: 114 585 78 120 84 170 246 205
Cuts at: 14359 15106 15139 15315 15567 15784 16673 18302 8
Size: 747 33 176 252 217 889 1629 43
Fragments arranged by size:
1629 1425 1366 1193 1140 889 804 747
663 653 630 590 585 582 570 379
275 268 264 253 252 249 246 225
217 205 176 170 158 153 149 126
120 116 114 102 84 81 78 71
66 54 50 43 40 36 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 10933 11515 12655 12757 15784 1537
Size: 275 9121 582 1140 102 3027 4090
Fragments arranged by size:
9121 4090 3027 1140 582 275 102
EarI CTCTTCn'nnn_
Cuts at: 1353 4274 5282 7220 9977 12585 12699 13182 16267
Size: 2921 1008 1938 2757 2608 114 483 3085
Cuts at: 16267 1353
Size: 3423
Fragments arranged by size:
3423 3085 2921 2757 2608 1938 1008 483
114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 9938 15453 16924 17298 17444 387
Size: 1064 138 8349 5515 1471 374 146 1280
Fragments arranged by size:
8349 5515 1471 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2335 5545 10620 2335
Size: 3210 5075 10052
Fragments arranged by size:
10052 5075 3210
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2411 2692 3517 3815 3920 4061 5005
Size: 393 779 281 825 298 105 141 944
Cuts at: 5005 5260 6356 7127 7201 7352 8267 8353 8589
Size: 255 1096 771 74 151 915 86 236
Cuts at: 8589 8758 8806 9012 9521 9607 9850 10943 13895
Size: 169 48 206 509 86 243 1093 2952
Cuts at: 13895 14266 15226 16400 16997 17784 18052 1239
Size: 371 960 1174 597 787 268 1524
Fragments arranged by size:
2952 1524 1174 1096 1093 960 944 915
825 787 779 771 597 509 393 371
298 281 268 255 243 236 206 169
151 141 105 86 86 74 48
EcoNI CCTnn'n_nnAGG
Cuts at: 5607 11723 5607
Size: 6116 12221
EcoO109I rG'GnC_Cy
Cuts at: 2182 2318 2319 2652 3643 4012 4220 4660 5175
Size: 136 1 333 991 369 208 440 515
Cuts at: 5175 5648 5761 6046 6129 6643 6644 7102 7231
Size: 473 113 285 83 514 1 458 129
Cuts at: 7231 7232 7764 8689 8958 9475 9849 10876 11167
Size: 1 532 925 269 517 374 1027 291
Cuts at: 11167 11287 11403 13609 14389 14795 15054 15218 15219
Size: 120 116 2206 780 406 259 164 1
Cuts at: 15219 15604 15605 15625 2182
Size: 385 1 20 4894
Fragments arranged by size:
4894 2206 1027 991 925 780 532 517
515 514 473 458 440 406 385 374
369 333 291 285 269 259 208 164
136 129 120 116 113 83 20 1
1 1 1 1
EcoRI G'AATT_C
Cuts at: 7713 9565 15646 16024 7713
Size: 1852 6081 378 10026
Fragments arranged by size:
10026 6081 1852 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6946 9196 10906 6946
Size: 2250 1710 14377
Fragments arranged by size:
14377 2250 1710
FseI GG_CCGG'CC
Cuts at: 3607 3607
Size: 18337
FspI TGC'GCA
Cuts at: 4352 5894 11123 13403 16843 4352
Size: 1542 5229 2280 3440 5846
Fragments arranged by size:
5846 5229 3440 2280 1542
FspAI rTGC'GCAy
Cuts at: 13403 13403
Size: 18337
HaeII r_GCGC'y
Cuts at: 1678 4141 4615 5248 5934 6274 9092 9736 10032
Size: 2463 474 633 686 340 2818 644 296
Cuts at: 10032 10126 10378 10612 10753 10990 12778 12898 13147
Size: 94 252 234 141 237 1788 120 249
Cuts at: 13147 13264 17484 1678
Size: 117 4220 2531
Fragments arranged by size:
4220 2818 2531 2463 1788 686 644 633
474 340 296 252 249 237 234 141
120 117 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2450 2482 2546 2578 3539
Size: 32 135 32 878 32 64 32 961
Cuts at: 3539 3571 4267 4299 4855 4887 5214 5246 5543
Size: 32 696 32 556 32 327 32 297
Cuts at: 5543 5575 6350 6382 6422 6454 6992 7024 7403
Size: 32 775 32 40 32 538 32 379
Cuts at: 7403 7435 8564 8596 8804 8836 9090 9122 9662
Size: 32 1129 32 208 32 254 32 540
Cuts at: 9662 9694 16978 17010 17052 17084 1373
Size: 32 7284 32 42 32 2626
Fragments arranged by size:
7284 2626 1129 961 878 775 696 556
540 538 379 327 297 254 208 135
64 42 40 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3091 7561 8146 9508 11893 14377
Size: 988 281 1153 4470 585 1362 2385 2484
Cuts at: 14377 14857 15067 15681 15927 669
Size: 480 210 614 246 3079
Fragments arranged by size:
4470 3079 2484 2385 1362 1153 988 614
585 480 281 246 210
HindIII A'AGCT_T
Cuts at: 748 14198 748
Size: 13450 4887
HpaI GTT'AAC
Cuts at: 15927 15927
Size: 18337
KasI G'GCGC_C
Cuts at: 4137 6270 10028 13143 4137
Size: 2133 3758 3115 9331
Fragments arranged by size:
9331 3758 3115 2133
KpnI G_GTAC'C
Cuts at: 11479 15662 11479
Size: 4183 14154
MfeI C'AATT_G
Cuts at: 15936 15936
Size: 18337
MluI A'CGCG_T
Cuts at: 18213 18213
Size: 18337
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4488 5850 7401 8628 8824 9768 11169 12024
Size: 3183 1362 1551 1227 196 944 1401 855
Cuts at: 12024 12518 13842 14591 17451 17635 1305
Size: 494 1324 749 2860 184 2007
Fragments arranged by size:
3183 2860 2007 1551 1401 1362 1324 1227
944 855 749 494 196 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2636 2861 3491 3893 4463
Size: 54 1425 343 804 225 630 402 570
Cuts at: 4463 6035 6116 6405 7184 7224 7382 7650 8489
Size: 1572 81 289 779 40 158 268 839
Cuts at: 8489 9152 9416 14361 15569 10
Size: 663 264 4945 1208 2778
Fragments arranged by size:
4945 2778 1572 1425 1208 839 804 779
663 630 570 402 343 289 268 264
225 158 81 54 40
MslI CAynn'nnrTG
Cuts at: 518 1166 2373 2699 3368 3435 3629 4561 5196
Size: 648 1207 326 669 67 194 932 635
Cuts at: 5196 5457 6413 6947 7178 7490 7916 8177 8294
Size: 261 956 534 231 312 426 261 117
Cuts at: 8294 8474 8837 8984 9237 9410 10133 10358 10524
Size: 180 363 147 253 173 723 225 166
Cuts at: 10524 10827 11384 11525 11946 11993 12476 12953 14024
Size: 303 557 141 421 47 483 477 1071
Cuts at: 14024 14549 15398 16295 16654 16813 518
Size: 525 849 897 359 159 2042
Fragments arranged by size:
2042 1207 1071 956 932 897 849 723
669 648 635 557 534 525 483 477
426 421 363 359 326 312 303 261
261 253 231 225 194 180 173 166
159 147 141 117 67 47
MspA1I CmG'CkG
Cuts at: 1606 3519 4599 4737 4845 5076 5973 7308 7360
Size: 1913 1080 138 108 231 897 1335 52
Cuts at: 7360 7528 8160 8509 8536 9334 9432 9699 9837
Size: 168 632 349 27 798 98 267 138
Cuts at: 9837 9873 10014 10433 10596 10902 11046 11064 12279
Size: 36 141 419 163 306 144 18 1215
Cuts at: 12279 12300 12504 12522 12630 12645 12660 12762 12840
Size: 21 204 18 108 15 15 102 78
Cuts at: 12840 13009 13356 13548 14631 14667 14910 14991 15042
Size: 169 347 192 1083 36 243 81 51
Cuts at: 15042 15123 15753 16430 17578 17823 1606
Size: 81 630 677 1148 245 2120
Fragments arranged by size:
2120 1913 1335 1215 1148 1083 1080 897
798 677 632 630 419 349 347 306
267 245 243 231 204 192 169 168
163 144 141 138 138 108 108 102
98 81 81 78 52 51 36 36
27 21 18 18 15 15
NaeI GCC'GGC
Cuts at: 3605 5032 5213 7630 8599 12128 3605
Size: 1427 181 2417 969 3529 9814
Fragments arranged by size:
9814 3529 2417 1427 969 181
NarI GG'CG_CC
Cuts at: 4138 6271 10029 13144 4138
Size: 2133 3758 3115 9331
Fragments arranged by size:
9331 3758 3115 2133
NcoI C'CATG_G
Cuts at: 513 1065 2700 3003 4255 6606 7117 9396 11002
Size: 552 1635 303 1252 2351 511 2279 1606
Cuts at: 11002 11829 15547 513
Size: 827 3718 3303
Fragments arranged by size:
3718 3303 2351 2279 1635 1606 1252 827
552 511 303
NdeI CA'TA_TG
Cuts at: 387 2519 387
Size: 2132 16205
NgoMIV G'CCGG_C
Cuts at: 3603 5030 5211 7628 8597 12126 3603
Size: 1427 181 2417 969 3529 9814
Fragments arranged by size:
9814 3529 2417 1427 969 181
NheI G'CTAG_C
Cuts at: 1052 12011 1052
Size: 10959 7378
NotI GC'GGCC_GC
Cuts at: 15784 15784
Size: 18337
NsiI A_TGCA'T
Cuts at: 5231 5231
Size: 18337
NspI r_CATG'y
Cuts at: 1132 1229 2744 2788 2946 7440 7621 8299 9751
Size: 97 1515 44 158 4494 181 678 1452
Cuts at: 9751 11843 13294 14550 14764 14958 15695 17240 1132
Size: 2092 1451 1256 214 194 737 1545 2229
Fragments arranged by size:
4494 2229 2092 1545 1515 1452 1451 1256
737 678 214 194 181 158 97 44
PasI CC'CwG_GG
Cuts at: 1348 3954 4469 8017 8954 11239 11445 1348
Size: 2606 515 3548 937 2285 206 8240
Fragments arranged by size:
8240 3548 2606 2285 937 515 206
PciI A'CATG_T
Cuts at: 2942 8295 13290 17236 2942
Size: 5353 4995 3946 4043
Fragments arranged by size:
5353 4995 4043 3946
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2868 3814 3980 4507 4737 4860 6522 6607
Size: 1760 946 166 527 230 123 1662 85
Cuts at: 6607 6991 7815 8938 9886 10140 10254 13902 14052
Size: 384 824 1123 948 254 114 3648 150
Cuts at: 14052 15565 1108
Size: 1513 3880
Fragments arranged by size:
3880 3648 1760 1662 1513 1123 948 946
824 527 384 254 230 166 150 123
114 85
PfoI T'CCnGG_A
Cuts at: 2289 3645 4605 4859 5763 7286 7760 8822 9537
Size: 1356 960 254 904 1523 474 1062 715
Cuts at: 9537 2289
Size: 11089
Fragments arranged by size:
11089 1523 1356 1062 960 904 715 474
254
PmeI GTTT'AAAC
Cuts at: 15641 15641
Size: 18337
PmlI CAC'GTG
Cuts at: 2848 9349 10360 14769 2848
Size: 6501 1011 4409 6416
Fragments arranged by size:
6501 6416 4409 1011
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 12725 12757 16406 16438 17919 17951 12725
Size: 32 3649 32 1481 32 13111
Fragments arranged by size:
13111 3649 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3643 4012 4220 5761 6129 7764 8689 9475 9849
Size: 369 208 1541 368 1635 925 786 374
Cuts at: 9849 10876 11287 13609 15625 3643
Size: 1027 411 2322 2016 6355
Fragments arranged by size:
6355 2322 2016 1635 1541 1027 925 786
411 374 369 368 208
PshAI GACnn'nnGTC
Cuts at: 1613 5309 6959 9480 15134 1613
Size: 3696 1650 2521 5654 4816
Fragments arranged by size:
5654 4816 3696 2521 1650
PsiI TTA'TAA
Cuts at: 15907 15907
Size: 18337
PspOMI G'GGCC_C
Cuts at: 2318 2393 2652 3869 5648 6643 7231 14794 15218
Size: 75 259 1217 1779 995 588 7563 424
Cuts at: 15218 15604 2318
Size: 386 5051
Fragments arranged by size:
7563 5051 1779 1217 995 588 424 386
259 75
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4529 4561 4529
Size: 32 18305
PstI C_TGCA'G
Cuts at: 830 2108 2486 2550 4448 5039 5441 5582 6582
Size: 1278 378 64 1898 591 402 141 1000
Cuts at: 6582 6970 7556 9923 9938 10862 10982 11909 13334
Size: 388 586 2367 15 924 120 927 1425
Cuts at: 13334 13547 14613 15149 15689 830
Size: 213 1066 536 540 3478
Fragments arranged by size:
3478 2367 1898 1425 1278 1066 1000 927
924 591 586 540 536 402 388 378
213 141 120 64 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 18337
PvuII CAG'CTG
Cuts at: 7360 7528 8160 8509 9432 10014 10433 11064 13356
Size: 168 632 349 923 582 419 631 2292
Cuts at: 13356 13548 14631 15123 7360
Size: 192 1083 492 10574
Fragments arranged by size:
10574 2292 1083 923 632 631 582 492
419 349 192 168
RsrII CG'GwC_CG
Cuts at: 4696 6681 4696
Size: 1985 16352
SacI G_AGCT'C
Cuts at: 2337 5547 10622 2337
Size: 3210 5075 10052
Fragments arranged by size:
10052 5075 3210
SacII CC_GC'GG
Cuts at: 5974 11047 5974
Size: 5073 13264
SalI G'TCGA_C
Cuts at: 1936 3089 15679 1936
Size: 1153 12590 4594
Fragments arranged by size:
12590 4594 1153
SanDI GG'GwC_CC
Cuts at: 6129 15625 6129
Size: 9496 8841
SapI GCTCTTCn'nnn_
Cuts at: 5282 7220 9977 5282
Size: 1938 2757 13642
Fragments arranged by size:
13642 2757 1938
SbfI CC_TGCA'GG
Cuts at: 5441 6582 15689 5441
Size: 1141 9107 8089
Fragments arranged by size:
9107 8089 1141
ScaI AGT'ACT
Cuts at: 16585 18297 16585
Size: 1712 16625
SexAI A'CCwGG_T
Cuts at: 3129 3354 4500 13605 3129
Size: 225 1146 9105 7861
Fragments arranged by size:
9105 7861 1146 225
SfcI C'TryA_G
Cuts at: 826 1045 2104 2482 2546 4444 5035 5131 5437
Size: 219 1059 378 64 1898 591 96 306
Cuts at: 5437 5578 5869 6054 6578 6966 7552 7633 9919
Size: 141 291 185 524 388 586 81 2286
Cuts at: 9919 9934 10048 10858 10978 11905 13330 13543 14609
Size: 15 114 810 120 927 1425 213 1066
Cuts at: 14609 15145 15685 16820 17501 17692 826
Size: 536 540 1135 681 191 1471
Fragments arranged by size:
2286 1898 1471 1425 1135 1066 1059 927
810 681 591 586 540 536 524 388
378 306 291 219 213 191 185 141
120 114 96 81 64 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 7230 7239 7230
Size: 9 18328
SfoI GGC'GCC
Cuts at: 4139 6272 10030 13145 4139
Size: 2133 3758 3115 9331
Fragments arranged by size:
9331 3758 3115 2133
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 18337
SgrAI Cr'CCGG_yG
Cuts at: 1700 13827 1700
Size: 12127 6210
SmaI CCC'GGG
Cuts at: 1872 3368 6194 8036 11932 12410 15664 1872
Size: 1496 2826 1842 3896 478 3254 4545
Fragments arranged by size:
4545 3896 3254 2826 1842 1496 478
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2771 8630 10880 11159 16441 17342
Size: 197 926 828 5859 2250 279 5282 901
Cuts at: 17342 17604 820
Size: 262 1553
Fragments arranged by size:
5859 5282 2250 1553 926 901 828 279
262 197
SnaBI TAC'GTA
Cuts at: 493 16128 493
Size: 15635 2702
SpeI A'CTAG_T
Cuts at: 152 6002 152
Size: 5850 12487
SphI G_CATG'C
Cuts at: 1132 2788 14764 15695 1132
Size: 1656 11976 931 3774
Fragments arranged by size:
11976 3774 1656 931
SspI AAT'ATT
Cuts at: 5 52 16261 5
Size: 47 16209 2081
Fragments arranged by size:
16209 2081 47
StuI AGG'CCT
Cuts at: 1517 7658 8865 11304 12941 14151 14268 15462 1517
Size: 6141 1207 2439 1637 1210 117 1194 4392
Fragments arranged by size:
6141 4392 2439 1637 1210 1207 1194 117
StyI C'CwwG_G
Cuts at: 513 1065 2338 2700 2979 3003 3639 4017 4255
Size: 552 1273 362 279 24 636 378 238
Cuts at: 4255 6170 6606 7117 9396 11002 11187 11829 12585
Size: 1915 436 511 2279 1606 185 642 756
Cuts at: 12585 13527 14370 15349 15547 15630 15776 513
Size: 942 843 979 198 83 146 3074
Fragments arranged by size:
3074 2279 1915 1606 1273 979 942 843
756 642 636 552 511 436 378 362
279 238 198 185 146 83 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2725 3972 4138 4713 5072 5362 5960
Size: 1255 1145 1247 166 575 359 290 598
Cuts at: 5960 6073 6183 6578 7175 7807 9018 9304 10006
Size: 113 110 395 597 632 1211 286 702
Cuts at: 10006 10024 10370 11015 11252 11326 11330 11759 12488
Size: 18 346 645 237 74 4 429 729
Cuts at: 12488 13235 13868 13894 13943 14822 16364 16381 16534
Size: 747 633 26 49 879 1542 17 153
Cuts at: 16534 16719 17164 325
Size: 185 445 1498
Fragments arranged by size:
1542 1498 1255 1247 1211 1145 879 747
729 702 645 633 632 598 597 575
445 429 395 359 346 290 286 237
185 166 153 113 110 74 49 26
18 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3852 3990 6891
Size: 274 80 33 51 1443 1875 138 2901
Cuts at: 6891 7686 8202 10443 11352 11481 13614 13920 14049
Size: 795 516 2241 909 129 2133 306 129
Cuts at: 14049 14181 16583 18295 96
Size: 132 2402 1712 138
Fragments arranged by size:
2901 2402 2241 2133 1875 1712 1443 909
795 516 306 274 138 138 132 129
129 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3448 3480 4034 4066 4279 4311 7150 7182 7385
Size: 32 554 32 213 32 2839 32 203
Cuts at: 7385 7417 7850 7882 13232 13264 18297 18329 3448
Size: 32 433 32 5350 32 5033 32 3456
Fragments arranged by size:
5350 5033 3456 2839 554 433 213 203
32 32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3131 4520 6685 6851 7004 9641 11597 11732
Size: 1815 1389 2165 166 153 2637 1956 135
Cuts at: 11732 13124 14411 1316
Size: 1392 1287 5242
Fragments arranged by size:
5242 2637 2165 1956 1815 1392 1389 1287
166 153 135
XbaI T'CTAG_A
Cuts at: 8023 15673 8023
Size: 7650 10687
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3767 3961 3981 7139 7816 8287 9346 10790 11452
Size: 194 20 3158 677 471 1059 1444 662
Cuts at: 11452 11968 12482 13733 13903 15149 15535 3767
Size: 516 514 1251 170 1246 386 6569
Fragments arranged by size:
6569 3158 1444 1251 1246 1059 677 662
516 514 471 386 194 170 20
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 18337
XmaI C'CCGG_G
Cuts at: 1870 3366 6192 8034 11930 12408 15662 1870
Size: 1496 2826 1842 3896 478 3254 4545
Fragments arranged by size:
4545 3896 3254 2826 1842 1496 478
XmnI GAAnn'nnTTC
Cuts at: 5726 9710 10103 11091 16466 5726
Size: 3984 393 988 5375 7597
Fragments arranged by size:
7597 5375 3984 988 393
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 14293 16145 276
Size: 53 83 186 936 12759 1852 2468
Fragments arranged by size:
12759 2468 1852 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI AvrII BsiWI BstZ17I EcoRV NruI PacI PspXI
SrfI SwaI