Restriction map for
pFN21AE3830
(Circular) (Six-base) MAPSORT of: pFN21AE3830.seq Check: 8346 from: 1 to: 13597
pFN21AE3830 13597 bp
With 182 enzymes: SgfI *
March 28, 2012 12:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2765 2765
Size: 13597
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 11407 278
Size: 53 83 186 936 9871 2468
Fragments arranged by size:
9871 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2329 7558 8057 8232 8706 9604 9942 10940
Size: 392 5229 499 175 474 898 338 998
Cuts at: 10940 1937
Size: 4594
Fragments arranged by size:
5229 4594 998 898 499 474 392 338
175
Acc65I G'GTAC_C
Cuts at: 10918 10918
Size: 13597
AclI AA'CG_TT
Cuts at: 1563 3840 4236 11724 12097 1563
Size: 2277 396 7488 373 3063
Fragments arranged by size:
7488 3063 2277 396 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2502 3990 4814 6672 7562 8975 11660 13044
Size: 870 1488 824 1858 890 1413 2685 1384
Cuts at: 13044 13312 1632
Size: 268 1917
Fragments arranged by size:
2685 1917 1858 1488 1413 1384 890 870
824 268
AfeI AGC'GCT
Cuts at: 6551 10526 6551
Size: 3975 9622
AflII C'TTAA_G
Cuts at: 820 1017 3311 820
Size: 197 2294 11106
Fragments arranged by size:
11106 2294 197
AflIII A'CryG_T
Cuts at: 1203 6615 12496 13473 1203
Size: 5412 5881 977 1327
Fragments arranged by size:
5881 5412 1327 977
AgeI A'CCGG_T
Cuts at: 12428 12428
Size: 13597
AhdI GACnn_n'nnGTC
Cuts at: 4462 12326 4462
Size: 7864 5733
AleI CACnn'nnGTG
Cuts at: 1166 5526 9141 1166
Size: 4360 3615 5622
Fragments arranged by size:
5622 4360 3615
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 10868 1184
Size: 9684 3913
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6657 6689 6657
Size: 32 13565
AlwNI CAG_nnn'CTG
Cuts at: 4067 9707 11340 12912 4067
Size: 5640 1633 1572 4752
Fragments arranged by size:
5640 4752 1633 1572
ApaLI G'TGCA_C
Cuts at: 11654 12810 11654
Size: 1156 12441
ApoI r'AATT_y
Cuts at: 1493 2254 3786 5509 5651 5904 8217 9342 10906
Size: 761 1532 1723 142 253 2313 1125 1564
Cuts at: 10906 11132 11284 13393 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2313 2109 1723 1697 1564 1532 1125 761
253 226 152 142
AseI AT'TA_AT
Cuts at: 160 1032 2571 2955 3468 4937 12151 160
Size: 872 1539 384 513 1469 7214 1606
Fragments arranged by size:
7214 1606 1539 1469 872 513 384
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13597
AvaI C'yCGr_G
Cuts at: 1870 1943 5940 9309 10922 1870
Size: 73 3997 3369 1613 4545
Fragments arranged by size:
4545 3997 3369 1613 73
AvrII C'CTAG_G
Cuts at: 8512 8512
Size: 13597
BamHI G'GATC_C
Cuts at: 2605 4834 5854 10927 11294 2605
Size: 2229 1020 5073 367 4908
Fragments arranged by size:
5073 4908 2229 1020 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6456 10219 10918 12373 618
Size: 325 217 627 4669 3763 699 1455 1842
Fragments arranged by size:
4669 3763 1842 1455 699 627 325 217
BanII G_rGCy'C
Cuts at: 1391 5363 7191 8481 10916 1391
Size: 3972 1828 1290 2435 4072
Fragments arranged by size:
4072 3972 2435 1828 1290
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2489 2691 4979 5328 7056 9214 928
Size: 971 590 202 2288 349 1728 2158 5311
Fragments arranged by size:
5311 2288 2158 1728 971 590 349 202
BbvCI CC'TCA_GC
Cuts at: 5783 8445 5783
Size: 2662 10935
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6008 6042 9236 9270 11788 11822 1903
Size: 34 4071 34 3194 34 2518 34 3678
Fragments arranged by size:
4071 3678 3194 2518 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6225 8394 11489 12699 6225
Size: 2169 3095 1210 7123
Fragments arranged by size:
7123 3095 2169 1210
BclI T'GATC_A
Cuts at: 1548 2763 3252 10423 1548
Size: 1215 489 7171 4722
Fragments arranged by size:
7171 4722 1215 489
BfrBI ATG'CAT
Cuts at: 3504 6286 3504
Size: 2782 10815
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4687 8376 12208
Size: 107 122 71 631 605 3015 3689 3832
Cuts at: 12208 136
Size: 1525
Fragments arranged by size:
3832 3689 3015 1525 631 605 122 107
71
BglII A'GATC_T
Cuts at: 5944 9330 9447 9463 10767 13592 5944
Size: 3386 117 16 1304 2825 5949
Fragments arranged by size:
5949 3386 2825 1304 117 16
BlpI GC'TnA_GC
Cuts at: 3005 5726 11014 3005
Size: 2721 5288 5588
Fragments arranged by size:
5588 5288 2721
Bme1580I G_kGCm'C
Cuts at: 1790 11658 12814 1790
Size: 9868 1156 2573
Fragments arranged by size:
9868 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 7952 1318
Size: 6634 6963
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2936 4064 5388
Size: 416 53 83 399 24 1519 1128 1324
Cuts at: 5388 5592 6399 7854 8982 9109 9226 9575 10705
Size: 204 807 1455 1128 127 117 349 1130
Cuts at: 10705 11328 12286 442
Size: 623 958 1753
Fragments arranged by size:
1753 1519 1455 1324 1130 1128 1128 958
807 623 416 399 349 204 127 117
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 13597
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8990 9466 8990
Size: 476 13121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2261 2526 4420 5407 5897 6850 8046 8072
Size: 1022 265 1894 987 490 953 1196 26
Cuts at: 8072 10652 10850 12257 1239
Size: 2580 198 1407 2579
Fragments arranged by size:
2580 2579 1894 1407 1196 1022 987 953
490 265 198 26
Bpu10I CC'TnA_GC
Cuts at: 5783 8445 5783
Size: 2662 10935
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4910 5243 5545 11722 12587 12885 4910
Size: 333 302 6177 865 298 5622
Fragments arranged by size:
6177 5622 865 333 302 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2418 4197 5582 6052 9689 9785 10294
Size: 617 919 1779 1385 470 3637 96 509
Cuts at: 10294 12260 882
Size: 1966 2219
Fragments arranged by size:
3637 2219 1966 1779 1385 919 617 509
470 96
BsaAI yAC'GTr
Cuts at: 493 1204 10287 11388 493
Size: 711 9083 1101 2702
Fragments arranged by size:
9083 2702 1101 711
BsaBI GATnn'nnATC
Cuts at: 13591 13591
Size: 13597
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 11404 11786 12421 275
Size: 53 83 186 936 9871 382 635 1451
Fragments arranged by size:
9871 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3592 8709 10383 12030 12428 12702 12849 13260 3592
Size: 5117 1674 1647 398 274 147 411 3929
Fragments arranged by size:
5117 3929 1674 1647 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2671 2701 3985 4015 6169
Size: 30 165 30 1268 30 1284 30 2154
Cuts at: 6169 6199 6848 6878 1178
Size: 30 649 30 7897
Fragments arranged by size:
7897 2154 1284 1268 649 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 5481 5491 5686 6228 10649 1186
Size: 4295 10 195 542 4421 4134
Fragments arranged by size:
4421 4295 4134 542 195 10
BseYI C'CCAG_C
Cuts at: 2183 5246 5835 6993 7017 7876 8489 8744 10625
Size: 3063 589 1158 24 859 613 255 1881
Cuts at: 10625 12800 13296 2183
Size: 2175 496 2484
Fragments arranged by size:
3063 2484 2175 1881 1158 859 613 589
496 255 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3669 4080 6894 7215 7646 10387 1163
Size: 573 1933 411 2814 321 431 2741 4373
Fragments arranged by size:
4373 2814 2741 1933 573 431 411 321
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 11047 11808 12836 1540
Size: 275 186 9046 761 1028 2301
Fragments arranged by size:
9046 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 6447 7037 9855 11658 11743 12814 6447
Size: 590 2818 1803 85 1071 7230
Fragments arranged by size:
7230 2818 1803 1071 590 85
BsmI GAATG_Cn'
Cuts at: 2477 3504 3987 5677 7601 9433 9556 11108 11201
Size: 1027 483 1690 1924 1832 123 1552 93
Cuts at: 11201 2477
Size: 4873
Fragments arranged by size:
4873 1924 1832 1690 1552 1027 483 123
93
BsmBI CGTCTCn'nnnn_
Cuts at: 7635 7635
Size: 13597
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 5363 6447 7037 7191 8481 9855 10916
Size: 399 3573 1084 590 154 1290 1374 1061
Cuts at: 10916 11658 11743 12814 1391
Size: 742 85 1071 2174
Fragments arranged by size:
3573 2174 1374 1290 1084 1071 1061 742
590 399 154 85
BspEI T'CCGG_A
Cuts at: 3592 13260 3592
Size: 9668 3929
BspHI T'CATG_A
Cuts at: 4318 5773 9225 9783 11486 13216 4318
Size: 1455 3452 558 1703 1730 4699
Fragments arranged by size:
4699 3452 1730 1703 1455 558
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2341 2765 3236 5369 10271 10952 844
Size: 1497 424 471 2133 4902 681 3489
Fragments arranged by size:
4902 3489 2133 1497 681 471 424
BsrBI CCG'CTC
Cuts at: 1394 1823 11484 1394
Size: 429 9661 3507
Fragments arranged by size:
9661 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3198 5460 6087 8390 8429 12092 12266
Size: 1183 1950 2262 627 2303 39 3663 174
Cuts at: 12266 65
Size: 1396
Fragments arranged by size:
3663 2303 2262 1950 1396 1183 627 174
39
BsrFI r'CCGG_y
Cuts at: 1700 1810 4188 12241 12428 1700
Size: 110 2378 8053 187 2869
Fragments arranged by size:
8053 2869 2378 187 110
BsrGI T'GTAC_A
Cuts at: 96 2439 10329 96
Size: 2343 7890 3364
Fragments arranged by size:
7890 3364 2343
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 13597
BssSI C'ACGA_G
Cuts at: 11657 12669 11657
Size: 1012 12585
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4067 7915 794
Size: 3273 3848 6476
Fragments arranged by size:
6476 3848 3273
BstBI TT'CG_AA
Cuts at: 5842 5965 6028 9265 10891 12401 5842
Size: 123 63 3237 1626 1510 7038
Fragments arranged by size:
7038 3237 1626 1510 123 63
BstEII G'GTnAC_C
Cuts at: 1184 4698 10145 1184
Size: 3514 5447 4636
Fragments arranged by size:
5447 4636 3514
BstYI r'GATC_y
Cuts at: 1972 2605 2860 3539 4834 5854 5944 9186 9330
Size: 633 255 679 1295 1020 90 3242 144
Cuts at: 9330 9447 9463 9486 10377 10767 10927 11294 11679
Size: 117 16 23 891 390 160 367 385
Cuts at: 11679 11696 13148 13234 13246 13592 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
3242 1977 1452 1295 1020 891 679 633
390 385 367 346 255 160 144 117
90 86 23 17 16 12
BstZ17I GTA'TAC
Cuts at: 8058 8707 8058
Size: 649 12948
Bsu36I CC'TnA_GG
Cuts at: 10728 10728
Size: 13597
BtgI C'CryG_G
Cuts at: 513 1065 2004 7191 8895 513
Size: 552 939 5187 1704 5215
Fragments arranged by size:
5215 5187 1704 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 12858
Fragments arranged by size:
12858 683 56
BtsI GCAGTG_nn'
Cuts at: 3287 4081 4807 6808 6950 7658 8171 9697 9875
Size: 794 726 2001 142 708 513 1526 178
Cuts at: 9875 10656 11113 11907 11927 3287
Size: 781 457 794 20 4957
Fragments arranged by size:
4957 2001 1526 794 794 781 726 708
513 457 178 142 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 13597
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 9867 9902 529
Size: 35 9303 35 4224
Fragments arranged by size:
9303 4224 35 35
DraI TTT'AAA
Cuts at: 2259 2616 3601 4050 4231 4545 4980 10901 2259
Size: 357 985 449 181 314 435 5921 4955
Fragments arranged by size:
5921 4955 985 449 435 357 314 181
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 12604 809
Size: 507 11288 1802
Fragments arranged by size:
11288 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3153 6871 7022
Size: 54 1425 50 275 18 1323 3718 151
Cuts at: 7022 7455 8898 10030 11044 11933 13562 8
Size: 433 1443 1132 1014 889 1629 43
Fragments arranged by size:
3718 1629 1443 1425 1323 1132 1014 889
433 275 151 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 11044 1537
Size: 275 9232 4090
Fragments arranged by size:
9232 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2136 2595 3952 5149 6526 7742 7806 8927
Size: 783 459 1357 1197 1377 1216 64 1121
Cuts at: 8927 10578 11527 1353
Size: 1651 949 3423
Fragments arranged by size:
3423 1651 1377 1357 1216 1197 1121 949
783 459 64
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 12184 12558 12704 387
Size: 1064 138 10595 374 146 1280
Fragments arranged by size:
10595 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2261 2502 2526 3990 4420 4814 5407
Size: 393 629 241 24 1464 430 394 593
Cuts at: 5407 5897 6672 6850 7562 8046 8072 8975 10652
Size: 490 775 178 712 484 26 903 1677
Cuts at: 10652 10850 11660 12257 13044 13312 1239
Size: 198 810 597 787 268 1524
Fragments arranged by size:
1677 1524 1464 903 810 787 775 712
629 597 593 490 484 430 394 393
268 241 198 178 26 24
EcoNI CCTnn'n_nnAGG
Cuts at: 3243 8516 9767 3243
Size: 5273 1251 7073
Fragments arranged by size:
7073 5273 1251
EcoO109I rG'GnC_Cy
Cuts at: 2146 10260 2146
Size: 8114 5483
EcoRI G'AATT_C
Cuts at: 10906 11284 10906
Size: 378 13219
EcoRV GAT'ATC
Cuts at: 8155 8155
Size: 13597
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2165 3990 7341 8833 10881 2165
Size: 1825 3351 1492 2048 4881
Fragments arranged by size:
4881 3351 2048 1825 1492
FspI TGC'GCA
Cuts at: 12103 12103
Size: 13597
HaeII r_GCGC'y
Cuts at: 1678 6553 8096 10528 12744 1678
Size: 4875 1543 2432 2216 2531
Fragments arranged by size:
4875 2531 2432 2216 1543
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3506 3538 4448 4480 4807
Size: 32 135 32 1934 32 910 32 327
Cuts at: 4807 4839 5143 5175 5431 5463 5902 5934 6503
Size: 32 304 32 256 32 439 32 569
Cuts at: 6503 6535 6998 7030 7702 7734 7846 7878 8823
Size: 32 463 32 672 32 112 32 945
Cuts at: 8823 8855 8956 8988 9439 9471 9997 10029 10823
Size: 32 101 32 451 32 526 32 794
Cuts at: 10823 10855 12238 12270 12312 12344 1373
Size: 32 1383 32 42 32 2626
Fragments arranged by size:
2626 1934 1383 945 910 794 672 569
526 463 451 439 327 304 256 135
112 101 42 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2330 4015 7362 7559 7624 10716
Size: 988 281 392 1685 3347 197 65 3092
Cuts at: 10716 10941 11187 669
Size: 225 246 3079
Fragments arranged by size:
3347 3092 3079 1685 988 392 281 246
225 197 65
HindIII A'AGCT_T
Cuts at: 748 6646 6757 7831 9516 10106 748
Size: 5898 111 1074 1685 590 4239
Fragments arranged by size:
5898 4239 1685 1074 590 111
HpaI GTT'AAC
Cuts at: 4015 11187 4015
Size: 7172 6425
KpnI G_GTAC'C
Cuts at: 10922 10922
Size: 13597
MfeI C'AATT_G
Cuts at: 11196 11196
Size: 13597
MluI A'CGCG_T
Cuts at: 13473 13473
Size: 13597
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2824 3079 3781 6413 6556 8086 9158 10254
Size: 1519 255 702 2632 143 1530 1072 1096
Cuts at: 10254 12711 12895 1305
Size: 2457 184 2007
Fragments arranged by size:
2632 2457 2007 1530 1519 1096 1072 702
255 184 143
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3155 7457 8900 10032 10
Size: 54 1425 343 1323 4302 1443 1132 3575
Fragments arranged by size:
4302 3575 1443 1425 1323 1132 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5526 5583 8076 8661 9141 10814 11555
Size: 648 4360 57 2493 585 480 1673 741
Cuts at: 11555 11914 12073 518
Size: 359 159 2042
Fragments arranged by size:
4360 2493 2042 1673 741 648 585 480
359 159 57
MspA1I CmG'CkG
Cuts at: 1606 2671 2945 5246 6182 6843 7021 7162 7371
Size: 1065 274 2301 936 661 178 141 209
Cuts at: 7371 8744 9284 11013 11690 12838 13083 1606
Size: 1373 540 1729 677 1148 245 2120
Fragments arranged by size:
2301 2120 1729 1373 1148 1065 936 677
661 540 274 245 209 178 141
NcoI C'CATG_G
Cuts at: 513 1065 2004 7191 8895 513
Size: 552 939 5187 1704 5215
Fragments arranged by size:
5215 5187 1704 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 13597
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 13597
NotI GC'GGCC_GC
Cuts at: 11044 11044
Size: 13597
NsiI A_TGCA'T
Cuts at: 3506 6288 3506
Size: 2782 10815
NspI r_CATG'y
Cuts at: 1132 1229 3504 4188 5693 6290 6619 8740 9535
Size: 97 2275 684 1505 597 329 2121 795
Cuts at: 9535 10955 12500 1132
Size: 1420 1545 2229
Fragments arranged by size:
2275 2229 2121 1545 1505 1420 795 684
597 329 97
PasI CC'CwG_GG
Cuts at: 1348 7279 1348
Size: 5931 7666
PciI A'CATG_T
Cuts at: 6615 12496 6615
Size: 5881 7716
PflMI CCAn_nnn'nTGG
Cuts at: 1108 6149 8896 1108
Size: 5041 2747 5809
Fragments arranged by size:
5809 5041 2747
PfoI T'CCnGG_A
Cuts at: 6534 8062 10511 6534
Size: 1528 2449 9620
Fragments arranged by size:
9620 2449 1528
PmeI GTTT'AAAC
Cuts at: 10901 10901
Size: 13597
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 11666 11698 13179 13211 11666
Size: 32 1481 32 12052
Fragments arranged by size:
12052 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2146 10260 2146
Size: 8114 5483
PshAI GACnn'nnGTC
Cuts at: 1613 9701 1613
Size: 8088 5509
PsiI TTA'TAA
Cuts at: 4476 8769 9366 11167 4476
Size: 4293 597 1801 6906
Fragments arranged by size:
6906 4293 1801 597
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2719 2751 2923 2955 2974 3006 7083 7115 2719
Size: 32 172 32 19 32 4077 32 9201
Fragments arranged by size:
9201 4077 172 32 32 32 32 19
PstI C_TGCA'G
Cuts at: 830 6848 7161 7312 9249 9289 10949 830
Size: 6018 313 151 1937 40 1660 3478
Fragments arranged by size:
6018 3478 1937 1660 313 151 40
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 13597
PvuII CAG'CTG
Cuts at: 2671 5246 6182 6843 7371 8744 9284 2671
Size: 2575 936 661 528 1373 540 6984
Fragments arranged by size:
6984 2575 1373 936 661 540 528
RsrII CG'GwC_CG
Cuts at: 10039 10039
Size: 13597
SalI G'TCGA_C
Cuts at: 1936 2328 7557 10939 1936
Size: 392 5229 3382 4594
Fragments arranged by size:
5229 4594 3382 392
SapI GCTCTTCn'nnn_
Cuts at: 3952 6526 7806 3952
Size: 2574 1280 9743
Fragments arranged by size:
9743 2574 1280
SbfI CC_TGCA'GG
Cuts at: 10949 10949
Size: 13597
ScaI AGT'ACT
Cuts at: 2086 4108 6130 7078 9563 11845 13557 2086
Size: 2022 2022 948 2485 2282 1712 2126
Fragments arranged by size:
2485 2282 2126 2022 2022 1712 948
SfcI C'TryA_G
Cuts at: 826 1045 2736 2787 2940 2991 3768 4873 5019
Size: 219 1691 51 153 51 777 1105 146
Cuts at: 5019 5718 6553 6844 7157 7308 8007 9245 9285
Size: 699 835 291 313 151 699 1238 40
Cuts at: 9285 10251 10945 12080 12761 12952 826
Size: 966 694 1135 681 191 1471
Fragments arranged by size:
1691 1471 1238 1135 1105 966 835 777
699 699 694 681 313 291 219 191
153 151 146 51 51 40
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13597
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 13597
SmaI CCC'GGG
Cuts at: 1872 10924 1872
Size: 9052 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3311 4889 5222 5524 11701 12602
Size: 197 926 1368 1578 333 302 6177 901
Cuts at: 12602 12864 820
Size: 262 1553
Fragments arranged by size:
6177 1578 1553 1368 926 901 333 302
262 197
SnaBI TAC'GTA
Cuts at: 493 10287 11388 493
Size: 9794 1101 2702
Fragments arranged by size:
9794 2702 1101
SpeI A'CTAG_T
Cuts at: 152 2645 4022 4109 5345 152
Size: 2493 1377 87 1236 8404
Fragments arranged by size:
8404 2493 1377 1236 87
SphI G_CATG'C
Cuts at: 1132 6290 10955 1132
Size: 5158 4665 3774
Fragments arranged by size:
5158 4665 3774
SspI AAT'ATT
Cuts at: 5 52 9151 9298 11521 5
Size: 47 9099 147 2223 2081
Fragments arranged by size:
9099 2223 2081 147 47
StuI AGG'CCT
Cuts at: 1517 4495 4681 5465 6356 9614 9883 1517
Size: 2978 186 784 891 3258 269 5231
Fragments arranged by size:
5231 3258 2978 891 784 269 186
StyI C'CwwG_G
Cuts at: 513 1065 2004 2360 3156 3952 6708 6766 7191
Size: 552 939 356 796 796 2756 58 425
Cuts at: 7191 7931 8512 8895 9878 10242 11036 513
Size: 740 581 383 983 364 794 3074
Fragments arranged by size:
3074 2756 983 939 796 796 794 740
581 552 425 383 364 356 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2373 4046 7287 8407 11624 11641 11794
Size: 1255 793 1673 3241 1120 3217 17 153
Cuts at: 11794 11979 12424 325
Size: 185 445 1498
Fragments arranged by size:
3241 3217 1673 1498 1255 1120 793 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2084 2439 2545
Size: 274 80 33 51 1443 107 355 106
Cuts at: 2545 3326 4106 4723 6128 6973 7076 9561 10329
Size: 781 780 617 1405 845 103 2485 768
Cuts at: 10329 11843 13555 96
Size: 1514 1712 138
Fragments arranged by size:
2485 1712 1514 1443 1405 845 781 780
768 617 355 274 138 107 106 103
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2761 2793 4415 4447 4741 4773 6214 6246 6250
Size: 32 1622 32 294 32 1441 32 4
Cuts at: 6250 6282 8299 8331 10169 10201 10252 10284 13557
Size: 32 2017 32 1838 32 51 32 3273
Cuts at: 13557 13589 2761
Size: 32 2769
Fragments arranged by size:
3273 2769 2017 1838 1622 1441 294 51
32 32 32 32 32 32 32 32
32 4
Tth111I GACn'n_nGTC
Cuts at: 1316 7478 1316
Size: 6162 7435
XbaI T'CTAG_A
Cuts at: 8428 10933 8428
Size: 2505 11092
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3839 3839
Size: 13597
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 13597
XmaI C'CCGG_G
Cuts at: 1870 10922 1870
Size: 9052 4545
XmnI GAAnn'nnTTC
Cuts at: 8436 10212 10587 11726 8436
Size: 1776 375 1139 10307
Fragments arranged by size:
10307 1776 1139 375
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 11405 276
Size: 53 83 186 936 9871 2468
Fragments arranged by size:
9871 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII RsrII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
ApaI AscI BaeI BbeI BsiWI BstXI DraIII EcoICRI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI