Restriction map for
pFN21AE3827
(Circular) (Six-base) MAPSORT of: pFN21AE3827.seq Check: 4431 from: 1 to: 13156
pFN21AE3827 13156 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 10966 278
Size: 53 83 186 936 9430 2468
Fragments arranged by size:
9430 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 8580 10499 1937
Size: 6643 1919 4594
Fragments arranged by size:
6643 4594 1919
Acc65I G'GTAC_C
Cuts at: 10477 10477
Size: 13156
AclI AA'CG_TT
Cuts at: 1563 8290 11283 11656 1563
Size: 6727 2993 373 3063
Fragments arranged by size:
6727 3063 2993 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2088 2103 2280 2453 3024 3191 3326 3482
Size: 456 15 177 173 571 167 135 156
Cuts at: 3482 4556 4752 4818 4922 5649 6084 6120 6155
Size: 1074 196 66 104 727 435 36 35
Cuts at: 6155 6651 7550 8756 9293 11219 12603 12871 1632
Size: 496 899 1206 537 1926 1384 268 1917
Fragments arranged by size:
1926 1917 1384 1206 1074 899 727 571
537 496 456 435 268 196 177 173
167 156 135 104 66 36 35 15
AfeI AGC'GCT
Cuts at: 9616 9616
Size: 13156
AflII C'TTAA_G
Cuts at: 820 1017 4976 820
Size: 197 3959 9000
Fragments arranged by size:
9000 3959 197
AflIII A'CryG_T
Cuts at: 1203 5835 7770 12055 13032 1203
Size: 4632 1935 4285 977 1327
Fragments arranged by size:
4632 4285 1935 1327 977
AgeI A'CCGG_T
Cuts at: 11987 11987
Size: 13156
AhdI GACnn_n'nnGTC
Cuts at: 3057 3313 8845 11885 3057
Size: 256 5532 3040 4328
Fragments arranged by size:
5532 4328 3040 256
AleI CACnn'nnGTG
Cuts at: 1166 6632 1166
Size: 5466 7690
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2238 5592 6798 1184
Size: 1054 3354 1206 7542
Fragments arranged by size:
7542 3354 1206 1054
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2140 2172 4432 4464 4852 4884 5207 5239 2140
Size: 32 2260 32 388 32 323 32 10057
Fragments arranged by size:
10057 2260 388 323 32 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3437 3779 3998 4988 5053 9667 9766 9913 10899
Size: 342 219 990 65 4614 99 147 986
Cuts at: 10899 12471 3437
Size: 1572 4122
Fragments arranged by size:
4614 4122 1572 990 986 342 219 147
99 65
ApaI G_GGCC'C
Cuts at: 5629 5629
Size: 13156
ApaLI G'TGCA_C
Cuts at: 2964 3566 11213 12369 2964
Size: 602 7647 1156 3751
Fragments arranged by size:
7647 3751 1156 602
ApoI r'AATT_y
Cuts at: 1493 2392 3757 3916 4495 7787 8430 9042 10465
Size: 899 1365 159 579 3292 643 612 1423
Cuts at: 10465 10691 10843 12952 1493
Size: 226 152 2109 1697
Fragments arranged by size:
3292 2109 1697 1423 1365 899 643 612
579 226 159 152
AseI AT'TA_AT
Cuts at: 160 1032 7088 11710 160
Size: 872 6056 4622 1606
Fragments arranged by size:
6056 4622 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13156
AvaI C'yCGr_G
Cuts at: 1870 1943 9090 9709 9800 10481 1870
Size: 73 7147 619 91 681 4545
Fragments arranged by size:
7147 4545 681 619 91 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5494 5527 5494
Size: 33 13123
BamHI G'GATC_C
Cuts at: 2920 6413 10104 10486 10853 2920
Size: 3493 3691 382 367 5223
Fragments arranged by size:
5223 3691 3493 382 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2427 4324 5056 6759 10477
Size: 325 217 627 640 1897 732 1703 3718
Cuts at: 10477 11932 618
Size: 1455 1842
Fragments arranged by size:
3718 1897 1842 1703 1455 732 640 627
325 217
BanII G_rGCy'C
Cuts at: 1391 2581 2976 3041 3462 5313 5589 5607 5629
Size: 1190 395 65 421 1851 276 18 22
Cuts at: 5629 5659 6239 6665 6906 9091 9609 9716 9936
Size: 30 580 426 241 2185 518 107 220
Cuts at: 9936 10137 10475 1391
Size: 201 338 4072
Fragments arranged by size:
4072 2185 1851 1190 580 518 426 421
395 338 276 241 220 201 107 65
30 22 18
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2271 3648 4140 4533 7382 8306 928
Size: 971 372 1377 492 393 2849 924 5778
Fragments arranged by size:
5778 2849 1377 971 924 492 393 372
BbvCI CC'TCA_GC
Cuts at: 2534 2985 5526 6558 9611 2534
Size: 451 2541 1032 3053 6079
Fragments arranged by size:
6079 3053 2541 1032 451
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2654 2688 4022 4056 11347 11381 1903
Size: 34 717 34 1334 34 7291 34 3678
Fragments arranged by size:
7291 3678 1334 717 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2597 4168 8168 11048 12258 2597
Size: 1571 4000 2880 1210 3495
Fragments arranged by size:
4000 3495 2880 1571 1210
BclI T'GATC_A
Cuts at: 1548 3190 7000 8151 8916 1548
Size: 1642 3810 1151 765 5788
Fragments arranged by size:
5788 3810 1642 1151 765
BfrBI ATG'CAT
Cuts at: 2509 2509
Size: 13156
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3942 4709 5105
Size: 107 122 71 631 605 2270 767 396
Cuts at: 5105 9872 11767 136
Size: 4767 1895 1525
Fragments arranged by size:
4767 2270 1895 1525 767 631 605 396
122 107 71
BglII A'GATC_T
Cuts at: 7019 8533 9490 10446 13151 7019
Size: 1514 957 956 2705 7024
Fragments arranged by size:
7024 2705 1514 957 956
BlpI GC'TnA_GC
Cuts at: 5307 9674 10040 10573 5307
Size: 4367 366 533 7890
Fragments arranged by size:
7890 4367 533 366
Bme1580I G_kGCm'C
Cuts at: 1790 2935 2968 3239 3570 3616 5629 6764 7392
Size: 1145 33 271 331 46 2013 1135 628
Cuts at: 7392 9953 11217 12373 1790
Size: 2561 1264 1156 2573
Fragments arranged by size:
2573 2561 2013 1264 1156 1145 1135 628
331 271 46 33
BmgBI CAC'GTC
Cuts at: 1318 2544 3565 3571 6055 6601 1318
Size: 1226 1021 6 2484 546 7873
Fragments arranged by size:
7873 2484 1226 1021 546 6
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3370 3407 6278
Size: 416 53 83 399 24 1953 37 2871
Cuts at: 6278 6339 7907 8471 9787 10116 10887 11845 442
Size: 61 1568 564 1316 329 771 958 1753
Fragments arranged by size:
2871 1953 1753 1568 1316 958 771 564
416 399 329 83 61 53 37 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 13156
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6301 9223 10156 6301
Size: 2922 933 9301
Fragments arranged by size:
9301 2922 933
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2697 2713 2829 3408 3573 3729 3993 4620
Size: 1458 16 116 579 165 156 264 627
Cuts at: 4620 5510 5823 6989 7770 8207 9767 9920 9989
Size: 890 313 1166 781 437 1560 153 69
Cuts at: 9989 11816 1239
Size: 1827 2579
Fragments arranged by size:
2579 1827 1560 1458 1166 890 781 627
579 437 313 264 165 156 153 116
69 16
Bpu10I CC'TnA_GC
Cuts at: 2534 2985 3320 4773 5526 6030 6558 9611 2534
Size: 451 335 1453 753 504 528 3053 6079
Fragments arranged by size:
6079 3053 1453 753 528 504 451 335
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2861 4292 5016 8039 8569 10019 11281 12146 12444
Size: 1431 724 3023 530 1450 1262 865 298
Cuts at: 12444 2861
Size: 3573
Fragments arranged by size:
3573 3023 1450 1431 1262 865 724 530
298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2165 5710 9407 11819 882
Size: 617 666 3545 3697 2412 2219
Fragments arranged by size:
3697 3545 2412 2219 666 617
BsaAI yAC'GTr
Cuts at: 493 1204 8717 10947 493
Size: 711 7513 2230 2702
Fragments arranged by size:
7513 2702 2230 711
BsaBI GATnn'nnATC
Cuts at: 2787 8156 8993 10103 13150 2787
Size: 5369 837 1110 3047 2793
Fragments arranged by size:
5369 3047 2793 1110 837
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2609 10963 11345 11980
Size: 53 83 186 936 1076 8354 382 635
Cuts at: 11980 275
Size: 1451
Fragments arranged by size:
8354 1451 1076 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2968 6410 6687 6744 11589 11987 12261 12408 12819
Size: 3442 277 57 4845 398 274 147 411
Cuts at: 12819 2968
Size: 3305
Fragments arranged by size:
4845 3442 3305 411 398 277 274 147
57
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2164 2194 3321 3351 4432
Size: 30 165 30 761 30 1127 30 1081
Cuts at: 4432 4462 4852 4882 5105 5135 6834 6864 1178
Size: 30 390 30 223 30 1699 30 7470
Fragments arranged by size:
7470 1699 1127 1081 761 390 223 165
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2681 3101 3104 3743 5184 5765 6008 6620
Size: 1495 420 3 639 1441 581 243 612
Cuts at: 6620 6623 6626 6855 8912 9599 9818 9899 9902
Size: 3 3 229 2057 687 219 81 3
Cuts at: 9902 9944 10145 1186
Size: 42 201 4197
Fragments arranged by size:
4197 2057 1495 1441 687 639 612 581
420 243 229 219 201 81 42 3
3 3 3
BseYI C'CCAG_C
Cuts at: 2524 4097 5629 6699 7701 9876 12359 12855 2524
Size: 1573 1532 1070 1002 2175 2483 496 2825
Fragments arranged by size:
2825 2483 2175 1573 1532 1070 1002 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2234 2633 4011 6794 9202 9914 10134
Size: 573 498 399 1378 2783 2408 712 220
Cuts at: 10134 1163
Size: 4185
Fragments arranged by size:
4185 2783 2408 1378 712 573 498 399
220
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 10606 11367 12395 1540
Size: 275 186 8605 761 1028 2301
Fragments arranged by size:
8605 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2581 2968 3020 3570 5115 5589 5607 5684 5809
Size: 387 52 550 1545 474 18 77 125
Cuts at: 5809 6665 6906 9609 9716 9936 11217 11302 12373
Size: 856 241 2703 107 220 1281 85 1071
Cuts at: 12373 2581
Size: 3364
Fragments arranged by size:
3364 2703 1545 1281 1071 856 550 474
387 241 220 125 107 85 77 52
18
BsmI GAATG_Cn'
Cuts at: 2420 2677 3303 5389 8287 8746 9451 10667 10760
Size: 257 626 2086 2898 459 705 1216 93
Cuts at: 10760 2420
Size: 4816
Fragments arranged by size:
4816 2898 2086 1216 705 626 459 257
93
BsmBI CGTCTCn'nnnn_
Cuts at: 2805 3969 8621 2805
Size: 1164 4652 7340
Fragments arranged by size:
7340 4652 1164
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2581 2935 2968 2976 3020 3041 3239
Size: 399 791 354 33 8 44 21 198
Cuts at: 3239 3462 3570 3616 5115 5313 5589 5607 5629
Size: 223 108 46 1499 198 276 18 22
Cuts at: 5629 5659 5684 5809 6239 6665 6764 6906 7392
Size: 30 25 125 430 426 99 142 486
Cuts at: 7392 9091 9609 9716 9936 9953 10137 10475 11217
Size: 1699 518 107 220 17 184 338 742
Cuts at: 11217 11302 12373 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1699 1499 1071 791 742 518 486
430 426 399 354 338 276 223 220
198 198 184 142 125 108 107 99
85 46 44 33 30 25 22 21
18 17 8
BspEI T'CCGG_A
Cuts at: 6687 12819 6687
Size: 6132 7024
BspHI T'CATG_A
Cuts at: 2188 2791 3462 4894 8061 8655 11045 12775 2188
Size: 603 671 1432 3167 594 2390 1730 2569
Fragments arranged by size:
3167 2569 2390 1730 1432 671 603 594
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3655 9565 9783 10511 844
Size: 2811 5910 218 728 3489
Fragments arranged by size:
5910 3489 2811 728 218
BsrBI CCG'CTC
Cuts at: 1394 1823 5126 8191 9240 9859 9925 10036 10395
Size: 429 3303 3065 1049 619 66 111 359
Cuts at: 10395 11043 1394
Size: 648 3507
Fragments arranged by size:
3507 3303 3065 1049 648 619 429 359
111 66
BsrDI GCAATG_nn'
Cuts at: 65 1248 2175 3852 7765 11651 11825 65
Size: 1183 927 1677 3913 3886 174 1396
Fragments arranged by size:
3913 3886 1677 1396 1183 927 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 11800 11987 1700
Size: 110 9990 187 2869
Fragments arranged by size:
9990 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2135 6185 7046 8073 96
Size: 2039 4050 861 1027 5179
Fragments arranged by size:
5179 4050 2039 1027 861
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 13156
BssSI C'ACGA_G
Cuts at: 9837 11216 12228 9837
Size: 1379 1012 10765
Fragments arranged by size:
10765 1379 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2321 3024 3998 4967 794
Size: 1527 703 974 969 8983
Fragments arranged by size:
8983 1527 974 969 703
BstBI TT'CG_AA
Cuts at: 4499 11960 4499
Size: 7461 5695
BstEII G'GTnAC_C
Cuts at: 1184 4798 1184
Size: 3614 9542
BstXI CCAn_nnnn'nTGG
Cuts at: 2691 3631 5599 5621 7927 2691
Size: 940 1968 22 2306 7920
Fragments arranged by size:
7920 2306 1968 940 22
BstYI r'GATC_y
Cuts at: 1972 2920 4108 6413 6964 7019 8095 8533 8935
Size: 948 1188 2305 551 55 1076 438 402
Cuts at: 8935 9303 9490 10104 10446 10486 10853 11238 11255
Size: 368 187 614 342 40 367 385 17
Cuts at: 11255 12707 12793 12805 13151 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2305 1977 1452 1188 1076 948 614 551
438 402 385 368 367 346 342 187
86 55 40 17 12
Bsu36I CC'TnA_GG
Cuts at: 5730 5949 6656 10128 5730
Size: 219 707 3472 8758
Fragments arranged by size:
8758 3472 707 219
BtgI C'CryG_G
Cuts at: 513 1065 2442 2572 2976 5514 5958 6544 7593
Size: 552 1377 130 404 2538 444 586 1049
Cuts at: 7593 513
Size: 6076
Fragments arranged by size:
6076 2538 1377 1049 586 552 444 404
130
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 6741 487
Size: 683 56 5515 6902
Fragments arranged by size:
6902 5515 683 56
BtsI GCAGTG_nn'
Cuts at: 3331 3435 4387 4557 5021 5807 6475 7492 7586
Size: 104 952 170 464 786 668 1017 94
Cuts at: 7586 8507 10672 11466 11486 3331
Size: 921 2165 794 20 5001
Fragments arranged by size:
5001 2165 1017 952 921 794 786 668
464 170 104 94 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 13156
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 8299 8334 529
Size: 35 7735 35 5351
Fragments arranged by size:
7735 5351 35 35
DraI TTT'AAA
Cuts at: 2836 6495 8295 8865 10460 2836
Size: 3659 1800 570 1595 5532
Fragments arranged by size:
5532 3659 1800 1595 570
DraIII CAC_nnn'GTG
Cuts at: 2448 3024 4958 6347 2448
Size: 576 1934 1389 9257
Fragments arranged by size:
9257 1934 1389 576
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3569 12163 809
Size: 507 2253 8594 1802
Fragments arranged by size:
8594 2253 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2569 2947 4517
Size: 54 1425 50 275 18 739 378 1570
Cuts at: 4517 5076 9484 9768 10603 11492 13121 8
Size: 559 4408 284 835 889 1629 43
Fragments arranged by size:
4408 1629 1570 1425 889 835 739 559
378 284 275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 10603 1537
Size: 275 8791 4090
Fragments arranged by size:
8791 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2064 3000 3141 4684 4837 4845 5311 6222
Size: 711 936 141 1543 153 8 466 911
Cuts at: 6222 6381 6894 6954 7176 7229 7292 8660 9141
Size: 159 513 60 222 53 63 1368 481
Cuts at: 9141 9239 9246 9465 10437 11086 1353
Size: 98 7 219 972 649 3423
Fragments arranged by size:
3423 1543 1368 972 936 911 711 649
513 481 466 222 219 159 153 141
98 63 60 53 8 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8108 11743 12117 12263 387
Size: 1064 138 6519 3635 374 146 1280
Fragments arranged by size:
6519 3635 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2579 5587 5605 6663 6904 9607 9714 9934 2579
Size: 3008 18 1058 241 2703 107 220 5801
Fragments arranged by size:
5801 3008 2703 1058 241 220 107 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2088 2103 2280 2453 2697 2713 2829
Size: 393 456 15 177 173 244 16 116
Cuts at: 2829 3024 3191 3326 3408 3482 3573 3729 3993
Size: 195 167 135 82 74 91 156 264
Cuts at: 3993 4556 4620 4752 4818 4922 5510 5649 5823
Size: 563 64 132 66 104 588 139 174
Cuts at: 5823 6084 6120 6155 6651 6989 7550 7770 8207
Size: 261 36 35 496 338 561 220 437
Cuts at: 8207 8756 9293 9767 9920 9989 11219 11816 12603
Size: 549 537 474 153 69 1230 597 787
Cuts at: 12603 12871 1239
Size: 268 1524
Fragments arranged by size:
1524 1230 787 597 588 563 561 549
537 496 474 456 437 393 338 268
264 261 244 220 195 177 174 173
167 156 153 139 135 132 116 104
91 82 74 69 66 64 36 35
16 15
EcoNI CCTnn'n_nnAGG
Cuts at: 6688 8558 9524 9917 10070 6688
Size: 1870 966 393 153 9774
Fragments arranged by size:
9774 1870 966 393 153
EcoO109I rG'GnC_Cy
Cuts at: 2236 2372 4625 5625 5626 5861 6026 6704 7418
Size: 136 2253 1000 1 235 165 678 714
Cuts at: 7418 9269 9763 9865 9910 10063 10241 10303 2236
Size: 1851 494 102 45 153 178 62 5089
Fragments arranged by size:
5089 2253 1851 1000 714 678 494 235
178 165 153 136 102 62 45 1
EcoRI G'AATT_C
Cuts at: 2392 3757 8430 9042 10465 10843 2392
Size: 1365 4673 612 1423 378 4705
Fragments arranged by size:
4705 4673 1423 1365 612 378
EcoRV GAT'ATC
Cuts at: 6406 6406
Size: 13156
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2255 4760 6317 7580 8792 8842 9125 2255
Size: 2505 1557 1263 1212 50 283 6286
Fragments arranged by size:
6286 2505 1557 1263 1212 283 50
FspI TGC'GCA
Cuts at: 11662 11662
Size: 13156
HaeII r_GCGC'y
Cuts at: 1678 9618 9689 9760 12303 1678
Size: 7940 71 71 2543 2531
Fragments arranged by size:
7940 2543 2531 71 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2164 2196 2728 2760 2875
Size: 32 135 32 592 32 532 32 115
Cuts at: 2875 2907 3043 3075 3299 3331 3339 3371 3833
Size: 32 136 32 224 32 8 32 462
Cuts at: 3833 3865 5312 5344 5449 5481 6197 6229 6834
Size: 32 1447 32 105 32 716 32 605
Cuts at: 6834 6866 6956 6985 6988 7017 7568 7600 7757
Size: 32 90 29 3 29 551 32 157
Cuts at: 7757 7789 7901 7933 8080 8112 8633 8665 8831
Size: 32 112 32 147 32 521 32 166
Cuts at: 8831 8863 8920 8952 9313 9345 11797 11829 11871
Size: 32 57 32 361 32 2452 32 42
Cuts at: 11871 11903 1373
Size: 32 2626
Fragments arranged by size:
2626 2452 1447 716 605 592 551 532
521 462 361 224 166 157 147 136
135 115 112 105 90 57 42 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 29 29
8 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2541 4007 6937 7384 7842 8850
Size: 988 281 603 1466 2930 447 458 1008
Cuts at: 8850 10344 10500 10746 669
Size: 1494 156 246 3079
Fragments arranged by size:
3079 2930 1494 1466 1008 988 603 458
447 281 246 156
HindIII A'AGCT_T
Cuts at: 748 8708 748
Size: 7960 5196
HpaI GTT'AAC
Cuts at: 10344 10746 10344
Size: 402 12754
KpnI G_GTAC'C
Cuts at: 10481 10481
Size: 13156
MfeI C'AATT_G
Cuts at: 10755 10755
Size: 13156
MluI A'CGCG_T
Cuts at: 13032 13032
Size: 13156
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2733 2875 5185 5425 6244 6832 7928 9057
Size: 1428 142 2310 240 819 588 1096 1129
Cuts at: 9057 9123 9267 12270 12454 1305
Size: 66 144 3003 184 2007
Fragments arranged by size:
3003 2310 2007 1428 1129 1096 819 588
240 184 144 142 66
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2949 5078 9486 9770 10
Size: 54 1425 343 1117 2129 4408 284 3396
Fragments arranged by size:
4408 3396 2129 1425 1117 343 284 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5597 5619 6468 6632 8660 8927 11114
Size: 648 4431 22 849 164 2028 267 2187
Cuts at: 11114 11473 11632 518
Size: 359 159 2042
Fragments arranged by size:
4431 2187 2042 2028 849 648 359 267
164 159 22
MspA1I CmG'CkG
Cuts at: 1606 2574 3201 3551 3776 4913 5508 5633 5801
Size: 968 627 350 225 1137 595 125 168
Cuts at: 5801 6699 7428 7927 8461 8985 10018 10048 10572
Size: 898 729 499 534 524 1033 30 524
Cuts at: 10572 11249 12397 12642 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 1137 1033 968 898 729 677
627 595 534 524 524 499 350 245
225 168 125 30
NcoI C'CATG_G
Cuts at: 513 1065 5514 6544 513
Size: 552 4449 1030 7125
Fragments arranged by size:
7125 4449 1030 552
NdeI CA'TA_TG
Cuts at: 387 8305 387
Size: 7918 5238
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 13156
NotI GC'GGCC_GC
Cuts at: 10603 10603
Size: 13156
NsiI A_TGCA'T
Cuts at: 2511 2511
Size: 13156
NspI r_CATG'y
Cuts at: 1132 1229 4005 5839 7774 10514 12059 1132
Size: 97 2776 1834 1935 2740 1545 2229
Fragments arranged by size:
2776 2740 2229 1935 1834 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3032 5227 1348
Size: 1684 2195 9277
Fragments arranged by size:
9277 2195 1684
PciI A'CATG_T
Cuts at: 5835 7770 12055 5835
Size: 1935 4285 6936
Fragments arranged by size:
6936 4285 1935
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2448 2807 3483 4871 5053 6550 6920 9667
Size: 1340 359 676 1388 182 1497 370 2747
Cuts at: 9667 9766 1108
Size: 99 4498
Fragments arranged by size:
4498 2747 1497 1388 1340 676 370 359
182 99
PfoI T'CCnGG_A
Cuts at: 2280 2690 2806 4870 6255 7105 9165 2280
Size: 410 116 2064 1385 850 2060 6271
Fragments arranged by size:
6271 2064 2060 1385 850 410 116
PmeI GTTT'AAAC
Cuts at: 10460 10460
Size: 13156
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3444 3476 4432 4464 4661 4693 4852 4884 10202
Size: 32 956 32 197 32 159 32 5318
Cuts at: 10202 10234 11225 11257 12738 12770 3444
Size: 32 991 32 1481 32 3830
Fragments arranged by size:
5318 3830 1481 991 956 197 159 32
32 32 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2372 4625 5861 7418 9269 9763 9910 10063 2372
Size: 2253 1236 1557 1851 494 147 153 5465
Fragments arranged by size:
5465 2253 1851 1557 1236 494 153 147
PshAI GACnn'nnGTC
Cuts at: 1613 10068 1613
Size: 8455 4701
PsiI TTA'TAA
Cuts at: 10726 10726
Size: 13156
PspOMI G'GGCC_C
Cuts at: 5625 5625
Size: 13156
PspXI vC'TCGA_Gb
Cuts at: 9800 9800
Size: 13156
PstI C_TGCA'G
Cuts at: 830 3442 4567 4771 5183 5571 9624 9639 9780
Size: 2612 1125 204 412 388 4053 15 141
Cuts at: 9780 10508 830
Size: 728 3478
Fragments arranged by size:
4053 3478 2612 1125 728 412 388 204
141 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 13156
PvuII CAG'CTG
Cuts at: 3201 3551 3776 4913 5508 5633 6699 7428 7927
Size: 350 225 1137 595 125 1066 729 499
Cuts at: 7927 8985 10018 3201
Size: 1058 1033 6339
Fragments arranged by size:
6339 1137 1066 1058 1033 729 595 499
350 225 125
RsrII CG'GwC_CG
Cuts at: 7641 7641
Size: 13156
SacI G_AGCT'C
Cuts at: 2581 5589 5607 6665 6906 9609 9716 9936 2581
Size: 3008 18 1058 241 2703 107 220 5801
Fragments arranged by size:
5801 3008 2703 1058 241 220 107 18
SacII CC_GC'GG
Cuts at: 2575 2575
Size: 13156
SalI G'TCGA_C
Cuts at: 1936 10498 1936
Size: 8562 4594
SanDI GG'GwC_CC
Cuts at: 4625 7418 4625
Size: 2793 10363
SapI GCTCTTCn'nnn_
Cuts at: 4845 6381 6894 7292 4845
Size: 1536 513 398 10709
Fragments arranged by size:
10709 1536 513 398
SbfI CC_TGCA'GG
Cuts at: 10508 10508
Size: 13156
ScaI AGT'ACT
Cuts at: 2143 4354 5908 5944 6779 11404 13116 2143
Size: 2211 1554 36 835 4625 1712 2183
Fragments arranged by size:
4625 2211 2183 1712 1554 835 36
SexAI A'CCwGG_T
Cuts at: 9543 9543
Size: 13156
SfcI C'TryA_G
Cuts at: 826 1045 3109 3438 4467 4563 4767 5179 5411
Size: 219 2064 329 1029 96 204 412 232
Cuts at: 5411 5567 6111 6538 7429 8577 9620 9635 9776
Size: 156 544 427 891 1148 1043 15 141
Cuts at: 9776 10504 11639 12320 12511 826
Size: 728 1135 681 191 1471
Fragments arranged by size:
2064 1471 1148 1135 1043 1029 891 728
681 544 427 412 329 232 219 204
191 156 141 96 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 9872 9872
Size: 13156
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13156
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 13156
SmaI CCC'GGG
Cuts at: 1872 10483 1872
Size: 8611 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2876 4271 4976 5031 8054 8548
Size: 197 926 933 1395 705 55 3023 494
Cuts at: 8548 9800 9998 11260 12161 12423 820
Size: 1252 198 1262 901 262 1553
Fragments arranged by size:
3023 1553 1395 1262 1252 933 926 901
705 494 262 198 197 55
SnaBI TAC'GTA
Cuts at: 493 10947 493
Size: 10454 2702
SpeI A'CTAG_T
Cuts at: 152 5237 152
Size: 5085 8071
SphI G_CATG'C
Cuts at: 1132 10514 1132
Size: 9382 3774
SspI AAT'ATT
Cuts at: 5 52 3541 7683 8496 11080 5
Size: 47 3489 4142 813 2584 2081
Fragments arranged by size:
4142 3489 2584 2081 813 47
StuI AGG'CCT
Cuts at: 1517 3246 4542 5828 1517
Size: 1729 1296 1286 8845
Fragments arranged by size:
8845 1729 1296 1286
StyI C'CwwG_G
Cuts at: 513 1065 2625 4395 4923 5100 5514 5580 6544
Size: 552 1560 1770 528 177 414 66 964
Cuts at: 6544 7626 8364 8685 10595 513
Size: 1082 738 321 1910 3074
Fragments arranged by size:
3074 1910 1770 1560 1082 964 738 552
528 414 321 177 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2814 3970 5354 5507 6358 6465 6927
Size: 1255 1234 1156 1384 153 851 107 462
Cuts at: 6927 7629 9374 11183 11200 11353 11538 11983 325
Size: 702 1745 1809 17 153 185 445 1498
Fragments arranged by size:
1809 1745 1498 1384 1255 1234 1156 851
702 462 445 185 153 153 107 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2135 2141 3512
Size: 274 80 33 51 1443 158 6 1371
Cuts at: 3512 4352 5906 5942 6185 6298 6777 7046 8073
Size: 840 1554 36 243 113 479 269 1027
Cuts at: 8073 8820 11402 13114 96
Size: 747 2582 1712 138
Fragments arranged by size:
2582 1712 1554 1443 1371 1027 840 747
479 274 269 243 158 138 113 80
51 36 33 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3321 3353 5105 5137 5544 5576 7113 7145 8653
Size: 32 1752 32 407 32 1537 32 1508
Cuts at: 8653 8685 13116 13148 3321
Size: 32 4431 32 3329
Fragments arranged by size:
4431 3329 1752 1537 1508 407 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3353 1316
Size: 2037 11119
XbaI T'CTAG_A
Cuts at: 6823 6874 10492 6823
Size: 51 3618 9487
Fragments arranged by size:
9487 3618 51
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3383 5767 5854 6352 7411 3383
Size: 2384 87 498 1059 9128
Fragments arranged by size:
9128 2384 1059 498 87
XhoI C'TCGA_G
Cuts at: 1943 9800 1943
Size: 7857 5299
XmaI C'CCGG_G
Cuts at: 1870 10481 1870
Size: 8611 4545
XmnI GAAnn'nnTTC
Cuts at: 2268 7448 9042 9108 11285 2268
Size: 5180 1594 66 2177 4139
Fragments arranged by size:
5180 4139 2177 1594 66
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 10964 276
Size: 53 83 186 936 9430 2468
Fragments arranged by size:
9430 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AscI AvrII BbeI BsiWI BstZ17I FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PsrI
SfoI SrfI SwaI