Restriction map for
pFN21AE3826
(Circular) (Six-base) MAPSORT of: pFN21AE3826.seq Check: 1054 from: 1 to: 12718
pFN21AE3826 12718 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2779 3269 5760 5877 5978 6045 7290 8220 9284
Size: 490 2491 117 101 67 1245 930 1064
Cuts at: 9284 2779
Size: 6213
Fragments arranged by size:
6213 2491 1245 1064 930 490 117 101
67
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8088 10528 278
Size: 53 83 186 936 6552 2440 2468
Fragments arranged by size:
6552 2468 2440 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6147 6515 7470 9144 9201 10061 1937
Size: 4210 368 955 1674 57 860 4594
Fragments arranged by size:
4594 4210 1674 955 860 368 57
Acc65I G'GTAC_C
Cuts at: 4194 10039 4194
Size: 5845 6873
AclI AA'CG_TT
Cuts at: 1563 10845 11218 1563
Size: 9282 373 3063
Fragments arranged by size:
9282 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3590 4223 4621 4795 4913 4931 5233 5615
Size: 1958 633 398 174 118 18 302 382
Cuts at: 5615 6427 6842 8237 10781 12165 12433 1632
Size: 812 415 1395 2544 1384 268 1917
Fragments arranged by size:
2544 1958 1917 1395 1384 812 633 415
398 382 302 268 174 118 18
AfeI AGC'GCT
Cuts at: 6914 7355 8777 6914
Size: 441 1422 10855
Fragments arranged by size:
10855 1422 441
AflII C'TTAA_G
Cuts at: 820 1017 9605 820
Size: 197 8588 3933
Fragments arranged by size:
8588 3933 197
AflIII A'CryG_T
Cuts at: 1203 2762 3189 3657 4464 7573 8364 9696 11617
Size: 1559 427 468 807 3109 791 1332 1921
Cuts at: 11617 12594 1203
Size: 977 1327
Fragments arranged by size:
3109 1921 1559 1332 1327 977 807 791
468 427
AgeI A'CCGG_T
Cuts at: 11549 11549
Size: 12718
AhdI GACnn_n'nnGTC
Cuts at: 6193 7345 11447 6193
Size: 1152 4102 7464
Fragments arranged by size:
7464 4102 1152
AleI CACnn'nnGTG
Cuts at: 1166 3209 4784 6227 6278 7601 8699 1166
Size: 2043 1575 1443 51 1323 1098 5185
Fragments arranged by size:
5185 2043 1575 1443 1323 1098 51
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3227 3986 4757 5186 5220 6188 6491 7013
Size: 2043 759 771 429 34 968 303 522
Cuts at: 7013 7076 7088 7619 1184
Size: 63 12 531 6283
Fragments arranged by size:
6283 2043 968 771 759 531 522 429
303 63 34 12
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3875 3907 6455 6487 7085 7117 8351 8383 9053
Size: 32 2548 32 598 32 1234 32 670
Cuts at: 9053 9085 3875
Size: 32 7508
Fragments arranged by size:
7508 2548 1234 670 598 32 32 32
32 32
AlwNI CAG_nnn'CTG
Cuts at: 2281 2377 2584 3430 3526 4267 4381 5045 5296
Size: 96 207 846 96 741 114 664 251
Cuts at: 5296 5698 5737 5902 6508 7021 7642 7735 8077
Size: 402 39 165 606 513 621 93 342
Cuts at: 8077 8212 8443 10461 12033 2281
Size: 135 231 2018 1572 2966
Fragments arranged by size:
2966 2018 1572 846 741 664 621 606
513 402 342 251 231 207 165 135
114 96 96 93 39
ApaI G_GGCC'C
Cuts at: 4698 7393 7541 7856 8844 9761 4698
Size: 2695 148 315 988 917 7655
Fragments arranged by size:
7655 2695 988 917 315 148
ApaLI G'TGCA_C
Cuts at: 4355 5864 6163 10775 11931 4355
Size: 1509 299 4612 1156 5142
Fragments arranged by size:
5142 4612 1509 1156 299
ApoI r'AATT_y
Cuts at: 1493 8960 10027 10253 10405 12514 1493
Size: 7467 1067 226 152 2109 1697
Fragments arranged by size:
7467 2109 1697 1067 226 152
AseI AT'TA_AT
Cuts at: 160 1032 11272 160
Size: 872 10240 1606
Fragments arranged by size:
10240 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12718
AvaI C'yCGr_G
Cuts at: 1870 1943 4470 5218 6351 6530 9194 9480 10043
Size: 73 2527 748 1133 179 2664 286 563
Cuts at: 10043 1870
Size: 4545
Fragments arranged by size:
4545 2664 2527 1133 748 563 286 179
73
AvrII C'CTAG_G
Cuts at: 9982 9982
Size: 12718
BamHI G'GATC_C
Cuts at: 8736 10048 10415 8736
Size: 1312 367 11039
Fragments arranged by size:
11039 1312 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3174 3257 4194 5222 5429
Size: 325 217 627 1387 83 937 1028 207
Cuts at: 5429 5886 8861 8918 9316 10039 11494 618
Size: 457 2975 57 398 723 1455 1842
Fragments arranged by size:
2975 1842 1455 1387 1028 937 723 627
457 398 325 217 207 83 57
BanII G_rGCy'C
Cuts at: 1391 2066 2124 2607 3888 3957 4698 5778 6566
Size: 675 58 483 1281 69 741 1080 788
Cuts at: 6566 7393 7541 7785 7856 8141 8844 9312 9761
Size: 827 148 244 71 285 703 468 449
Cuts at: 9761 9942 10037 1391
Size: 181 95 4072
Fragments arranged by size:
4072 1281 1080 827 788 741 703 675
483 468 449 285 244 181 148 95
71 69 58
BbeI G_GCGC'C
Cuts at: 5433 5890 5433
Size: 457 12261
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3441 4801 4975 5129 7037 8343 928
Size: 971 1542 1360 174 154 1908 1306 5303
Fragments arranged by size:
5303 1908 1542 1360 1306 971 174 154
BbvCI CC'TCA_GC
Cuts at: 2955 8311 2955
Size: 5356 7362
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3073 3107 6481 6515 10909 10943 1903
Size: 34 1136 34 3374 34 4394 34 3678
Fragments arranged by size:
4394 3678 3374 1136 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 10610 11820 10610
Size: 1210 11508
BclI T'GATC_A
Cuts at: 1548 5331 5964 9783 1548
Size: 3783 633 3819 4483
Fragments arranged by size:
4483 3819 3783 633
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2828 3027 3244
Size: 107 122 71 631 605 1156 199 217
Cuts at: 3244 4145 6599 9128 11329 136
Size: 901 2454 2529 2201 1525
Fragments arranged by size:
2529 2454 2201 1525 1156 901 631 605
217 199 122 107 71
BglII A'GATC_T
Cuts at: 4254 6294 7248 12713 4254
Size: 2040 954 5465 4259
Fragments arranged by size:
5465 4259 2040 954
BlpI GC'TnA_GC
Cuts at: 2606 4181 4790 4848 6410 6659 6840 8043 8270
Size: 1575 609 58 1562 249 181 1203 227
Cuts at: 8270 10135 2606
Size: 1865 5189
Fragments arranged by size:
5189 1865 1575 1562 1203 609 249 227
181 58
Bme1580I G_kGCm'C
Cuts at: 1790 2186 2901 3016 3150 3216 3260 3309 4359
Size: 396 715 115 134 66 44 49 1050
Cuts at: 4359 4554 4698 4770 4819 5227 5868 6167 7393
Size: 195 144 72 49 408 641 299 1226
Cuts at: 7393 7541 7560 7598 7856 8427 8844 8866 9761
Size: 148 19 38 258 571 417 22 895
Cuts at: 9761 10779 11935 1790
Size: 1018 1156 2573
Fragments arranged by size:
2573 1226 1156 1050 1018 895 715 641
571 417 408 396 299 258 195 148
144 134 115 72 66 49 49 44
38 22 19
BmgBI CAC'GTC
Cuts at: 1318 2722 2763 5314 6190 8367 1318
Size: 1404 41 2551 876 2177 5669
Fragments arranged by size:
5669 2551 2177 1404 876 41
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2961 7171 8414
Size: 416 53 83 399 24 1544 4210 1243
Cuts at: 8414 10449 11407 442
Size: 2035 958 1753
Fragments arranged by size:
4210 2035 1753 1544 1243 958 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 12718
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8275 8275
Size: 12718
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2453 2717 3638 5159 5801 6203 6688 6830
Size: 1214 264 921 1521 642 402 485 142
Cuts at: 6830 6911 7073 8170 9653 9707 9863 11378 1239
Size: 81 162 1097 1483 54 156 1515 2579
Fragments arranged by size:
2579 1521 1515 1483 1214 1097 921 642
485 402 264 162 156 142 81 54
Bpu10I CC'TnA_GC
Cuts at: 2955 4052 6876 8311 2955
Size: 1097 2824 1435 7362
Fragments arranged by size:
7362 2824 1435 1097
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 7010 7964 8288 9161 9926 10843 11708 12006 7010
Size: 954 324 873 765 917 865 298 7722
Fragments arranged by size:
7722 954 917 873 865 765 324 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3318 3845 4895 5221 5783 7394 7823
Size: 617 1819 527 1050 326 562 1611 429
Cuts at: 7823 11381 882
Size: 3558 2219
Fragments arranged by size:
3558 2219 1819 1611 1050 617 562 527
429 326
BsaAI yAC'GTr
Cuts at: 493 1204 3192 4089 4354 4360 6268 6406 8144
Size: 711 1988 897 265 6 1908 138 1738
Cuts at: 8144 8974 9772 10509 493
Size: 830 798 737 2702
Fragments arranged by size:
2702 1988 1908 1738 897 830 798 737
711 265 138 6
BsaBI GATnn'nnATC
Cuts at: 5672 8741 9386 12712 5672
Size: 3069 645 3326 5678
Fragments arranged by size:
5678 3326 3069 645
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3345 5430 5887 8085
Size: 53 83 186 936 1812 2085 457 2198
Cuts at: 8085 10525 10907 11542 275
Size: 2440 382 635 1451
Fragments arranged by size:
2440 2198 2085 1812 1451 936 635 457
382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4002 6582 7095 11151 11549 11823 11970 12381 4002
Size: 2580 513 4056 398 274 147 411 4339
Fragments arranged by size:
4339 4056 2580 513 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2030 2060 2690 2709 2720
Size: 30 165 30 627 30 630 19 11
Cuts at: 2720 2739 3335 3365 3611 3641 3875 3905 4241
Size: 19 596 30 246 30 234 30 336
Cuts at: 4241 4271 5093 5123 6548 6578 6971 7001 7391
Size: 30 822 30 1425 30 393 30 390
Cuts at: 7391 7421 7976 8006 8513 8543 8579 8609 8675
Size: 30 555 30 507 30 36 30 66
Cuts at: 8675 8705 9053 9083 9137 9167 9206 9236 1178
Size: 30 348 30 54 30 39 30 4660
Fragments arranged by size:
4660 1425 822 630 627 596 555 507
393 390 348 336 246 234 165 66
54 39 36 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 30 30 19 19 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2714 2904 2972 3242 3245 3635 4580 4889
Size: 1528 190 68 270 3 390 945 309
Cuts at: 4889 5429 5480 6518 6827 6992 7507 8255 8258
Size: 540 51 1038 309 165 515 748 3
Cuts at: 8258 8342 8345 9074 9611 1186
Size: 84 3 729 537 4293
Fragments arranged by size:
4293 1528 1038 945 748 729 540 537
515 390 309 309 270 190 165 84
68 51 3 3 3
BseYI C'CCAG_C
Cuts at: 2894 3041 3253 4260 4267 4669 4820 4832 6690
Size: 147 212 1007 7 402 151 12 1858
Cuts at: 6690 7392 7534 7541 7777 9475 9927 11921 12417
Size: 702 142 7 236 1698 452 1994 496
Cuts at: 12417 2894
Size: 3195
Fragments arranged by size:
3195 1994 1858 1698 1007 702 496 452
402 236 212 151 147 142 12 7
7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2363 3166 3785 4334 4709 5462 5726
Size: 573 627 803 619 549 375 753 264
Cuts at: 5726 5733 5738 5897 5915 6521 6699 6851 7355
Size: 7 5 159 18 606 178 152 504
Cuts at: 7355 7598 8522 1163
Size: 243 924 5359
Fragments arranged by size:
5359 924 803 753 627 619 606 573
549 504 375 264 243 178 159 152
18 7 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2130 5161 7378 9205 10168 10929
Size: 275 186 129 3031 2217 1827 963 761
Cuts at: 10929 11957 1540
Size: 1028 2301
Fragments arranged by size:
3031 2301 2217 1827 1028 963 761 275
186 129
BsiHKAI G_wGCw'C
Cuts at: 2607 3206 3723 3957 4182 4359 5181 5868 6167
Size: 599 517 234 225 177 822 687 299
Cuts at: 6167 7608 9693 9942 10779 10864 11935 2607
Size: 1441 2085 249 837 85 1071 3390
Fragments arranged by size:
3390 2085 1441 1071 837 822 687 599
517 299 249 234 225 177 85
BsiWI C'GTAC_G
Cuts at: 3159 3159
Size: 12718
BsmI GAATG_Cn'
Cuts at: 3043 3564 3688 8232 10229 10322 3043
Size: 521 124 4544 1997 93 5439
Fragments arranged by size:
5439 4544 1997 521 124 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2050 2712 4412 7715 9056 2050
Size: 662 1700 3303 1341 5712
Fragments arranged by size:
5712 3303 1700 1341 662
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2124 2186 2607 2901 3016 3150
Size: 399 276 58 62 421 294 115 134
Cuts at: 3150 3206 3216 3260 3309 3723 3888 3957 4182
Size: 56 10 44 49 414 165 69 225
Cuts at: 4182 4359 4554 4698 4770 4819 5181 5227 5778
Size: 177 195 144 72 49 362 46 551
Cuts at: 5778 5868 6167 6566 7393 7541 7560 7598 7608
Size: 90 299 399 827 148 19 38 10
Cuts at: 7608 7785 7856 8141 8427 8844 8866 9312 9693
Size: 177 71 285 286 417 22 446 381
Cuts at: 9693 9761 9942 10037 10779 10864 11935 1391
Size: 68 181 95 742 85 1071 2174
Fragments arranged by size:
2174 1071 827 742 551 446 421 417
414 399 399 381 362 299 294 286
285 276 225 195 181 177 177 165
148 144 134 115 95 90 85 72
71 69 68 62 58 56 49 49
46 44 38 22 19 10 10
BspEI T'CCGG_A
Cuts at: 4002 6582 12381 4002
Size: 2580 5799 4339
Fragments arranged by size:
5799 4339 2580
BspHI T'CATG_A
Cuts at: 4114 5967 9013 9399 10607 12337 4114
Size: 1853 3046 386 1208 1730 4495
Fragments arranged by size:
4495 3046 1853 1730 1208 386
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2779 3269 3402 4329 4980 5275 5637 5760
Size: 1935 490 133 927 651 295 362 123
Cuts at: 5760 5877 5978 6045 6123 7290 7674 8112 8220
Size: 117 101 67 78 1167 384 438 108
Cuts at: 8220 9284 9579 10073 844
Size: 1064 295 494 3489
Fragments arranged by size:
3489 1935 1167 1064 927 651 494 490
438 384 362 295 295 133 123 117
108 101 78 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3235 6817 9105 9361 10605 1394
Size: 429 1412 3582 2288 256 1244 3507
Fragments arranged by size:
3582 3507 2288 1412 1244 429 256
BsrDI GCAATG_nn'
Cuts at: 65 1248 3091 3723 8658 8723 9103 9847 11213
Size: 1183 1843 632 4935 65 380 744 1366
Cuts at: 11213 11387 65
Size: 174 1396
Fragments arranged by size:
4935 1843 1396 1366 1183 744 632 380
174 65
BsrFI r'CCGG_y
Cuts at: 1700 1810 2618 4154 4390 5349 7182 8022 8221
Size: 110 808 1536 236 959 1833 840 199
Cuts at: 8221 8463 9204 11362 11549 1700
Size: 242 741 2158 187 2869
Fragments arranged by size:
2869 2158 1833 1536 959 840 808 741
242 236 199 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 12718
BssHII G'CGCG_C
Cuts at: 1925 4324 1925
Size: 2399 10319
BssSI C'ACGA_G
Cuts at: 4043 7559 7889 10778 11790 4043
Size: 3516 330 2889 1012 4971
Fragments arranged by size:
4971 3516 2889 1012 330
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2281 2584 5590 5863 6508 7018 7021 8212
Size: 1487 303 3006 273 645 510 3 1191
Cuts at: 8212 794
Size: 5300
Fragments arranged by size:
5300 3006 1487 1191 645 510 303 273
3
BstBI TT'CG_AA
Cuts at: 11522 11522
Size: 12718
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 12718
BstXI CCAn_nnnn'nTGG
Cuts at: 3370 3895 4504 5668 6634 7210 8950 8998 9238
Size: 525 609 1164 966 576 1740 48 240
Cuts at: 9238 9907 3370
Size: 669 6181
Fragments arranged by size:
6181 1740 1164 966 669 609 576 525
240 48
BstYI r'GATC_y
Cuts at: 1972 2989 3318 4254 4419 4914 5637 5673 6294
Size: 1017 329 936 165 495 723 36 621
Cuts at: 6294 7248 7305 7963 8736 10048 10415 10800 10817
Size: 954 57 658 773 1312 367 385 17
Cuts at: 10817 12269 12355 12367 12713 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1312 1017 954 936 773 723
658 621 495 385 367 346 329 165
86 57 36 17 12
Bsu36I CC'TnA_GG
Cuts at: 9321 9321
Size: 12718
BtgI C'CryG_G
Cuts at: 513 1065 2124 2884 3369 4503 4782 5361 5457
Size: 552 1059 760 485 1134 279 579 96
Cuts at: 5457 6539 6567 6594 6627 7410 7758 8914 8943
Size: 1082 28 27 33 783 348 1156 29
Cuts at: 8943 9406 513
Size: 463 3825
Fragments arranged by size:
3825 1156 1134 1082 1059 783 760 579
552 485 463 348 279 96 33 29
28 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3076 3471 3916 6009 8740 487
Size: 683 56 1850 395 445 2093 2731 4465
Fragments arranged by size:
4465 2731 2093 1850 683 445 395 56
BtsI GCAGTG_nn'
Cuts at: 2368 2807 3789 4664 5061 5407 5506 6259 6670
Size: 439 982 875 397 346 99 753 411
Cuts at: 6670 6904 7591 9286 9921 10234 11028 11048 2368
Size: 234 687 1695 635 313 794 20 4038
Fragments arranged by size:
4038 1695 982 875 794 753 687 635
439 411 397 346 313 234 99 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 12718
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 9406 9441 529
Size: 35 8842 35 3806
Fragments arranged by size:
8842 3806 35 35
DraI TTT'AAA
Cuts at: 10022 10022
Size: 12718
DraIII CAC_nnn'GTG
Cuts at: 5863 7179 7564 5863
Size: 1316 385 11017
Fragments arranged by size:
11017 1316 385
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2534 3494 5999 7891 11725 809
Size: 507 1218 960 2505 1892 3834 1802
Fragments arranged by size:
3834 2505 1892 1802 1218 960 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2127 2829 3360
Size: 54 1425 50 275 18 297 702 531
Cuts at: 3360 3774 4242 4701 5158 5874 6120 6600 7272
Size: 414 468 459 457 716 246 480 672
Cuts at: 7272 8637 9100 10165 11054 12683 8
Size: 1365 463 1065 889 1629 43
Fragments arranged by size:
1629 1425 1365 1065 889 716 702 672
531 480 468 463 459 457 414 297
275 246 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2127 5158 10165 1537
Size: 275 315 3031 5007 4090
Fragments arranged by size:
5007 4090 3031 315 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2177 2381 2495 4065 4115 5243 5765 6890
Size: 824 204 114 1570 50 1128 522 1125
Cuts at: 6890 8109 8266 8474 8735 9155 9176 9327 9476
Size: 1219 157 208 261 420 21 151 149
Cuts at: 9476 10648 1353
Size: 1172 3423
Fragments arranged by size:
3423 1570 1219 1172 1128 1125 824 522
420 261 208 204 157 151 149 114
50 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3653 4937 5114 8777 11305 11679
Size: 1064 138 2064 1284 177 3663 2528 374
Cuts at: 11679 11825 387
Size: 146 1280
Fragments arranged by size:
3663 2528 2064 1284 1280 1064 374 177
146 138
EcoICRI GAG'CTC
Cuts at: 2605 3955 9940 2605
Size: 1350 5985 5383
Fragments arranged by size:
5985 5383 1350
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2453 2717 3590 3638 4223 4621 4795
Size: 393 821 264 873 48 585 398 174
Cuts at: 4795 4913 4931 5159 5233 5615 5801 6203 6427
Size: 118 18 228 74 382 186 402 224
Cuts at: 6427 6688 6830 6842 6911 7073 8170 8237 9653
Size: 261 142 12 69 162 1097 67 1416
Cuts at: 9653 9707 9863 10781 11378 12165 12433 1239
Size: 54 156 918 597 787 268 1524
Fragments arranged by size:
1524 1416 1097 918 873 821 787 597
585 402 398 393 382 268 264 261
228 224 186 174 162 156 142 118
74 69 67 54 48 18 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2174 2699 3551 3776 6833 8108 8447 9332 2174
Size: 525 852 225 3057 1275 339 885 5560
Fragments arranged by size:
5560 3057 1275 885 852 525 339 225
EcoO109I rG'GnC_Cy
Cuts at: 2134 3574 3602 4694 4695 4723 5734 6961 7117
Size: 1440 28 1092 1 28 1011 1227 156
Cuts at: 7117 7538 7663 7852 7853 9325 9758 2134
Size: 421 125 189 1 1472 433 5094
Fragments arranged by size:
5094 1472 1440 1227 1092 1011 433 421
189 156 125 28 28 1 1
EcoRI G'AATT_C
Cuts at: 8960 10027 10405 8960
Size: 1067 378 11273
Fragments arranged by size:
11273 1067 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3304 9202 3304
Size: 5898 6820
FspI TGC'GCA
Cuts at: 3872 7064 11224 3872
Size: 3192 4160 5366
Fragments arranged by size:
5366 4160 3192
HaeII r_GCGC'y
Cuts at: 1678 5260 5433 5890 6916 7357 8177 8318 8779
Size: 3582 173 457 1026 441 820 141 461
Cuts at: 8779 8807 11865 1678
Size: 28 3058 2531
Fragments arranged by size:
3582 3058 2531 1026 820 461 457 441
173 141 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2030 2062 2545 2577 3080
Size: 32 135 32 458 32 483 32 503
Cuts at: 3080 3112 3335 3367 3611 3643 3890 3922 4241
Size: 32 223 32 244 32 247 32 319
Cuts at: 4241 4273 6008 6040 6179 6211 7331 7363 7391
Size: 32 1735 32 139 32 1120 32 28
Cuts at: 7391 7423 7976 8008 8513 8545 8675 8707 9287
Size: 32 553 32 505 32 130 32 580
Cuts at: 9287 9319 11359 11391 11433 11465 1373
Size: 32 2040 32 42 32 2626
Fragments arranged by size:
2626 2040 1735 1120 580 553 505 503
483 458 319 247 244 223 139 135
130 42 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 28
HincII GTy'rAC
Cuts at: 669 1657 1938 7423 7471 9202 10062 10308 669
Size: 988 281 5485 48 1731 860 246 3079
Fragments arranged by size:
5485 3079 1731 988 860 281 246 48
HindIII A'AGCT_T
Cuts at: 748 748
Size: 12718
HpaI GTT'AAC
Cuts at: 10308 10308
Size: 12718
KasI G'GCGC_C
Cuts at: 5429 5886 5429
Size: 457 12261
KpnI G_GTAC'C
Cuts at: 4198 10043 4198
Size: 5845 6873
MfeI C'AATT_G
Cuts at: 10317 10317
Size: 12718
MluI A'CGCG_T
Cuts at: 4464 12594 4464
Size: 8130 4588
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3948 4479 5319 6954 11832 12016 1305
Size: 2643 531 840 1635 4878 184 2007
Fragments arranged by size:
4878 2643 2007 1635 840 531 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3362 4244 5876 6602 7274
Size: 54 1425 343 1530 882 1632 726 672
Cuts at: 7274 10
Size: 5454
Fragments arranged by size:
5454 1632 1530 1425 882 726 672 343
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2343 2450 2904 3084 3209 4784 4892
Size: 648 1177 107 454 180 125 1575 108
Cuts at: 4892 5202 5966 6227 6278 6995 7601 8031 8699
Size: 310 764 261 51 717 606 430 668
Cuts at: 8699 8948 8954 9398 9524 10676 11035 11194 518
Size: 249 6 444 126 1152 359 159 2042
Fragments arranged by size:
2042 1575 1177 1152 764 717 668 648
606 454 444 430 359 310 261 249
180 159 126 125 108 107 51 6
MspA1I CmG'CkG
Cuts at: 1606 2109 2126 2658 3427 4135 4201 4267 4855
Size: 503 17 532 769 708 66 66 588
Cuts at: 4855 5212 5233 5293 6181 6505 6675 6683 6694
Size: 357 21 60 888 324 170 8 11
Cuts at: 6694 7060 7639 8203 8290 8581 9964 10134 10811
Size: 366 579 564 87 291 1383 170 677
Cuts at: 10811 11959 12204 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1383 1148 888 769 708 677 588
579 564 532 503 366 357 324 291
245 170 170 87 66 66 60 21
17 11 8
NaeI GCC'GGC
Cuts at: 2620 4156 7184 8024 2620
Size: 1536 3028 840 7314
Fragments arranged by size:
7314 3028 1536 840
NarI GG'CG_CC
Cuts at: 5430 5887 5430
Size: 457 12261
NcoI C'CATG_G
Cuts at: 513 1065 4503 4782 5361 6594 6627 8943 9406
Size: 552 3438 279 579 1233 33 2316 463
Cuts at: 9406 513
Size: 3825
Fragments arranged by size:
3825 3438 2316 1233 579 552 463 279
33
NdeI CA'TA_TG
Cuts at: 387 387
Size: 12718
NgoMIV G'CCGG_C
Cuts at: 2618 4154 7182 8022 2618
Size: 1536 3028 840 7314
Fragments arranged by size:
7314 3028 1536 840
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 12718
NotI GC'GGCC_GC
Cuts at: 5158 10165 5158
Size: 5007 7711
NspI r_CATG'y
Cuts at: 1132 1229 2291 2591 3661 4222 5137 5167 8578
Size: 97 1062 300 1070 561 915 30 3411
Cuts at: 8578 9618 9700 10076 11621 1132
Size: 1040 82 376 1545 2229
Fragments arranged by size:
3411 2229 1545 1070 1062 1040 915 561
376 300 97 82 30
PasI CC'CwG_GG
Cuts at: 1348 9238 1348
Size: 7890 4828
PciI A'CATG_T
Cuts at: 3657 9696 11617 3657
Size: 6039 1921 4758
Fragments arranged by size:
6039 4758 1921
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3192 3430 4240 4267 4381 4762 6040 6759
Size: 2084 238 810 27 114 381 1278 719
Cuts at: 6759 7285 7825 8077 1108
Size: 526 540 252 5749
Fragments arranged by size:
5749 2084 1278 810 719 540 526 381
252 238 114 27
PfoI T'CCnGG_A
Cuts at: 3483 3819 3894 4917 5529 5778 6888 7254 7734
Size: 336 75 1023 612 249 1110 366 480
Cuts at: 7734 9327 9879 3483
Size: 1593 552 6322
Fragments arranged by size:
6322 1593 1110 1023 612 552 480 366
336 249 75
PmeI GTTT'AAAC
Cuts at: 10022 10022
Size: 12718
PmlI CAC'GTG
Cuts at: 3192 4089 4354 4360 8144 8974 3192
Size: 897 265 6 3784 830 6936
Fragments arranged by size:
6936 3784 897 830 265 6
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2183 2215 3875 3907 9053 9085 10787 10819 12300
Size: 32 1660 32 5146 32 1702 32 1481
Cuts at: 12300 12332 2183
Size: 32 2569
Fragments arranged by size:
5146 2569 1702 1660 1481 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2134 3602 4723 6961 7117 7663 9325 2134
Size: 1468 1121 2238 156 546 1662 5527
Fragments arranged by size:
5527 2238 1662 1468 1121 546 156
PshAI GACnn'nnGTC
Cuts at: 1613 3857 8993 1613
Size: 2244 5136 5338
Fragments arranged by size:
5338 5136 2244
PsiI TTA'TAA
Cuts at: 10288 10288
Size: 12718
PspOMI G'GGCC_C
Cuts at: 4694 7389 7537 7852 8840 9757 4694
Size: 2695 148 315 988 917 7655
Fragments arranged by size:
7655 2695 988 917 315 148
PspXI vC'TCGA_Gb
Cuts at: 6530 6530
Size: 12718
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2954 2986 3632 3664 6248 6280 9695 9727 2954
Size: 32 646 32 2584 32 3415 32 5945
Fragments arranged by size:
5945 3415 2584 646 32 32 32 32
PstI C_TGCA'G
Cuts at: 830 2277 2286 2790 4713 5232 5637 5703 6362
Size: 1447 9 504 1923 519 405 66 659
Cuts at: 6362 6480 6513 6792 7014 7638 7671 7731 7977
Size: 118 33 279 222 624 33 60 246
Cuts at: 7977 8109 8181 8208 9281 10070 830
Size: 132 72 27 1073 789 3478
Fragments arranged by size:
3478 1923 1447 1073 789 659 624 519
504 405 279 246 222 132 118 72
66 60 33 33 27 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12718
PvuII CAG'CTG
Cuts at: 2658 3427 4135 4201 4267 5233 5293 6505 6675
Size: 769 708 66 66 966 60 1212 170
Cuts at: 6675 6683 7060 7639 8203 8290 2658
Size: 8 377 579 564 87 7086
Fragments arranged by size:
7086 1212 966 769 708 579 564 377
170 87 66 66 60 8
RsrII CG'GwC_CG
Cuts at: 4005 4005
Size: 12718
SacI G_AGCT'C
Cuts at: 2607 3957 9942 2607
Size: 1350 5985 5383
Fragments arranged by size:
5985 5383 1350
SacII CC_GC'GG
Cuts at: 2127 2127
Size: 12718
SalI G'TCGA_C
Cuts at: 1936 7469 9200 10060 1936
Size: 5533 1731 860 4594
Fragments arranged by size:
5533 4594 1731 860
SapI GCTCTTCn'nnn_
Cuts at: 2381 2495 5243 6890 8109 8474 8735 2381
Size: 114 2748 1647 1219 365 261 6364
Fragments arranged by size:
6364 2748 1647 1219 365 261 114
SbfI CC_TGCA'GG
Cuts at: 8109 10070 8109
Size: 1961 10757
ScaI AGT'ACT
Cuts at: 2216 3059 4064 4238 7844 10966 12678 2216
Size: 843 1005 174 3606 3122 1712 2256
Fragments arranged by size:
3606 3122 2256 1712 1005 843 174
SexAI A'CCwGG_T
Cuts at: 3438 3438
Size: 12718
SfcI C'TryA_G
Cuts at: 826 1045 2273 2282 2786 2934 4189 4630 4709
Size: 219 1228 9 504 148 1255 441 79
Cuts at: 4709 5228 5633 5699 6358 6476 6509 6545 6788
Size: 519 405 66 659 118 33 36 243
Cuts at: 6788 7010 7634 7667 7727 7973 8105 8177 8204
Size: 222 624 33 60 246 132 72 27
Cuts at: 8204 8779 9025 9277 10066 11201 11882 12073 826
Size: 575 246 252 789 1135 681 191 1471
Fragments arranged by size:
1471 1255 1228 1135 789 681 659 624
575 519 504 441 405 252 246 246
243 222 219 191 148 132 118 79
72 66 60 36 33 33 27 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 3027 3027
Size: 12718
SfoI GGC'GCC
Cuts at: 5431 5888 5431
Size: 457 12261
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12718
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 12718
SmaI CCC'GGG
Cuts at: 1872 10045 1872
Size: 8173 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6530 7025 7979 8303 9176 9194
Size: 197 926 4587 495 954 324 873 18
Cuts at: 9194 9605 9941 10822 11723 11985 820
Size: 411 336 881 901 262 1553
Fragments arranged by size:
4587 1553 954 926 901 881 873 495
411 336 324 262 197 18
SnaBI TAC'GTA
Cuts at: 493 10509 493
Size: 10016 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 12718
SphI G_CATG'C
Cuts at: 1132 2291 2591 5137 8578 10076 1132
Size: 1159 300 2546 3441 1498 3774
Fragments arranged by size:
3774 3441 2546 1498 1159 300
SspI AAT'ATT
Cuts at: 5 52 10642 5
Size: 47 10590 2081
Fragments arranged by size:
10590 2081 47
StuI AGG'CCT
Cuts at: 1517 3287 4715 6575 6905 8099 1517
Size: 1770 1428 1860 330 1194 6136
Fragments arranged by size:
6136 1860 1770 1428 1194 330
StyI C'CwwG_G
Cuts at: 513 1065 2994 3270 4503 4782 5361 5436 6393
Size: 552 1929 276 1233 279 579 75 957
Cuts at: 6393 6594 6603 6627 6957 7824 8943 9406 9982
Size: 201 9 24 330 867 1119 463 576
Cuts at: 9982 10157 513
Size: 175 3074
Fragments arranged by size:
3074 1929 1233 1119 957 867 579 576
552 463 330 279 276 201 175 75
24 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3857 3962 4022 5374 5496 7190 8849
Size: 1255 2277 105 60 1352 122 1694 1659
Cuts at: 8849 9761 10745 10762 10915 11100 11545 325
Size: 912 984 17 153 185 445 1498
Fragments arranged by size:
2277 1694 1659 1498 1352 1255 984 912
445 185 153 122 105 60 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2214 2772 3057
Size: 274 80 33 51 1443 237 558 285
Cuts at: 3057 4062 4236 7842 9711 10964 12676 96
Size: 1005 174 3606 1869 1253 1712 138
Fragments arranged by size:
3606 1869 1712 1443 1253 1005 558 285
274 237 174 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2709 2741 4760 4792 6548 6580 6971 7003 7730
Size: 32 2019 32 1756 32 391 32 727
Cuts at: 7730 7762 8838 8870 12678 12710 2709
Size: 32 1076 32 3808 32 2717
Fragments arranged by size:
3808 2717 2019 1756 1076 727 391 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2042 7405 9299 9661 1316
Size: 726 5363 1894 362 4373
Fragments arranged by size:
5363 4373 1894 726 362
XbaI T'CTAG_A
Cuts at: 10054 10054
Size: 12718
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3355 3844 4510 4759 5074 5113 6964 7282 7286
Size: 489 666 249 315 39 1851 318 4
Cuts at: 7286 7411 7489 7708 7765 8002 8701 8827 9904
Size: 125 78 219 57 237 699 126 1077
Cuts at: 9904 3355
Size: 6169
Fragments arranged by size:
6169 1851 1077 699 666 489 318 315
249 237 219 126 125 78 57 39
4
XhoI C'TCGA_G
Cuts at: 1943 6530 9194 1943
Size: 4587 2664 5467
Fragments arranged by size:
5467 4587 2664
XmaI C'CCGG_G
Cuts at: 1870 10043 1870
Size: 8173 4545
XmnI GAAnn'nnTTC
Cuts at: 4926 7250 7316 10847 4926
Size: 2324 66 3531 6797
Fragments arranged by size:
6797 3531 2324 66
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8086 10526 276
Size: 53 83 186 936 6552 2440 2468
Fragments arranged by size:
6552 2468 2440 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BfrBI BstZ17I EcoRV FseI FspAI NruI
NsiI PacI SanDI SrfI SwaI