Restriction map for
pFN21AE3825
(Circular) (Six-base) MAPSORT of: pFN21AE3825.seq Check: 3491 from: 1 to: 12535
pFN21AE3825 12535 bp
With 182 enzymes: SgfI *
August 14, 2013 14:06 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5797 10345 278
Size: 53 83 186 936 4261 4548 2468
Fragments arranged by size:
4548 4261 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4879 4936 5546 9878 1937
Size: 2942 57 610 4332 4594
Fragments arranged by size:
4594 4332 2942 610 57
Acc65I G'GTAC_C
Cuts at: 7204 9856 7204
Size: 2652 9883
AclI AA'CG_TT
Cuts at: 1563 3881 10662 11035 1563
Size: 2318 6781 373 3063
Fragments arranged by size:
6781 3063 2318 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3425 3693 6519 7991 8934 9165 9203 9430
Size: 1793 268 2826 1472 943 231 38 227
Cuts at: 9430 10598 11982 12250 1632
Size: 1168 1384 268 1917
Fragments arranged by size:
2826 1917 1793 1472 1384 1168 943 268
268 231 227 38
AfeI AGC'GCT
Cuts at: 3086 3086
Size: 12535
AflII C'TTAA_G
Cuts at: 820 1017 9752 820
Size: 197 8735 3603
Fragments arranged by size:
8735 3603 197
AflIII A'CryG_T
Cuts at: 1203 2144 4286 5638 8258 9545 9737 11434 12411
Size: 941 2142 1352 2620 1287 192 1697 977
Cuts at: 12411 1203
Size: 1327
Fragments arranged by size:
2620 2142 1697 1352 1327 1287 977 941
192
AgeI A'CCGG_T
Cuts at: 11366 11366
Size: 12535
AhdI GACnn_n'nnGTC
Cuts at: 11264 11264
Size: 12535
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 12535
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 7779 8475 1184
Size: 6595 696 5244
Fragments arranged by size:
6595 5244 696
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2460 2492 2460
Size: 32 12503
AlwNI CAG_nnn'CTG
Cuts at: 5815 6263 6488 7772 8014 9200 10278 11850 5815
Size: 448 225 1284 242 1186 1078 1572 6500
Fragments arranged by size:
6500 1572 1284 1186 1078 448 242 225
ApaI G_GGCC'C
Cuts at: 6583 6583
Size: 12535
ApaLI G'TGCA_C
Cuts at: 5019 10592 11748 5019
Size: 5573 1156 5806
Fragments arranged by size:
5806 5573 1156
ApoI r'AATT_y
Cuts at: 1493 2181 2559 3172 3242 4172 4430 5188 5990
Size: 688 378 613 70 930 258 758 802
Cuts at: 5990 8108 8489 8555 8751 8981 9030 9120 9235
Size: 2118 381 66 196 230 49 90 115
Cuts at: 9235 9844 10070 10222 12331 1493
Size: 609 226 152 2109 1697
Fragments arranged by size:
2118 2109 1697 930 802 758 688 613
609 381 378 258 230 226 196 152
115 90 70 66 49
AseI AT'TA_AT
Cuts at: 160 1032 11089 160
Size: 872 10057 1606
Fragments arranged by size:
10057 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12535
AvaI C'yCGr_G
Cuts at: 1870 1943 3405 4197 4515 9860 1870
Size: 73 1462 792 318 5345 4545
Fragments arranged by size:
5345 4545 1462 792 318 73
BamHI G'GATC_C
Cuts at: 4964 9865 10232 4964
Size: 4901 367 7267
Fragments arranged by size:
7267 4901 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3553 5202 7204 8273 9114
Size: 325 217 627 1766 1649 2002 1069 841
Cuts at: 9114 9856 11311 618
Size: 742 1455 1842
Fragments arranged by size:
2002 1842 1766 1649 1455 1069 841 742
627 325 217
BanII G_rGCy'C
Cuts at: 1391 2623 2794 3534 4198 5839 6583 6593 7029
Size: 1232 171 740 664 1641 744 10 436
Cuts at: 7029 7419 7614 8182 9854 1391
Size: 390 195 568 1672 4072
Fragments arranged by size:
4072 1672 1641 1232 744 740 664 568
436 390 195 171 10
BbeI G_GCGC'C
Cuts at: 8277 9118 8277
Size: 841 11694
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3325 4679 5355 9051 9588 928
Size: 971 1426 1354 676 3696 537 3875
Fragments arranged by size:
3875 3696 1426 1354 971 676 537
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 10726 10760 1903
Size: 34 8789 34 3678
Fragments arranged by size:
8789 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5524 10427 11637 5524
Size: 4903 1210 6422
Fragments arranged by size:
6422 4903 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 12535
BfrBI ATG'CAT
Cuts at: 2100 8769 9367 2100
Size: 6669 598 5268
Fragments arranged by size:
6669 5268 598
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 11146 136
Size: 107 122 71 631 605 9474 1525
Fragments arranged by size:
9474 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3364 5422 12530 3364
Size: 2058 7108 3369
Fragments arranged by size:
7108 3369 2058
BlpI GC'TnA_GC
Cuts at: 7023 9952 7023
Size: 2929 9606
Bme1580I G_kGCm'C
Cuts at: 1790 5023 6583 10596 11752 1790
Size: 3233 1560 4013 1156 2573
Fragments arranged by size:
4013 3233 2573 1560 1156
BmgBI CAC'GTC
Cuts at: 1318 8309 1318
Size: 6991 5544
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3217 10266 11224
Size: 416 53 83 399 24 1800 7049 958
Cuts at: 11224 442
Size: 1753
Fragments arranged by size:
7049 1800 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3851 9803 1056
Size: 2795 5952 3788
Fragments arranged by size:
5952 3788 2795
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2187 3432 4964 7233 8385 11195 1239
Size: 948 1245 1532 2269 1152 2810 2579
Fragments arranged by size:
2810 2579 2269 1532 1245 1152 948
Bpu10I CC'TnA_GC
Cuts at: 7050 9531 7050
Size: 2481 10054
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2526 3309 5984 6124 6604 8263 8372 8501 9241
Size: 783 2675 140 480 1659 109 129 740
Cuts at: 9241 9272 10660 11525 11823 2526
Size: 31 1388 865 298 3238
Fragments arranged by size:
3238 2675 1659 1388 865 783 740 480
298 140 129 109 31
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5435 11198 882
Size: 617 3936 5763 2219
Fragments arranged by size:
5763 3936 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 8414 9548 10326 493
Size: 711 7210 1134 778 2702
Fragments arranged by size:
7210 2702 1134 778 711
BsaBI GATnn'nnATC
Cuts at: 7086 12529 7086
Size: 5443 7092
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5794 7129 7273 8274
Size: 53 83 186 936 4261 1335 144 1001
Cuts at: 8274 9115 10342 10724 11359 275
Size: 841 1227 382 635 1451
Fragments arranged by size:
4261 1451 1335 1227 1001 936 841 635
382 186 144 83 53
BsaWI w'CCGG_w
Cuts at: 2875 3060 3544 4135 10968 11366 11640 11787 12198
Size: 185 484 591 6833 398 274 147 411
Cuts at: 12198 2875
Size: 3212
Fragments arranged by size:
6833 3212 591 484 411 398 274 185
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2134 2164 2776 2806 4557
Size: 30 165 30 731 30 612 30 1751
Cuts at: 4557 4587 5826 5856 6252 6282 1178
Size: 30 1239 30 396 30 7431
Fragments arranged by size:
7431 1751 1239 731 612 396 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2142 2720 6127 6759 7124 9791 1186
Size: 956 578 3407 632 365 2667 3930
Fragments arranged by size:
3930 3407 2667 956 632 578 365
BseYI C'CCAG_C
Cuts at: 2674 6575 9013 9535 11738 12234 2674
Size: 3901 2438 522 2203 496 2975
Fragments arranged by size:
3901 2975 2438 2203 522 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3282 5472 8684 1163
Size: 573 1546 2190 3212 5014
Fragments arranged by size:
5014 3212 2190 1546 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 9985 10746 11774 1540
Size: 275 186 7984 761 1028 2301
Fragments arranged by size:
7984 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2203 2623 2794 4198 5023 5839 6593 6758 6784
Size: 420 171 1404 825 816 754 165 26
Cuts at: 6784 7029 7419 10596 10681 11752 2203
Size: 245 390 3177 85 1071 2986
Fragments arranged by size:
3177 2986 1404 1071 825 816 754 420
390 245 171 165 85 26
BsmI GAATG_Cn'
Cuts at: 5410 7768 8769 9614 10046 10139 5410
Size: 2358 1001 845 432 93 7806
Fragments arranged by size:
7806 2358 1001 845 432 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5785 7120 8390 5785
Size: 1335 1270 9930
Fragments arranged by size:
9930 1335 1270
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2203 2623 2794 3534 4198 5023 5839
Size: 399 413 420 171 740 664 825 816
Cuts at: 5839 6583 6593 6758 6784 7029 7419 7614 8182
Size: 744 10 165 26 245 390 195 568
Cuts at: 8182 9854 10596 10681 11752 1391
Size: 1672 742 85 1071 2174
Fragments arranged by size:
2174 1672 1071 825 816 744 742 740
664 568 420 413 399 390 245 195
171 165 85 26 10
BspEI T'CCGG_A
Cuts at: 3060 3544 4135 12198 3060
Size: 484 591 8063 3397
Fragments arranged by size:
8063 3397 591 484
BspHI T'CATG_A
Cuts at: 8935 9783 10424 12154 8935
Size: 848 641 1730 9316
Fragments arranged by size:
9316 1730 848 641
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3878 7216 7761 9678 9890 844
Size: 3034 3338 545 1917 212 3489
Fragments arranged by size:
3489 3338 3034 1917 545 212
BsrBI CCG'CTC
Cuts at: 1394 1823 7161 10422 1394
Size: 429 5338 3261 3507
Fragments arranged by size:
5338 3507 3261 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2027 2930 5398 6868 7582 7978 11030
Size: 1183 779 903 2468 1470 714 396 3052
Cuts at: 11030 11204 65
Size: 174 1396
Fragments arranged by size:
3052 2468 1470 1396 1183 903 779 714
396 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 11179 11366 1700
Size: 110 9369 187 2869
Fragments arranged by size:
9369 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 7543 7629 8261 96
Size: 7447 86 632 4370
Fragments arranged by size:
7447 4370 632 86
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 12535
BssSI C'ACGA_G
Cuts at: 2400 4560 5469 5838 7491 8874 10595 11607 2400
Size: 2160 909 369 1653 1383 1721 1012 3328
Fragments arranged by size:
3328 2160 1721 1653 1383 1012 909 369
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3610 3638 5261 7772 794
Size: 2816 28 1623 2511 5557
Fragments arranged by size:
5557 2816 2511 1623 28
BstBI TT'CG_AA
Cuts at: 3115 3649 4900 5167 5706 11339 3115
Size: 534 1251 267 539 5633 4311
Fragments arranged by size:
5633 4311 1251 539 534 267
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 12535
BstXI CCAn_nnnn'nTGG
Cuts at: 3456 3990 5425 8069 3456
Size: 534 1435 2644 7922
Fragments arranged by size:
7922 2644 1435 534
BstYI r'GATC_y
Cuts at: 1972 3035 3364 3541 4145 4964 5422 9283 9865
Size: 1063 329 177 604 819 458 3861 582
Cuts at: 9865 10232 10617 10634 12086 12172 12184 12530 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
3861 1977 1452 1063 819 604 582 458
385 367 346 329 177 86 17 12
BstZ17I GTA'TAC
Cuts at: 5547 5547
Size: 12535
Bsu36I CC'TnA_GG
Cuts at: 7956 9566 7956
Size: 1610 10925
BtgI C'CryG_G
Cuts at: 513 1065 2004 6444 513
Size: 552 939 4440 6604
Fragments arranged by size:
6604 4440 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2641 487
Size: 683 56 1415 10381
Fragments arranged by size:
10381 1415 683 56
BtsI GCAGTG_nn'
Cuts at: 2802 3741 4750 6721 8350 10051 10845 10865 2802
Size: 939 1009 1971 1629 1701 794 20 4472
Fragments arranged by size:
4472 1971 1701 1629 1009 939 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 12535
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3714 3749 9277 9312 529
Size: 35 3150 35 5528 35 3752
Fragments arranged by size:
5528 3752 3150 35 35 35
DraI TTT'AAA
Cuts at: 4540 4843 5379 8818 9084 9234 9839 4540
Size: 303 536 3439 266 150 605 7236
Fragments arranged by size:
7236 3439 605 536 303 266 150
DraIII CAC_nnn'GTG
Cuts at: 5027 8879 5027
Size: 3852 8683
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 11542 809
Size: 507 10226 1802
Fragments arranged by size:
10226 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3778 5338 6177
Size: 54 1425 50 275 18 1948 1560 839
Cuts at: 6177 8760 9982 10871 12500 8
Size: 2583 1222 889 1629 43
Fragments arranged by size:
2583 1948 1629 1560 1425 1222 889 839
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 9982 1537
Size: 275 8170 4090
Fragments arranged by size:
8170 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3909 6538 7245 7920 8958 10465 1353
Size: 2556 2629 707 675 1038 1507 3423
Fragments arranged by size:
3423 2629 2556 1507 1038 707 675
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 11122 11496 11642 387
Size: 1064 138 9533 374 146 1280
Fragments arranged by size:
9533 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2621 2792 4196 5837 6591 7027 7417 2621
Size: 171 1404 1641 754 436 390 7739
Fragments arranged by size:
7739 1641 1404 754 436 390 171
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2187 3425 3432 3693 4964 6519 7233
Size: 393 555 1238 7 261 1271 1555 714
Cuts at: 7233 7991 8385 8934 9165 9203 9430 10598 11195
Size: 758 394 549 231 38 227 1168 597
Cuts at: 11195 11982 12250 1239
Size: 787 268 1524
Fragments arranged by size:
1555 1524 1271 1238 1168 787 758 714
597 555 549 394 393 268 261 231
227 38 7
EcoO109I rG'GnC_Cy
Cuts at: 3890 4250 6398 6579 6580 7401 8519 3890
Size: 360 2148 181 1 821 1118 7906
Fragments arranged by size:
7906 2148 1118 821 360 181 1
EcoRI G'AATT_C
Cuts at: 4172 8108 8555 9844 10222 4172
Size: 3936 447 1289 378 6485
Fragments arranged by size:
6485 3936 1289 447 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3653 4781 5795 6930 7756 8021 8061 3653
Size: 1128 1014 1135 826 265 40 8127
Fragments arranged by size:
8127 1135 1128 1014 826 265 40
FspI TGC'GCA
Cuts at: 11041 11041
Size: 12535
HaeII r_GCGC'y
Cuts at: 1678 3088 8277 9118 9796 11682 1678
Size: 1410 5189 841 678 1886 2531
Fragments arranged by size:
5189 2531 1886 1410 841 678
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2383 2415 2699 2731 6104
Size: 32 135 32 811 32 284 32 3373
Cuts at: 6104 6136 6252 6284 7363 7395 7443 7475 7515
Size: 32 116 32 1079 32 48 32 40
Cuts at: 7515 7547 8456 8488 8597 8629 8930 8960 8962
Size: 32 909 32 109 32 301 30 2
Cuts at: 8962 8992 11176 11208 11250 11282 1373
Size: 30 2184 32 42 32 2626
Fragments arranged by size:
3373 2626 2184 1079 909 811 301 284
135 116 109 48 42 40 32 32
32 32 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2671 2835 2977 3879 4229 4817
Size: 988 281 733 164 142 902 350 588
Cuts at: 4817 4880 5586 6837 9879 10125 669
Size: 63 706 1251 3042 246 3079
Fragments arranged by size:
3079 3042 1251 988 902 733 706 588
350 281 246 164 142 63
HindIII A'AGCT_T
Cuts at: 748 3012 6002 8916 8956 748
Size: 2264 2990 2914 40 4327
Fragments arranged by size:
4327 2990 2914 2264 40
HpaI GTT'AAC
Cuts at: 10125 10125
Size: 12535
KasI G'GCGC_C
Cuts at: 8273 9114 8273
Size: 841 11694
KpnI G_GTAC'C
Cuts at: 7208 9860 7208
Size: 2652 9883
MfeI C'AATT_G
Cuts at: 2838 2919 3147 6279 7559 10134 2838
Size: 81 228 3132 1280 2575 5239
Fragments arranged by size:
5239 3132 2575 1280 228 81
MluI A'CGCG_T
Cuts at: 12411 12411
Size: 12535
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2883 4992 11649 11833 1305
Size: 1578 2109 6657 184 2007
Fragments arranged by size:
6657 2109 2007 1578 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3780 6179 8762 10
Size: 54 1425 343 1948 2399 2583 3783
Fragments arranged by size:
3783 2583 2399 1948 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2761 4285 4950 5208 5391 5469 5556
Size: 648 1595 1524 665 258 183 78 87
Cuts at: 5556 7625 9831 10493 10852 11011 518
Size: 2069 2206 662 359 159 2042
Fragments arranged by size:
2206 2069 2042 1595 1524 665 662 648
359 258 183 159 87 78
MspA1I CmG'CkG
Cuts at: 1606 5285 6362 9278 9951 10628 11776 12021 1606
Size: 3679 1077 2916 673 677 1148 245 2120
Fragments arranged by size:
3679 2916 2120 1148 1077 677 673 245
NarI GG'CG_CC
Cuts at: 8274 9115 8274
Size: 841 11694
NcoI C'CATG_G
Cuts at: 513 1065 2004 6444 513
Size: 552 939 4440 6604
Fragments arranged by size:
6604 4440 939 552
NdeI CA'TA_TG
Cuts at: 387 4729 387
Size: 4342 8193
NheI G'CTAG_C
Cuts at: 1052 3847 9799 1052
Size: 2795 5952 3788
Fragments arranged by size:
5952 3788 2795
NotI GC'GGCC_GC
Cuts at: 9982 9982
Size: 12535
NsiI A_TGCA'T
Cuts at: 2102 8771 9369 2102
Size: 6669 598 5268
Fragments arranged by size:
6669 5268 598
NspI r_CATG'y
Cuts at: 1132 1229 2074 2104 2148 2766 3312 4290 4558
Size: 97 845 30 44 618 546 978 268
Cuts at: 4558 5642 7700 8262 8462 9595 9741 9893 11438
Size: 1084 2058 562 200 1133 146 152 1545
Cuts at: 11438 1132
Size: 2229
Fragments arranged by size:
2229 2058 1545 1133 1084 978 845 618
562 546 268 200 152 146 97 44
30
PasI CC'CwG_GG
Cuts at: 1348 4420 7397 1348
Size: 3072 2977 6486
Fragments arranged by size:
6486 3072 2977
PciI A'CATG_T
Cuts at: 2144 4286 5638 8258 9737 11434 2144
Size: 2142 1352 2620 1479 1697 3245
Fragments arranged by size:
3245 2620 2142 1697 1479 1352
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3787 1108
Size: 2679 9856
PfoI T'CCnGG_A
Cuts at: 3753 5830 8650 3753
Size: 2077 2820 7638
Fragments arranged by size:
7638 2820 2077
PmeI GTTT'AAAC
Cuts at: 9839 9839
Size: 12535
PmlI CAC'GTG
Cuts at: 8414 9548 8414
Size: 1134 11401
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4498 4530 8391 8423 10604 10636 12117 12149 4498
Size: 32 3861 32 2181 32 1481 32 4884
Fragments arranged by size:
4884 3861 2181 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3890 4250 7401 8519 3890
Size: 360 3151 1118 7906
Fragments arranged by size:
7906 3151 1118 360
PshAI GACnn'nnGTC
Cuts at: 1613 5565 6957 1613
Size: 3952 1392 7191
Fragments arranged by size:
7191 3952 1392
PsiI TTA'TAA
Cuts at: 10105 10105
Size: 12535
PspOMI G'GGCC_C
Cuts at: 6579 6579
Size: 12535
PspXI vC'TCGA_Gb
Cuts at: 4197 4197
Size: 12535
PstI C_TGCA'G
Cuts at: 830 2809 4411 5567 6133 7758 9887 830
Size: 1979 1602 1156 566 1625 2129 3478
Fragments arranged by size:
3478 2129 1979 1625 1602 1156 566
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12535
PvuII CAG'CTG
Cuts at: 5285 9278 5285
Size: 3993 8542
SacI G_AGCT'C
Cuts at: 2623 2794 4198 5839 6593 7029 7419 2623
Size: 171 1404 1641 754 436 390 7739
Fragments arranged by size:
7739 1641 1404 754 436 390 171
SalI G'TCGA_C
Cuts at: 1936 4878 9877 1936
Size: 2942 4999 4594
Fragments arranged by size:
4999 4594 2942
SanDI GG'GwC_CC
Cuts at: 7401 8519 7401
Size: 1118 11417
SapI GCTCTTCn'nnn_
Cuts at: 6538 6538
Size: 12535
SbfI CC_TGCA'GG
Cuts at: 9887 9887
Size: 12535
ScaI AGT'ACT
Cuts at: 7012 10783 12495 7012
Size: 3771 1712 7052
Fragments arranged by size:
7052 3771 1712
SexAI A'CCwGG_T
Cuts at: 2770 4360 5023 6808 7348 2770
Size: 1590 663 1785 540 7957
Fragments arranged by size:
7957 1785 1590 663 540
SfcI C'TryA_G
Cuts at: 826 1045 2042 2805 3039 4266 4407 5082 5563
Size: 219 997 763 234 1227 141 675 481
Cuts at: 5563 6129 6870 7689 7754 8922 9883 11018 11699
Size: 566 741 819 65 1168 961 1135 681
Cuts at: 11699 11890 826
Size: 191 1471
Fragments arranged by size:
1471 1227 1168 1135 997 961 819 763
741 681 675 566 481 234 219 191
141 65
SfoI GGC'GCC
Cuts at: 8275 9116 8275
Size: 841 11694
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12535
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 12535
SmaI CCC'GGG
Cuts at: 1872 9862 1872
Size: 7990 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2541 3324 3405 4197 4515 5999
Size: 197 926 598 783 81 792 318 1484
Cuts at: 5999 6139 6619 8278 8387 8480 9256 9287 9752
Size: 140 480 1659 109 93 776 31 465
Cuts at: 9752 10639 11540 11802 820
Size: 887 901 262 1553
Fragments arranged by size:
1659 1553 1484 926 901 887 792 783
776 598 480 465 318 262 197 140
109 93 81 31
SnaBI TAC'GTA
Cuts at: 493 10326 493
Size: 9833 2702
SpeI A'CTAG_T
Cuts at: 152 3730 9175 152
Size: 3578 5445 3512
Fragments arranged by size:
5445 3578 3512
SphI G_CATG'C
Cuts at: 1132 2104 2766 4558 9893 1132
Size: 972 662 1792 5335 3774
Fragments arranged by size:
5335 3774 1792 972 662
SspI AAT'ATT
Cuts at: 5 52 4344 6974 8139 9767 10459 5
Size: 47 4292 2630 1165 1628 692 2081
Fragments arranged by size:
4292 2630 2081 1628 1165 692 47
StuI AGG'CCT
Cuts at: 1517 5853 1517
Size: 4336 8199
StyI C'CwwG_G
Cuts at: 513 1065 2004 4053 6444 7711 8746 9974 513
Size: 552 939 2049 2391 1267 1035 1228 3074
Fragments arranged by size:
3074 2391 2049 1267 1228 1035 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2664 3571 5160 5425 6055 6767 8822
Size: 1255 1084 907 1589 265 630 712 2055
Cuts at: 8822 10562 10579 10732 10917 11362 325
Size: 1740 17 153 185 445 1498
Fragments arranged by size:
2055 1740 1589 1498 1255 1084 907 712
630 445 265 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2396 4696 4915
Size: 274 80 33 51 1443 419 2300 219
Cuts at: 4915 7010 7436 7543 7629 8261 8897 10781 12493
Size: 2095 426 107 86 632 636 1884 1712
Cuts at: 12493 96
Size: 138
Fragments arranged by size:
2300 2095 1884 1712 1443 636 632 426
419 274 219 138 107 86 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2776 2808 8646 8678 12495 12527 2776
Size: 32 5838 32 3817 32 2784
Fragments arranged by size:
5838 3817 2784 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 6832 7350 1316
Size: 5516 518 6501
Fragments arranged by size:
6501 5516 518
XbaI T'CTAG_A
Cuts at: 7381 9871 7381
Size: 2490 10045
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 7015 2011
Size: 5004 7531
XhoI C'TCGA_G
Cuts at: 1943 3405 4197 4515 1943
Size: 1462 792 318 9963
Fragments arranged by size:
9963 1462 792 318
XmaI C'CCGG_G
Cuts at: 1870 9860 1870
Size: 7990 4545
XmnI GAAnn'nnTTC
Cuts at: 7834 8985 9614 10664 7834
Size: 1151 629 1050 9705
Fragments arranged by size:
9705 1151 1050 629
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5795 10343 276
Size: 53 83 186 936 4261 4548 2468
Fragments arranged by size:
4548 4261 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbvCI BplI BsiWI EcoNI
EcoRV FseI FspAI NaeI NgoMIV NruI PacI PsrI
RsrII SacII SfiI SrfI SwaI