Restriction map for
pFN21AE3824
(Circular) (Six-base) MAPSORT of: pFN21AE3824.seq Check: 7011 from: 1 to: 12367
pFN21AE3824 12367 bp
With 182 enzymes: SgfI *
March 28, 2012 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 7484 7484
Size: 12367
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 10177 278
Size: 53 83 186 936 8641 2468
Fragments arranged by size:
8641 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7230 9710 1937
Size: 5293 2480 4594
Fragments arranged by size:
5293 4594 2480
Acc65I G'GTAC_C
Cuts at: 9688 9688
Size: 12367
AclI AA'CG_TT
Cuts at: 1563 2748 10494 10867 1563
Size: 1185 7746 373 3063
Fragments arranged by size:
7746 3063 1185 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2634 4163 4493 5238 5594 6213 6924 6929
Size: 1002 1529 330 745 356 619 711 5
Cuts at: 6929 8130 9504 10430 11814 12082 1632
Size: 1201 1374 926 1384 268 1917
Fragments arranged by size:
1917 1529 1384 1374 1201 1002 926 745
711 619 356 330 268 5
AfeI AGC'GCT
Cuts at: 7352 9128 7352
Size: 1776 10591
AflII C'TTAA_G
Cuts at: 820 1017 8452 820
Size: 197 7435 4735
Fragments arranged by size:
7435 4735 197
AflIII A'CryG_T
Cuts at: 1203 2573 8057 11266 12243 1203
Size: 1370 5484 3209 977 1327
Fragments arranged by size:
5484 3209 1370 1327 977
AgeI A'CCGG_T
Cuts at: 11198 11198
Size: 12367
AhdI GACnn_n'nnGTC
Cuts at: 4418 11096 4418
Size: 6678 5689
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 12367
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 12367
AlwNI CAG_nnn'CTG
Cuts at: 8593 10110 11682 8593
Size: 1517 1572 9278
Fragments arranged by size:
9278 1572 1517
ApaLI G'TGCA_C
Cuts at: 5313 6022 10424 11580 5313
Size: 709 4402 1156 6100
Fragments arranged by size:
6100 4402 1156 709
ApoI r'AATT_y
Cuts at: 1493 2128 2394 2433 2552 2684 2950 3036 3729
Size: 635 266 39 119 132 266 86 693
Cuts at: 3729 3760 5802 6341 6709 7067 7454 7700 9066
Size: 31 2042 539 368 358 387 246 1366
Cuts at: 9066 9087 9676 9902 10054 12163 1493
Size: 21 589 226 152 2109 1697
Fragments arranged by size:
2109 2042 1697 1366 693 635 589 539
387 368 358 266 266 246 226 152
132 119 86 39 31 21
AseI AT'TA_AT
Cuts at: 160 1032 2730 4149 6177 6389 6498 10921 160
Size: 872 1698 1419 2028 212 109 4423 1606
Fragments arranged by size:
4423 2028 1698 1606 1419 872 212 109
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12367
AvaI C'yCGr_G
Cuts at: 1870 1943 7461 8087 9692 1870
Size: 73 5518 626 1605 4545
Fragments arranged by size:
5518 4545 1605 626 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 7186 7219 7186
Size: 33 12334
BamHI G'GATC_C
Cuts at: 9697 10064 9697
Size: 367 12000
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5441 7824 9688 11143 618
Size: 325 217 627 3654 2383 1864 1455 1842
Fragments arranged by size:
3654 2383 1864 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4806 9686 1391
Size: 3415 4880 4072
Fragments arranged by size:
4880 4072 3415
BbeI G_GCGC'C
Cuts at: 7828 7828
Size: 12367
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3206 4449 5819 5850 8588 8736 8883
Size: 971 1307 1243 1370 31 2738 148 147
Cuts at: 8883 9295 928
Size: 412 4000
Fragments arranged by size:
4000 2738 1370 1307 1243 971 412 148
147 31
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 10558 10592 1903
Size: 34 8621 34 3678
Fragments arranged by size:
8621 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3165 9652 10259 11469 3165
Size: 6487 607 1210 4063
Fragments arranged by size:
6487 4063 1210 607
BclI T'GATC_A
Cuts at: 1548 2238 3400 5581 7162 7795 8130 8464 1548
Size: 690 1162 2181 1581 633 335 334 5451
Fragments arranged by size:
5451 2181 1581 1162 690 633 335 334
BfrBI ATG'CAT
Cuts at: 7307 8002 8222 7307
Size: 695 220 11452
Fragments arranged by size:
11452 695 220
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 10978 136
Size: 107 122 71 631 605 9306 1525
Fragments arranged by size:
9306 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4375 7170 9232 12362 4375
Size: 2795 2062 3130 4380
Fragments arranged by size:
4380 3130 2795 2062
BlpI GC'TnA_GC
Cuts at: 4054 5670 9784 4054
Size: 1616 4114 6637
Fragments arranged by size:
6637 4114 1616
Bme1580I G_kGCm'C
Cuts at: 1790 3598 5317 5608 6026 6996 8327 10428 11584
Size: 1808 1719 291 418 970 1331 2101 1156
Cuts at: 11584 1790
Size: 2573
Fragments arranged by size:
2573 2101 1808 1719 1331 1156 970 418
291
BmgBI CAC'GTC
Cuts at: 1318 2576 1318
Size: 1258 11109
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 7423 9332 10098
Size: 416 53 83 399 24 6006 1909 766
Cuts at: 10098 11056 442
Size: 958 1753
Fragments arranged by size:
6006 1909 1753 958 766 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 12367
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3789 3789
Size: 12367
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2622 5046 8339 9441 11027 1239
Size: 1383 2424 3293 1102 1586 2579
Fragments arranged by size:
3293 2579 2424 1586 1383 1102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2581 3488 8479 8515 10492 11357 11655 2581
Size: 907 4991 36 1977 865 298 3293
Fragments arranged by size:
4991 3293 1977 907 865 298 36
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3894 4725 9336 11030 882
Size: 617 2395 831 4611 1694 2219
Fragments arranged by size:
4611 2395 2219 1694 831 617
BsaAI yAC'GTr
Cuts at: 493 1204 10158 493
Size: 711 8954 2702
Fragments arranged by size:
8954 2702 711
BsaBI GATnn'nnATC
Cuts at: 7909 12361 7909
Size: 4452 7915
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7825 8521 10174 10556
Size: 53 83 186 936 6292 696 1653 382
Cuts at: 10556 11191 275
Size: 635 1451
Fragments arranged by size:
6292 1653 1451 936 696 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2029 6472 10800 11198 11472 11619 12030 2029
Size: 4443 4328 398 274 147 411 2366
Fragments arranged by size:
4443 4328 2366 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3125 3155 3342 3372 3608
Size: 30 165 30 1722 30 187 30 236
Cuts at: 3608 3638 4703 4733 4945 4975 5112 5142 1178
Size: 30 1065 30 212 30 137 30 8403
Fragments arranged by size:
8403 1722 1065 236 212 187 165 137
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3146 4017 4370 8073 8754 9446 9449 1186
Size: 1960 871 353 3703 681 692 3 4104
Fragments arranged by size:
4104 3703 1960 871 692 681 353 3
BseYI C'CCAG_C
Cuts at: 3934 8925 9565 11570 12066 3934
Size: 4991 640 2005 496 4235
Fragments arranged by size:
4991 4235 2005 640 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 8352 1163
Size: 573 6616 5178
Fragments arranged by size:
6616 5178 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2034 9817 10578 11606 1540
Size: 275 186 33 7783 761 1028 2301
Fragments arranged by size:
7783 2301 1028 761 275 186 33
BsiHKAI G_wGCw'C
Cuts at: 4027 4806 5317 6026 6095 7611 9613 10428 10513
Size: 779 511 709 69 1516 2002 815 85
Cuts at: 10513 11584 4027
Size: 1071 4810
Fragments arranged by size:
4810 2002 1516 1071 815 779 709 511
85 69
BsmI GAATG_Cn'
Cuts at: 3067 4069 4643 5422 5616 7849 8016 8742 9878
Size: 1002 574 779 194 2233 167 726 1136
Cuts at: 9878 9971 3067
Size: 93 5463
Fragments arranged by size:
5463 2233 1136 1002 779 726 574 194
167 93
BsmBI CGTCTCn'nnnn_
Cuts at: 6269 6269
Size: 12367
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3598 4027 4806 5317 5608 6026 6095
Size: 399 1808 429 779 511 291 418 69
Cuts at: 6095 6996 7611 8327 9613 9686 10428 10513 11584
Size: 901 615 716 1286 73 742 85 1071
Cuts at: 11584 1391
Size: 2174
Fragments arranged by size:
2174 1808 1286 1071 901 779 742 716
615 511 429 418 399 291 85 73
69
BspEI T'CCGG_A
Cuts at: 12030 12030
Size: 12367
BspHI T'CATG_A
Cuts at: 4609 10256 11986 4609
Size: 5647 1730 4990
Fragments arranged by size:
5647 4990 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2206 7484 8617 8965 9722 844
Size: 1362 5278 1133 348 757 3489
Fragments arranged by size:
5278 3489 1362 1133 757 348
BsrBI CCG'CTC
Cuts at: 1394 1823 10254 1394
Size: 429 8431 3507
Fragments arranged by size:
8431 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2545 2792 5833 6838 8728 9232 9355
Size: 1183 1297 247 3041 1005 1890 504 123
Cuts at: 9355 10862 11036 65
Size: 1507 174 1396
Fragments arranged by size:
3041 1890 1507 1396 1297 1183 1005 504
247 174 123
BsrFI r'CCGG_y
Cuts at: 1700 1810 6045 6689 11011 11198 1700
Size: 110 4235 644 4322 187 2869
Fragments arranged by size:
4322 4235 2869 644 187 110
BsrGI T'GTAC_A
Cuts at: 96 3349 96
Size: 3253 9114
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 12367
BssSI C'ACGA_G
Cuts at: 2019 10427 11439 2019
Size: 8408 1012 2947
Fragments arranged by size:
8408 2947 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 12367
BstBI TT'CG_AA
Cuts at: 4549 11171 4549
Size: 6622 5745
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 12367
BstXI CCAn_nnnn'nTGG
Cuts at: 2661 4117 7667 7766 9512 9545 2661
Size: 1456 3550 99 1746 33 5483
Fragments arranged by size:
5483 3550 1746 1456 99 33
BstYI r'GATC_y
Cuts at: 1972 2641 2671 3292 3648 4138 4375 4828 4958
Size: 669 30 621 356 490 237 453 130
Cuts at: 4958 7170 9016 9232 9515 9697 10064 10449 10466
Size: 2212 1846 216 283 182 367 385 17
Cuts at: 10466 11918 12004 12016 12362 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2212 1977 1846 1452 669 621 490 453
385 367 356 346 283 237 216 182
130 86 30 17 12
Bsu36I CC'TnA_GG
Cuts at: 3129 4029 3129
Size: 900 11467
BtgI C'CryG_G
Cuts at: 513 1065 2529 4648 4972 8194 513
Size: 552 1464 2119 324 3222 4686
Fragments arranged by size:
4686 3222 2119 1464 552 324
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2091 487
Size: 683 56 865 10763
Fragments arranged by size:
10763 865 683 56
BtsI GCAGTG_nn'
Cuts at: 2418 5039 5595 9264 9637 9883 10677 10697 2418
Size: 2621 556 3669 373 246 794 20 4088
Fragments arranged by size:
4088 3669 2621 794 556 373 246 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 12367
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 12332
DraI TTT'AAA
Cuts at: 2697 7107 9671 2697
Size: 4410 2564 5393
Fragments arranged by size:
5393 4410 2564
DraIII CAC_nnn'GTG
Cuts at: 3523 4494 9389 3523
Size: 971 4895 6501
Fragments arranged by size:
6501 4895 971
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8433 11374 809
Size: 507 7117 2941 1802
Fragments arranged by size:
7117 2941 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2220 2484 9601
Size: 54 1425 50 275 18 390 264 7117
Cuts at: 9601 9814 10703 12332 8
Size: 213 889 1629 43
Fragments arranged by size:
7117 1629 1425 889 390 275 264 213
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 9814 1537
Size: 275 8002 4090
Fragments arranged by size:
8002 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2811 3132 4945 6267 8734 8772 10297 1353
Size: 1458 321 1813 1322 2467 38 1525 3423
Fragments arranged by size:
3423 2467 1813 1525 1458 1322 321 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 10954 11328 11474 387
Size: 1064 138 9365 374 146 1280
Fragments arranged by size:
9365 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4804 4804
Size: 12367
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2622 2634 4163 4493 5046 5238 5594
Size: 393 990 12 1529 330 553 192 356
Cuts at: 5594 6213 6924 6929 8130 8339 9441 9504 10430
Size: 619 711 5 1201 209 1102 63 926
Cuts at: 10430 11027 11814 12082 1239
Size: 597 787 268 1524
Fragments arranged by size:
1529 1524 1201 1102 990 926 787 711
619 597 553 393 356 330 268 209
192 63 12 5
EcoO109I rG'GnC_Cy
Cuts at: 2326 8648 2326
Size: 6322 6045
EcoRI G'AATT_C
Cuts at: 6709 9066 9087 9676 10054 6709
Size: 2357 21 589 378 9022
Fragments arranged by size:
9022 2357 589 378 21
EcoRV GAT'ATC
Cuts at: 3090 3574 5679 6863 3090
Size: 484 2105 1184 8594
Fragments arranged by size:
8594 2105 1184 484
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3992 4177 3992
Size: 185 12182
FspI TGC'GCA
Cuts at: 10873 10873
Size: 12367
HaeII r_GCGC'y
Cuts at: 1678 3755 7354 7828 9130 11514 1678
Size: 2077 3599 474 1302 2384 2531
Fragments arranged by size:
3599 2531 2384 2077 1302 474
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2057 2089 2633 2665 3466
Size: 32 135 32 485 32 544 32 801
Cuts at: 3466 3498 4404 4436 4703 4735 4746 4778 5318
Size: 32 906 32 267 32 11 32 540
Cuts at: 5318 5350 6785 6817 11008 11040 11082 11114 1373
Size: 32 1435 32 4191 32 42 32 2626
Fragments arranged by size:
4191 2626 1435 906 801 544 540 485
267 135 42 32 32 32 32 32
32 32 32 32 32 32 32 11
HincII GTy'rAC
Cuts at: 669 1657 1938 4200 9711 9957 669
Size: 988 281 2262 5511 246 3079
Fragments arranged by size:
5511 3079 2262 988 281 246
HindIII A'AGCT_T
Cuts at: 748 5471 5520 7101 7110 8455 9411 748
Size: 4723 49 1581 9 1345 956 3704
Fragments arranged by size:
4723 3704 1581 1345 956 49 9
HpaI GTT'AAC
Cuts at: 4200 9957 4200
Size: 5757 6610
KasI G'GCGC_C
Cuts at: 7824 7824
Size: 12367
KpnI G_GTAC'C
Cuts at: 9692 9692
Size: 12367
MfeI C'AATT_G
Cuts at: 4737 9325 9966 4737
Size: 4588 641 7138
Fragments arranged by size:
7138 4588 641
MluI A'CGCG_T
Cuts at: 12243 12243
Size: 12367
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2014 2113 2208 3410 5927 6628 11481 11665
Size: 709 99 95 1202 2517 701 4853 184
Cuts at: 11665 1305
Size: 2007
Fragments arranged by size:
4853 2517 2007 1202 709 701 184 99
95
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2222 2486 9603 10
Size: 54 1425 343 390 264 7117 2774
Fragments arranged by size:
7117 2774 1425 390 343 264 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2578 3117 3555 3831 4728 4986 5635
Size: 648 1412 539 438 276 897 258 649
Cuts at: 5635 5752 9160 9543 10325 10684 10843 518
Size: 117 3408 383 782 359 159 2042
Fragments arranged by size:
3408 2042 1412 897 782 649 648 539
438 383 359 276 258 159 117
MspA1I CmG'CkG
Cuts at: 1606 3152 4012 4650 6458 8707 8715 9783 10460
Size: 1546 860 638 1808 2249 8 1068 677
Cuts at: 10460 11608 11853 1606
Size: 1148 245 2120
Fragments arranged by size:
2249 2120 1808 1546 1148 1068 860 677
638 245 8
NarI GG'CG_CC
Cuts at: 7825 7825
Size: 12367
NcoI C'CATG_G
Cuts at: 513 1065 2529 4972 513
Size: 552 1464 2443 7908
Fragments arranged by size:
7908 2443 1464 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 12367
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 12367
NotI GC'GGCC_GC
Cuts at: 9814 9814
Size: 12367
NsiI A_TGCA'T
Cuts at: 7309 8004 8224 7309
Size: 695 220 11452
Fragments arranged by size:
11452 695 220
NspI r_CATG'y
Cuts at: 1132 1229 2352 5644 8061 9725 11270 1132
Size: 97 1123 3292 2417 1664 1545 2229
Fragments arranged by size:
3292 2417 2229 1664 1545 1123 97
PasI CC'CwG_GG
Cuts at: 1348 6843 1348
Size: 5495 6872
PciI A'CATG_T
Cuts at: 8057 11266 8057
Size: 3209 9158
PflMI CCAn_nnn'nTGG
Cuts at: 1108 6647 8211 9299 1108
Size: 5539 1564 1088 4176
Fragments arranged by size:
5539 4176 1564 1088
PfoI T'CCnGG_A
Cuts at: 2968 4963 8422 2968
Size: 1995 3459 6913
Fragments arranged by size:
6913 3459 1995
PmeI GTTT'AAAC
Cuts at: 9671 9671
Size: 12367
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10436 10468 11949 11981 10436
Size: 32 1481 32 10822
Fragments arranged by size:
10822 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 8648 8648
Size: 12367
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 12367
PsiI TTA'TAA
Cuts at: 9937 9937
Size: 12367
PstI C_TGCA'G
Cuts at: 830 4837 8885 9222 9719 830
Size: 4007 4048 337 497 3478
Fragments arranged by size:
4048 4007 3478 497 337
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12367
PvuII CAG'CTG
Cuts at: 3152 4012 6458 8707 3152
Size: 860 2446 2249 6812
Fragments arranged by size:
6812 2446 2249 860
SacI G_AGCT'C
Cuts at: 4806 4806
Size: 12367
SacII CC_GC'GG
Cuts at: 4651 4651
Size: 12367
SalI G'TCGA_C
Cuts at: 1936 9709 1936
Size: 7773 4594
SapI GCTCTTCn'nnn_
Cuts at: 4945 4945
Size: 12367
SbfI CC_TGCA'GG
Cuts at: 9222 9719 9222
Size: 497 11870
ScaI AGT'ACT
Cuts at: 2825 5130 8484 10615 12327 2825
Size: 2305 3354 2131 1712 2865
Fragments arranged by size:
3354 2865 2305 2131 1712
SfcI C'TryA_G
Cuts at: 826 1045 3567 4371 4833 6428 8166 8881 9218
Size: 219 2522 804 462 1595 1738 715 337
Cuts at: 9218 9715 10850 11531 11722 826
Size: 497 1135 681 191 1471
Fragments arranged by size:
2522 1738 1595 1471 1135 804 715 681
497 462 337 219 191
SfoI GGC'GCC
Cuts at: 7826 7826
Size: 12367
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12367
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 12367
SmaI CCC'GGG
Cuts at: 1872 8089 9694 1872
Size: 6217 1605 4545
Fragments arranged by size:
6217 4545 1605
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2596 3467 7461 8452 8458 8494
Size: 197 926 653 871 3994 991 6 36
Cuts at: 8494 10471 11372 11634 820
Size: 1977 901 262 1553
Fragments arranged by size:
3994 1977 1553 991 926 901 871 653
262 197 36 6
SnaBI TAC'GTA
Cuts at: 493 10158 493
Size: 9665 2702
SpeI A'CTAG_T
Cuts at: 152 4264 152
Size: 4112 8255
SphI G_CATG'C
Cuts at: 1132 5644 9725 1132
Size: 4512 4081 3774
Fragments arranged by size:
4512 4081 3774
SspI AAT'ATT
Cuts at: 5 52 3003 6175 10291 5
Size: 47 2951 3172 4116 2081
Fragments arranged by size:
4116 3172 2951 2081 47
StuI AGG'CCT
Cuts at: 1517 2248 1517
Size: 731 11636
StyI C'CwwG_G
Cuts at: 513 1065 2529 4402 4972 6295 7177 9657 9806
Size: 552 1464 1873 570 1323 882 2480 149
Cuts at: 9806 513
Size: 3074
Fragments arranged by size:
3074 2480 1873 1464 1323 882 570 552
149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2245 6676 7592 9114 10394 10411 10564
Size: 1255 665 4431 916 1522 1280 17 153
Cuts at: 10564 10749 11194 325
Size: 185 445 1498
Fragments arranged by size:
4431 1522 1498 1280 1255 916 665 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2823 3349 5128
Size: 274 80 33 51 1443 846 526 1779
Cuts at: 5128 5380 6918 7776 8016 8482 9312 10613 12325
Size: 252 1538 858 240 466 830 1301 1712
Cuts at: 12325 96
Size: 138
Fragments arranged by size:
1779 1712 1538 1443 1301 858 846 830
526 466 274 252 240 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3125 3157 3633 3665 4945 4977 8694 8726 12327
Size: 32 476 32 1280 32 3717 32 3601
Cuts at: 12327 12359 3125
Size: 32 3133
Fragments arranged by size:
3717 3601 3133 1280 476 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 12367
XbaI T'CTAG_A
Cuts at: 9703 9703
Size: 12367
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2063 2564 5005 5201 6034 6670 7667 7766 8569
Size: 501 2441 196 833 636 997 99 803
Cuts at: 8569 2063
Size: 5861
Fragments arranged by size:
5861 2441 997 833 803 636 501 196
99
XhoI C'TCGA_G
Cuts at: 1943 7461 1943
Size: 5518 6849
XmaI C'CCGG_G
Cuts at: 1870 8087 9692 1870
Size: 6217 1605 4545
Fragments arranged by size:
6217 4545 1605
XmnI GAAnn'nnTTC
Cuts at: 2847 3678 4545 6198 7013 8313 10496 2847
Size: 831 867 1653 815 1300 2183 4718
Fragments arranged by size:
4718 2183 1653 1300 867 831 815
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 10175 276
Size: 53 83 186 936 8641 2468
Fragments arranged by size:
8641 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbvCI Bpu10I BsiWI BstZ17I
EcoNI FseI FspAI NaeI NgoMIV NruI PacI PmlI
PspOMI PspXI PsrI RsrII SanDI SexAI SfiI SrfI
SwaI