Restriction map for
pFN21AE3822
(Circular) (Six-base) MAPSORT of: pFN21AE3822.seq Check: 8514 from: 1 to: 12286
pFN21AE3822 12286 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2230 2230
Size: 12286
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 10096 278
Size: 53 83 186 936 8560 2468
Fragments arranged by size:
8560 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 9081 9331 9629 1937
Size: 7144 250 298 4594
Fragments arranged by size:
7144 4594 298 250
Acc65I G'GTAC_C
Cuts at: 9607 9607
Size: 12286
AclI AA'CG_TT
Cuts at: 1563 10413 10786 1563
Size: 8850 373 3063
Fragments arranged by size:
8850 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2072 2421 2432 3393 4620 4851 4862 5126
Size: 440 349 11 961 1227 231 11 264
Cuts at: 5126 5408 5417 6303 7257 7281 7865 8148 8583
Size: 282 9 886 954 24 584 283 435
Cuts at: 8583 9245 9465 10349 11733 12001 1632
Size: 662 220 884 1384 268 1917
Fragments arranged by size:
1917 1384 1227 961 954 886 884 662
584 440 435 349 283 282 268 264
231 220 24 11 11 9
AfeI AGC'GCT
Cuts at: 2940 2940
Size: 12286
AflII C'TTAA_G
Cuts at: 820 1017 4375 5668 820
Size: 197 3358 1293 7438
Fragments arranged by size:
7438 3358 1293 197
AflIII A'CryG_T
Cuts at: 1203 2557 6939 9340 11185 12162 1203
Size: 1354 4382 2401 1845 977 1327
Fragments arranged by size:
4382 2401 1845 1354 1327 977
AgeI A'CCGG_T
Cuts at: 11117 11117
Size: 12286
AhdI GACnn_n'nnGTC
Cuts at: 11015 11015
Size: 12286
AleI CACnn'nnGTG
Cuts at: 1166 2257 5315 1166
Size: 1091 3058 8137
Fragments arranged by size:
8137 3058 1091
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 7941 1184
Size: 6757 5529
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5543 5575 5543
Size: 32 12254
AlwNI CAG_nnn'CTG
Cuts at: 3817 10029 11601 3817
Size: 6212 1572 4502
Fragments arranged by size:
6212 4502 1572
ApaI G_GGCC'C
Cuts at: 4818 4818
Size: 12286
ApaLI G'TGCA_C
Cuts at: 8121 9173 10343 11499 8121
Size: 1052 1170 1156 8908
Fragments arranged by size:
8908 1170 1156 1052
ApoI r'AATT_y
Cuts at: 1493 2597 2644 2799 3578 3807 3891 4591 4616
Size: 1104 47 155 779 229 84 700 25
Cuts at: 4616 4748 4786 5141 5491 5555 6203 6635 6902
Size: 132 38 355 350 64 648 432 267
Cuts at: 6902 6923 6968 7631 8401 8785 8926 9520 9595
Size: 21 45 663 770 384 141 594 75
Cuts at: 9595 9821 9973 12082 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1104 779 770 700 663 648
594 432 384 355 350 267 229 226
155 152 141 132 84 75 64 47
45 38 25 21
AseI AT'TA_AT
Cuts at: 160 1032 2267 6065 7127 8333 10840 160
Size: 872 1235 3798 1062 1206 2507 1606
Fragments arranged by size:
3798 2507 1606 1235 1206 1062 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12286
AvaI C'yCGr_G
Cuts at: 1870 1943 9611 1870
Size: 73 7668 4545
Fragments arranged by size:
7668 4545 73
AvrII C'CTAG_G
Cuts at: 7657 7657
Size: 12286
BamHI G'GATC_C
Cuts at: 9616 9983 9616
Size: 367 11919
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 9607 11062 618
Size: 325 217 627 7820 1455 1842
Fragments arranged by size:
7820 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4818 9605 1391
Size: 3427 4787 4072
Fragments arranged by size:
4787 4072 3427
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2420 4035 928
Size: 971 521 1615 9179
Fragments arranged by size:
9179 1615 971 521
BbvCI CC'TCA_GC
Cuts at: 6266 6266
Size: 12286
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 10477 10511 1903
Size: 34 8540 34 3678
Fragments arranged by size:
8540 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3565 3801 3830 8031 8561 10178 11388 3565
Size: 236 29 4201 530 1617 1210 4463
Fragments arranged by size:
4463 4201 1617 1210 530 236 29
BclI T'GATC_A
Cuts at: 1548 4156 4533 4825 1548
Size: 2608 377 292 9009
Fragments arranged by size:
9009 2608 377 292
BfrBI ATG'CAT
Cuts at: 3226 3266 3731 8466 3226
Size: 40 465 4735 7046
Fragments arranged by size:
7046 4735 465 40
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 10897 136
Size: 107 122 71 631 605 9225 1525
Fragments arranged by size:
9225 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2422 2931 6085 6163 7516 12281 2422
Size: 509 3154 78 1353 4765 2427
Fragments arranged by size:
4765 3154 2427 1353 509 78
BlpI GC'TnA_GC
Cuts at: 2942 8608 9102 9703 2942
Size: 5666 494 601 5525
Fragments arranged by size:
5666 5525 601 494
Bme1580I G_kGCm'C
Cuts at: 1790 3124 4818 8108 8125 8311 8523 9177 10347
Size: 1334 1694 3290 17 186 212 654 1170
Cuts at: 10347 11503 1790
Size: 1156 2573
Fragments arranged by size:
3290 2573 1694 1334 1170 1156 654 212
186 17
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 12286
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6042 7788 10017
Size: 416 53 83 399 24 4625 1746 2229
Cuts at: 10017 10975 442
Size: 958 1753
Fragments arranged by size:
4625 2229 1753 1746 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 12286
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2173 2593 7870 8555 2173
Size: 420 5277 685 5904
Fragments arranged by size:
5904 5277 685 420
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2141 3530 5072 5351 7412 7629 8232 9312
Size: 902 1389 1542 279 2061 217 603 1080
Cuts at: 9312 10946 1239
Size: 1634 2579
Fragments arranged by size:
2579 2061 1634 1542 1389 1080 902 603
279 217
Bpu10I CC'TnA_GC
Cuts at: 5003 6266 5003
Size: 1263 11023
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5401 5471 5734 7163 8346 10411 11276 11574 5401
Size: 70 263 1429 1183 2065 865 298 6113
Fragments arranged by size:
6113 2065 1429 1183 865 298 263 70
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7236 7977 10949 882
Size: 617 5737 741 2972 2219
Fragments arranged by size:
5737 2972 2219 741 617
BsaAI yAC'GTr
Cuts at: 493 1204 2142 3100 7936 9341 10077 493
Size: 711 938 958 4836 1405 736 2702
Fragments arranged by size:
4836 2702 1405 958 938 736 711
BsaBI GATnn'nnATC
Cuts at: 4209 5702 12280 4209
Size: 1493 6578 4215
Fragments arranged by size:
6578 4215 1493
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 10093 10475 11110 275
Size: 53 83 186 936 8560 382 635 1451
Fragments arranged by size:
8560 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6651 9432 10719 11117 11391 11538 11949 6651
Size: 2781 1287 398 274 147 411 6988
Fragments arranged by size:
6988 2781 1287 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 7695 7725 1178
Size: 30 165 30 6292 30 5739
Fragments arranged by size:
6292 5739 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2417 2597 2948 9309 1186
Size: 1231 180 351 6361 4163
Fragments arranged by size:
6361 4163 1231 351 180
BseYI C'CCAG_C
Cuts at: 3117 3190 6766 7817 8509 11489 11985 3117
Size: 73 3576 1051 692 2980 496 3418
Fragments arranged by size:
3576 3418 2980 1051 692 496 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2255 2921 3122 3524 1163
Size: 573 519 666 201 402 9925
Fragments arranged by size:
9925 666 573 519 402 201
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 9736 10497 11525 1540
Size: 275 186 7735 761 1028 2301
Fragments arranged by size:
7735 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2154 7183 8125 9177 10347 10432 11503 2154
Size: 5029 942 1052 1170 85 1071 2937
Fragments arranged by size:
5029 2937 1170 1071 1052 942 85
BsmI GAATG_Cn'
Cuts at: 2780 2923 3226 3268 4187 4912 5071 6099 6475
Size: 143 303 42 919 725 159 1028 376
Cuts at: 6475 8468 8500 9797 9890 2780
Size: 1993 32 1297 93 5176
Fragments arranged by size:
5176 1993 1297 1028 919 725 376 303
159 143 93 42 32
BsmBI CGTCTCn'nnnn_
Cuts at: 8422 9562 8422
Size: 1140 11146
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2154 3124 4818 7183 8108 8125 8311
Size: 399 364 970 1694 2365 925 17 186
Cuts at: 8311 8523 9177 9605 10347 10432 11503 1391
Size: 212 654 428 742 85 1071 2174
Fragments arranged by size:
2365 2174 1694 1071 970 925 742 654
428 399 364 212 186 85 17
BspEI T'CCGG_A
Cuts at: 11949 11949
Size: 12286
BspHI T'CATG_A
Cuts at: 2730 3484 4828 9375 10175 11905 2730
Size: 754 1344 4547 800 1730 3111
Fragments arranged by size:
4547 3111 1730 1344 800 754
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2230 6182 7602 9641 844
Size: 1386 3952 1420 2039 3489
Fragments arranged by size:
3952 3489 2039 1420 1386
BsrBI CCG'CTC
Cuts at: 1394 1823 10173 1394
Size: 429 8350 3507
Fragments arranged by size:
8350 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2746 2924 5626 8037 8061 10781 10955
Size: 1183 1498 178 2702 2411 24 2720 174
Cuts at: 10955 65
Size: 1396
Fragments arranged by size:
2720 2702 2411 1498 1396 1183 178 174
24
BsrFI r'CCGG_y
Cuts at: 1700 1810 10930 11117 1700
Size: 110 9120 187 2869
Fragments arranged by size:
9120 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3715 96
Size: 3619 8667
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 12286
BssSI C'ACGA_G
Cuts at: 4302 5074 10346 11358 4302
Size: 772 5272 1012 5230
Fragments arranged by size:
5272 5230 1012 772
BstAPI GCAn_nnn'nTGC
Cuts at: 794 6396 8048 794
Size: 5602 1652 5032
Fragments arranged by size:
5602 5032 1652
BstBI TT'CG_AA
Cuts at: 11090 11090
Size: 12286
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 12286
BstXI CCAn_nnnn'nTGG
Cuts at: 2318 2540 4054 4276 4810 5866 2318
Size: 222 1514 222 534 1056 8738
Fragments arranged by size:
8738 1514 1056 534 222 222
BstYI r'GATC_y
Cuts at: 1972 2125 2422 2931 4226 4298 5233 5849 5890
Size: 153 297 509 1295 72 935 616 41
Cuts at: 5890 6085 6163 6540 7256 7516 9316 9616 9983
Size: 195 78 377 716 260 1800 300 367
Cuts at: 9983 10368 10385 11837 11923 11935 12281 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1800 1452 1295 935 716 616 509
385 377 367 346 300 297 260 195
153 86 78 72 41 17 12
Bsu36I CC'TnA_GG
Cuts at: 4576 6870 4576
Size: 2294 9992
BtgI C'CryG_G
Cuts at: 513 1065 2004 8312 513
Size: 552 939 6308 4487
Fragments arranged by size:
6308 4487 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4599 487
Size: 683 56 3373 8174
Fragments arranged by size:
8174 3373 683 56
BtsI GCAGTG_nn'
Cuts at: 3456 3779 6770 9084 9270 9802 10596 10616 3456
Size: 323 2991 2314 186 532 794 20 5126
Fragments arranged by size:
5126 2991 2314 794 532 323 186 20
ClaI AT'CG_AT
Cuts at: 1554 7135 1554
Size: 5581 6705
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 12251
DraI TTT'AAA
Cuts at: 2650 5406 5583 5929 9590 2650
Size: 2756 177 346 3661 5346
Fragments arranged by size:
5346 3661 2756 346 177
DraIII CAC_nnn'GTG
Cuts at: 6466 8002 6466
Size: 1536 10750
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 11293 809
Size: 507 9977 1802
Fragments arranged by size:
9977 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3181 4398 9733
Size: 54 1425 50 275 18 1351 1217 5335
Cuts at: 9733 10622 12251 8
Size: 889 1629 43
Fragments arranged by size:
5335 1629 1425 1351 1217 889 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 9733 1537
Size: 275 7921 4090
Fragments arranged by size:
7921 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2028 2652 4651 5102 6314 10216 1353
Size: 675 624 1999 451 1212 3902 3423
Fragments arranged by size:
3902 3423 1999 1212 675 624 451
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 10873 11247 11393 387
Size: 1064 138 9284 374 146 1280
Fragments arranged by size:
9284 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2141 2421 2432 3393 3530 4620
Size: 393 440 69 280 11 961 137 1090
Cuts at: 4620 4851 4862 5072 5126 5351 5408 5417 6303
Size: 231 11 210 54 225 57 9 886
Cuts at: 6303 7257 7281 7412 7629 7865 8148 8232 8583
Size: 954 24 131 217 236 283 84 351
Cuts at: 8583 9245 9312 9465 10349 10946 11733 12001 1239
Size: 662 67 153 884 597 787 268 1524
Fragments arranged by size:
1524 1090 961 954 886 884 787 662
597 440 393 351 283 280 268 236
231 225 217 210 153 137 131 84
69 67 57 54 24 11 11 9
EcoNI CCTnn'n_nnAGG
Cuts at: 6269 6269
Size: 12286
EcoO109I rG'GnC_Cy
Cuts at: 2306 4814 5642 2306
Size: 2508 828 8950
Fragments arranged by size:
8950 2508 828
EcoRI G'AATT_C
Cuts at: 2799 6203 8401 9595 9973 2799
Size: 3404 2198 1194 378 5112
Fragments arranged by size:
5112 3404 2198 1194 378
EcoRV GAT'ATC
Cuts at: 4541 7803 4541
Size: 3262 9024
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3444 7882 8391 3444
Size: 4438 509 7339
Fragments arranged by size:
7339 4438 509
FspI TGC'GCA
Cuts at: 10792 10792
Size: 12286
HaeII r_GCGC'y
Cuts at: 1678 2942 3518 11433 1678
Size: 1264 576 7915 2531
Fragments arranged by size:
7915 2531 1264 576
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2407 2439 3728 3760 4525
Size: 32 135 32 835 32 1289 32 765
Cuts at: 4525 4557 4595 4627 5785 5817 6153 6185 6532
Size: 32 38 32 1158 32 336 32 347
Cuts at: 6532 6564 6667 6699 7248 7280 8130 8162 9458
Size: 32 103 32 549 32 850 32 1296
Cuts at: 9458 9490 10927 10959 11001 11033 1373
Size: 32 1437 32 42 32 2626
Fragments arranged by size:
2626 1437 1296 1289 1158 850 835 765
549 347 336 135 103 42 38 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2640 3273 4329 9332 9630 9876
Size: 988 281 702 633 1056 5003 298 246
Cuts at: 9876 669
Size: 3079
Fragments arranged by size:
5003 3079 1056 988 702 633 298 281
246
HindIII A'AGCT_T
Cuts at: 748 2296 2550 7239 8846 748
Size: 1548 254 4689 1607 4188
Fragments arranged by size:
4689 4188 1607 1548 254
HpaI GTT'AAC
Cuts at: 2640 9876 2640
Size: 7236 5050
KpnI G_GTAC'C
Cuts at: 9611 9611
Size: 12286
MfeI C'AATT_G
Cuts at: 2351 6630 9885 2351
Size: 4279 3255 4752
Fragments arranged by size:
4752 4279 3255
MluI A'CGCG_T
Cuts at: 12162 12162
Size: 12286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2299 5276 6541 7105 7608 8779 9084 11400
Size: 994 2977 1265 564 503 1171 305 2316
Cuts at: 11400 11584 1305
Size: 184 2007
Fragments arranged by size:
2977 2316 2007 1265 1171 994 564 503
305 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3183 4400 10
Size: 54 1425 343 1351 1217 7896
Fragments arranged by size:
7896 1425 1351 1217 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2257 3076 3221 3333 5064 5315 5731
Size: 648 1091 819 145 112 1731 251 416
Cuts at: 5731 6119 6366 8905 9071 9539 10244 10603 10762
Size: 388 247 2539 166 468 705 359 159
Cuts at: 10762 518
Size: 2042
Fragments arranged by size:
2539 2042 1731 1091 819 705 648 468
416 388 359 251 247 166 159 145
112
MspA1I CmG'CkG
Cuts at: 1606 2019 2946 3508 5287 6568 6688 7817 8408
Size: 413 927 562 1779 1281 120 1129 591
Cuts at: 8408 8807 9702 10379 11527 11772 1606
Size: 399 895 677 1148 245 2120
Fragments arranged by size:
2120 1779 1281 1148 1129 927 895 677
591 562 413 399 245 120
NcoI C'CATG_G
Cuts at: 513 1065 2004 8312 513
Size: 552 939 6308 4487
Fragments arranged by size:
6308 4487 939 552
NdeI CA'TA_TG
Cuts at: 387 4905 387
Size: 4518 7768
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 12286
NotI GC'GGCC_GC
Cuts at: 9733 9733
Size: 12286
NruI TCG'CGA
Cuts at: 2228 2228
Size: 12286
NsiI A_TGCA'T
Cuts at: 3228 3268 3733 8468 3228
Size: 40 465 4735 7046
Fragments arranged by size:
7046 4735 465 40
NspI r_CATG'y
Cuts at: 1132 1229 2561 3117 3553 6943 7162 9644 11189
Size: 97 1332 556 436 3390 219 2482 1545
Cuts at: 11189 1132
Size: 2229
Fragments arranged by size:
3390 2482 2229 1545 1332 556 436 219
97
PacI TTA_AT'TAA
Cuts at: 8337 8337
Size: 12286
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 12286
PciI A'CATG_T
Cuts at: 2557 6939 11185 2557
Size: 4382 4246 3658
Fragments arranged by size:
4382 4246 3658
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2119 7345 1108
Size: 1011 5226 6049
Fragments arranged by size:
6049 5226 1011
PfoI T'CCnGG_A
Cuts at: 2082 2118 9312 2082
Size: 36 7194 5056
Fragments arranged by size:
7194 5056 36
PmeI GTTT'AAAC
Cuts at: 9590 9590
Size: 12286
PmlI CAC'GTG
Cuts at: 3100 3100
Size: 12286
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10355 10387 11868 11900 10355
Size: 32 1481 32 10741
Fragments arranged by size:
10741 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2306 5642 2306
Size: 3336 8950
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 12286
PsiI TTA'TAA
Cuts at: 2754 6000 8154 9856 2754
Size: 3246 2154 1702 5184
Fragments arranged by size:
5184 3246 2154 1702
PspOMI G'GGCC_C
Cuts at: 4814 4814
Size: 12286
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4299 4331 4299
Size: 32 12254
PstI C_TGCA'G
Cuts at: 830 3786 6693 6777 9638 830
Size: 2956 2907 84 2861 3478
Fragments arranged by size:
3478 2956 2907 2861 84
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12286
PvuII CAG'CTG
Cuts at: 2019 2946 3508 5287 6568 6688 7817 8408 2019
Size: 927 562 1779 1281 120 1129 591 5897
Fragments arranged by size:
5897 1779 1281 1129 927 591 562 120
SalI G'TCGA_C
Cuts at: 1936 9330 9628 1936
Size: 7394 298 4594
Fragments arranged by size:
7394 4594 298
SanDI GG'GwC_CC
Cuts at: 2306 2306
Size: 12286
SapI GCTCTTCn'nnn_
Cuts at: 6314 6314
Size: 12286
SbfI CC_TGCA'GG
Cuts at: 9638 9638
Size: 12286
ScaI AGT'ACT
Cuts at: 6214 8072 8896 10534 12246 6214
Size: 1858 824 1638 1712 6254
Fragments arranged by size:
6254 1858 1712 1638 824
SexAI A'CCwGG_T
Cuts at: 5638 5638
Size: 12286
SfcI C'TryA_G
Cuts at: 826 1045 3782 5238 6208 6689 6773 7913 8077
Size: 219 2737 1456 970 481 84 1140 164
Cuts at: 8077 9634 10769 11450 11641 826
Size: 1557 1135 681 191 1471
Fragments arranged by size:
2737 1557 1471 1456 1140 1135 970 681
481 219 191 164 84
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12286
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 12286
SmaI CCC'GGG
Cuts at: 1872 9613 1872
Size: 7741 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4375 5380 5450 5668 5713 7142
Size: 197 926 2432 1005 70 218 45 1429
Cuts at: 7142 8361 10390 11291 11553 820
Size: 1219 2029 901 262 1553
Fragments arranged by size:
2432 2029 1553 1429 1219 1005 926 901
262 218 197 70 45
SnaBI TAC'GTA
Cuts at: 493 10077 493
Size: 9584 2702
SpeI A'CTAG_T
Cuts at: 152 6670 152
Size: 6518 5768
SphI G_CATG'C
Cuts at: 1132 3117 3553 9644 1132
Size: 1985 436 6091 3774
Fragments arranged by size:
6091 3774 1985 436
SspI AAT'ATT
Cuts at: 5 52 2758 4029 4860 7001 7606 8601 10210
Size: 47 2706 1271 831 2141 605 995 1609
Cuts at: 10210 5
Size: 2081
Fragments arranged by size:
2706 2141 2081 1609 1271 995 831 605
47
StuI AGG'CCT
Cuts at: 1517 7036 8371 1517
Size: 5519 1335 5432
Fragments arranged by size:
5519 5432 1335
StyI C'CwwG_G
Cuts at: 513 1065 2004 2338 4417 4809 6302 6414 7541
Size: 552 939 334 2079 392 1493 112 1127
Cuts at: 7541 7657 7857 7866 8312 8613 9725 513
Size: 116 200 9 446 301 1112 3074
Fragments arranged by size:
3074 2079 1493 1127 1112 939 552 446
392 334 301 200 116 112 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 9355 10313 10330 10483 10668 11113 325
Size: 1255 7775 958 17 153 185 445 1498
Fragments arranged by size:
7775 1498 1255 958 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3715 6212 6789
Size: 274 80 33 51 1443 1738 2497 577
Cuts at: 6789 8070 8894 10532 12244 96
Size: 1281 824 1638 1712 138
Fragments arranged by size:
2497 1738 1712 1638 1443 1281 824 577
274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5065 5097 5208 5240 5414 5446 6791 6823 7695
Size: 32 111 32 174 32 1345 32 872
Cuts at: 7695 7727 8005 8037 12246 12278 5065
Size: 32 278 32 4209 32 5073
Fragments arranged by size:
5073 4209 1345 872 278 174 111 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 8662 1316
Size: 7346 4940
XbaI T'CTAG_A
Cuts at: 5738 9622 5738
Size: 3884 8402
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 7113 7864 7940 7113
Size: 751 76 11459
Fragments arranged by size:
11459 751 76
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 12286
XmaI C'CCGG_G
Cuts at: 1870 9611 1870
Size: 7741 4545
XmnI GAAnn'nnTTC
Cuts at: 2780 2799 3659 3860 4594 6880 6903 7377 10415
Size: 19 860 201 734 2286 23 474 3038
Cuts at: 10415 2780
Size: 4651
Fragments arranged by size:
4651 3038 2286 860 734 474 201 23
19
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 10094 276
Size: 53 83 186 936 8560 2468
Fragments arranged by size:
8560 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SalI SanDI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BbeI BsiWI BstZ17I EcoICRI FseI FspAI
KasI NaeI NarI NgoMIV PspXI RsrII SacI SacII
SfiI SfoI SrfI SwaI