Restriction map for
pFN21AE3818
(Circular) (Six-base) MAPSORT of: pFN21AE3818.seq Check: 4159 from: 1 to: 8251
pFN21AE3818 8251 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2005 2005
Size: 8251
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3408 6061 278
Size: 53 83 186 936 1872 2653 2468
Fragments arranged by size:
2653 2468 1872 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5334 5594 1937
Size: 3397 260 4594
Fragments arranged by size:
4594 3397 260
Acc65I G'GTAC_C
Cuts at: 2523 5572 2523
Size: 3049 5202
AclI AA'CG_TT
Cuts at: 1563 6378 6751 1563
Size: 4815 373 3063
Fragments arranged by size:
4815 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2701 3672 3770 3946 5448 6314 7698 7966
Size: 1069 971 98 176 1502 866 1384 268
Cuts at: 7966 1632
Size: 1917
Fragments arranged by size:
1917 1502 1384 1069 971 866 268 176
98
AflII C'TTAA_G
Cuts at: 820 1017 3292 820
Size: 197 2275 5779
Fragments arranged by size:
5779 2275 197
AflIII A'CryG_T
Cuts at: 1203 2650 7150 8127 1203
Size: 1447 4500 977 1327
Fragments arranged by size:
4500 1447 1327 977
AgeI A'CCGG_T
Cuts at: 7082 7082
Size: 8251
AhdI GACnn_n'nnGTC
Cuts at: 5290 6980 5290
Size: 1690 6561
AleI CACnn'nnGTG
Cuts at: 1166 2495 2797 4150 1166
Size: 1329 302 1353 5267
Fragments arranged by size:
5267 1353 1329 302
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8251
AlwNI CAG_nnn'CTG
Cuts at: 2759 2948 4416 5471 5474 5994 7566 2759
Size: 189 1468 1055 3 520 1572 3444
Fragments arranged by size:
3444 1572 1468 1055 520 189 3
ApaLI G'TGCA_C
Cuts at: 3843 5298 6308 7464 3843
Size: 1455 1010 1156 4630
Fragments arranged by size:
4630 1455 1156 1010
ApoI r'AATT_y
Cuts at: 1493 2987 3106 3214 3341 3805 4874 5402 5560
Size: 1494 119 108 127 464 1069 528 158
Cuts at: 5560 5786 5938 8047 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1494 1069 528 464 226 158
152 127 119 108
AseI AT'TA_AT
Cuts at: 160 1032 6805 160
Size: 872 5773 1606
Fragments arranged by size:
5773 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8251
AvaI C'yCGr_G
Cuts at: 1870 1943 5483 5576 1870
Size: 73 3540 93 4545
Fragments arranged by size:
4545 3540 93 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2639 2672 5187 5220 2639
Size: 33 2515 33 5670
Fragments arranged by size:
5670 2515 33 33
BamHI G'GATC_C
Cuts at: 2067 3860 5581 5948 2067
Size: 1793 1721 367 4370
Fragments arranged by size:
4370 1793 1721 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2473 2523 4141 5572 7027
Size: 325 217 627 686 50 1618 1431 1455
Cuts at: 7027 618
Size: 1842
Fragments arranged by size:
1842 1618 1455 1431 686 627 325 217
50
BanII G_rGCy'C
Cuts at: 1391 2046 2294 3581 5570 1391
Size: 655 248 1287 1989 4072
Fragments arranged by size:
4072 1989 1287 655 248
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 7280
BbvCI CC'TCA_GC
Cuts at: 2055 3663 2055
Size: 1608 6643
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4123 4157 6442 6476 1903
Size: 34 2186 34 2285 34 3678
Fragments arranged by size:
3678 2285 2186 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6143 7353 6143
Size: 1210 7041
BclI T'GATC_A
Cuts at: 1548 2161 1548
Size: 613 7638
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4741 5177 6862
Size: 107 122 71 631 605 3069 436 1685
Cuts at: 6862 136
Size: 1525
Fragments arranged by size:
3069 1685 1525 631 605 436 122 107
71
BglII A'GATC_T
Cuts at: 3229 8246 3229
Size: 5017 3234
BlpI GC'TnA_GC
Cuts at: 2564 5668 2564
Size: 3104 5147
Bme1580I G_kGCm'C
Cuts at: 1790 3790 3847 4696 4954 5097 5302 5319 5484
Size: 2000 57 849 258 143 205 17 165
Cuts at: 5484 6312 7468 1790
Size: 828 1156 2573
Fragments arranged by size:
2573 2000 1156 849 828 258 205 165
143 57 17
BmgBI CAC'GTC
Cuts at: 1318 2651 4840 1318
Size: 1333 2189 4729
Fragments arranged by size:
4729 2189 1333
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2489 4295 5982
Size: 416 53 83 399 24 1072 1806 1687
Cuts at: 5982 6940 442
Size: 958 1753
Fragments arranged by size:
1806 1753 1687 1072 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8251
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4005 5257 4005
Size: 1252 6999
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2030 2229 3330 3354 3625 4418 4617 4671
Size: 791 199 1101 24 271 793 199 54
Cuts at: 4671 4731 4734 4815 4932 6911 1239
Size: 60 3 81 117 1979 2579
Fragments arranged by size:
2579 1979 1101 793 791 271 199 199
117 81 60 54 24 3
Bpu10I CC'TnA_GC
Cuts at: 2055 2636 3663 5365 2055
Size: 581 1027 1702 4941
Fragments arranged by size:
4941 1702 1027 581
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5121 6376 7241 7539 5121
Size: 1255 865 298 5833
Fragments arranged by size:
5833 1255 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2126 4537 6914 882
Size: 617 627 2411 2377 2219
Fragments arranged by size:
2411 2377 2219 627 617
BsaAI yAC'GTr
Cuts at: 493 1204 6042 493
Size: 711 4838 2702
Fragments arranged by size:
4838 2702 711
BsaBI GATnn'nnATC
Cuts at: 8245 8245
Size: 8251
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2919 3405 6058 6440
Size: 53 83 186 936 1386 486 2653 382
Cuts at: 6440 7075 275
Size: 635 1451
Fragments arranged by size:
2653 1451 1386 936 635 486 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 6684 7082 7356 7503 7914 6684
Size: 398 274 147 411 7021
Fragments arranged by size:
7021 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2732 2762 1178
Size: 30 165 30 1329 30 6667
Fragments arranged by size:
6667 1329 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3676 3680 4584 1186
Size: 2490 4 904 4853
Fragments arranged by size:
4853 2490 904 4
BseYI C'CCAG_C
Cuts at: 2154 2469 2788 3865 3899 4122 4226 5111 7454
Size: 315 319 1077 34 223 104 885 2343
Cuts at: 7454 7950 2154
Size: 496 2455
Fragments arranged by size:
2455 2343 1077 885 496 319 315 223
104 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2103 2568 4862 5564 1163
Size: 573 367 465 2294 702 3850
Fragments arranged by size:
3850 2294 702 573 465 367
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2101 5701 6462 7490 1540
Size: 275 186 100 3600 761 1028 2301
Fragments arranged by size:
3600 2301 1028 761 275 186 100
BsiHKAI G_wGCw'C
Cuts at: 2294 3847 5302 6312 6397 7468 2294
Size: 1553 1455 1010 85 1071 3077
Fragments arranged by size:
3077 1553 1455 1071 1010 85
BsmI GAATG_Cn'
Cuts at: 3134 5762 5855 3134
Size: 2628 93 5530
Fragments arranged by size:
5530 2628 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2294 3581 3790 3847 4696 4954
Size: 399 256 248 1287 209 57 849 258
Cuts at: 4954 5097 5302 5319 5484 5570 6312 6397 7468
Size: 143 205 17 165 86 742 85 1071
Cuts at: 7468 1391
Size: 2174
Fragments arranged by size:
2174 1287 1071 849 742 399 258 256
248 209 205 165 143 86 85 57
17
BspEI T'CCGG_A
Cuts at: 7914 7914
Size: 8251
BspHI T'CATG_A
Cuts at: 3972 6140 7870 3972
Size: 2168 1730 4353
Fragments arranged by size:
4353 2168 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2005 3717 4289 5183 5606 844
Size: 1161 1712 572 894 423 3489
Fragments arranged by size:
3489 1712 1161 894 572 423
BsrBI CCG'CTC
Cuts at: 1394 1823 6138 1394
Size: 429 4315 3507
Fragments arranged by size:
4315 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3198 4404 6746 6920 65
Size: 1183 1950 1206 2342 174 1396
Fragments arranged by size:
2342 1950 1396 1206 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2135 6895 7082 1700
Size: 110 325 4760 187 2869
Fragments arranged by size:
4760 2869 325 187 110
BsrGI T'GTAC_A
Cuts at: 96 2737 5521 96
Size: 2641 2784 2826
Fragments arranged by size:
2826 2784 2641
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8251
BssSI C'ACGA_G
Cuts at: 6311 7323 6311
Size: 1012 7239
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3794 794
Size: 3000 5251
BstBI TT'CG_AA
Cuts at: 7055 7055
Size: 8251
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8251
BstXI CCAn_nnnn'nTGG
Cuts at: 2179 2179
Size: 8251
BstYI r'GATC_y
Cuts at: 1972 2067 3229 3860 4606 5581 5948 6333 6350
Size: 95 1162 631 746 975 367 385 17
Cuts at: 6350 7802 7888 7900 8246 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1162 975 746 631 385 367
346 95 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3525 3540 4369 3525
Size: 15 829 7407
Fragments arranged by size:
7407 829 15
BtgI C'CryG_G
Cuts at: 513 1065 2004 2062 2527 2973 513
Size: 552 939 58 465 446 5791
Fragments arranged by size:
5791 939 552 465 446 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7512
Fragments arranged by size:
7512 683 56
BtsI GCAGTG_nn'
Cuts at: 2086 2551 2676 3869 4221 4222 5097 5767 6561
Size: 465 125 1193 352 1 875 670 794
Cuts at: 6561 6581 2086
Size: 20 3756
Fragments arranged by size:
3756 1193 875 794 670 465 352 125
20 1
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8251
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8216
DraI TTT'AAA
Cuts at: 5555 5555
Size: 8251
DraIII CAC_nnn'GTG
Cuts at: 2546 4795 2546
Size: 2249 6002
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7258 809
Size: 507 5942 1802
Fragments arranged by size:
5942 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2098 3171 5178
Size: 54 1425 50 275 18 268 1073 2007
Cuts at: 5178 5698 6587 8216 8
Size: 520 889 1629 43
Fragments arranged by size:
2007 1629 1425 1073 889 520 275 268
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2098 5698 1537
Size: 275 286 3600 4090
Fragments arranged by size:
4090 3600 286 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2342 3135 3648 3974 4465 4467 4587 5010
Size: 989 793 513 326 491 2 120 423
Cuts at: 5010 6181 1353
Size: 1171 3423
Fragments arranged by size:
3423 1171 989 793 513 491 423 326
120 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6838 7212 7358 387
Size: 1064 138 5249 374 146 1280
Fragments arranged by size:
5249 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2292 2292
Size: 8251
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2030 2229 2701 3330 3354 3625 3672
Size: 393 398 199 472 629 24 271 47
Cuts at: 3672 3770 3946 4418 4617 4671 4731 4734 4815
Size: 98 176 472 199 54 60 3 81
Cuts at: 4815 4932 5448 6314 6911 7698 7966 1239
Size: 117 516 866 597 787 268 1524
Fragments arranged by size:
1524 866 787 629 597 516 472 472
398 393 271 268 199 199 176 117
98 81 60 54 47 24 3
EcoNI CCTnn'n_nnAGG
Cuts at: 3479 3479
Size: 8251
EcoO109I rG'GnC_Cy
Cuts at: 2091 2110 3591 4627 5266 2091
Size: 19 1481 1036 639 5076
Fragments arranged by size:
5076 1481 1036 639 19
EcoRI G'AATT_C
Cuts at: 2987 5560 5938 2987
Size: 2573 378 5300
Fragments arranged by size:
5300 2573 378
EcoRV GAT'ATC
Cuts at: 3509 3509
Size: 8251
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2320 3973 2320
Size: 1653 6598
FspI TGC'GCA
Cuts at: 6757 6757
Size: 8251
HaeII r_GCGC'y
Cuts at: 1678 7398 1678
Size: 5720 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3578 3610 4091 4123 5276
Size: 32 135 32 2006 32 481 32 1153
Cuts at: 5276 5308 5348 5380 6892 6924 6966 6998 1373
Size: 32 40 32 1512 32 42 32 2626
Fragments arranged by size:
2626 2006 1512 1153 481 135 42 40
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5595 5841 669
Size: 988 281 3657 246 3079
Fragments arranged by size:
3657 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2226 3615 748
Size: 1478 1389 5384
Fragments arranged by size:
5384 1478 1389
HpaI GTT'AAC
Cuts at: 5841 5841
Size: 8251
KpnI G_GTAC'C
Cuts at: 2527 5576 2527
Size: 3049 5202
MfeI C'AATT_G
Cuts at: 5850 5850
Size: 8251
MluI A'CGCG_T
Cuts at: 8127 8127
Size: 8251
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7365 7549 1305
Size: 6060 184 2007
Fragments arranged by size:
6060 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3173 5180 10
Size: 54 1425 343 1341 2007 3081
Fragments arranged by size:
3081 2007 1425 1341 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2495 2797 4098 4150 6209 6568 6727
Size: 648 1329 302 1301 52 2059 359 159
Cuts at: 6727 518
Size: 2042
Fragments arranged by size:
2059 2042 1329 1301 648 359 302 159
52
MspA1I CmG'CkG
Cuts at: 1606 2064 2175 2552 2623 4438 4661 4835 5231
Size: 458 111 377 71 1815 223 174 396
Cuts at: 5231 5329 5350 5471 5667 6344 7492 7737 1606
Size: 98 21 121 196 677 1148 245 2120
Fragments arranged by size:
2120 1815 1148 677 458 396 377 245
223 196 174 121 111 98 71 21
NcoI C'CATG_G
Cuts at: 513 1065 2004 2527 2973 513
Size: 552 939 523 446 5791
Fragments arranged by size:
5791 939 552 523 446
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8251
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8251
NotI GC'GGCC_GC
Cuts at: 5698 5698
Size: 8251
NspI r_CATG'y
Cuts at: 1132 1229 3097 3567 3933 4097 4501 5609 7154
Size: 97 1868 470 366 164 404 1108 1545
Cuts at: 7154 1132
Size: 2229
Fragments arranged by size:
2229 1868 1545 1108 470 404 366 164
97
PasI CC'CwG_GG
Cuts at: 1348 2615 1348
Size: 1267 6984
PciI A'CATG_T
Cuts at: 7150 7150
Size: 8251
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4724 1108
Size: 3616 4635
PmeI GTTT'AAAC
Cuts at: 5555 5555
Size: 8251
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6320 6352 7833 7865 6320
Size: 32 1481 32 6706
Fragments arranged by size:
6706 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2110 4627 2110
Size: 2517 5734
PshAI GACnn'nnGTC
Cuts at: 1613 2536 1613
Size: 923 7328
PsiI TTA'TAA
Cuts at: 5821 5821
Size: 8251
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2851 2883 2851
Size: 32 8219
PstI C_TGCA'G
Cuts at: 830 2683 3007 3154 4986 5038 5448 5603 830
Size: 1853 324 147 1832 52 410 155 3478
Fragments arranged by size:
3478 1853 1832 410 324 155 147 52
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8251
PvuII CAG'CTG
Cuts at: 2175 2552 2623 4438 4661 4835 5329 5350 5471
Size: 377 71 1815 223 174 494 21 121
Cuts at: 5471 2175
Size: 4955
Fragments arranged by size:
4955 1815 494 377 223 174 121 71
21
SacI G_AGCT'C
Cuts at: 2294 2294
Size: 8251
SacII CC_GC'GG
Cuts at: 2065 2065
Size: 8251
SalI G'TCGA_C
Cuts at: 1936 5593 1936
Size: 3657 4594
SanDI GG'GwC_CC
Cuts at: 2110 2110
Size: 8251
SapI GCTCTTCn'nnn_
Cuts at: 4587 5010 4587
Size: 423 7828
SbfI CC_TGCA'GG
Cuts at: 5448 5603 5448
Size: 155 8096
ScaI AGT'ACT
Cuts at: 3200 6499 8211 3200
Size: 3299 1712 3240
Fragments arranged by size:
3299 3240 1712
SfcI C'TryA_G
Cuts at: 826 1045 2662 2679 2811 3003 3150 4088 4982
Size: 219 1617 17 132 192 147 938 894
Cuts at: 4982 5010 5034 5444 5453 5599 6734 7415 7606
Size: 28 24 410 9 146 1135 681 191
Cuts at: 7606 826
Size: 1471
Fragments arranged by size:
1617 1471 1135 938 894 681 410 219
192 191 147 146 132 28 24 17
9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8251
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8251
SmaI CCC'GGG
Cuts at: 1872 5485 5578 1872
Size: 3613 93 4545
Fragments arranged by size:
4545 3613 93
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3292 5136 6355 7256 7518 820
Size: 197 926 1349 1844 1219 901 262 1553
Fragments arranged by size:
1844 1553 1349 1219 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6042 493
Size: 5549 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8251
SphI G_CATG'C
Cuts at: 1132 3567 4097 5609 1132
Size: 2435 530 1512 3774
Fragments arranged by size:
3774 2435 1512 530
SspI AAT'ATT
Cuts at: 5 52 6175 5
Size: 47 6123 2081
Fragments arranged by size:
6123 2081 47
StuI AGG'CCT
Cuts at: 1517 3851 1517
Size: 2334 5917
StyI C'CwwG_G
Cuts at: 513 1065 2004 2295 2413 2527 2724 2973 4340
Size: 552 939 291 118 114 197 249 1367
Cuts at: 4340 4668 4755 5690 513
Size: 328 87 935 3074
Fragments arranged by size:
3074 1367 939 935 552 328 291 249
197 118 114 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2168 2461 2809 3913 4162 4224 4828
Size: 1255 588 293 348 1104 249 62 604
Cuts at: 4828 4938 6278 6295 6448 6633 7078 325
Size: 110 1340 17 153 185 445 1498
Fragments arranged by size:
1498 1340 1255 1104 604 588 445 348
293 249 185 153 110 62 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2737 3056 3198
Size: 274 80 33 51 1443 760 319 142
Cuts at: 3198 5521 6497 8209 96
Size: 2323 976 1712 138
Fragments arranged by size:
2323 1712 1443 976 760 319 274 142
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2732 2764 4567 4599 8211 8243 2732
Size: 32 1803 32 3612 32 2740
Fragments arranged by size:
3612 2740 1803 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4450 1316
Size: 3134 5117
XbaI T'CTAG_A
Cuts at: 5587 5587
Size: 8251
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3907 4435 5326 3907
Size: 528 891 6832
Fragments arranged by size:
6832 891 528
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8251
XmaI C'CCGG_G
Cuts at: 1870 5483 5576 1870
Size: 3613 93 4545
Fragments arranged by size:
4545 3613 93
XmnI GAAnn'nnTTC
Cuts at: 3215 4874 6380 3215
Size: 1659 1506 5086
Fragments arranged by size:
5086 1659 1506
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3406 6059 276
Size: 53 83 186 936 1872 2653 2468
Fragments arranged by size:
2653 2468 1872 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI
BsmBI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI RsrII
SexAI SfiI SfoI SrfI SwaI