Restriction map for
pFN21AE3773
(Circular) (Six-base) MAPSORT of: pFN21AE3773.seq Check: 7505 from: 1 to: 9934
pFN21AE3773 9934 bp
With 182 enzymes: SgfI *
March 28, 2012 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6619 6619
Size: 9934
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7744 278
Size: 53 83 186 936 6208 2468
Fragments arranged by size:
6208 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6625 6917 7277 1937
Size: 4688 292 360 4594
Fragments arranged by size:
4688 4594 360 292
Acc65I G'GTAC_C
Cuts at: 7255 7255
Size: 9934
AclI AA'CG_TT
Cuts at: 1563 8061 8434 1563
Size: 6498 373 3063
Fragments arranged by size:
6498 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2852 3153 3648 3843 4235 4658 4935 5223
Size: 1220 301 495 195 392 423 277 288
Cuts at: 5223 5472 6053 6141 6174 7997 9381 9649 1632
Size: 249 581 88 33 1823 1384 268 1917
Fragments arranged by size:
1917 1823 1384 1220 581 495 423 392
301 288 277 268 249 195 88 33
AflII C'TTAA_G
Cuts at: 820 1017 2752 820
Size: 197 1735 8002
Fragments arranged by size:
8002 1735 197
AflIII A'CryG_T
Cuts at: 1203 3640 3967 6676 8833 9810 1203
Size: 2437 327 2709 2157 977 1327
Fragments arranged by size:
2709 2437 2157 1327 977 327
AgeI A'CCGG_T
Cuts at: 8765 8765
Size: 9934
AhdI GACnn_n'nnGTC
Cuts at: 3706 8663 3706
Size: 4957 4977
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9934
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 6751 7224 1184
Size: 5567 473 3894
Fragments arranged by size:
5567 3894 473
AlwNI CAG_nnn'CTG
Cuts at: 3722 5499 7677 9249 3722
Size: 1777 2178 1572 4407
Fragments arranged by size:
4407 2178 1777 1572
ApaLI G'TGCA_C
Cuts at: 4791 7991 9147 4791
Size: 3200 1156 5578
Fragments arranged by size:
5578 3200 1156
ApoI r'AATT_y
Cuts at: 1493 2061 2447 2772 3271 3350 3843 4232 4409
Size: 568 386 325 499 79 493 389 177
Cuts at: 4409 4555 6176 6284 6823 7101 7243 7469 7621
Size: 146 1621 108 539 278 142 226 152
Cuts at: 7621 9730 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1621 568 539 499 493 389
386 325 278 226 177 152 146 142
108 79
AseI AT'TA_AT
Cuts at: 160 1032 8488 160
Size: 872 7456 1606
Fragments arranged by size:
7456 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9934
AvaI C'yCGr_G
Cuts at: 1870 1943 5399 7259 1870
Size: 73 3456 1860 4545
Fragments arranged by size:
4545 3456 1860 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5000 5033 5616 5649 6135 6168 5000
Size: 33 583 33 486 33 8766
Fragments arranged by size:
8766 583 486 33 33 33
BamHI G'GATC_C
Cuts at: 2023 5686 7264 7631 2023
Size: 3663 1578 367 4326
Fragments arranged by size:
4326 3663 1578 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4915 7255 8710 618
Size: 325 217 627 3128 2340 1455 1842
Fragments arranged by size:
3128 2340 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3794 6549 7253 1391
Size: 2403 2755 704 4072
Fragments arranged by size:
4072 2755 2403 704
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3309 3488 928
Size: 971 1410 179 7374
Fragments arranged by size:
7374 1410 971 179
BbvCI CC'TCA_GC
Cuts at: 3222 3561 5892 3222
Size: 339 2331 7264
Fragments arranged by size:
7264 2331 339
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8125 8159 1903
Size: 34 6188 34 3678
Fragments arranged by size:
6188 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3051 3220 4057 5028 5407 7092 7826 9036 3051
Size: 169 837 971 379 1685 734 1210 3949
Fragments arranged by size:
3949 1685 1210 971 837 734 379 169
BclI T'GATC_A
Cuts at: 1548 3634 5116 1548
Size: 2086 1482 6366
Fragments arranged by size:
6366 2086 1482
BfrBI ATG'CAT
Cuts at: 3212 3212
Size: 9934
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5042 8545 136
Size: 107 122 71 631 605 3370 3503 1525
Fragments arranged by size:
3503 3370 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6632 9929 6632
Size: 3297 6637
BlpI GC'TnA_GC
Cuts at: 2562 3597 7351 2562
Size: 1035 3754 5145
Fragments arranged by size:
5145 3754 1035
Bme1580I G_kGCm'C
Cuts at: 1790 4795 7995 9151 1790
Size: 3005 3200 1156 2573
Fragments arranged by size:
3200 3005 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 6623 1318
Size: 5305 4629
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3615 3753 4598
Size: 416 53 83 399 24 2198 138 845
Cuts at: 4598 6658 7665 8623 442
Size: 2060 1007 958 1753
Fragments arranged by size:
2198 2060 1753 1007 958 845 416 399
138 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9934
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5680 5680
Size: 9934
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2591 2940 4287 4313 4509 5333 6732 8594
Size: 1352 349 1347 26 196 824 1399 1862
Cuts at: 8594 1239
Size: 2579
Fragments arranged by size:
2579 1862 1399 1352 1347 824 349 196
26
Bpu10I CC'TnA_GC
Cuts at: 3222 3561 5892 3222
Size: 339 2331 7264
Fragments arranged by size:
7264 2331 339
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5160 8059 8924 9222 5160
Size: 2899 865 298 5872
Fragments arranged by size:
5872 2899 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2609 3453 8597 882
Size: 617 1110 844 5144 2219
Fragments arranged by size:
5144 2219 1110 844 617
BsaAI yAC'GTr
Cuts at: 493 1204 7725 493
Size: 711 6521 2702
Fragments arranged by size:
6521 2702 711
BsaBI GATnn'nnATC
Cuts at: 9928 9928
Size: 9934
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3470 7741 8123 8758
Size: 53 83 186 936 1937 4271 382 635
Cuts at: 8758 275
Size: 1451
Fragments arranged by size:
4271 1937 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3078 8367 8765 9039 9186 9597 3078
Size: 5289 398 274 147 411 3415
Fragments arranged by size:
5289 3415 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2791 2821 4645 4675 6987
Size: 30 165 30 1388 30 1824 30 2312
Cuts at: 6987 7017 7204 7234 1178
Size: 30 187 30 3878
Fragments arranged by size:
3878 2312 1824 1388 187 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2484 2799 3676 4581 4653 1186
Size: 1298 315 877 905 72 6467
Fragments arranged by size:
6467 1298 905 877 315 72
BseYI C'CCAG_C
Cuts at: 5636 6257 6522 9137 9633 5636
Size: 621 265 2615 496 5937
Fragments arranged by size:
5937 2615 621 496 265
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2785 4980 7220 1163
Size: 573 1049 2195 2240 3877
Fragments arranged by size:
3877 2240 2195 1049 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7384 8145 9173 1540
Size: 275 186 5383 761 1028 2301
Fragments arranged by size:
5383 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4795 5379 6549 7995 8080 9151 4795
Size: 584 1170 1446 85 1071 5578
Fragments arranged by size:
5578 1446 1170 1071 584 85
BsmI GAATG_Cn'
Cuts at: 3067 3901 4678 7044 7445 7538 3067
Size: 834 777 2366 401 93 5463
Fragments arranged by size:
5463 2366 834 777 401 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3794 4795 5379 6549 7253 7995 8080
Size: 399 2004 1001 584 1170 704 742 85
Cuts at: 8080 9151 1391
Size: 1071 2174
Fragments arranged by size:
2174 2004 1170 1071 1001 742 704 584
399 85
BspEI T'CCGG_A
Cuts at: 9597 9597
Size: 9934
BspHI T'CATG_A
Cuts at: 2736 7823 9553 2736
Size: 5087 1730 3117
Fragments arranged by size:
5087 3117 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6025 6619 7289 844
Size: 5181 594 670 3489
Fragments arranged by size:
5181 3489 670 594
BsrBI CCG'CTC
Cuts at: 1394 1823 3596 7821 1394
Size: 429 1773 4225 3507
Fragments arranged by size:
4225 3507 1773 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2262 4585 4757 8429 8603 65
Size: 1183 1014 2323 172 3672 174 1396
Fragments arranged by size:
3672 2323 1396 1183 1014 174 172
BsrFI r'CCGG_y
Cuts at: 1700 1810 8578 8765 1700
Size: 110 6768 187 2869
Fragments arranged by size:
6768 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3964 5521 96
Size: 3868 1557 4509
Fragments arranged by size:
4509 3868 1557
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9934
BssSI C'ACGA_G
Cuts at: 7994 9006 7994
Size: 1012 8922
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2794 3218 3722 3928 794
Size: 2000 424 504 206 6800
Fragments arranged by size:
6800 2000 504 424 206
BstBI TT'CG_AA
Cuts at: 4102 6652 8738 4102
Size: 2550 2086 5298
Fragments arranged by size:
5298 2550 2086
BstEII G'GTnAC_C
Cuts at: 1184 4315 1184
Size: 3131 6803
BstXI CCAn_nnnn'nTGG
Cuts at: 4058 6965 7092 4058
Size: 2907 127 6900
Fragments arranged by size:
6900 2907 127
BstYI r'GATC_y
Cuts at: 1972 2023 2827 2867 4452 5686 6218 6632 6703
Size: 51 804 40 1585 1234 532 414 71
Cuts at: 6703 7264 7631 8016 8033 9485 9571 9583 9929
Size: 561 367 385 17 1452 86 12 346
Cuts at: 9929 1972
Size: 1977
Fragments arranged by size:
1977 1585 1452 1234 804 561 532 414
385 367 346 86 71 51 40 17
12
Bsu36I CC'TnA_GG
Cuts at: 3381 3381
Size: 9934
BtgI C'CryG_G
Cuts at: 513 1065 2004 6964 513
Size: 552 939 4960 3483
Fragments arranged by size:
4960 3483 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5858 487
Size: 683 56 4632 4563
Fragments arranged by size:
4632 4563 683 56
BtsI GCAGTG_nn'
Cuts at: 3679 6313 6433 7450 8244 8264 3679
Size: 2634 120 1017 794 20 5349
Fragments arranged by size:
5349 2634 1017 794 120 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9934
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4474 4509 529
Size: 35 3910 35 5954
Fragments arranged by size:
5954 3910 35 35
DraI TTT'AAA
Cuts at: 2479 2731 2778 7238 2479
Size: 252 47 4460 5175
Fragments arranged by size:
5175 4460 252 47
DraIII CAC_nnn'GTG
Cuts at: 6713 6713
Size: 9934
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5803 8941 809
Size: 507 4487 3138 1802
Fragments arranged by size:
4487 3138 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3027 4699
Size: 54 1425 50 275 18 177 1020 1672
Cuts at: 4699 7381 8270 9899 8
Size: 2682 889 1629 43
Fragments arranged by size:
2682 1672 1629 1425 1020 889 275 177
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7381 1537
Size: 275 5569 4090
Fragments arranged by size:
5569 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2165 2461 4086 4192 4677 5751 6072 6404
Size: 812 296 1625 106 485 1074 321 332
Cuts at: 6404 7864 1353
Size: 1460 3423
Fragments arranged by size:
3423 1625 1460 1074 812 485 332 321
296 106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8521 8895 9041 387
Size: 1064 138 6932 374 146 1280
Fragments arranged by size:
6932 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 6547 6547
Size: 9934
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2591 2852 2940 3153 3648 3843 4235
Size: 393 959 261 88 213 495 195 392
Cuts at: 4235 4287 4313 4509 4658 4935 5223 5333 5472
Size: 52 26 196 149 277 288 110 139
Cuts at: 5472 6053 6141 6174 6732 7997 8594 9381 9649
Size: 581 88 33 558 1265 597 787 268
Cuts at: 9649 1239
Size: 1524
Fragments arranged by size:
1524 1265 959 787 597 581 558 495
393 392 288 277 268 261 213 196
195 149 139 110 88 88 52 33
26
EcoO109I rG'GnC_Cy
Cuts at: 2703 2858 3196 4027 5611 2703
Size: 155 338 831 1584 7026
Fragments arranged by size:
7026 1584 831 338 155
EcoRI G'AATT_C
Cuts at: 7243 7621 7243
Size: 378 9556
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2734 4097 2734
Size: 1363 8571
FspI TGC'GCA
Cuts at: 8440 8440
Size: 9934
HaeII r_GCGC'y
Cuts at: 1678 9081 1678
Size: 7403 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2718 2750 2869 2901 3251
Size: 32 135 32 1146 32 119 32 350
Cuts at: 3251 3283 3692 3724 3778 3810 4611 4643 4645
Size: 32 409 32 54 32 801 32 2
Cuts at: 4645 4675 4677 5447 5479 5750 5782 6422 6454
Size: 30 2 770 32 271 32 640 32
Cuts at: 6454 6934 6966 6987 7019 8575 8607 8649 8681
Size: 480 32 21 32 1556 32 42 32
Cuts at: 8681 1373
Size: 2626
Fragments arranged by size:
2626 1556 1146 801 770 640 480 409
350 271 135 119 54 42 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 30 21 2
2
HincII GTy'rAC
Cuts at: 669 1657 1938 2340 2553 6626 6866 7278 7524
Size: 988 281 402 213 4073 240 412 246
Cuts at: 7524 669
Size: 3079
Fragments arranged by size:
4073 3079 988 412 402 281 246 240
213
HindIII A'AGCT_T
Cuts at: 748 2279 4105 5555 6372 748
Size: 1531 1826 1450 817 4310
Fragments arranged by size:
4310 1826 1531 1450 817
HpaI GTT'AAC
Cuts at: 2553 7524 2553
Size: 4971 4963
KpnI G_GTAC'C
Cuts at: 7259 7259
Size: 9934
MfeI C'AATT_G
Cuts at: 5772 7533 5772
Size: 1761 8173
MluI A'CGCG_T
Cuts at: 9810 9810
Size: 9934
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4039 4539 5563 7211 9048 9232 1305
Size: 2734 500 1024 1648 1837 184 2007
Fragments arranged by size:
2734 2007 1837 1648 1024 500 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 3029 10
Size: 54 1425 343 177 1020 6915
Fragments arranged by size:
6915 1425 1020 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2903 2979 3426 4821 5012 5939 5964
Size: 648 1737 76 447 1395 191 927 25
Cuts at: 5964 6937 7892 8251 8410 518
Size: 973 955 359 159 2042
Fragments arranged by size:
2042 1737 1395 973 955 927 648 447
359 191 159 76 25
MspA1I CmG'CkG
Cuts at: 1606 4291 4970 5813 5863 7350 8027 9175 9420
Size: 2685 679 843 50 1487 677 1148 245
Cuts at: 9420 1606
Size: 2120
Fragments arranged by size:
2685 2120 1487 1148 843 679 677 245
50
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 8443
Fragments arranged by size:
8443 939 552
NdeI CA'TA_TG
Cuts at: 387 3394 7033 387
Size: 3007 3639 3288
Fragments arranged by size:
3639 3288 3007
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9934
NotI GC'GGCC_GC
Cuts at: 7381 7381
Size: 9934
NsiI A_TGCA'T
Cuts at: 3214 3214
Size: 9934
NspI r_CATG'y
Cuts at: 1132 1229 3644 3971 6211 6680 7292 8837 1132
Size: 97 2415 327 2240 469 612 1545 2229
Fragments arranged by size:
2415 2240 2229 1545 612 469 327 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9934
PciI A'CATG_T
Cuts at: 3640 3967 6676 8833 3640
Size: 327 2709 2157 4741
Fragments arranged by size:
4741 2709 2157 327
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 9934
PfoI T'CCnGG_A
Cuts at: 5280 5280
Size: 9934
PmeI GTTT'AAAC
Cuts at: 7238 7238
Size: 9934
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8003 8035 9516 9548 8003
Size: 32 1481 32 8389
Fragments arranged by size:
8389 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2703 2858 3196 4027 5611 2703
Size: 155 338 831 1584 7026
Fragments arranged by size:
7026 1584 831 338 155
PshAI GACnn'nnGTC
Cuts at: 1613 5480 1613
Size: 3867 6067
PsiI TTA'TAA
Cuts at: 7504 7504
Size: 9934
PstI C_TGCA'G
Cuts at: 830 2541 5739 6543 7286 830
Size: 1711 3198 804 743 3478
Fragments arranged by size:
3478 3198 1711 804 743
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9934
PvuII CAG'CTG
Cuts at: 4291 4970 5813 4291
Size: 679 843 8412
Fragments arranged by size:
8412 843 679
SacI G_AGCT'C
Cuts at: 6549 6549
Size: 9934
SalI G'TCGA_C
Cuts at: 1936 6624 7276 1936
Size: 4688 652 4594
Fragments arranged by size:
4688 4594 652
SanDI GG'GwC_CC
Cuts at: 2858 2858
Size: 9934
SapI GCTCTTCn'nnn_
Cuts at: 4086 4192 6072 4086
Size: 106 1880 7948
Fragments arranged by size:
7948 1880 106
SbfI CC_TGCA'GG
Cuts at: 7286 7286
Size: 9934
ScaI AGT'ACT
Cuts at: 8182 9894 8182
Size: 1712 8222
SfcI C'TryA_G
Cuts at: 826 1045 2160 2415 2537 4767 5190 5735 6539
Size: 219 1115 255 122 2230 423 545 804
Cuts at: 6539 6699 7125 7282 8417 9098 9289 826
Size: 160 426 157 1135 681 191 1471
Fragments arranged by size:
2230 1471 1135 1115 804 681 545 426
423 255 219 191 160 157 122
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9934
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9934
SmaI CCC'GGG
Cuts at: 1872 7261 1872
Size: 5389 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2752 5175 8038 8939 9201 820
Size: 197 926 809 2423 2863 901 262 1553
Fragments arranged by size:
2863 2423 1553 926 901 809 262 197
SnaBI TAC'GTA
Cuts at: 493 7725 493
Size: 7232 2702
SpeI A'CTAG_T
Cuts at: 152 3089 3862 152
Size: 2937 773 6224
Fragments arranged by size:
6224 2937 773
SphI G_CATG'C
Cuts at: 1132 6211 7292 1132
Size: 5079 1081 3774
Fragments arranged by size:
5079 3774 1081
SspI AAT'ATT
Cuts at: 5 52 2263 2845 7858 5
Size: 47 2211 582 5013 2081
Fragments arranged by size:
5013 2211 2081 582 47
StuI AGG'CCT
Cuts at: 1517 5696 1517
Size: 4179 5755
StyI C'CwwG_G
Cuts at: 513 1065 2004 3973 7373 513
Size: 552 939 1969 3400 3074
Fragments arranged by size:
3400 3074 1969 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2559 3994 6552 7961 7978 8131 8316
Size: 1255 979 1435 2558 1409 17 153 185
Cuts at: 8316 8761 325
Size: 445 1498
Fragments arranged by size:
2558 1498 1435 1409 1255 979 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3964 4546 5140
Size: 274 80 33 51 1443 1987 582 594
Cuts at: 5140 5521 7087 8180 9892 96
Size: 381 1566 1093 1712 138
Fragments arranged by size:
1987 1712 1566 1443 1093 594 582 381
274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2791 2819 2823 2851 3225 3257 3518 3550 6013
Size: 28 4 28 374 32 261 32 2463
Cuts at: 6013 6045 6303 6335 7204 7236 9894 9926 2791
Size: 32 258 32 869 32 2658 32 2799
Fragments arranged by size:
2799 2658 2463 869 374 261 258 32
32 32 32 32 32 28 28 4
Tth111I GACn'n_nGTC
Cuts at: 1316 4531 4657 1316
Size: 3215 126 6593
Fragments arranged by size:
6593 3215 126
XbaI T'CTAG_A
Cuts at: 7270 7270
Size: 9934
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4611 5278 4611
Size: 667 9267
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9934
XmaI C'CCGG_G
Cuts at: 1870 7259 1870
Size: 5389 4545
XmnI GAAnn'nnTTC
Cuts at: 3554 4201 6558 8063 3554
Size: 647 2357 1505 5425
Fragments arranged by size:
5425 2357 1505 647
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7742 276
Size: 53 83 186 936 6208 2468
Fragments arranged by size:
6208 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BsiWI BsmBI
BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII
SacII SexAI SfiI SfoI SrfI SwaI