Restriction map for
pFN21AE3772
(Circular) (Six-base) MAPSORT of: pFN21AE3772.seq Check: 8826 from: 1 to: 9523
pFN21AE3772 9523 bp
With 182 enzymes: SgfI *
July 6, 2012 17:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2738 5936 2738
Size: 3198 6325
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7333 278
Size: 53 83 186 936 5797 2468
Fragments arranged by size:
5797 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6866 1937
Size: 4929 4594
Acc65I G'GTAC_C
Cuts at: 2883 6844 2883
Size: 3961 5562
AclI AA'CG_TT
Cuts at: 1563 7650 8023 1563
Size: 6087 373 3063
Fragments arranged by size:
6087 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2576 3063 4525 4631 4754 4983 5087 7586
Size: 944 487 1462 106 123 229 104 2499
Cuts at: 7586 8970 9238 1632
Size: 1384 268 1917
Fragments arranged by size:
2499 1917 1462 1384 944 487 268 229
123 106 104
AflII C'TTAA_G
Cuts at: 820 1017 5440 820
Size: 197 4423 4903
Fragments arranged by size:
4903 4423 197
AflIII A'CryG_T
Cuts at: 1203 4216 8422 9399 1203
Size: 3013 4206 977 1327
Fragments arranged by size:
4206 3013 1327 977
AgeI A'CCGG_T
Cuts at: 8354 8354
Size: 9523
AhdI GACnn_n'nnGTC
Cuts at: 5155 8252 5155
Size: 3097 6426
AleI CACnn'nnGTG
Cuts at: 1166 2912 4794 1166
Size: 1746 1882 5895
Fragments arranged by size:
5895 1882 1746
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2756 6617 1184
Size: 1572 3861 4090
Fragments arranged by size:
4090 3861 1572
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3397 3429 6705 6737 3397
Size: 32 3276 32 6183
Fragments arranged by size:
6183 3276 32 32
AlwNI CAG_nnn'CTG
Cuts at: 4136 5089 5334 5376 7266 8838 4136
Size: 953 245 42 1890 1572 4821
Fragments arranged by size:
4821 1890 1572 953 245 42
ApaLI G'TGCA_C
Cuts at: 3299 6610 7580 8736 3299
Size: 3311 970 1156 4086
Fragments arranged by size:
4086 3311 1156 970
ApoI r'AATT_y
Cuts at: 1493 2261 2766 3245 3662 4041 4385 4615 4655
Size: 768 505 479 417 379 344 230 40
Cuts at: 4655 5350 5600 5717 6210 6287 6373 6739 6832
Size: 695 250 117 493 77 86 366 93
Cuts at: 6832 7058 7210 9319 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 768 695 505 493 479 417
379 366 344 250 230 226 152 117
93 86 77 40
AseI AT'TA_AT
Cuts at: 160 1032 3921 8077 160
Size: 872 2889 4156 1606
Fragments arranged by size:
4156 2889 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9523
AvaI C'yCGr_G
Cuts at: 1870 1943 6848 1870
Size: 73 4905 4545
Fragments arranged by size:
4905 4545 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2867 2900 3114 3147 6006 6039 2867
Size: 33 214 33 2859 33 6351
Fragments arranged by size:
6351 2859 214 33 33 33
BamHI G'GATC_C
Cuts at: 6853 7220 6853
Size: 367 9156
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2883 2942 6569 6844 8299
Size: 325 217 627 1096 59 3627 275 1455
Cuts at: 8299 618
Size: 1842
Fragments arranged by size:
3627 1842 1455 1096 627 325 275 217
59
BanII G_rGCy'C
Cuts at: 1391 2097 4530 4955 6842 1391
Size: 706 2433 425 1887 4072
Fragments arranged by size:
4072 2433 1887 706 425
BbeI G_GCGC'C
Cuts at: 6573 6573
Size: 9523
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2108 2244 3461 3834 4133 4308 5064
Size: 971 209 136 1217 373 299 175 756
Cuts at: 5064 5151 928
Size: 87 5300
Fragments arranged by size:
5300 1217 971 756 373 299 209 175
136 87
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2285 2319 3880 3914 7714 7748 1903
Size: 34 348 34 1561 34 3800 34 3678
Fragments arranged by size:
3800 3678 1561 348 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7415 8625 7415
Size: 1210 8313
BclI T'GATC_A
Cuts at: 1548 3189 1548
Size: 1641 7882
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8134 136
Size: 107 122 71 631 605 6462 1525
Fragments arranged by size:
6462 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 9518 9518
Size: 9523
BlpI GC'TnA_GC
Cuts at: 2087 6940 2087
Size: 4853 4670
Bme1580I G_kGCm'C
Cuts at: 1790 2126 3303 4666 6614 7584 8740 1790
Size: 336 1177 1363 1948 970 1156 2573
Fragments arranged by size:
2573 1948 1363 1177 1156 970 336
BmgBI CAC'GTC
Cuts at: 1318 3436 1318
Size: 2118 7405
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 7254 8212 442
Size: 416 53 83 399 24 5837 958 1753
Fragments arranged by size:
5837 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9523
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 2081 2150 3471 4353 5565 6650 6834
Size: 836 6 69 1321 882 1212 1085 184
Cuts at: 6834 8183 1239
Size: 1349 2579
Fragments arranged by size:
2579 1349 1321 1212 1085 882 836 184
69 6
Bpu10I CC'TnA_GC
Cuts at: 6347 6617 6347
Size: 270 9253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4281 5510 7648 8513 8811 4281
Size: 1229 2138 865 298 4993
Fragments arranged by size:
4993 2138 1229 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3937 5039 6747 8186 882
Size: 617 2438 1102 1708 1439 2219
Fragments arranged by size:
2438 2219 1708 1439 1102 617
BsaAI yAC'GTr
Cuts at: 493 1204 7314 493
Size: 711 6110 2702
Fragments arranged by size:
6110 2702 711
BsaBI GATnn'nnATC
Cuts at: 2877 3354 6159 9517 2877
Size: 477 2805 3358 2883
Fragments arranged by size:
3358 2883 2805 477
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6570 7330 7712 8347
Size: 53 83 186 936 5037 760 382 635
Cuts at: 8347 275
Size: 1451
Fragments arranged by size:
5037 1451 936 760 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2430 5691 6756 7956 8354 8628 8775 9186 2430
Size: 3261 1065 1200 398 274 147 411 2767
Fragments arranged by size:
3261 2767 1200 1065 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5158 5188 6705 6735 6807
Size: 30 165 30 3755 30 1517 30 72
Cuts at: 6807 6837 1178
Size: 30 3864
Fragments arranged by size:
3864 3755 1517 165 72 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 6213 1186
Size: 5027 4496
BseYI C'CCAG_C
Cuts at: 2703 5092 5681 6565 6808 8726 9222 2703
Size: 2389 589 884 243 1918 496 3004
Fragments arranged by size:
3004 2389 1918 884 589 496 243
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2193 2716 3193 4772 5094 5410 6610
Size: 573 457 523 477 1579 322 316 1200
Cuts at: 6610 6756 1163
Size: 146 3930
Fragments arranged by size:
3930 1579 1200 573 523 477 457 322
316 146
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6973 7734 8762 1540
Size: 275 186 4972 761 1028 2301
Fragments arranged by size:
4972 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2088 2097 3303 4019 4297 6614 7584 7669 8740
Size: 9 1206 716 278 2317 970 85 1071
Cuts at: 8740 2088
Size: 2871
Fragments arranged by size:
2871 2317 1206 1071 970 716 278 85
9
BsmI GAATG_Cn'
Cuts at: 2147 2288 5947 6353 7034 7127 2147
Size: 141 3659 406 681 93 4543
Fragments arranged by size:
4543 3659 681 406 141 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2097 2126 3303 4019 4297 4530
Size: 399 298 9 29 1177 716 278 233
Cuts at: 4530 4666 4955 6614 6842 7584 7669 8740 1391
Size: 136 289 1659 228 742 85 1071 2174
Fragments arranged by size:
2174 1659 1177 1071 742 716 399 298
289 278 233 228 136 85 29 9
BspEI T'CCGG_A
Cuts at: 2430 5691 9186 2430
Size: 3261 3495 2767
Fragments arranged by size:
3495 3261 2767
BspHI T'CATG_A
Cuts at: 3132 4912 5299 5458 7412 9142 3132
Size: 1780 387 159 1954 1730 3513
Fragments arranged by size:
3513 1954 1780 1730 387 159
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2333 2738 5936 6878 844
Size: 1489 405 3198 942 3489
Fragments arranged by size:
3489 3198 1489 942 405
BsrBI CCG'CTC
Cuts at: 1394 1823 2110 7410 1394
Size: 429 287 5300 3507
Fragments arranged by size:
5300 3507 429 287
BsrDI GCAATG_nn'
Cuts at: 65 1248 3606 4209 4510 8018 8192 65
Size: 1183 2358 603 301 3508 174 1396
Fragments arranged by size:
3508 2358 1396 1183 603 301 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 8167 8354 1700
Size: 110 6357 187 2869
Fragments arranged by size:
6357 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4594 4962 6783 96
Size: 4498 368 1821 2836
Fragments arranged by size:
4498 2836 1821 368
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9523
BssSI C'ACGA_G
Cuts at: 4725 7583 8595 4725
Size: 2858 1012 5653
Fragments arranged by size:
5653 2858 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4349 4500 5388 794
Size: 3555 151 888 4929
Fragments arranged by size:
4929 3555 888 151
BstBI TT'CG_AA
Cuts at: 8327 8327
Size: 9523
BstEII G'GTnAC_C
Cuts at: 1184 3926 1184
Size: 2742 6781
BstXI CCAn_nnnn'nTGG
Cuts at: 2053 2963 6646 2053
Size: 910 3683 4930
Fragments arranged by size:
4930 3683 910
BstYI r'GATC_y
Cuts at: 1972 3869 4074 5665 6476 6853 7220 7605 7622
Size: 1897 205 1591 811 377 367 385 17
Cuts at: 7622 9074 9160 9172 9518 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1897 1591 1452 811 385 377 367
346 205 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3288 3288
Size: 9523
BtgI C'CryG_G
Cuts at: 513 1065 2020 2567 4986 5029 6658 513
Size: 552 955 547 2419 43 1629 3378
Fragments arranged by size:
3378 2419 1629 955 552 547 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5341 487
Size: 683 56 4115 4669
Fragments arranged by size:
4669 4115 683 56
BtsI GCAGTG_nn'
Cuts at: 2598 2968 4678 4784 4801 6520 7039 7833 7853
Size: 370 1710 106 17 1719 519 794 20
Cuts at: 7853 2598
Size: 4268
Fragments arranged by size:
4268 1719 1710 794 519 370 106 20
17
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9523
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9488
DraI TTT'AAA
Cuts at: 2341 3102 4759 6061 6208 6827 2341
Size: 761 1657 1302 147 619 5037
Fragments arranged by size:
5037 1657 1302 761 619 147
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6766 8530 809
Size: 507 5450 1764 1802
Fragments arranged by size:
5450 1802 1764 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2896 3540 4244
Size: 54 1425 50 275 18 1066 644 704
Cuts at: 4244 4398 5026 5820 6648 6970 7859 9488 8
Size: 154 628 794 828 322 889 1629 43
Fragments arranged by size:
1629 1425 1066 889 828 794 704 644
628 322 275 154 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6970 1537
Size: 275 5158 4090
Fragments arranged by size:
5158 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3039 3588 4108 4365 5721 5760 6066 7453
Size: 1686 549 520 257 1356 39 306 1387
Cuts at: 7453 1353
Size: 3423
Fragments arranged by size:
3423 1686 1387 1356 549 520 306 257
39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3106 8110 8484 8630 387
Size: 1064 138 1517 5004 374 146 1280
Fragments arranged by size:
5004 1517 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2095 2095
Size: 9523
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2081 2150 2576 3063 3471 4353
Size: 393 443 6 69 426 487 408 882
Cuts at: 4353 4525 4631 4754 4983 5087 5565 6650 6834
Size: 172 106 123 229 104 478 1085 184
Cuts at: 6834 7586 8183 8970 9238 1239
Size: 752 597 787 268 1524
Fragments arranged by size:
1524 1085 882 787 752 597 487 478
443 426 408 393 268 229 184 172
123 106 104 69 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3291 3291
Size: 9523
EcoO109I rG'GnC_Cy
Cuts at: 2050 3519 4743 5553 2050
Size: 1469 1224 810 6020
Fragments arranged by size:
6020 1469 1224 810
EcoRI G'AATT_C
Cuts at: 2261 3662 5350 6832 7210 2261
Size: 1401 1688 1482 378 4574
Fragments arranged by size:
4574 1688 1482 1401 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2261 4322 6067 2261
Size: 2061 1745 5717
Fragments arranged by size:
5717 2061 1745
FspI TGC'GCA
Cuts at: 8029 8029
Size: 9523
HaeII r_GCGC'y
Cuts at: 1678 2046 6573 8670 1678
Size: 368 4527 2097 2531
Fragments arranged by size:
4527 2531 2097 368
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5141 5173 5686 5718 8164
Size: 32 135 32 3569 32 513 32 2446
Cuts at: 8164 8196 8238 8270 1373
Size: 32 42 32 2626
Fragments arranged by size:
3569 2626 2446 513 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2188 6867 7113 669
Size: 988 281 250 4679 246 3079
Fragments arranged by size:
4679 3079 988 281 250 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9523
HpaI GTT'AAC
Cuts at: 7113 7113
Size: 9523
KasI G'GCGC_C
Cuts at: 6569 6569
Size: 9523
KpnI G_GTAC'C
Cuts at: 2887 6848 2887
Size: 3961 5562
MfeI C'AATT_G
Cuts at: 7122 7122
Size: 9523
MluI A'CGCG_T
Cuts at: 9399 9399
Size: 9523
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2655 2739 3243 3595 4869 6508 8637 8821
Size: 1350 84 504 352 1274 1639 2129 184
Cuts at: 8821 1305
Size: 2007
Fragments arranged by size:
2129 2007 1639 1350 1274 504 352 184
84
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3542 4400 5028 5822 6650
Size: 54 1425 343 1710 858 628 794 828
Cuts at: 6650 10
Size: 2883
Fragments arranged by size:
2883 1710 1425 858 828 794 628 343
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2738 2912 2961 4203 4794 4824 4985
Size: 648 1572 174 49 1242 591 30 161
Cuts at: 4985 5409 6584 7481 7840 7999 518
Size: 424 1175 897 359 159 2042
Fragments arranged by size:
2042 1572 1242 1175 897 648 591 424
359 174 161 159 49 30
MspA1I CmG'CkG
Cuts at: 1606 2022 2948 4055 4136 4490 6491 6597 6812
Size: 416 926 1107 81 354 2001 106 215
Cuts at: 6812 6939 7616 8764 9009 1606
Size: 127 677 1148 245 2120
Fragments arranged by size:
2120 2001 1148 1107 926 677 416 354
245 215 127 106 81
NarI GG'CG_CC
Cuts at: 6570 6570
Size: 9523
NcoI C'CATG_G
Cuts at: 513 1065 4986 5029 513
Size: 552 3921 43 5007
Fragments arranged by size:
5007 3921 552 43
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9523
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9523
NotI GC'GGCC_GC
Cuts at: 6970 6970
Size: 9523
NspI r_CATG'y
Cuts at: 1132 1229 2455 4106 4202 4427 6881 8426 1132
Size: 97 1226 1651 96 225 2454 1545 2229
Fragments arranged by size:
2454 2229 1651 1545 1226 225 97 96
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9523
PciI A'CATG_T
Cuts at: 8422 8422
Size: 9523
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4269 1108
Size: 3161 6362
PmeI GTTT'AAAC
Cuts at: 6827 6827
Size: 9523
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6705 6737 7592 7624 9105 9137 6705
Size: 32 855 32 1481 32 7091
Fragments arranged by size:
7091 1481 855 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2050 3519 2050
Size: 1469 8054
PshAI GACnn'nnGTC
Cuts at: 1613 6535 1613
Size: 4922 4601
PsiI TTA'TAA
Cuts at: 3162 4278 4713 7093 3162
Size: 1116 435 2380 5592
Fragments arranged by size:
5592 2380 1116 435
PstI C_TGCA'G
Cuts at: 830 4132 4232 5381 6875 830
Size: 3302 100 1149 1494 3478
Fragments arranged by size:
3478 3302 1494 1149 100
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9523
PvuII CAG'CTG
Cuts at: 4055 4136 4490 6491 6812 4055
Size: 81 354 2001 321 6766
Fragments arranged by size:
6766 2001 354 321 81
SacI G_AGCT'C
Cuts at: 2097 2097
Size: 9523
SacII CC_GC'GG
Cuts at: 2023 2023
Size: 9523
SalI G'TCGA_C
Cuts at: 1936 6865 1936
Size: 4929 4594
SapI GCTCTTCn'nnn_
Cuts at: 5760 5760
Size: 9523
SbfI CC_TGCA'GG
Cuts at: 4232 6875 4232
Size: 2643 6880
ScaI AGT'ACT
Cuts at: 7771 9483 7771
Size: 1712 7811
SfcI C'TryA_G
Cuts at: 826 1045 2868 4107 4128 4228 5377 6690 6871
Size: 219 1823 1239 21 100 1149 1313 181
Cuts at: 6871 8006 8687 8878 826
Size: 1135 681 191 1471
Fragments arranged by size:
1823 1471 1313 1239 1149 1135 681 219
191 181 100 21
SfoI GGC'GCC
Cuts at: 6571 6571
Size: 9523
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9523
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9523
SmaI CCC'GGG
Cuts at: 1872 6850 1872
Size: 4978 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4296 5440 5489 7627 8528 8790
Size: 197 926 2353 1144 49 2138 901 262
Cuts at: 8790 820
Size: 1553
Fragments arranged by size:
2353 2138 1553 1144 926 901 262 197
49
SnaBI TAC'GTA
Cuts at: 493 7314 493
Size: 6821 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9523
SphI G_CATG'C
Cuts at: 1132 2455 4106 6881 1132
Size: 1323 1651 2775 3774
Fragments arranged by size:
3774 2775 1651 1323
SspI AAT'ATT
Cuts at: 5 52 2369 2782 5765 7447 5
Size: 47 2317 413 2983 1682 2081
Fragments arranged by size:
2983 2317 2081 1682 413 47
StuI AGG'CCT
Cuts at: 1517 3286 6427 1517
Size: 1769 3141 4613
Fragments arranged by size:
4613 3141 1769
StyI C'CwwG_G
Cuts at: 513 1065 2046 2487 4986 5029 5669 6776 6962
Size: 552 981 441 2499 43 640 1107 186
Cuts at: 6962 513
Size: 3074
Fragments arranged by size:
3074 2499 1107 981 640 552 441 186
43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2120 3213 7550 7567 7720 7905 8350
Size: 1255 540 1093 4337 17 153 185 445
Cuts at: 8350 325
Size: 1498
Fragments arranged by size:
4337 1498 1255 1093 540 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2251 2823 4236
Size: 274 80 33 51 1443 274 572 1413
Cuts at: 4236 4594 4729 4962 6333 6783 7769 9481 96
Size: 358 135 233 1371 450 986 1712 138
Fragments arranged by size:
1712 1443 1413 1371 986 572 450 358
274 274 233 138 135 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2467 2499 2563 2595 9483 9515 2467
Size: 32 64 32 6888 32 2475
Fragments arranged by size:
6888 2475 64 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4445 1316
Size: 3129 6394
XbaI T'CTAG_A
Cuts at: 6859 6859
Size: 9523
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2653 4457 4984 2653
Size: 1804 527 7192
Fragments arranged by size:
7192 1804 527
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9523
XmaI C'CCGG_G
Cuts at: 1870 6848 1870
Size: 4978 4545
XmnI GAAnn'nnTTC
Cuts at: 4305 4632 6174 6288 7652 4305
Size: 327 1542 114 1364 6176
Fragments arranged by size:
6176 1542 1364 327 114
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7331 276
Size: 53 83 186 936 5797 2468
Fragments arranged by size:
5797 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbvCI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SanDI SexAI SfiI SrfI SwaI