Restriction map for
pFN21AE3769
(Circular) (Six-base) MAPSORT of: pFN21AE3769.seq Check: 7911 from: 1 to: 7453
pFN21AE3769 7453 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2801 2801
Size: 7453
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5263 278
Size: 53 83 186 936 3727 2468
Fragments arranged by size:
3727 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3609 4796 1937
Size: 1672 1187 4594
Fragments arranged by size:
4594 1672 1187
Acc65I G'GTAC_C
Cuts at: 4774 4774
Size: 7453
AclI AA'CG_TT
Cuts at: 1563 4342 4752 5580 5953 1563
Size: 2779 410 828 373 3063
Fragments arranged by size:
3063 2779 828 410 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2444 3143 3598 3973 5516 6900 7168 1632
Size: 812 699 455 375 1543 1384 268 1917
Fragments arranged by size:
1917 1543 1384 812 699 455 375 268
AfeI AGC'GCT
Cuts at: 3660 3660
Size: 7453
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7256
AflIII A'CryG_T
Cuts at: 1203 2169 4413 4566 6352 7329 1203
Size: 966 2244 153 1786 977 1327
Fragments arranged by size:
2244 1786 1327 977 966 153
AgeI A'CCGG_T
Cuts at: 6284 6284
Size: 7453
AhdI GACnn_n'nnGTC
Cuts at: 4306 6182 4306
Size: 1876 5577
AleI CACnn'nnGTG
Cuts at: 1166 2453 3575 1166
Size: 1287 1122 5044
Fragments arranged by size:
5044 1287 1122
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7453
AlwNI CAG_nnn'CTG
Cuts at: 2237 3052 4362 5196 6768 2237
Size: 815 1310 834 1572 2922
Fragments arranged by size:
2922 1572 1310 834 815
ApaLI G'TGCA_C
Cuts at: 5510 6666 5510
Size: 1156 6297
ApoI r'AATT_y
Cuts at: 1493 3498 4024 4391 4640 4762 4988 5140 7249
Size: 2005 526 367 249 122 226 152 2109
Cuts at: 7249 1493
Size: 1697
Fragments arranged by size:
2109 2005 1697 526 367 249 226 152
122
AseI AT'TA_AT
Cuts at: 160 1032 6007 160
Size: 872 4975 1606
Fragments arranged by size:
4975 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7453
AvaI C'yCGr_G
Cuts at: 1870 1943 3537 4778 1870
Size: 73 1594 1241 4545
Fragments arranged by size:
4545 1594 1241 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2317 2350 2317
Size: 33 7420
BamHI G'GATC_C
Cuts at: 4783 5150 4783
Size: 367 7086
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2974 4774 6229 618
Size: 325 217 627 1187 1800 1455 1842
Fragments arranged by size:
1842 1800 1455 1187 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2766 4215 4772 1391
Size: 1375 1449 557 4072
Fragments arranged by size:
4072 1449 1375 557
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4187 928
Size: 971 2288 4194
Fragments arranged by size:
4194 2288 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4448 4482 5644 5678 1903
Size: 34 2511 34 1162 34 3678
Fragments arranged by size:
3678 2511 1162 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3202 5345 6555 3202
Size: 2143 1210 4100
Fragments arranged by size:
4100 2143 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7453
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2972 6064 136
Size: 107 122 71 631 605 1300 3092 1525
Fragments arranged by size:
3092 1525 1300 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3085 7448 3085
Size: 4363 3090
BlpI GC'TnA_GC
Cuts at: 4870 4870
Size: 7453
Bme1580I G_kGCm'C
Cuts at: 1790 5514 6670 1790
Size: 3724 1156 2573
Fragments arranged by size:
3724 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7453
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3924 5184 6142
Size: 416 53 83 399 24 2507 1260 958
Cuts at: 6142 442
Size: 1753
Fragments arranged by size:
2507 1753 1260 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7453
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3445 3445
Size: 7453
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3415 3802 4384 6113 1239
Size: 2176 387 582 1729 2579
Fragments arranged by size:
2579 2176 1729 582 387
Bpu10I CC'TnA_GC
Cuts at: 3258 3258
Size: 7453
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2979 3731 5578 6443 6741 2979
Size: 752 1847 865 298 3691
Fragments arranged by size:
3691 1847 865 752 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3929 4223 6116 882
Size: 617 2430 294 1893 2219
Fragments arranged by size:
2430 2219 1893 617 294
BsaAI yAC'GTr
Cuts at: 493 1204 5244 493
Size: 711 4040 2702
Fragments arranged by size:
4040 2702 711
BsaBI GATnn'nnATC
Cuts at: 7447 7447
Size: 7453
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2964 5260 5642 6277
Size: 53 83 186 936 1431 2296 382 635
Cuts at: 6277 275
Size: 1451
Fragments arranged by size:
2296 1451 1431 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2035 5886 6284 6558 6705 7116 2035
Size: 3851 398 274 147 411 2372
Fragments arranged by size:
3851 2372 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3611 3641 1178
Size: 30 165 30 2208 30 4990
Fragments arranged by size:
4990 2208 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3003 1186
Size: 1817 5636
BseYI C'CCAG_C
Cuts at: 2163 2618 6656 7152 2163
Size: 455 4038 496 2464
Fragments arranged by size:
4038 2464 496 455
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2817 1163
Size: 573 1081 5799
Fragments arranged by size:
5799 1081 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2036 4903 5664 6692 1540
Size: 275 186 35 2867 761 1028 2301
Fragments arranged by size:
2867 2301 1028 761 275 186 35
BsiHKAI G_wGCw'C
Cuts at: 4215 5514 5599 6670 4215
Size: 1299 85 1071 4998
Fragments arranged by size:
4998 1299 1071 85
BsmI GAATG_Cn'
Cuts at: 4964 5057 4964
Size: 93 7360
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2766 4215 4772 5514 5599 6670 1391
Size: 399 976 1449 557 742 85 1071 2174
Fragments arranged by size:
2174 1449 1071 976 742 557 399 85
BspEI T'CCGG_A
Cuts at: 7116 7116
Size: 7453
BspHI T'CATG_A
Cuts at: 2799 5342 7072 2799
Size: 2543 1730 3180
Fragments arranged by size:
3180 2543 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2801 4808 844
Size: 1957 2007 3489
Fragments arranged by size:
3489 2007 1957
BsrBI CCG'CTC
Cuts at: 1394 1823 5340 1394
Size: 429 3517 3507
Fragments arranged by size:
3517 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2487 3444 3817 4464 5948 6122 65
Size: 1183 1239 957 373 647 1484 174 1396
Fragments arranged by size:
1484 1396 1239 1183 957 647 373 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2121 6097 6284 1700
Size: 110 311 3976 187 2869
Fragments arranged by size:
3976 2869 311 187 110
BsrGI T'GTAC_A
Cuts at: 96 2172 96
Size: 2076 5377
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7453
BssSI C'ACGA_G
Cuts at: 5513 6525 5513
Size: 1012 6441
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2689 794
Size: 1895 5558
BstBI TT'CG_AA
Cuts at: 6257 6257
Size: 7453
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7453
BstXI CCAn_nnnn'nTGG
Cuts at: 2618 4324 2618
Size: 1706 5747
BstYI r'GATC_y
Cuts at: 1972 2233 2702 3085 4320 4783 5150 5535 5552
Size: 261 469 383 1235 463 367 385 17
Cuts at: 5552 7004 7090 7102 7448 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1235 469 463 385 383 367
346 261 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3217 3576 4080 4224 4491 513
Size: 552 939 1213 359 504 144 267 3475
Fragments arranged by size:
3475 1213 939 552 504 359 267 144
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6714
Fragments arranged by size:
6714 683 56
BtsI GCAGTG_nn'
Cuts at: 2108 2141 2392 2822 2829 3302 3358 3907 4509
Size: 33 251 430 7 473 56 549 602
Cuts at: 4509 4969 5763 5783 2108
Size: 460 794 20 3778
Fragments arranged by size:
3778 794 602 549 473 460 430 251
56 33 20 7
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7453
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3291 3326 529
Size: 35 2727 35 4656
Fragments arranged by size:
4656 2727 35 35
DraI TTT'AAA
Cuts at: 4757 4757
Size: 7453
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2053 2606 2692 6460 809
Size: 507 737 553 86 3768 1802
Fragments arranged by size:
3768 1802 737 553 507 86
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3140 4077 4501
Size: 54 1425 50 275 18 1310 937 424
Cuts at: 4501 4900 5789 7418 8
Size: 399 889 1629 43
Fragments arranged by size:
1629 1425 1310 937 889 424 399 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4900 1537
Size: 275 3088 4090
Fragments arranged by size:
4090 3088 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3230 4220 5383 1353
Size: 1877 990 1163 3423
Fragments arranged by size:
3423 1877 1163 990
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 6040 6414 6560 387
Size: 1064 138 435 4016 374 146 1280
Fragments arranged by size:
4016 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 4213 4213
Size: 7453
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2444 3143 3415 3598 3802 3973 4384
Size: 393 812 699 272 183 204 171 411
Cuts at: 4384 5516 6113 6900 7168 1239
Size: 1132 597 787 268 1524
Fragments arranged by size:
1524 1132 812 787 699 597 411 393
272 268 204 183 171
EcoNI CCTnn'n_nnAGG
Cuts at: 2074 3419 2074
Size: 1345 6108
EcoO109I rG'GnC_Cy
Cuts at: 3804 3804
Size: 7453
EcoRI G'AATT_C
Cuts at: 4391 4762 5140 4391
Size: 371 378 6704
Fragments arranged by size:
6704 378 371
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3130 3899 3130
Size: 769 6684
FspI TGC'GCA
Cuts at: 5959 5959
Size: 7453
HaeII r_GCGC'y
Cuts at: 1678 3662 6600 1678
Size: 1984 2938 2531
Fragments arranged by size:
2938 2531 1984
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2726 2758 3611 3643 4112
Size: 32 135 32 1154 32 853 32 469
Cuts at: 4112 4144 4191 4223 4292 4324 6094 6126 6168
Size: 32 47 32 69 32 1770 32 42
Cuts at: 6168 6200 1373
Size: 32 2626
Fragments arranged by size:
2626 1770 1154 853 469 135 69 47
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3705 4797 5043 669
Size: 988 281 1767 1092 246 3079
Fragments arranged by size:
3079 1767 1092 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7453
HpaI GTT'AAC
Cuts at: 3705 5043 3705
Size: 1338 6115
KpnI G_GTAC'C
Cuts at: 4778 4778
Size: 7453
MfeI C'AATT_G
Cuts at: 5052 5052
Size: 7453
MluI A'CGCG_T
Cuts at: 7329 7329
Size: 7453
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2113 3435 6567 6751 1305
Size: 808 1322 3132 184 2007
Fragments arranged by size:
3132 2007 1322 808 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4079 4503 10
Size: 54 1425 343 2247 424 2960
Fragments arranged by size:
2960 2247 1425 424 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2453 3551 3575 4223 4469 5411 5770
Size: 648 1287 1098 24 648 246 942 359
Cuts at: 5770 5929 518
Size: 159 2042
Fragments arranged by size:
2042 1287 1098 942 648 648 359 246
159 24
MspA1I CmG'CkG
Cuts at: 1606 2163 3394 3828 4016 4037 4869 5546 6694
Size: 557 1231 434 188 21 832 677 1148
Cuts at: 6694 6939 1606
Size: 245 2120
Fragments arranged by size:
2120 1231 1148 832 677 557 434 245
188 21
NcoI C'CATG_G
Cuts at: 513 1065 2004 3217 4080 4224 4491 513
Size: 552 939 1213 863 144 267 3475
Fragments arranged by size:
3475 1213 939 863 552 267 144
NdeI CA'TA_TG
Cuts at: 387 4516 387
Size: 4129 3324
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7453
NotI GC'GGCC_GC
Cuts at: 4900 4900
Size: 7453
NspI r_CATG'y
Cuts at: 1132 1229 2173 2986 4151 4159 4417 4811 6356
Size: 97 944 813 1165 8 258 394 1545
Cuts at: 6356 1132
Size: 2229
Fragments arranged by size:
2229 1545 1165 944 813 394 258 97
8
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7453
PciI A'CATG_T
Cuts at: 2169 4413 6352 2169
Size: 2244 1939 3270
Fragments arranged by size:
3270 2244 1939
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4086 1108
Size: 2978 4475
PfoI T'CCnGG_A
Cuts at: 2610 3818 2610
Size: 1208 6245
PmeI GTTT'AAAC
Cuts at: 4757 4757
Size: 7453
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2412 2444 5522 5554 7035 7067 2412
Size: 32 3078 32 1481 32 2798
Fragments arranged by size:
3078 2798 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7453
PsiI TTA'TAA
Cuts at: 4647 5023 4647
Size: 376 7077
PstI C_TGCA'G
Cuts at: 830 3365 3454 3569 4805 830
Size: 2535 89 115 1236 3478
Fragments arranged by size:
3478 2535 1236 115 89
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7453
PvuII CAG'CTG
Cuts at: 2163 3828 4016 4037 2163
Size: 1665 188 21 5579
Fragments arranged by size:
5579 1665 188 21
SacI G_AGCT'C
Cuts at: 4215 4215
Size: 7453
SalI G'TCGA_C
Cuts at: 1936 4795 1936
Size: 2859 4594
SapI GCTCTTCn'nnn_
Cuts at: 4220 4220
Size: 7453
SbfI CC_TGCA'GG
Cuts at: 4805 4805
Size: 7453
ScaI AGT'ACT
Cuts at: 5701 7413 5701
Size: 1712 5741
SfcI C'TryA_G
Cuts at: 826 1045 3361 3450 3565 4801 5936 6617 6808
Size: 219 2316 89 115 1236 1135 681 191
Cuts at: 6808 826
Size: 1471
Fragments arranged by size:
2316 1471 1236 1135 681 219 191 115
89
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7453
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7453
SmaI CCC'GGG
Cuts at: 1872 4780 1872
Size: 2908 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2994 3710 5557 6458 6720 820
Size: 197 926 1051 716 1847 901 262 1553
Fragments arranged by size:
1847 1553 1051 926 901 716 262 197
SnaBI TAC'GTA
Cuts at: 493 5244 493
Size: 4751 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7453
SphI G_CATG'C
Cuts at: 1132 4811 1132
Size: 3679 3774
SspI AAT'ATT
Cuts at: 5 52 5377 5
Size: 47 5325 2081
Fragments arranged by size:
5325 2081 47
StuI AGG'CCT
Cuts at: 1517 4136 4447 1517
Size: 2619 311 4523
Fragments arranged by size:
4523 2619 311
StyI C'CwwG_G
Cuts at: 513 1065 2004 3217 3792 4080 4224 4234 4491
Size: 552 939 1213 575 288 144 10 257
Cuts at: 4491 4892 513
Size: 401 3074
Fragments arranged by size:
3074 1213 939 575 552 401 288 257
144 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2171 3956 5480 5497 5650 5835 6280
Size: 1255 591 1785 1524 17 153 185 445
Cuts at: 6280 325
Size: 1498
Fragments arranged by size:
1785 1524 1498 1255 591 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2172 5699 7411
Size: 274 80 33 51 1443 195 3527 1712
Cuts at: 7411 96
Size: 138
Fragments arranged by size:
3527 1712 1443 274 195 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3814 3846 7413 7445 3814
Size: 32 3567 32 3822
Fragments arranged by size:
3822 3567 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4382 1316
Size: 3066 4387
XbaI T'CTAG_A
Cuts at: 3067 4789 3067
Size: 1722 5731
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4324 4324
Size: 7453
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7453
XmaI C'CCGG_G
Cuts at: 1870 4778 1870
Size: 2908 4545
XmnI GAAnn'nnTTC
Cuts at: 2519 5582 2519
Size: 3063 4390
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5261 276
Size: 53 83 186 936 3727 2468
Fragments arranged by size:
3727 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbeI BbvCI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI