Restriction map for
pFN21AE3768
(Circular) (Six-base) MAPSORT of: pFN21AE3768.seq Check: 668 from: 1 to: 7567
pFN21AE3768 7567 bp
With 182 enzymes: SgfI *
March 28, 2012 12:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2416 2599 3330 2416
Size: 183 731 6653
Fragments arranged by size:
6653 731 183
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5377 278
Size: 53 83 186 936 3841 2468
Fragments arranged by size:
3841 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4910 1937
Size: 2973 4594
Acc65I G'GTAC_C
Cuts at: 4888 4888
Size: 7567
AclI AA'CG_TT
Cuts at: 1563 5694 6067 1563
Size: 4131 373 3063
Fragments arranged by size:
4131 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3752 5630 7014 7282 1632
Size: 2120 1878 1384 268 1917
Fragments arranged by size:
2120 1917 1878 1384 268
AfeI AGC'GCT
Cuts at: 2791 3242 2791
Size: 451 7116
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7370
AflIII A'CryG_T
Cuts at: 1203 3162 4277 6466 7443 1203
Size: 1959 1115 2189 977 1327
Fragments arranged by size:
2189 1959 1327 1115 977
AgeI A'CCGG_T
Cuts at: 6398 6398
Size: 7567
AhdI GACnn_n'nnGTC
Cuts at: 2674 6296 2674
Size: 3622 3945
AleI CACnn'nnGTG
Cuts at: 1166 4241 1166
Size: 3075 4492
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3410 3893 1184
Size: 2226 483 4858
Fragments arranged by size:
4858 2226 483
AlwNI CAG_nnn'CTG
Cuts at: 3280 4594 5310 6882 3280
Size: 1314 716 1572 3965
Fragments arranged by size:
3965 1572 1314 716
ApaI G_GGCC'C
Cuts at: 2061 2350 3633 3687 4211 4776 4855 2061
Size: 289 1283 54 524 565 79 4773
Fragments arranged by size:
4773 1283 565 524 289 79 54
ApaLI G'TGCA_C
Cuts at: 2544 5624 6780 2544
Size: 3080 1156 3331
Fragments arranged by size:
3331 3080 1156
ApoI r'AATT_y
Cuts at: 1493 4876 5102 5254 7363 1493
Size: 3383 226 152 2109 1697
Fragments arranged by size:
3383 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6121 160
Size: 872 5089 1606
Fragments arranged by size:
5089 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7567
AvaI C'yCGr_G
Cuts at: 1870 1943 2535 2616 2990 3354 3940 4523 4657
Size: 73 592 81 374 364 586 583 134
Cuts at: 4657 4892 1870
Size: 235 4545
Fragments arranged by size:
4545 592 586 583 374 364 235 134
81 73
BamHI G'GATC_C
Cuts at: 3494 4897 5264 3494
Size: 1403 367 5797
Fragments arranged by size:
5797 1403 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2240 2489 2575 2815 3952
Size: 325 217 627 453 249 86 240 1137
Cuts at: 3952 4001 4067 4225 4476 4888 6343 618
Size: 49 66 158 251 412 1455 1842
Fragments arranged by size:
1842 1455 1137 627 453 412 325 251
249 240 217 158 86 66 49
BanII G_rGCy'C
Cuts at: 1391 2061 2350 2458 2997 3633 3687 3936 4211
Size: 670 289 108 539 636 54 249 275
Cuts at: 4211 4530 4769 4776 4855 4886 1391
Size: 319 239 7 79 31 4072
Fragments arranged by size:
4072 670 636 539 319 289 275 249
239 108 79 54 31 7
BbeI G_GCGC'C
Cuts at: 2244 2493 2819 3956 4005 4071 4229 2244
Size: 249 326 1137 49 66 158 5582
Fragments arranged by size:
5582 1137 326 249 158 66 49
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2206 3144 4206 928
Size: 971 307 938 1062 4289
Fragments arranged by size:
4289 1062 971 938 307
BbvCI CC'TCA_GC
Cuts at: 3542 4166 3542
Size: 624 6943
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2981 3015 5758 5792 1903
Size: 34 1044 34 2743 34 3678
Fragments arranged by size:
3678 2743 1044 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5459 6669 5459
Size: 1210 6357
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7567
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2208 2813 4076
Size: 107 122 71 631 605 536 605 1263
Cuts at: 4076 4316 6178 136
Size: 240 1862 1525
Fragments arranged by size:
1862 1525 1263 631 605 605 536 240
122 107 71
BglII A'GATC_T
Cuts at: 7562 7562
Size: 7567
BlpI GC'TnA_GC
Cuts at: 3338 4984 3338
Size: 1646 5921
Bme1580I G_kGCm'C
Cuts at: 1790 2045 2061 2350 2548 2655 2990 3633 3687
Size: 255 16 289 198 107 335 643 54
Cuts at: 3687 3775 3882 4211 4479 4776 4842 4855 5628
Size: 88 107 329 268 297 66 13 773
Cuts at: 5628 6784 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 773 643 335 329 297 289
268 255 198 107 107 88 66 54
16 13
BmgBI CAC'GTC
Cuts at: 1318 4075 4204 4447 1318
Size: 2757 129 243 4438
Fragments arranged by size:
4438 2757 243 129
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2608 5298 6256
Size: 416 53 83 399 24 1191 2690 958
Cuts at: 6256 442
Size: 1753
Fragments arranged by size:
2690 1753 1191 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7567
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2249 2717 2884 3032 3134 3170 4415 4421
Size: 1010 468 167 148 102 36 1245 6
Cuts at: 4421 6227 1239
Size: 1806 2579
Fragments arranged by size:
2579 1806 1245 1010 468 167 148 102
36 6
Bpu10I CC'TnA_GC
Cuts at: 3542 4166 3542
Size: 624 6943
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2657 2720 3383 5692 6557 6855 2657
Size: 63 663 2309 865 298 3369
Fragments arranged by size:
3369 2309 865 663 298 63
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4654 4714 6230 882
Size: 617 3155 60 1516 2219
Fragments arranged by size:
3155 2219 1516 617 60
BsaAI yAC'GTr
Cuts at: 493 1204 2230 2587 2985 5358 493
Size: 711 1026 357 398 2373 2702
Fragments arranged by size:
2702 2373 1026 711 398 357
BsaBI GATnn'nnATC
Cuts at: 3299 7561 3299
Size: 4262 3305
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2211 2241 2490 2816
Size: 53 83 186 936 678 30 249 326
Cuts at: 2816 2925 3953 3968 4002 4068 4226 4487 5374
Size: 109 1028 15 34 66 158 261 887
Cuts at: 5374 5756 6391 275
Size: 382 635 1451
Fragments arranged by size:
1451 1028 936 887 678 635 382 326
261 249 186 158 109 83 66 53
34 30 15
BsaWI w'CCGG_w
Cuts at: 4759 6000 6398 6672 6819 7230 4759
Size: 1241 398 274 147 411 5096
Fragments arranged by size:
5096 1241 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2414 2444 3610 3640 4256
Size: 30 165 30 1011 30 1166 30 616
Cuts at: 4256 4286 1178
Size: 30 4459
Fragments arranged by size:
4459 1166 1011 616 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2027 2030 2117 2321 2378 3224 3227
Size: 838 3 3 87 204 57 846 3
Cuts at: 3227 3266 3732 3737 3857 4118 1186
Size: 39 466 5 120 261 4635
Fragments arranged by size:
4635 846 838 466 261 204 120 87
57 39 5 3 3 3
BseYI C'CCAG_C
Cuts at: 2195 2219 3021 3524 3686 3739 4048 4370 4504
Size: 24 802 503 162 53 309 322 134
Cuts at: 4504 4600 4742 4778 6770 7266 2195
Size: 96 142 36 1992 496 2496
Fragments arranged by size:
2496 1992 802 503 496 322 309 162
142 134 96 53 36 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2235 2627 3302 4805 1163
Size: 573 499 392 675 1503 3925
Fragments arranged by size:
3925 1503 675 573 499 392
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2128 2203 4095 4150 4192 4320
Size: 275 186 127 75 1892 55 42 128
Cuts at: 4320 4670 4720 5017 5778 6806 1540
Size: 350 50 297 761 1028 2301
Fragments arranged by size:
2301 1892 1028 761 350 297 275 186
128 127 75 55 50 42
BsiHKAI G_wGCw'C
Cuts at: 2548 2736 5628 5713 6784 2548
Size: 188 2892 85 1071 3331
Fragments arranged by size:
3331 2892 1071 188 85
BsmI GAATG_Cn'
Cuts at: 3023 5078 5171 3023
Size: 2055 93 5419
Fragments arranged by size:
5419 2055 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2837 2837
Size: 7567
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2061 2350 2458 2548 2655 2736
Size: 399 255 16 289 108 90 107 81
Cuts at: 2736 2990 2997 3633 3687 3775 3882 3936 4211
Size: 254 7 636 54 88 107 54 275
Cuts at: 4211 4479 4530 4769 4776 4842 4855 4886 5628
Size: 268 51 239 7 66 13 31 742
Cuts at: 5628 5713 6784 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 636 399 289 275 268
255 254 239 108 107 107 90 88
85 81 66 54 54 51 31 16
13 7 7
BspEI T'CCGG_A
Cuts at: 4759 7230 4759
Size: 2471 5096
BspHI T'CATG_A
Cuts at: 5456 7186 5456
Size: 1730 5837
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2416 2555 2599 3330 4922 844
Size: 1572 139 44 731 1592 3489
Fragments arranged by size:
3489 1592 1572 731 139 44
BsrBI CCG'CTC
Cuts at: 1394 1823 3709 3986 5454 1394
Size: 429 1886 277 1468 3507
Fragments arranged by size:
3507 1886 1468 429 277
BsrDI GCAATG_nn'
Cuts at: 65 1248 6062 6236 65
Size: 1183 4814 174 1396
Fragments arranged by size:
4814 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4145 4315 4479 4611 4669 6211 6398
Size: 110 2335 170 164 132 58 1542 187
Cuts at: 6398 1700
Size: 2869
Fragments arranged by size:
2869 2335 1542 187 170 164 132 110
58
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7567
BssHII G'CGCG_C
Cuts at: 1925 4085 4087 1925
Size: 2160 2 5405
Fragments arranged by size:
5405 2160 2
BssSI C'ACGA_G
Cuts at: 2291 4241 4551 5627 6639 2291
Size: 1950 310 1076 1012 3219
Fragments arranged by size:
3219 1950 1076 1012 310
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7567
BstBI TT'CG_AA
Cuts at: 6371 6371
Size: 7567
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7567
BstXI CCAn_nnnn'nTGG
Cuts at: 4048 4384 4544 4798 4048
Size: 336 160 254 6817
Fragments arranged by size:
6817 336 254 160
BstYI r'GATC_y
Cuts at: 1972 2280 3494 4897 5264 5649 5666 7118 7204
Size: 308 1214 1403 367 385 17 1452 86
Cuts at: 7204 7216 7562 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1403 1214 385 367 346 308
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2483 2483
Size: 7567
BtgI C'CryG_G
Cuts at: 513 1065 2004 2052 2070 2256 2934 3037 3564
Size: 552 939 48 18 186 678 103 527
Cuts at: 3564 3622 3956 4157 4186 4471 4530 4537 4855
Size: 58 334 201 29 285 59 7 318
Cuts at: 4855 513
Size: 3225
Fragments arranged by size:
3225 939 678 552 527 334 318 285
201 186 103 59 58 48 29 18
7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3793 487
Size: 683 56 2567 4261
Fragments arranged by size:
4261 2567 683 56
BtsI GCAGTG_nn'
Cuts at: 2418 2860 3592 3604 3920 4283 4793 5083 5877
Size: 442 732 12 316 363 510 290 794
Cuts at: 5877 5897 2418
Size: 20 4088
Fragments arranged by size:
4088 794 732 510 442 363 316 290
20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7567
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7532
DraI TTT'AAA
Cuts at: 4871 4871
Size: 7567
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6574 809
Size: 507 5258 1802
Fragments arranged by size:
5258 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2200 2931
Size: 54 1425 50 275 18 198 172 731
Cuts at: 2931 3044 4077 4092 4147 4189 4233 4317 4667
Size: 113 1033 15 55 42 44 84 350
Cuts at: 4667 4717 5014 5903 7532 8
Size: 50 297 889 1629 43
Fragments arranged by size:
1629 1425 1033 889 731 350 297 275
198 172 113 84 55 54 50 50
44 43 42 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2200 4092 4147 4189 4317 4667 4717
Size: 275 388 1892 55 42 128 350 50
Cuts at: 4717 5014 1537
Size: 297 4090
Fragments arranged by size:
4090 1892 388 350 297 275 128 55
50 42
EarI CTCTTCn'nnn_
Cuts at: 1353 2846 4136 5497 1353
Size: 1493 1290 1361 3423
Fragments arranged by size:
3423 1493 1361 1290
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4498 6154 6528 6674 387
Size: 1064 138 2909 1656 374 146 1280
Fragments arranged by size:
2909 1656 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2249 2717 2884 3032 3134 3170 3752
Size: 393 617 468 167 148 102 36 582
Cuts at: 3752 4415 4421 5630 6227 7014 7282 1239
Size: 663 6 1209 597 787 268 1524
Fragments arranged by size:
1524 1209 787 663 617 597 582 468
393 268 167 148 102 36 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3011 3701 3011
Size: 690 6877
EcoO109I rG'GnC_Cy
Cuts at: 2057 2347 2404 2668 2799 2905 3025 3630 3683
Size: 290 57 264 131 106 120 605 53
Cuts at: 3683 4162 4208 4417 4591 4773 4851 2057
Size: 479 46 209 174 182 78 4773
Fragments arranged by size:
4773 605 479 290 264 209 182 174
131 120 106 78 57 53 46
EcoRI G'AATT_C
Cuts at: 4876 5254 4876
Size: 378 7189
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3394 3394
Size: 7567
FspI TGC'GCA
Cuts at: 6073 6073
Size: 7567
HaeII r_GCGC'y
Cuts at: 1678 2244 2493 2793 2819 3244 3956 4005 4015
Size: 566 249 300 26 425 712 49 10
Cuts at: 4015 4028 4071 4117 4229 4505 6714 1678
Size: 13 43 46 112 276 2209 2531
Fragments arranged by size:
2531 2209 712 566 425 300 276 249
112 49 46 43 26 13 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2110 2142 2660 2692 4256
Size: 32 135 32 538 32 518 32 1564
Cuts at: 4256 4288 6208 6240 6282 6314 1373
Size: 32 1920 32 42 32 2626
Fragments arranged by size:
2626 1920 1564 538 518 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2596 4911 5157 669
Size: 988 281 658 2315 246 3079
Fragments arranged by size:
3079 2315 988 658 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7567
HpaI GTT'AAC
Cuts at: 5157 5157
Size: 7567
KasI G'GCGC_C
Cuts at: 2240 2489 2815 3952 4001 4067 4225 2240
Size: 249 326 1137 49 66 158 5582
Fragments arranged by size:
5582 1137 326 249 158 66 49
KpnI G_GTAC'C
Cuts at: 4892 4892
Size: 7567
MfeI C'AATT_G
Cuts at: 5166 5166
Size: 7567
MluI A'CGCG_T
Cuts at: 4277 7443 4277
Size: 3166 4401
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3497 4287 6681 6865 1305
Size: 2192 790 2394 184 2007
Fragments arranged by size:
2394 2192 2007 790 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2030 10
Size: 54 1425 343 198 5547
Fragments arranged by size:
5547 1425 343 198 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2318 2640 2783 4241 4808 5525 5884
Size: 648 1152 322 143 1458 567 717 359
Cuts at: 5884 6043 518
Size: 159 2042
Fragments arranged by size:
2042 1458 1152 717 648 567 359 322
159 143
MspA1I CmG'CkG
Cuts at: 1606 2054 2072 2199 2249 2258 2269 2420 3259
Size: 448 18 127 50 9 11 151 839
Cuts at: 3259 3337 3624 4111 4159 4473 4513 4532 4983
Size: 78 287 487 48 314 40 19 451
Cuts at: 4983 5660 6808 7053 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 839 677 487 451 448 314
287 245 151 127 78 50 48 40
19 18 11 9
NaeI GCC'GGC
Cuts at: 4147 4613 4671 4147
Size: 466 58 7043
Fragments arranged by size:
7043 466 58
NarI GG'CG_CC
Cuts at: 2241 2490 2816 3953 4002 4068 4226 2241
Size: 249 326 1137 49 66 158 5582
Fragments arranged by size:
5582 1137 326 249 158 66 49
NcoI C'CATG_G
Cuts at: 513 1065 2004 4537 513
Size: 552 939 2533 3543
Fragments arranged by size:
3543 2533 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7567
NgoMIV G'CCGG_C
Cuts at: 4145 4611 4669 4145
Size: 466 58 7043
Fragments arranged by size:
7043 466 58
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7567
NotI GC'GGCC_GC
Cuts at: 2200 5014 2200
Size: 2814 4753
NspI r_CATG'y
Cuts at: 1132 1229 3166 4461 4925 6470 1132
Size: 97 1937 1295 464 1545 2229
Fragments arranged by size:
2229 1937 1545 1295 464 97
PasI CC'CwG_GG
Cuts at: 1348 4769 1348
Size: 3421 4146
PciI A'CATG_T
Cuts at: 3162 6466 3162
Size: 3304 4263
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2037 4496 1108
Size: 929 2459 4179
Fragments arranged by size:
4179 2459 929
PfoI T'CCnGG_A
Cuts at: 2694 3009 3246 2694
Size: 315 237 7015
Fragments arranged by size:
7015 315 237
PmeI GTTT'AAAC
Cuts at: 4871 4871
Size: 7567
PmlI CAC'GTG
Cuts at: 2587 2985 2587
Size: 398 7169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5636 5668 7149 7181 5636
Size: 32 1481 32 6022
Fragments arranged by size:
6022 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2404 2668 2799 2905 3025 4417 2404
Size: 264 131 106 120 1392 5554
Fragments arranged by size:
5554 1392 264 131 120 106
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7567
PsiI TTA'TAA
Cuts at: 5137 5137
Size: 7567
PspOMI G'GGCC_C
Cuts at: 2057 2346 3629 3683 4207 4772 4851 2057
Size: 289 1283 54 524 565 79 4773
Fragments arranged by size:
4773 1283 565 524 289 79 54
PstI C_TGCA'G
Cuts at: 830 2301 2463 3135 3702 4919 830
Size: 1471 162 672 567 1217 3478
Fragments arranged by size:
3478 1471 1217 672 567 162
PvuI CG_AT'CG
Cuts at: 2001 2128 2001
Size: 127 7440
PvuII CAG'CTG
Cuts at: 2269 3259 3337 2269
Size: 990 78 6499
Fragments arranged by size:
6499 990 78
SacII CC_GC'GG
Cuts at: 2055 2073 2259 3625 4160 4474 4533 2055
Size: 18 186 1366 535 314 59 5089
Fragments arranged by size:
5089 1366 535 314 186 59 18
SalI G'TCGA_C
Cuts at: 1936 4909 1936
Size: 2973 4594
SanDI GG'GwC_CC
Cuts at: 3025 3025
Size: 7567
SbfI CC_TGCA'GG
Cuts at: 4919 4919
Size: 7567
ScaI AGT'ACT
Cuts at: 5815 7527 5815
Size: 1712 5855
SfcI C'TryA_G
Cuts at: 826 1045 2297 2459 3131 3698 4915 6050 6731
Size: 219 1252 162 672 567 1217 1135 681
Cuts at: 6731 6922 826
Size: 191 1471
Fragments arranged by size:
1471 1252 1217 1135 681 672 567 219
191 162
SfiI GGCCn_nnn'nGGCC
Cuts at: 4316 4316
Size: 7567
SfoI GGC'GCC
Cuts at: 2242 2491 2817 3954 4003 4069 4227 2242
Size: 249 326 1137 49 66 158 5582
Fragments arranged by size:
5582 1137 326 249 158 66 49
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7567
SgrAI Cr'CCGG_yG
Cuts at: 1700 4479 1700
Size: 2779 4788
SmaI CCC'GGG
Cuts at: 1872 2537 2618 3356 3942 4894 1872
Size: 665 81 738 586 952 4545
Fragments arranged by size:
4545 952 738 665 586 81
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2672 2735 3398 5671 6572 6834
Size: 197 926 729 63 663 2273 901 262
Cuts at: 6834 820
Size: 1553
Fragments arranged by size:
2273 1553 926 901 729 663 262 197
63
SnaBI TAC'GTA
Cuts at: 493 5358 493
Size: 4865 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7567
SphI G_CATG'C
Cuts at: 1132 4461 4925 1132
Size: 3329 464 3774
Fragments arranged by size:
3774 3329 464
SspI AAT'ATT
Cuts at: 5 52 5491 5
Size: 47 5439 2081
Fragments arranged by size:
5439 2081 47
StuI AGG'CCT
Cuts at: 1517 3467 1517
Size: 1950 5617
StyI C'CwwG_G
Cuts at: 513 1065 2004 2031 2928 3183 4537 5006 513
Size: 552 939 27 897 255 1354 469 3074
Fragments arranged by size:
3074 1354 939 897 552 469 255 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3820 4372 4457 5594 5611 5764 5949
Size: 1255 2240 552 85 1137 17 153 185
Cuts at: 5949 6394 325
Size: 445 1498
Fragments arranged by size:
2240 1498 1255 1137 552 445 185 153
85 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5813 7525 96
Size: 274 80 33 51 1443 3836 1712 138
Fragments arranged by size:
3836 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2414 2446 3610 3642 7527 7559 2414
Size: 32 1164 32 3885 32 2422
Fragments arranged by size:
3885 2422 1164 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3901 3970 1316
Size: 2585 69 4913
Fragments arranged by size:
4913 2585 69
XbaI T'CTAG_A
Cuts at: 4903 4903
Size: 7567
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2203 2227 4384 2203
Size: 24 2157 5386
Fragments arranged by size:
5386 2157 24
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7567
XmaI C'CCGG_G
Cuts at: 1870 2535 2616 3354 3940 4892 1870
Size: 665 81 738 586 952 4545
Fragments arranged by size:
4545 952 738 665 586 81
XmnI GAAnn'nnTTC
Cuts at: 5696 5696
Size: 7567
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5375 276
Size: 53 83 186 936 3841 2468
Fragments arranged by size:
3841 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I
DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI
PspXI PsrI RsrII SacI SapI SexAI SrfI SwaI