Restriction map for
pFN21AE3767
(Circular) (Six-base) MAPSORT of: pFN21AE3767.seq Check: 3025 from: 1 to: 6970
pFN21AE3767 6970 bp
With 182 enzymes: SgfI *
March 28, 2012 12:05 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4143 4780 278
Size: 53 83 186 936 2607 637 2468
Fragments arranged by size:
2607 2468 936 637 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4313 1937
Size: 2376 4594
Acc65I G'GTAC_C
Cuts at: 4249 4291 4249
Size: 42 6928
AclI AA'CG_TT
Cuts at: 1563 5097 5470 1563
Size: 3534 373 3063
Fragments arranged by size:
3534 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2328 2462 2778 3486 3746 4008 5033 6417
Size: 696 134 316 708 260 262 1025 1384
Cuts at: 6417 6685 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1025 708 696 316 268 262
260 134
AflII C'TTAA_G
Cuts at: 820 1017 3925 820
Size: 197 2908 3865
Fragments arranged by size:
3865 2908 197
AflIII A'CryG_T
Cuts at: 1203 3015 5869 6846 1203
Size: 1812 2854 977 1327
Fragments arranged by size:
2854 1812 1327 977
AgeI A'CCGG_T
Cuts at: 3846 4197 5801 3846
Size: 351 1604 5015
Fragments arranged by size:
5015 1604 351
AhdI GACnn_n'nnGTC
Cuts at: 5699 5699
Size: 6970
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6970
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6970
AlwNI CAG_nnn'CTG
Cuts at: 3464 3724 3826 4234 4713 6285 3464
Size: 260 102 408 479 1572 4149
Fragments arranged by size:
4149 1572 479 408 260 102
ApaI G_GGCC'C
Cuts at: 2216 2216
Size: 6970
ApaLI G'TGCA_C
Cuts at: 2060 5027 6183 2060
Size: 2967 1156 2847
Fragments arranged by size:
2967 2847 1156
ApoI r'AATT_y
Cuts at: 1493 2275 2642 2738 4279 4505 4657 6766 1493
Size: 782 367 96 1541 226 152 2109 1697
Fragments arranged by size:
2109 1697 1541 782 367 226 152 96
AseI AT'TA_AT
Cuts at: 160 1032 5524 160
Size: 872 4492 1606
Fragments arranged by size:
4492 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6970
AvaI C'yCGr_G
Cuts at: 1870 1943 4295 1870
Size: 73 2352 4545
Fragments arranged by size:
4545 2352 73
BamHI G'GATC_C
Cuts at: 4300 4667 4300
Size: 367 6603
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2153 4249 4291 5746 618
Size: 325 217 627 366 2096 42 1455 1842
Fragments arranged by size:
2096 1842 1455 627 366 325 217 42
BanII G_rGCy'C
Cuts at: 1391 2216 2970 3255 3954 4289 1391
Size: 825 754 285 699 335 4072
Fragments arranged by size:
4072 825 754 699 335 285
BbeI G_GCGC'C
Cuts at: 2157 2157
Size: 6970
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3188 3923 4187 928
Size: 971 1289 735 264 3711
Fragments arranged by size:
3711 1289 971 735 264
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5161 5195 1903
Size: 34 3224 34 3678
Fragments arranged by size:
3678 3224 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4862 6072 4862
Size: 1210 5760
BclI T'GATC_A
Cuts at: 1548 3535 3744 1548
Size: 1987 209 4774
Fragments arranged by size:
4774 1987 209
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2145 5581 136
Size: 107 122 71 631 605 473 3436 1525
Fragments arranged by size:
3436 1525 631 605 473 122 107 71
BglII A'GATC_T
Cuts at: 6965 6965
Size: 6970
BlpI GC'TnA_GC
Cuts at: 3341 4387 3341
Size: 1046 5924
Bme1580I G_kGCm'C
Cuts at: 1790 2064 2216 5031 6187 1790
Size: 274 152 2815 1156 2573
Fragments arranged by size:
2815 2573 1156 274 152
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6970
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2098 3511 4701
Size: 416 53 83 399 24 681 1413 1190
Cuts at: 4701 5659 442
Size: 958 1753
Fragments arranged by size:
1753 1413 1190 958 681 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6970
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4255 4255
Size: 6970
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2685 3008 3107 3310 3377 4085 5630 1239
Size: 1446 323 99 203 67 708 1545 2579
Fragments arranged by size:
2579 1545 1446 708 323 203 99 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2923 5095 5960 6258 2923
Size: 2172 865 298 3635
Fragments arranged by size:
3635 2172 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5633 882
Size: 617 4134 2219
Fragments arranged by size:
4134 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3016 4761 493
Size: 711 1812 1745 2702
Fragments arranged by size:
2702 1812 1745 711
BsaBI GATnn'nnATC
Cuts at: 2291 6964 2291
Size: 4673 2297
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2096 2154 4140 4777
Size: 53 83 186 936 563 58 1986 637
Cuts at: 4777 5159 5794 275
Size: 382 635 1451
Fragments arranged by size:
1986 1451 936 637 635 563 382 186
83 58 53
BsaWI w'CCGG_w
Cuts at: 2855 3846 4197 5403 5801 6075 6222 6633 2855
Size: 991 351 1206 398 274 147 411 3192
Fragments arranged by size:
3192 1206 991 411 398 351 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2808 2838 3119 3149 3833
Size: 30 165 30 1405 30 281 30 684
Cuts at: 3833 3863 1178
Size: 30 4285
Fragments arranged by size:
4285 1405 684 281 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2615 2816 1186
Size: 1429 201 5340
Fragments arranged by size:
5340 1429 201
BseYI C'CCAG_C
Cuts at: 3052 3176 6173 6669 3052
Size: 124 2997 496 3353
Fragments arranged by size:
3353 2997 496 124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2303 2918 3212 3398 1163
Size: 573 567 615 294 186 4735
Fragments arranged by size:
4735 615 573 567 294 186
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4420 5181 6209 1540
Size: 275 186 2419 761 1028 2301
Fragments arranged by size:
2419 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2064 2970 3255 5031 5116 6187 2064
Size: 906 285 1776 85 1071 2847
Fragments arranged by size:
2847 1776 1071 906 285 85
BsmI GAATG_Cn'
Cuts at: 3091 3373 4481 4574 3091
Size: 282 1108 93 5487
Fragments arranged by size:
5487 1108 282 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2216 2970 3255 3954 4289 5031
Size: 399 274 152 754 285 699 335 742
Cuts at: 5031 5116 6187 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 754 742 699 399 335 285
274 152 85
BspEI T'CCGG_A
Cuts at: 6633 6633
Size: 6970
BspHI T'CATG_A
Cuts at: 4859 6589 4859
Size: 1730 5240
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4325 844
Size: 3481 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2173 2915 4857 1394
Size: 429 350 742 1942 3507
Fragments arranged by size:
3507 1942 742 429 350
BsrDI GCAATG_nn'
Cuts at: 65 1248 3567 5465 5639 65
Size: 1183 2319 1898 174 1396
Fragments arranged by size:
2319 1898 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3846 4197 5614 5801 1700
Size: 110 2036 351 1417 187 2869
Fragments arranged by size:
2869 2036 1417 351 187 110
BsrGI T'GTAC_A
Cuts at: 96 2466 96
Size: 2370 4600
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6970
BssSI C'ACGA_G
Cuts at: 2018 5030 6042 2018
Size: 3012 1012 2946
Fragments arranged by size:
3012 2946 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6970
BstBI TT'CG_AA
Cuts at: 5774 5774
Size: 6970
BstEII G'GTnAC_C
Cuts at: 1184 3120 1184
Size: 1936 5034
BstYI r'GATC_y
Cuts at: 1972 3065 4300 4667 5052 5069 6521 6607 6619
Size: 1093 1235 367 385 17 1452 86 12
Cuts at: 6619 6965 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1235 1093 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3312 3762 513
Size: 552 939 1308 450 3721
Fragments arranged by size:
3721 1308 939 552 450
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3253 3784 4220 487
Size: 683 56 2027 531 436 3237
Fragments arranged by size:
3237 2027 683 531 436 56
BtsI GCAGTG_nn'
Cuts at: 2469 2620 2935 3317 4486 5280 5300 2469
Size: 151 315 382 1169 794 20 4139
Fragments arranged by size:
4139 1169 794 382 315 151 20
ClaI AT'CG_AT
Cuts at: 1554 3258 1554
Size: 1704 5266
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6935
DraI TTT'AAA
Cuts at: 4274 4274
Size: 6970
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5977 809
Size: 507 4661 1802
Fragments arranged by size:
4661 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2893 3282
Size: 54 1425 50 275 18 177 886 389
Cuts at: 3282 3301 3942 4005 4417 5306 6935 8
Size: 19 641 63 412 889 1629 43
Fragments arranged by size:
1629 1425 889 886 641 412 389 275
177 63 54 50 43 19 18
EagI C'GGCC_G
Cuts at: 1537 1812 4417 1537
Size: 275 2605 4090
Fragments arranged by size:
4090 2605 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2247 2592 2877 3056 4217 4900 1353
Size: 894 345 285 179 1161 683 3423
Fragments arranged by size:
3423 1161 894 683 345 285 179
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2191 3486 5557 5931 6077 387
Size: 1064 138 602 1295 2071 374 146 1280
Fragments arranged by size:
2071 1295 1280 1064 602 374 146 138
EcoICRI GAG'CTC
Cuts at: 2968 3253 2968
Size: 285 6685
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2328 2462 2685 2778 3008 3107 3310
Size: 393 696 134 223 93 230 99 203
Cuts at: 3310 3377 3486 3746 4008 4085 5033 5630 6417
Size: 67 109 260 262 77 948 597 787
Cuts at: 6417 6685 1239
Size: 268 1524
Fragments arranged by size:
1524 948 787 696 597 393 268 262
260 230 223 203 134 109 99 93
77 67
EcoNI CCTnn'n_nnAGG
Cuts at: 2207 2978 3836 2207
Size: 771 858 5341
Fragments arranged by size:
5341 858 771
EcoO109I rG'GnC_Cy
Cuts at: 2212 2692 2212
Size: 480 6490
EcoRI G'AATT_C
Cuts at: 4279 4657 4279
Size: 378 6592
EcoRV GAT'ATC
Cuts at: 3262 3262
Size: 6970
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2594 2594
Size: 6970
FspI TGC'GCA
Cuts at: 3894 5476 3894
Size: 1582 5388
HaeII r_GCGC'y
Cuts at: 1678 2157 6117 1678
Size: 479 3960 2531
Fragments arranged by size:
3960 2531 479
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2345 2377 2591 2623 4249
Size: 32 135 32 773 32 214 32 1626
Cuts at: 4249 4281 5611 5643 5685 5717 1373
Size: 32 1330 32 42 32 2626
Fragments arranged by size:
2626 1626 1330 773 214 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4117 4314 4560 669
Size: 988 281 2179 197 246 3079
Fragments arranged by size:
3079 2179 988 281 246 197
HindIII A'AGCT_T
Cuts at: 748 2438 3689 748
Size: 1690 1251 4029
Fragments arranged by size:
4029 1690 1251
HpaI GTT'AAC
Cuts at: 4560 4560
Size: 6970
KasI G'GCGC_C
Cuts at: 2153 2153
Size: 6970
KpnI G_GTAC'C
Cuts at: 4253 4295 4253
Size: 42 6928
MfeI C'AATT_G
Cuts at: 4569 4569
Size: 6970
MluI A'CGCG_T
Cuts at: 6846 6846
Size: 6970
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3030 3243 6084 6268 1305
Size: 1725 213 2841 184 2007
Fragments arranged by size:
2841 2007 1725 213 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 3944 4007 10
Size: 54 1425 343 177 1935 63 2973
Fragments arranged by size:
2973 1935 1425 343 177 63 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2067 3737 4928 5287 5446 518
Size: 648 901 1670 1191 359 159 2042
Fragments arranged by size:
2042 1670 1191 901 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2191 2269 4222 4386 5063 6211 6456 1606
Size: 585 78 1953 164 677 1148 245 2120
Fragments arranged by size:
2120 1953 1148 677 585 245 164 78
NarI GG'CG_CC
Cuts at: 2154 2154
Size: 6970
NcoI C'CATG_G
Cuts at: 513 1065 2004 3312 513
Size: 552 939 1308 4171
Fragments arranged by size:
4171 1308 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6970
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6970
NotI GC'GGCC_GC
Cuts at: 4417 4417
Size: 6970
NspI r_CATG'y
Cuts at: 1132 1229 4328 5873 1132
Size: 97 3099 1545 2229
Fragments arranged by size:
3099 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3139 4174 1348
Size: 1791 1035 4144
Fragments arranged by size:
4144 1791 1035
PciI A'CATG_T
Cuts at: 5869 5869
Size: 6970
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4174 1108
Size: 3066 3904
PfoI T'CCnGG_A
Cuts at: 3975 3975
Size: 6970
PmeI GTTT'AAAC
Cuts at: 4274 4274
Size: 6970
PmlI CAC'GTG
Cuts at: 3016 3016
Size: 6970
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5039 5071 6552 6584 5039
Size: 32 1481 32 5425
Fragments arranged by size:
5425 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3984 1613
Size: 2371 4599
PsiI TTA'TAA
Cuts at: 4540 4540
Size: 6970
PspOMI G'GGCC_C
Cuts at: 2212 2212
Size: 6970
PstI C_TGCA'G
Cuts at: 830 2211 2979 3460 3822 4322 830
Size: 1381 768 481 362 500 3478
Fragments arranged by size:
3478 1381 768 500 481 362
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6970
PvuII CAG'CTG
Cuts at: 2191 2269 4222 2191
Size: 78 1953 4939
Fragments arranged by size:
4939 1953 78
SacI G_AGCT'C
Cuts at: 2970 3255 2970
Size: 285 6685
SalI G'TCGA_C
Cuts at: 1936 4312 1936
Size: 2376 4594
SapI GCTCTTCn'nnn_
Cuts at: 4217 4217
Size: 6970
SbfI CC_TGCA'GG
Cuts at: 2979 3822 4322 2979
Size: 843 500 5627
Fragments arranged by size:
5627 843 500
ScaI AGT'ACT
Cuts at: 2486 3857 5218 6930 2486
Size: 1371 1361 1712 2526
Fragments arranged by size:
2526 1712 1371 1361
SfcI C'TryA_G
Cuts at: 826 1045 2207 2431 2975 3456 3818 4318 5453
Size: 219 1162 224 544 481 362 500 1135
Cuts at: 5453 6134 6325 826
Size: 681 191 1471
Fragments arranged by size:
1471 1162 1135 681 544 500 481 362
224 219 191
SfoI GGC'GCC
Cuts at: 2155 2155
Size: 6970
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6970
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6970
SmaI CCC'GGG
Cuts at: 1872 4297 1872
Size: 2425 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2902 3925 5074 5975 6237 820
Size: 197 926 959 1023 1149 901 262 1553
Fragments arranged by size:
1553 1149 1023 959 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4761 493
Size: 4268 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6970
SphI G_CATG'C
Cuts at: 1132 4328 1132
Size: 3196 3774
SspI AAT'ATT
Cuts at: 5 52 4894 5
Size: 47 4842 2081
Fragments arranged by size:
4842 2081 47
StuI AGG'CCT
Cuts at: 1517 3923 1517
Size: 2406 4564
StyI C'CwwG_G
Cuts at: 513 1065 2004 3312 3837 4409 513
Size: 552 939 1308 525 572 3074
Fragments arranged by size:
3074 1308 939 572 552 525
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2080 2936 4997 5014 5167 5352 5797
Size: 1255 500 856 2061 17 153 185 445
Cuts at: 5797 325
Size: 1498
Fragments arranged by size:
2061 1498 1255 856 500 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2466 2484 3109
Size: 274 80 33 51 1443 489 18 625
Cuts at: 3109 3774 3855 5216 6928 96
Size: 665 81 1361 1712 138
Fragments arranged by size:
1712 1443 1361 665 625 489 274 138
81 80 51 33 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2808 2840 3517 3549 4186 4218 6930 6962 2808
Size: 32 677 32 637 32 2712 32 2816
Fragments arranged by size:
2816 2712 677 637 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6970
XbaI T'CTAG_A
Cuts at: 4306 4306
Size: 6970
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4000 4015 4000
Size: 15 6955
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6970
XmaI C'CCGG_G
Cuts at: 1870 4295 1870
Size: 2425 4545
XmnI GAAnn'nnTTC
Cuts at: 2646 3492 5099 2646
Size: 846 1607 4517
Fragments arranged by size:
4517 1607 846
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4141 4778 276
Size: 53 83 186 936 2607 637 2468
Fragments arranged by size:
2607 2468 936 637 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII FseI
FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI