Restriction map for
pFN21AE3761
(Circular) (Six-base) MAPSORT of: pFN21AE3761.seq Check: 3696 from: 1 to: 6361
pFN21AE3761 6361 bp
With 182 enzymes: SgfI *
March 28, 2012 12:05 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4171 278
Size: 53 83 186 936 2635 2468
Fragments arranged by size:
2635 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3704 1937
Size: 1767 4594
Acc65I G'GTAC_C
Cuts at: 3682 3682
Size: 6361
AclI AA'CG_TT
Cuts at: 1563 4488 4861 1563
Size: 2925 373 3063
Fragments arranged by size:
3063 2925 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2571 2604 2859 3276 3567 4424 5808 6076
Size: 939 33 255 417 291 857 1384 268
Cuts at: 6076 1632
Size: 1917
Fragments arranged by size:
1917 1384 939 857 417 291 268 255
33
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6164
AflIII A'CryG_T
Cuts at: 1203 5260 6237 1203
Size: 4057 977 1327
Fragments arranged by size:
4057 1327 977
AgeI A'CCGG_T
Cuts at: 5192 5192
Size: 6361
AhdI GACnn_n'nnGTC
Cuts at: 5090 5090
Size: 6361
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6361
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6361
AlwNI CAG_nnn'CTG
Cuts at: 2374 2549 2582 3344 4104 5676 2374
Size: 175 33 762 760 1572 3059
Fragments arranged by size:
3059 1572 762 760 175 33
ApaLI G'TGCA_C
Cuts at: 4418 5574 4418
Size: 1156 5205
ApoI r'AATT_y
Cuts at: 1493 2196 2507 2900 3670 3896 4048 6157 1493
Size: 703 311 393 770 226 152 2109 1697
Fragments arranged by size:
2109 1697 770 703 393 311 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4915 160
Size: 872 3883 1606
Fragments arranged by size:
3883 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6361
AvaI C'yCGr_G
Cuts at: 1870 1943 3686 1870
Size: 73 1743 4545
Fragments arranged by size:
4545 1743 73
BamHI G'GATC_C
Cuts at: 3691 4058 3691
Size: 367 5994
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2078 2633 3682 5137 618
Size: 325 217 627 291 555 1049 1455 1842
Fragments arranged by size:
1842 1455 1049 627 555 325 291 217
BanII G_rGCy'C
Cuts at: 1391 3680 1391
Size: 2289 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2847 3573 928
Size: 971 948 726 3716
Fragments arranged by size:
3716 971 948 726
BbvCI CC'TCA_GC
Cuts at: 3462 3462
Size: 6361
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4552 4586 1903
Size: 34 2615 34 3678
Fragments arranged by size:
3678 2615 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4253 5463 4253
Size: 1210 5151
BclI T'GATC_A
Cuts at: 1548 2250 1548
Size: 702 5659
BfrBI ATG'CAT
Cuts at: 2743 3076 3308 2743
Size: 333 232 5796
Fragments arranged by size:
5796 333 232
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4972 136
Size: 107 122 71 631 605 3300 1525
Fragments arranged by size:
3300 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3443 6356 3443
Size: 2913 3448
BlpI GC'TnA_GC
Cuts at: 3778 3778
Size: 6361
Bme1580I G_kGCm'C
Cuts at: 1790 4422 5578 1790
Size: 2632 1156 2573
Fragments arranged by size:
2632 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6361
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2817 4092 5050
Size: 416 53 83 399 24 1400 1275 958
Cuts at: 5050 442
Size: 1753
Fragments arranged by size:
1753 1400 1275 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6361
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2316 2617 5021 1239
Size: 1077 301 2404 2579
Fragments arranged by size:
2579 2404 1077 301
Bpu10I CC'TnA_GC
Cuts at: 3462 3462
Size: 6361
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2937 4486 5351 5649 2937
Size: 1549 865 298 3649
Fragments arranged by size:
3649 1549 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2412 5024 882
Size: 617 913 2612 2219
Fragments arranged by size:
2612 2219 913 617
BsaAI yAC'GTr
Cuts at: 493 1204 2053 3355 4152 493
Size: 711 849 1302 797 2702
Fragments arranged by size:
2702 1302 849 797 711
BsaBI GATnn'nnATC
Cuts at: 6355 6355
Size: 6361
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2073 4168 4550 5185
Size: 53 83 186 936 540 2095 382 635
Cuts at: 5185 275
Size: 1451
Fragments arranged by size:
2095 1451 936 635 540 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3566 4794 5192 5466 5613 6024 3566
Size: 1228 398 274 147 411 3903
Fragments arranged by size:
3903 1228 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2590 2620 1178
Size: 30 165 30 1187 30 4919
Fragments arranged by size:
4919 1187 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6361
BseYI C'CCAG_C
Cuts at: 2056 2068 5564 6060 2056
Size: 12 3496 496 2357
Fragments arranged by size:
3496 2357 496 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5788
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3811 4572 5600 1540
Size: 275 186 1810 761 1028 2301
Fragments arranged by size:
2301 1810 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4422 4507 5578 4422
Size: 85 1071 5205
Fragments arranged by size:
5205 1071 85
BsmI GAATG_Cn'
Cuts at: 2464 3310 3872 3965 2464
Size: 846 562 93 4860
Fragments arranged by size:
4860 846 562 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3680 4422 4507 5578 1391
Size: 399 1890 742 85 1071 2174
Fragments arranged by size:
2174 1890 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6024 6024
Size: 6361
BspHI T'CATG_A
Cuts at: 4250 5980 4250
Size: 1730 4631
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2567 2743 3716 844
Size: 1723 176 973 3489
Fragments arranged by size:
3489 1723 973 176
BsrBI CCG'CTC
Cuts at: 1394 1823 4248 1394
Size: 429 2425 3507
Fragments arranged by size:
3507 2425 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4856 5030 65
Size: 1183 3608 174 1396
Fragments arranged by size:
3608 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5005 5192 1700
Size: 110 3195 187 2869
Fragments arranged by size:
3195 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2909 96
Size: 2813 3548
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6361
BssSI C'ACGA_G
Cuts at: 4421 5433 4421
Size: 1012 5349
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2749 794
Size: 1955 4406
BstBI TT'CG_AA
Cuts at: 5165 5165
Size: 6361
BstEII G'GTnAC_C
Cuts at: 1184 2945 1184
Size: 1761 4600
BstYI r'GATC_y
Cuts at: 1972 2528 2679 3443 3691 4058 4443 4460 5912
Size: 556 151 764 248 367 385 17 1452
Cuts at: 5912 5998 6010 6356 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 764 556 385 367 346 248
151 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3612 513
Size: 552 939 1608 3262
Fragments arranged by size:
3262 1608 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5622
Fragments arranged by size:
5622 683 56
BtsI GCAGTG_nn'
Cuts at: 2973 3877 4671 4691 2973
Size: 904 794 20 4643
Fragments arranged by size:
4643 904 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6361
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6326
DraI TTT'AAA
Cuts at: 3412 3665 3412
Size: 253 6108
DraIII CAC_nnn'GTG
Cuts at: 2053 2053
Size: 6361
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5368 809
Size: 507 4052 1802
Fragments arranged by size:
4052 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3808 4697 6326
Size: 54 1425 50 275 18 1978 889 1629
Cuts at: 6326 8
Size: 43
Fragments arranged by size:
1978 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3808 1537
Size: 275 1996 4090
Fragments arranged by size:
4090 1996 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2334 2628 4291 1353
Size: 981 294 1663 3423
Fragments arranged by size:
3423 1663 981 294
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4948 5322 5468 387
Size: 1064 138 3359 374 146 1280
Fragments arranged by size:
3359 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2316 2571 2604 2617 2859 3276 3567
Size: 393 684 255 33 13 242 417 291
Cuts at: 3567 4424 5021 5808 6076 1239
Size: 857 597 787 268 1524
Fragments arranged by size:
1524 857 787 684 597 417 393 291
268 255 242 33 13
EcoO109I rG'GnC_Cy
Cuts at: 2579 2579
Size: 6361
EcoRI G'AATT_C
Cuts at: 3670 4048 3670
Size: 378 5983
EcoRV GAT'ATC
Cuts at: 2593 2593
Size: 6361
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2978 2978
Size: 6361
FspI TGC'GCA
Cuts at: 4867 4867
Size: 6361
HaeII r_GCGC'y
Cuts at: 1678 5508 1678
Size: 3830 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2242 2274 2676 2708 2763
Size: 32 135 32 670 32 402 32 55
Cuts at: 2763 2795 3103 3135 3428 3460 5002 5034 5076
Size: 32 308 32 293 32 1542 32 42
Cuts at: 5076 5108 1373
Size: 32 2626
Fragments arranged by size:
2626 1542 670 402 308 293 135 55
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2758 3705 3951 669
Size: 988 281 820 947 246 3079
Fragments arranged by size:
3079 988 947 820 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6361
HpaI GTT'AAC
Cuts at: 3951 3951
Size: 6361
KpnI G_GTAC'C
Cuts at: 3686 3686
Size: 6361
MfeI C'AATT_G
Cuts at: 3960 3960
Size: 6361
MluI A'CGCG_T
Cuts at: 6237 6237
Size: 6361
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2158 2285 2424 5475 5659 1305
Size: 853 127 139 3051 184 2007
Fragments arranged by size:
3051 2007 853 184 139 127
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4539
Fragments arranged by size:
4539 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2457 3318 4319 4678 4837 518
Size: 648 1291 861 1001 359 159 2042
Fragments arranged by size:
2042 1291 1001 861 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3280 3777 4454 5602 5847 1606
Size: 1674 497 677 1148 245 2120
Fragments arranged by size:
2120 1674 1148 677 497 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3612 513
Size: 552 939 1608 3262
Fragments arranged by size:
3262 1608 939 552
NdeI CA'TA_TG
Cuts at: 387 2403 2745 387
Size: 2016 342 4003
Fragments arranged by size:
4003 2016 342
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6361
NotI GC'GGCC_GC
Cuts at: 3808 3808
Size: 6361
NsiI A_TGCA'T
Cuts at: 2745 3078 3310 2745
Size: 333 232 5796
Fragments arranged by size:
5796 333 232
NspI r_CATG'y
Cuts at: 1132 1229 2660 3719 5264 1132
Size: 97 1431 1059 1545 2229
Fragments arranged by size:
2229 1545 1431 1059 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6361
PciI A'CATG_T
Cuts at: 5260 5260
Size: 6361
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2128 3344 1108
Size: 1020 1216 4125
Fragments arranged by size:
4125 1216 1020
PmeI GTTT'AAAC
Cuts at: 3665 3665
Size: 6361
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3433 3465 4430 4462 5943 5975 3433
Size: 32 965 32 1481 32 3819
Fragments arranged by size:
3819 1481 965 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2579 2579
Size: 6361
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6361
PsiI TTA'TAA
Cuts at: 3931 3931
Size: 6361
PstI C_TGCA'G
Cuts at: 830 2754 3526 3713 830
Size: 1924 772 187 3478
Fragments arranged by size:
3478 1924 772 187
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6361
PvuII CAG'CTG
Cuts at: 3280 3280
Size: 6361
SalI G'TCGA_C
Cuts at: 1936 3703 1936
Size: 1767 4594
SapI GCTCTTCn'nnn_
Cuts at: 2628 2628
Size: 6361
SbfI CC_TGCA'GG
Cuts at: 3713 3713
Size: 6361
ScaI AGT'ACT
Cuts at: 4609 6321 4609
Size: 1712 4649
SexAI A'CCwGG_T
Cuts at: 2244 3378 2244
Size: 1134 5227
SfcI C'TryA_G
Cuts at: 826 1045 2750 3522 3709 4844 5525 5716 826
Size: 219 1705 772 187 1135 681 191 1471
Fragments arranged by size:
1705 1471 1135 772 681 219 191 187
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6361
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6361
SmaI CCC'GGG
Cuts at: 1872 3688 1872
Size: 1816 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2952 4465 5366 5628 820
Size: 197 926 1009 1513 901 262 1553
Fragments arranged by size:
1553 1513 1009 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4152 493
Size: 3659 2702
SpeI A'CTAG_T
Cuts at: 152 2519 152
Size: 2367 3994
SphI G_CATG'C
Cuts at: 1132 3719 1132
Size: 2587 3774
SspI AAT'ATT
Cuts at: 5 52 4285 5
Size: 47 4233 2081
Fragments arranged by size:
4233 2081 47
StuI AGG'CCT
Cuts at: 1517 3090 1517
Size: 1573 4788
StyI C'CwwG_G
Cuts at: 513 1065 2004 3612 3800 513
Size: 552 939 1608 188 3074
Fragments arranged by size:
3074 1608 939 552 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4388 4405 4558 4743 5188 325
Size: 1255 2808 17 153 185 445 1498
Fragments arranged by size:
2808 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2909 4607 6319
Size: 274 80 33 51 1443 932 1698 1712
Cuts at: 6319 96
Size: 138
Fragments arranged by size:
1712 1698 1443 932 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2037 2069 2590 2622 6321 6353 2037
Size: 32 521 32 3699 32 2045
Fragments arranged by size:
3699 2045 521 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6361
XbaI T'CTAG_A
Cuts at: 3697 3697
Size: 6361
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3539 3539
Size: 6361
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6361
XmaI C'CCGG_G
Cuts at: 1870 3686 1870
Size: 1816 4545
XmnI GAAnn'nnTTC
Cuts at: 4490 4490
Size: 6361
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4169 276
Size: 53 83 186 936 2635 2468
Fragments arranged by size:
2635 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SfiI SfoI SrfI SwaI