Restriction map for
pFN21AE3759
(Circular) (Six-base) MAPSORT of: pFN21AE3759.seq Check: 8968 from: 1 to: 6148
pFN21AE3759 6148 bp
With 182 enzymes: SgfI *
March 28, 2012 12:06 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3958 278
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3491 1937
Size: 1554 4594
Acc65I G'GTAC_C
Cuts at: 2559 3469 2559
Size: 910 5238
AclI AA'CG_TT
Cuts at: 1563 4275 4648 1563
Size: 2712 373 3063
Fragments arranged by size:
3063 2712 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2271 3150 4211 5595 5863 1632
Size: 639 879 1061 1384 268 1917
Fragments arranged by size:
1917 1384 1061 879 639 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5951
AflIII A'CryG_T
Cuts at: 1203 5047 6024 1203
Size: 3844 977 1327
Fragments arranged by size:
3844 1327 977
AgeI A'CCGG_T
Cuts at: 2028 4979 2028
Size: 2951 3197
AhdI GACnn_n'nnGTC
Cuts at: 4877 4877
Size: 6148
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6148
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6148
AlwNI CAG_nnn'CTG
Cuts at: 3891 5463 3891
Size: 1572 4576
ApaI G_GGCC'C
Cuts at: 2113 2113
Size: 6148
ApaLI G'TGCA_C
Cuts at: 4205 5361 4205
Size: 1156 4992
ApoI r'AATT_y
Cuts at: 1493 2318 2364 2819 3008 3142 3457 3683 3835
Size: 825 46 455 189 134 315 226 152
Cuts at: 3835 5944 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 825 455 315 226 189 152
134 46
AseI AT'TA_AT
Cuts at: 160 1032 4702 160
Size: 872 3670 1606
Fragments arranged by size:
3670 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
AvaI C'yCGr_G
Cuts at: 1870 1943 2207 3473 1870
Size: 73 264 1266 4545
Fragments arranged by size:
4545 1266 264 73
BamHI G'GATC_C
Cuts at: 2011 2416 3478 3845 2011
Size: 405 1062 367 4314
Fragments arranged by size:
4314 1062 405 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2126 2559 2576 3469 4924
Size: 325 217 627 339 433 17 893 1455
Cuts at: 4924 618
Size: 1842
Fragments arranged by size:
1842 1455 893 627 433 339 325 217
17
BanII G_rGCy'C
Cuts at: 1391 2025 2057 2113 3467 1391
Size: 634 32 56 1354 4072
Fragments arranged by size:
4072 1354 634 56 32
BbeI G_GCGC'C
Cuts at: 2130 2130
Size: 6148
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5177
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2558 2592 4339 4373 1903
Size: 34 621 34 1747 34 3678
Fragments arranged by size:
3678 1747 621 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4040 5250 4040
Size: 1210 4938
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6148
BfrBI ATG'CAT
Cuts at: 2300 2300
Size: 6148
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4759 136
Size: 107 122 71 631 605 3087 1525
Fragments arranged by size:
3087 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3196 3276 6143 3196
Size: 80 2867 3201
Fragments arranged by size:
3201 2867 80
BlpI GC'TnA_GC
Cuts at: 3565 3565
Size: 6148
Bme1580I G_kGCm'C
Cuts at: 1790 2113 4209 5365 1790
Size: 323 2096 1156 2573
Fragments arranged by size:
2573 2096 1156 323
BmgBI CAC'GTC
Cuts at: 1318 2215 1318
Size: 897 5251
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3103 3879 4837
Size: 416 53 83 399 24 1686 776 958
Cuts at: 4837 442
Size: 1753
Fragments arranged by size:
1753 1686 958 776 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6148
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2032 2032
Size: 6148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4808 1239
Size: 3569 2579
Bpu10I CC'TnA_GC
Cuts at: 2813 3392 2813
Size: 579 5569
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2370 3093 4273 5138 5436 2370
Size: 723 1180 865 298 3082
Fragments arranged by size:
3082 1180 865 723 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2227 4811 882
Size: 617 728 2584 2219
Fragments arranged by size:
2584 2219 728 617
BsaAI yAC'GTr
Cuts at: 493 1204 3939 493
Size: 711 2735 2702
Fragments arranged by size:
2735 2702 711
BsaBI GATnn'nnATC
Cuts at: 6142 6142
Size: 6148
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2036 2127 3955 4337
Size: 53 83 186 936 503 91 1828 382
Cuts at: 4337 4972 275
Size: 635 1451
Fragments arranged by size:
1828 1451 936 635 503 382 186 91
83 53
BsaWI w'CCGG_w
Cuts at: 2028 2250 4581 4979 5253 5400 5811 2028
Size: 222 2331 398 274 147 411 2365
Fragments arranged by size:
2365 2331 411 398 274 222 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5923
Fragments arranged by size:
5923 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 2078 2081 2183 2204 1186
Size: 889 3 3 102 21 5130
Fragments arranged by size:
5130 889 102 21 3 3
BseYI C'CCAG_C
Cuts at: 5351 5847 5351
Size: 496 5652
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5575
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2029 3598 4359 5387 1540
Size: 275 186 28 1569 761 1028 2301
Fragments arranged by size:
2301 1569 1028 761 275 186 28
BsiHKAI G_wGCw'C
Cuts at: 4209 4294 5365 4209
Size: 85 1071 4992
Fragments arranged by size:
4992 1071 85
BsmI GAATG_Cn'
Cuts at: 2300 2302 3659 3752 2300
Size: 2 1357 93 4696
Fragments arranged by size:
4696 1357 93 2
BsmBI CGTCTCn'nnnn_
Cuts at: 3297 3297
Size: 6148
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2057 2113 3467 4209 4294 5365
Size: 399 235 32 56 1354 742 85 1071
Cuts at: 5365 1391
Size: 2174
Fragments arranged by size:
2174 1354 1071 742 399 235 85 56
32
BspEI T'CCGG_A
Cuts at: 5811 5811
Size: 6148
BspHI T'CATG_A
Cuts at: 4037 5767 4037
Size: 1730 4418
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3503 844
Size: 2659 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2080 4035 1394
Size: 429 257 1955 3507
Fragments arranged by size:
3507 1955 429 257
BsrDI GCAATG_nn'
Cuts at: 65 1248 2929 4643 4817 65
Size: 1183 1681 1714 174 1396
Fragments arranged by size:
1714 1681 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2028 2180 4792 4979 1700
Size: 110 218 152 2612 187 2869
Fragments arranged by size:
2869 2612 218 187 152 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6148
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6148
BssSI C'ACGA_G
Cuts at: 4208 5220 4208
Size: 1012 5136
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6148
BstBI TT'CG_AA
Cuts at: 4952 4952
Size: 6148
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6148
BstXI CCAn_nnnn'nTGG
Cuts at: 3093 3093
Size: 6148
BstYI r'GATC_y
Cuts at: 1972 2011 2416 3196 3276 3478 3845 4230 4247
Size: 39 405 780 80 202 367 385 17
Cuts at: 4247 5699 5785 5797 6143 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 780 405 385 367 346 202
86 80 39 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2040 2096 2117 2262 513
Size: 552 939 36 56 21 145 4399
Fragments arranged by size:
4399 939 552 145 56 36 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5409
Fragments arranged by size:
5409 683 56
BtsI GCAGTG_nn'
Cuts at: 3664 4458 4478 3664
Size: 794 20 5334
Fragments arranged by size:
5334 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6148
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6113
DraI TTT'AAA
Cuts at: 3058 3452 3058
Size: 394 5754
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2219 5155 809
Size: 507 903 2936 1802
Fragments arranged by size:
2936 1802 903 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2530 3595 4484
Size: 54 1425 50 275 18 700 1065 889
Cuts at: 4484 6113 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1065 889 700 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3595 1537
Size: 275 1783 4090
Fragments arranged by size:
4090 1783 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2454 3305 4078 1353
Size: 1101 851 773 3423
Fragments arranged by size:
3423 1101 851 773
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4735 5109 5255 387
Size: 1064 138 3146 374 146 1280
Fragments arranged by size:
3146 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2271 3150 4211 4808 5595 5863 1239
Size: 393 639 879 1061 597 787 268 1524
Fragments arranged by size:
1524 1061 879 787 639 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3290 3290
Size: 6148
EcoRI G'AATT_C
Cuts at: 3008 3457 3835 3008
Size: 449 378 5321
Fragments arranged by size:
5321 449 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3029 3029
Size: 6148
FspI TGC'GCA
Cuts at: 4654 4654
Size: 6148
HaeII r_GCGC'y
Cuts at: 1678 2130 2142 5295 1678
Size: 452 12 3153 2531
Fragments arranged by size:
3153 2531 452 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2536 2568 3067 3099 3256
Size: 32 135 32 964 32 499 32 157
Cuts at: 3256 3288 4789 4821 4863 4895 1373
Size: 32 1501 32 42 32 2626
Fragments arranged by size:
2626 1501 964 499 157 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2474 3492 3738 669
Size: 988 281 536 1018 246 3079
Fragments arranged by size:
3079 1018 988 536 281 246
HindIII A'AGCT_T
Cuts at: 748 2597 748
Size: 1849 4299
HpaI GTT'AAC
Cuts at: 3738 3738
Size: 6148
KasI G'GCGC_C
Cuts at: 2126 2126
Size: 6148
KpnI G_GTAC'C
Cuts at: 2563 3473 2563
Size: 910 5238
MfeI C'AATT_G
Cuts at: 3030 3418 3747 3030
Size: 388 329 5431
Fragments arranged by size:
5431 388 329
MluI A'CGCG_T
Cuts at: 6024 6024
Size: 6148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2341 3359 5262 5446 1305
Size: 1036 1018 1903 184 2007
Fragments arranged by size:
2007 1903 1036 1018 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4326
Fragments arranged by size:
4326 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3066 4106 4465 4624 518
Size: 648 1900 1040 359 159 2042
Fragments arranged by size:
2042 1900 1040 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2042 2089 2098 2119 2264 3564 4241 5389
Size: 436 47 9 21 145 1300 677 1148
Cuts at: 5389 5634 1606
Size: 245 2120
Fragments arranged by size:
2120 1300 1148 677 436 245 145 47
21 9
NaeI GCC'GGC
Cuts at: 2182 2182
Size: 6148
NarI GG'CG_CC
Cuts at: 2127 2127
Size: 6148
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4657
Fragments arranged by size:
4657 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6148
NgoMIV G'CCGG_C
Cuts at: 2180 2180
Size: 6148
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6148
NotI GC'GGCC_GC
Cuts at: 3595 3595
Size: 6148
NsiI A_TGCA'T
Cuts at: 2302 2302
Size: 6148
NspI r_CATG'y
Cuts at: 1132 1229 3506 5051 1132
Size: 97 2277 1545 2229
Fragments arranged by size:
2277 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6148
PciI A'CATG_T
Cuts at: 5047 5047
Size: 6148
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6148
PfoI T'CCnGG_A
Cuts at: 2225 2225
Size: 6148
PmeI GTTT'AAAC
Cuts at: 3452 3452
Size: 6148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4217 4249 5730 5762 4217
Size: 32 1481 32 4603
Fragments arranged by size:
4603 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6148
PsiI TTA'TAA
Cuts at: 3718 3718
Size: 6148
PspOMI G'GGCC_C
Cuts at: 2109 2109
Size: 6148
PstI C_TGCA'G
Cuts at: 830 3500 830
Size: 2670 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6148
SacII CC_GC'GG
Cuts at: 2043 2099 2120 2265 2043
Size: 56 21 145 5926
Fragments arranged by size:
5926 145 56 21
SalI G'TCGA_C
Cuts at: 1936 3490 1936
Size: 1554 4594
SapI GCTCTTCn'nnn_
Cuts at: 2454 2454
Size: 6148
SbfI CC_TGCA'GG
Cuts at: 3500 3500
Size: 6148
ScaI AGT'ACT
Cuts at: 4396 6108 4396
Size: 1712 4436
SfcI C'TryA_G
Cuts at: 826 1045 3496 4631 5312 5503 826
Size: 219 2451 1135 681 191 1471
Fragments arranged by size:
2451 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2128 2128
Size: 6148
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6148
SmaI CCC'GGG
Cuts at: 1872 2209 3475 1872
Size: 337 1266 4545
Fragments arranged by size:
4545 1266 337
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2385 3108 4252 5153 5415 820
Size: 197 926 442 723 1144 901 262 1553
Fragments arranged by size:
1553 1144 926 901 723 442 262 197
SnaBI TAC'GTA
Cuts at: 493 3939 493
Size: 3446 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6148
SphI G_CATG'C
Cuts at: 1132 3506 1132
Size: 2374 3774
SspI AAT'ATT
Cuts at: 5 52 2807 2848 4072 5
Size: 47 2755 41 1224 2081
Fragments arranged by size:
2755 2081 1224 47 41
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6148
StyI C'CwwG_G
Cuts at: 513 1065 2004 3587 513
Size: 552 939 1583 3074
Fragments arranged by size:
3074 1583 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4175 4192 4345 4530 4975 325
Size: 1255 2595 17 153 185 445 1498
Fragments arranged by size:
2595 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3328 4394 6106
Size: 274 80 33 51 1443 1351 1066 1712
Cuts at: 6106 96
Size: 138
Fragments arranged by size:
1712 1443 1351 1066 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6108 6140 6108
Size: 32 6116
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6148
XbaI T'CTAG_A
Cuts at: 3484 3484
Size: 6148
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6148
XmaI C'CCGG_G
Cuts at: 1870 2207 3473 1870
Size: 337 1266 4545
Fragments arranged by size:
4545 1266 337
XmnI GAAnn'nnTTC
Cuts at: 2300 4277 2300
Size: 1977 4171
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3956 276
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BsiWI
BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI
NruI PacI PmlI PpuMI PspXI PsrI PvuII RsrII
SacI SanDI SexAI SfiI SrfI SwaI XcmI