Restriction map for
pFN21AE3758
(Circular) (Six-base) MAPSORT of: pFN21AE3758.seq Check: 1675 from: 1 to: 5998
pFN21AE3758 5998 bp
With 182 enzymes: SgfI *
March 28, 2012 12:04 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3808 278
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3341 1937
Size: 1404 4594
Acc65I G'GTAC_C
Cuts at: 3319 3319
Size: 5998
AclI AA'CG_TT
Cuts at: 1563 4125 4498 1563
Size: 2562 373 3063
Fragments arranged by size:
3063 2562 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2024 2317 2640 4061 5445 5713 1632
Size: 392 293 323 1421 1384 268 1917
Fragments arranged by size:
1917 1421 1384 392 323 293 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5801
AflIII A'CryG_T
Cuts at: 1203 4897 5874 1203
Size: 3694 977 1327
Fragments arranged by size:
3694 1327 977
AgeI A'CCGG_T
Cuts at: 2602 4829 2602
Size: 2227 3771
AhdI GACnn_n'nnGTC
Cuts at: 4727 4727
Size: 5998
AleI CACnn'nnGTG
Cuts at: 1166 2996 1166
Size: 1830 4168
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5998
AlwNI CAG_nnn'CTG
Cuts at: 2047 3741 5313 2047
Size: 1694 1572 2732
Fragments arranged by size:
2732 1694 1572
ApaLI G'TGCA_C
Cuts at: 4055 5211 4055
Size: 1156 4842
ApoI r'AATT_y
Cuts at: 1493 2573 2887 3307 3533 3685 5794 1493
Size: 1080 314 420 226 152 2109 1697
Fragments arranged by size:
2109 1697 1080 420 314 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4552 160
Size: 872 3520 1606
Fragments arranged by size:
3520 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
AvaI C'yCGr_G
Cuts at: 1870 1943 3323 1870
Size: 73 1380 4545
Fragments arranged by size:
4545 1380 73
BamHI G'GATC_C
Cuts at: 2284 3328 3695 2284
Size: 1044 367 4587
Fragments arranged by size:
4587 1044 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2399 2986 3319 4774 618
Size: 325 217 627 612 587 333 1455 1842
Fragments arranged by size:
1842 1455 627 612 587 333 325 217
BanII G_rGCy'C
Cuts at: 1391 2191 2556 3041 3317 1391
Size: 800 365 485 276 4072
Fragments arranged by size:
4072 800 485 365 276
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3299 928
Size: 971 1400 3627
Fragments arranged by size:
3627 1400 971
BbvCI CC'TCA_GC
Cuts at: 2391 2391
Size: 5998
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4189 4223 1903
Size: 34 2252 34 3678
Fragments arranged by size:
3678 2252 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3134 3890 5100 3134
Size: 756 1210 4032
Fragments arranged by size:
4032 1210 756
BclI T'GATC_A
Cuts at: 1548 3000 1548
Size: 1452 4546
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4609 136
Size: 107 122 71 631 605 2937 1525
Fragments arranged by size:
2937 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5993 5993
Size: 5998
BlpI GC'TnA_GC
Cuts at: 3415 3415
Size: 5998
Bme1580I G_kGCm'C
Cuts at: 1790 4059 5215 1790
Size: 2269 1156 2573
Fragments arranged by size:
2573 2269 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5998
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3035 3729 4687
Size: 416 53 83 399 24 1618 694 958
Cuts at: 4687 442
Size: 1753
Fragments arranged by size:
1753 1618 958 694 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5998
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3060 3060
Size: 5998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2918 3103 3154 4658 1239
Size: 1679 185 51 1504 2579
Fragments arranged by size:
2579 1679 1504 185 51
Bpu10I CC'TnA_GC
Cuts at: 2391 2391
Size: 5998
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4123 4988 5286 4123
Size: 865 298 4835
Fragments arranged by size:
4835 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4661 882
Size: 617 3162 2219
Fragments arranged by size:
3162 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3789 493
Size: 711 2585 2702
Fragments arranged by size:
2702 2585 711
BsaBI GATnn'nnATC
Cuts at: 5992 5992
Size: 5998
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3805 4187 4822 275
Size: 53 83 186 936 2272 382 635 1451
Fragments arranged by size:
2272 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2602 4431 4829 5103 5250 5661 2602
Size: 1829 398 274 147 411 2939
Fragments arranged by size:
2939 1829 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5773
Fragments arranged by size:
5773 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2464 2535 1186
Size: 1278 71 4649
Fragments arranged by size:
4649 1278 71
BseYI C'CCAG_C
Cuts at: 5201 5697 5201
Size: 496 5502
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5425
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3448 4209 5237 1540
Size: 275 186 1447 761 1028 2301
Fragments arranged by size:
2301 1447 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4059 4144 5215 4059
Size: 85 1071 4842
Fragments arranged by size:
4842 1071 85
BsmI GAATG_Cn'
Cuts at: 3509 3602 3509
Size: 93 5905
BsmBI CGTCTCn'nnnn_
Cuts at: 2138 2138
Size: 5998
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2191 2556 3041 3317 4059 4144 5215
Size: 399 401 365 485 276 742 85 1071
Cuts at: 5215 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 485 401 399 365 276
85
BspEI T'CCGG_A
Cuts at: 5661 5661
Size: 5998
BspHI T'CATG_A
Cuts at: 3887 5617 3887
Size: 1730 4268
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3353 844
Size: 2509 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3885 1394
Size: 429 2062 3507
Fragments arranged by size:
3507 2062 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2425 4493 4667 65
Size: 1183 1177 2068 174 1396
Fragments arranged by size:
2068 1396 1183 1177 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2602 4642 4829 1700
Size: 110 792 2040 187 2869
Fragments arranged by size:
2869 2040 792 187 110
BsrGI T'GTAC_A
Cuts at: 96 2247 96
Size: 2151 3847
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5998
BssSI C'ACGA_G
Cuts at: 3004 4058 5070 3004
Size: 1054 1012 3932
Fragments arranged by size:
3932 1054 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5998
BstBI TT'CG_AA
Cuts at: 4802 4802
Size: 5998
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5998
BstXI CCAn_nnnn'nTGG
Cuts at: 2362 2362
Size: 5998
BstYI r'GATC_y
Cuts at: 1972 2284 2957 3328 3695 4080 4097 5549 5635
Size: 312 673 371 367 385 17 1452 86
Cuts at: 5635 5647 5993 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 673 385 371 367 346 312
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2547 2547
Size: 5998
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4507
Fragments arranged by size:
4507 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5259
Fragments arranged by size:
5259 683 56
BtsI GCAGTG_nn'
Cuts at: 3016 3514 4308 4328 3016
Size: 498 794 20 4686
Fragments arranged by size:
4686 794 498 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5998
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2277 2312 529
Size: 35 1713 35 4215
Fragments arranged by size:
4215 1713 35 35
DraI TTT'AAA
Cuts at: 3302 3302
Size: 5998
DraIII CAC_nnn'GTG
Cuts at: 2025 2025
Size: 5998
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5005 809
Size: 507 3689 1802
Fragments arranged by size:
3689 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3445 4334 5963
Size: 54 1425 50 275 18 1615 889 1629
Cuts at: 5963 8
Size: 43
Fragments arranged by size:
1629 1615 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3445 1537
Size: 275 1633 4090
Fragments arranged by size:
4090 1633 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2115 3928 1353
Size: 762 1813 3423
Fragments arranged by size:
3423 1813 762
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4585 4959 5105 387
Size: 1064 138 2996 374 146 1280
Fragments arranged by size:
2996 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2024 2317 2640 2918 3103 3154 4061
Size: 393 392 293 323 278 185 51 907
Cuts at: 4061 4658 5445 5713 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 907 787 597 393 392 323 293
278 268 185 51
EcoRI G'AATT_C
Cuts at: 3307 3685 3307
Size: 378 5620
FspI TGC'GCA
Cuts at: 4504 4504
Size: 5998
HaeII r_GCGC'y
Cuts at: 1678 5145 1678
Size: 3467 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2497 2525 2529 2557 2876
Size: 32 135 32 925 28 4 28 319
Cuts at: 2876 2908 2998 3030 4639 4671 4713 4745 1373
Size: 32 90 32 1609 32 42 32 2626
Fragments arranged by size:
2626 1609 925 319 135 90 42 32
32 32 32 32 32 28 28 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3342 3588 669
Size: 988 281 1404 246 3079
Fragments arranged by size:
3079 1404 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2105 2486 2673 748
Size: 1357 381 187 4073
Fragments arranged by size:
4073 1357 381 187
HpaI GTT'AAC
Cuts at: 3588 3588
Size: 5998
KpnI G_GTAC'C
Cuts at: 3323 3323
Size: 5998
MfeI C'AATT_G
Cuts at: 2631 3597 2631
Size: 966 5032
MluI A'CGCG_T
Cuts at: 5874 5874
Size: 5998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2060 5112 5296 1305
Size: 755 3052 184 2007
Fragments arranged by size:
3052 2007 755 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4176
Fragments arranged by size:
4176 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2996 3956 4315 4474 518
Size: 648 1830 960 359 159 2042
Fragments arranged by size:
2042 1830 960 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3414 4091 5239 5484 1606
Size: 1808 677 1148 245 2120
Fragments arranged by size:
2120 1808 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4507
Fragments arranged by size:
4507 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5998
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5998
NotI GC'GGCC_GC
Cuts at: 3445 3445
Size: 5998
NspI r_CATG'y
Cuts at: 1132 1229 3356 4901 1132
Size: 97 2127 1545 2229
Fragments arranged by size:
2229 2127 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5998
PciI A'CATG_T
Cuts at: 4897 4897
Size: 5998
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2677 1108
Size: 1569 4429
PmeI GTTT'AAAC
Cuts at: 3302 3302
Size: 5998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4067 4099 5580 5612 4067
Size: 32 1481 32 4453
Fragments arranged by size:
4453 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5998
PsiI TTA'TAA
Cuts at: 3568 3568
Size: 5998
PstI C_TGCA'G
Cuts at: 830 2052 3350 830
Size: 1222 1298 3478
Fragments arranged by size:
3478 1298 1222
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5998
SalI G'TCGA_C
Cuts at: 1936 3340 1936
Size: 1404 4594
SbfI CC_TGCA'GG
Cuts at: 3350 3350
Size: 5998
ScaI AGT'ACT
Cuts at: 3139 4246 5958 3139
Size: 1107 1712 3179
Fragments arranged by size:
3179 1712 1107
SfcI C'TryA_G
Cuts at: 826 1045 2048 3051 3287 3346 4481 5162 5353
Size: 219 1003 1003 236 59 1135 681 191
Cuts at: 5353 826
Size: 1471
Fragments arranged by size:
1471 1135 1003 1003 681 236 219 191
59
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5998
SmaI CCC'GGG
Cuts at: 1872 3325 1872
Size: 1453 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4102 5003 5265 820
Size: 197 926 2159 901 262 1553
Fragments arranged by size:
2159 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3789 493
Size: 3296 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5998
SphI G_CATG'C
Cuts at: 1132 3356 1132
Size: 2224 3774
SspI AAT'ATT
Cuts at: 5 52 3922 5
Size: 47 3870 2081
Fragments arranged by size:
3870 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5998
StyI C'CwwG_G
Cuts at: 513 1065 2004 3437 513
Size: 552 939 1433 3074
Fragments arranged by size:
3074 1433 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2779 2844 3004 3103 4025 4042 4195
Size: 1255 1199 65 160 99 922 17 153
Cuts at: 4195 4380 4825 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1199 922 445 185 160 153
99 65 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2247 3137 3211
Size: 274 80 33 51 1443 270 890 74
Cuts at: 3211 4244 5956 96
Size: 1033 1712 138
Fragments arranged by size:
1712 1443 1033 890 274 270 138 80
74 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2296 2328 5958 5990 2296
Size: 32 3630 32 2304
Fragments arranged by size:
3630 2304 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5998
XbaI T'CTAG_A
Cuts at: 3334 3334
Size: 5998
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5998
XmaI C'CCGG_G
Cuts at: 1870 3323 1870
Size: 1453 4545
XmnI GAAnn'nnTTC
Cuts at: 2806 4127 2806
Size: 1321 4677
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3806 276
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BstZ17I EcoICRI EcoNI EcoO109I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI