Restriction map for
pFN21AE3757
(Circular) (Six-base) MAPSORT of: pFN21AE3757.seq Check: 7588 from: 1 to: 5890
pFN21AE3757 5890 bp
With 182 enzymes: SgfI *
March 28, 2012 12:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2357 2357
Size: 5890
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2736 3700 278
Size: 53 83 186 936 1200 964 2468
Fragments arranged by size:
2468 1200 964 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2736 3233 1937
Size: 799 497 4594
Fragments arranged by size:
4594 799 497
Acc65I G'GTAC_C
Cuts at: 2529 3211 2529
Size: 682 5208
AclI AA'CG_TT
Cuts at: 1563 4017 4390 1563
Size: 2454 373 3063
Fragments arranged by size:
3063 2454 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3953 5337 5605 1632
Size: 2321 1384 268 1917
Fragments arranged by size:
2321 1917 1384 268
AfeI AGC'GCT
Cuts at: 2037 2292 2037
Size: 255 5635
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5693
AflIII A'CryG_T
Cuts at: 1203 4789 5766 1203
Size: 3586 977 1327
Fragments arranged by size:
3586 1327 977
AgeI A'CCGG_T
Cuts at: 4721 4721
Size: 5890
AhdI GACnn_n'nnGTC
Cuts at: 4619 4619
Size: 5890
AleI CACnn'nnGTG
Cuts at: 1166 2882 1166
Size: 1716 4174
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5890
AlwNI CAG_nnn'CTG
Cuts at: 2250 3633 5205 2250
Size: 1383 1572 2935
Fragments arranged by size:
2935 1572 1383
ApaLI G'TGCA_C
Cuts at: 2435 3947 5103 2435
Size: 1512 1156 3222
Fragments arranged by size:
3222 1512 1156
ApoI r'AATT_y
Cuts at: 1493 3127 3199 3425 3577 5686 1493
Size: 1634 72 226 152 2109 1697
Fragments arranged by size:
2109 1697 1634 226 152 72
AseI AT'TA_AT
Cuts at: 160 1032 4444 160
Size: 872 3412 1606
Fragments arranged by size:
3412 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5890
AvaI C'yCGr_G
Cuts at: 1870 1943 2075 2201 2796 3215 1870
Size: 73 132 126 595 419 4545
Fragments arranged by size:
4545 595 419 132 126 73
BamHI G'GATC_C
Cuts at: 3220 3587 3220
Size: 367 5523
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2121 2529 3211 4666 618
Size: 325 217 627 334 408 682 1455 1842
Fragments arranged by size:
1842 1455 682 627 408 334 325 217
BanII G_rGCy'C
Cuts at: 1391 2049 2280 2288 2344 3209 1391
Size: 658 231 8 56 865 4072
Fragments arranged by size:
4072 865 658 231 56 8
BbeI G_GCGC'C
Cuts at: 2125 2125
Size: 5890
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4919
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4081 4115 1903
Size: 34 2144 34 3678
Fragments arranged by size:
3678 2144 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2920 3782 4992 2920
Size: 862 1210 3818
Fragments arranged by size:
3818 1210 862
BclI T'GATC_A
Cuts at: 1548 2986 1548
Size: 1438 4452
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2168 2177
Size: 107 122 71 631 605 337 159 9
Cuts at: 2177 4501 136
Size: 2324 1525
Fragments arranged by size:
2324 1525 631 605 337 159 122 107
71 9
BglII A'GATC_T
Cuts at: 5885 5885
Size: 5890
BlpI GC'TnA_GC
Cuts at: 2287 2939 3307 2287
Size: 652 368 4870
Fragments arranged by size:
4870 652 368
Bme1580I G_kGCm'C
Cuts at: 1790 2210 2439 3951 5107 1790
Size: 420 229 1512 1156 2573
Fragments arranged by size:
2573 1512 1156 420 229
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5890
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2874 3193 3621
Size: 416 53 83 399 24 1457 319 428
Cuts at: 3621 4579 442
Size: 958 1753
Fragments arranged by size:
1753 1457 958 428 416 399 319 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5890
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2861 4550 1239
Size: 1622 1689 2579
Fragments arranged by size:
2579 1689 1622
Bpu10I CC'TnA_GC
Cuts at: 3040 3040
Size: 5890
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2884 4015 4880 5178 2884
Size: 1131 865 298 3596
Fragments arranged by size:
3596 1131 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2663 4553 882
Size: 617 1164 1890 2219
Fragments arranged by size:
2219 1890 1164 617
BsaAI yAC'GTr
Cuts at: 493 1204 2149 3681 493
Size: 711 945 1532 2702
Fragments arranged by size:
2702 1532 945 711
BsaBI GATnn'nnATC
Cuts at: 5884 5884
Size: 5890
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2122 2580 2733 3697
Size: 53 83 186 936 589 458 153 964
Cuts at: 3697 4079 4714 275
Size: 382 635 1451
Fragments arranged by size:
1451 964 936 635 589 458 382 186
153 83 53
BsaWI w'CCGG_w
Cuts at: 2393 4323 4721 4995 5142 5553 2393
Size: 1930 398 274 147 411 2730
Fragments arranged by size:
2730 1930 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2699 2729 2939 2969 1178
Size: 30 165 30 1296 30 210 30 4099
Fragments arranged by size:
4099 1296 210 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3068 1186
Size: 1882 4008
BseYI C'CCAG_C
Cuts at: 2327 2370 5093 5589 2327
Size: 43 2723 496 2628
Fragments arranged by size:
2723 2628 496 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2249 2705 1163
Size: 573 513 456 4348
Fragments arranged by size:
4348 573 513 456
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2013 2310 2590 3340 4101 5129
Size: 275 186 12 297 280 750 761 1028
Cuts at: 5129 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 750 297 280 275 186
12
BsiHKAI G_wGCw'C
Cuts at: 2049 2280 2439 2847 3951 4036 5107 2049
Size: 231 159 408 1104 85 1071 2832
Fragments arranged by size:
2832 1104 1071 408 231 159 85
BsmI GAATG_Cn'
Cuts at: 3401 3494 3401
Size: 93 5797
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2210 2280 2288 2344 2439 2847
Size: 399 259 161 70 8 56 95 408
Cuts at: 2847 3209 3951 4036 5107 1391
Size: 362 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 408 399 362 259 161
95 85 70 56 8
BspEI T'CCGG_A
Cuts at: 5553 5553
Size: 5890
BspHI T'CATG_A
Cuts at: 3779 5509 3779
Size: 1730 4160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2357 3245 844
Size: 1513 888 3489
Fragments arranged by size:
3489 1513 888
BsrBI CCG'CTC
Cuts at: 1394 1823 2028 3777 1394
Size: 429 205 1749 3507
Fragments arranged by size:
3507 1749 429 205
BsrDI GCAATG_nn'
Cuts at: 65 1248 2829 4385 4559 65
Size: 1183 1581 1556 174 1396
Fragments arranged by size:
1581 1556 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2109 2257 2582 4534 4721 1700
Size: 110 299 148 325 1952 187 2869
Fragments arranged by size:
2869 1952 325 299 187 148 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5890
BssHII G'CGCG_C
Cuts at: 1925 2484 1925
Size: 559 5331
BssSI C'ACGA_G
Cuts at: 3950 4962 3950
Size: 1012 4878
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2088 794
Size: 1294 4596
BstBI TT'CG_AA
Cuts at: 4694 4694
Size: 5890
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5890
BstXI CCAn_nnnn'nTGG
Cuts at: 2398 2398
Size: 5890
BstYI r'GATC_y
Cuts at: 1972 3220 3587 3972 3989 5441 5527 5539 5885
Size: 1248 367 385 17 1452 86 12 346
Cuts at: 5885 1972
Size: 1977
Fragments arranged by size:
1977 1452 1248 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3051 3051
Size: 5890
BtgI C'CryG_G
Cuts at: 513 1065 2004 3075 513
Size: 552 939 1071 3328
Fragments arranged by size:
3328 1071 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2300 487
Size: 683 56 1074 4077
Fragments arranged by size:
4077 1074 683 56
BtsI GCAGTG_nn'
Cuts at: 2814 3406 4200 4220 2814
Size: 592 794 20 4484
Fragments arranged by size:
4484 794 592 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5890
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5855
DraI TTT'AAA
Cuts at: 3194 3194
Size: 5890
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4897 809
Size: 507 3581 1802
Fragments arranged by size:
3581 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2104 2143
Size: 54 1425 50 275 18 180 94 39
Cuts at: 2143 2169 2307 2617 2917 3337 4226 5855 8
Size: 26 138 310 300 420 889 1629 43
Fragments arranged by size:
1629 1425 889 420 310 300 275 180
138 94 54 50 43 39 26 18
EagI C'GGCC_G
Cuts at: 1537 1812 2010 2307 3337 1537
Size: 275 198 297 1030 4090
Fragments arranged by size:
4090 1030 297 275 198
EarI CTCTTCn'nnn_
Cuts at: 1353 3138 3820 1353
Size: 1785 682 3423
Fragments arranged by size:
3423 1785 682
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2075 4477 4851 4997 387
Size: 1064 138 486 2402 374 146 1280
Fragments arranged by size:
2402 1280 1064 486 374 146 138
EcoICRI GAG'CTC
Cuts at: 2047 2278 2047
Size: 231 5659
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2861 3953 4550 5337 5605 1239
Size: 393 1229 1092 597 787 268 1524
Fragments arranged by size:
1524 1229 1092 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2376 2715 2752 2376
Size: 339 37 5514
Fragments arranged by size:
5514 339 37
EcoRI G'AATT_C
Cuts at: 3127 3199 3577 3127
Size: 72 378 5440
Fragments arranged by size:
5440 378 72
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2107 2771 2107
Size: 664 5226
FspI TGC'GCA
Cuts at: 4396 4396
Size: 5890
HaeII r_GCGC'y
Cuts at: 1678 2039 2125 2294 2334 2405 5037 1678
Size: 361 86 169 40 71 2632 2531
Fragments arranged by size:
2632 2531 361 169 86 71 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2662 2694 2699 2731 2971
Size: 32 135 32 1090 32 5 32 240
Cuts at: 2971 3003 3137 3169 4531 4563 4605 4637 1373
Size: 32 134 32 1362 32 42 32 2626
Fragments arranged by size:
2626 1362 1090 240 135 134 42 32
32 32 32 32 32 32 32 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2386 2737 3181 3234 3480 669
Size: 988 281 448 351 444 53 246 3079
Fragments arranged by size:
3079 988 448 444 351 281 246 53
HindIII A'AGCT_T
Cuts at: 748 2627 748
Size: 1879 4011
HpaI GTT'AAC
Cuts at: 3480 3480
Size: 5890
KasI G'GCGC_C
Cuts at: 2121 2121
Size: 5890
KpnI G_GTAC'C
Cuts at: 2533 3215 2533
Size: 682 5208
MfeI C'AATT_G
Cuts at: 3489 3489
Size: 5890
MluI A'CGCG_T
Cuts at: 5766 5766
Size: 5890
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5004 5188 1305
Size: 3699 184 2007
Fragments arranged by size:
3699 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2106 2619 10
Size: 54 1425 343 274 513 3281
Fragments arranged by size:
3281 1425 513 343 274 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2600 2882 3848 4207 4366 518
Size: 648 1434 282 966 359 159 2042
Fragments arranged by size:
2042 1434 966 648 359 282 159
MspA1I CmG'CkG
Cuts at: 1606 2119 2975 3306 3983 5131 5376 1606
Size: 513 856 331 677 1148 245 2120
Fragments arranged by size:
2120 1148 856 677 513 331 245
NaeI GCC'GGC
Cuts at: 2584 2584
Size: 5890
NarI GG'CG_CC
Cuts at: 2122 2122
Size: 5890
NcoI C'CATG_G
Cuts at: 513 1065 2004 3075 513
Size: 552 939 1071 3328
Fragments arranged by size:
3328 1071 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5890
NgoMIV G'CCGG_C
Cuts at: 2582 2582
Size: 5890
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5890
NotI GC'GGCC_GC
Cuts at: 3337 3337
Size: 5890
NspI r_CATG'y
Cuts at: 1132 1229 3248 4793 1132
Size: 97 2019 1545 2229
Fragments arranged by size:
2229 2019 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2857 1348
Size: 1509 4381
PciI A'CATG_T
Cuts at: 4789 4789
Size: 5890
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5890
PfoI T'CCnGG_A
Cuts at: 2727 2838 2727
Size: 111 5779
PmeI GTTT'AAAC
Cuts at: 3194 3194
Size: 5890
PmlI CAC'GTG
Cuts at: 2149 2149
Size: 5890
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3959 3991 5472 5504 3959
Size: 32 1481 32 4345
Fragments arranged by size:
4345 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2376 2715 2752 2376
Size: 339 37 5514
Fragments arranged by size:
5514 339 37
PshAI GACnn'nnGTC
Cuts at: 1613 2381 1613
Size: 768 5122
PsiI TTA'TAA
Cuts at: 3460 3460
Size: 5890
PspXI vC'TCGA_Gb
Cuts at: 2075 2201 2075
Size: 126 5764
PstI C_TGCA'G
Cuts at: 830 2219 3242 830
Size: 1389 1023 3478
Fragments arranged by size:
3478 1389 1023
PvuI CG_AT'CG
Cuts at: 2001 2590 2001
Size: 589 5301
SacI G_AGCT'C
Cuts at: 2049 2280 2049
Size: 231 5659
SalI G'TCGA_C
Cuts at: 1936 2735 3232 1936
Size: 799 497 4594
Fragments arranged by size:
4594 799 497
SanDI GG'GwC_CC
Cuts at: 2376 2715 2376
Size: 339 5551
SbfI CC_TGCA'GG
Cuts at: 3242 3242
Size: 5890
ScaI AGT'ACT
Cuts at: 4138 5850 4138
Size: 1712 4178
SfcI C'TryA_G
Cuts at: 826 1045 2215 3238 4373 5054 5245 826
Size: 219 1170 1023 1135 681 191 1471
Fragments arranged by size:
1471 1170 1135 1023 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2168 2168
Size: 5890
SfoI GGC'GCC
Cuts at: 2123 2123
Size: 5890
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5890
SgrAI Cr'CCGG_yG
Cuts at: 1700 2582 1700
Size: 882 5008
SmaI CCC'GGG
Cuts at: 1872 3217 1872
Size: 1345 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2075 2201 2899 3994 4895 5157
Size: 197 926 132 126 698 1095 901 262
Cuts at: 5157 820
Size: 1553
Fragments arranged by size:
1553 1095 926 901 698 262 197 132
126
SnaBI TAC'GTA
Cuts at: 493 3681 493
Size: 3188 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5890
SphI G_CATG'C
Cuts at: 1132 3248 1132
Size: 2116 3774
SspI AAT'ATT
Cuts at: 5 52 3814 5
Size: 47 3762 2081
Fragments arranged by size:
3762 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5890
StyI C'CwwG_G
Cuts at: 513 1065 2004 2657 3075 3120 3329 513
Size: 552 939 653 418 45 209 3074
Fragments arranged by size:
3074 939 653 552 418 209 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3917 3934 4087 4272 4717 325
Size: 1255 2337 17 153 185 445 1498
Fragments arranged by size:
2337 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4136 5848 96
Size: 274 80 33 51 1443 2159 1712 138
Fragments arranged by size:
2159 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5850 5882 5850
Size: 32 5858
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5890
XbaI T'CTAG_A
Cuts at: 3226 3226
Size: 5890
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2836 2836
Size: 5890
XhoI C'TCGA_G
Cuts at: 1943 2075 2201 1943
Size: 132 126 5632
Fragments arranged by size:
5632 132 126
XmaI C'CCGG_G
Cuts at: 1870 3215 1870
Size: 1345 4545
XmnI GAAnn'nnTTC
Cuts at: 4019 4019
Size: 5890
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2734 3698 276
Size: 53 83 186 936 1200 964 2468
Fragments arranged by size:
2468 1200 964 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI SacI SalI SanDI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI
NruI NsiI PacI PspOMI PsrI PvuII RsrII SacII
SapI SexAI SrfI SwaI