Restriction map for
pFN21AE3756
(Circular) (Six-base) MAPSORT of: pFN21AE3756.seq Check: 7563 from: 1 to: 5821
pFN21AE3756 5821 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2406 2406
Size: 5821
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3631 278
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2088 2628 2772 3164 1937
Size: 151 540 144 392 4594
Fragments arranged by size:
4594 540 392 151 144
Acc65I G'GTAC_C
Cuts at: 3142 3142
Size: 5821
AclI AA'CG_TT
Cuts at: 1563 3948 4321 1563
Size: 2385 373 3063
Fragments arranged by size:
3063 2385 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2524 3884 5268 5536 1632
Size: 892 1360 1384 268 1917
Fragments arranged by size:
1917 1384 1360 892 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5624
AflIII A'CryG_T
Cuts at: 1203 2184 2316 4720 5697 1203
Size: 981 132 2404 977 1327
Fragments arranged by size:
2404 1327 981 977 132
AgeI A'CCGG_T
Cuts at: 4652 4652
Size: 5821
AhdI GACnn_n'nnGTC
Cuts at: 4550 4550
Size: 5821
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5821
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2585 2942 1184
Size: 1401 357 4063
Fragments arranged by size:
4063 1401 357
AlwNI CAG_nnn'CTG
Cuts at: 3055 3564 5136 3055
Size: 509 1572 3740
Fragments arranged by size:
3740 1572 509
ApaLI G'TGCA_C
Cuts at: 2162 2375 2480 3878 5034 2162
Size: 213 105 1398 1156 2949
Fragments arranged by size:
2949 1398 1156 213 105
ApoI r'AATT_y
Cuts at: 1493 3130 3356 3508 5617 1493
Size: 1637 226 152 2109 1697
Fragments arranged by size:
2109 1697 1637 226 152
AscI GG'CGCG_CC
Cuts at: 2660 2660
Size: 5821
AseI AT'TA_AT
Cuts at: 160 1032 4375 160
Size: 872 3343 1606
Fragments arranged by size:
3343 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
AvaI C'yCGr_G
Cuts at: 1870 1943 2513 3146 1870
Size: 73 570 633 4545
Fragments arranged by size:
4545 633 570 73
BamHI G'GATC_C
Cuts at: 3151 3518 3151
Size: 367 5454
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2297 2864 3142 4597 618
Size: 325 217 627 510 567 278 1455 1842
Fragments arranged by size:
1842 1455 627 567 510 325 278 217
BanII G_rGCy'C
Cuts at: 1391 2115 3140 1391
Size: 724 1025 4072
Fragments arranged by size:
4072 1025 724
BbeI G_GCGC'C
Cuts at: 2301 2868 2301
Size: 567 5254
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2134 2638 2702 3078 928
Size: 971 235 504 64 376 3671
Fragments arranged by size:
3671 971 504 376 235 64
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4012 4046 1903
Size: 34 2075 34 3678
Fragments arranged by size:
3678 2075 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3713 4923 3713
Size: 1210 4611
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5821
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4432 136
Size: 107 122 71 631 605 2760 1525
Fragments arranged by size:
2760 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5816 5816
Size: 5821
BlpI GC'TnA_GC
Cuts at: 3238 3238
Size: 5821
Bme1580I G_kGCm'C
Cuts at: 1790 2166 2379 2469 2484 2751 2942 3882 5038
Size: 376 213 90 15 267 191 940 1156
Cuts at: 5038 1790
Size: 2573
Fragments arranged by size:
2573 1156 940 376 267 213 191 90
15
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5821
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3552 4510 442
Size: 416 53 83 399 24 2135 958 1753
Fragments arranged by size:
2135 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2132 1056
Size: 1076 4745
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4481 1239
Size: 3242 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3946 4811 5109 3946
Size: 865 298 4658
Fragments arranged by size:
4658 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4484 882
Size: 617 2985 2219
Fragments arranged by size:
2985 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3612 493
Size: 711 2408 2702
Fragments arranged by size:
2702 2408 711
BsaBI GATnn'nnATC
Cuts at: 2345 5815 2345
Size: 3470 2351
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2298 2670 2865 3024
Size: 53 83 186 936 765 372 195 159
Cuts at: 3024 3628 4010 4645 275
Size: 604 382 635 1451
Fragments arranged by size:
1451 936 765 635 604 382 372 195
186 159 83 53
BsaWI w'CCGG_w
Cuts at: 4254 4652 4926 5073 5484 4254
Size: 398 274 147 411 4591
Fragments arranged by size:
4591 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5596
Fragments arranged by size:
5596 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2011 1186
Size: 825 4996
BseYI C'CCAG_C
Cuts at: 2596 5024 5520 2596
Size: 2428 496 2897
Fragments arranged by size:
2897 2428 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2060 2240 1163
Size: 573 324 180 4744
Fragments arranged by size:
4744 573 324 180
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2824 3271 4032 5060 1540
Size: 275 186 823 447 761 1028 2301
Fragments arranged by size:
2301 1028 823 761 447 275 186
BsiHKAI G_wGCw'C
Cuts at: 2166 2379 2484 3882 3967 5038 2166
Size: 213 105 1398 85 1071 2949
Fragments arranged by size:
2949 1398 1071 213 105 85
BsmI GAATG_Cn'
Cuts at: 3332 3425 3332
Size: 93 5728
BsmBI CGTCTCn'nnnn_
Cuts at: 2243 2243
Size: 5821
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2115 2166 2379 2469 2484 2751 2942
Size: 399 325 51 213 90 15 267 191
Cuts at: 2942 3140 3882 3967 5038 1391
Size: 198 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 325 267 213 198
191 90 85 51 15
BspEI T'CCGG_A
Cuts at: 5484 5484
Size: 5821
BspHI T'CATG_A
Cuts at: 3710 5440 3710
Size: 1730 4091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2217 2406 3176 844
Size: 1373 189 770 3489
Fragments arranged by size:
3489 1373 770 189
BsrBI CCG'CTC
Cuts at: 1394 1823 3708 1394
Size: 429 1885 3507
Fragments arranged by size:
3507 1885 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4316 4490 65
Size: 1183 3068 174 1396
Fragments arranged by size:
3068 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2861 4465 4652 1700
Size: 110 1051 1604 187 2869
Fragments arranged by size:
2869 1604 1051 187 110
BsrGI T'GTAC_A
Cuts at: 96 2181 96
Size: 2085 3736
BssHII G'CGCG_C
Cuts at: 1925 2149 2151 2660 1925
Size: 224 2 509 5086
Fragments arranged by size:
5086 509 224 2
BssSI C'ACGA_G
Cuts at: 2576 3881 4893 2576
Size: 1305 1012 3504
Fragments arranged by size:
3504 1305 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2383 794
Size: 1589 4232
BstBI TT'CG_AA
Cuts at: 4625 4625
Size: 5821
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5821
BstYI r'GATC_y
Cuts at: 1972 3151 3518 3903 3920 5372 5458 5470 5816
Size: 1179 367 385 17 1452 86 12 346
Cuts at: 5816 1972
Size: 1977
Fragments arranged by size:
1977 1452 1179 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5269
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5082
Fragments arranged by size:
5082 683 56
BtsI GCAGTG_nn'
Cuts at: 2252 3337 4131 4151 2252
Size: 1085 794 20 3922
Fragments arranged by size:
3922 1085 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5821
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5786
DraI TTT'AAA
Cuts at: 3125 3125
Size: 5821
DraIII CAC_nnn'GTG
Cuts at: 2083 2170 3099 2083
Size: 87 929 4805
Fragments arranged by size:
4805 929 87
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4828 809
Size: 507 3512 1802
Fragments arranged by size:
3512 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2199 2586 2802
Size: 54 1425 50 275 18 369 387 216
Cuts at: 2802 2859 2964 3268 4157 5786 8
Size: 57 105 304 889 1629 43
Fragments arranged by size:
1629 1425 889 387 369 304 275 216
105 57 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3268 1537
Size: 275 1456 4090
Fragments arranged by size:
4090 1456 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2219 3751 1353
Size: 866 1532 3423
Fragments arranged by size:
3423 1532 866
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2707 2998 3103 4408 4782 4928
Size: 1064 138 1118 291 105 1305 374 146
Cuts at: 4928 387
Size: 1280
Fragments arranged by size:
1305 1280 1118 1064 374 291 146 138
105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2524 3884 4481 5268 5536 1239
Size: 393 892 1360 597 787 268 1524
Fragments arranged by size:
1524 1360 892 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2487 2509 2958 2487
Size: 22 449 5350
Fragments arranged by size:
5350 449 22
EcoRI G'AATT_C
Cuts at: 3130 3508 3130
Size: 378 5443
FspI TGC'GCA
Cuts at: 4327 4327
Size: 5821
HaeII r_GCGC'y
Cuts at: 1678 2301 2447 2597 2868 2907 2990 4968 1678
Size: 623 146 150 271 39 83 1978 2531
Fragments arranged by size:
2531 1978 623 271 150 146 83 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3062 3094 4462 4494 4536
Size: 32 135 32 1490 32 1368 32 42
Cuts at: 4536 4568 1373
Size: 32 2626
Fragments arranged by size:
2626 1490 1368 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3165 3411 669
Size: 988 281 1227 246 3079
Fragments arranged by size:
3079 1227 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5821
HpaI GTT'AAC
Cuts at: 3411 3411
Size: 5821
KasI G'GCGC_C
Cuts at: 2297 2864 2297
Size: 567 5254
KpnI G_GTAC'C
Cuts at: 3146 3146
Size: 5821
MfeI C'AATT_G
Cuts at: 3420 3420
Size: 5821
MluI A'CGCG_T
Cuts at: 5697 5697
Size: 5821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3085 3093 4935 5119 1305
Size: 1780 8 1842 184 2007
Fragments arranged by size:
2007 1842 1780 184 8
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3999
Fragments arranged by size:
3999 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2351 2453 3779 4138 4297 518
Size: 648 1185 102 1326 359 159 2042
Fragments arranged by size:
2042 1326 1185 648 359 159 102
MspA1I CmG'CkG
Cuts at: 1606 2328 2383 2686 2887 3237 3914 5062 5307
Size: 722 55 303 201 350 677 1148 245
Cuts at: 5307 1606
Size: 2120
Fragments arranged by size:
2120 1148 722 677 350 303 245 201
55
NaeI GCC'GGC
Cuts at: 2863 2863
Size: 5821
NarI GG'CG_CC
Cuts at: 2298 2865 2298
Size: 567 5254
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5269
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5821
NgoMIV G'CCGG_C
Cuts at: 2861 2861
Size: 5821
NheI G'CTAG_C
Cuts at: 1052 2128 1052
Size: 1076 4745
NotI GC'GGCC_GC
Cuts at: 3268 3268
Size: 5821
NspI r_CATG'y
Cuts at: 1132 1229 2188 2320 3179 4724 1132
Size: 97 959 132 859 1545 2229
Fragments arranged by size:
2229 1545 959 859 132 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5821
PciI A'CATG_T
Cuts at: 2184 2316 4720 2184
Size: 132 2404 3285
Fragments arranged by size:
3285 2404 132
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5821
PmeI GTTT'AAAC
Cuts at: 3125 3125
Size: 5821
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3890 3922 5403 5435 3890
Size: 32 1481 32 4276
Fragments arranged by size:
4276 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2509 2958 2509
Size: 449 5372
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5821
PsiI TTA'TAA
Cuts at: 3391 3391
Size: 5821
PspXI vC'TCGA_Gb
Cuts at: 2513 2513
Size: 5821
PstI C_TGCA'G
Cuts at: 830 3173 830
Size: 2343 3478
PvuI CG_AT'CG
Cuts at: 2001 2824 2001
Size: 823 4998
PvuII CAG'CTG
Cuts at: 2328 2328
Size: 5821
SalI G'TCGA_C
Cuts at: 1936 3163 1936
Size: 1227 4594
SapI GCTCTTCn'nnn_
Cuts at: 2219 2219
Size: 5821
SbfI CC_TGCA'GG
Cuts at: 3173 3173
Size: 5821
ScaI AGT'ACT
Cuts at: 4069 5781 4069
Size: 1712 4109
SfcI C'TryA_G
Cuts at: 826 1045 2089 3169 4304 4985 5176 826
Size: 219 1044 1080 1135 681 191 1471
Fragments arranged by size:
1471 1135 1080 1044 681 219 191
SfoI GGC'GCC
Cuts at: 2299 2866 2299
Size: 567 5254
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5821
SmaI CCC'GGG
Cuts at: 1872 3148 1872
Size: 1276 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2513 3925 4826 5088 820
Size: 197 926 570 1412 901 262 1553
Fragments arranged by size:
1553 1412 926 901 570 262 197
SnaBI TAC'GTA
Cuts at: 493 3612 493
Size: 3119 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5821
SphI G_CATG'C
Cuts at: 1132 3179 1132
Size: 2047 3774
SspI AAT'ATT
Cuts at: 5 52 3745 5
Size: 47 3693 2081
Fragments arranged by size:
3693 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5821
StyI C'CwwG_G
Cuts at: 513 1065 3260 513
Size: 552 2195 3074
Fragments arranged by size:
3074 2195 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3848 3865 4018 4203 4648 325
Size: 1255 2268 17 153 185 445 1498
Fragments arranged by size:
2268 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2892 4067
Size: 274 80 33 51 1443 204 711 1175
Cuts at: 4067 5779 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1175 711 274 204 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3085 3117 5781 5813 3085
Size: 32 2664 32 3093
Fragments arranged by size:
3093 2664 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5821
XbaI T'CTAG_A
Cuts at: 3157 3157
Size: 5821
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2875 2875
Size: 5821
XhoI C'TCGA_G
Cuts at: 1943 2513 1943
Size: 570 5251
XmaI C'CCGG_G
Cuts at: 1870 3146 1870
Size: 1276 4545
XmnI GAAnn'nnTTC
Cuts at: 3950 3950
Size: 5821
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3629 276
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PmlI
PspOMI PsrI RsrII SacI SacII SanDI SexAI SfiI
SrfI SwaI