Restriction map for
pFN21AE3755
(Circular) (Six-base) MAPSORT of: pFN21AE3755.seq Check: 8942 from: 1 to: 5788
pFN21AE3755 5788 bp
With 182 enzymes: SgfI *
March 28, 2012 12:04 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3598 278
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2493 3131 1937
Size: 556 638 4594
Fragments arranged by size:
4594 638 556
Acc65I G'GTAC_C
Cuts at: 3109 3109
Size: 5788
AclI AA'CG_TT
Cuts at: 1563 3915 4288 1563
Size: 2352 373 3063
Fragments arranged by size:
3063 2352 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2598 3851 5235 5503 1632
Size: 966 1253 1384 268 1917
Fragments arranged by size:
1917 1384 1253 966 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5591
AflIII A'CryG_T
Cuts at: 1203 4687 5664 1203
Size: 3484 977 1327
Fragments arranged by size:
3484 1327 977
AgeI A'CCGG_T
Cuts at: 4619 4619
Size: 5788
AhdI GACnn_n'nnGTC
Cuts at: 4517 4517
Size: 5788
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5788
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5788
AlwNI CAG_nnn'CTG
Cuts at: 2377 3531 5103 2377
Size: 1154 1572 3062
Fragments arranged by size:
3062 1572 1154
ApaLI G'TGCA_C
Cuts at: 3845 5001 3845
Size: 1156 4632
ApoI r'AATT_y
Cuts at: 1493 2138 3097 3323 3475 5584 1493
Size: 645 959 226 152 2109 1697
Fragments arranged by size:
2109 1697 959 645 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4342 160
Size: 872 3310 1606
Fragments arranged by size:
3310 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
AvaI C'yCGr_G
Cuts at: 1870 1943 3113 1870
Size: 73 1170 4545
Fragments arranged by size:
4545 1170 73
BamHI G'GATC_C
Cuts at: 2559 3118 3485 2559
Size: 559 367 4862
Fragments arranged by size:
4862 559 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2762 3109 4564 618
Size: 325 217 627 975 347 1455 1842
Fragments arranged by size:
1842 1455 975 627 347 325 217
BanII G_rGCy'C
Cuts at: 1391 3107 1391
Size: 1716 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2037 2946 2981 928
Size: 971 138 909 35 3735
Fragments arranged by size:
3735 971 909 138 35
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3979 4013 1903
Size: 34 2042 34 3678
Fragments arranged by size:
3678 2042 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3680 4890 3680
Size: 1210 4578
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5788
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4399 136
Size: 107 122 71 631 605 2727 1525
Fragments arranged by size:
2727 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2233 5783 2233
Size: 3550 2238
BlpI GC'TnA_GC
Cuts at: 3205 3205
Size: 5788
Bme1580I G_kGCm'C
Cuts at: 1790 2765 3849 5005 1790
Size: 975 1084 1156 2573
Fragments arranged by size:
2573 1156 1084 975
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5788
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2286 2454 2999
Size: 416 53 83 399 24 869 168 545
Cuts at: 2999 3519 4477 442
Size: 520 958 1753
Fragments arranged by size:
1753 958 869 545 520 416 399 168
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5788
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3057 3057
Size: 5788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2477 2717 4448 1239
Size: 1238 240 1731 2579
Fragments arranged by size:
2579 1731 1238 240
Bpu10I CC'TnA_GC
Cuts at: 3032 3032
Size: 5788
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3913 4778 5076 3913
Size: 865 298 4625
Fragments arranged by size:
4625 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4451 882
Size: 617 2952 2219
Fragments arranged by size:
2952 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3579 493
Size: 711 2375 2702
Fragments arranged by size:
2702 2375 711
BsaBI GATnn'nnATC
Cuts at: 5782 5782
Size: 5788
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3595 3977 4612 275
Size: 53 83 186 936 2062 382 635 1451
Fragments arranged by size:
2062 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2076 2467 4221 4619 4893 5040 5451 2076
Size: 391 1754 398 274 147 411 2413
Fragments arranged by size:
2413 1754 411 398 391 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5563
Fragments arranged by size:
5563 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2646 1186
Size: 1460 4328
BseYI C'CCAG_C
Cuts at: 4991 5487 4991
Size: 496 5292
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2139 2531 1163
Size: 573 403 392 4420
Fragments arranged by size:
4420 573 403 392
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2116 3238 3999 5027 1540
Size: 275 186 115 1122 761 1028 2301
Fragments arranged by size:
2301 1122 1028 761 275 186 115
BsiHKAI G_wGCw'C
Cuts at: 3849 3934 5005 3849
Size: 85 1071 4632
Fragments arranged by size:
4632 1071 85
BsmI GAATG_Cn'
Cuts at: 3299 3392 3299
Size: 93 5695
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2765 3107 3849 3934 5005 1391
Size: 399 975 342 742 85 1071 2174
Fragments arranged by size:
2174 1071 975 742 399 342 85
BspEI T'CCGG_A
Cuts at: 2076 2467 5451 2076
Size: 391 2984 2413
Fragments arranged by size:
2984 2413 391
BspHI T'CATG_A
Cuts at: 3677 5407 3677
Size: 1730 4058
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3143 844
Size: 2299 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3675 1394
Size: 429 1852 3507
Fragments arranged by size:
3507 1852 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4283 4457 65
Size: 1183 3035 174 1396
Fragments arranged by size:
3035 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4432 4619 1700
Size: 110 2622 187 2869
Fragments arranged by size:
2869 2622 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5788
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5788
BssSI C'ACGA_G
Cuts at: 3848 4860 3848
Size: 1012 4776
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5788
BstBI TT'CG_AA
Cuts at: 4592 4592
Size: 5788
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5788
BstYI r'GATC_y
Cuts at: 1972 2233 2559 3049 3118 3485 3870 3887 5339
Size: 261 326 490 69 367 385 17 1452
Cuts at: 5339 5425 5437 5783 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 490 385 367 346 326 261
86 69 17 12
BstZ17I GTA'TAC
Cuts at: 2494 2494
Size: 5788
BtgI C'CryG_G
Cuts at: 513 1065 2004 2292 2329 513
Size: 552 939 288 37 3972
Fragments arranged by size:
3972 939 552 288 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2734 487
Size: 683 56 1508 3541
Fragments arranged by size:
3541 1508 683 56
BtsI GCAGTG_nn'
Cuts at: 2275 3304 4098 4118 2275
Size: 1029 794 20 3945
Fragments arranged by size:
3945 1029 794 20
ClaI AT'CG_AT
Cuts at: 1554 2112 1554
Size: 558 5230
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5753
DraI TTT'AAA
Cuts at: 2143 3092 2143
Size: 949 4839
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2891 4795 809
Size: 507 1575 1904 1802
Fragments arranged by size:
1904 1802 1575 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3235 4124 5753
Size: 54 1425 50 275 18 1405 889 1629
Cuts at: 5753 8
Size: 43
Fragments arranged by size:
1629 1425 1405 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3235 1537
Size: 275 1423 4090
Fragments arranged by size:
4090 1423 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2520 2574 2943 2964 3718 1353
Size: 1167 54 369 21 754 3423
Fragments arranged by size:
3423 1167 754 369 54 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4375 4749 4895 387
Size: 1064 138 2786 374 146 1280
Fragments arranged by size:
2786 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2477 2598 2717 3851 4448 5235 5503
Size: 393 845 121 119 1134 597 787 268
Cuts at: 5503 1239
Size: 1524
Fragments arranged by size:
1524 1134 845 787 597 393 268 121
119
EcoO109I rG'GnC_Cy
Cuts at: 2312 2609 3028 2312
Size: 297 419 5072
Fragments arranged by size:
5072 419 297
EcoRI G'AATT_C
Cuts at: 3097 3475 3097
Size: 378 5410
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2437 2437
Size: 5788
FspI TGC'GCA
Cuts at: 4294 4294
Size: 5788
HaeII r_GCGC'y
Cuts at: 1678 4935 1678
Size: 3257 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2767 2799 4429 4461 4503
Size: 32 135 32 1195 32 1630 32 42
Cuts at: 4503 4535 1373
Size: 32 2626
Fragments arranged by size:
2626 1630 1195 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2589 3132 3378 669
Size: 988 281 651 543 246 3079
Fragments arranged by size:
3079 988 651 543 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5788
HpaI GTT'AAC
Cuts at: 3378 3378
Size: 5788
KpnI G_GTAC'C
Cuts at: 3113 3113
Size: 5788
MfeI C'AATT_G
Cuts at: 3387 3387
Size: 5788
MluI A'CGCG_T
Cuts at: 5664 5664
Size: 5788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2885 2937 4902 5086 1305
Size: 1580 52 1965 184 2007
Fragments arranged by size:
2007 1965 1580 184 52
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3966
Fragments arranged by size:
3966 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2349 3746 4105 4264 518
Size: 648 1183 1397 359 159 2042
Fragments arranged by size:
2042 1397 1183 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2934 3204 3881 5029 5274 1606
Size: 1328 270 677 1148 245 2120
Fragments arranged by size:
2120 1328 1148 677 270 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2292 2329 513
Size: 552 939 288 37 3972
Fragments arranged by size:
3972 939 552 288 37
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5788
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5788
NotI GC'GGCC_GC
Cuts at: 3235 3235
Size: 5788
NspI r_CATG'y
Cuts at: 1132 1229 3146 4691 1132
Size: 97 1917 1545 2229
Fragments arranged by size:
2229 1917 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5788
PciI A'CATG_T
Cuts at: 4687 4687
Size: 5788
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2335 2740 1108
Size: 1227 405 4156
Fragments arranged by size:
4156 1227 405
PfoI T'CCnGG_A
Cuts at: 2733 2733
Size: 5788
PmeI GTTT'AAAC
Cuts at: 3092 3092
Size: 5788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3857 3889 5370 5402 3857
Size: 32 1481 32 4243
Fragments arranged by size:
4243 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2312 2609 3028 2312
Size: 297 419 5072
Fragments arranged by size:
5072 419 297
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5788
PsiI TTA'TAA
Cuts at: 2157 3358 2157
Size: 1201 4587
PstI C_TGCA'G
Cuts at: 830 2241 3140 830
Size: 1411 899 3478
Fragments arranged by size:
3478 1411 899
PvuI CG_AT'CG
Cuts at: 2001 2116 2001
Size: 115 5673
PvuII CAG'CTG
Cuts at: 2934 2934
Size: 5788
SalI G'TCGA_C
Cuts at: 1936 3130 1936
Size: 1194 4594
SbfI CC_TGCA'GG
Cuts at: 3140 3140
Size: 5788
ScaI AGT'ACT
Cuts at: 4036 5748 4036
Size: 1712 4076
SfcI C'TryA_G
Cuts at: 826 1045 2237 3136 4271 4952 5143 826
Size: 219 1192 899 1135 681 191 1471
Fragments arranged by size:
1471 1192 1135 899 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5788
SmaI CCC'GGG
Cuts at: 1872 3115 1872
Size: 1243 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3892 4793 5055 820
Size: 197 926 1949 901 262 1553
Fragments arranged by size:
1949 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3579 493
Size: 3086 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5788
SphI G_CATG'C
Cuts at: 1132 3146 1132
Size: 2014 3774
SspI AAT'ATT
Cuts at: 5 52 3712 5
Size: 47 3660 2081
Fragments arranged by size:
3660 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5788
StyI C'CwwG_G
Cuts at: 513 1065 2004 2292 2329 3227 513
Size: 552 939 288 37 898 3074
Fragments arranged by size:
3074 939 898 552 288 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3815 3832 3985 4170 4615 325
Size: 1255 2235 17 153 185 445 1498
Fragments arranged by size:
2235 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2529 4034 5746
Size: 274 80 33 51 1443 552 1505 1712
Cuts at: 5746 96
Size: 138
Fragments arranged by size:
1712 1505 1443 552 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2058 2090 5748 5780 2058
Size: 32 3658 32 2066
Fragments arranged by size:
3658 2066 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5788
XbaI T'CTAG_A
Cuts at: 3124 3124
Size: 5788
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5788
XmaI C'CCGG_G
Cuts at: 1870 3113 1870
Size: 1243 4545
XmnI GAAnn'nnTTC
Cuts at: 3917 3917
Size: 5788
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3596 276
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI