Restriction map for
pFN21AE3751
(Circular) (Six-base) MAPSORT of: pFN21AE3751.seq Check: 497 from: 1 to: 9112
pFN21AE3751 9112 bp
With 182 enzymes: SgfI *
March 28, 2012 12:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2491 2491
Size: 9112
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5573 6922 278
Size: 53 83 186 936 4037 1349 2468
Fragments arranged by size:
4037 2468 1349 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2280 3132 6455 1937
Size: 343 852 3323 4594
Fragments arranged by size:
4594 3323 852 343
Acc65I G'GTAC_C
Cuts at: 6433 6433
Size: 9112
AclI AA'CG_TT
Cuts at: 1563 7239 7612 1563
Size: 5676 373 3063
Fragments arranged by size:
5676 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2148 2265 2603 2654 3221 4679 4694 4911
Size: 516 117 338 51 567 1458 15 217
Cuts at: 4911 5613 7175 8559 8827 1632
Size: 702 1562 1384 268 1917
Fragments arranged by size:
1917 1562 1458 1384 702 567 516 338
268 217 117 51 15
AfeI AGC'GCT
Cuts at: 3678 3678
Size: 9112
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8915
AflIII A'CryG_T
Cuts at: 1203 5203 8011 8988 1203
Size: 4000 2808 977 1327
Fragments arranged by size:
4000 2808 1327 977
AgeI A'CCGG_T
Cuts at: 7943 7943
Size: 9112
AhdI GACnn_n'nnGTC
Cuts at: 7841 7841
Size: 9112
AleI CACnn'nnGTG
Cuts at: 1166 2390 2471 4370 5237 5854 1166
Size: 1224 81 1899 867 617 4424
Fragments arranged by size:
4424 1899 1224 867 617 81
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2426 2979 3912 4253 5921 1184
Size: 1242 553 933 341 1668 4375
Fragments arranged by size:
4375 1668 1242 933 553 341
AlwNI CAG_nnn'CTG
Cuts at: 2126 3004 3478 3982 4013 4768 5006 5113 5651
Size: 878 474 504 31 755 238 107 538
Cuts at: 5651 5860 6302 6855 8427 2126
Size: 209 442 553 1572 2811
Fragments arranged by size:
2811 1572 878 755 553 538 504 474
442 238 209 107 31
ApaI G_GGCC'C
Cuts at: 3220 3541 3907 4745 4876 5036 5378 5386 5758
Size: 321 366 838 131 160 342 8 372
Cuts at: 5758 5809 3220
Size: 51 6523
Fragments arranged by size:
6523 838 372 366 342 321 160 131
51 8
ApaLI G'TGCA_C
Cuts at: 7169 8325 7169
Size: 1156 7956
ApoI r'AATT_y
Cuts at: 1493 3011 3373 6421 6647 6799 8908 1493
Size: 1518 362 3048 226 152 2109 1697
Fragments arranged by size:
3048 2109 1697 1518 362 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3404 7666 160
Size: 872 2372 4262 1606
Fragments arranged by size:
4262 2372 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9112
AvaI C'yCGr_G
Cuts at: 1870 1943 2080 2114 2639 3919 4476 4737 4831
Size: 73 137 34 525 1280 557 261 94
Cuts at: 4831 5060 5361 6437 1870
Size: 229 301 1076 4545
Fragments arranged by size:
4545 1280 1076 557 525 301 261 229
137 94 73 34
BamHI G'GATC_C
Cuts at: 2068 2158 3190 6442 6809 2068
Size: 90 1032 3252 367 4371
Fragments arranged by size:
4371 3252 1032 367 90
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2384 3295 5122 6433 7888
Size: 325 217 627 597 911 1827 1311 1455
Cuts at: 7888 618
Size: 1842
Fragments arranged by size:
1842 1827 1455 1311 911 627 597 325
217
BanII G_rGCy'C
Cuts at: 1391 2087 2541 3220 3541 3563 3907 4745 4876
Size: 696 454 679 321 22 344 838 131
Cuts at: 4876 5036 5378 5386 5565 5697 5758 5809 5944
Size: 160 342 8 179 132 61 51 135
Cuts at: 5944 6197 6295 6431 1391
Size: 253 98 136 4072
Fragments arranged by size:
4072 838 696 679 454 344 342 321
253 179 160 136 135 132 131 98
61 51 22 8
BbeI G_GCGC'C
Cuts at: 5126 5126
Size: 9112
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2253 4603 4972 6219 928
Size: 971 354 2350 369 1247 3821
Fragments arranged by size:
3821 2350 1247 971 369 354
BbvCI CC'TCA_GC
Cuts at: 2310 3797 4028 4082 5177 2310
Size: 1487 231 54 1095 6245
Fragments arranged by size:
6245 1487 1095 231 54
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3868 3902 7303 7337 1903
Size: 34 1931 34 3401 34 3678
Fragments arranged by size:
3678 3401 1931 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6325 7004 8214 6325
Size: 679 1210 7223
Fragments arranged by size:
7223 1210 679
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9112
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3831 3963 4049
Size: 107 122 71 631 605 2159 132 86
Cuts at: 4049 4358 4456 4730 5543 5701 6146 7723 136
Size: 309 98 274 813 158 445 1577 1525
Fragments arranged by size:
2159 1577 1525 813 631 605 445 309
274 158 132 122 107 98 86 71
BglII A'GATC_T
Cuts at: 2097 5043 9107 2097
Size: 2946 4064 2102
Fragments arranged by size:
4064 2946 2102
BlpI GC'TnA_GC
Cuts at: 6015 6529 6015
Size: 514 8598
Bme1580I G_kGCm'C
Cuts at: 1790 2436 3220 3541 3907 4278 4519 4745 4876
Size: 646 784 321 366 371 241 226 131
Cuts at: 4876 5000 5036 5378 5386 5758 5809 7173 8329
Size: 124 36 342 8 372 51 1364 1156
Cuts at: 8329 1790
Size: 2573
Fragments arranged by size:
2573 1364 1156 784 646 372 371 366
342 321 241 226 131 124 51 36
8
BmgBI CAC'GTC
Cuts at: 1318 6361 1318
Size: 5043 4069
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2572 2800 3223
Size: 416 53 83 399 24 1155 228 423
Cuts at: 3223 3310 3439 3466 6056 6843 7801 442
Size: 87 129 27 2590 787 958 1753
Fragments arranged by size:
2590 1753 1155 958 787 423 416 399
228 129 87 83 53 27 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9112
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4221 4221
Size: 9112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2656 2702 2717 2969 3888 4004 4216 4775
Size: 1417 46 15 252 919 116 212 559
Cuts at: 4775 5550 5735 5786 5996 6053 7772 1239
Size: 775 185 51 210 57 1719 2579
Fragments arranged by size:
2579 1719 1417 919 775 559 252 212
210 185 116 57 51 46 15
Bpu10I CC'TnA_GC
Cuts at: 2310 3797 4028 4037 4082 5177 5559 2310
Size: 1487 231 9 45 1095 382 5863
Fragments arranged by size:
5863 1487 1095 382 231 45 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4302 4495 4542 7237 8102 8400 4302
Size: 193 47 2695 865 298 5014
Fragments arranged by size:
5014 2695 865 298 193 47
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3521 4013 4291 4777 5075 5957 7775
Size: 617 2022 492 278 486 298 882 1818
Cuts at: 7775 882
Size: 2219
Fragments arranged by size:
2219 2022 1818 882 617 492 486 298
278
BsaAI yAC'GTr
Cuts at: 493 1204 2092 6903 493
Size: 711 888 4811 2702
Fragments arranged by size:
4811 2702 888 711
BsaBI GATnn'nnATC
Cuts at: 3330 9106 3330
Size: 5776 3336
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3997 4604 4637 5123
Size: 53 83 186 936 2464 607 33 486
Cuts at: 5123 5570 5666 6159 6919 7301 7936 275
Size: 447 96 493 760 382 635 1451
Fragments arranged by size:
2464 1451 936 760 635 607 493 486
447 382 186 96 83 53 33
BsaWI w'CCGG_w
Cuts at: 2844 3519 3552 3883 4212 5434 5948 7545 7943
Size: 675 33 331 329 1222 514 1597 398
Cuts at: 7943 8217 8364 8775 2844
Size: 274 147 411 3181
Fragments arranged by size:
3181 1597 1222 675 514 411 398 331
329 274 147 33
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3526 3556 5118 5148 5449
Size: 30 165 30 2123 30 1562 30 301
Cuts at: 5449 5468 5479 5498 1178
Size: 19 11 19 4792
Fragments arranged by size:
4792 2123 1562 301 165 30 30 30
30 19 19 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2068 2549 2852 2918 2921 2924 2984 3071
Size: 882 481 303 66 3 3 60 87
Cuts at: 3071 3074 3647 3771 3887 4765 5457 1186
Size: 3 573 124 116 878 692 4841
Fragments arranged by size:
4841 882 878 692 573 481 303 124
116 87 66 60 3 3 3
BseYI C'CCAG_C
Cuts at: 2372 2764 2873 2974 3775 4389 4761 4825 4919
Size: 392 109 101 801 614 372 64 94
Cuts at: 4919 5147 5386 5413 5461 5644 5784 5860 5920
Size: 228 239 27 48 183 140 76 60
Cuts at: 5920 8315 8811 2372
Size: 2395 496 2673
Fragments arranged by size:
2673 2395 801 614 496 392 372 239
228 183 140 109 101 94 76 64
60 48 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2853 2919 3107 3908 4048 4172 4193
Size: 573 1117 66 188 801 140 124 21
Cuts at: 4193 4264 4331 5950 6247 1163
Size: 71 67 1619 297 4028
Fragments arranged by size:
4028 1619 1117 801 573 297 188 140
124 71 67 66 21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4822 6023 6027 6562 7323 8351
Size: 275 186 2821 1201 4 535 761 1028
Cuts at: 8351 1540
Size: 2301
Fragments arranged by size:
2821 2301 1201 1028 761 535 275 186
4
BsiHKAI G_wGCw'C
Cuts at: 2541 2871 3563 3740 4038 4477 4526 4755 5244
Size: 330 692 177 298 439 49 229 489
Cuts at: 5244 5944 5982 6034 6366 7173 7258 8329 2541
Size: 700 38 52 332 807 85 1071 3324
Fragments arranged by size:
3324 1071 807 700 692 489 439 332
330 298 229 177 85 52 49 38
BsmI GAATG_Cn'
Cuts at: 6623 6716 6623
Size: 93 9019
BsmBI CGTCTCn'nnnn_
Cuts at: 3869 3869
Size: 9112
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2436 2541 2871 3220 3541 3563
Size: 399 297 349 105 330 349 321 22
Cuts at: 3563 3740 3907 4038 4278 4477 4519 4526 4745
Size: 177 167 131 240 199 42 7 219
Cuts at: 4745 4755 4876 5000 5036 5244 5378 5386 5565
Size: 10 121 124 36 208 134 8 179
Cuts at: 5565 5697 5758 5809 5944 5982 6034 6197 6295
Size: 132 61 51 135 38 52 163 98
Cuts at: 6295 6366 6431 7173 7258 8329 1391
Size: 71 65 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 349 349 330 321
297 240 219 208 199 179 177 167
163 135 134 132 131 124 121 105
98 85 71 65 61 52 51 42
38 36 22 10 8 7
BspEI T'CCGG_A
Cuts at: 2844 3519 8775 2844
Size: 675 5256 3181
Fragments arranged by size:
5256 3181 675
BspHI T'CATG_A
Cuts at: 2358 2439 3495 7001 8731 2358
Size: 81 1056 3506 1730 2739
Fragments arranged by size:
3506 2739 1730 1056 81
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2267 2328 2491 3880 6467 844
Size: 1423 61 163 1389 2587 3489
Fragments arranged by size:
3489 2587 1423 1389 163 61
BsrBI CCG'CTC
Cuts at: 1394 1823 2914 4858 6019 6999 1394
Size: 429 1091 1944 1161 980 3507
Fragments arranged by size:
3507 1944 1161 1091 980 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2215 7607 7781 65
Size: 1183 967 5392 174 1396
Fragments arranged by size:
5392 1396 1183 967 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4412 4552 4655 6022 7756 7943 1700
Size: 110 2602 140 103 1367 1734 187 2869
Fragments arranged by size:
2869 2602 1734 1367 187 140 110 103
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9112
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9112
BssSI C'ACGA_G
Cuts at: 2499 7172 8184 2499
Size: 4673 1012 3427
Fragments arranged by size:
4673 3427 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2614 3085 3094 4963 5113 794
Size: 1820 471 9 1869 150 4793
Fragments arranged by size:
4793 1869 1820 471 150 9
BstBI TT'CG_AA
Cuts at: 7916 7916
Size: 9112
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9112
BstXI CCAn_nnnn'nTGG
Cuts at: 2680 3187 3464 4615 5995 2680
Size: 507 277 1151 1380 5797
Fragments arranged by size:
5797 1380 1151 507 277
BstYI r'GATC_y
Cuts at: 1972 2068 2097 2158 2365 2727 2841 3190 5043
Size: 96 29 61 207 362 114 349 1853
Cuts at: 5043 6442 6809 7194 7211 8663 8749 8761 9107
Size: 1399 367 385 17 1452 86 12 346
Cuts at: 9107 1972
Size: 1977
Fragments arranged by size:
1977 1853 1452 1399 385 367 362 349
346 207 114 96 86 61 29 17
12
Bsu36I CC'TnA_GG
Cuts at: 4331 5118 5680 4331
Size: 787 562 7763
Fragments arranged by size:
7763 787 562
BtgI C'CryG_G
Cuts at: 513 1065 2004 3749 3858 3907 513
Size: 552 939 1745 109 49 5718
Fragments arranged by size:
5718 1745 939 552 109 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3324 487
Size: 683 56 2098 6275
Fragments arranged by size:
6275 2098 683 56
BtsI GCAGTG_nn'
Cuts at: 2138 2836 3592 3601 4160 5159 5518 5861 6032
Size: 698 756 9 559 999 359 343 171
Cuts at: 6032 6628 7422 7442 2138
Size: 596 794 20 3808
Fragments arranged by size:
3808 999 794 756 698 596 559 359
343 171 20 9
ClaI AT'CG_AT
Cuts at: 1554 4404 1554
Size: 2850 6262
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9077
DraI TTT'AAA
Cuts at: 3280 3378 6416 3280
Size: 98 3038 5976
Fragments arranged by size:
5976 3038 98
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5671 8119 809
Size: 507 4355 2448 1802
Fragments arranged by size:
4355 2448 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2941 4359 4819
Size: 54 1425 50 275 18 1111 1418 460
Cuts at: 4819 5038 5331 5544 5844 6020 6024 6138 6559
Size: 219 293 213 300 176 4 114 421
Cuts at: 6559 7448 9077 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1418 1111 889 460 421 300
293 275 219 213 176 114 54 50
43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 4819 6020 6024 6559 1537
Size: 275 3007 1201 4 535 4090
Fragments arranged by size:
4090 3007 1201 535 275 4
EarI CTCTTCn'nnn_
Cuts at: 1353 2748 3285 3378 4544 5895 7042 1353
Size: 1395 537 93 1166 1351 1147 3423
Fragments arranged by size:
3423 1395 1351 1166 1147 537 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3547 7699 8073 8219 387
Size: 1064 138 1958 4152 374 146 1280
Fragments arranged by size:
4152 1958 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2539 3561 5942 2539
Size: 1022 2381 5709
Fragments arranged by size:
5709 2381 1022
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2148 2265 2603 2654 2656 2702 2717
Size: 393 516 117 338 51 2 46 15
Cuts at: 2717 2969 3221 3888 4004 4216 4679 4694 4775
Size: 252 252 667 116 212 463 15 81
Cuts at: 4775 4911 5550 5613 5735 5786 5996 6053 7175
Size: 136 639 63 122 51 210 57 1122
Cuts at: 7175 7772 8559 8827 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1122 787 667 639 597 516 463
393 338 268 252 252 212 210 136
122 117 116 81 63 57 51 51
46 15 15 2
EcoNI CCTnn'n_nnAGG
Cuts at: 4257 4631 5117 4257
Size: 374 486 8252
Fragments arranged by size:
8252 486 374
EcoO109I rG'GnC_Cy
Cuts at: 3217 3537 3768 3782 3892 3903 3979 4444 4480
Size: 320 231 14 110 11 76 465 36
Cuts at: 4480 4741 4835 4872 4873 5033 5086 5110 5307
Size: 261 94 37 1 160 53 24 197
Cuts at: 5307 5374 5375 5382 5383 5673 5702 5754 5755
Size: 67 1 7 1 290 29 52 1
Cuts at: 5755 5805 5806 5971 3217
Size: 50 1 165 6358
Fragments arranged by size:
6358 465 320 290 261 231 197 165
160 110 94 76 67 53 52 50
37 36 29 24 14 11 7 1
1 1 1 1
EcoRI G'AATT_C
Cuts at: 6421 6799 6421
Size: 378 8734
FseI GG_CCGG'CC
Cuts at: 6026 6026
Size: 9112
FspI TGC'GCA
Cuts at: 7618 7618
Size: 9112
HaeII r_GCGC'y
Cuts at: 1678 2994 3680 4195 5126 8259 1678
Size: 1316 686 515 931 3133 2531
Fragments arranged by size:
3133 2531 1316 931 686 515
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2053 2060 2085 2092 3175
Size: 32 135 32 481 7 25 7 1083
Cuts at: 3175 3207 3526 3558 5344 5376 7753 7785 7827
Size: 32 319 32 1786 32 2377 32 42
Cuts at: 7827 7859 1373
Size: 32 2626
Fragments arranged by size:
2626 2377 1786 1083 481 319 135 42
32 32 32 32 32 32 32 25
7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 6456 6702 669
Size: 988 281 4518 246 3079
Fragments arranged by size:
4518 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3843 748
Size: 3095 6017
HpaI GTT'AAC
Cuts at: 6702 6702
Size: 9112
KasI G'GCGC_C
Cuts at: 5122 5122
Size: 9112
KpnI G_GTAC'C
Cuts at: 6437 6437
Size: 9112
MfeI C'AATT_G
Cuts at: 6711 6711
Size: 9112
MluI A'CGCG_T
Cuts at: 8988 8988
Size: 9112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4665 8226 8410 1305
Size: 3360 3561 184 2007
Fragments arranged by size:
3561 3360 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4361 5333 5546 6140 10
Size: 54 1425 343 2529 972 213 594 2982
Fragments arranged by size:
2982 2529 1425 972 594 343 213 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2390 2426 2471 4370 5237 5854 7070
Size: 648 1224 36 45 1899 867 617 1216
Cuts at: 7070 7429 7588 518
Size: 359 159 2042
Fragments arranged by size:
2042 1899 1224 1216 867 648 617 359
159 45 36
MspA1I CmG'CkG
Cuts at: 1606 2269 3082 3163 3576 3788 3860 4081 4189
Size: 663 813 81 413 212 72 221 108
Cuts at: 4189 4342 4393 4765 5093 5279 5872 6528 7205
Size: 153 51 372 328 186 593 656 677
Cuts at: 7205 8353 8598 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 813 677 663 656 593 413
372 328 245 221 212 186 153 108
81 72 51
NaeI GCC'GGC
Cuts at: 4414 4554 6024 4414
Size: 140 1470 7502
Fragments arranged by size:
7502 1470 140
NarI GG'CG_CC
Cuts at: 5123 5123
Size: 9112
NcoI C'CATG_G
Cuts at: 513 1065 2004 3749 513
Size: 552 939 1745 5876
Fragments arranged by size:
5876 1745 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9112
NgoMIV G'CCGG_C
Cuts at: 4412 4552 6022 4412
Size: 140 1470 7502
Fragments arranged by size:
7502 1470 140
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9112
NotI GC'GGCC_GC
Cuts at: 6559 6559
Size: 9112
NspI r_CATG'y
Cuts at: 1132 1229 3073 5207 6279 6380 6470 8015 1132
Size: 97 1844 2134 1072 101 90 1545 2229
Fragments arranged by size:
2229 2134 1844 1545 1072 101 97 90
PasI CC'CwG_GG
Cuts at: 1348 2515 4112 5029 5251 5371 5379 5697 1348
Size: 1167 1597 917 222 120 8 318 4763
Fragments arranged by size:
4763 1597 1167 917 318 222 120 8
PciI A'CATG_T
Cuts at: 5203 8011 5203
Size: 2808 6304
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4768 6302 1108
Size: 3660 1534 3918
Fragments arranged by size:
3918 3660 1534
PfoI T'CCnGG_A
Cuts at: 2295 2730 3285 3702 3865 5099 5612 6069 2295
Size: 435 555 417 163 1234 513 457 5338
Fragments arranged by size:
5338 1234 555 513 457 435 417 163
PmeI GTTT'AAAC
Cuts at: 6416 6416
Size: 9112
PmlI CAC'GTG
Cuts at: 2092 2092
Size: 9112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7181 7213 8694 8726 7181
Size: 32 1481 32 7567
Fragments arranged by size:
7567 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3892 4480 4835 5307 5673 3892
Size: 588 355 472 366 7331
Fragments arranged by size:
7331 588 472 366 355
PshAI GACnn'nnGTC
Cuts at: 1613 4603 5216 1613
Size: 2990 613 5509
Fragments arranged by size:
5509 2990 613
PsiI TTA'TAA
Cuts at: 6682 6682
Size: 9112
PspOMI G'GGCC_C
Cuts at: 3216 3537 3903 4741 4872 5032 5374 5382 5754
Size: 321 366 838 131 160 342 8 372
Cuts at: 5754 5805 3216
Size: 51 6523
Fragments arranged by size:
6523 838 372 366 342 321 160 131
51 8
PspXI vC'TCGA_Gb
Cuts at: 2639 2639
Size: 9112
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2264 2296 2264
Size: 32 9080
PstI C_TGCA'G
Cuts at: 830 2268 2610 2922 3009 4347 4503 5450 5500
Size: 1438 342 312 87 1338 156 947 50
Cuts at: 5500 6464 830
Size: 964 3478
Fragments arranged by size:
3478 1438 1338 964 947 342 312 156
87 50
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9112
PvuII CAG'CTG
Cuts at: 2269 3082 3163 4081 4342 4393 4765 5279 2269
Size: 813 81 918 261 51 372 514 6102
Fragments arranged by size:
6102 918 813 514 372 261 81 51
SacI G_AGCT'C
Cuts at: 2541 3563 5944 2541
Size: 1022 2381 5709
Fragments arranged by size:
5709 2381 1022
SacII CC_GC'GG
Cuts at: 3861 3861
Size: 9112
SalI G'TCGA_C
Cuts at: 1936 6454 1936
Size: 4518 4594
SanDI GG'GwC_CC
Cuts at: 4480 4835 5307 5673 4480
Size: 355 472 366 7919
Fragments arranged by size:
7919 472 366 355
SbfI CC_TGCA'GG
Cuts at: 5450 6464 5450
Size: 1014 8098
ScaI AGT'ACT
Cuts at: 2645 7360 9072 2645
Size: 4715 1712 2685
Fragments arranged by size:
4715 2685 1712
SfcI C'TryA_G
Cuts at: 826 1045 2264 2606 2918 3005 4343 4499 5446
Size: 219 1219 342 312 87 1338 156 947
Cuts at: 5446 5496 6460 7595 8276 8467 826
Size: 50 964 1135 681 191 1471
Fragments arranged by size:
1471 1338 1219 1135 964 947 681 342
312 219 191 156 87 50
SfiI GGCCn_nnn'nGGCC
Cuts at: 4358 5543 6146 4358
Size: 1185 603 7324
Fragments arranged by size:
7324 1185 603
SfoI GGC'GCC
Cuts at: 5124 5124
Size: 9112
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9112
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9112
SmaI CCC'GGG
Cuts at: 1872 4739 4833 6439 1872
Size: 2867 94 1606 4545
Fragments arranged by size:
4545 2867 1606 94
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2114 2639 4317 4510 4557 7216
Size: 197 926 171 525 1678 193 47 2659
Cuts at: 7216 8117 8379 820
Size: 901 262 1553
Fragments arranged by size:
2659 1678 1553 926 901 525 262 197
193 171 47
SnaBI TAC'GTA
Cuts at: 493 6903 493
Size: 6410 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9112
SphI G_CATG'C
Cuts at: 1132 3073 6380 6470 1132
Size: 1941 3307 90 3774
Fragments arranged by size:
3774 3307 1941 90
SspI AAT'ATT
Cuts at: 5 52 2809 7036 5
Size: 47 2757 4227 2081
Fragments arranged by size:
4227 2757 2081 47
StuI AGG'CCT
Cuts at: 1517 2573 2759 3935 1517
Size: 1056 186 1176 6694
Fragments arranged by size:
6694 1176 1056 186
StyI C'CwwG_G
Cuts at: 513 1065 2004 2226 2388 3749 3807 4253 4932
Size: 552 939 222 162 1361 58 446 679
Cuts at: 4932 5606 5657 6154 6551 513
Size: 674 51 497 397 3074
Fragments arranged by size:
3074 1361 939 679 674 552 497 446
397 222 162 58 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3413 3450 4190 4468 5227 7139 7156
Size: 1255 1833 37 740 278 759 1912 17
Cuts at: 7156 7309 7494 7939 325
Size: 153 185 445 1498
Fragments arranged by size:
1912 1833 1498 1255 759 740 445 278
185 153 37 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2643 3693 7358
Size: 274 80 33 51 1443 666 1050 3665
Cuts at: 7358 9070 96
Size: 1712 138
Fragments arranged by size:
3665 1712 1443 1050 666 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5118 5150 5151 5183 5449 5468 5481 5500 9072
Size: 32 1 32 266 19 13 19 3572
Cuts at: 9072 9104 5118
Size: 32 5126
Fragments arranged by size:
5126 3572 266 32 32 32 19 19
13 1
Tth111I GACn'n_nGTC
Cuts at: 1316 2462 4268 4681 1316
Size: 1146 1806 413 5747
Fragments arranged by size:
5747 1806 1146 413
XbaI T'CTAG_A
Cuts at: 6448 6448
Size: 9112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2323 5704 5995 2323
Size: 3381 291 5440
Fragments arranged by size:
5440 3381 291
XhoI C'TCGA_G
Cuts at: 1943 2114 2639 1943
Size: 171 525 8416
Fragments arranged by size:
8416 525 171
XmaI C'CCGG_G
Cuts at: 1870 4737 4831 6437 1870
Size: 2867 94 1606 4545
Fragments arranged by size:
4545 2867 1606 94
XmnI GAAnn'nnTTC
Cuts at: 7241 7241
Size: 9112
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5571 6920 276
Size: 53 83 186 936 4037 1349 2468
Fragments arranged by size:
4037 2468 1349 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII SacI SacII SalI SanDI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII
EcoRV FalI FspAI NruI NsiI PacI RsrII SapI
SexAI SrfI SwaI