Restriction map for
pFN21AE3723
(Circular) (Six-base) MAPSORT of: pFN21AE3723.seq Check: 4471 from: 1 to: 7039
pFN21AE3723 7039 bp
With 182 enzymes: SgfI *
July 6, 2012 17:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4849 278
Size: 53 83 186 936 3313 2468
Fragments arranged by size:
3313 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4382 1937
Size: 2445 4594
Acc65I G'GTAC_C
Cuts at: 4360 4360
Size: 7039
AclI AA'CG_TT
Cuts at: 1563 5166 5539 1563
Size: 3603 373 3063
Fragments arranged by size:
3603 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2575 3126 3211 3889 4352 5102 6486 6754
Size: 943 551 85 678 463 750 1384 268
Cuts at: 6754 1632
Size: 1917
Fragments arranged by size:
1917 1384 943 750 678 551 463 268
85
AfeI AGC'GCT
Cuts at: 2014 2014
Size: 7039
AflII C'TTAA_G
Cuts at: 820 1017 2846 820
Size: 197 1829 5013
Fragments arranged by size:
5013 1829 197
AflIII A'CryG_T
Cuts at: 1203 2820 5938 6915 1203
Size: 1617 3118 977 1327
Fragments arranged by size:
3118 1617 1327 977
AgeI A'CCGG_T
Cuts at: 5870 5870
Size: 7039
AhdI GACnn_n'nnGTC
Cuts at: 3325 5768 3325
Size: 2443 4596
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7039
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7039
AlwNI CAG_nnn'CTG
Cuts at: 4782 6354 4782
Size: 1572 5467
ApaLI G'TGCA_C
Cuts at: 3614 5096 6252 3614
Size: 1482 1156 4401
Fragments arranged by size:
4401 1482 1156
ApoI r'AATT_y
Cuts at: 1493 2468 2600 2852 3454 3829 4025 4348 4574
Size: 975 132 252 602 375 196 323 226
Cuts at: 4574 4726 6835 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 975 602 375 323 252 226
196 152 132
AseI AT'TA_AT
Cuts at: 160 1032 5593 160
Size: 872 4561 1606
Fragments arranged by size:
4561 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7039
AvaI C'yCGr_G
Cuts at: 1870 1943 2460 2690 3534 3765 4364 1870
Size: 73 517 230 844 231 599 4545
Fragments arranged by size:
4545 844 599 517 231 230 73
AvrII C'CTAG_G
Cuts at: 2710 2710
Size: 7039
BamHI G'GATC_C
Cuts at: 2741 4369 4736 2741
Size: 1628 367 5044
Fragments arranged by size:
5044 1628 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3358 3664 4283 4360 5815
Size: 325 217 627 1571 306 619 77 1455
Cuts at: 5815 618
Size: 1842
Fragments arranged by size:
1842 1571 1455 627 619 325 306 217
77
BanII G_rGCy'C
Cuts at: 1391 3101 3745 4085 4095 4358 1391
Size: 1710 644 340 10 263 4072
Fragments arranged by size:
4072 1710 644 340 263 10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3284 3401 4079 928
Size: 971 1385 117 678 3888
Fragments arranged by size:
3888 1385 971 678 117
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5230 5264 1903
Size: 34 3293 34 3678
Fragments arranged by size:
3678 3293 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4931 6141 4931
Size: 1210 5829
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7039
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3545 5650 136
Size: 107 122 71 631 605 1873 2105 1525
Fragments arranged by size:
2105 1873 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7034 7034
Size: 7039
BlpI GC'TnA_GC
Cuts at: 4456 4456
Size: 7039
Bme1580I G_kGCm'C
Cuts at: 1790 3618 4286 5100 6256 1790
Size: 1828 668 814 1156 2573
Fragments arranged by size:
2573 1828 1156 814 668
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7039
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2238 4770 5728
Size: 416 53 83 399 24 821 2532 958
Cuts at: 5728 442
Size: 1753
Fragments arranged by size:
2532 1753 958 821 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7039
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2738 2738
Size: 7039
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2263 2270 3404 5699 1239
Size: 1024 7 1134 2295 2579
Fragments arranged by size:
2579 2295 1134 1024 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2156 2753 5164 6029 6327 2156
Size: 597 2411 865 298 2868
Fragments arranged by size:
2868 2411 865 597 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5702 882
Size: 617 4203 2219
Fragments arranged by size:
4203 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2953 3988 4062 4830 493
Size: 711 1749 1035 74 768 2702
Fragments arranged by size:
2702 1749 1035 768 711 74
BsaBI GATnn'nnATC
Cuts at: 7033 7033
Size: 7039
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4846 5228 5863 275
Size: 53 83 186 936 3313 382 635 1451
Fragments arranged by size:
3313 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5472 5870 6144 6291 6702 5472
Size: 398 274 147 411 5809
Fragments arranged by size:
5809 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2063 2093 3083 3113 4295
Size: 30 165 30 660 30 990 30 1182
Cuts at: 4295 4325 1178
Size: 30 3892
Fragments arranged by size:
3892 1182 990 660 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2159 2266 3329 1186
Size: 973 107 1063 4896
Fragments arranged by size:
4896 1063 973 107
BseYI C'CCAG_C
Cuts at: 2087 2870 3674 3687 3797 3850 6242 6738 2087
Size: 783 804 13 110 53 2392 496 2388
Fragments arranged by size:
2392 2388 804 783 496 110 53 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2513 4232 1163
Size: 573 777 1719 3970
Fragments arranged by size:
3970 1719 777 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4489 5250 6278 1540
Size: 275 186 2488 761 1028 2301
Fragments arranged by size:
2488 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3101 3618 4085 5100 5185 6256 3101
Size: 517 467 1015 85 1071 3884
Fragments arranged by size:
3884 1071 1015 517 467 85
BsmI GAATG_Cn'
Cuts at: 4550 4643 4550
Size: 93 6946
BsmBI CGTCTCn'nnnn_
Cuts at: 3965 3965
Size: 7039
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3101 3618 3745 4085 4095 4286 4358
Size: 399 1311 517 127 340 10 191 72
Cuts at: 4358 5100 5185 6256 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1311 1071 742 517 399 340 191
127 85 72 10
BspEI T'CCGG_A
Cuts at: 6702 6702
Size: 7039
BspHI T'CATG_A
Cuts at: 2898 4928 6658 2898
Size: 2030 1730 3279
Fragments arranged by size:
3279 2030 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2553 2850 4394 844
Size: 1709 297 1544 3489
Fragments arranged by size:
3489 1709 1544 297
BsrBI CCG'CTC
Cuts at: 1394 1823 3472 4047 4926 1394
Size: 429 1649 575 879 3507
Fragments arranged by size:
3507 1649 879 575 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2602 4305 5534 5708 65
Size: 1183 1354 1703 1229 174 1396
Fragments arranged by size:
1703 1396 1354 1229 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2163 3879 5683 5870 1700
Size: 110 353 1716 1804 187 2869
Fragments arranged by size:
2869 1804 1716 353 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7039
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7039
BssSI C'ACGA_G
Cuts at: 4078 4199 5099 6111 4078
Size: 121 900 1012 5006
Fragments arranged by size:
5006 1012 900 121
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3832 794
Size: 3038 4001
BstBI TT'CG_AA
Cuts at: 5843 5843
Size: 7039
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7039
BstYI r'GATC_y
Cuts at: 1972 2741 3027 4113 4369 4736 5121 5138 6590
Size: 769 286 1086 256 367 385 17 1452
Cuts at: 6590 6676 6688 7034 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1086 769 385 367 346 286
256 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3312 3312
Size: 7039
BtgI C'CryG_G
Cuts at: 513 1065 2826 3006 3543 3570 3645 3668 4086
Size: 552 1761 180 537 27 75 23 418
Cuts at: 4086 4241 4290 513
Size: 155 49 3262
Fragments arranged by size:
3262 1761 552 537 418 180 155 75
49 27 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6300
Fragments arranged by size:
6300 683 56
BtsI GCAGTG_nn'
Cuts at: 3739 4003 4220 4555 5349 5369 3739
Size: 264 217 335 794 20 5409
Fragments arranged by size:
5409 794 335 264 217 20
ClaI AT'CG_AT
Cuts at: 1554 2908 1554
Size: 1354 5685
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7004
DraI TTT'AAA
Cuts at: 4343 4343
Size: 7039
DraIII CAC_nnn'GTG
Cuts at: 3991 3991
Size: 7039
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6046 809
Size: 507 4730 1802
Fragments arranged by size:
4730 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3069 3204 3484
Size: 54 1425 50 275 18 1239 135 280
Cuts at: 3484 3865 4486 5375 7004 8
Size: 381 621 889 1629 43
Fragments arranged by size:
1629 1425 1239 889 621 381 280 275
135 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4486 1537
Size: 275 2674 4090
Fragments arranged by size:
4090 2674 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2669 3018 4969 1353
Size: 1316 349 1951 3423
Fragments arranged by size:
3423 1951 1316 349
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2421 2462 3001 3349 3703 5626
Size: 1064 138 832 41 539 348 354 1923
Cuts at: 5626 6000 6146 387
Size: 374 146 1280
Fragments arranged by size:
1923 1280 1064 832 539 374 354 348
146 138 41
EcoICRI GAG'CTC
Cuts at: 3099 4083 3099
Size: 984 6055
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2263 2270 2575 3126 3211 3404 3889
Size: 393 631 7 305 551 85 193 485
Cuts at: 3889 4352 5102 5699 6486 6754 1239
Size: 463 750 597 787 268 1524
Fragments arranged by size:
1524 787 750 631 597 551 485 463
393 305 268 193 85 7
EcoNI CCTnn'n_nnAGG
Cuts at: 3485 3485
Size: 7039
EcoO109I rG'GnC_Cy
Cuts at: 2977 3531 3888 2977
Size: 554 357 6128
Fragments arranged by size:
6128 554 357
EcoRI G'AATT_C
Cuts at: 2600 3454 4348 4726 2600
Size: 854 894 378 4913
Fragments arranged by size:
4913 894 854 378
EcoRV GAT'ATC
Cuts at: 4278 4278
Size: 7039
FspI TGC'GCA
Cuts at: 5545 5545
Size: 7039
HaeII r_GCGC'y
Cuts at: 1678 2016 3168 3226 6186 1678
Size: 338 1152 58 2960 2531
Fragments arranged by size:
2960 2531 1152 338 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2751 2783 3311 3343 5680
Size: 32 135 32 1179 32 528 32 2337
Cuts at: 5680 5712 5754 5786 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2337 1179 528 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4383 4629 669
Size: 988 281 2445 246 3079
Fragments arranged by size:
3079 2445 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4334 748
Size: 3586 3453
HpaI GTT'AAC
Cuts at: 4629 4629
Size: 7039
KpnI G_GTAC'C
Cuts at: 4364 4364
Size: 7039
MfeI C'AATT_G
Cuts at: 4638 4638
Size: 7039
MluI A'CGCG_T
Cuts at: 6915 6915
Size: 7039
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2124 3832 6153 6337 1305
Size: 819 1708 2321 184 2007
Fragments arranged by size:
2321 2007 1708 819 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3071 3867 10
Size: 54 1425 343 1239 796 3182
Fragments arranged by size:
3182 1425 1239 796 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2931 4289 4997 5356 5515 518
Size: 648 1765 1358 708 359 159 2042
Fragments arranged by size:
2042 1765 1358 708 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2739 3146 3220 3572 3670 4140 4243 4455
Size: 1133 407 74 352 98 470 103 212
Cuts at: 4455 5132 6280 6525 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 1133 677 470 407 352 245
212 103 98 74
NaeI GCC'GGC
Cuts at: 3881 3881
Size: 7039
NcoI C'CATG_G
Cuts at: 513 1065 3006 4086 4290 513
Size: 552 1941 1080 204 3262
Fragments arranged by size:
3262 1941 1080 552 204
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7039
NgoMIV G'CCGG_C
Cuts at: 3879 3879
Size: 7039
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7039
NotI GC'GGCC_GC
Cuts at: 4486 4486
Size: 7039
NspI r_CATG'y
Cuts at: 1132 1229 2824 4171 4183 4397 5942 1132
Size: 97 1595 1347 12 214 1545 2229
Fragments arranged by size:
2229 1595 1545 1347 214 97 12
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7039
PciI A'CATG_T
Cuts at: 2820 5938 2820
Size: 3118 3921
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7039
PfoI T'CCnGG_A
Cuts at: 3030 3030
Size: 7039
PmeI GTTT'AAAC
Cuts at: 4343 4343
Size: 7039
PmlI CAC'GTG
Cuts at: 3988 4062 3988
Size: 74 6965
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5108 5140 6621 6653 5108
Size: 32 1481 32 5494
Fragments arranged by size:
5494 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2977 3531 2977
Size: 554 6485
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7039
PsiI TTA'TAA
Cuts at: 4609 4609
Size: 7039
PspXI vC'TCGA_Gb
Cuts at: 2460 2460
Size: 7039
PstI C_TGCA'G
Cuts at: 830 4391 830
Size: 3561 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7039
PvuII CAG'CTG
Cuts at: 3146 4140 3146
Size: 994 6045
SacI G_AGCT'C
Cuts at: 3101 4085 3101
Size: 984 6055
SacII CC_GC'GG
Cuts at: 3573 3671 4244 3573
Size: 98 573 6368
Fragments arranged by size:
6368 573 98
SalI G'TCGA_C
Cuts at: 1936 4381 1936
Size: 2445 4594
SanDI GG'GwC_CC
Cuts at: 3531 3531
Size: 7039
SbfI CC_TGCA'GG
Cuts at: 4391 4391
Size: 7039
ScaI AGT'ACT
Cuts at: 2498 5287 6999 2498
Size: 2789 1712 2538
Fragments arranged by size:
2789 2538 1712
SfcI C'TryA_G
Cuts at: 826 1045 4387 5522 6203 6394 826
Size: 219 3342 1135 681 191 1471
Fragments arranged by size:
3342 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7039
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7039
SmaI CCC'GGG
Cuts at: 1872 3536 3767 4366 1872
Size: 1664 231 599 4545
Fragments arranged by size:
4545 1664 599 231
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2171 2460 2768 2846 5143 6044
Size: 197 926 228 289 308 78 2297 901
Cuts at: 6044 6306 820
Size: 262 1553
Fragments arranged by size:
2297 1553 926 901 308 289 262 228
197 78
SnaBI TAC'GTA
Cuts at: 493 4830 493
Size: 4337 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7039
SphI G_CATG'C
Cuts at: 1132 4183 4397 1132
Size: 3051 214 3774
Fragments arranged by size:
3774 3051 214
SspI AAT'ATT
Cuts at: 5 52 4963 5
Size: 47 4911 2081
Fragments arranged by size:
4911 2081 47
StuI AGG'CCT
Cuts at: 1517 3491 3629 1517
Size: 1974 138 4927
Fragments arranged by size:
4927 1974 138
StyI C'CwwG_G
Cuts at: 513 1065 2064 2199 2710 3006 3354 4086 4290
Size: 552 999 135 511 296 348 732 204
Cuts at: 4290 4478 513
Size: 188 3074
Fragments arranged by size:
3074 999 732 552 511 348 296 204
188 135
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3765 3978 5066 5083 5236 5421 5866
Size: 1255 2185 213 1088 17 153 185 445
Cuts at: 5866 325
Size: 1498
Fragments arranged by size:
2185 1498 1255 1088 445 213 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2496 4164 5285
Size: 274 80 33 51 1443 519 1668 1121
Cuts at: 5285 6997 96
Size: 1712 138
Fragments arranged by size:
1712 1668 1443 1121 519 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2063 2095 2679 2711 3581 3613 6999 7031 2063
Size: 32 584 32 870 32 3386 32 2071
Fragments arranged by size:
3386 2071 870 584 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7039
XbaI T'CTAG_A
Cuts at: 4375 4375
Size: 7039
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2206 4084 2206
Size: 1878 5161
XhoI C'TCGA_G
Cuts at: 1943 2460 1943
Size: 517 6522
XmaI C'CCGG_G
Cuts at: 1870 3534 3765 4364 1870
Size: 1664 231 599 4545
Fragments arranged by size:
4545 1664 599 231
XmnI GAAnn'nnTTC
Cuts at: 2294 4026 5168 2294
Size: 1732 1142 4165
Fragments arranged by size:
4165 1732 1142
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4847 276
Size: 53 83 186 936 3313 2468
Fragments arranged by size:
3313 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI PvuII SacI SacII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstXI BstZ17I FalI FseI FspAI KasI
NarI NruI NsiI PacI PspOMI PsrI RsrII SapI
SexAI SfiI SfoI SrfI SwaI