Restriction map for
pFN21AE3721
(Circular) (Six-base) MAPSORT of: pFN21AE3721.seq Check: 6629 from: 1 to: 5944
pFN21AE3721 5944 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3754 278
Size: 53 83 186 936 2218 2468
Fragments arranged by size:
2468 2218 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2271 3287 1937
Size: 334 1016 4594
Fragments arranged by size:
4594 1016 334
Acc65I G'GTAC_C
Cuts at: 3265 3265
Size: 5944
AclI AA'CG_TT
Cuts at: 1563 4071 4444 1563
Size: 2508 373 3063
Fragments arranged by size:
3063 2508 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2281 4007 5391 5659 1632
Size: 649 1726 1384 268 1917
Fragments arranged by size:
1917 1726 1384 649 268
AfeI AGC'GCT
Cuts at: 2378 2378
Size: 5944
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5747
AflIII A'CryG_T
Cuts at: 1203 2829 4843 5820 1203
Size: 1626 2014 977 1327
Fragments arranged by size:
2014 1626 1327 977
AgeI A'CCGG_T
Cuts at: 4775 4775
Size: 5944
AhdI GACnn_n'nnGTC
Cuts at: 4673 4673
Size: 5944
AleI CACnn'nnGTG
Cuts at: 1166 2453 1166
Size: 1287 4657
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5944
AlwNI CAG_nnn'CTG
Cuts at: 2611 2717 3687 5259 2611
Size: 106 970 1572 3296
Fragments arranged by size:
3296 1572 970 106
ApaLI G'TGCA_C
Cuts at: 4001 5157 4001
Size: 1156 4788
ApoI r'AATT_y
Cuts at: 1493 3253 3479 3631 5740 1493
Size: 1760 226 152 2109 1697
Fragments arranged by size:
2109 1760 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2571 4498 160
Size: 872 1539 1927 1606
Fragments arranged by size:
1927 1606 1539 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5944
AvaI C'yCGr_G
Cuts at: 1870 1943 3269 1870
Size: 73 1326 4545
Fragments arranged by size:
4545 1326 73
BamHI G'GATC_C
Cuts at: 3274 3641 3274
Size: 367 5577
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2253 3265 4720 618
Size: 325 217 627 466 1012 1455 1842
Fragments arranged by size:
1842 1455 1012 627 466 325 217
BanII G_rGCy'C
Cuts at: 1391 3263 1391
Size: 1872 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4973
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4135 4169 1903
Size: 34 2198 34 3678
Fragments arranged by size:
3678 2198 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3836 5046 3836
Size: 1210 4734
BclI T'GATC_A
Cuts at: 1548 2112 1548
Size: 564 5380
BfrBI ATG'CAT
Cuts at: 2725 2725
Size: 5944
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4555 136
Size: 107 122 71 631 605 2883 1525
Fragments arranged by size:
2883 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5939 5939
Size: 5944
BlpI GC'TnA_GC
Cuts at: 3361 3361
Size: 5944
Bme1580I G_kGCm'C
Cuts at: 1790 2256 4005 5161 1790
Size: 466 1749 1156 2573
Fragments arranged by size:
2573 1749 1156 466
BmgBI CAC'GTC
Cuts at: 1318 3001 1318
Size: 1683 4261
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2705 2767 3675
Size: 416 53 83 399 24 1288 62 908
Cuts at: 3675 4633 442
Size: 958 1753
Fragments arranged by size:
1753 1288 958 908 416 399 83 62
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5944
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 2108 2393 2817 4604 1239
Size: 836 33 285 424 1787 2579
Fragments arranged by size:
2579 1787 836 424 285 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4069 4934 5232 4069
Size: 865 298 4781
Fragments arranged by size:
4781 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4607 882
Size: 617 3108 2219
Fragments arranged by size:
3108 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3735 493
Size: 711 2531 2702
Fragments arranged by size:
2702 2531 711
BsaBI GATnn'nnATC
Cuts at: 2354 5938 2354
Size: 3584 2360
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3751 4133 4768 275
Size: 53 83 186 936 2218 382 635 1451
Fragments arranged by size:
2218 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2512 4377 4775 5049 5196 5607 2512
Size: 1865 398 274 147 411 2849
Fragments arranged by size:
2849 1865 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5719
Fragments arranged by size:
5719 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2152 1186
Size: 966 4978
BseYI C'CCAG_C
Cuts at: 5147 5643 5147
Size: 496 5448
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2519 2564 1163
Size: 573 783 45 4543
Fragments arranged by size:
4543 783 573 45
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2469 3394 4155 5183 1540
Size: 275 186 468 925 761 1028 2301
Fragments arranged by size:
2301 1028 925 761 468 275 186
BsiHKAI G_wGCw'C
Cuts at: 2217 4005 4090 5161 2217
Size: 1788 85 1071 3000
Fragments arranged by size:
3000 1788 1071 85
BsmI GAATG_Cn'
Cuts at: 2065 3455 3548 2065
Size: 1390 93 4461
Fragments arranged by size:
4461 1390 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2217 2256 3263 4005 4090 5161 1391
Size: 399 427 39 1007 742 85 1071 2174
Fragments arranged by size:
2174 1071 1007 742 427 399 85 39
BspEI T'CCGG_A
Cuts at: 5607 5607
Size: 5944
BspHI T'CATG_A
Cuts at: 3833 5563 3833
Size: 1730 4214
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3299 844
Size: 2455 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3831 1394
Size: 429 2008 3507
Fragments arranged by size:
3507 2008 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2790 4439 4613 65
Size: 1183 1542 1649 174 1396
Fragments arranged by size:
1649 1542 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3225 4588 4775 1700
Size: 110 1415 1363 187 2869
Fragments arranged by size:
2869 1415 1363 187 110
BsrGI T'GTAC_A
Cuts at: 96 2847 96
Size: 2751 3193
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5944
BssSI C'ACGA_G
Cuts at: 3025 4004 5016 3025
Size: 979 1012 3953
Fragments arranged by size:
3953 1012 979
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5944
BstBI TT'CG_AA
Cuts at: 4748 4748
Size: 5944
BstEII G'GTnAC_C
Cuts at: 1184 2025 1184
Size: 841 5103
BstXI CCAn_nnnn'nTGG
Cuts at: 2038 2249 2038
Size: 211 5733
BstYI r'GATC_y
Cuts at: 1972 2355 3274 3641 4026 4043 5495 5581 5593
Size: 383 919 367 385 17 1452 86 12
Cuts at: 5593 5939 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 919 385 383 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2490 2490
Size: 5944
BtgI C'CryG_G
Cuts at: 513 1065 2391 513
Size: 552 1326 4066
Fragments arranged by size:
4066 1326 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5205
Fragments arranged by size:
5205 683 56
BtsI GCAGTG_nn'
Cuts at: 2552 3460 4254 4274 2552
Size: 908 794 20 4222
Fragments arranged by size:
4222 908 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5944
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2815 2850 529
Size: 35 2251 35 3623
Fragments arranged by size:
3623 2251 35 35
DraI TTT'AAA
Cuts at: 3248 3248
Size: 5944
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4951 809
Size: 507 3635 1802
Fragments arranged by size:
3635 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2239 2854 3054
Size: 54 1425 50 275 18 409 615 200
Cuts at: 3054 3099 3188 3391 4280 5909 8
Size: 45 89 203 889 1629 43
Fragments arranged by size:
1629 1425 889 615 409 275 203 200
89 54 50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3391 1537
Size: 275 1579 4090
Fragments arranged by size:
4090 1579 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3038 3874 1353
Size: 1685 836 3423
Fragments arranged by size:
3423 1685 836
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4531 4905 5051 387
Size: 1064 138 2942 374 146 1280
Fragments arranged by size:
2942 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2108 2281 2393 2817 4007 4604
Size: 393 443 33 173 112 424 1190 597
Cuts at: 4604 5391 5659 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1190 787 597 443 424 393 268
173 112 33
EcoRI G'AATT_C
Cuts at: 3253 3631 3253
Size: 378 5566
FspI TGC'GCA
Cuts at: 4450 4450
Size: 5944
HaeII r_GCGC'y
Cuts at: 1678 2380 5091 1678
Size: 702 2711 2531
Fragments arranged by size:
2711 2531 702
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4585 4617 4659 4691 1373
Size: 32 135 32 3013 32 42 32 2626
Fragments arranged by size:
3013 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3288 3534 669
Size: 988 281 1350 246 3079
Fragments arranged by size:
3079 1350 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2744 3078 748
Size: 1996 334 3614
Fragments arranged by size:
3614 1996 334
HpaI GTT'AAC
Cuts at: 3534 3534
Size: 5944
KpnI G_GTAC'C
Cuts at: 3269 3269
Size: 5944
MfeI C'AATT_G
Cuts at: 3543 3543
Size: 5944
MluI A'CGCG_T
Cuts at: 5820 5820
Size: 5944
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2935 5058 5242 1305
Size: 1630 2123 184 2007
Fragments arranged by size:
2123 2007 1630 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3056 3101 3190 10
Size: 54 1425 343 1224 45 89 2764
Fragments arranged by size:
2764 1425 1224 343 89 54 45
MslI CAynn'nnrTG
Cuts at: 518 1166 2247 2453 2456 2864 3902 4261 4420
Size: 648 1081 206 3 408 1038 359 159
Cuts at: 4420 518
Size: 2042
Fragments arranged by size:
2042 1081 1038 648 408 359 206 159
3
MspA1I CmG'CkG
Cuts at: 1606 2179 2853 3360 4037 5185 5430 1606
Size: 573 674 507 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 674 573 507 245
NaeI GCC'GGC
Cuts at: 3227 3227
Size: 5944
NcoI C'CATG_G
Cuts at: 513 1065 2391 513
Size: 552 1326 4066
Fragments arranged by size:
4066 1326 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5944
NgoMIV G'CCGG_C
Cuts at: 3225 3225
Size: 5944
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5944
NotI GC'GGCC_GC
Cuts at: 3391 3391
Size: 5944
NsiI A_TGCA'T
Cuts at: 2727 2727
Size: 5944
NspI r_CATG'y
Cuts at: 1132 1229 2833 3302 4847 1132
Size: 97 1604 469 1545 2229
Fragments arranged by size:
2229 1604 1545 469 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5944
PciI A'CATG_T
Cuts at: 2829 4843 2829
Size: 2014 3930
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2397 2717 3108 1108
Size: 1289 320 391 3944
Fragments arranged by size:
3944 1289 391 320
PfoI T'CCnGG_A
Cuts at: 2184 2184
Size: 5944
PmeI GTTT'AAAC
Cuts at: 3248 3248
Size: 5944
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4013 4045 5526 5558 4013
Size: 32 1481 32 4399
Fragments arranged by size:
4399 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5944
PsiI TTA'TAA
Cuts at: 3514 3514
Size: 5944
PstI C_TGCA'G
Cuts at: 830 3296 830
Size: 2466 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5944
SalI G'TCGA_C
Cuts at: 1936 3286 1936
Size: 1350 4594
SbfI CC_TGCA'GG
Cuts at: 3296 3296
Size: 5944
ScaI AGT'ACT
Cuts at: 3131 4192 5904 3131
Size: 1061 1712 3171
Fragments arranged by size:
3171 1712 1061
SexAI A'CCwGG_T
Cuts at: 2716 2716
Size: 5944
SfcI C'TryA_G
Cuts at: 826 1045 3292 4427 5108 5299 826
Size: 219 2247 1135 681 191 1471
Fragments arranged by size:
2247 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5944
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5944
SmaI CCC'GGG
Cuts at: 1872 3271 1872
Size: 1399 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4048 4949 5211 820
Size: 197 926 2105 901 262 1553
Fragments arranged by size:
2105 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3735 493
Size: 3242 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5944
SphI G_CATG'C
Cuts at: 1132 3302 1132
Size: 2170 3774
SspI AAT'ATT
Cuts at: 5 52 3868 5
Size: 47 3816 2081
Fragments arranged by size:
3816 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5944
StyI C'CwwG_G
Cuts at: 513 1065 2391 3148 3383 513
Size: 552 1326 757 235 3074
Fragments arranged by size:
3074 1326 757 552 235
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2044 2455 3971 3988 4141 4326 4771
Size: 1255 464 411 1516 17 153 185 445
Cuts at: 4771 325
Size: 1498
Fragments arranged by size:
1516 1498 1255 464 445 411 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2847 3129 4190
Size: 274 80 33 51 1443 870 282 1061
Cuts at: 4190 5902 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1061 870 282 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5904 5936 5904
Size: 32 5912
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5944
XbaI T'CTAG_A
Cuts at: 3280 3280
Size: 5944
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2368 2011
Size: 357 5587
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5944
XmaI C'CCGG_G
Cuts at: 1870 3269 1870
Size: 1399 4545
XmnI GAAnn'nnTTC
Cuts at: 2747 4073 2747
Size: 1326 4618
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3752 276
Size: 53 83 186 936 2218 2468
Fragments arranged by size:
2468 2218 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI