Restriction map for
pFN21AE3720
(Circular) (Six-base) MAPSORT of: pFN21AE3720.seq Check: 8653 from: 1 to: 12178
pFN21AE3720 12178 bp
With 182 enzymes: SgfI *
November 30, 2011 11:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6437 8966 6437
Size: 2529 9649
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5811 8166 9988 278
Size: 53 83 186 936 4275 2355 1822 2468
Fragments arranged by size:
4275 2468 2355 1822 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 9521 1937
Size: 7584 4594
Acc65I G'GTAC_C
Cuts at: 5096 9499 5096
Size: 4403 7775
AclI AA'CG_TT
Cuts at: 1563 10305 10678 1563
Size: 8742 373 3063
Fragments arranged by size:
8742 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2780 4383 4551 5004 5591 5744 6027 7894
Size: 1148 1603 168 453 587 153 283 1867
Cuts at: 7894 8338 8431 8596 9317 10241 11625 11893 1632
Size: 444 93 165 721 924 1384 268 1917
Fragments arranged by size:
1917 1867 1603 1384 1148 924 721 587
453 444 283 268 168 165 153 93
AfeI AGC'GCT
Cuts at: 2066 3452 5546 5758 5978 2066
Size: 1386 2094 212 220 8266
Fragments arranged by size:
8266 2094 1386 220 212
AflII C'TTAA_G
Cuts at: 820 1017 6598 820
Size: 197 5581 6400
Fragments arranged by size:
6400 5581 197
AflIII A'CryG_T
Cuts at: 1203 4411 4440 5625 9048 11077 12054 1203
Size: 3208 29 1185 3423 2029 977 1327
Fragments arranged by size:
3423 3208 2029 1327 1185 977 29
AgeI A'CCGG_T
Cuts at: 11009 11009
Size: 12178
AhdI GACnn_n'nnGTC
Cuts at: 6148 10907 6148
Size: 4759 7419
AleI CACnn'nnGTG
Cuts at: 1166 2345 3592 4532 5750 1166
Size: 1179 1247 940 1218 7594
Fragments arranged by size:
7594 1247 1218 1179 940
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3861 4507 1184
Size: 2677 646 8855
Fragments arranged by size:
8855 2677 646
AlwNI CAG_nnn'CTG
Cuts at: 2089 4763 6766 8173 8197 9921 11493 2089
Size: 2674 2003 1407 24 1724 1572 2774
Fragments arranged by size:
2774 2674 2003 1724 1572 1407 24
ApaI G_GGCC'C
Cuts at: 2043 5154 8112 2043
Size: 3111 2958 6109
Fragments arranged by size:
6109 3111 2958
ApaLI G'TGCA_C
Cuts at: 2453 3783 7519 10235 11391 2453
Size: 1330 3736 2716 1156 3240
Fragments arranged by size:
3736 3240 2716 1330 1156
ApoI r'AATT_y
Cuts at: 1493 2333 2429 3653 5159 7231 7988 9487 9713
Size: 840 96 1224 1506 2072 757 1499 226
Cuts at: 9713 9865 11974 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 2072 1697 1506 1499 1224 840 757
226 152 96
AseI AT'TA_AT
Cuts at: 160 1032 3552 10732 160
Size: 872 2520 7180 1606
Fragments arranged by size:
7180 2520 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12178
AvaI C'yCGr_G
Cuts at: 1870 1943 2107 2141 2748 3599 8367 8774 8870
Size: 73 164 34 607 851 4768 407 96
Cuts at: 8870 9271 9503 1870
Size: 401 232 4545
Fragments arranged by size:
4768 4545 851 607 407 401 232 164
96 73 34
BamHI G'GATC_C
Cuts at: 4668 4816 5193 8769 9508 9875 4668
Size: 148 377 3576 739 367 6971
Fragments arranged by size:
6971 3576 739 377 367 148
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2518 2585 2888 3431 3947
Size: 325 217 627 731 67 303 543 516
Cuts at: 3947 5096 6011 7583 7635 9499 10954 618
Size: 1149 915 1572 52 1864 1455 1842
Fragments arranged by size:
1864 1842 1572 1455 1149 915 731 627
543 516 325 303 217 67 52
BanII G_rGCy'C
Cuts at: 1391 2030 2043 4016 4296 4523 4992 5154 5591
Size: 639 13 1973 280 227 469 162 437
Cuts at: 5591 6596 8112 9299 9425 9497 1391
Size: 1005 1516 1187 126 72 4072
Fragments arranged by size:
4072 1973 1516 1187 1005 639 469 437
280 227 162 126 72 13
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2276 3423 4070 7708 7900 9419 928
Size: 971 377 1147 647 3638 192 1519 3687
Fragments arranged by size:
3687 3638 1519 1147 971 647 377 192
BbvCI CC'TCA_GC
Cuts at: 8827 9200 8827
Size: 373 11805
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6121 6155 6411 6445 8597 8631 10369
Size: 34 4184 34 256 34 2152 34 1738
Cuts at: 10369 10403 1903
Size: 34 3678
Fragments arranged by size:
4184 3678 2152 1738 256 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4783 10070 11280 4783
Size: 5287 1210 5681
Fragments arranged by size:
5681 5287 1210
BclI T'GATC_A
Cuts at: 1548 2571 6831 1548
Size: 1023 4260 6895
Fragments arranged by size:
6895 4260 1023
BfrBI ATG'CAT
Cuts at: 4484 4484
Size: 12178
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5011 6245
Size: 107 122 71 631 605 337 3002 1234
Cuts at: 6245 6687 10789 136
Size: 442 4102 1525
Fragments arranged by size:
4102 3002 1525 1234 631 605 442 337
122 107 71
BglII A'GATC_T
Cuts at: 2321 12173 2321
Size: 9852 2326
BlpI GC'TnA_GC
Cuts at: 7255 8933 9595 7255
Size: 1678 662 9838
Fragments arranged by size:
9838 1678 662
Bme1580I G_kGCm'C
Cuts at: 1790 2043 2457 2891 3787 5154 6266 7119 7523
Size: 253 414 434 896 1367 1112 853 404
Cuts at: 7523 8112 10239 11395 1790
Size: 589 2127 1156 2573
Fragments arranged by size:
2573 2127 1367 1156 1112 896 853 589
434 414 404 253
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 12178
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4735 6184 6198
Size: 416 53 83 399 24 3318 1449 14
Cuts at: 6198 9299 9909 10867 442
Size: 3101 610 958 1753
Fragments arranged by size:
3318 3101 1753 1449 958 610 416 399
83 53 24 14
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 12178
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4196 4261 4196
Size: 65 12113
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4330 4349 4669 5021 5229 5656 6252 6436
Size: 3091 19 320 352 208 427 596 184
Cuts at: 6436 7671 8536 8592 10838 1239
Size: 1235 865 56 2246 2579
Fragments arranged by size:
3091 2579 2246 1235 865 596 427 352
320 208 184 56 19
Bpu10I CC'TnA_GC
Cuts at: 3268 5300 6815 7329 8827 9200 3268
Size: 2032 1515 514 1498 373 6246
Fragments arranged by size:
6246 2032 1515 1498 514 373
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3159 4976 10303 11168 11466 3159
Size: 1817 5327 865 298 3871
Fragments arranged by size:
5327 3871 1817 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2655 3222 5845 5897 7803 10841 882
Size: 617 1156 567 2623 52 1906 3038 2219
Fragments arranged by size:
3038 2623 2219 1906 1156 617 567 52
BsaAI yAC'GTr
Cuts at: 493 1204 2797 4419 4612 5626 6754 7666 8229
Size: 711 1593 1622 193 1014 1128 912 563
Cuts at: 8229 9969 493
Size: 1740 2702
Fragments arranged by size:
2702 1740 1622 1593 1128 1014 912 711
563 193
BsaBI GATnn'nnATC
Cuts at: 2562 4483 12172 2562
Size: 1921 7689 2568
Fragments arranged by size:
7689 2568 1921
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5808 7704 8163 9985
Size: 53 83 186 936 4275 1896 459 1822
Cuts at: 9985 10367 11002 275
Size: 382 635 1451
Fragments arranged by size:
4275 1896 1822 1451 936 635 459 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2611 4260 7848 10611 11009 11283 11430 11841 2611
Size: 1649 3588 2763 398 274 147 411 2948
Fragments arranged by size:
3588 2948 2763 1649 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4328 4358 7122 7152 1178
Size: 30 165 30 2925 30 2764 30 6204
Fragments arranged by size:
6204 2925 2764 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3413 4304 5833 7427 7578 7763 8020 9010
Size: 2227 891 1529 1594 151 185 257 990
Cuts at: 9010 9126 1186
Size: 116 4238
Fragments arranged by size:
4238 2227 1594 1529 990 891 257 185
151 116
BseYI C'CCAG_C
Cuts at: 6015 6289 8197 8358 9284 11381 11877 6015
Size: 274 1908 161 926 2097 496 6316
Fragments arranged by size:
6316 2097 1908 926 496 274 161
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2170 3349 3639 3868 4612 5489 6865
Size: 573 434 1179 290 229 744 877 1376
Cuts at: 6865 8326 8420 9387 1163
Size: 1461 94 967 3954
Fragments arranged by size:
3954 1461 1376 1179 967 877 744 573
434 290 229 94
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2023 6954 7180 7653 9628 10389
Size: 275 186 22 4931 226 473 1975 761
Cuts at: 10389 11417 1540
Size: 1028 2301
Fragments arranged by size:
4931 2301 1975 1028 761 473 275 226
186 22
BsiHKAI G_wGCw'C
Cuts at: 2457 3787 4992 5578 6210 6596 7475 7523 8570
Size: 1330 1205 586 632 386 879 48 1047
Cuts at: 8570 8934 9299 9425 10239 10324 11395 2457
Size: 364 365 126 814 85 1071 3240
Fragments arranged by size:
3240 1330 1205 1071 1047 879 814 632
586 386 365 364 126 85 48
BsmI GAATG_Cn'
Cuts at: 3266 4307 8315 9689 9782 3266
Size: 1041 4008 1374 93 5662
Fragments arranged by size:
5662 4008 1374 1041 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4197 4882 4197
Size: 685 11493
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2043 2457 2891 3787 4016 4296
Size: 399 240 13 414 434 896 229 280
Cuts at: 4296 4523 4992 5154 5578 5591 6210 6266 6596
Size: 227 469 162 424 13 619 56 330
Cuts at: 6596 7119 7475 7523 8112 8570 8934 9299 9425
Size: 523 356 48 589 458 364 365 126
Cuts at: 9425 9497 10239 10324 11395 1391
Size: 72 742 85 1071 2174
Fragments arranged by size:
2174 1071 896 742 619 589 523 469
458 434 424 414 399 365 364 356
330 280 240 229 227 162 126 85
72 56 48 13 13
BspEI T'CCGG_A
Cuts at: 2611 11841 2611
Size: 9230 2948
BspHI T'CATG_A
Cuts at: 10067 11797 10067
Size: 1730 10448
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3308 4960 5879 6437 8025 8320 8966 9533
Size: 2464 1652 919 558 1588 295 646 567
Cuts at: 9533 844
Size: 3489
Fragments arranged by size:
3489 2464 1652 1588 919 646 567 558
295
BsrBI CCG'CTC
Cuts at: 1394 1823 2878 2997 3045 10065 1394
Size: 429 1055 119 48 7020 3507
Fragments arranged by size:
7020 3507 1055 429 119 48
BsrDI GCAATG_nn'
Cuts at: 65 1248 2391 5137 9331 10673 10847 65
Size: 1183 1143 2746 4194 1342 174 1396
Fragments arranged by size:
4194 2746 1396 1342 1183 1143 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2018 4568 10822 11009 1700
Size: 110 208 2550 6254 187 2869
Fragments arranged by size:
6254 2869 2550 208 187 110
BsrGI T'GTAC_A
Cuts at: 96 6777 8291 96
Size: 6681 1514 3983
Fragments arranged by size:
6681 3983 1514
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 12178
BssSI C'ACGA_G
Cuts at: 3588 4663 7468 7814 8786 10238 11250 3588
Size: 1075 2805 346 972 1452 1012 4516
Fragments arranged by size:
4516 2805 1452 1075 1012 972 346
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2089 3617 7114 794
Size: 1295 1528 3497 5858
Fragments arranged by size:
5858 3497 1528 1295
BstBI TT'CG_AA
Cuts at: 7365 10982 7365
Size: 3617 8561
BstEII G'GTnAC_C
Cuts at: 1184 6556 1184
Size: 5372 6806
BstXI CCAn_nnnn'nTGG
Cuts at: 9433 9433
Size: 12178
BstYI r'GATC_y
Cuts at: 1972 2321 4668 4816 5193 5498 8526 8769 9123
Size: 349 2347 148 377 305 3028 243 354
Cuts at: 9123 9508 9875 10260 10277 11729 11815 11827 12173
Size: 385 367 385 17 1452 86 12 346
Cuts at: 12173 1972
Size: 1977
Fragments arranged by size:
3028 2347 1977 1452 385 385 377 367
354 349 346 305 243 148 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 6852 8391 6852
Size: 1539 10639
BtgI C'CryG_G
Cuts at: 513 1065 2004 4392 5487 6007 6474 9140 513
Size: 552 939 2388 1095 520 467 2666 3551
Fragments arranged by size:
3551 2666 2388 1095 939 552 520 467
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4653 6992 7273 487
Size: 683 56 3427 2339 281 5392
Fragments arranged by size:
5392 3427 2339 683 281 56
BtsI GCAGTG_nn'
Cuts at: 2475 2697 3618 3627 5424 9694 10488 10508 2475
Size: 222 921 9 1797 4270 794 20 4145
Fragments arranged by size:
4270 4145 1797 921 794 222 20 9
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 12178
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2191 2226 2563 2598 8283 8318 529
Size: 35 1627 35 337 35 5685 35 4389
Fragments arranged by size:
5685 4389 1627 337 35 35 35 35
DraI TTT'AAA
Cuts at: 2503 9482 2503
Size: 6979 5199
DraIII CAC_nnn'GTG
Cuts at: 3568 5884 7114 9307 3568
Size: 2316 1230 2193 6439
Fragments arranged by size:
6439 2316 2193 1230
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2783 5258 5339 6554 7565 8168 11185
Size: 507 1467 2475 81 1215 1011 603 3017
Cuts at: 11185 809
Size: 1802
Fragments arranged by size:
3017 2475 1802 1467 1215 1011 603 507
81
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2020 2740 6020
Size: 54 1425 50 275 18 190 720 3280
Cuts at: 6020 7177 9169 9625 10514 12143 8
Size: 1157 1992 456 889 1629 43
Fragments arranged by size:
3280 1992 1629 1425 1157 889 720 456
275 190 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2020 7177 9625 1537
Size: 275 208 5157 2448 4090
Fragments arranged by size:
5157 4090 2448 275 208
EarI CTCTTCn'nnn_
Cuts at: 1353 2535 2799 4230 4980 6524 6917 7733 8692
Size: 1182 264 1431 750 1544 393 816 959
Cuts at: 8692 8816 9078 9114 10108 1353
Size: 124 262 36 994 3423
Fragments arranged by size:
3423 1544 1431 1182 994 959 816 750
393 264 262 124 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3464 10765 11139 11285 387
Size: 1064 138 1875 7301 374 146 1280
Fragments arranged by size:
7301 1875 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4990 6594 9297 9423 4990
Size: 1604 2703 126 7745
Fragments arranged by size:
7745 2703 1604 126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2780 4330 4349 4383 4551 4669 5004
Size: 393 1148 1550 19 34 168 118 335
Cuts at: 5004 5021 5229 5591 5656 5744 6027 6252 6436
Size: 17 208 362 65 88 283 225 184
Cuts at: 6436 7671 7894 8338 8431 8536 8592 8596 9317
Size: 1235 223 444 93 105 56 4 721
Cuts at: 9317 10241 10838 11625 11893 1239
Size: 924 597 787 268 1524
Fragments arranged by size:
1550 1524 1235 1148 924 787 721 597
444 393 362 335 283 268 225 223
208 184 168 118 105 93 88 65
56 34 19 17 4
EcoO109I rG'GnC_Cy
Cuts at: 2039 5078 5150 5151 6199 6696 6763 6789 8043
Size: 3039 72 1 1048 497 67 26 1254
Cuts at: 8043 8108 8109 8170 9463 2039
Size: 65 1 61 1293 4754
Fragments arranged by size:
4754 3039 1293 1254 1048 497 72 67
65 61 26 1 1
EcoRI G'AATT_C
Cuts at: 2333 9487 9865 2333
Size: 7154 378 4646
Fragments arranged by size:
7154 4646 378
EcoRV GAT'ATC
Cuts at: 5176 5583 7174 8332 5176
Size: 407 1591 1158 9022
Fragments arranged by size:
9022 1591 1158 407
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2839 3000 3994 4388 2839
Size: 161 994 394 10629
Fragments arranged by size:
10629 994 394 161
FspI TGC'GCA
Cuts at: 2771 10684 2771
Size: 7913 4265
HaeII r_GCGC'y
Cuts at: 1678 2068 3454 5548 5760 5980 6474 8954 11325
Size: 390 1386 2094 212 220 494 2480 2371
Cuts at: 11325 1678
Size: 2531
Fragments arranged by size:
2531 2480 2371 2094 1386 494 390 220
212
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4003 4035 4505 4537 4657
Size: 32 135 32 2431 32 470 32 120
Cuts at: 4657 4689 4860 4892 5129 5161 5165 5197 6134
Size: 32 171 32 237 32 4 32 937
Cuts at: 6134 6166 6578 6610 6892 6924 7037 7069 10819
Size: 32 412 32 282 32 113 32 3750
Cuts at: 10819 10851 10893 10925 1373
Size: 32 42 32 2626
Fragments arranged by size:
3750 2626 2431 937 470 412 282 237
171 135 120 113 42 32 32 32
32 32 32 32 32 32 32 32
32 32 32 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3361 5332 5521 6411 6988 9522
Size: 988 281 1423 1971 189 890 577 2534
Cuts at: 9522 9768 669
Size: 246 3079
Fragments arranged by size:
3079 2534 1971 1423 988 890 577 281
246 189
HindIII A'AGCT_T
Cuts at: 748 2576 4094 5795 748
Size: 1828 1518 1701 7131
Fragments arranged by size:
7131 1828 1701 1518
HpaI GTT'AAC
Cuts at: 9768 9768
Size: 12178
KpnI G_GTAC'C
Cuts at: 5100 9503 5100
Size: 4403 7775
MfeI C'AATT_G
Cuts at: 9777 9777
Size: 12178
MluI A'CGCG_T
Cuts at: 12054 12054
Size: 12178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2853 2940 4144 4999 7959 8554 8597 8605
Size: 1548 87 1204 855 2960 595 43 8
Cuts at: 8605 9069 11292 11476 1305
Size: 464 2223 184 2007
Fragments arranged by size:
2960 2223 2007 1548 1204 855 595 464
184 87 43 8
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2742 10
Size: 54 1425 343 910 9446
Fragments arranged by size:
9446 1425 910 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2345 3592 3757 4439 4532 5750 7641
Size: 648 1179 1247 165 682 93 1218 1891
Cuts at: 7641 7942 9216 10136 10495 10654 518
Size: 301 1274 920 359 159 2042
Fragments arranged by size:
2042 1891 1274 1247 1218 1179 920 682
648 359 301 165 159 93
MspA1I CmG'CkG
Cuts at: 1606 2086 2677 3280 3845 4590 5041 5102 5489
Size: 480 591 603 565 745 451 61 387
Cuts at: 5489 6009 6019 6623 6772 6934 7136 8340 8358
Size: 520 10 604 149 162 202 1204 18
Cuts at: 8358 9130 9594 10271 11419 11664 1606
Size: 772 464 677 1148 245 2120
Fragments arranged by size:
2120 1204 1148 772 745 677 604 603
591 565 520 480 464 451 387 245
202 162 149 61 18 10
NaeI GCC'GGC
Cuts at: 2020 2020
Size: 12178
NcoI C'CATG_G
Cuts at: 513 1065 2004 4392 513
Size: 552 939 2388 8299
Fragments arranged by size:
8299 2388 939 552
NdeI CA'TA_TG
Cuts at: 387 8582 387
Size: 8195 3983
NgoMIV G'CCGG_C
Cuts at: 2018 2018
Size: 12178
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 12178
NotI GC'GGCC_GC
Cuts at: 9625 9625
Size: 12178
NruI TCG'CGA
Cuts at: 7491 7491
Size: 12178
NsiI A_TGCA'T
Cuts at: 4486 4486
Size: 12178
NspI r_CATG'y
Cuts at: 1132 1229 4415 6906 9536 11081 1132
Size: 97 3186 2491 2630 1545 2229
Fragments arranged by size:
3186 2630 2491 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2416 5731 8113 1348
Size: 1068 3315 2382 5413
Fragments arranged by size:
5413 3315 2382 1068
PciI A'CATG_T
Cuts at: 4411 11077 4411
Size: 6666 5512
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5705 8173 1108
Size: 4597 2468 5113
Fragments arranged by size:
5113 4597 2468
PfoI T'CCnGG_A
Cuts at: 2328 5398 2328
Size: 3070 9108
PmeI GTTT'AAAC
Cuts at: 9482 9482
Size: 12178
PmlI CAC'GTG
Cuts at: 4419 4419
Size: 12178
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3034 3066 10247 10279 11760 11792 3034
Size: 32 7181 32 1481 32 3420
Fragments arranged by size:
7181 3420 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 6199 6763 6789 8043 8170 9463 6199
Size: 564 26 1254 127 1293 8914
Fragments arranged by size:
8914 1293 1254 564 127 26
PshAI GACnn'nnGTC
Cuts at: 1613 6384 7379 1613
Size: 4771 995 6412
Fragments arranged by size:
6412 4771 995
PsiI TTA'TAA
Cuts at: 2196 9748 2196
Size: 7552 4626
PspOMI G'GGCC_C
Cuts at: 2039 5150 8108 2039
Size: 3111 2958 6109
Fragments arranged by size:
6109 3111 2958
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2971 3003 9025 9057 2971
Size: 32 6022 32 6092
Fragments arranged by size:
6092 6022 32 32
PstI C_TGCA'G
Cuts at: 830 2085 2482 3145 3576 6434 6771 7832 9530
Size: 1255 397 663 431 2858 337 1061 1698
Cuts at: 9530 830
Size: 3478
Fragments arranged by size:
3478 2858 1698 1255 1061 663 431 397
337
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12178
PvuII CAG'CTG
Cuts at: 2086 2677 3845 5041 6623 6772 8340 8358 9130
Size: 591 1168 1196 1582 149 1568 18 772
Cuts at: 9130 2086
Size: 5134
Fragments arranged by size:
5134 1582 1568 1196 1168 772 591 149
18
SacI G_AGCT'C
Cuts at: 4992 6596 9299 9425 4992
Size: 1604 2703 126 7745
Fragments arranged by size:
7745 2703 1604 126
SacII CC_GC'GG
Cuts at: 5490 6010 5490
Size: 520 11658
SalI G'TCGA_C
Cuts at: 1936 9520 1936
Size: 7584 4594
SanDI GG'GwC_CC
Cuts at: 6199 6789 6199
Size: 590 11588
SapI GCTCTTCn'nnn_
Cuts at: 4980 6524 7733 4980
Size: 1544 1209 9425
Fragments arranged by size:
9425 1544 1209
SbfI CC_TGCA'GG
Cuts at: 9530 9530
Size: 12178
ScaI AGT'ACT
Cuts at: 7246 8648 10426 12138 7246
Size: 1402 1778 1712 7286
Fragments arranged by size:
7286 1778 1712 1402
SexAI A'CCwGG_T
Cuts at: 6759 6759
Size: 12178
SfcI C'TryA_G
Cuts at: 826 1045 2081 2478 3141 3572 4129 6430 6767
Size: 219 1036 397 663 431 557 2301 337
Cuts at: 6767 7441 7504 7828 9031 9526 10661 11342 11533
Size: 674 63 324 1203 495 1135 681 191
Cuts at: 11533 826
Size: 1471
Fragments arranged by size:
2301 1471 1203 1135 1036 681 674 663
557 495 431 397 337 324 219 191
63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12178
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 12178
SmaI CCC'GGG
Cuts at: 1872 2109 2750 8369 8872 9505 1872
Size: 237 641 5619 503 633 4545
Fragments arranged by size:
5619 4545 641 633 503 237
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3174 4991 6598 10282 11183 11445
Size: 197 926 1231 1817 1607 3684 901 262
Cuts at: 11445 820
Size: 1553
Fragments arranged by size:
3684 1817 1607 1553 1231 926 901 262
197
SnaBI TAC'GTA
Cuts at: 493 9969 493
Size: 9476 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 12178
SphI G_CATG'C
Cuts at: 1132 9536 1132
Size: 8404 3774
SspI AAT'ATT
Cuts at: 5 52 3080 3556 7084 10102 5
Size: 47 3028 476 3528 3018 2081
Fragments arranged by size:
3528 3028 3018 2081 476 47
StuI AGG'CCT
Cuts at: 1517 6850 1517
Size: 5333 6845
StyI C'CwwG_G
Cuts at: 513 1065 2004 4372 4392 5318 5699 5832 7587
Size: 552 939 2368 20 926 381 133 1755
Cuts at: 7587 9617 513
Size: 2030 3074
Fragments arranged by size:
3074 2368 2030 1755 939 926 552 381
133 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2282 4328 5450 6029 6281 6968 7914
Size: 1255 702 2046 1122 579 252 687 946
Cuts at: 7914 8189 9318 10205 10222 10375 10560 11005 325
Size: 275 1129 887 17 153 185 445 1498
Fragments arranged by size:
2046 1498 1255 1129 1122 946 887 702
687 579 445 275 252 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2188 6489 6777
Size: 274 80 33 51 1443 211 4301 288
Cuts at: 6777 7244 8291 8646 10424 12136 96
Size: 467 1047 355 1778 1712 138
Fragments arranged by size:
4301 1778 1712 1443 1047 467 355 288
274 211 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5446 5478 6505 6537 7122 7154 12138 12170 5446
Size: 32 1027 32 585 32 4984 32 5454
Fragments arranged by size:
5454 4984 1027 585 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 6761 7703 8716 1316
Size: 5445 942 1013 4778
Fragments arranged by size:
5445 4778 1013 942
XbaI T'CTAG_A
Cuts at: 9514 9514
Size: 12178
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2326 3495 4549 6721 2326
Size: 1169 1054 2172 7783
Fragments arranged by size:
7783 2172 1169 1054
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 12178
XmaI C'CCGG_G
Cuts at: 1870 2107 2748 8367 8870 9503 1870
Size: 237 641 5619 503 633 4545
Fragments arranged by size:
5619 4545 641 633 503 237
XmnI GAAnn'nnTTC
Cuts at: 10307 10307
Size: 12178
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5809 8164 9986 276
Size: 53 83 186 936 4275 2355 1822 2468
Fragments arranged by size:
4275 2468 2355 1822 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BsiWI BstZ17I EcoNI
FseI FspAI KasI NarI PacI PspXI RsrII SfiI
SfoI SrfI SwaI