Restriction map for
pFN21AE3717
(Circular) (Six-base) MAPSORT of: pFN21AE3717.seq Check: 7452 from: 1 to: 11851
pFN21AE3717 11851 bp
With 182 enzymes: SgfI *
August 16, 2021 15:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2921 8723 9661 278
Size: 53 83 186 936 1385 5802 938 2468
Fragments arranged by size:
5802 2468 1385 938 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2709 4559 5208 5629 5911 8309 8766 9194
Size: 772 1850 649 421 282 2398 457 428
Cuts at: 9194 1937
Size: 4594
Fragments arranged by size:
4594 2398 1850 772 649 457 428 421
282
Acc65I G'GTAC_C
Cuts at: 3158 9172 3158
Size: 6014 5837
AclI AA'CG_TT
Cuts at: 1563 9978 10351 1563
Size: 8415 373 3063
Fragments arranged by size:
8415 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2813 2993 3146 3375 3846 3852 3912 5217
Size: 1181 180 153 229 471 6 60 1305
Cuts at: 5217 6162 6171 6336 6357 6933 7743 8463 9914
Size: 945 9 165 21 576 810 720 1451
Cuts at: 9914 11298 11566 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1451 1384 1305 1181 945 810 720
576 471 268 229 180 165 153 60
21 9 6
AflII C'TTAA_G
Cuts at: 820 1017 6022 8558 820
Size: 197 5005 2536 4113
Fragments arranged by size:
5005 4113 2536 197
AflIII A'CryG_T
Cuts at: 1203 10750 11727 1203
Size: 9547 977 1327
Fragments arranged by size:
9547 1327 977
AgeI A'CCGG_T
Cuts at: 10682 10682
Size: 11851
AhdI GACnn_n'nnGTC
Cuts at: 8194 10580 8194
Size: 2386 9465
AleI CACnn'nnGTG
Cuts at: 1166 4464 4985 6176 6455 1166
Size: 3298 521 1191 279 6562
Fragments arranged by size:
6562 3298 1191 521 279
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 11851
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4407 4439 5028 5060 5666 5698 7402 7434 4407
Size: 32 589 32 606 32 1704 32 8824
Fragments arranged by size:
8824 1704 606 589 32 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2423 4621 5852 6074 6380 7559 7814 8272 9594
Size: 2198 1231 222 306 1179 255 458 1322
Cuts at: 9594 11166 2423
Size: 1572 3108
Fragments arranged by size:
3108 2198 1572 1322 1231 1179 458 306
255 222
ApaI G_GGCC'C
Cuts at: 2456 8779 8840 2456
Size: 6323 61 5467
Fragments arranged by size:
6323 5467 61
ApaLI G'TGCA_C
Cuts at: 5214 9908 11064 5214
Size: 4694 1156 6001
Fragments arranged by size:
6001 4694 1156
ApoI r'AATT_y
Cuts at: 1493 5367 5447 9085 9160 9386 9538 11647 1493
Size: 3874 80 3638 75 226 152 2109 1697
Fragments arranged by size:
3874 3638 2109 1697 226 152 80 75
AseI AT'TA_AT
Cuts at: 160 1032 3695 10405 160
Size: 872 2663 6710 1606
Fragments arranged by size:
6710 2663 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11851
AvaI C'yCGr_G
Cuts at: 1870 1943 4005 4660 5471 8778 9176 1870
Size: 73 2062 655 811 3307 398 4545
Fragments arranged by size:
4545 3307 2062 811 655 398 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3911 3944 4847 4880 3911
Size: 33 903 33 10882
Fragments arranged by size:
10882 903 33 33
BamHI G'GATC_C
Cuts at: 3983 9181 9548 3983
Size: 5198 367 6286
Fragments arranged by size:
6286 5198 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2015 2169 2985 3158 7607
Size: 325 217 627 228 154 816 173 4449
Cuts at: 7607 8326 8609 9172 10627 618
Size: 719 283 563 1455 1842
Fragments arranged by size:
4449 1842 1455 816 719 627 563 325
283 228 217 173 154
BanII G_rGCy'C
Cuts at: 1391 2361 2456 5873 7018 8117 8779 8840 9170
Size: 970 95 3417 1145 1099 662 61 330
Cuts at: 9170 1391
Size: 4072
Fragments arranged by size:
4072 3417 1145 1099 970 662 330 95
61
BbeI G_GCGC'C
Cuts at: 2019 2173 2019
Size: 154 11697
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2819 2999 3985 5697 8028 8220 8380
Size: 971 920 180 986 1712 2331 192 160
Cuts at: 8380 928
Size: 4399
Fragments arranged by size:
4399 2331 1712 986 971 920 192 180
160
BbvCI CC'TCA_GC
Cuts at: 4221 4800 4221
Size: 579 11272
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 10042 10076 1903
Size: 34 8105 34 3678
Fragments arranged by size:
8105 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6357 6812 9743 10953 6357
Size: 455 2931 1210 7255
Fragments arranged by size:
7255 2931 1210 455
BclI T'GATC_A
Cuts at: 1548 3652 5611 7428 1548
Size: 2104 1959 1817 5971
Fragments arranged by size:
5971 2104 1959 1817
BfrBI ATG'CAT
Cuts at: 3381 3381
Size: 11851
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3087 6700 7799
Size: 107 122 71 631 605 1415 3613 1099
Cuts at: 7799 10462 136
Size: 2663 1525
Fragments arranged by size:
3613 2663 1525 1415 1099 631 605 122
107 71
BglII A'GATC_T
Cuts at: 3294 11846 3294
Size: 8552 3299
BlpI GC'TnA_GC
Cuts at: 6530 7017 8111 8703 9268 6530
Size: 487 1094 592 565 9113
Fragments arranged by size:
9113 1094 592 565 487
Bme1580I G_kGCm'C
Cuts at: 1790 2456 5218 5944 6138 7612 7780 8331 8738
Size: 666 2762 726 194 1474 168 551 407
Cuts at: 8738 8779 8823 8840 8969 9912 11068 1790
Size: 41 44 17 129 943 1156 2573
Fragments arranged by size:
2762 2573 1474 1156 943 726 666 551
407 194 168 129 44 41 17
BmgBI CAC'GTC
Cuts at: 1318 5939 1318
Size: 4621 7230
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2129 2492 3933
Size: 416 53 83 399 24 712 363 1441
Cuts at: 3933 4421 5823 7208 7360 7824 8327 9582 10540
Size: 488 1402 1385 152 464 503 1255 958
Cuts at: 10540 442
Size: 1753
Fragments arranged by size:
1753 1441 1402 1385 1255 958 712 503
488 464 416 399 363 152 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3834 1056
Size: 2778 9073
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2055 4350 5349 2055
Size: 2295 999 8557
Fragments arranged by size:
8557 2295 999
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2074 2303 2400 2645 4282 4839 6057 6177
Size: 835 229 97 245 1637 557 1218 120
Cuts at: 6177 6402 6684 7032 7058 8557 8622 10511 1239
Size: 225 282 348 26 1499 65 1889 2579
Fragments arranged by size:
2579 1889 1637 1499 1218 835 557 348
282 245 229 225 120 97 65 26
Bpu10I CC'TnA_GC
Cuts at: 4221 4800 6411 4221
Size: 579 1611 9661
Fragments arranged by size:
9661 1611 579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2651 3263 4120 4159 4320 4341 5733 7503 9976
Size: 612 857 39 161 21 1392 1770 2473
Cuts at: 9976 10841 11139 2651
Size: 865 298 3363
Fragments arranged by size:
3363 2473 1770 1392 865 857 612 298
161 39 21
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2366 2621 3099 5385 6200 6990 7034
Size: 617 867 255 478 2286 815 790 44
Cuts at: 7034 10514 882
Size: 3480 2219
Fragments arranged by size:
3480 2286 2219 867 815 790 617 478
255 44
BsaAI yAC'GTr
Cuts at: 493 1204 3518 9642 493
Size: 711 2314 6124 2702
Fragments arranged by size:
6124 2702 2314 711
BsaBI GATnn'nnATC
Cuts at: 2963 3912 8250 11845 2963
Size: 949 4338 3595 2969
Fragments arranged by size:
4338 3595 2969 949
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2016 2170 2918 8720
Size: 53 83 186 936 483 154 748 5802
Cuts at: 8720 9658 10040 10675 275
Size: 938 382 635 1451
Fragments arranged by size:
5802 1451 938 936 748 635 483 382
186 154 83 53
BsaWI w'CCGG_w
Cuts at: 2353 7282 8084 10284 10682 10956 11103 11514 2353
Size: 4929 802 2200 398 274 147 411 2690
Fragments arranged by size:
4929 2690 2200 802 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3013 3043 3447 3477 4237
Size: 30 165 30 1610 30 404 30 760
Cuts at: 4237 4267 4479 4509 5028 5058 5382 5412 5956
Size: 30 212 30 519 30 324 30 544
Cuts at: 5956 5986 6532 6562 6657 6687 7264 7294 7548
Size: 30 546 30 95 30 577 30 254
Cuts at: 7548 7578 1178
Size: 30 5451
Fragments arranged by size:
5451 1610 760 577 546 544 519 404
324 254 212 165 95 30 30 30
30 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2174 2546 3206 3749 4245 4539 5489 7929
Size: 988 372 660 543 496 294 950 2440
Cuts at: 7929 8161 1186
Size: 232 4876
Fragments arranged by size:
4876 2440 988 950 660 543 496 372
294 232
BseYI C'CCAG_C
Cuts at: 2313 2318 2406 2474 2505 4389 5055 5097 5181
Size: 5 88 68 31 1884 666 42 84
Cuts at: 5181 7325 7371 8350 8442 8493 9031 11054 11550
Size: 2144 46 979 92 51 538 2023 496
Cuts at: 11550 2313
Size: 2614
Fragments arranged by size:
2614 2144 2023 1884 979 666 538 496
92 88 84 68 51 46 42 31
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2262 3273 4289 4299 5009 6624 8493
Size: 573 526 1011 1016 10 710 1615 1869
Cuts at: 8493 8847 1163
Size: 354 4167
Fragments arranged by size:
4167 1869 1615 1016 1011 710 573 526
354 10
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2133 2211 9301 10062 11090 1540
Size: 275 186 132 78 7090 761 1028 2301
Fragments arranged by size:
7090 2301 1028 761 275 186 132 78
BsiHKAI G_wGCw'C
Cuts at: 2235 2350 4471 5218 7018 7064 8117 9912 9997
Size: 115 2121 747 1800 46 1053 1795 85
Cuts at: 9997 11068 2235
Size: 1071 3018
Fragments arranged by size:
3018 2121 1800 1795 1071 1053 747 115
85 46
BsmI GAATG_Cn'
Cuts at: 5727 9362 9455 5727
Size: 3635 93 8123
Fragments arranged by size:
8123 3635 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2235 2350 2361 2456 4471 5218 5873
Size: 399 445 115 11 95 2015 747 655
Cuts at: 5873 5944 6138 7018 7064 7612 7780 8117 8331
Size: 71 194 880 46 548 168 337 214
Cuts at: 8331 8738 8779 8823 8840 8969 9170 9912 9997
Size: 407 41 44 17 129 201 742 85
Cuts at: 9997 11068 1391
Size: 1071 2174
Fragments arranged by size:
2174 2015 1071 880 747 742 655 548
445 407 399 337 214 201 194 168
129 115 95 85 71 46 44 41
17 11
BspEI T'CCGG_A
Cuts at: 11514 11514
Size: 11851
BspHI T'CATG_A
Cuts at: 9740 11470 9740
Size: 1730 10121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3769 6268 7304 8280 9147 9206 844
Size: 2925 2499 1036 976 867 59 3489
Fragments arranged by size:
3489 2925 2499 1036 976 867 59
BsrBI CCG'CTC
Cuts at: 1394 1823 4495 9738 1394
Size: 429 2672 5243 3507
Fragments arranged by size:
5243 3507 2672 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5257 6030 10346 10520 65
Size: 1183 4009 773 4316 174 1396
Fragments arranged by size:
4316 4009 1396 1183 773 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2065 2147 2542 3077 3836 10495 10682
Size: 110 255 82 395 535 759 6659 187
Cuts at: 10682 1700
Size: 2869
Fragments arranged by size:
6659 2869 759 535 395 255 187 110
82
BsrGI T'GTAC_A
Cuts at: 96 6841 96
Size: 6745 5106
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11851
BssSI C'ACGA_G
Cuts at: 9911 10923 9911
Size: 1012 10839
BstAPI GCAn_nnn'nTGC
Cuts at: 794 7607 794
Size: 6813 5038
BstBI TT'CG_AA
Cuts at: 10655 10655
Size: 11851
BstEII G'GTnAC_C
Cuts at: 1184 8453 1184
Size: 7269 4582
BstXI CCAn_nnnn'nTGG
Cuts at: 2281 2638 4597 8513 2281
Size: 357 1959 3916 5619
Fragments arranged by size:
5619 3916 1959 357
BstYI r'GATC_y
Cuts at: 1972 2994 3294 3983 4422 7387 9181 9548 9933
Size: 1022 300 689 439 2965 1794 367 385
Cuts at: 9933 9950 11402 11488 11500 11846 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2965 1977 1794 1452 1022 689 439 385
367 346 300 86 17 12
BstZ17I GTA'TAC
Cuts at: 8310 8310
Size: 11851
Bsu36I CC'TnA_GG
Cuts at: 3900 3900
Size: 11851
BtgI C'CryG_G
Cuts at: 513 1065 2106 2127 2200 2328 2637 2736 3663
Size: 552 1041 21 73 128 309 99 927
Cuts at: 3663 4117 4767 6567 7461 513
Size: 454 650 1800 894 4903
Fragments arranged by size:
4903 1800 1041 927 894 650 552 454
309 128 99 73 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 11112
Fragments arranged by size:
11112 683 56
BtsI GCAGTG_nn'
Cuts at: 2274 9367 10161 10181 2274
Size: 7093 794 20 3944
Fragments arranged by size:
7093 3944 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11851
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4332 4367 7848 7883 529
Size: 35 3768 35 3481 35 4497
Fragments arranged by size:
4497 3768 3481 35 35 35
DraI TTT'AAA
Cuts at: 2800 2953 9155 2800
Size: 153 6202 5496
Fragments arranged by size:
6202 5496 153
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 10858 809
Size: 507 9542 1802
Fragments arranged by size:
9542 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2130 2208 2656
Size: 54 1425 50 275 18 300 78 448
Cuts at: 2656 9049 9298 10187 11816 8
Size: 6393 249 889 1629 43
Fragments arranged by size:
6393 1629 1425 889 448 300 275 249
78 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2130 2208 9298 1537
Size: 275 318 78 7090 4090
Fragments arranged by size:
7090 4090 318 275 78
EarI CTCTTCn'nnn_
Cuts at: 1353 2364 2613 3753 4897 6138 6658 7762 9781
Size: 1011 249 1140 1144 1241 520 1104 2019
Cuts at: 9781 1353
Size: 3423
Fragments arranged by size:
3423 2019 1241 1144 1140 1104 1011 520
249
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2234 10438 10812 10958 387
Size: 1064 138 645 8204 374 146 1280
Fragments arranged by size:
8204 1280 1064 645 374 146 138
EcoICRI GAG'CTC
Cuts at: 7016 8115 7016
Size: 1099 10752
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2074 2303 2400 2645 2813 2993 3146
Size: 393 442 229 97 245 168 180 153
Cuts at: 3146 3375 3846 3852 3912 4282 4839 5217 6057
Size: 229 471 6 60 370 557 378 840
Cuts at: 6057 6162 6171 6177 6336 6357 6402 6684 6933
Size: 105 9 6 159 21 45 282 249
Cuts at: 6933 7032 7058 7743 8463 8557 8622 9914 10511
Size: 99 26 685 720 94 65 1292 597
Cuts at: 10511 11298 11566 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1292 840 787 720 685 597 557
471 442 393 378 370 282 268 249
245 229 229 180 168 159 153 105
99 97 94 65 60 45 26 21
9 6 6
EcoNI CCTnn'n_nnAGG
Cuts at: 6666 7347 6666
Size: 681 11170
EcoO109I rG'GnC_Cy
Cuts at: 2453 5957 6407 6764 7352 7532 8269 8836 8837
Size: 3504 450 357 588 180 737 567 1
Cuts at: 8837 2453
Size: 5467
Fragments arranged by size:
5467 3504 737 588 567 450 357 180
1
EcoRI G'AATT_C
Cuts at: 9160 9538 9160
Size: 378 11473
EcoRV GAT'ATC
Cuts at: 2614 8203 2614
Size: 5589 6262
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6525 7985 6525
Size: 1460 10391
FspI TGC'GCA
Cuts at: 10357 10357
Size: 11851
HaeII r_GCGC'y
Cuts at: 1678 2019 2173 2574 9009 10998 1678
Size: 341 154 401 6435 1989 2531
Fragments arranged by size:
6435 2531 1989 401 341 154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3286 3318 3396 3428 3674
Size: 32 135 32 1714 32 78 32 246
Cuts at: 3674 3706 3975 4007 4147 4179 4237 4269 4479
Size: 32 269 32 140 32 58 32 210
Cuts at: 4479 4511 5513 5545 6584 6616 7631 7663 7790
Size: 32 1002 32 1039 32 1015 32 127
Cuts at: 7790 7822 8180 8212 10492 10524 10566 10598 1373
Size: 32 358 32 2280 32 42 32 2626
Fragments arranged by size:
2626 2280 1714 1039 1015 1002 358 269
246 210 140 135 127 78 58 42
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3214 3970 9195 9441 669
Size: 988 281 1276 756 5225 246 3079
Fragments arranged by size:
5225 3079 1276 988 756 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 11851
HpaI GTT'AAC
Cuts at: 9441 9441
Size: 11851
KasI G'GCGC_C
Cuts at: 2015 2169 2015
Size: 154 11697
KpnI G_GTAC'C
Cuts at: 3162 9176 3162
Size: 6014 5837
MfeI C'AATT_G
Cuts at: 7400 8770 9450 7400
Size: 1370 680 9801
Fragments arranged by size:
9801 1370 680
MluI A'CGCG_T
Cuts at: 11727 11727
Size: 11851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4718 4920 7496 7813 9082 9159 10965 11149
Size: 3413 202 2576 317 1269 77 1806 184
Cuts at: 11149 1305
Size: 2007
Fragments arranged by size:
3413 2576 2007 1806 1269 317 202 184
77
MscI TGG'CCA
Cuts at: 10 64 1489 1832 9051 10
Size: 54 1425 343 7219 2810
Fragments arranged by size:
7219 2810 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4464 4595 4985 5223 6176 6455 8025
Size: 648 3298 131 390 238 953 279 1570
Cuts at: 8025 8885 9809 10168 10327 518
Size: 860 924 359 159 2042
Fragments arranged by size:
3298 2042 1570 953 924 860 648 390
359 279 238 159 131
MspA1I CmG'CkG
Cuts at: 1606 2129 2330 2423 2559 3718 4367 4604 4652
Size: 523 201 93 136 1159 649 237 48
Cuts at: 4652 4720 6422 6517 7336 8110 8354 8545 9267
Size: 68 1702 95 819 774 244 191 722
Cuts at: 9267 9944 11092 11337 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1702 1159 1148 819 774 722 677
649 523 245 244 237 201 191 136
95 93 68 48
NaeI GCC'GGC
Cuts at: 2067 2544 2067
Size: 477 11374
NarI GG'CG_CC
Cuts at: 2016 2170 2016
Size: 154 11697
NcoI C'CATG_G
Cuts at: 513 1065 3663 4117 4767 7461 513
Size: 552 2598 454 650 2694 4903
Fragments arranged by size:
4903 2694 2598 650 552 454
NdeI CA'TA_TG
Cuts at: 387 4033 387
Size: 3646 8205
NgoMIV G'CCGG_C
Cuts at: 2065 2542 2065
Size: 477 11374
NheI G'CTAG_C
Cuts at: 1052 3830 1052
Size: 2778 9073
NotI GC'GGCC_GC
Cuts at: 2130 2208 9298 2130
Size: 78 7090 4683
Fragments arranged by size:
7090 4683 78
NsiI A_TGCA'T
Cuts at: 3383 3383
Size: 11851
NspI r_CATG'y
Cuts at: 1132 1229 3241 7025 9209 10754 1132
Size: 97 2012 3784 2184 1545 2229
Fragments arranged by size:
3784 2229 2184 2012 1545 97
PacI TTA_AT'TAA
Cuts at: 3699 3699
Size: 11851
PasI CC'CwG_GG
Cuts at: 1348 2432 4076 1348
Size: 1084 1644 9123
Fragments arranged by size:
9123 1644 1084
PciI A'CATG_T
Cuts at: 10750 10750
Size: 11851
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2185 9064 1108
Size: 1077 6879 3895
Fragments arranged by size:
6879 3895 1077
PfoI T'CCnGG_A
Cuts at: 6661 6943 7348 6661
Size: 282 405 11164
Fragments arranged by size:
11164 405 282
PmeI GTTT'AAAC
Cuts at: 9155 9155
Size: 11851
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5028 5060 7486 7518 9920 9952 11433 11465 5028
Size: 32 2426 32 2402 32 1481 32 5414
Fragments arranged by size:
5414 2426 2402 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 5957 6407 6764 7352 8269 5957
Size: 450 357 588 917 9539
Fragments arranged by size:
9539 917 588 450 357
PshAI GACnn'nnGTC
Cuts at: 1613 3111 3975 1613
Size: 1498 864 9489
Fragments arranged by size:
9489 1498 864
PsiI TTA'TAA
Cuts at: 9421 9421
Size: 11851
PspOMI G'GGCC_C
Cuts at: 2452 8775 8836 2452
Size: 6323 61 5467
Fragments arranged by size:
6323 5467 61
PstI C_TGCA'G
Cuts at: 830 2763 4707 5149 6265 6385 8277 8926 9203
Size: 1933 1944 442 1116 120 1892 649 277
Cuts at: 9203 830
Size: 3478
Fragments arranged by size:
3478 1944 1933 1892 1116 649 442 277
120
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11851
PvuII CAG'CTG
Cuts at: 4367 4604 4652 4720 6422 6517 7336 8110 8354
Size: 237 48 68 1702 95 819 774 244
Cuts at: 8354 8545 4367
Size: 191 7673
Fragments arranged by size:
7673 1702 819 774 244 237 191 95
68 48
SacI G_AGCT'C
Cuts at: 7018 8117 7018
Size: 1099 10752
SacII CC_GC'GG
Cuts at: 2130 2331 2130
Size: 201 11650
SalI G'TCGA_C
Cuts at: 1936 9193 1936
Size: 7257 4594
SapI GCTCTTCn'nnn_
Cuts at: 2613 2613
Size: 11851
SbfI CC_TGCA'GG
Cuts at: 9203 9203
Size: 11851
ScaI AGT'ACT
Cuts at: 4956 5119 6228 6394 6541 7129 10099 11811 4956
Size: 163 1109 166 147 588 2970 1712 4996
Fragments arranged by size:
4996 2970 1712 1109 588 166 163 147
SfcI C'TryA_G
Cuts at: 826 1045 2759 3923 4062 4703 4923 5145 5205
Size: 219 1714 1164 139 641 220 222 60
Cuts at: 5205 5859 6261 6381 6423 7854 8273 8922 9199
Size: 654 402 120 42 1431 419 649 277
Cuts at: 9199 10334 11015 11206 826
Size: 1135 681 191 1471
Fragments arranged by size:
1714 1471 1431 1164 1135 681 654 649
641 419 402 277 222 220 219 191
139 120 60 42
SfoI GGC'GCC
Cuts at: 2017 2171 2017
Size: 154 11697
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11851
SgrAI Cr'CCGG_yG
Cuts at: 1700 2542 1700
Size: 842 11009
SmaI CCC'GGG
Cuts at: 1872 4662 9178 1872
Size: 2790 4516 4545
Fragments arranged by size:
4545 4516 2790
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2666 3242 4005 4135 4174 4335
Size: 197 926 723 576 763 130 39 161
Cuts at: 4335 4356 5748 6022 7518 8558 9955 10856 11118
Size: 21 1392 274 1496 1040 1397 901 262
Cuts at: 11118 820
Size: 1553
Fragments arranged by size:
1553 1496 1397 1392 1040 926 901 763
723 576 274 262 197 161 130 39
21
SnaBI TAC'GTA
Cuts at: 493 3518 9642 493
Size: 3025 6124 2702
Fragments arranged by size:
6124 3025 2702
SpeI A'CTAG_T
Cuts at: 152 7048 7547 152
Size: 6896 499 4456
Fragments arranged by size:
6896 4456 499
SphI G_CATG'C
Cuts at: 1132 3241 7025 9209 1132
Size: 2109 3784 2184 3774
Fragments arranged by size:
3784 3774 2184 2109
SspI AAT'ATT
Cuts at: 5 52 2842 4882 9775 5
Size: 47 2790 2040 4893 2081
Fragments arranged by size:
4893 2790 2081 2040 47
StuI AGG'CCT
Cuts at: 1517 5921 1517
Size: 4404 7447
StyI C'CwwG_G
Cuts at: 513 1065 2574 2631 3663 4117 4408 4767 4786
Size: 552 1509 57 1032 454 291 359 19
Cuts at: 4786 6810 7461 7713 7827 8424 9290 513
Size: 2024 651 252 114 597 866 3074
Fragments arranged by size:
3074 2024 1509 1032 866 651 597 552
454 359 291 252 114 57 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2171 3101 4752 5015 5409 8891 9878
Size: 1255 591 930 1651 263 394 3482 987
Cuts at: 9878 9895 10048 10233 10678 325
Size: 17 153 185 445 1498
Fragments arranged by size:
3482 1651 1498 1255 987 930 591 445
394 263 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2163 4954 5117
Size: 274 80 33 51 1443 186 2791 163
Cuts at: 5117 6226 6335 6392 6539 6611 6841 7127 10097
Size: 1109 109 57 147 72 230 286 2970
Cuts at: 10097 11809 96
Size: 1712 138
Fragments arranged by size:
2970 2791 1712 1443 1109 286 274 230
186 163 147 138 109 80 72 57
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5956 5988 6285 6317 6657 6689 7062 7094 11811
Size: 32 297 32 340 32 373 32 4717
Cuts at: 11811 11843 5956
Size: 32 5964
Fragments arranged by size:
5964 4717 373 340 297 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 11851
XbaI T'CTAG_A
Cuts at: 6967 7381 9187 6967
Size: 414 1806 9631
Fragments arranged by size:
9631 1806 414
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6175 6175
Size: 11851
XhoI C'TCGA_G
Cuts at: 1943 4005 1943
Size: 2062 9789
XmaI C'CCGG_G
Cuts at: 1870 4660 9176 1870
Size: 2790 4516 4545
Fragments arranged by size:
4545 4516 2790
XmnI GAAnn'nnTTC
Cuts at: 8419 9980 8419
Size: 1561 10290
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2919 8721 9659 276
Size: 53 83 186 936 1385 5802 938 2468
Fragments arranged by size:
5802 2468 1385 938 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BsiWI BsmBI DraIII FseI
FspAI NruI PmlI PspXI PsrI RsrII SanDI SexAI
SfiI SrfI SwaI