Restriction map for
pFN21AE3714
(Circular) (Six-base) MAPSORT of: pFN21AE3714.seq Check: 9095 from: 1 to: 11215
pFN21AE3714 11215 bp
With 182 enzymes: SgfI *
March 28, 2012 12:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5093 5093
Size: 11215
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6705 9025 278
Size: 53 83 186 936 5169 2320 2468
Fragments arranged by size:
5169 2468 2320 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4625 8558 1937
Size: 2688 3933 4594
Fragments arranged by size:
4594 3933 2688
Acc65I G'GTAC_C
Cuts at: 3683 8536 3683
Size: 4853 6362
AclI AA'CG_TT
Cuts at: 1563 5634 9342 9715 1563
Size: 4071 3708 373 3063
Fragments arranged by size:
4071 3708 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2803 3312 4291 4602 5291 5732 6867 7650
Size: 1171 509 979 311 689 441 1135 783
Cuts at: 7650 8016 9278 10662 10930 1632
Size: 366 1262 1384 268 1917
Fragments arranged by size:
1917 1384 1262 1171 1135 979 783 689
509 441 366 311 268
AfeI AGC'GCT
Cuts at: 5561 5561
Size: 11215
AflII C'TTAA_G
Cuts at: 820 1017 7865 820
Size: 197 6848 4170
Fragments arranged by size:
6848 4170 197
AflIII A'CryG_T
Cuts at: 1203 4445 10114 11091 1203
Size: 3242 5669 977 1327
Fragments arranged by size:
5669 3242 1327 977
AgeI A'CCGG_T
Cuts at: 10046 10046
Size: 11215
AhdI GACnn_n'nnGTC
Cuts at: 9944 9944
Size: 11215
AleI CACnn'nnGTG
Cuts at: 1166 7115 1166
Size: 5949 5266
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4028 7521 7835 1184
Size: 2844 3493 314 4564
Fragments arranged by size:
4564 3493 2844 314
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7773 7805 7773
Size: 32 11183
AlwNI CAG_nnn'CTG
Cuts at: 2090 3776 4031 4355 5428 5626 5852 8008 8174
Size: 1686 255 324 1073 198 226 2156 166
Cuts at: 8174 8489 8958 10530 2090
Size: 315 469 1572 2775
Fragments arranged by size:
2775 2156 1686 1572 1073 469 324 315
255 226 198 166
ApaI G_GGCC'C
Cuts at: 2025 2025
Size: 11215
ApaLI G'TGCA_C
Cuts at: 7871 9272 10428 7871
Size: 1401 1156 8658
Fragments arranged by size:
8658 1401 1156
ApoI r'AATT_y
Cuts at: 1493 2189 3098 3396 3437 4506 4750 5377 5770
Size: 696 909 298 41 1069 244 627 393
Cuts at: 5770 6447 6513 6693 7194 7440 7613 7685 8111
Size: 677 66 180 501 246 173 72 426
Cuts at: 8111 8524 8750 8902 11011 1493
Size: 413 226 152 2109 1697
Fragments arranged by size:
2109 1697 1069 909 696 677 627 501
426 413 393 298 246 244 226 180
173 152 72 66 41
AseI AT'TA_AT
Cuts at: 160 1032 9769 160
Size: 872 8737 1606
Fragments arranged by size:
8737 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11215
AvaI C'yCGr_G
Cuts at: 1870 1943 2467 3159 3368 5994 7083 8540 1870
Size: 73 524 692 209 2626 1089 1457 4545
Fragments arranged by size:
4545 2626 1457 1089 692 524 209 73
BamHI G'GATC_C
Cuts at: 7015 8545 8912 7015
Size: 1530 367 9318
Fragments arranged by size:
9318 1530 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3683 4718 5230 5512 6290
Size: 325 217 627 1896 1035 512 282 778
Cuts at: 6290 7031 8536 9991 618
Size: 741 1505 1455 1842
Fragments arranged by size:
1896 1842 1505 1455 1035 778 741 627
512 325 282 217
BanII G_rGCy'C
Cuts at: 1391 2025 3723 4895 5961 6270 6729 7360 7914
Size: 634 1698 1172 1066 309 459 631 554
Cuts at: 7914 8534 1391
Size: 620 4072
Fragments arranged by size:
4072 1698 1172 1066 634 631 620 554
459 309
BbeI G_GCGC'C
Cuts at: 6294 6294
Size: 11215
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2916 3318 4215 5619 6629 6962 7716
Size: 971 1017 402 897 1404 1010 333 754
Cuts at: 7716 7831 928
Size: 115 4312
Fragments arranged by size:
4312 1404 1017 1010 971 897 754 402
333 115
BbvCI CC'TCA_GC
Cuts at: 6949 7176 6949
Size: 227 10988
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7077 7111 9406 9440 1903
Size: 34 5140 34 2295 34 3678
Fragments arranged by size:
5140 3678 2295 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7654 9107 10317 7654
Size: 1453 1210 8552
Fragments arranged by size:
8552 1453 1210
BclI T'GATC_A
Cuts at: 1548 8341 1548
Size: 6793 4422
BfrBI ATG'CAT
Cuts at: 4109 4109
Size: 11215
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2029 5949 6420
Size: 107 122 71 631 605 357 3920 471
Cuts at: 6420 9826 136
Size: 3406 1525
Fragments arranged by size:
3920 3406 1525 631 605 471 357 122
107 71
BglII A'GATC_T
Cuts at: 7983 11210 7983
Size: 3227 7988
BlpI GC'TnA_GC
Cuts at: 3717 6576 8632 3717
Size: 2859 2056 6300
Fragments arranged by size:
6300 2859 2056
Bme1580I G_kGCm'C
Cuts at: 1790 2025 2474 5233 5517 7034 7875 9276 10432
Size: 235 449 2759 284 1517 841 1401 1156
Cuts at: 10432 1790
Size: 2573
Fragments arranged by size:
2759 2573 1517 1401 1156 841 449 284
235
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 11215
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4065 4890 5229
Size: 416 53 83 399 24 2648 825 339
Cuts at: 5229 8212 8946 9904 442
Size: 2983 734 958 1753
Fragments arranged by size:
2983 2648 1753 958 825 734 416 399
339 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11215
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3975 4015 5142 3975
Size: 40 1127 10048
Fragments arranged by size:
10048 1127 40
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2510 2622 3005 3970 5346 6041 6114 6359
Size: 1271 112 383 965 1376 695 73 245
Cuts at: 6359 7344 9875 1239
Size: 985 2531 2579
Fragments arranged by size:
2579 2531 1376 1271 985 965 695 383
245 112 73
Bpu10I CC'TnA_GC
Cuts at: 5563 6565 6949 7176 5563
Size: 1002 384 227 9602
Fragments arranged by size:
9602 1002 384 227
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4258 4485 6131 8176 9340 10205 10503 4258
Size: 227 1646 2045 1164 865 298 4970
Fragments arranged by size:
4970 2045 1646 1164 865 298 227
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3162 4471 5587 5804 8486 9878 882
Size: 617 1663 1309 1116 217 2682 1392 2219
Fragments arranged by size:
2682 2219 1663 1392 1309 1116 617 217
BsaAI yAC'GTr
Cuts at: 493 1204 4629 9006 493
Size: 711 3425 4377 2702
Fragments arranged by size:
4377 3425 2702 711
BsaBI GATnn'nnATC
Cuts at: 11209 11209
Size: 11215
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6291 6702 9022 9404
Size: 53 83 186 936 4758 411 2320 382
Cuts at: 9404 10039 275
Size: 635 1451
Fragments arranged by size:
4758 2320 1451 936 635 411 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 9648 10046 10320 10467 10878 9648
Size: 398 274 147 411 9985
Fragments arranged by size:
9985 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2508 2538 3854 3884 6000
Size: 30 165 30 1105 30 1316 30 2116
Cuts at: 6000 6030 6826 6856 7773 7803 8100 8130 1178
Size: 30 796 30 917 30 297 30 4263
Fragments arranged by size:
4263 2116 1316 1105 917 796 297 165
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2482 2978 3256 3951 4055 5129 6473 6937
Size: 1296 496 278 695 104 1074 1344 464
Cuts at: 6937 7145 7781 8108 8111 1186
Size: 208 636 327 3 4290
Fragments arranged by size:
4290 1344 1296 1074 695 636 496 464
327 278 208 104 3
BseYI C'CCAG_C
Cuts at: 2888 3049 3176 4034 4805 5059 5433 5603 6494
Size: 161 127 858 771 254 374 170 891
Cuts at: 6494 8386 10418 10914 2888
Size: 1892 2032 496 3189
Fragments arranged by size:
3189 2032 1892 891 858 771 496 374
254 170 161 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2829 5189 5436 5475 7517 7527 7757
Size: 573 1093 2360 247 39 2042 10 230
Cuts at: 7757 1163
Size: 4621
Fragments arranged by size:
4621 2360 2042 1093 573 247 230 39
10
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8665 9426 10454 1540
Size: 275 186 6664 761 1028 2301
Fragments arranged by size:
6664 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2324 7360 7875 7914 9276 9361 10432 2324
Size: 5036 515 39 1362 85 1071 3107
Fragments arranged by size:
5036 3107 1362 1071 515 85 39
BsmI GAATG_Cn'
Cuts at: 2710 3658 7952 8726 8819 2710
Size: 948 4294 774 93 5106
Fragments arranged by size:
5106 4294 948 774 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3536 3536
Size: 11215
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2324 2474 3723 4895 5233 5517
Size: 399 235 299 150 1249 1172 338 284
Cuts at: 5517 5961 6270 6729 7034 7360 7875 7914 8534
Size: 444 309 459 305 326 515 39 620
Cuts at: 8534 9276 9361 10432 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1249 1172 1071 742 620 515 459
444 399 338 326 309 305 299 284
235 150 85 39
BspEI T'CCGG_A
Cuts at: 10878 10878
Size: 11215
BspHI T'CATG_A
Cuts at: 2761 6697 7444 9104 10834 2761
Size: 3936 747 1660 1730 3142
Fragments arranged by size:
3936 3142 1730 1660 747
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3054 5093 6194 8570 844
Size: 2210 2039 1101 2376 3489
Fragments arranged by size:
3489 2376 2210 2039 1101
BsrBI CCG'CTC
Cuts at: 1394 1823 9102 1394
Size: 429 7279 3507
Fragments arranged by size:
7279 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2054 3109 4419 5040 7881 9710 9884
Size: 1183 806 1055 1310 621 2841 1829 174
Cuts at: 9884 65
Size: 1396
Fragments arranged by size:
2841 1829 1396 1310 1183 1055 806 621
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6349 9859 10046 1700
Size: 110 4539 3510 187 2869
Fragments arranged by size:
4539 3510 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 5971 96
Size: 5875 5340
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11215
BssSI C'ACGA_G
Cuts at: 4296 5127 9275 10287 4296
Size: 831 4148 1012 5224
Fragments arranged by size:
5224 4148 1012 831
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4409 6470 6487 794
Size: 3615 2061 17 5522
Fragments arranged by size:
5522 3615 2061 17
BstBI TT'CG_AA
Cuts at: 10019 10019
Size: 11215
BstEII G'GTnAC_C
Cuts at: 1184 2738 4433 1184
Size: 1554 1695 7966
Fragments arranged by size:
7966 1695 1554
BstXI CCAn_nnnn'nTGG
Cuts at: 3233 6019 3233
Size: 2786 8429
BstYI r'GATC_y
Cuts at: 1972 6214 7015 7377 7983 8545 8912 9297 9314
Size: 4242 801 362 606 562 367 385 17
Cuts at: 9314 10766 10852 10864 11210 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
4242 1977 1452 801 606 562 385 367
362 346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3963 5088 6456 3963
Size: 1125 1368 8722
Fragments arranged by size:
8722 1368 1125
BtgI C'CryG_G
Cuts at: 513 1065 2715 2871 5517 5586 6030 7761 513
Size: 552 1650 156 2646 69 444 1731 3967
Fragments arranged by size:
3967 2646 1731 1650 552 444 156 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3096 487
Size: 683 56 1870 8606
Fragments arranged by size:
8606 1870 683 56
BtsI GCAGTG_nn'
Cuts at: 3192 3360 4410 6159 6559 7122 7168 8731 9525
Size: 168 1050 1749 400 563 46 1563 794
Cuts at: 9525 9545 3192
Size: 20 4862
Fragments arranged by size:
4862 1749 1563 1050 794 563 400 168
46 20
ClaI AT'CG_AT
Cuts at: 1554 2160 1554
Size: 606 10609
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 11180
DraI TTT'AAA
Cuts at: 4873 6172 8314 8350 8519 4873
Size: 1299 2142 36 169 7569
Fragments arranged by size:
7569 2142 1299 169 36
DraIII CAC_nnn'GTG
Cuts at: 5237 8206 5237
Size: 2969 8246
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4606 4621 10222 809
Size: 507 3290 15 5601 1802
Fragments arranged by size:
5601 3290 1802 507 15
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2874 4786 5750
Size: 54 1425 50 275 18 1044 1912 964
Cuts at: 5750 6351 7849 8662 9551 11180 8
Size: 601 1498 813 889 1629 43
Fragments arranged by size:
1912 1629 1498 1425 1044 964 889 813
601 275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8662 1537
Size: 275 6850 4090
Fragments arranged by size:
6850 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3084 5310 5367 5658 5949 6811 7305 7348
Size: 1731 2226 57 291 291 862 494 43
Cuts at: 7348 7671 8155 9145 1353
Size: 323 484 990 3423
Fragments arranged by size:
3423 2226 1731 990 862 494 484 323
291 291 57 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 9802 10176 10322 387
Size: 1064 138 8213 374 146 1280
Fragments arranged by size:
8213 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 7358 7912 7358
Size: 554 10661
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2510 2622 2803 3005 3312 3970 4291
Size: 393 878 112 181 202 307 658 321
Cuts at: 4291 4602 5291 5346 5732 6041 6114 6359 6867
Size: 311 689 55 386 309 73 245 508
Cuts at: 6867 7344 7650 8016 9278 9875 10662 10930 1239
Size: 477 306 366 1262 597 787 268 1524
Fragments arranged by size:
1524 1262 878 787 689 658 597 508
477 393 386 366 321 311 309 307
306 268 245 202 181 112 73 55
EcoNI CCTnn'n_nnAGG
Cuts at: 8010 8010
Size: 11215
EcoO109I rG'GnC_Cy
Cuts at: 2021 2022 2031 3305 7104 8486 2021
Size: 1 9 1274 3799 1382 4750
Fragments arranged by size:
4750 3799 1382 1274 9 1
EcoRI G'AATT_C
Cuts at: 2189 3098 3396 4750 6513 7685 8524 8902 2189
Size: 909 298 1354 1763 1172 839 378 4502
Fragments arranged by size:
4502 1763 1354 1172 909 839 378 298
EcoRV GAT'ATC
Cuts at: 5653 5653
Size: 11215
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3704 3768 5237 6006 3704
Size: 64 1469 769 8913
Fragments arranged by size:
8913 1469 769 64
FspI TGC'GCA
Cuts at: 9721 9721
Size: 11215
HaeII r_GCGC'y
Cuts at: 1678 5563 6294 10362 1678
Size: 3885 731 4068 2531
Fragments arranged by size:
4068 3885 2531 731
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2309 2341 3308 3339 3340
Size: 32 135 32 737 32 967 31 1
Cuts at: 3340 3371 3854 3886 3981 4013 4241 4273 4637
Size: 31 483 32 95 32 228 32 364
Cuts at: 4637 4669 4882 4914 5012 5044 5306 5338 5707
Size: 32 213 32 98 32 262 32 369
Cuts at: 5707 5739 5900 5932 6059 6091 7466 7498 9856
Size: 32 161 32 127 32 1375 32 2358
Cuts at: 9856 9888 9930 9962 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2358 1375 967 737 483 369 364
262 228 213 161 135 127 98 95
42 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 31 31 1
HincII GTy'rAC
Cuts at: 669 1657 1938 6112 6670 8559 8805 669
Size: 988 281 4174 558 1889 246 3079
Fragments arranged by size:
4174 3079 1889 988 558 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 11215
HpaI GTT'AAC
Cuts at: 6112 8805 6112
Size: 2693 8522
KasI G'GCGC_C
Cuts at: 6290 6290
Size: 11215
KpnI G_GTAC'C
Cuts at: 3687 8540 3687
Size: 4853 6362
MfeI C'AATT_G
Cuts at: 2339 4944 8814 2339
Size: 2605 3870 4740
Fragments arranged by size:
4740 3870 2605
MluI A'CGCG_T
Cuts at: 11091 11091
Size: 11215
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2205 2357 2532 3761 5154 5488 10329 10513
Size: 900 152 175 1229 1393 334 4841 184
Cuts at: 10513 1305
Size: 2007
Fragments arranged by size:
4841 2007 1393 1229 900 334 184 175
152
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2876 4788 5752 7851 10
Size: 54 1425 343 1044 1912 964 2099 3374
Fragments arranged by size:
3374 2099 1912 1425 1044 964 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2552 2673 3315 3795 5103 7115 7817
Size: 648 1386 121 642 480 1308 2012 702
Cuts at: 7817 7857 9173 9532 9691 518
Size: 40 1316 359 159 2042
Fragments arranged by size:
2042 2012 1386 1316 1308 702 648 642
480 359 159 121 40
MspA1I CmG'CkG
Cuts at: 1606 2038 2486 3551 6080 7628 7645 8631 9308
Size: 432 448 1065 2529 1548 17 986 677
Cuts at: 9308 10456 10701 1606
Size: 1148 245 2120
Fragments arranged by size:
2529 2120 1548 1148 1065 986 677 448
432 245 17
NaeI GCC'GGC
Cuts at: 6351 6351
Size: 11215
NarI GG'CG_CC
Cuts at: 6291 6291
Size: 11215
NcoI C'CATG_G
Cuts at: 513 1065 2715 513
Size: 552 1650 9013
Fragments arranged by size:
9013 1650 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 11215
NgoMIV G'CCGG_C
Cuts at: 6349 6349
Size: 11215
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11215
NotI GC'GGCC_GC
Cuts at: 8662 8662
Size: 11215
NsiI A_TGCA'T
Cuts at: 4111 4111
Size: 11215
NspI r_CATG'y
Cuts at: 1132 1229 3065 3910 4784 5102 8248 8573 10118
Size: 97 1836 845 874 318 3146 325 1545
Cuts at: 10118 1132
Size: 2229
Fragments arranged by size:
3146 2229 1836 1545 874 845 325 318
97
PasI CC'CwG_GG
Cuts at: 1348 7036 1348
Size: 5688 5527
PciI A'CATG_T
Cuts at: 10114 10114
Size: 11215
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2533 4055 5344 6883 7163 1108
Size: 1425 1522 1289 1539 280 5160
Fragments arranged by size:
5160 1539 1522 1425 1289 280
PfoI T'CCnGG_A
Cuts at: 3391 6018 3391
Size: 2627 8588
PmeI GTTT'AAAC
Cuts at: 8519 8519
Size: 11215
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5105 5137 7773 7805 9284 9316 10797 10829 5105
Size: 32 2636 32 1479 32 1481 32 5491
Fragments arranged by size:
5491 2636 1481 1479 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3305 7104 3305
Size: 3799 7416
PshAI GACnn'nnGTC
Cuts at: 1613 5579 5796 1613
Size: 3966 217 7032
Fragments arranged by size:
7032 3966 217
PsiI TTA'TAA
Cuts at: 2445 5720 8785 2445
Size: 3275 3065 4875
Fragments arranged by size:
4875 3275 3065
PspOMI G'GGCC_C
Cuts at: 2021 2021
Size: 11215
PstI C_TGCA'G
Cuts at: 830 5268 5294 7014 8567 830
Size: 4438 26 1720 1553 3478
Fragments arranged by size:
4438 3478 1720 1553 26
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11215
PvuII CAG'CTG
Cuts at: 2486 3551 7628 7645 2486
Size: 1065 4077 17 6056
Fragments arranged by size:
6056 4077 1065 17
SacI G_AGCT'C
Cuts at: 7360 7914 7360
Size: 554 10661
SalI G'TCGA_C
Cuts at: 1936 8557 1936
Size: 6621 4594
SanDI GG'GwC_CC
Cuts at: 7104 7104
Size: 11215
SapI GCTCTTCn'nnn_
Cuts at: 5949 7348 5949
Size: 1399 9816
SbfI CC_TGCA'GG
Cuts at: 8567 8567
Size: 11215
ScaI AGT'ACT
Cuts at: 4681 9463 11175 4681
Size: 4782 1712 4721
Fragments arranged by size:
4782 4721 1712
SexAI A'CCwGG_T
Cuts at: 6821 6821
Size: 11215
SfcI C'TryA_G
Cuts at: 826 1045 4515 5264 5290 6039 6210 7010 8085
Size: 219 3470 749 26 749 171 800 1075
Cuts at: 8085 8563 9698 10379 10570 826
Size: 478 1135 681 191 1471
Fragments arranged by size:
3470 1471 1135 1075 800 749 749 681
478 219 191 171 26
SfiI GGCCn_nnn'nGGCC
Cuts at: 2029 2029
Size: 11215
SfoI GGC'GCC
Cuts at: 6292 6292
Size: 11215
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11215
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11215
SmaI CCC'GGG
Cuts at: 1872 2469 5996 8542 1872
Size: 597 3527 2546 4545
Fragments arranged by size:
4545 3527 2546 597
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4273 4500 6146 7865 8155 9319
Size: 197 926 2330 227 1646 1719 290 1164
Cuts at: 9319 10220 10482 820
Size: 901 262 1553
Fragments arranged by size:
2330 1719 1646 1553 1164 926 901 290
262 227 197
SnaBI TAC'GTA
Cuts at: 493 9006 493
Size: 8513 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11215
SphI G_CATG'C
Cuts at: 1132 3910 5102 8573 1132
Size: 2778 1192 3471 3774
Fragments arranged by size:
3774 3471 2778 1192
SspI AAT'ATT
Cuts at: 5 52 4493 9139 5
Size: 47 4441 4646 2081
Fragments arranged by size:
4646 4441 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 11215
StyI C'CwwG_G
Cuts at: 513 1065 2715 4287 4388 4438 4740 4958 6960
Size: 552 1650 1572 101 50 302 218 2002
Cuts at: 6960 8654 513
Size: 1694 3074
Fragments arranged by size:
3074 2002 1694 1650 1572 552 302 218
101 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2567 3107 3111 5073 5248 8194 9242
Size: 1255 987 540 4 1962 175 2946 1048
Cuts at: 9242 9259 9412 9597 10042 325
Size: 17 153 185 445 1498
Fragments arranged by size:
2946 1962 1498 1255 1048 987 540 445
185 175 153 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2800 3671 4679
Size: 274 80 33 51 1443 823 871 1008
Cuts at: 4679 4777 5971 6243 6833 9461 11173 96
Size: 98 1194 272 590 2628 1712 138
Fragments arranged by size:
2628 1712 1443 1194 1008 871 823 590
274 272 138 98 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2508 2540 4361 4393 5244 5276 5445 5477 8100
Size: 32 1821 32 851 32 169 32 2623
Cuts at: 8100 8132 11175 11207 2508
Size: 32 3043 32 2516
Fragments arranged by size:
3043 2623 2516 1821 851 169 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3866 4255 5816 7123 1316
Size: 2550 389 1561 1307 5408
Fragments arranged by size:
5408 2550 1561 1307 389
XbaI T'CTAG_A
Cuts at: 5725 8551 5725
Size: 2826 8389
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2133 4762 8219 2133
Size: 2629 3457 5129
Fragments arranged by size:
5129 3457 2629
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 11215
XmaI C'CCGG_G
Cuts at: 1870 2467 5994 8540 1870
Size: 597 3527 2546 4545
Fragments arranged by size:
4545 3527 2546 597
XmnI GAAnn'nnTTC
Cuts at: 3325 5986 6447 6513 7788 9344 3325
Size: 2661 461 66 1275 1556 5196
Fragments arranged by size:
5196 2661 1556 1275 461 66
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6703 9023 276
Size: 53 83 186 936 5169 2320 2468
Fragments arranged by size:
5169 2468 2320 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BsiWI BstZ17I FseI FspAI NruI
PacI PmlI PspXI PsrI RsrII SacII SrfI SwaI