Restriction map for
pFN21AE3707
(Circular) (Six-base) MAPSORT of: pFN21AE3707.seq Check: 7167 from: 1 to: 5608
pFN21AE3707 5608 bp
With 182 enzymes: SgfI *
March 28, 2012 12:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3418 278
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2951 1937
Size: 1014 4594
Acc65I G'GTAC_C
Cuts at: 2929 2929
Size: 5608
AclI AA'CG_TT
Cuts at: 1563 3735 4108 1563
Size: 2172 373 3063
Fragments arranged by size:
3063 2172 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3671 5055 5323 1632
Size: 2039 1384 268 1917
Fragments arranged by size:
2039 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5411
AflIII A'CryG_T
Cuts at: 1203 4507 5484 1203
Size: 3304 977 1327
Fragments arranged by size:
3304 1327 977
AgeI A'CCGG_T
Cuts at: 4439 4439
Size: 5608
AhdI GACnn_n'nnGTC
Cuts at: 4337 4337
Size: 5608
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5608
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2210 1184
Size: 1026 4582
AlwNI CAG_nnn'CTG
Cuts at: 2518 2671 3351 4923 2518
Size: 153 680 1572 3203
Fragments arranged by size:
3203 1572 680 153
ApaLI G'TGCA_C
Cuts at: 3665 4821 3665
Size: 1156 4452
ApoI r'AATT_y
Cuts at: 1493 2917 3143 3295 5404 1493
Size: 1424 226 152 2109 1697
Fragments arranged by size:
2109 1697 1424 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4162 160
Size: 872 3130 1606
Fragments arranged by size:
3130 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
AvaI C'yCGr_G
Cuts at: 1870 1943 2933 1870
Size: 73 990 4545
Fragments arranged by size:
4545 990 73
BamHI G'GATC_C
Cuts at: 2938 3305 2938
Size: 367 5241
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2859 2929 4384 618
Size: 325 217 627 1072 70 1455 1842
Fragments arranged by size:
1842 1455 1072 627 325 217 70
BanII G_rGCy'C
Cuts at: 1391 2697 2817 2927 1391
Size: 1306 120 110 4072
Fragments arranged by size:
4072 1306 120 110
BbeI G_GCGC'C
Cuts at: 2863 2863
Size: 5608
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4637
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3799 3833 1903
Size: 34 50 34 1778 34 3678
Fragments arranged by size:
3678 1778 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3500 4710 3500
Size: 1210 4398
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5608
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2744 4219 136
Size: 107 122 71 631 605 1072 1475 1525
Fragments arranged by size:
1525 1475 1072 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5603 5603
Size: 5608
BlpI GC'TnA_GC
Cuts at: 3025 3025
Size: 5608
Bme1580I G_kGCm'C
Cuts at: 1790 2286 2599 3669 4825 1790
Size: 496 313 1070 1156 2573
Fragments arranged by size:
2573 1156 1070 496 313
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5608
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3339 4297 442
Size: 416 53 83 399 24 1922 958 1753
Fragments arranged by size:
1922 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2187 1056
Size: 1131 4477
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2301 4268 1239
Size: 1062 1967 2579
Fragments arranged by size:
2579 1967 1062
Bpu10I CC'TnA_GC
Cuts at: 2040 2808 2040
Size: 768 4840
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2855 3733 4598 4896 2855
Size: 878 865 298 3567
Fragments arranged by size:
3567 878 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2360 4271 882
Size: 617 861 1911 2219
Fragments arranged by size:
2219 1911 861 617
BsaAI yAC'GTr
Cuts at: 493 1204 3399 493
Size: 711 2195 2702
Fragments arranged by size:
2702 2195 711
BsaBI GATnn'nnATC
Cuts at: 5602 5602
Size: 5608
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2860 3415 3797 4432
Size: 53 83 186 936 1327 555 382 635
Cuts at: 4432 275
Size: 1451
Fragments arranged by size:
1451 1327 936 635 555 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2214 4041 4439 4713 4860 5271 2214
Size: 1827 398 274 147 411 2551
Fragments arranged by size:
2551 1827 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5383
Fragments arranged by size:
5383 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5608
BseYI C'CCAG_C
Cuts at: 2098 2139 2286 2311 2485 4811 5307 2098
Size: 41 147 25 174 2326 496 2399
Fragments arranged by size:
2399 2326 496 174 147 41 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2276 1163
Size: 573 540 4495
Fragments arranged by size:
4495 573 540
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2827 3058 3819 4847 1540
Size: 275 186 826 231 761 1028 2301
Fragments arranged by size:
2301 1028 826 761 275 231 186
BsiHKAI G_wGCw'C
Cuts at: 2817 3669 3754 4825 2817
Size: 852 85 1071 3600
Fragments arranged by size:
3600 1071 852 85
BsmI GAATG_Cn'
Cuts at: 3119 3212 3119
Size: 93 5515
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2286 2599 2697 2817 2927 3669 3754
Size: 399 496 313 98 120 110 742 85
Cuts at: 3754 4825 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 496 399 313 120 110
98 85
BspEI T'CCGG_A
Cuts at: 2214 5271 2214
Size: 3057 2551
BspHI T'CATG_A
Cuts at: 3497 5227 3497
Size: 1730 3878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2110 2366 2963 844
Size: 1266 256 597 3489
Fragments arranged by size:
3489 1266 597 256
BsrBI CCG'CTC
Cuts at: 1394 1823 3495 1394
Size: 429 1672 3507
Fragments arranged by size:
3507 1672 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2301 4103 4277 65
Size: 1183 1053 1802 174 1396
Fragments arranged by size:
1802 1396 1183 1053 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2562 2611 4252 4439 1700
Size: 110 752 49 1641 187 2869
Fragments arranged by size:
2869 1641 752 187 110 49
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5608
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5608
BssSI C'ACGA_G
Cuts at: 3668 4680 3668
Size: 1012 4596
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5608
BstBI TT'CG_AA
Cuts at: 4412 4412
Size: 5608
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5608
BstYI r'GATC_y
Cuts at: 1972 2025 2442 2469 2901 2938 3305 3690 3707
Size: 53 417 27 432 37 367 385 17
Cuts at: 3707 5159 5245 5257 5603 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 432 417 385 367 346 86
53 37 27 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2066 513
Size: 552 1001 4055
Fragments arranged by size:
4055 1001 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4869
Fragments arranged by size:
4869 683 56
BtsI GCAGTG_nn'
Cuts at: 2774 3124 3918 3938 2774
Size: 350 794 20 4444
Fragments arranged by size:
4444 794 350 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5608
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5573
DraI TTT'AAA
Cuts at: 2912 2912
Size: 5608
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4615 809
Size: 507 3299 1802
Fragments arranged by size:
3299 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3055 3944 5573
Size: 54 1425 50 275 18 1225 889 1629
Cuts at: 5573 8
Size: 43
Fragments arranged by size:
1629 1425 1225 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3055 1537
Size: 275 1243 4090
Fragments arranged by size:
4090 1243 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2433 3538 1353
Size: 1080 1105 3423
Fragments arranged by size:
3423 1105 1080
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4195 4569 4715 387
Size: 1064 138 2606 374 146 1280
Fragments arranged by size:
2606 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2815 2815
Size: 5608
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2301 3671 4268 5055 5323 1239
Size: 393 669 1370 597 787 268 1524
Fragments arranged by size:
1524 1370 787 669 597 393 268
EcoRI G'AATT_C
Cuts at: 2917 3295 2917
Size: 378 5230
FspI TGC'GCA
Cuts at: 4114 4114
Size: 5608
HaeII r_GCGC'y
Cuts at: 1678 2863 4755 1678
Size: 1185 1892 2531
Fragments arranged by size:
2531 1892 1185
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2005 2037 4249 4281 4323
Size: 32 135 32 433 32 2212 32 42
Cuts at: 4323 4355 1373
Size: 32 2626
Fragments arranged by size:
2626 2212 433 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2952 3198 669
Size: 988 281 1014 246 3079
Fragments arranged by size:
3079 1014 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5608
HpaI GTT'AAC
Cuts at: 3198 3198
Size: 5608
KasI G'GCGC_C
Cuts at: 2859 2859
Size: 5608
KpnI G_GTAC'C
Cuts at: 2933 2933
Size: 5608
MfeI C'AATT_G
Cuts at: 3207 3207
Size: 5608
MluI A'CGCG_T
Cuts at: 5484 5484
Size: 5608
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4722 4906 1305
Size: 3417 184 2007
Fragments arranged by size:
3417 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3786
Fragments arranged by size:
3786 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3566 3925 4084 518
Size: 648 2400 359 159 2042
Fragments arranged by size:
2400 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2143 3024 3701 4849 5094 1606
Size: 537 881 677 1148 245 2120
Fragments arranged by size:
2120 1148 881 677 537 245
NarI GG'CG_CC
Cuts at: 2860 2860
Size: 5608
NcoI C'CATG_G
Cuts at: 513 1065 2066 513
Size: 552 1001 4055
Fragments arranged by size:
4055 1001 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5608
NheI G'CTAG_C
Cuts at: 1052 2183 1052
Size: 1131 4477
NotI GC'GGCC_GC
Cuts at: 3055 3055
Size: 5608
NspI r_CATG'y
Cuts at: 1132 1229 2966 4511 1132
Size: 97 1737 1545 2229
Fragments arranged by size:
2229 1737 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2158 1348
Size: 810 4798
PciI A'CATG_T
Cuts at: 4507 4507
Size: 5608
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5608
PfoI T'CCnGG_A
Cuts at: 2445 2445
Size: 5608
PmeI GTTT'AAAC
Cuts at: 2912 2912
Size: 5608
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3677 3709 5190 5222 3677
Size: 32 1481 32 4063
Fragments arranged by size:
4063 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5608
PsiI TTA'TAA
Cuts at: 3178 3178
Size: 5608
PstI C_TGCA'G
Cuts at: 830 2121 2960 830
Size: 1291 839 3478
Fragments arranged by size:
3478 1291 839
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5608
PvuII CAG'CTG
Cuts at: 2143 2143
Size: 5608
SacI G_AGCT'C
Cuts at: 2817 2817
Size: 5608
SalI G'TCGA_C
Cuts at: 1936 2950 1936
Size: 1014 4594
SbfI CC_TGCA'GG
Cuts at: 2121 2960 2121
Size: 839 4769
ScaI AGT'ACT
Cuts at: 3856 5568 3856
Size: 1712 3896
SfcI C'TryA_G
Cuts at: 826 1045 2117 2542 2956 4091 4772 4963 826
Size: 219 1072 425 414 1135 681 191 1471
Fragments arranged by size:
1471 1135 1072 681 425 414 219 191
SfoI GGC'GCC
Cuts at: 2861 2861
Size: 5608
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5608
SmaI CCC'GGG
Cuts at: 1872 2935 1872
Size: 1063 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2870 3712 4613 4875 820
Size: 197 926 927 842 901 262 1553
Fragments arranged by size:
1553 927 926 901 842 262 197
SnaBI TAC'GTA
Cuts at: 493 3399 493
Size: 2906 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5608
SphI G_CATG'C
Cuts at: 1132 2966 1132
Size: 1834 3774
SspI AAT'ATT
Cuts at: 5 52 3532 5
Size: 47 3480 2081
Fragments arranged by size:
3480 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5608
StyI C'CwwG_G
Cuts at: 513 1065 2066 3047 513
Size: 552 1001 981 3074
Fragments arranged by size:
3074 1001 981 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2602 3635 3652 3805 3990 4435 325
Size: 1255 1022 1033 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1033 1022 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3854 5566 96
Size: 274 80 33 51 1443 1877 1712 138
Fragments arranged by size:
1877 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5568 5600 5568
Size: 32 5576
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5608
XbaI T'CTAG_A
Cuts at: 2944 2944
Size: 5608
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5608
XmaI C'CCGG_G
Cuts at: 1870 2933 1870
Size: 1063 4545
XmnI GAAnn'nnTTC
Cuts at: 3737 3737
Size: 5608
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3416 276
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI
XcmI