Restriction map for
pFN21AE3705
(Circular) (Six-base) MAPSORT of: pFN21AE3705.seq Check: 5912 from: 1 to: 5119
pFN21AE3705 5119 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2929 278
Size: 53 83 186 936 1393 2468
Fragments arranged by size:
2468 1393 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2462 1937
Size: 525 4594
Acc65I G'GTAC_C
Cuts at: 2440 2440
Size: 5119
AclI AA'CG_TT
Cuts at: 1563 3246 3619 1563
Size: 1683 373 3063
Fragments arranged by size:
3063 1683 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2262 3182 4566 4834 1632
Size: 630 920 1384 268 1917
Fragments arranged by size:
1917 1384 920 630 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4922
AflIII A'CryG_T
Cuts at: 1203 4018 4995 1203
Size: 2815 977 1327
Fragments arranged by size:
2815 1327 977
AgeI A'CCGG_T
Cuts at: 3950 3950
Size: 5119
AhdI GACnn_n'nnGTC
Cuts at: 3848 3848
Size: 5119
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5119
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5119
AlwNI CAG_nnn'CTG
Cuts at: 2396 2862 4434 2396
Size: 466 1572 3081
Fragments arranged by size:
3081 1572 466
ApaLI G'TGCA_C
Cuts at: 3176 4332 3176
Size: 1156 3963
ApoI r'AATT_y
Cuts at: 1493 2182 2374 2428 2654 2806 4915 1493
Size: 689 192 54 226 152 2109 1697
Fragments arranged by size:
2109 1697 689 226 192 152 54
AseI AT'TA_AT
Cuts at: 160 1032 3673 160
Size: 872 2641 1606
Fragments arranged by size:
2641 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5119
AvaI C'yCGr_G
Cuts at: 1870 1943 2444 1870
Size: 73 501 4545
Fragments arranged by size:
4545 501 73
BamHI G'GATC_C
Cuts at: 2449 2816 2449
Size: 367 4752
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2440 3895 618
Size: 325 217 627 653 1455 1842
Fragments arranged by size:
1842 1455 653 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2438 1391
Size: 1047 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4148
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2087 2121 3310 3344 1903
Size: 34 150 34 1189 34 3678
Fragments arranged by size:
3678 1189 150 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3011 4221 3011
Size: 1210 3909
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5119
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3730 136
Size: 107 122 71 631 605 2058 1525
Fragments arranged by size:
2058 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5114 5114
Size: 5119
BlpI GC'TnA_GC
Cuts at: 2536 2536
Size: 5119
Bme1580I G_kGCm'C
Cuts at: 1790 3180 4336 1790
Size: 1390 1156 2573
Fragments arranged by size:
2573 1390 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5119
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2850 3808 442
Size: 416 53 83 399 24 1433 958 1753
Fragments arranged by size:
1753 1433 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5119
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2418 3779 1239
Size: 1179 1361 2579
Fragments arranged by size:
2579 1361 1179
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3244 4109 4407 3244
Size: 865 298 3956
Fragments arranged by size:
3956 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2015 3782 882
Size: 617 516 1767 2219
Fragments arranged by size:
2219 1767 617 516
BsaAI yAC'GTr
Cuts at: 493 1204 2910 493
Size: 711 1706 2702
Fragments arranged by size:
2702 1706 711
BsaBI GATnn'nnATC
Cuts at: 5113 5113
Size: 5119
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2926 3308 3943 275
Size: 53 83 186 936 1393 382 635 1451
Fragments arranged by size:
1451 1393 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3552 3950 4224 4371 4782 3552
Size: 398 274 147 411 3889
Fragments arranged by size:
3889 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4894
Fragments arranged by size:
4894 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2027 2042 2281 1186
Size: 838 3 15 239 4024
Fragments arranged by size:
4024 838 239 15 3
BseYI C'CCAG_C
Cuts at: 4322 4818 4322
Size: 496 4623
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4546
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2569 3330 4358 1540
Size: 275 186 568 761 1028 2301
Fragments arranged by size:
2301 1028 761 568 275 186
BsiHKAI G_wGCw'C
Cuts at: 3180 3265 4336 3180
Size: 85 1071 3963
Fragments arranged by size:
3963 1071 85
BsmI GAATG_Cn'
Cuts at: 2630 2723 2630
Size: 93 5026
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2438 3180 3265 4336 1391
Size: 399 648 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 648 399 85
BspEI T'CCGG_A
Cuts at: 4782 4782
Size: 5119
BspHI T'CATG_A
Cuts at: 3008 4738 3008
Size: 1730 3389
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2474 844
Size: 1630 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3006 1394
Size: 429 1183 3507
Fragments arranged by size:
3507 1183 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2142 3614 3788 65
Size: 1183 894 1472 174 1396
Fragments arranged by size:
1472 1396 1183 894 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3763 3950 1700
Size: 110 1953 187 2869
Fragments arranged by size:
2869 1953 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5119
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5119
BssSI C'ACGA_G
Cuts at: 3179 4191 3179
Size: 1012 4107
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2216 794
Size: 1422 3697
BstBI TT'CG_AA
Cuts at: 3923 3923
Size: 5119
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5119
BstYI r'GATC_y
Cuts at: 1972 2449 2816 3201 3218 4670 4756 4768 5114
Size: 477 367 385 17 1452 86 12 346
Cuts at: 5114 1972
Size: 1977
Fragments arranged by size:
1977 1452 477 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3628
Fragments arranged by size:
3628 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4380
Fragments arranged by size:
4380 683 56
BtsI GCAGTG_nn'
Cuts at: 2276 2635 3429 3449 2276
Size: 359 794 20 3946
Fragments arranged by size:
3946 794 359 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5119
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5084
DraI TTT'AAA
Cuts at: 2423 2423
Size: 5119
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4126 809
Size: 507 2810 1802
Fragments arranged by size:
2810 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2148 2566 3455
Size: 54 1425 50 275 18 318 418 889
Cuts at: 3455 5084 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 418 318 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2566 1537
Size: 275 754 4090
Fragments arranged by size:
4090 754 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2301 3049 1353
Size: 948 748 3423
Fragments arranged by size:
3423 948 748
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2033 3706 4080 4226 387
Size: 1064 138 444 1673 374 146 1280
Fragments arranged by size:
1673 1280 1064 444 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2262 2418 3182 3779 4566 4834 1239
Size: 393 630 156 764 597 787 268 1524
Fragments arranged by size:
1524 787 764 630 597 393 268 156
EcoRI G'AATT_C
Cuts at: 2428 2806 2428
Size: 378 4741
FspI TGC'GCA
Cuts at: 3625 3625
Size: 5119
HaeII r_GCGC'y
Cuts at: 1678 4266 1678
Size: 2588 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3760 3792 3834 3866 1373
Size: 32 135 32 2188 32 42 32 2626
Fragments arranged by size:
2626 2188 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2463 2709 669
Size: 988 281 525 246 3079
Fragments arranged by size:
3079 988 525 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5119
HpaI GTT'AAC
Cuts at: 2709 2709
Size: 5119
KpnI G_GTAC'C
Cuts at: 2444 2444
Size: 5119
MfeI C'AATT_G
Cuts at: 2718 2718
Size: 5119
MluI A'CGCG_T
Cuts at: 4995 4995
Size: 5119
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2194 4233 4417 1305
Size: 889 2039 184 2007
Fragments arranged by size:
2039 2007 889 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3297
Fragments arranged by size:
3297 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3077 3436 3595 518
Size: 648 1911 359 159 2042
Fragments arranged by size:
2042 1911 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2535 3212 4360 4605 1606
Size: 929 677 1148 245 2120
Fragments arranged by size:
2120 1148 929 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3628
Fragments arranged by size:
3628 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5119
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5119
NotI GC'GGCC_GC
Cuts at: 2566 2566
Size: 5119
NspI r_CATG'y
Cuts at: 1132 1229 2219 2477 4022 1132
Size: 97 990 258 1545 2229
Fragments arranged by size:
2229 1545 990 258 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5119
PciI A'CATG_T
Cuts at: 4018 4018
Size: 5119
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5119
PmeI GTTT'AAAC
Cuts at: 2423 2423
Size: 5119
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2111 2143 3188 3220 4701 4733 2111
Size: 32 1045 32 1481 32 2497
Fragments arranged by size:
2497 1481 1045 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5119
PsiI TTA'TAA
Cuts at: 2689 2689
Size: 5119
PstI C_TGCA'G
Cuts at: 830 2471 830
Size: 1641 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5119
SalI G'TCGA_C
Cuts at: 1936 2461 1936
Size: 525 4594
SbfI CC_TGCA'GG
Cuts at: 2471 2471
Size: 5119
ScaI AGT'ACT
Cuts at: 3367 5079 3367
Size: 1712 3407
SfcI C'TryA_G
Cuts at: 826 1045 2467 3602 4283 4474 826
Size: 219 1422 1135 681 191 1471
Fragments arranged by size:
1471 1422 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5119
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5119
SmaI CCC'GGG
Cuts at: 1872 2446 1872
Size: 574 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3223 4124 4386 820
Size: 197 926 1280 901 262 1553
Fragments arranged by size:
1553 1280 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2910 493
Size: 2417 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5119
SphI G_CATG'C
Cuts at: 1132 2477 1132
Size: 1345 3774
SspI AAT'ATT
Cuts at: 5 52 3043 5
Size: 47 2991 2081
Fragments arranged by size:
2991 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5119
StyI C'CwwG_G
Cuts at: 513 1065 2004 2558 513
Size: 552 939 554 3074
Fragments arranged by size:
3074 939 554 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2039 3146 3163 3316 3501 3946 325
Size: 1255 459 1107 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1107 459 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3365 5077 96
Size: 274 80 33 51 1443 1388 1712 138
Fragments arranged by size:
1712 1443 1388 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5079 5111 5079
Size: 32 5087
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5119
XbaI T'CTAG_A
Cuts at: 2455 2455
Size: 5119
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5119
XmaI C'CCGG_G
Cuts at: 1870 2444 1870
Size: 574 4545
XmnI GAAnn'nnTTC
Cuts at: 3248 3248
Size: 5119
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2927 276
Size: 53 83 186 936 1393 2468
Fragments arranged by size:
2468 1393 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI