Restriction map for
pFN21AE3704
(Circular) (Six-base) MAPSORT of: pFN21AE3704.seq Check: 5430 from: 1 to: 8134
pFN21AE3704 8134 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4147 4147
Size: 8134
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5944 278
Size: 53 83 186 936 4408 2468
Fragments arranged by size:
4408 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2784 4290 5477 1937
Size: 847 1506 1187 4594
Fragments arranged by size:
4594 1506 1187 847
Acc65I G'GTAC_C
Cuts at: 5455 5455
Size: 8134
AclI AA'CG_TT
Cuts at: 1563 6261 6634 1563
Size: 4698 373 3063
Fragments arranged by size:
4698 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2186 2808 3057 3284 4426 4484 4634 4894
Size: 554 622 249 227 1142 58 150 260
Cuts at: 4894 6197 7581 7849 1632
Size: 1303 1384 268 1917
Fragments arranged by size:
1917 1384 1303 1142 622 554 268 260
249 227 150 58
AfeI AGC'GCT
Cuts at: 3620 3620
Size: 8134
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7937
AflIII A'CryG_T
Cuts at: 1203 7033 8010 1203
Size: 5830 977 1327
Fragments arranged by size:
5830 1327 977
AgeI A'CCGG_T
Cuts at: 6965 6965
Size: 8134
AhdI GACnn_n'nnGTC
Cuts at: 6863 6863
Size: 8134
AleI CACnn'nnGTG
Cuts at: 1166 3632 5009 5019 1166
Size: 2466 1377 10 4281
Fragments arranged by size:
4281 2466 1377 10
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8134
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2858 2890 2858
Size: 32 8102
AlwNI CAG_nnn'CTG
Cuts at: 5324 5877 7449 5324
Size: 553 1572 6009
Fragments arranged by size:
6009 1572 553
ApaLI G'TGCA_C
Cuts at: 5012 6191 7347 5012
Size: 1179 1156 5799
Fragments arranged by size:
5799 1179 1156
ApoI r'AATT_y
Cuts at: 1493 2170 2796 4575 5428 5443 5669 5821 7930
Size: 677 626 1779 853 15 226 152 2109
Cuts at: 7930 1493
Size: 1697
Fragments arranged by size:
2109 1779 1697 853 677 626 226 152
15
AseI AT'TA_AT
Cuts at: 160 1032 2655 5277 6688 160
Size: 872 1623 2622 1411 1606
Fragments arranged by size:
2622 1623 1606 1411 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8134
AvaI C'yCGr_G
Cuts at: 1870 1943 5459 1870
Size: 73 3516 4545
Fragments arranged by size:
4545 3516 73
BamHI G'GATC_C
Cuts at: 3088 3192 5464 5831 3088
Size: 104 2272 367 5391
Fragments arranged by size:
5391 2272 367 104
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3552 5455 6910 618
Size: 325 217 627 1765 1903 1455 1842
Fragments arranged by size:
1903 1842 1765 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3147 3302 5453 1391
Size: 1756 155 2151 4072
Fragments arranged by size:
4072 2151 1756 155
BbeI G_GCGC'C
Cuts at: 3556 3556
Size: 8134
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2244 2490 4554 4611 5160 928
Size: 971 345 246 2064 57 549 3902
Fragments arranged by size:
3902 2064 971 549 345 246 57
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6325 6359 1903
Size: 34 4388 34 3678
Fragments arranged by size:
4388 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2579 2877 3758 6026 7236 2579
Size: 298 881 2268 1210 3477
Fragments arranged by size:
3477 2268 1210 881 298
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8134
BfrBI ATG'CAT
Cuts at: 2399 3858 2399
Size: 1459 6675
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6745 136
Size: 107 122 71 631 605 5073 1525
Fragments arranged by size:
5073 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4396 8129 4396
Size: 3733 4401
BlpI GC'TnA_GC
Cuts at: 3301 5551 3301
Size: 2250 5884
Bme1580I G_kGCm'C
Cuts at: 1790 4167 5016 6195 7351 1790
Size: 2377 849 1179 1156 2573
Fragments arranged by size:
2573 2377 1179 1156 849
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8134
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2195 3235 3285
Size: 416 53 83 399 24 778 1040 50
Cuts at: 3285 4259 5865 6823 442
Size: 974 1606 958 1753
Fragments arranged by size:
1753 1606 1040 974 958 778 416 399
83 53 50 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8134
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2919 3786 2919
Size: 867 7267
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2549 2858 2889 3756 4193 4421 5091 6794
Size: 1310 309 31 867 437 228 670 1703
Cuts at: 6794 1239
Size: 2579
Fragments arranged by size:
2579 1703 1310 867 670 437 309 228
31
Bpu10I CC'TnA_GC
Cuts at: 4487 4487
Size: 8134
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2657 6259 7124 7422 2657
Size: 3602 865 298 3369
Fragments arranged by size:
3602 3369 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2907 4478 6797 882
Size: 617 1408 1571 2319 2219
Fragments arranged by size:
2319 2219 1571 1408 617
BsaAI yAC'GTr
Cuts at: 493 1204 5925 493
Size: 711 4721 2702
Fragments arranged by size:
4721 2702 711
BsaBI GATnn'nnATC
Cuts at: 8128 8128
Size: 8134
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3553 5941 6323 6958
Size: 53 83 186 936 2020 2388 382 635
Cuts at: 6958 275
Size: 1451
Fragments arranged by size:
2388 2020 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6567 6965 7239 7386 7797 6567
Size: 398 274 147 411 6904
Fragments arranged by size:
6904 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3596 3626 1178
Size: 30 165 30 2193 30 5686
Fragments arranged by size:
5686 2193 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2536 2911 3617 3797 4112 4335 1186
Size: 1350 375 706 180 315 223 4985
Fragments arranged by size:
4985 1350 706 375 315 223 180
BseYI C'CCAG_C
Cuts at: 5042 7337 7833 5042
Size: 2295 496 5343
Fragments arranged by size:
5343 2295 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2565 3656 3679 4108 4265 1163
Size: 573 829 1091 23 429 157 5032
Fragments arranged by size:
5032 1091 829 573 429 157 23
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5584 6345 7373 1540
Size: 275 186 3583 761 1028 2301
Fragments arranged by size:
3583 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3147 3302 3510 5016 6195 6280 7351 3147
Size: 155 208 1506 1179 85 1071 3930
Fragments arranged by size:
3930 1506 1179 1071 208 155 85
BsmI GAATG_Cn'
Cuts at: 2911 5645 5738 2911
Size: 2734 93 5307
Fragments arranged by size:
5307 2734 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2012 2012
Size: 8134
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3147 3302 3510 4167 5016 5453 6195
Size: 399 1357 155 208 657 849 437 742
Cuts at: 6195 6280 7351 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1357 1071 849 742 657 437 399
208 155 85
BspEI T'CCGG_A
Cuts at: 7797 7797
Size: 8134
BspHI T'CATG_A
Cuts at: 4966 6023 7753 4966
Size: 1057 1730 5347
Fragments arranged by size:
5347 1730 1057
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2206 3854 4147 5273 5489 844
Size: 1362 1648 293 1126 216 3489
Fragments arranged by size:
3489 1648 1362 1126 293 216
BsrBI CCG'CTC
Cuts at: 1394 1823 6021 1394
Size: 429 4198 3507
Fragments arranged by size:
4198 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2787 3730 4854 6629 6803 65
Size: 1183 1539 943 1124 1775 174 1396
Fragments arranged by size:
1775 1539 1396 1183 1124 943 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4039 6778 6965 1700
Size: 110 2229 2739 187 2869
Fragments arranged by size:
2869 2739 2229 187 110
BsrGI T'GTAC_A
Cuts at: 96 2026 96
Size: 1930 6204
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8134
BssSI C'ACGA_G
Cuts at: 3295 4434 6194 7206 3295
Size: 1139 1760 1012 4223
Fragments arranged by size:
4223 1760 1139 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5241 794
Size: 4447 3687
BstBI TT'CG_AA
Cuts at: 5171 6938 5171
Size: 1767 6367
BstEII G'GTnAC_C
Cuts at: 1184 4694 1184
Size: 3510 4624
BstYI r'GATC_y
Cuts at: 1972 3088 3163 3192 4008 4248 4396 5464 5831
Size: 1116 75 29 816 240 148 1068 367
Cuts at: 5831 6216 6233 7685 7771 7783 8129 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1116 1068 816 385 367 346
240 148 86 75 29 17 12
BstZ17I GTA'TAC
Cuts at: 2785 2785
Size: 8134
BtgI C'CryG_G
Cuts at: 513 1065 2004 2734 2878 3501 513
Size: 552 939 730 144 623 5146
Fragments arranged by size:
5146 939 730 623 552 144
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7395
Fragments arranged by size:
7395 683 56
BtsI GCAGTG_nn'
Cuts at: 3765 5650 6444 6464 3765
Size: 1885 794 20 5435
Fragments arranged by size:
5435 1885 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8134
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8099
DraI TTT'AAA
Cuts at: 5438 5438
Size: 8134
DraIII CAC_nnn'GTG
Cuts at: 4424 4424
Size: 8134
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7141 809
Size: 507 5825 1802
Fragments arranged by size:
5825 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5581 6470 8099
Size: 54 1425 50 275 18 3751 889 1629
Cuts at: 8099 8
Size: 43
Fragments arranged by size:
3751 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5581 1537
Size: 275 3769 4090
Fragments arranged by size:
4090 3769 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2667 2705 2970 3135 3783 4968 5088 5361
Size: 1314 38 265 165 648 1185 120 273
Cuts at: 5361 6064 1353
Size: 703 3423
Fragments arranged by size:
3423 1314 1185 703 648 273 265 165
120 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6721 7095 7241 387
Size: 1064 138 5132 374 146 1280
Fragments arranged by size:
5132 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3145 3300 3145
Size: 155 7979
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2186 2549 2808 2858 2889 3057 3284
Size: 393 554 363 259 50 31 168 227
Cuts at: 3284 3756 4193 4421 4426 4484 4634 4894 5091
Size: 472 437 228 5 58 150 260 197
Cuts at: 5091 6197 6794 7581 7849 1239
Size: 1106 597 787 268 1524
Fragments arranged by size:
1524 1106 787 597 554 472 437 393
363 268 260 259 228 227 197 168
150 58 50 31 5
EcoNI CCTnn'n_nnAGG
Cuts at: 3266 3266
Size: 8134
EcoRI G'AATT_C
Cuts at: 5428 5443 5821 5428
Size: 15 378 7741
Fragments arranged by size:
7741 378 15
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2518 2999 2518
Size: 481 7653
FspI TGC'GCA
Cuts at: 6640 6640
Size: 8134
HaeII r_GCGC'y
Cuts at: 1678 3556 3622 4107 7281 1678
Size: 1878 66 485 3174 2531
Fragments arranged by size:
3174 2531 1878 485 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2114 2146 3284 3316 3390
Size: 32 135 32 542 32 1138 32 74
Cuts at: 3390 3422 3652 3684 4388 4420 4480 4512 6775
Size: 32 230 32 704 32 60 32 2263
Cuts at: 6775 6807 6849 6881 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2263 1138 704 542 230 135 74
60 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4183 5478 5724 669
Size: 988 281 2245 1295 246 3079
Fragments arranged by size:
3079 2245 1295 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2577 4616 748
Size: 1829 2039 4266
Fragments arranged by size:
4266 2039 1829
HpaI GTT'AAC
Cuts at: 5724 5724
Size: 8134
KasI G'GCGC_C
Cuts at: 3552 3552
Size: 8134
KpnI G_GTAC'C
Cuts at: 5459 5459
Size: 8134
MfeI C'AATT_G
Cuts at: 5733 5733
Size: 8134
MluI A'CGCG_T
Cuts at: 8010 8010
Size: 8134
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2032 4113 4524 4836 4869 7248 7432 1305
Size: 727 2081 411 312 33 2379 184 2007
Fragments arranged by size:
2379 2081 2007 727 411 312 184 33
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6312
Fragments arranged by size:
6312 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2726 3632 4025 5009 5019 6092 6451
Size: 648 1560 906 393 984 10 1073 359
Cuts at: 6451 6610 518
Size: 159 2042
Fragments arranged by size:
2042 1560 1073 984 906 648 393 359
159 10
MspA1I CmG'CkG
Cuts at: 1606 3305 3653 4065 4454 4726 5550 6227 7375
Size: 1699 348 412 389 272 824 677 1148
Cuts at: 7375 7620 1606
Size: 245 2120
Fragments arranged by size:
2120 1699 1148 824 677 412 389 348
272 245
NaeI GCC'GGC
Cuts at: 4041 4041
Size: 8134
NarI GG'CG_CC
Cuts at: 3553 3553
Size: 8134
NcoI C'CATG_G
Cuts at: 513 1065 2004 3501 513
Size: 552 939 1497 5146
Fragments arranged by size:
5146 1497 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8134
NgoMIV G'CCGG_C
Cuts at: 4039 4039
Size: 8134
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8134
NotI GC'GGCC_GC
Cuts at: 5581 5581
Size: 8134
NsiI A_TGCA'T
Cuts at: 2401 3860 2401
Size: 1459 6675
NspI r_CATG'y
Cuts at: 1132 1229 3451 3858 4208 5211 5492 7037 1132
Size: 97 2222 407 350 1003 281 1545 2229
Fragments arranged by size:
2229 2222 1545 1003 407 350 281 97
PasI CC'CwG_GG
Cuts at: 1348 2230 1348
Size: 882 7252
PciI A'CATG_T
Cuts at: 7033 7033
Size: 8134
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2207 3009 1108
Size: 1099 802 6233
Fragments arranged by size:
6233 1099 802
PfoI T'CCnGG_A
Cuts at: 2862 2862
Size: 8134
PmeI GTTT'AAAC
Cuts at: 5438 5438
Size: 8134
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6203 6235 7716 7748 6203
Size: 32 1481 32 6589
Fragments arranged by size:
6589 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2553 3544 1613
Size: 940 991 6203
Fragments arranged by size:
6203 991 940
PsiI TTA'TAA
Cuts at: 5704 5704
Size: 8134
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2406 2438 2406
Size: 32 8102
PstI C_TGCA'G
Cuts at: 830 4416 5486 830
Size: 3586 1070 3478
Fragments arranged by size:
3586 3478 1070
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8134
PvuII CAG'CTG
Cuts at: 3305 4065 4454 3305
Size: 760 389 6985
Fragments arranged by size:
6985 760 389
SacI G_AGCT'C
Cuts at: 3147 3302 3147
Size: 155 7979
SalI G'TCGA_C
Cuts at: 1936 5476 1936
Size: 3540 4594
SapI GCTCTTCn'nnn_
Cuts at: 3135 5088 3135
Size: 1953 6181
SbfI CC_TGCA'GG
Cuts at: 5486 5486
Size: 8134
ScaI AGT'ACT
Cuts at: 3224 6382 8094 3224
Size: 3158 1712 3264
Fragments arranged by size:
3264 3158 1712
SexAI A'CCwGG_T
Cuts at: 2453 2453
Size: 8134
SfcI C'TryA_G
Cuts at: 826 1045 2423 3148 4412 5482 6617 7298 7489
Size: 219 1378 725 1264 1070 1135 681 191
Cuts at: 7489 826
Size: 1471
Fragments arranged by size:
1471 1378 1264 1135 1070 725 681 219
191
SfoI GGC'GCC
Cuts at: 3554 3554
Size: 8134
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8134
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8134
SmaI CCC'GGG
Cuts at: 1872 5461 1872
Size: 3589 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2636 6238 7139 7401 820
Size: 197 926 693 3602 901 262 1553
Fragments arranged by size:
3602 1553 926 901 693 262 197
SnaBI TAC'GTA
Cuts at: 493 5925 493
Size: 5432 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8134
SphI G_CATG'C
Cuts at: 1132 3858 4208 5492 1132
Size: 2726 350 1284 3774
Fragments arranged by size:
3774 2726 1284 350
SspI AAT'ATT
Cuts at: 5 52 2653 6058 5
Size: 47 2601 3405 2081
Fragments arranged by size:
3405 2601 2081 47
StuI AGG'CCT
Cuts at: 1517 2090 2469 4689 4932 4960 1517
Size: 573 379 2220 243 28 4691
Fragments arranged by size:
4691 2220 573 379 243 28
StyI C'CwwG_G
Cuts at: 513 1065 2004 2478 3196 3501 4690 5573 513
Size: 552 939 474 718 305 1189 883 3074
Fragments arranged by size:
3074 1189 939 883 718 552 474 305
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2199 3381 6161 6178 6331 6516 6961
Size: 1255 619 1182 2780 17 153 185 445
Cuts at: 6961 325
Size: 1498
Fragments arranged by size:
2780 1498 1255 1182 619 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2026 3222 5023
Size: 274 80 33 51 1443 49 1196 1801
Cuts at: 5023 6380 8092 96
Size: 1357 1712 138
Fragments arranged by size:
1801 1712 1443 1357 1196 274 138 80
51 49 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3184 3216 3596 3628 4420 4452 8094 8126 3184
Size: 32 380 32 792 32 3642 32 3192
Fragments arranged by size:
3642 3192 792 380 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4862 1316
Size: 3546 4588
XbaI T'CTAG_A
Cuts at: 5470 5470
Size: 8134
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2208 2208
Size: 8134
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8134
XmaI C'CCGG_G
Cuts at: 1870 5459 1870
Size: 3589 4545
XmnI GAAnn'nnTTC
Cuts at: 3075 5385 6263 3075
Size: 2310 878 4946
Fragments arranged by size:
4946 2310 878
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5942 276
Size: 53 83 186 936 4408 2468
Fragments arranged by size:
4408 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbvCI BsiWI BstXI Bsu36I
EcoO109I EcoRV FseI FspAI NruI PacI PmlI PpuMI
PspOMI PspXI RsrII SacII SanDI SfiI SrfI SwaI