Restriction map for
pFN21AE3703
(Circular) (Six-base) MAPSORT of: pFN21AE3703.seq Check: 225 from: 1 to: 9742
pFN21AE3703 9742 bp
With 182 enzymes: SgfI *
March 28, 2012 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5421 7552 278
Size: 53 83 186 936 3885 2131 2468
Fragments arranged by size:
3885 2468 2131 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7085 1937
Size: 5148 4594
Acc65I G'GTAC_C
Cuts at: 7063 7063
Size: 9742
AclI AA'CG_TT
Cuts at: 1563 7869 8242 1563
Size: 6306 373 3063
Fragments arranged by size:
6306 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3844 4190 5285 5324 7805 9189 9457 1632
Size: 2212 346 1095 39 2481 1384 268 1917
Fragments arranged by size:
2481 2212 1917 1384 1095 346 268 39
AfeI AGC'GCT
Cuts at: 6262 6262
Size: 9742
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9545
AflIII A'CryG_T
Cuts at: 1203 6543 6783 8641 9618 1203
Size: 5340 240 1858 977 1327
Fragments arranged by size:
5340 1858 1327 977 240
AgeI A'CCGG_T
Cuts at: 8573 8573
Size: 9742
AhdI GACnn_n'nnGTC
Cuts at: 8471 8471
Size: 9742
AleI CACnn'nnGTG
Cuts at: 1166 2370 5738 6902 1166
Size: 1204 3368 1164 4006
Fragments arranged by size:
4006 3368 1204 1164
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2874 5756 1184
Size: 1690 2882 5170
Fragments arranged by size:
5170 2882 1690
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6506 6538 6506
Size: 32 9710
AlwNI CAG_nnn'CTG
Cuts at: 5162 5198 5406 6034 7485 9057 5162
Size: 36 208 628 1451 1572 5847
Fragments arranged by size:
5847 1572 1451 628 208 36
ApaI G_GGCC'C
Cuts at: 2035 2439 2870 3315 4488 4626 4633 4670 5292
Size: 404 431 445 1173 138 7 37 622
Cuts at: 5292 6456 6913 2035
Size: 1164 457 4864
Fragments arranged by size:
4864 1173 1164 622 457 445 431 404
138 37 7
ApaLI G'TGCA_C
Cuts at: 6233 6401 7799 8955 6233
Size: 168 1398 1156 7020
Fragments arranged by size:
7020 1398 1156 168
ApoI r'AATT_y
Cuts at: 1493 4055 7051 7277 7429 9538 1493
Size: 2562 2996 226 152 2109 1697
Fragments arranged by size:
2996 2562 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6387 8296 160
Size: 872 5355 1909 1606
Fragments arranged by size:
5355 1909 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9742
AvaI C'yCGr_G
Cuts at: 1870 1943 3477 3908 3968 3990 4632 4663 5217
Size: 73 1534 431 60 22 642 31 554
Cuts at: 5217 5526 7067 1870
Size: 309 1541 4545
Fragments arranged by size:
4545 1541 1534 642 554 431 309 73
60 31 22
BamHI G'GATC_C
Cuts at: 7072 7439 7072
Size: 367 9375
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2211 2242 3179 3864 5981
Size: 325 217 627 424 31 937 685 2117
Cuts at: 5981 6139 6816 7063 8518 618
Size: 158 677 247 1455 1842
Fragments arranged by size:
2117 1842 1455 937 685 677 627 424
325 247 217 158 31
BanII G_rGCy'C
Cuts at: 1391 2035 2093 2439 2870 3315 3352 3507 3878
Size: 644 58 346 431 445 37 155 371
Cuts at: 3878 4179 4488 4574 4626 4633 4670 5292 5657
Size: 301 309 86 52 7 37 622 365
Cuts at: 5657 5807 6456 6913 6977 6999 7061 1391
Size: 150 649 457 64 22 62 4072
Fragments arranged by size:
4072 649 644 622 457 445 431 371
365 346 309 301 155 150 86 64
62 58 52 37 37 22 7
BbeI G_GCGC'C
Cuts at: 2246 3868 6820 2246
Size: 1622 2952 5168
Fragments arranged by size:
5168 2952 1622
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 8771
BbvCI CC'TCA_GC
Cuts at: 2672 4549 2672
Size: 1877 7865
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5971 6005 6791 6825 7933
Size: 34 50 34 3950 34 786 34 1108
Cuts at: 7933 7967 1903
Size: 34 3678
Fragments arranged by size:
3950 3678 1108 786 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7634 8844 7634
Size: 1210 8532
BclI T'GATC_A
Cuts at: 1548 6600 1548
Size: 5052 4690
BfrBI ATG'CAT
Cuts at: 2008 6332 2008
Size: 4324 5418
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2682 3177 3470
Size: 107 122 71 631 605 1010 495 293
Cuts at: 3470 5123 5652 6093 6837 6917 8353 136
Size: 1653 529 441 744 80 1436 1525
Fragments arranged by size:
1653 1525 1436 1010 744 631 605 529
495 441 293 122 107 80 71
BglII A'GATC_T
Cuts at: 3492 5787 9737 3492
Size: 2295 3950 3497
Fragments arranged by size:
3950 3497 2295
BlpI GC'TnA_GC
Cuts at: 2146 2427 6006 6083 7159 2146
Size: 281 3579 77 1076 4729
Fragments arranged by size:
4729 3579 1076 281 77
Bme1580I G_kGCm'C
Cuts at: 1790 2035 2214 2439 2870 3315 3605 4488 4626
Size: 245 179 225 431 445 290 883 138
Cuts at: 4626 4633 4670 5292 6144 6237 6405 6456 6534
Size: 7 37 622 852 93 168 51 78
Cuts at: 6534 6814 6913 6943 7803 8959 1790
Size: 280 99 30 860 1156 2573
Fragments arranged by size:
2573 1156 883 860 852 622 445 431
290 280 245 225 179 168 138 99
93 78 51 37 30 7
BmgBI CAC'GTC
Cuts at: 1318 7035 1318
Size: 5717 4025
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2609 4802 5479
Size: 416 53 83 399 24 1192 2193 677
Cuts at: 5479 5773 6256 6415 6953 7473 8431 442
Size: 294 483 159 538 520 958 1753
Fragments arranged by size:
2193 1753 1192 958 677 538 520 483
416 399 294 159 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9742
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2010 2107 2154 2220 2543 2700 2931 3094
Size: 771 97 47 66 323 157 231 163
Cuts at: 3094 3305 3801 4049 4448 4479 5127 5585 6872
Size: 211 496 248 399 31 648 458 1287
Cuts at: 6872 8402 1239
Size: 1530 2579
Fragments arranged by size:
2579 1530 1287 771 648 496 458 399
323 248 231 211 163 157 97 66
47 31
Bpu10I CC'TnA_GC
Cuts at: 2069 2672 4549 6879 6993 2069
Size: 603 1877 2330 114 4818
Fragments arranged by size:
4818 2330 1877 603 114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2570 7867 8732 9030 2570
Size: 5297 865 298 3282
Fragments arranged by size:
5297 3282 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3227 3295 3895 4754 5336 6245 6366
Size: 617 1728 68 600 859 582 909 121
Cuts at: 6366 8405 882
Size: 2039 2219
Fragments arranged by size:
2219 2039 1728 909 859 617 600 582
121 68
BsaAI yAC'GTr
Cuts at: 493 1204 6091 6544 6786 7533 493
Size: 711 4887 453 242 747 2702
Fragments arranged by size:
4887 2702 747 711 453 242
BsaBI GATnn'nnATC
Cuts at: 9736 9736
Size: 9742
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2243 3865 5418 6817
Size: 53 83 186 936 710 1622 1553 1399
Cuts at: 6817 6891 7549 7931 8566 275
Size: 74 658 382 635 1451
Fragments arranged by size:
1622 1553 1451 1399 936 710 658 635
382 186 83 74 53
BsaWI w'CCGG_w
Cuts at: 8175 8573 8847 8994 9405 8175
Size: 398 274 147 411 8512
Fragments arranged by size:
8512 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2516 2546 3534 3564 4022
Size: 30 165 30 1113 30 988 30 458
Cuts at: 4022 4052 4216 4246 4452 4482 6170 6200 6363
Size: 30 164 30 206 30 1688 30 163
Cuts at: 6363 6393 1178
Size: 30 4527
Fragments arranged by size:
4527 1688 1113 988 458 206 165 164
163 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2103 2390 2678 4058 4310 4889 5087 5528
Size: 917 287 288 1380 252 579 198 441
Cuts at: 5528 5891 5912 5921 6884 6949 1186
Size: 363 21 9 963 65 3979
Fragments arranged by size:
3979 1380 963 917 579 441 363 288
287 252 198 65 21 9
BseYI C'CCAG_C
Cuts at: 2078 2216 2319 2431 2463 2941 3692 3758 3763
Size: 138 103 112 32 478 751 66 5
Cuts at: 3763 3954 4388 4798 5047 5175 5552 6021 7024
Size: 191 434 410 249 128 377 469 1003
Cuts at: 7024 8945 9441 2078
Size: 1921 496 2379
Fragments arranged by size:
2379 1921 1003 751 496 478 469 434
410 377 249 191 138 128 112 103
66 32 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2025 2315 2642 5148 5762 6143 6380
Size: 573 289 290 327 2506 614 381 237
Cuts at: 6380 6431 1163
Size: 51 4474
Fragments arranged by size:
4474 2506 614 573 381 327 290 289
237 51
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6981 7192 7953 8981 1540
Size: 275 186 4980 211 761 1028 2301
Fragments arranged by size:
4980 2301 1028 761 275 211 186
BsiHKAI G_wGCw'C
Cuts at: 2093 2367 2556 3507 5807 6237 6243 6405 7803
Size: 274 189 951 2300 430 6 162 1398
Cuts at: 7803 7888 8959 2093
Size: 85 1071 2876
Fragments arranged by size:
2876 2300 1398 1071 951 430 274 189
162 85 6
BsmI GAATG_Cn'
Cuts at: 3249 5443 7253 7346 3249
Size: 2194 1810 93 5645
Fragments arranged by size:
5645 2194 1810 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2035 2093 2214 2367 2439 2556 2870
Size: 399 245 58 121 153 72 117 314
Cuts at: 2870 3315 3352 3507 3605 3878 4179 4488 4574
Size: 445 37 155 98 273 301 309 86
Cuts at: 4574 4626 4633 4670 5292 5657 5807 6144 6237
Size: 52 7 37 622 365 150 337 93
Cuts at: 6237 6243 6405 6456 6534 6814 6913 6943 6977
Size: 6 162 51 78 280 99 30 34
Cuts at: 6977 6999 7061 7803 7888 8959 1391
Size: 22 62 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 622 445 399 365 337
314 309 301 280 273 245 162 155
153 150 121 117 99 98 93 86
85 78 72 62 58 52 51 37
37 34 30 22 7 6
BspEI T'CCGG_A
Cuts at: 9405 9405
Size: 9742
BspHI T'CATG_A
Cuts at: 4089 7631 9361 4089
Size: 3542 1730 4470
Fragments arranged by size:
4470 3542 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5070 7097 844
Size: 4226 2027 3489
Fragments arranged by size:
4226 3489 2027
BsrBI CCG'CTC
Cuts at: 1394 1823 4774 5371 7629 1394
Size: 429 2951 597 2258 3507
Fragments arranged by size:
3507 2951 2258 597 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2300 5823 6435 8237 8411 65
Size: 1183 1052 3523 612 1802 174 1396
Fragments arranged by size:
3523 1802 1396 1183 1052 612 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2976 6419 7003 8386 8573 1700
Size: 110 1166 3443 584 1383 187 2869
Fragments arranged by size:
3443 2869 1383 1166 584 187 110
BsrGI T'GTAC_A
Cuts at: 96 5609 6129 96
Size: 5513 520 3709
Fragments arranged by size:
5513 3709 520
BssHII G'CGCG_C
Cuts at: 1925 5425 6920 1925
Size: 3500 1495 4747
Fragments arranged by size:
4747 3500 1495
BssSI C'ACGA_G
Cuts at: 6236 6377 7802 8814 6236
Size: 141 1425 1012 7164
Fragments arranged by size:
7164 1425 1012 141
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2302 2629 2920 5171 6034 6409 6726 794
Size: 1508 327 291 2251 863 375 317 3810
Fragments arranged by size:
3810 2251 1508 863 375 327 317 291
BstBI TT'CG_AA
Cuts at: 8546 8546
Size: 9742
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9742
BstXI CCAn_nnnn'nTGG
Cuts at: 2331 2838 4027 5695 2331
Size: 507 1189 1668 6378
Fragments arranged by size:
6378 1668 1189 507
BstYI r'GATC_y
Cuts at: 1972 3492 4062 5076 5307 5767 5787 7072 7439
Size: 1520 570 1014 231 460 20 1285 367
Cuts at: 7439 7824 7841 9293 9379 9391 9737 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1520 1452 1285 1014 570 460 385
367 346 231 86 20 17 12
Bsu36I CC'TnA_GG
Cuts at: 2656 3850 4615 2656
Size: 1194 765 7783
Fragments arranged by size:
7783 1194 765
BtgI C'CryG_G
Cuts at: 513 1065 2263 3034 3298 4278 5721 6069 513
Size: 552 1198 771 264 980 1443 348 4186
Fragments arranged by size:
4186 1443 1198 980 771 552 348 264
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3542 6744 487
Size: 683 56 2316 3202 3485
Fragments arranged by size:
3485 3202 2316 683 56
BtsI GCAGTG_nn'
Cuts at: 2022 2738 4902 6631 6796 7258 8052 8072 2022
Size: 716 2164 1729 165 462 794 20 3692
Fragments arranged by size:
3692 2164 1729 794 716 462 165 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9742
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9707
DraI TTT'AAA
Cuts at: 7046 7046
Size: 9742
DraIII CAC_nnn'GTG
Cuts at: 5243 5282 5722 6283 6409 5243
Size: 39 440 561 126 8576
Fragments arranged by size:
8576 561 440 126 39
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8749 809
Size: 507 7433 1802
Fragments arranged by size:
7433 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2446 2971 3301
Size: 54 1425 50 275 18 616 525 330
Cuts at: 3301 3726 4080 4281 4395 6308 6978 7189 8078
Size: 425 354 201 114 1913 670 211 889
Cuts at: 8078 9707 8
Size: 1629 43
Fragments arranged by size:
1913 1629 1425 889 670 616 525 425
354 330 275 211 201 114 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6978 7189 1537
Size: 275 5166 211 4090
Fragments arranged by size:
5166 4090 275 211
EarI CTCTTCn'nnn_
Cuts at: 1353 3495 3512 4116 4287 4647 4734 5361 5684
Size: 2142 17 604 171 360 87 627 323
Cuts at: 5684 7672 1353
Size: 1988 3423
Fragments arranged by size:
3423 2142 1988 627 604 360 323 171
87 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8329 8703 8849 387
Size: 1064 138 6740 374 146 1280
Fragments arranged by size:
6740 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2091 3505 5805 2091
Size: 1414 2300 6028
Fragments arranged by size:
6028 2300 1414
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2010 2107 2154 2220 2543 2700 2931
Size: 393 378 97 47 66 323 157 231
Cuts at: 2931 3094 3305 3801 3844 4049 4190 4448 4479
Size: 163 211 496 43 205 141 258 31
Cuts at: 4479 5127 5285 5324 5585 6872 7805 8402 9189
Size: 648 158 39 261 1287 933 597 787
Cuts at: 9189 9457 1239
Size: 268 1524
Fragments arranged by size:
1524 1287 933 787 648 597 496 393
378 323 268 261 258 231 211 205
163 158 157 141 97 66 47 43
39 31
EcoNI CCTnn'n_nnAGG
Cuts at: 3990 3990
Size: 9742
EcoO109I rG'GnC_Cy
Cuts at: 2031 2065 2082 2435 2436 2509 2585 2651 2668
Size: 34 17 353 1 73 76 66 17
Cuts at: 2668 2866 3008 3311 3312 3524 3575 3781 3935
Size: 198 142 303 1 212 51 206 154
Cuts at: 3935 3995 4485 4623 4667 5020 5115 5288 5289
Size: 60 490 138 44 353 95 173 1
Cuts at: 5289 5644 6452 6453 6909 7021 2031
Size: 355 808 1 456 112 4752
Fragments arranged by size:
4752 808 490 456 355 353 353 303
212 206 198 173 154 142 138 112
95 76 73 66 60 51 44 34
17 17 1 1 1 1
EcoRI G'AATT_C
Cuts at: 4055 7051 7429 4055
Size: 2996 378 6368
Fragments arranged by size:
6368 2996 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4195 5362 4195
Size: 1167 8575
FspI TGC'GCA
Cuts at: 5381 8248 5381
Size: 2867 6875
HaeII r_GCGC'y
Cuts at: 1678 2246 2403 2902 3261 3868 4566 6264 6820
Size: 568 157 499 359 607 698 1698 556
Cuts at: 6820 8889 1678
Size: 2069 2531
Fragments arranged by size:
2531 2069 1698 698 607 568 556 499
359 157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2493 2525 5068 5100 5570
Size: 32 135 32 921 32 2543 32 470
Cuts at: 5570 5602 5764 5796 5885 5917 8383 8415 8457
Size: 32 162 32 89 32 2466 32 42
Cuts at: 8457 8489 1373
Size: 32 2626
Fragments arranged by size:
2626 2543 2466 921 470 162 135 89
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 6193 7086 7332 669
Size: 988 281 4255 893 246 3079
Fragments arranged by size:
4255 3079 988 893 281 246
HindIII A'AGCT_T
Cuts at: 748 2419 3856 748
Size: 1671 1437 6634
Fragments arranged by size:
6634 1671 1437
HpaI GTT'AAC
Cuts at: 7332 7332
Size: 9742
KasI G'GCGC_C
Cuts at: 2242 3864 6816 2242
Size: 1622 2952 5168
Fragments arranged by size:
5168 2952 1622
KpnI G_GTAC'C
Cuts at: 7067 7067
Size: 9742
MfeI C'AATT_G
Cuts at: 7341 7341
Size: 9742
MluI A'CGCG_T
Cuts at: 9618 9618
Size: 9742
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3311 8856 9040 1305
Size: 2006 5545 184 2007
Fragments arranged by size:
5545 2007 2006 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2973 3728 4082 6310 10
Size: 54 1425 343 1141 755 354 2228 3442
Fragments arranged by size:
3442 2228 1425 1141 755 354 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2289 2370 2856 4094 5633 5738 6137
Size: 648 1123 81 486 1238 1539 105 399
Cuts at: 6137 6902 7700 8059 8218 518
Size: 765 798 359 159 2042
Fragments arranged by size:
2042 1539 1238 1123 798 765 648 486
399 359 159 105 81
MspA1I CmG'CkG
Cuts at: 1606 2911 3356 4027 4168 4466 4553 4829 4908
Size: 1305 445 671 141 298 87 276 79
Cuts at: 4908 6025 6127 6478 6931 7158 7835 8983 9228
Size: 1117 102 351 453 227 677 1148 245
Cuts at: 9228 1606
Size: 2120
Fragments arranged by size:
2120 1305 1148 1117 677 671 453 445
351 298 276 245 227 141 102 87
79
NaeI GCC'GGC
Cuts at: 7005 7005
Size: 9742
NarI GG'CG_CC
Cuts at: 2243 3865 6817 2243
Size: 1622 2952 5168
Fragments arranged by size:
5168 2952 1622
NcoI C'CATG_G
Cuts at: 513 1065 2263 3034 3298 513
Size: 552 1198 771 264 6957
Fragments arranged by size:
6957 1198 771 552 264
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9742
NgoMIV G'CCGG_C
Cuts at: 7003 7003
Size: 9742
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9742
NotI GC'GGCC_GC
Cuts at: 7189 7189
Size: 9742
NruI TCG'CGA
Cuts at: 3561 7039 3561
Size: 3478 6264
NsiI A_TGCA'T
Cuts at: 2010 6334 2010
Size: 4324 5418
NspI r_CATG'y
Cuts at: 1132 1229 5263 6082 7100 8645 1132
Size: 97 4034 819 1018 1545 2229
Fragments arranged by size:
4034 2229 1545 1018 819 97
PasI CC'CwG_GG
Cuts at: 1348 2729 3885 4567 4626 5231 1348
Size: 1381 1156 682 59 605 5859
Fragments arranged by size:
5859 1381 1156 682 605 59
PciI A'CATG_T
Cuts at: 8641 8641
Size: 9742
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2980 6423 1108
Size: 1872 3443 4427
Fragments arranged by size:
4427 3443 1872
PfoI T'CCnGG_A
Cuts at: 2307 5859 6315 2307
Size: 3552 456 5734
Fragments arranged by size:
5734 3552 456
PmeI GTTT'AAAC
Cuts at: 7046 7046
Size: 9742
PmlI CAC'GTG
Cuts at: 6091 6544 6091
Size: 453 9289
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5794 5826 7811 7843 9324 9356 5794
Size: 32 1985 32 1481 32 6180
Fragments arranged by size:
6180 1985 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2509 2585 3524 3781 5020 2509
Size: 76 939 257 1239 7231
Fragments arranged by size:
7231 1239 939 257 76
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9742
PsiI TTA'TAA
Cuts at: 7312 7312
Size: 9742
PspOMI G'GGCC_C
Cuts at: 2031 2435 2866 3311 4484 4622 4629 4666 5288
Size: 404 431 445 1173 138 7 37 622
Cuts at: 5288 6452 6909 2031
Size: 1164 457 4864
Fragments arranged by size:
4864 1173 1164 622 457 445 431 404
138 37 7
PstI C_TGCA'G
Cuts at: 830 2457 2641 4194 5067 5604 5874 6030 6621
Size: 1627 184 1553 873 537 270 156 591
Cuts at: 6621 6837 6958 7094 830
Size: 216 121 136 3478
Fragments arranged by size:
3478 1627 1553 873 591 537 270 216
184 156 136 121
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9742
PvuII CAG'CTG
Cuts at: 4466 4908 6025 6127 6478 4466
Size: 442 1117 102 351 7730
Fragments arranged by size:
7730 1117 442 351 102
SacI G_AGCT'C
Cuts at: 2093 3507 5807 2093
Size: 1414 2300 6028
Fragments arranged by size:
6028 2300 1414
SalI G'TCGA_C
Cuts at: 1936 7084 1936
Size: 5148 4594
SanDI GG'GwC_CC
Cuts at: 3524 3524
Size: 9742
SapI GCTCTTCn'nnn_
Cuts at: 3495 3512 4647 5361 3495
Size: 17 1135 714 7876
Fragments arranged by size:
7876 1135 714 17
SbfI CC_TGCA'GG
Cuts at: 6837 7094 6837
Size: 257 9485
ScaI AGT'ACT
Cuts at: 4442 7990 9702 4442
Size: 3548 1712 4482
Fragments arranged by size:
4482 3548 1712
SexAI A'CCwGG_T
Cuts at: 6789 6789
Size: 9742
SfcI C'TryA_G
Cuts at: 826 1045 2453 2637 4190 5063 5600 5870 6026
Size: 219 1408 184 1553 873 537 270 156
Cuts at: 6026 6617 6833 6954 7090 8225 8906 9097 826
Size: 591 216 121 136 1135 681 191 1471
Fragments arranged by size:
1553 1471 1408 1135 873 681 591 537
270 219 216 191 184 156 136 121
SfiI GGCCn_nnn'nGGCC
Cuts at: 5123 5123
Size: 9742
SfoI GGC'GCC
Cuts at: 2244 3866 6818 2244
Size: 1622 2952 5168
Fragments arranged by size:
5168 2952 1622
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9742
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9742
SmaI CCC'GGG
Cuts at: 1872 3970 4634 4665 5528 7069 1872
Size: 2098 664 31 863 1541 4545
Fragments arranged by size:
4545 2098 1541 863 664 31
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2549 7846 8747 9009 820
Size: 197 926 606 5297 901 262 1553
Fragments arranged by size:
5297 1553 926 901 606 262 197
SnaBI TAC'GTA
Cuts at: 493 7533 493
Size: 7040 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9742
SphI G_CATG'C
Cuts at: 1132 7100 1132
Size: 5968 3774
SspI AAT'ATT
Cuts at: 5 52 6340 7666 5
Size: 47 6288 1326 2081
Fragments arranged by size:
6288 2081 1326 47
StuI AGG'CCT
Cuts at: 1517 5849 6831 6884 1517
Size: 4332 982 53 4375
Fragments arranged by size:
4375 4332 982 53
StyI C'CwwG_G
Cuts at: 513 1065 2059 2263 2505 3034 3298 4413 4869
Size: 552 994 204 242 529 264 1115 456
Cuts at: 4869 5010 5016 5121 5295 5442 7181 513
Size: 141 6 105 174 147 1739 3074
Fragments arranged by size:
3074 1739 1115 994 552 529 456 264
242 204 174 147 141 105 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2943 4301 4697 5149 5566 5750 6397
Size: 1255 1363 1358 396 452 417 184 647
Cuts at: 6397 7769 7786 7939 8124 8569 325
Size: 1372 17 153 185 445 1498
Fragments arranged by size:
1498 1372 1363 1358 1255 647 452 445
417 396 185 184 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4440 5609 6129
Size: 274 80 33 51 1443 2463 1169 520
Cuts at: 6129 7988 9700 96
Size: 1859 1712 138
Fragments arranged by size:
2463 1859 1712 1443 1169 520 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2516 2548 3534 3566 4216 4248 6363 6395 9702
Size: 32 986 32 650 32 2115 32 3307
Cuts at: 9702 9734 2516
Size: 32 2524
Fragments arranged by size:
3307 2524 2115 986 650 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9742
XbaI T'CTAG_A
Cuts at: 7078 7078
Size: 9742
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3444 5560 5692 5695 3444
Size: 2116 132 3 7491
Fragments arranged by size:
7491 2116 132 3
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9742
XmaI C'CCGG_G
Cuts at: 1870 3968 4632 4663 5526 7067 1870
Size: 2098 664 31 863 1541 4545
Fragments arranged by size:
4545 2098 1541 863 664 31
XmnI GAAnn'nnTTC
Cuts at: 3338 4110 7871 3338
Size: 772 3761 5209
Fragments arranged by size:
5209 3761 772
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5419 7550 276
Size: 53 83 186 936 3885 2131 2468
Fragments arranged by size:
3885 2468 2131 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BplI BsiWI BsmBI BstZ17I
EcoRV FseI FspAI PacI PspXI PsrI RsrII SacII
SrfI SwaI