Restriction map for
pFN21AE3695
(Circular) (Six-base) MAPSORT of: pFN21AE3695.seq Check: 4583 from: 1 to: 6709
pFN21AE3695 6709 bp
With 182 enzymes: SgfI *
March 28, 2012 13:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2135 2135
Size: 6709
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4519 278
Size: 53 83 186 936 2983 2468
Fragments arranged by size:
2983 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4052 1937
Size: 2115 4594
Acc65I G'GTAC_C
Cuts at: 3033 4030 3033
Size: 997 5712
AclI AA'CG_TT
Cuts at: 1563 4836 5209 1563
Size: 3273 373 3063
Fragments arranged by size:
3273 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2234 4772 6156 6424 1632
Size: 602 2538 1384 268 1917
Fragments arranged by size:
2538 1917 1384 602 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6512
AflIII A'CryG_T
Cuts at: 1203 2293 2295 2598 5608 6585 1203
Size: 1090 2 303 3010 977 1327
Fragments arranged by size:
3010 1327 1090 977 303 2
AgeI A'CCGG_T
Cuts at: 5540 5540
Size: 6709
AhdI GACnn_n'nnGTC
Cuts at: 2971 5438 2971
Size: 2467 4242
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6709
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2972 1184
Size: 1788 4921
AlwNI CAG_nnn'CTG
Cuts at: 2677 2740 2968 3904 4452 6024 2677
Size: 63 228 936 548 1572 3362
Fragments arranged by size:
3362 1572 936 548 228 63
ApaI G_GGCC'C
Cuts at: 2058 2058
Size: 6709
ApaLI G'TGCA_C
Cuts at: 3656 4766 5922 3656
Size: 1110 1156 4443
Fragments arranged by size:
4443 1156 1110
ApoI r'AATT_y
Cuts at: 1493 4018 4244 4396 6505 1493
Size: 2525 226 152 2109 1697
Fragments arranged by size:
2525 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5263 160
Size: 872 4231 1606
Fragments arranged by size:
4231 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6709
AvaI C'yCGr_G
Cuts at: 1870 1943 2555 3037 3288 4034 1870
Size: 73 612 482 251 746 4545
Fragments arranged by size:
4545 746 612 482 251 73
BamHI G'GATC_C
Cuts at: 4039 4406 4039
Size: 367 6342
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3033 3187 3248 3421 4030
Size: 325 217 627 1246 154 61 173 609
Cuts at: 4030 5485 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1246 627 609 325 217 173
154 61
BanII G_rGCy'C
Cuts at: 1391 2058 2313 2644 2835 3371 3470 3638 4028
Size: 667 255 331 191 536 99 168 390
Cuts at: 4028 1391
Size: 4072
Fragments arranged by size:
4072 667 536 390 331 255 191 168
99
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2078 928
Size: 971 179 5559
Fragments arranged by size:
5559 971 179
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3905 3939 4900 4934 1903
Size: 34 1968 34 961 34 3678
Fragments arranged by size:
3678 1968 961 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4601 5811 4601
Size: 1210 5499
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6709
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2062 3196 3375
Size: 107 122 71 631 605 390 1134 179
Cuts at: 3375 5320 136
Size: 1945 1525
Fragments arranged by size:
1945 1525 1134 631 605 390 179 122
107 71
BglII A'GATC_T
Cuts at: 3603 6704 3603
Size: 3101 3608
BlpI GC'TnA_GC
Cuts at: 2168 3161 3905 4126 2168
Size: 993 744 221 4751
Fragments arranged by size:
4751 993 744 221
Bme1580I G_kGCm'C
Cuts at: 1790 2058 3426 3660 4770 5926 1790
Size: 268 1368 234 1110 1156 2573
Fragments arranged by size:
2573 1368 1156 1110 268 234
BmgBI CAC'GTC
Cuts at: 1318 2236 2599 2908 1318
Size: 918 363 309 5119
Fragments arranged by size:
5119 918 363 309
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2939 3672 4440
Size: 416 53 83 399 24 1522 733 768
Cuts at: 4440 5398 442
Size: 958 1753
Fragments arranged by size:
1753 1522 958 768 733 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6709
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2087 2699 2909 3448 3608 5369 1239
Size: 848 612 210 539 160 1761 2579
Fragments arranged by size:
2579 1761 848 612 539 210 160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4834 5699 5997 4834
Size: 865 298 5546
Fragments arranged by size:
5546 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2516 2937 3237 3878 5372 882
Size: 617 1017 421 300 641 1494 2219
Fragments arranged by size:
2219 1494 1017 641 617 421 300
BsaAI yAC'GTr
Cuts at: 493 1204 2296 4500 493
Size: 711 1092 2204 2702
Fragments arranged by size:
2702 2204 1092 711
BsaBI GATnn'nnATC
Cuts at: 2207 6703 2207
Size: 4496 2213
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2345 2492 2613 2766
Size: 53 83 186 936 812 147 121 153
Cuts at: 2766 3506 4516 4898 5533 275
Size: 740 1010 382 635 1451
Fragments arranged by size:
1451 1010 936 812 740 635 382 186
153 147 121 83 53
BsaWI w'CCGG_w
Cuts at: 3021 5142 5540 5814 5961 6372 3021
Size: 2121 398 274 147 411 3358
Fragments arranged by size:
3358 2121 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2393 2423 3164 3194 3503
Size: 30 165 30 990 30 741 30 309
Cuts at: 3503 3533 1178
Size: 30 4354
Fragments arranged by size:
4354 990 741 309 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2993 2996 3002 3244 3527 3611 1186
Size: 1807 3 6 242 283 84 4284
Fragments arranged by size:
4284 1807 283 242 84 6 3
BseYI C'CCAG_C
Cuts at: 2077 2107 2882 3216 3407 3857 3897 3930 5912
Size: 30 775 334 191 450 40 33 1982
Cuts at: 5912 6408 2077
Size: 496 2378
Fragments arranged by size:
2378 1982 775 496 450 334 191 40
33 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2231 2885 1163
Size: 573 495 654 4987
Fragments arranged by size:
4987 654 573 495
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2587 4159 4920 5948 1540
Size: 275 186 586 1572 761 1028 2301
Fragments arranged by size:
2301 1572 1028 761 586 275 186
BsiHKAI G_wGCw'C
Cuts at: 2229 2313 2793 3162 3638 3660 3911 4770 4855
Size: 84 480 369 476 22 251 859 85
Cuts at: 4855 5926 2229
Size: 1071 3012
Fragments arranged by size:
3012 1071 859 480 476 369 251 85
84 22
BsmI GAATG_Cn'
Cuts at: 4220 4313 4220
Size: 93 6616
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 2229 2313 2644 2793 2835 3162
Size: 399 268 171 84 331 149 42 327
Cuts at: 3162 3371 3426 3470 3638 3660 3911 4028 4770
Size: 209 55 44 168 22 251 117 742
Cuts at: 4770 4855 5926 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 331 327 268 251
209 171 168 149 117 85 84 55
44 42 22
BspEI T'CCGG_A
Cuts at: 6372 6372
Size: 6709
BspHI T'CATG_A
Cuts at: 4598 6328 4598
Size: 1730 4979
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2135 2491 2976 3066 4064 844
Size: 1291 356 485 90 998 3489
Fragments arranged by size:
3489 1291 998 485 356 90
BsrBI CCG'CTC
Cuts at: 1394 1823 2197 2874 4596 1394
Size: 429 374 677 1722 3507
Fragments arranged by size:
3507 1722 677 429 374
BsrDI GCAATG_nn'
Cuts at: 65 1248 5204 5378 65
Size: 1183 3956 174 1396
Fragments arranged by size:
3956 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2061 3117 3760 5353 5540 1700
Size: 110 251 1056 643 1593 187 2869
Fragments arranged by size:
2869 1593 1056 643 251 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6709
BssHII G'CGCG_C
Cuts at: 1925 2031 1925
Size: 106 6603
BssSI C'ACGA_G
Cuts at: 4769 5781 4769
Size: 1012 5697
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2968 794
Size: 2174 4535
BstBI TT'CG_AA
Cuts at: 5513 5513
Size: 6709
BstEII G'GTnAC_C
Cuts at: 1184 3292 1184
Size: 2108 4601
BstXI CCAn_nnnn'nTGG
Cuts at: 3211 3211
Size: 6709
BstYI r'GATC_y
Cuts at: 1972 2425 2868 3603 4039 4406 4791 4808 6260
Size: 453 443 735 436 367 385 17 1452
Cuts at: 6260 6346 6358 6704 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 735 453 443 436 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2349 3256 2349
Size: 907 5802
BtgI C'CryG_G
Cuts at: 513 1065 3150 3732 513
Size: 552 2085 582 3490
Fragments arranged by size:
3490 2085 582 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2458 487
Size: 683 56 1232 4738
Fragments arranged by size:
4738 1232 683 56
BtsI GCAGTG_nn'
Cuts at: 2116 4225 5019 5039 2116
Size: 2109 794 20 3786
Fragments arranged by size:
3786 2109 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6709
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6674
DraI TTT'AAA
Cuts at: 4013 4013
Size: 6709
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3535 5716 809
Size: 507 2219 2181 1802
Fragments arranged by size:
2219 2181 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2139 2584 3134
Size: 54 1425 50 275 18 309 445 550
Cuts at: 3134 3223 4156 5045 6674 8
Size: 89 933 889 1629 43
Fragments arranged by size:
1629 1425 933 889 550 445 309 275
89 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2584 4156 1537
Size: 275 772 1572 4090
Fragments arranged by size:
4090 1572 772 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2318 4639 1353
Size: 965 2321 3423
Fragments arranged by size:
3423 2321 965
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2609 5296 5670 5816 387
Size: 1064 138 1020 2687 374 146 1280
Fragments arranged by size:
2687 1280 1064 1020 374 146 138
EcoICRI GAG'CTC
Cuts at: 2311 3636 2311
Size: 1325 5384
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2234 2699 2909 3448 3608 4772
Size: 393 455 147 465 210 539 160 1164
Cuts at: 4772 5369 6156 6424 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1164 787 597 539 465 455 393
268 210 160 147
EcoNI CCTnn'n_nnAGG
Cuts at: 3310 3590 3310
Size: 280 6429
EcoO109I rG'GnC_Cy
Cuts at: 2055 2535 2965 3055 3854 2055
Size: 480 430 90 799 4910
Fragments arranged by size:
4910 799 480 430 90
EcoRI G'AATT_C
Cuts at: 4018 4396 4018
Size: 378 6331
EcoRV GAT'ATC
Cuts at: 3781 3781
Size: 6709
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3475 3475
Size: 6709
FspI TGC'GCA
Cuts at: 5215 5215
Size: 6709
HaeII r_GCGC'y
Cuts at: 1678 5856 1678
Size: 4178 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2957 2989 3067 3099 3164
Size: 32 135 32 1385 32 78 32 65
Cuts at: 3164 3196 3503 3535 3664 3696 5350 5382 5424
Size: 32 307 32 129 32 1654 32 42
Cuts at: 5424 5456 1373
Size: 32 2626
Fragments arranged by size:
2626 1654 1385 307 135 129 78 65
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2155 4053 4299 669
Size: 988 281 217 1898 246 3079
Fragments arranged by size:
3079 1898 988 281 246 217
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6709
HpaI GTT'AAC
Cuts at: 4299 4299
Size: 6709
KpnI G_GTAC'C
Cuts at: 3037 4034 3037
Size: 997 5712
MfeI C'AATT_G
Cuts at: 4308 4308
Size: 6709
MluI A'CGCG_T
Cuts at: 6585 6585
Size: 6709
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2499 3394 3527 3546 5823 6007 1305
Size: 1194 895 133 19 2277 184 2007
Fragments arranged by size:
2277 2007 1194 895 184 133 19
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2141 10
Size: 54 1425 343 309 4578
Fragments arranged by size:
4578 1425 343 309 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3155 3209 4667 5026 5185 518
Size: 648 1989 54 1458 359 159 2042
Fragments arranged by size:
2042 1989 1458 648 359 159 54
MspA1I CmG'CkG
Cuts at: 1606 2529 2674 3246 3901 4125 4802 5950 6195
Size: 923 145 572 655 224 677 1148 245
Cuts at: 6195 1606
Size: 2120
Fragments arranged by size:
2120 1148 923 677 655 572 245 224
145
NcoI C'CATG_G
Cuts at: 513 1065 3150 513
Size: 552 2085 4072
Fragments arranged by size:
4072 2085 552
NdeI CA'TA_TG
Cuts at: 387 2414 2807 387
Size: 2027 393 4289
Fragments arranged by size:
4289 2027 393
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6709
NotI GC'GGCC_GC
Cuts at: 4156 4156
Size: 6709
NruI TCG'CGA
Cuts at: 2207 2207
Size: 6709
NspI r_CATG'y
Cuts at: 1132 1229 2710 2923 4067 5612 1132
Size: 97 1481 213 1144 1545 2229
Fragments arranged by size:
2229 1545 1481 1144 213 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6709
PciI A'CATG_T
Cuts at: 5608 5608
Size: 6709
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2137 1108
Size: 1029 5680
PfoI T'CCnGG_A
Cuts at: 3015 3015
Size: 6709
PmeI GTTT'AAAC
Cuts at: 4013 4013
Size: 6709
PmlI CAC'GTG
Cuts at: 2296 2296
Size: 6709
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4778 4810 6291 6323 4778
Size: 32 1481 32 5164
Fragments arranged by size:
5164 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2535 2965 3055 2535
Size: 430 90 6189
Fragments arranged by size:
6189 430 90
PshAI GACnn'nnGTC
Cuts at: 1613 3347 1613
Size: 1734 4975
PsiI TTA'TAA
Cuts at: 4279 4279
Size: 6709
PspOMI G'GGCC_C
Cuts at: 2054 2054
Size: 6709
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3809 3841 3809
Size: 32 6677
PstI C_TGCA'G
Cuts at: 830 2502 2964 4061 830
Size: 1672 462 1097 3478
Fragments arranged by size:
3478 1672 1097 462
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6709
PvuII CAG'CTG
Cuts at: 2529 2674 3901 2529
Size: 145 1227 5337
Fragments arranged by size:
5337 1227 145
SacI G_AGCT'C
Cuts at: 2313 3638 2313
Size: 1325 5384
SalI G'TCGA_C
Cuts at: 1936 4051 1936
Size: 2115 4594
SanDI GG'GwC_CC
Cuts at: 2535 2535
Size: 6709
SapI GCTCTTCn'nnn_
Cuts at: 2318 2318
Size: 6709
SbfI CC_TGCA'GG
Cuts at: 2502 4061 2502
Size: 1559 5150
ScaI AGT'ACT
Cuts at: 4957 6669 4957
Size: 1712 4997
SexAI A'CCwGG_T
Cuts at: 2280 2322 2280
Size: 42 6667
SfcI C'TryA_G
Cuts at: 826 1045 2498 2960 4057 5192 5873 6064 826
Size: 219 1453 462 1097 1135 681 191 1471
Fragments arranged by size:
1471 1453 1135 1097 681 462 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6709
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6709
SmaI CCC'GGG
Cuts at: 1872 3290 4036 1872
Size: 1418 746 4545
Fragments arranged by size:
4545 1418 746
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4813 5714 5976 820
Size: 197 926 2870 901 262 1553
Fragments arranged by size:
2870 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4500 493
Size: 4007 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6709
SphI G_CATG'C
Cuts at: 1132 2923 4067 1132
Size: 1791 1144 3774
Fragments arranged by size:
3774 1791 1144
SspI AAT'ATT
Cuts at: 5 52 2273 4633 5
Size: 47 2221 2360 2081
Fragments arranged by size:
2360 2221 2081 47
StuI AGG'CCT
Cuts at: 1517 3261 1517
Size: 1744 4965
StyI C'CwwG_G
Cuts at: 513 1065 3150 3311 3336 4148 513
Size: 552 2085 161 25 812 3074
Fragments arranged by size:
3074 2085 812 552 161 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3225 4736 4753 4906 5091 5536 325
Size: 1255 1645 1511 17 153 185 445 1498
Fragments arranged by size:
1645 1511 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3723 4955 6667
Size: 274 80 33 51 1443 1746 1232 1712
Cuts at: 6667 96
Size: 138
Fragments arranged by size:
1746 1712 1443 1232 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2393 2425 3462 3494 6669 6701 2393
Size: 32 1037 32 3175 32 2401
Fragments arranged by size:
3175 2401 1037 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6709
XbaI T'CTAG_A
Cuts at: 4045 4045
Size: 6709
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3955 3955
Size: 6709
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6709
XmaI C'CCGG_G
Cuts at: 1870 3288 4034 1870
Size: 1418 746 4545
Fragments arranged by size:
4545 1418 746
XmnI GAAnn'nnTTC
Cuts at: 2366 4838 2366
Size: 2472 4237
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4517 276
Size: 53 83 186 936 2983 2468
Fragments arranged by size:
2983 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NruI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I DraIII FseI FspAI
KasI NaeI NarI NgoMIV NsiI PacI PspXI RsrII
SacII SfiI SfoI SrfI SwaI