Restriction map for
pFN21AE3691
(Circular) (Six-base) MAPSORT of: pFN21AE3691.seq Check: 9688 from: 1 to: 6670
pFN21AE3691 6670 bp
With 182 enzymes: SgfI *
March 28, 2012 12:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3350 3350
Size: 6670
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4480 278
Size: 53 83 186 936 2944 2468
Fragments arranged by size:
2944 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4013 1937
Size: 2076 4594
Acc65I G'GTAC_C
Cuts at: 3141 3991 3141
Size: 850 5820
AclI AA'CG_TT
Cuts at: 1563 4797 5170 1563
Size: 3234 373 3063
Fragments arranged by size:
3234 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3223 3622 4733 6117 6385 1632
Size: 1591 399 1111 1384 268 1917
Fragments arranged by size:
1917 1591 1384 1111 399 268
AflII C'TTAA_G
Cuts at: 820 1017 2750 820
Size: 197 1733 4740
Fragments arranged by size:
4740 1733 197
AflIII A'CryG_T
Cuts at: 1203 5569 6546 1203
Size: 4366 977 1327
Fragments arranged by size:
4366 1327 977
AgeI A'CCGG_T
Cuts at: 5501 5501
Size: 6670
AhdI GACnn_n'nnGTC
Cuts at: 5399 5399
Size: 6670
AleI CACnn'nnGTG
Cuts at: 1166 3312 1166
Size: 2146 4524
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6670
AlwNI CAG_nnn'CTG
Cuts at: 2057 2533 3061 4413 5985 2057
Size: 476 528 1352 1572 2742
Fragments arranged by size:
2742 1572 1352 528 476
ApaLI G'TGCA_C
Cuts at: 4727 5883 4727
Size: 1156 5514
ApoI r'AATT_y
Cuts at: 1493 2045 2668 2932 3169 3979 4205 4357 6466
Size: 552 623 264 237 810 226 152 2109
Cuts at: 6466 1493
Size: 1697
Fragments arranged by size:
2109 1697 810 623 552 264 237 226
152
AseI AT'TA_AT
Cuts at: 160 1032 2837 2883 5224 160
Size: 872 1805 46 2341 1606
Fragments arranged by size:
2341 1805 1606 872 46
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6670
AvaI C'yCGr_G
Cuts at: 1870 1943 3995 1870
Size: 73 2052 4545
Fragments arranged by size:
4545 2052 73
BamHI G'GATC_C
Cuts at: 3549 4000 4367 3549
Size: 451 367 5852
Fragments arranged by size:
5852 451 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3141 3559 3991 5446 618
Size: 325 217 627 1354 418 432 1455 1842
Fragments arranged by size:
1842 1455 1354 627 432 418 325 217
BanII G_rGCy'C
Cuts at: 1391 2030 2775 2880 3435 3519 3989 1391
Size: 639 745 105 555 84 470 4072
Fragments arranged by size:
4072 745 639 555 470 105 84
BbeI G_GCGC'C
Cuts at: 3563 3563
Size: 6670
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2127 2175 3229 3748 928
Size: 971 228 48 1054 519 3850
Fragments arranged by size:
3850 1054 971 519 228 48
BbvCI CC'TCA_GC
Cuts at: 3050 3050
Size: 6670
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4861 4895 1903
Size: 34 50 34 2840 34 3678
Fragments arranged by size:
3678 2840 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4562 5772 4562
Size: 1210 5460
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6670
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5281 136
Size: 107 122 71 631 605 3609 1525
Fragments arranged by size:
3609 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6665 6665
Size: 6670
BlpI GC'TnA_GC
Cuts at: 2684 4087 2684
Size: 1403 5267
Bme1580I G_kGCm'C
Cuts at: 1790 3319 4731 5887 1790
Size: 1529 1412 1156 2573
Fragments arranged by size:
2573 1529 1412 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6670
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2045 4401 5359
Size: 416 53 83 399 24 628 2356 958
Cuts at: 5359 442
Size: 1753
Fragments arranged by size:
2356 1753 958 628 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6670
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3629 3629
Size: 6670
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2076 5330 1239
Size: 837 3254 2579
Fragments arranged by size:
3254 2579 837
Bpu10I CC'TnA_GC
Cuts at: 3050 3668 3050
Size: 618 6052
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2789 4795 5660 5958 2789
Size: 2006 865 298 3501
Fragments arranged by size:
3501 2006 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5333 882
Size: 617 3834 2219
Fragments arranged by size:
3834 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2890 4461 493
Size: 711 1686 1571 2702
Fragments arranged by size:
2702 1686 1571 711
BsaBI GATnn'nnATC
Cuts at: 6664 6664
Size: 6670
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3560 4477 4859 5494
Size: 53 83 186 936 2027 917 382 635
Cuts at: 5494 275
Size: 1451
Fragments arranged by size:
2027 1451 936 917 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5103 5501 5775 5922 6333 5103
Size: 398 274 147 411 5440
Fragments arranged by size:
5440 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2014 2044 3506 3536 1178
Size: 30 165 30 611 30 1462 30 4312
Fragments arranged by size:
4312 1462 611 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3057 3480 1186
Size: 1871 423 4376
Fragments arranged by size:
4376 1871 423
BseYI C'CCAG_C
Cuts at: 5873 6369 5873
Size: 496 6174
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3842 3948 1163
Size: 573 2106 106 3885
Fragments arranged by size:
3885 2106 573 106
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4120 4881 5909 1540
Size: 275 186 2119 761 1028 2301
Fragments arranged by size:
2301 2119 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2030 2690 2775 2880 3519 4731 4816 5887 2030
Size: 660 85 105 639 1212 85 1071 2813
Fragments arranged by size:
2813 1212 1071 660 639 105 85 85
BsmI GAATG_Cn'
Cuts at: 3888 4181 4274 3888
Size: 293 93 6284
Fragments arranged by size:
6284 293 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2690 2775 2880 3319 3435 3519
Size: 399 240 660 85 105 439 116 84
Cuts at: 3519 3989 4731 4816 5887 1391
Size: 470 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 660 470 439 399 240
116 105 85 85 84
BspEI T'CCGG_A
Cuts at: 6333 6333
Size: 6670
BspHI T'CATG_A
Cuts at: 2334 2907 3496 4559 6289 2334
Size: 573 589 1063 1730 2715
Fragments arranged by size:
2715 1730 1063 589 573
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3350 4025 844
Size: 2506 675 3489
Fragments arranged by size:
3489 2506 675
BsrBI CCG'CTC
Cuts at: 1394 1823 4557 1394
Size: 429 2734 3507
Fragments arranged by size:
3507 2734 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2678 3586 5165 5339 65
Size: 1183 1430 908 1579 174 1396
Fragments arranged by size:
1579 1430 1396 1183 908 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5314 5501 1700
Size: 110 3504 187 2869
Fragments arranged by size:
3504 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3596 96
Size: 3500 3170
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6670
BssSI C'ACGA_G
Cuts at: 2108 4730 5742 2108
Size: 2622 1012 3036
Fragments arranged by size:
3036 2622 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6670
BstBI TT'CG_AA
Cuts at: 3759 5474 3759
Size: 1715 4955
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6670
BstXI CCAn_nnnn'nTGG
Cuts at: 2016 3028 2016
Size: 1012 5658
BstYI r'GATC_y
Cuts at: 1972 3549 4000 4367 4752 4769 6221 6307 6319
Size: 1577 451 367 385 17 1452 86 12
Cuts at: 6319 6665 1972
Size: 346 1977
Fragments arranged by size:
1977 1577 1452 451 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3126 513
Size: 552 2061 4057
Fragments arranged by size:
4057 2061 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3139 487
Size: 683 56 1913 4018
Fragments arranged by size:
4018 1913 683 56
BtsI GCAGTG_nn'
Cuts at: 4186 4980 5000 4186
Size: 794 20 5856
Fragments arranged by size:
5856 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6670
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6635
DraI TTT'AAA
Cuts at: 3285 3974 3285
Size: 689 5981
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5677 809
Size: 507 4361 1802
Fragments arranged by size:
4361 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3442 4117 5006
Size: 54 1425 50 275 18 1612 675 889
Cuts at: 5006 6635 8
Size: 1629 43
Fragments arranged by size:
1629 1612 1425 889 675 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4117 1537
Size: 275 2305 4090
Fragments arranged by size:
4090 2305 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2109 2802 4600 1353
Size: 756 693 1798 3423
Fragments arranged by size:
3423 1798 756 693
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3204 5257 5631 5777 387
Size: 1064 138 1615 2053 374 146 1280
Fragments arranged by size:
2053 1615 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2028 2773 2878 3517 2028
Size: 745 105 639 5181
Fragments arranged by size:
5181 745 639 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2076 3223 3622 4733 5330 6117 6385
Size: 393 444 1147 399 1111 597 787 268
Cuts at: 6385 1239
Size: 1524
Fragments arranged by size:
1524 1147 1111 787 597 444 399 393
268
EcoO109I rG'GnC_Cy
Cuts at: 2195 2195
Size: 6670
EcoRI G'AATT_C
Cuts at: 3979 4357 3979
Size: 378 6292
EcoRV GAT'ATC
Cuts at: 3329 3329
Size: 6670
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2291 3316 2291
Size: 1025 5645
FspI TGC'GCA
Cuts at: 3083 5176 3083
Size: 2093 4577
HaeII r_GCGC'y
Cuts at: 1678 3563 5817 1678
Size: 1885 2254 2531
Fragments arranged by size:
2531 2254 1885
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3098 3130 5311 5343 5385
Size: 32 135 32 1526 32 2181 32 42
Cuts at: 5385 5417 1373
Size: 32 2626
Fragments arranged by size:
2626 2181 1526 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2079 2650 4014 4260 669
Size: 988 281 141 571 1364 246 3079
Fragments arranged by size:
3079 1364 988 571 281 246 141
HindIII A'AGCT_T
Cuts at: 748 2471 748
Size: 1723 4947
HpaI GTT'AAC
Cuts at: 2650 4260 2650
Size: 1610 5060
KasI G'GCGC_C
Cuts at: 3559 3559
Size: 6670
KpnI G_GTAC'C
Cuts at: 3145 3995 3145
Size: 850 5820
MfeI C'AATT_G
Cuts at: 3711 4269 3711
Size: 558 6112
MluI A'CGCG_T
Cuts at: 6546 6546
Size: 6670
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2581 5784 5968 1305
Size: 1276 3203 184 2007
Fragments arranged by size:
3203 2007 1276 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3444 10
Size: 54 1425 343 1612 3236
Fragments arranged by size:
3236 1612 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2014 3312 4628 4987 5146 518
Size: 648 848 1298 1316 359 159 2042
Fragments arranged by size:
2042 1316 1298 848 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3061 4086 4763 5911 6156 1606
Size: 1455 1025 677 1148 245 2120
Fragments arranged by size:
2120 1455 1148 1025 677 245
NarI GG'CG_CC
Cuts at: 3560 3560
Size: 6670
NcoI C'CATG_G
Cuts at: 513 1065 3126 513
Size: 552 2061 4057
Fragments arranged by size:
4057 2061 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6670
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6670
NotI GC'GGCC_GC
Cuts at: 4117 4117
Size: 6670
NspI r_CATG'y
Cuts at: 1132 1229 3088 3595 4028 5573 1132
Size: 97 1859 507 433 1545 2229
Fragments arranged by size:
2229 1859 1545 507 433 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6670
PciI A'CATG_T
Cuts at: 5569 5569
Size: 6670
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6670
PmeI GTTT'AAAC
Cuts at: 3974 3974
Size: 6670
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2757 2789 4739 4771 6252 6284 2757
Size: 32 1950 32 1481 32 3143
Fragments arranged by size:
3143 1950 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2195 2195
Size: 6670
PshAI GACnn'nnGTC
Cuts at: 1613 3572 1613
Size: 1959 4711
PsiI TTA'TAA
Cuts at: 4240 4240
Size: 6670
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2905 2937 2905
Size: 32 6638
PstI C_TGCA'G
Cuts at: 830 4022 830
Size: 3192 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6670
PvuII CAG'CTG
Cuts at: 3061 3061
Size: 6670
SacI G_AGCT'C
Cuts at: 2030 2775 2880 3519 2030
Size: 745 105 639 5181
Fragments arranged by size:
5181 745 639 105
SalI G'TCGA_C
Cuts at: 1936 4012 1936
Size: 2076 4594
SapI GCTCTTCn'nnn_
Cuts at: 2802 2802
Size: 6670
SbfI CC_TGCA'GG
Cuts at: 4022 4022
Size: 6670
ScaI AGT'ACT
Cuts at: 4918 6630 4918
Size: 1712 4958
SfcI C'TryA_G
Cuts at: 826 1045 2525 4018 5153 5834 6025 826
Size: 219 1480 1493 1135 681 191 1471
Fragments arranged by size:
1493 1480 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 3561 3561
Size: 6670
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6670
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6670
SmaI CCC'GGG
Cuts at: 1872 3997 1872
Size: 2125 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2750 2768 4774 5675 5937 820
Size: 197 926 807 18 2006 901 262 1553
Fragments arranged by size:
2006 1553 926 901 807 262 197 18
SnaBI TAC'GTA
Cuts at: 493 4461 493
Size: 3968 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6670
SphI G_CATG'C
Cuts at: 1132 4028 1132
Size: 2896 3774
SspI AAT'ATT
Cuts at: 5 52 4594 5
Size: 47 4542 2081
Fragments arranged by size:
4542 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6670
StyI C'CwwG_G
Cuts at: 513 1065 2143 2187 3126 3137 3880 4109 513
Size: 552 1078 44 939 11 743 229 3074
Fragments arranged by size:
3074 1078 939 743 552 229 44 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2423 4697 4714 4867 5052 5497 325
Size: 1255 843 2274 17 153 185 445 1498
Fragments arranged by size:
2274 1498 1255 843 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2759 3596 4916
Size: 274 80 33 51 1443 782 837 1320
Cuts at: 4916 6628 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1320 837 782 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3506 3538 3898 3930 6630 6662 3506
Size: 32 360 32 2700 32 3514
Fragments arranged by size:
3514 2700 360 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6670
XbaI T'CTAG_A
Cuts at: 3944 4006 3944
Size: 62 6608
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6670
XmaI C'CCGG_G
Cuts at: 1870 3995 1870
Size: 2125 4545
XmnI GAAnn'nnTTC
Cuts at: 4799 4799
Size: 6670
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4478 276
Size: 53 83 186 936 2944 2468
Fragments arranged by size:
2944 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI