Restriction map for
pFN21AE3684
(Circular) (Six-base) MAPSORT of: pFN21AE3684.seq Check: 8422 from: 1 to: 6172
pFN21AE3684 6172 bp
With 182 enzymes: SgfI *
January 27, 2015 16:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3982 278
Size: 53 83 186 936 2446 2468
Fragments arranged by size:
2468 2446 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3515 1937
Size: 1578 4594
Acc65I G'GTAC_C
Cuts at: 3402 3493 3402
Size: 91 6081
AclI AA'CG_TT
Cuts at: 1563 4299 4672 1563
Size: 2736 373 3063
Fragments arranged by size:
3063 2736 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2330 2573 4235 5619 5887 1632
Size: 698 243 1662 1384 268 1917
Fragments arranged by size:
1917 1662 1384 698 268 243
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5975
AflIII A'CryG_T
Cuts at: 1203 5071 6048 1203
Size: 3868 977 1327
Fragments arranged by size:
3868 1327 977
AgeI A'CCGG_T
Cuts at: 3399 5003 3399
Size: 1604 4568
AhdI GACnn_n'nnGTC
Cuts at: 4901 4901
Size: 6172
AleI CACnn'nnGTG
Cuts at: 1166 3029 1166
Size: 1863 4309
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6172
AlwNI CAG_nnn'CTG
Cuts at: 3052 3915 5487 3052
Size: 863 1572 3737
Fragments arranged by size:
3737 1572 863
ApaI G_GGCC'C
Cuts at: 2339 2689 2339
Size: 350 5822
ApaLI G'TGCA_C
Cuts at: 4229 5385 4229
Size: 1156 5016
ApoI r'AATT_y
Cuts at: 1493 2938 3481 3707 3859 5968 1493
Size: 1445 543 226 152 2109 1697
Fragments arranged by size:
2109 1697 1445 543 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4726 160
Size: 872 3694 1606
Fragments arranged by size:
3694 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6172
AvaI C'yCGr_G
Cuts at: 1870 1943 2718 2775 2925 3497 1870
Size: 73 775 57 150 572 4545
Fragments arranged by size:
4545 775 572 150 73 57
BamHI G'GATC_C
Cuts at: 2171 3502 3869 2171
Size: 1331 367 4474
Fragments arranged by size:
4474 1331 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2091 3402 3493 4948 618
Size: 325 217 627 304 1311 91 1455 1842
Fragments arranged by size:
1842 1455 1311 627 325 304 217 91
BanII G_rGCy'C
Cuts at: 1391 2078 2300 2339 2483 2504 2689 3491 1391
Size: 687 222 39 144 21 185 802 4072
Fragments arranged by size:
4072 802 687 222 185 144 39 21
BbeI G_GCGC'C
Cuts at: 2095 2095
Size: 6172
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5201
BbvCI CC'TCA_GC
Cuts at: 2954 2954
Size: 6172
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2362 2396 4363 4397 1903
Size: 34 50 34 341 34 1967 34 3678
Fragments arranged by size:
3678 1967 341 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2817 4064 5274 2817
Size: 1247 1210 3715
Fragments arranged by size:
3715 1247 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6172
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3422 4783 136
Size: 107 122 71 631 605 1750 1361 1525
Fragments arranged by size:
1750 1525 1361 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6167 6167
Size: 6172
BlpI GC'TnA_GC
Cuts at: 3589 3589
Size: 6172
Bme1580I G_kGCm'C
Cuts at: 1790 2339 2689 4233 5389 1790
Size: 549 350 1544 1156 2573
Fragments arranged by size:
2573 1544 1156 549 350
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6172
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3903 4861 442
Size: 416 53 83 399 24 2486 958 1753
Fragments arranged by size:
2486 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6172
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2314 2314
Size: 6172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2388 3230 3427 4832 1239
Size: 1149 842 197 1405 2579
Fragments arranged by size:
2579 1405 1149 842 197
Bpu10I CC'TnA_GC
Cuts at: 2072 2126 2954 2072
Size: 54 828 5290
Fragments arranged by size:
5290 828 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4297 5162 5460 4297
Size: 865 298 5009
Fragments arranged by size:
5009 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2340 4835 882
Size: 617 841 2495 2219
Fragments arranged by size:
2495 2219 841 617
BsaAI yAC'GTr
Cuts at: 493 1204 3963 493
Size: 711 2759 2702
Fragments arranged by size:
2759 2702 711
BsaBI GATnn'nnATC
Cuts at: 6166 6166
Size: 6172
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2092 3381 3979 4361
Size: 53 83 186 936 559 1289 598 382
Cuts at: 4361 4996 275
Size: 635 1451
Fragments arranged by size:
1451 1289 936 635 598 559 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3399 4605 5003 5277 5424 5835 3399
Size: 1206 398 274 147 411 3736
Fragments arranged by size:
3736 1206 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2885 2915 3248 3278 1178
Size: 30 165 30 1482 30 333 30 4072
Fragments arranged by size:
4072 1482 333 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2210 2213 2216 2225 2234 2249 2332 2612
Size: 1024 3 3 9 9 15 83 280
Cuts at: 2612 2615 2618 2693 2943 1186
Size: 3 3 75 250 4415
Fragments arranged by size:
4415 1024 280 250 83 75 15 9
9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2482 2503 3082 5375 5871 2482
Size: 21 579 2293 496 2783
Fragments arranged by size:
2783 2293 579 496 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2807 3212 1163
Size: 573 1071 405 4123
Fragments arranged by size:
4123 1071 573 405
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3367 3622 4383 5411 1540
Size: 275 186 1366 255 761 1028 2301
Fragments arranged by size:
2301 1366 1028 761 275 255 186
BsiHKAI G_wGCw'C
Cuts at: 2300 2733 4233 4318 5389 2300
Size: 433 1500 85 1071 3083
Fragments arranged by size:
3083 1500 1071 433 85
BsmI GAATG_Cn'
Cuts at: 3683 3776 3683
Size: 93 6079
BsmBI CGTCTCn'nnnn_
Cuts at: 2697 2697
Size: 6172
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2078 2300 2339 2483 2504 2689 2733
Size: 399 288 222 39 144 21 185 44
Cuts at: 2733 3491 4233 4318 5389 1391
Size: 758 742 85 1071 2174
Fragments arranged by size:
2174 1071 758 742 399 288 222 185
144 85 44 39 21
BspEI T'CCGG_A
Cuts at: 5835 5835
Size: 6172
BspHI T'CATG_A
Cuts at: 4061 5791 4061
Size: 1730 4442
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3527 844
Size: 2683 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4059 1394
Size: 429 2236 3507
Fragments arranged by size:
3507 2236 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4667 4841 65
Size: 1183 3419 174 1396
Fragments arranged by size:
3419 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2766 3399 4816 5003 1700
Size: 110 956 633 1417 187 2869
Fragments arranged by size:
2869 1417 956 633 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6172
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6172
BssSI C'ACGA_G
Cuts at: 4232 5244 4232
Size: 1012 5160
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6172
BstBI TT'CG_AA
Cuts at: 4976 4976
Size: 6172
BstEII G'GTnAC_C
Cuts at: 1184 3292 1184
Size: 2108 4064
BstXI CCAn_nnnn'nTGG
Cuts at: 2518 2968 3082 3246 2518
Size: 450 114 164 5444
Fragments arranged by size:
5444 450 164 114
BstYI r'GATC_y
Cuts at: 1972 2171 3502 3869 4254 4271 5723 5809 5821
Size: 199 1331 367 385 17 1452 86 12
Cuts at: 5821 6167 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1331 385 367 346 199 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2473 2473
Size: 6172
BtgI C'CryG_G
Cuts at: 513 1065 2087 2166 2700 3091 513
Size: 552 1022 79 534 391 3594
Fragments arranged by size:
3594 1022 552 534 391 79
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3418 487
Size: 683 56 2192 3241
Fragments arranged by size:
3241 2192 683 56
BtsI GCAGTG_nn'
Cuts at: 3688 4482 4502 3688
Size: 794 20 5358
Fragments arranged by size:
5358 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6172
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6137
DraI TTT'AAA
Cuts at: 3476 3476
Size: 6172
DraIII CAC_nnn'GTG
Cuts at: 3014 3014
Size: 6172
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5179 809
Size: 507 3863 1802
Fragments arranged by size:
3863 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3364 3619 4508
Size: 54 1425 50 275 18 1534 255 889
Cuts at: 4508 6137 8
Size: 1629 43
Fragments arranged by size:
1629 1534 1425 889 275 255 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3364 3619 1537
Size: 275 1552 255 4090
Fragments arranged by size:
4090 1552 275 255
EarI CTCTTCn'nnn_
Cuts at: 1353 2129 2202 2226 2288 2492 2589 4102 1353
Size: 776 73 24 62 204 97 1513 3423
Fragments arranged by size:
3423 1513 776 204 97 73 62 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3389 4759 5133 5279 387
Size: 1064 138 1800 1370 374 146 1280
Fragments arranged by size:
1800 1370 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2298 2298
Size: 6172
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2330 2388 2573 3230 3427 4235 4832
Size: 393 698 58 185 657 197 808 597
Cuts at: 4832 5619 5887 1239
Size: 787 268 1524
Fragments arranged by size:
1524 808 787 698 657 597 393 268
197 185 58
EcoNI CCTnn'n_nnAGG
Cuts at: 2030 3044 2030
Size: 1014 5158
EcoO109I rG'GnC_Cy
Cuts at: 2083 2146 2335 2467 2685 2686 3148 2083
Size: 63 189 132 218 1 462 5107
Fragments arranged by size:
5107 462 218 189 132 63 1
EcoRI G'AATT_C
Cuts at: 3481 3859 3481
Size: 378 5794
FspI TGC'GCA
Cuts at: 4678 4678
Size: 6172
HaeII r_GCGC'y
Cuts at: 1678 2095 5319 1678
Size: 417 3224 2531
Fragments arranged by size:
3224 2531 417
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3248 3280 4813 4845 4887
Size: 32 135 32 1676 32 1533 32 42
Cuts at: 4887 4919 1373
Size: 32 2626
Fragments arranged by size:
2626 1676 1533 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3516 3762 669
Size: 988 281 1578 246 3079
Fragments arranged by size:
3079 1578 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6172
HpaI GTT'AAC
Cuts at: 3762 3762
Size: 6172
KasI G'GCGC_C
Cuts at: 2091 2091
Size: 6172
KpnI G_GTAC'C
Cuts at: 3406 3497 3406
Size: 91 6081
MfeI C'AATT_G
Cuts at: 3771 3771
Size: 6172
MluI A'CGCG_T
Cuts at: 6048 6048
Size: 6172
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2985 5286 5470 1305
Size: 1680 2301 184 2007
Fragments arranged by size:
2301 2007 1680 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4350
Fragments arranged by size:
4350 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3029 3333 4130 4489 4648 518
Size: 648 1863 304 797 359 159 2042
Fragments arranged by size:
2042 1863 797 648 359 304 159
MspA1I CmG'CkG
Cuts at: 1606 2089 3059 3409 3588 4265 5413 5658 1606
Size: 483 970 350 179 677 1148 245 2120
Fragments arranged by size:
2120 1148 970 677 483 350 245 179
NaeI GCC'GGC
Cuts at: 2768 2768
Size: 6172
NarI GG'CG_CC
Cuts at: 2092 2092
Size: 6172
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5620
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6172
NgoMIV G'CCGG_C
Cuts at: 2766 2766
Size: 6172
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6172
NotI GC'GGCC_GC
Cuts at: 3619 3619
Size: 6172
NspI r_CATG'y
Cuts at: 1132 1229 2806 3530 5075 1132
Size: 97 1577 724 1545 2229
Fragments arranged by size:
2229 1577 1545 724 97
PasI CC'CwG_GG
Cuts at: 1348 2332 2746 1348
Size: 984 414 4774
Fragments arranged by size:
4774 984 414
PciI A'CATG_T
Cuts at: 5071 5071
Size: 6172
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3014 1108
Size: 1906 4266
PmeI GTTT'AAAC
Cuts at: 3476 3476
Size: 6172
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4241 4273 5754 5786 4241
Size: 32 1481 32 4627
Fragments arranged by size:
4627 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2467 3148 2467
Size: 681 5491
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6172
PsiI TTA'TAA
Cuts at: 3742 3742
Size: 6172
PspOMI G'GGCC_C
Cuts at: 2335 2685 2335
Size: 350 5822
PstI C_TGCA'G
Cuts at: 830 3360 3423 3524 830
Size: 2530 63 101 3478
Fragments arranged by size:
3478 2530 101 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6172
PvuII CAG'CTG
Cuts at: 3059 3059
Size: 6172
SacI G_AGCT'C
Cuts at: 2300 2300
Size: 6172
SacII CC_GC'GG
Cuts at: 2090 2090
Size: 6172
SalI G'TCGA_C
Cuts at: 1936 3514 1936
Size: 1578 4594
SanDI GG'GwC_CC
Cuts at: 3148 3148
Size: 6172
SapI GCTCTTCn'nnn_
Cuts at: 2288 2492 2288
Size: 204 5968
SbfI CC_TGCA'GG
Cuts at: 3423 3524 3423
Size: 101 6071
ScaI AGT'ACT
Cuts at: 4420 6132 4420
Size: 1712 4460
SfcI C'TryA_G
Cuts at: 826 1045 3356 3419 3520 4655 5336 5527 826
Size: 219 2311 63 101 1135 681 191 1471
Fragments arranged by size:
2311 1471 1135 681 219 191 101 63
SfoI GGC'GCC
Cuts at: 2093 2093
Size: 6172
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6172
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6172
SmaI CCC'GGG
Cuts at: 1872 2720 2777 3499 1872
Size: 848 57 722 4545
Fragments arranged by size:
4545 848 722 57
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4276 5177 5439 820
Size: 197 926 2333 901 262 1553
Fragments arranged by size:
2333 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3963 493
Size: 3470 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6172
SphI G_CATG'C
Cuts at: 1132 3530 1132
Size: 2398 3774
SspI AAT'ATT
Cuts at: 5 52 4096 5
Size: 47 4044 2081
Fragments arranged by size:
4044 2081 47
StuI AGG'CCT
Cuts at: 1517 3302 3425 1517
Size: 1785 123 4264
Fragments arranged by size:
4264 1785 123
StyI C'CwwG_G
Cuts at: 513 1065 2325 2339 3283 3611 513
Size: 552 1260 14 944 328 3074
Fragments arranged by size:
3074 1260 944 552 328 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3311 4199 4216 4369 4554 4999 325
Size: 1255 1731 888 17 153 185 445 1498
Fragments arranged by size:
1731 1498 1255 888 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4418 6130 96
Size: 274 80 33 51 1443 2441 1712 138
Fragments arranged by size:
2441 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6132 6164 6132
Size: 32 6140
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6172
XbaI T'CTAG_A
Cuts at: 3508 3508
Size: 6172
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6172
XmaI C'CCGG_G
Cuts at: 1870 2718 2775 3497 1870
Size: 848 57 722 4545
Fragments arranged by size:
4545 848 722 57
XmnI GAAnn'nnTTC
Cuts at: 2846 2978 4301 2846
Size: 132 1323 4717
Fragments arranged by size:
4717 1323 132
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3980 276
Size: 53 83 186 936 2446 2468
Fragments arranged by size:
2468 2446 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SexAI SfiI SrfI
SwaI XcmI