Restriction map for
pFN21AE3681
(Circular) (Six-base) MAPSORT of: pFN21AE3681.seq Check: 7718 from: 1 to: 5794
pFN21AE3681 5794 bp
With 182 enzymes: SgfI *
July 6, 2012 17:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2980 2980
Size: 5794
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2678 3604 278
Size: 53 83 186 936 1142 926 2468
Fragments arranged by size:
2468 1142 936 926 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3137 1937
Size: 1200 4594
Acc65I G'GTAC_C
Cuts at: 3115 3115
Size: 5794
AclI AA'CG_TT
Cuts at: 1563 3921 4294 1563
Size: 2358 373 3063
Fragments arranged by size:
3063 2358 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2359 3857 5241 5509 1632
Size: 727 1498 1384 268 1917
Fragments arranged by size:
1917 1498 1384 727 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5597
AflIII A'CryG_T
Cuts at: 1203 4693 5670 1203
Size: 3490 977 1327
Fragments arranged by size:
3490 1327 977
AgeI A'CCGG_T
Cuts at: 4625 4625
Size: 5794
AhdI GACnn_n'nnGTC
Cuts at: 4523 4523
Size: 5794
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5794
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5794
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2198 2230 2198
Size: 32 5762
AlwNI CAG_nnn'CTG
Cuts at: 2430 2749 2758 3537 5109 2430
Size: 319 9 779 1572 3115
Fragments arranged by size:
3115 1572 779 319 9
ApaLI G'TGCA_C
Cuts at: 2993 3851 5007 2993
Size: 858 1156 3780
Fragments arranged by size:
3780 1156 858
ApoI r'AATT_y
Cuts at: 1493 3103 3329 3481 5590 1493
Size: 1610 226 152 2109 1697
Fragments arranged by size:
2109 1697 1610 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4348 160
Size: 872 3316 1606
Fragments arranged by size:
3316 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5794
AvaI C'yCGr_G
Cuts at: 1870 1943 2228 2288 2860 2930 3119 1870
Size: 73 285 60 572 70 189 4545
Fragments arranged by size:
4545 572 285 189 73 70 60
BamHI G'GATC_C
Cuts at: 3124 3491 3124
Size: 367 5427
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2283 2866 3115 4570 618
Size: 325 217 627 496 583 249 1455 1842
Fragments arranged by size:
1842 1455 627 583 496 325 249 217
BanII G_rGCy'C
Cuts at: 1391 2107 2446 3113 1391
Size: 716 339 667 4072
Fragments arranged by size:
4072 716 667 339
BbeI G_GCGC'C
Cuts at: 2870 2870
Size: 5794
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4823
BbvCI CC'TCA_GC
Cuts at: 2315 2777 2315
Size: 462 5332
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2613 2647 3985 4019 1903
Size: 34 676 34 1338 34 3678
Fragments arranged by size:
3678 1338 676 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3686 4896 3686
Size: 1210 4584
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5794
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4405 136
Size: 107 122 71 631 605 2733 1525
Fragments arranged by size:
2733 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2352 2415 5789 2352
Size: 63 3374 2357
Fragments arranged by size:
3374 2357 63
BlpI GC'TnA_GC
Cuts at: 3211 3211
Size: 5794
Bme1580I G_kGCm'C
Cuts at: 1790 2401 2631 2997 3855 5011 1790
Size: 611 230 366 858 1156 2573
Fragments arranged by size:
2573 1156 858 611 366 230
BmgBI CAC'GTC
Cuts at: 1318 2310 1318
Size: 992 4802
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2440 2843 3525
Size: 416 53 83 399 24 1023 403 682
Cuts at: 3525 4483 442
Size: 958 1753
Fragments arranged by size:
1753 1023 958 682 416 403 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5794
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2200 4454 1239
Size: 961 2254 2579
Fragments arranged by size:
2579 2254 961
Bpu10I CC'TnA_GC
Cuts at: 2315 2777 2315
Size: 462 5332
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2237 2564 3919 4784 5082 2237
Size: 327 1355 865 298 2949
Fragments arranged by size:
2949 1355 865 327 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4457 882
Size: 617 2958 2219
Fragments arranged by size:
2958 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2278 3585 493
Size: 711 1074 1307 2702
Fragments arranged by size:
2702 1307 1074 711
BsaBI GATnn'nnATC
Cuts at: 5788 5788
Size: 5794
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2085 2675 2867 3601
Size: 53 83 186 936 552 590 192 734
Cuts at: 3601 3983 4618 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 734 635 590 552 382 192
186 83 53
BsaWI w'CCGG_w
Cuts at: 4227 4625 4899 5046 5457 4227
Size: 398 274 147 411 4564
Fragments arranged by size:
4564 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2198 2228 1178
Size: 30 165 30 795 30 4744
Fragments arranged by size:
4744 795 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2173 2528 2546 1186
Size: 987 355 18 4434
Fragments arranged by size:
4434 987 355 18
BseYI C'CCAG_C
Cuts at: 2010 2144 4997 5493 2010
Size: 134 2853 496 2311
Fragments arranged by size:
2853 2311 496 134
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2939 1163
Size: 573 1203 4018
Fragments arranged by size:
4018 1203 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2410 3244 4005 5033 1540
Size: 275 186 409 834 761 1028 2301
Fragments arranged by size:
2301 1028 834 761 409 275 186
BsiHKAI G_wGCw'C
Cuts at: 2107 2297 2446 2544 2997 3855 3940 5011 2107
Size: 190 149 98 453 858 85 1071 2890
Fragments arranged by size:
2890 1071 858 453 190 149 98 85
BsmI GAATG_Cn'
Cuts at: 3305 3398 3305
Size: 93 5701
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2107 2297 2401 2446 2544 2631 2997
Size: 399 317 190 104 45 98 87 366
Cuts at: 2997 3113 3855 3940 5011 1391
Size: 116 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 366 317 190 116
104 98 87 85 45
BspEI T'CCGG_A
Cuts at: 5457 5457
Size: 5794
BspHI T'CATG_A
Cuts at: 3683 5413 3683
Size: 1730 4064
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2689 2980 3149 844
Size: 1845 291 169 3489
Fragments arranged by size:
3489 1845 291 169
BsrBI CCG'CTC
Cuts at: 1394 1823 2935 3055 3681 1394
Size: 429 1112 120 626 3507
Fragments arranged by size:
3507 1112 626 429 120
BsrDI GCAATG_nn'
Cuts at: 65 1248 4289 4463 65
Size: 1183 3041 174 1396
Fragments arranged by size:
3041 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2656 3000 4438 4625 1700
Size: 110 846 344 1438 187 2869
Fragments arranged by size:
2869 1438 846 344 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5794
BssHII G'CGCG_C
Cuts at: 1925 2665 2667 2939 1925
Size: 740 2 272 4780
Fragments arranged by size:
4780 740 272 2
BssSI C'ACGA_G
Cuts at: 2485 3854 4866 2485
Size: 1369 1012 3413
Fragments arranged by size:
3413 1369 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2704 794
Size: 1910 3884
BstBI TT'CG_AA
Cuts at: 4598 4598
Size: 5794
BstEII G'GTnAC_C
Cuts at: 1184 2947 1184
Size: 1763 4031
BstYI r'GATC_y
Cuts at: 1972 2232 2352 2415 3124 3491 3876 3893 5345
Size: 260 120 63 709 367 385 17 1452
Cuts at: 5345 5431 5443 5789 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 709 385 367 346 260 120
86 63 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2070 2685 513
Size: 552 939 66 615 3622
Fragments arranged by size:
3622 939 615 552 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5055
Fragments arranged by size:
5055 683 56
BtsI GCAGTG_nn'
Cuts at: 2631 2995 3310 4104 4124 2631
Size: 364 315 794 20 4301
Fragments arranged by size:
4301 794 364 315 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5794
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5759
DraI TTT'AAA
Cuts at: 3098 3098
Size: 5794
DraIII CAC_nnn'GTG
Cuts at: 2851 2851
Size: 5794
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4801 809
Size: 507 3485 1802
Fragments arranged by size:
3485 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2407 2658 3241
Size: 54 1425 50 275 18 577 251 583
Cuts at: 3241 4130 5759 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 583 577 275 251 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2407 3241 1537
Size: 275 595 834 4090
Fragments arranged by size:
4090 834 595 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3724 1353
Size: 2371 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2777 2878 4381 4755 4901 387
Size: 1064 138 1188 101 1503 374 146 1280
Fragments arranged by size:
1503 1280 1188 1064 374 146 138 101
EcoICRI GAG'CTC
Cuts at: 2105 2444 2105
Size: 339 5455
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2200 2359 3857 4454 5241 5509 1239
Size: 393 568 159 1498 597 787 268 1524
Fragments arranged by size:
1524 1498 787 597 568 393 268 159
EcoNI CCTnn'n_nnAGG
Cuts at: 2528 2528
Size: 5794
EcoO109I rG'GnC_Cy
Cuts at: 2092 2092
Size: 5794
EcoRI G'AATT_C
Cuts at: 3103 3481 3103
Size: 378 5416
EcoRV GAT'ATC
Cuts at: 2246 2246
Size: 5794
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2371 2371
Size: 5794
FspI TGC'GCA
Cuts at: 4300 4300
Size: 5794
HaeII r_GCGC'y
Cuts at: 1678 2497 2870 4941 1678
Size: 819 373 2071 2531
Fragments arranged by size:
2531 2071 819 373
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2235 2267 4435 4467 4509
Size: 32 135 32 663 32 2168 32 42
Cuts at: 4509 4541 1373
Size: 32 2626
Fragments arranged by size:
2626 2168 663 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2260 2648 3138 3384 669
Size: 988 281 322 388 490 246 3079
Fragments arranged by size:
3079 988 490 388 322 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5794
HpaI GTT'AAC
Cuts at: 3384 3384
Size: 5794
KasI G'GCGC_C
Cuts at: 2866 2866
Size: 5794
KpnI G_GTAC'C
Cuts at: 3119 3119
Size: 5794
MfeI C'AATT_G
Cuts at: 3393 3393
Size: 5794
MluI A'CGCG_T
Cuts at: 5670 5670
Size: 5794
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2595 2922 3084 4908 5092 1305
Size: 1290 327 162 1824 184 2007
Fragments arranged by size:
2007 1824 1290 327 184 162
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3972
Fragments arranged by size:
3972 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2795 3752 4111 4270 518
Size: 648 1629 957 359 159 2042
Fragments arranged by size:
2042 1629 957 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2043 2220 2331 2500 2687 2695 3210 3887
Size: 437 177 111 169 187 8 515 677
Cuts at: 3887 5035 5280 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 515 437 245 187 177
169 111 8
NaeI GCC'GGC
Cuts at: 2658 3002 2658
Size: 344 5450
NarI GG'CG_CC
Cuts at: 2867 2867
Size: 5794
NcoI C'CATG_G
Cuts at: 513 1065 2004 2070 513
Size: 552 939 66 4237
Fragments arranged by size:
4237 939 552 66
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5794
NgoMIV G'CCGG_C
Cuts at: 2656 3000 2656
Size: 344 5450
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5794
NotI GC'GGCC_GC
Cuts at: 3241 3241
Size: 5794
NspI r_CATG'y
Cuts at: 1132 1229 3152 4697 1132
Size: 97 1923 1545 2229
Fragments arranged by size:
2229 1923 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5794
PciI A'CATG_T
Cuts at: 4693 4693
Size: 5794
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2430 1108
Size: 1322 4472
PfoI T'CCnGG_A
Cuts at: 2235 2235
Size: 5794
PmeI GTTT'AAAC
Cuts at: 3098 3098
Size: 5794
PmlI CAC'GTG
Cuts at: 2278 2278
Size: 5794
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2198 2230 3863 3895 5376 5408 2198
Size: 32 1633 32 1481 32 2584
Fragments arranged by size:
2584 1633 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2121 1613
Size: 508 5286
PsiI TTA'TAA
Cuts at: 3364 3364
Size: 5794
PspXI vC'TCGA_Gb
Cuts at: 2288 2288
Size: 5794
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2318 2350 2318
Size: 32 5762
PstI C_TGCA'G
Cuts at: 830 2200 2700 2754 3146 830
Size: 1370 500 54 392 3478
Fragments arranged by size:
3478 1370 500 392 54
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5794
PvuII CAG'CTG
Cuts at: 2220 2331 2500 2695 2220
Size: 111 169 195 5319
Fragments arranged by size:
5319 195 169 111
SacI G_AGCT'C
Cuts at: 2107 2446 2107
Size: 339 5455
SacII CC_GC'GG
Cuts at: 2688 2688
Size: 5794
SalI G'TCGA_C
Cuts at: 1936 3136 1936
Size: 1200 4594
SbfI CC_TGCA'GG
Cuts at: 3146 3146
Size: 5794
ScaI AGT'ACT
Cuts at: 4042 5754 4042
Size: 1712 4082
SfcI C'TryA_G
Cuts at: 826 1045 2196 2696 2750 3142 4277 4958 5149
Size: 219 1151 500 54 392 1135 681 191
Cuts at: 5149 826
Size: 1471
Fragments arranged by size:
1471 1151 1135 681 500 392 219 191
54
SfoI GGC'GCC
Cuts at: 2868 2868
Size: 5794
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5794
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5794
SmaI CCC'GGG
Cuts at: 1872 3121 1872
Size: 1249 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2228 2252 2288 2579 3898 4799
Size: 197 926 285 24 36 291 1319 901
Cuts at: 4799 5061 820
Size: 262 1553
Fragments arranged by size:
1553 1319 926 901 291 285 262 197
36 24
SnaBI TAC'GTA
Cuts at: 493 3585 493
Size: 3092 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5794
SphI G_CATG'C
Cuts at: 1132 3152 1132
Size: 2020 3774
SspI AAT'ATT
Cuts at: 5 52 3718 5
Size: 47 3666 2081
Fragments arranged by size:
3666 2081 47
StuI AGG'CCT
Cuts at: 1517 2129 1517
Size: 612 5182
StyI C'CwwG_G
Cuts at: 513 1065 2004 2070 3233 513
Size: 552 939 66 1163 3074
Fragments arranged by size:
3074 1163 939 552 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2256 2422 2862 3821 3838 3991 4176
Size: 1255 676 166 440 959 17 153 185
Cuts at: 4176 4621 325
Size: 445 1498
Fragments arranged by size:
1498 1255 959 676 445 440 185 166
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4040 5752 96
Size: 274 80 33 51 1443 2063 1712 138
Fragments arranged by size:
2063 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2380 2412 3009 3041 5754 5786 2380
Size: 32 597 32 2713 32 2388
Fragments arranged by size:
2713 2388 597 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5794
XbaI T'CTAG_A
Cuts at: 3130 3130
Size: 5794
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2504 2504
Size: 5794
XhoI C'TCGA_G
Cuts at: 1943 2228 2288 1943
Size: 285 60 5449
Fragments arranged by size:
5449 285 60
XmaI C'CCGG_G
Cuts at: 1870 3119 1870
Size: 1249 4545
XmnI GAAnn'nnTTC
Cuts at: 3923 3923
Size: 5794
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2676 3602 276
Size: 53 83 186 936 1142 926 2468
Fragments arranged by size:
2468 1142 936 926 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PstI PvuI PvuII SacI
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I FseI FspAI NruI NsiI
PacI PpuMI PspOMI RsrII SanDI SapI SexAI SfiI
SrfI SwaI