Restriction map for
pFN21AE3679
(Circular) (Six-base) MAPSORT of: pFN21AE3679.seq Check: 9122 from: 1 to: 5617
pFN21AE3679 5617 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3427 278
Size: 53 83 186 936 1891 2468
Fragments arranged by size:
2468 1891 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2960 1937
Size: 1023 4594
Acc65I G'GTAC_C
Cuts at: 2938 2938
Size: 5617
AclI AA'CG_TT
Cuts at: 1563 3744 4117 1563
Size: 2181 373 3063
Fragments arranged by size:
3063 2181 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3680 5064 5332 1632
Size: 2048 1384 268 1917
Fragments arranged by size:
2048 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5420
AflIII A'CryG_T
Cuts at: 1203 2795 4516 5493 1203
Size: 1592 1721 977 1327
Fragments arranged by size:
1721 1592 1327 977
AgeI A'CCGG_T
Cuts at: 2058 4448 2058
Size: 2390 3227
AhdI GACnn_n'nnGTC
Cuts at: 4346 4346
Size: 5617
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5617
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5617
AlwNI CAG_nnn'CTG
Cuts at: 3360 4932 3360
Size: 1572 4045
ApaLI G'TGCA_C
Cuts at: 3674 4830 3674
Size: 1156 4461
ApoI r'AATT_y
Cuts at: 1493 2467 2926 3152 3304 5413 1493
Size: 974 459 226 152 2109 1697
Fragments arranged by size:
2109 1697 974 459 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4171 160
Size: 872 3139 1606
Fragments arranged by size:
3139 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5617
AvaI C'yCGr_G
Cuts at: 1870 1943 2035 2391 2942 1870
Size: 73 92 356 551 4545
Fragments arranged by size:
4545 551 356 92 73
BamHI G'GATC_C
Cuts at: 2947 3314 2947
Size: 367 5250
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2039 2938 4393 618
Size: 325 217 627 252 899 1455 1842
Fragments arranged by size:
1842 1455 899 627 325 252 217
BanII G_rGCy'C
Cuts at: 1391 2936 1391
Size: 1545 4072
BbeI G_GCGC'C
Cuts at: 2043 2043
Size: 5617
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4646
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3808 3842 1903
Size: 34 1871 34 3678
Fragments arranged by size:
3678 1871 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3509 4719 3509
Size: 1210 4407
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5617
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4228 136
Size: 107 122 71 631 605 2556 1525
Fragments arranged by size:
2556 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2387 2414 5612 2387
Size: 27 3198 2392
Fragments arranged by size:
3198 2392 27
BlpI GC'TnA_GC
Cuts at: 3034 3034
Size: 5617
Bme1580I G_kGCm'C
Cuts at: 1790 3678 4834 1790
Size: 1888 1156 2573
Fragments arranged by size:
2573 1888 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5617
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3348 4306 442
Size: 416 53 83 399 24 1931 958 1753
Fragments arranged by size:
1931 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5617
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2403 2524 4277 1239
Size: 1164 121 1753 2579
Fragments arranged by size:
2579 1753 1164 121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3742 4607 4905 3742
Size: 865 298 4454
Fragments arranged by size:
4454 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4280 882
Size: 617 2781 2219
Fragments arranged by size:
2781 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3408 493
Size: 711 2204 2702
Fragments arranged by size:
2702 2204 711
BsaBI GATnn'nnATC
Cuts at: 2128 2386 5611 2128
Size: 258 3225 2134
Fragments arranged by size:
3225 2134 258
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2040 3424 3806 4441
Size: 53 83 186 936 507 1384 382 635
Cuts at: 4441 275
Size: 1451
Fragments arranged by size:
1451 1384 936 635 507 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2058 2104 4050 4448 4722 4869 5280 2058
Size: 46 1946 398 274 147 411 2395
Fragments arranged by size:
2395 1946 411 398 274 147 46
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2590 2620 1178
Size: 30 165 30 1187 30 4175
Fragments arranged by size:
4175 1187 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5617
BseYI C'CCAG_C
Cuts at: 2574 2611 4820 5316 2574
Size: 37 2209 496 2875
Fragments arranged by size:
2875 2209 496 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2501 1163
Size: 573 765 4279
Fragments arranged by size:
4279 765 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2025 3067 3828 4856 1540
Size: 275 186 24 1042 761 1028 2301
Fragments arranged by size:
2301 1042 1028 761 275 186 24
BsiHKAI G_wGCw'C
Cuts at: 2855 3678 3763 4834 2855
Size: 823 85 1071 3638
Fragments arranged by size:
3638 1071 823 85
BsmI GAATG_Cn'
Cuts at: 3128 3221 3128
Size: 93 5524
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2855 2936 3678 3763 4834 1391
Size: 399 1065 81 742 85 1071 2174
Fragments arranged by size:
2174 1071 1065 742 399 85 81
BspEI T'CCGG_A
Cuts at: 5280 5280
Size: 5617
BspHI T'CATG_A
Cuts at: 3506 5236 3506
Size: 1730 3887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2972 844
Size: 2128 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2508 3504 1394
Size: 429 685 996 3507
Fragments arranged by size:
3507 996 685 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2890 4112 4286 65
Size: 1183 1642 1222 174 1396
Fragments arranged by size:
1642 1396 1222 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2058 4261 4448 1700
Size: 110 248 2203 187 2869
Fragments arranged by size:
2869 2203 248 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5617
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5617
BssSI C'ACGA_G
Cuts at: 3677 4689 3677
Size: 1012 4605
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2471 794
Size: 1677 3940
BstBI TT'CG_AA
Cuts at: 4421 4421
Size: 5617
BstEII G'GTnAC_C
Cuts at: 1184 2321 1184
Size: 1137 4480
BstYI r'GATC_y
Cuts at: 1972 2387 2414 2483 2947 3314 3699 3716 5168
Size: 415 27 69 464 367 385 17 1452
Cuts at: 5168 5254 5266 5612 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 464 415 385 367 346 86
69 27 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2360 2774 513
Size: 552 939 356 414 3356
Fragments arranged by size:
3356 939 552 414 356
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4878
Fragments arranged by size:
4878 683 56
BtsI GCAGTG_nn'
Cuts at: 3133 3927 3947 3133
Size: 794 20 4803
Fragments arranged by size:
4803 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5617
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5582
DraI TTT'AAA
Cuts at: 2921 2921
Size: 5617
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4624 809
Size: 507 3308 1802
Fragments arranged by size:
3308 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 3064 3953
Size: 54 1425 50 275 18 192 1042 889
Cuts at: 3953 5582 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1042 889 275 192 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2022 3064 1537
Size: 275 210 1042 4090
Fragments arranged by size:
4090 1042 275 210
EarI CTCTTCn'nnn_
Cuts at: 1353 2643 2712 2807 3547 1353
Size: 1290 69 95 740 3423
Fragments arranged by size:
3423 1290 740 95 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2045 4204 4578 4724 387
Size: 1064 138 435 21 2159 374 146 1280
Fragments arranged by size:
2159 1280 1064 435 374 146 138 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2403 2524 3680 4277 5064 5332 1239
Size: 393 771 121 1156 597 787 268 1524
Fragments arranged by size:
1524 1156 787 771 597 393 268 121
EcoO109I rG'GnC_Cy
Cuts at: 2603 2603
Size: 5617
EcoRI G'AATT_C
Cuts at: 2926 3304 2926
Size: 378 5239
FspI TGC'GCA
Cuts at: 4123 4123
Size: 5617
HaeII r_GCGC'y
Cuts at: 1678 2043 4764 1678
Size: 365 2721 2531
Fragments arranged by size:
2721 2531 365
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2620 2641 2652 2673 4258
Size: 32 135 32 1048 21 11 21 1585
Cuts at: 4258 4290 4332 4364 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1585 1048 135 42 32 32 32
32 21 21 11
HincII GTy'rAC
Cuts at: 669 1657 1938 2961 3207 669
Size: 988 281 1023 246 3079
Fragments arranged by size:
3079 1023 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5617
HpaI GTT'AAC
Cuts at: 3207 3207
Size: 5617
KasI G'GCGC_C
Cuts at: 2039 2039
Size: 5617
KpnI G_GTAC'C
Cuts at: 2942 2942
Size: 5617
MfeI C'AATT_G
Cuts at: 3216 3216
Size: 5617
MluI A'CGCG_T
Cuts at: 5493 5493
Size: 5617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2168 2357 2547 2666 4731 4915 1305
Size: 863 189 190 119 2065 184 2007
Fragments arranged by size:
2065 2007 863 190 189 184 119
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3795
Fragments arranged by size:
3795 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3575 3934 4093 518
Size: 648 2409 359 159 2042
Fragments arranged by size:
2409 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2045 2615 3033 3710 4858 5103 1606
Size: 439 570 418 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 570 439 418 245
NarI GG'CG_CC
Cuts at: 2040 2040
Size: 5617
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4126
Fragments arranged by size:
4126 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5617
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5617
NotI GC'GGCC_GC
Cuts at: 3064 3064
Size: 5617
NspI r_CATG'y
Cuts at: 1132 1229 2799 2975 4520 1132
Size: 97 1570 176 1545 2229
Fragments arranged by size:
2229 1570 1545 176 97
PasI CC'CwG_GG
Cuts at: 1348 2528 1348
Size: 1180 4437
PciI A'CATG_T
Cuts at: 2795 4516 2795
Size: 1721 3896
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5617
PfoI T'CCnGG_A
Cuts at: 2479 2501 2479
Size: 22 5595
PmeI GTTT'AAAC
Cuts at: 2921 2921
Size: 5617
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3686 3718 5199 5231 3686
Size: 32 1481 32 4072
Fragments arranged by size:
4072 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2796 1613
Size: 1183 4434
PsiI TTA'TAA
Cuts at: 3187 3187
Size: 5617
PstI C_TGCA'G
Cuts at: 830 2969 830
Size: 2139 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5617
PvuII CAG'CTG
Cuts at: 2615 2615
Size: 5617
SalI G'TCGA_C
Cuts at: 1936 2959 1936
Size: 1023 4594
SapI GCTCTTCn'nnn_
Cuts at: 2807 2807
Size: 5617
SbfI CC_TGCA'GG
Cuts at: 2969 2969
Size: 5617
ScaI AGT'ACT
Cuts at: 3865 5577 3865
Size: 1712 3905
SfcI C'TryA_G
Cuts at: 826 1045 2046 2965 4100 4781 4972 826
Size: 219 1001 919 1135 681 191 1471
Fragments arranged by size:
1471 1135 1001 919 681 219 191
SfoI GGC'GCC
Cuts at: 2041 2041
Size: 5617
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5617
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5617
SmaI CCC'GGG
Cuts at: 1872 2944 1872
Size: 1072 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2391 3721 4622 4884 820
Size: 197 926 448 1330 901 262 1553
Fragments arranged by size:
1553 1330 926 901 448 262 197
SnaBI TAC'GTA
Cuts at: 493 3408 493
Size: 2915 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5617
SphI G_CATG'C
Cuts at: 1132 2975 1132
Size: 1843 3774
SspI AAT'ATT
Cuts at: 5 52 3541 5
Size: 47 3489 2081
Fragments arranged by size:
3489 2081 47
StuI AGG'CCT
Cuts at: 1517 2861 1517
Size: 1344 4273
StyI C'CwwG_G
Cuts at: 513 1065 2004 3056 513
Size: 552 939 1052 3074
Fragments arranged by size:
3074 1052 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2919 3644 3661 3814 3999 4444 325
Size: 1255 1339 725 17 153 185 445 1498
Fragments arranged by size:
1498 1339 1255 725 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3863 5575 96
Size: 274 80 33 51 1443 1886 1712 138
Fragments arranged by size:
1886 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2483 2515 2590 2622 2623 2655 5577 5609 2483
Size: 32 75 32 1 32 2922 32 2491
Fragments arranged by size:
2922 2491 75 32 32 32 32 1
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5617
XbaI T'CTAG_A
Cuts at: 2953 2953
Size: 5617
XhoI C'TCGA_G
Cuts at: 1943 2391 1943
Size: 448 5169
XmaI C'CCGG_G
Cuts at: 1870 2942 1870
Size: 1072 4545
XmnI GAAnn'nnTTC
Cuts at: 3746 3746
Size: 5617
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3425 276
Size: 53 83 186 936 1891 2468
Fragments arranged by size:
2468 1891 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI
SwaI XcmI