Restriction map for
pFN21AE3677
(Circular) (Six-base) MAPSORT of: pFN21AE3677.seq Check: 5801 from: 1 to: 5509
pFN21AE3677 5509 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3319 278
Size: 53 83 186 936 1783 2468
Fragments arranged by size:
2468 1783 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2852 1937
Size: 915 4594
Acc65I G'GTAC_C
Cuts at: 2830 2830
Size: 5509
AclI AA'CG_TT
Cuts at: 1563 3636 4009 1563
Size: 2073 373 3063
Fragments arranged by size:
3063 2073 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3572 4956 5224 1632
Size: 1940 1384 268 1917
Fragments arranged by size:
1940 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5312
AflIII A'CryG_T
Cuts at: 1203 4408 5385 1203
Size: 3205 977 1327
Fragments arranged by size:
3205 1327 977
AgeI A'CCGG_T
Cuts at: 4340 4340
Size: 5509
AhdI GACnn_n'nnGTC
Cuts at: 2476 4238 2476
Size: 1762 3747
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5509
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5509
AlwNI CAG_nnn'CTG
Cuts at: 2017 2362 3252 4824 2017
Size: 345 890 1572 2702
Fragments arranged by size:
2702 1572 890 345
ApaI G_GGCC'C
Cuts at: 2066 2066
Size: 5509
ApaLI G'TGCA_C
Cuts at: 3566 4722 3566
Size: 1156 4353
ApoI r'AATT_y
Cuts at: 1493 2818 3044 3196 5305 1493
Size: 1325 226 152 2109 1697
Fragments arranged by size:
2109 1697 1325 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4063 160
Size: 872 3031 1606
Fragments arranged by size:
3031 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5509
AvaI C'yCGr_G
Cuts at: 1870 1943 2484 2634 2645 2834 1870
Size: 73 541 150 11 189 4545
Fragments arranged by size:
4545 541 189 150 73 11
BamHI G'GATC_C
Cuts at: 2609 2839 3206 2609
Size: 230 367 4912
Fragments arranged by size:
4912 367 230
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2180 2344 2454 2830 4285
Size: 325 217 627 393 164 110 376 1455
Cuts at: 4285 618
Size: 1842
Fragments arranged by size:
1842 1455 627 393 376 325 217 164
110
BanII G_rGCy'C
Cuts at: 1391 2066 2168 2635 2805 2828 1391
Size: 675 102 467 170 23 4072
Fragments arranged by size:
4072 675 467 170 102 23
BbeI G_GCGC'C
Cuts at: 2348 2348
Size: 5509
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4538
BbvCI CC'TCA_GC
Cuts at: 2429 2544 2429
Size: 115 5394
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2645 2679 3700 3734 1903
Size: 34 708 34 1021 34 3678
Fragments arranged by size:
3678 1021 708 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2590 3401 4611 2590
Size: 811 1210 3488
Fragments arranged by size:
3488 1210 811
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5509
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2241 2681 4120
Size: 107 122 71 631 605 569 440 1439
Cuts at: 4120 136
Size: 1525
Fragments arranged by size:
1525 1439 631 605 569 440 122 107
71
BglII A'GATC_T
Cuts at: 5504 5504
Size: 5509
BlpI GC'TnA_GC
Cuts at: 2926 2926
Size: 5509
Bme1580I G_kGCm'C
Cuts at: 1790 2024 2066 2504 2690 3570 4726 1790
Size: 234 42 438 186 880 1156 2573
Fragments arranged by size:
2573 1156 880 438 234 186 42
BmgBI CAC'GTC
Cuts at: 1318 2743 1318
Size: 1425 4084
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3240 4198 442
Size: 416 53 83 399 24 1823 958 1753
Fragments arranged by size:
1823 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5509
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2429 2804 4169 1239
Size: 1190 375 1365 2579
Fragments arranged by size:
2579 1365 1190 375
Bpu10I CC'TnA_GC
Cuts at: 2429 2544 2429
Size: 115 5394
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3634 4499 4797 3634
Size: 865 298 4346
Fragments arranged by size:
4346 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2753 4172 882
Size: 617 1254 1419 2219
Fragments arranged by size:
2219 1419 1254 617
BsaAI yAC'GTr
Cuts at: 493 1204 3300 493
Size: 711 2096 2702
Fragments arranged by size:
2702 2096 711
BsaBI GATnn'nnATC
Cuts at: 5503 5503
Size: 5509
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2345 2600 3316 3698
Size: 53 83 186 936 812 255 716 382
Cuts at: 3698 4333 275
Size: 635 1451
Fragments arranged by size:
1451 936 812 716 635 382 255 186
83 53
BsaWI w'CCGG_w
Cuts at: 3942 4340 4614 4761 5172 3942
Size: 398 274 147 411 4279
Fragments arranged by size:
4279 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5284
Fragments arranged by size:
5284 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2450 2813 1186
Size: 1264 363 3882
Fragments arranged by size:
3882 1264 363
BseYI C'CCAG_C
Cuts at: 2010 2143 2569 4712 5208 2010
Size: 133 426 2143 496 2311
Fragments arranged by size:
2311 2143 496 426 133
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2294 2625 1163
Size: 573 558 331 4047
Fragments arranged by size:
4047 573 558 331
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2078 2104 2418 2959 3720 4748
Size: 275 186 77 26 314 541 761 1028
Cuts at: 4748 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 541 314 275 186 77
26
BsiHKAI G_wGCw'C
Cuts at: 3570 3655 4726 3570
Size: 85 1071 4353
Fragments arranged by size:
4353 1071 85
BsmI GAATG_Cn'
Cuts at: 3020 3113 3020
Size: 93 5416
BsmBI CGTCTCn'nnnn_
Cuts at: 2119 2369 2119
Size: 250 5259
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2066 2168 2504 2635 2690 2805
Size: 399 234 42 102 336 131 55 115
Cuts at: 2805 2828 3570 3655 4726 1391
Size: 23 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 336 234 131 115
102 85 55 42 23
BspEI T'CCGG_A
Cuts at: 5172 5172
Size: 5509
BspHI T'CATG_A
Cuts at: 3398 5128 3398
Size: 1730 3779
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2864 844
Size: 2020 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2029 2106 2272 2728 3396 1394
Size: 429 206 77 166 456 668 3507
Fragments arranged by size:
3507 668 456 429 206 166 77
BsrDI GCAATG_nn'
Cuts at: 65 1248 4004 4178 65
Size: 1183 2756 174 1396
Fragments arranged by size:
2756 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2451 2457 4153 4340 1700
Size: 110 641 6 1696 187 2869
Fragments arranged by size:
2869 1696 641 187 110 6
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5509
BssHII G'CGCG_C
Cuts at: 1925 2223 2225 1925
Size: 298 2 5209
Fragments arranged by size:
5209 298 2
BssSI C'ACGA_G
Cuts at: 2312 3569 4581 2312
Size: 1257 1012 3240
Fragments arranged by size:
3240 1257 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2317 794
Size: 1523 3986
BstBI TT'CG_AA
Cuts at: 4313 4313
Size: 5509
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5509
BstXI CCAn_nnnn'nTGG
Cuts at: 2130 2130
Size: 5509
BstYI r'GATC_y
Cuts at: 1972 2609 2839 3206 3591 3608 5060 5146 5158
Size: 637 230 367 385 17 1452 86 12
Cuts at: 5158 5504 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 637 385 367 346 230 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2796 2796
Size: 5509
BtgI C'CryG_G
Cuts at: 513 1065 2004 2157 2340 2349 513
Size: 552 939 153 183 9 3673
Fragments arranged by size:
3673 939 552 183 153 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2717 487
Size: 683 56 1491 3279
Fragments arranged by size:
3279 1491 683 56
BtsI GCAGTG_nn'
Cuts at: 3025 3819 3839 3025
Size: 794 20 4695
Fragments arranged by size:
4695 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5509
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5474
DraI TTT'AAA
Cuts at: 2813 2813
Size: 5509
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4516 809
Size: 507 3200 1802
Fragments arranged by size:
3200 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2075 2101 2174
Size: 54 1425 50 275 18 245 26 73
Cuts at: 2174 2404 2415 2956 3845 5474 8
Size: 230 11 541 889 1629 43
Fragments arranged by size:
1629 1425 889 541 275 245 230 73
54 50 43 26 18 11
EagI C'GGCC_G
Cuts at: 1537 1812 2075 2101 2415 2956 1537
Size: 275 263 26 314 541 4090
Fragments arranged by size:
4090 541 314 275 263 26
EarI CTCTTCn'nnn_
Cuts at: 1353 3439 1353
Size: 2086 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2572 4096 4470 4616 387
Size: 1064 138 983 1524 374 146 1280
Fragments arranged by size:
1524 1280 1064 983 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2429 2804 3572 4169 4956 5224 1239
Size: 393 797 375 768 597 787 268 1524
Fragments arranged by size:
1524 797 787 768 597 393 375 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2432 2432
Size: 5509
EcoO109I rG'GnC_Cy
Cuts at: 2062 2191 2478 2062
Size: 129 287 5093
Fragments arranged by size:
5093 287 129
EcoRI G'AATT_C
Cuts at: 2818 3196 2818
Size: 378 5131
FspI TGC'GCA
Cuts at: 2285 2291 4015 2285
Size: 6 1724 3779
Fragments arranged by size:
3779 1724 6
FspAI rTGC'GCAy
Cuts at: 2291 2291
Size: 5509
HaeII r_GCGC'y
Cuts at: 1678 2265 2348 2452 4656 1678
Size: 587 83 104 2204 2531
Fragments arranged by size:
2531 2204 587 104 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2462 2494 4150 4182 4224
Size: 32 135 32 890 32 1656 32 42
Cuts at: 4224 4256 1373
Size: 32 2626
Fragments arranged by size:
2626 1656 890 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2853 3099 669
Size: 988 281 915 246 3079
Fragments arranged by size:
3079 988 915 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5509
HpaI GTT'AAC
Cuts at: 3099 3099
Size: 5509
KasI G'GCGC_C
Cuts at: 2344 2344
Size: 5509
KpnI G_GTAC'C
Cuts at: 2834 2834
Size: 5509
MfeI C'AATT_G
Cuts at: 3108 3108
Size: 5509
MluI A'CGCG_T
Cuts at: 5385 5385
Size: 5509
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2112 2736 4623 4807 1305
Size: 807 624 1887 184 2007
Fragments arranged by size:
2007 1887 807 624 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3687
Fragments arranged by size:
3687 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3467 3826 3985 518
Size: 648 2301 359 159 2042
Fragments arranged by size:
2301 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2247 2351 2497 2925 3602 4750 4995 1606
Size: 641 104 146 428 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 641 428 245 146 104
NaeI GCC'GGC
Cuts at: 2459 2459
Size: 5509
NarI GG'CG_CC
Cuts at: 2345 2345
Size: 5509
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4018
Fragments arranged by size:
4018 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5509
NgoMIV G'CCGG_C
Cuts at: 2457 2457
Size: 5509
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5509
NotI GC'GGCC_GC
Cuts at: 2075 2101 2956 2075
Size: 26 855 4628
Fragments arranged by size:
4628 855 26
NspI r_CATG'y
Cuts at: 1132 1229 2290 2867 4412 1132
Size: 97 1061 577 1545 2229
Fragments arranged by size:
2229 1545 1061 577 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5509
PciI A'CATG_T
Cuts at: 4408 4408
Size: 5509
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5509
PfoI T'CCnGG_A
Cuts at: 2039 2187 2039
Size: 148 5361
PmeI GTTT'AAAC
Cuts at: 2813 2813
Size: 5509
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3578 3610 5091 5123 3578
Size: 32 1481 32 3964
Fragments arranged by size:
3964 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2191 2478 2191
Size: 287 5222
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5509
PsiI TTA'TAA
Cuts at: 3079 3079
Size: 5509
PspOMI G'GGCC_C
Cuts at: 2062 2062
Size: 5509
PstI C_TGCA'G
Cuts at: 830 2861 830
Size: 2031 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5509
SacII CC_GC'GG
Cuts at: 2352 2352
Size: 5509
SalI G'TCGA_C
Cuts at: 1936 2851 1936
Size: 915 4594
SanDI GG'GwC_CC
Cuts at: 2191 2478 2191
Size: 287 5222
SbfI CC_TGCA'GG
Cuts at: 2861 2861
Size: 5509
ScaI AGT'ACT
Cuts at: 3757 5469 3757
Size: 1712 3797
SfcI C'TryA_G
Cuts at: 826 1045 2857 3992 4673 4864 826
Size: 219 1812 1135 681 191 1471
Fragments arranged by size:
1812 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2346 2346
Size: 5509
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5509
SgrAI Cr'CCGG_yG
Cuts at: 1700 2451 1700
Size: 751 4758
SmaI CCC'GGG
Cuts at: 1872 2836 1872
Size: 964 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3613 4514 4776 820
Size: 197 926 1670 901 262 1553
Fragments arranged by size:
1670 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3300 493
Size: 2807 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5509
SphI G_CATG'C
Cuts at: 1132 2290 2867 1132
Size: 1158 577 3774
Fragments arranged by size:
3774 1158 577
SspI AAT'ATT
Cuts at: 5 52 3433 5
Size: 47 3381 2081
Fragments arranged by size:
3381 2081 47
StuI AGG'CCT
Cuts at: 1517 2427 1517
Size: 910 4599
StyI C'CwwG_G
Cuts at: 513 1065 2004 2805 2948 513
Size: 552 939 801 143 3074
Fragments arranged by size:
3074 939 801 552 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3536 3553 3706 3891 4336 325
Size: 1255 1956 17 153 185 445 1498
Fragments arranged by size:
1956 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3755 5467 96
Size: 274 80 33 51 1443 1778 1712 138
Fragments arranged by size:
1778 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5469 5501 5469
Size: 32 5477
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5509
XbaI T'CTAG_A
Cuts at: 2845 2845
Size: 5509
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5509
XmaI C'CCGG_G
Cuts at: 1870 2834 1870
Size: 964 4545
XmnI GAAnn'nnTTC
Cuts at: 3638 3638
Size: 5509
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3317 276
Size: 53 83 186 936 1783 2468
Fragments arranged by size:
2468 1783 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacII SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI NruI
NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI
SapI SexAI SfiI SrfI SwaI XcmI