Restriction map for
pFN21AE3675
(Circular) (Six-base) MAPSORT of: pFN21AE3675.seq Check: 5732 from: 1 to: 5803
pFN21AE3675 5803 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3047 3613 278
Size: 53 83 186 936 1511 566 2468
Fragments arranged by size:
2468 1511 936 566 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3146 1937
Size: 1209 4594
Acc65I G'GTAC_C
Cuts at: 3124 3124
Size: 5803
AclI AA'CG_TT
Cuts at: 1563 3930 4303 1563
Size: 2367 373 3063
Fragments arranged by size:
3063 2367 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2138 2396 2632 3866 5250 5518 1632
Size: 506 258 236 1234 1384 268 1917
Fragments arranged by size:
1917 1384 1234 506 268 258 236
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5606
AflIII A'CryG_T
Cuts at: 1203 4702 5679 1203
Size: 3499 977 1327
Fragments arranged by size:
3499 1327 977
AgeI A'CCGG_T
Cuts at: 4634 4634
Size: 5803
AhdI GACnn_n'nnGTC
Cuts at: 4532 4532
Size: 5803
AleI CACnn'nnGTG
Cuts at: 1166 2954 1166
Size: 1788 4015
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2379 1184
Size: 1195 4608
AlwNI CAG_nnn'CTG
Cuts at: 3071 3546 5118 3071
Size: 475 1572 3756
Fragments arranged by size:
3756 1572 475
ApaLI G'TGCA_C
Cuts at: 3860 5016 3860
Size: 1156 4647
ApoI r'AATT_y
Cuts at: 1493 2372 3029 3061 3112 3338 3490 5599 1493
Size: 879 657 32 51 226 152 2109 1697
Fragments arranged by size:
2109 1697 879 657 226 152 51 32
AseI AT'TA_AT
Cuts at: 160 1032 4357 160
Size: 872 3325 1606
Fragments arranged by size:
3325 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5803
AvaI C'yCGr_G
Cuts at: 1870 1943 3128 1870
Size: 73 1185 4545
Fragments arranged by size:
4545 1185 73
BamHI G'GATC_C
Cuts at: 3133 3500 3133
Size: 367 5436
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2042 3124 4579 618
Size: 325 217 627 255 1082 1455 1842
Fragments arranged by size:
1842 1455 1082 627 325 255 217
BanII G_rGCy'C
Cuts at: 1391 3122 1391
Size: 1731 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2821 928
Size: 971 922 3910
Fragments arranged by size:
3910 971 922
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3994 4028 1903
Size: 34 2057 34 3678
Fragments arranged by size:
3678 2057 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3695 4905 3695
Size: 1210 4593
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5803
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4414 136
Size: 107 122 71 631 605 2742 1525
Fragments arranged by size:
2742 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5798 5798
Size: 5803
BlpI GC'TnA_GC
Cuts at: 3220 3220
Size: 5803
Bme1580I G_kGCm'C
Cuts at: 1790 2045 3864 5020 1790
Size: 255 1819 1156 2573
Fragments arranged by size:
2573 1819 1156 255
BmgBI CAC'GTC
Cuts at: 1318 2238 1318
Size: 920 4883
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3534 4492 442
Size: 416 53 83 399 24 2117 958 1753
Fragments arranged by size:
2117 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5803
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2845 4463 1239
Size: 1606 1618 2579
Fragments arranged by size:
2579 1618 1606
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3000 3928 4793 5091 3000
Size: 928 865 298 3712
Fragments arranged by size:
3712 928 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4466 882
Size: 617 2967 2219
Fragments arranged by size:
2967 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3594 493
Size: 711 2390 2702
Fragments arranged by size:
2702 2390 711
BsaBI GATnn'nnATC
Cuts at: 5797 5797
Size: 5803
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2289 3044 3610 3992
Size: 53 83 186 936 756 755 566 382
Cuts at: 3992 4627 275
Size: 635 1451
Fragments arranged by size:
1451 936 756 755 635 566 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4236 4634 4908 5055 5466 4236
Size: 398 274 147 411 4573
Fragments arranged by size:
4573 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2009 2039 2129 2159 2177
Size: 30 165 30 606 30 90 30 18
Cuts at: 2177 2207 1178
Size: 30 4774
Fragments arranged by size:
4774 606 165 90 30 30 30 30
30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2432 1186
Size: 1246 4557
BseYI C'CCAG_C
Cuts at: 2208 5006 5502 2208
Size: 2798 496 2509
Fragments arranged by size:
2798 2509 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2747 2780 1163
Size: 573 1011 33 4186
Fragments arranged by size:
4186 1011 573 33
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3253 4014 5042 1540
Size: 275 186 1252 761 1028 2301
Fragments arranged by size:
2301 1252 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3864 3949 5020 3864
Size: 85 1071 4647
Fragments arranged by size:
4647 1071 85
BsmI GAATG_Cn'
Cuts at: 3314 3407 3314
Size: 93 5710
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 3122 3864 3949 5020 1391
Size: 399 255 1077 742 85 1071 2174
Fragments arranged by size:
2174 1077 1071 742 399 255 85
BspEI T'CCGG_A
Cuts at: 5466 5466
Size: 5803
BspHI T'CATG_A
Cuts at: 2368 3692 5422 2368
Size: 1324 1730 2749
Fragments arranged by size:
2749 1730 1324
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2482 3158 844
Size: 1638 676 3489
Fragments arranged by size:
3489 1638 676
BsrBI CCG'CTC
Cuts at: 1394 1823 3690 1394
Size: 429 1867 3507
Fragments arranged by size:
3507 1867 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2262 4298 4472 65
Size: 1183 1014 2036 174 1396
Fragments arranged by size:
2036 1396 1183 1014 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4447 4634 1700
Size: 110 2637 187 2869
Fragments arranged by size:
2869 2637 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5803
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5803
BssSI C'ACGA_G
Cuts at: 3863 4875 3863
Size: 1012 4791
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5803
BstBI TT'CG_AA
Cuts at: 4607 4607
Size: 5803
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5803
BstXI CCAn_nnnn'nTGG
Cuts at: 2755 2755
Size: 5803
BstYI r'GATC_y
Cuts at: 1972 3133 3500 3885 3902 5354 5440 5452 5798
Size: 1161 367 385 17 1452 86 12 346
Cuts at: 5798 1972
Size: 1977
Fragments arranged by size:
1977 1452 1161 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5251
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2005 487
Size: 683 56 779 4285
Fragments arranged by size:
4285 779 683 56
BtsI GCAGTG_nn'
Cuts at: 2877 2929 3319 4113 4133 2877
Size: 52 390 794 20 4547
Fragments arranged by size:
4547 794 390 52 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5803
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5768
DraI TTT'AAA
Cuts at: 2379 3107 2379
Size: 728 5075
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4810 809
Size: 507 3494 1802
Fragments arranged by size:
3494 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3250 4139 5768
Size: 54 1425 50 275 18 1420 889 1629
Cuts at: 5768 8
Size: 43
Fragments arranged by size:
1629 1425 1420 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3250 1537
Size: 275 1438 4090
Fragments arranged by size:
4090 1438 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2855 3733 1353
Size: 1502 878 3423
Fragments arranged by size:
3423 1502 878
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2261 4390 4764 4910 387
Size: 1064 138 672 2129 374 146 1280
Fragments arranged by size:
2129 1280 1064 672 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2138 2396 2632 2845 3866 4463 5250
Size: 393 506 258 236 213 1021 597 787
Cuts at: 5250 5518 1239
Size: 268 1524
Fragments arranged by size:
1524 1021 787 597 506 393 268 258
236 213
EcoNI CCTnn'n_nnAGG
Cuts at: 2414 2414
Size: 5803
EcoRI G'AATT_C
Cuts at: 3029 3112 3490 3029
Size: 83 378 5342
Fragments arranged by size:
5342 378 83
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2245 2606 2245
Size: 361 5442
FspI TGC'GCA
Cuts at: 4309 4309
Size: 5803
HaeII r_GCGC'y
Cuts at: 1678 4950 1678
Size: 3272 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4444 4476 4518 4550 1373
Size: 32 135 32 2872 32 42 32 2626
Fragments arranged by size:
2872 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3147 3393 669
Size: 988 281 1209 246 3079
Fragments arranged by size:
3079 1209 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5803
HpaI GTT'AAC
Cuts at: 3393 3393
Size: 5803
KpnI G_GTAC'C
Cuts at: 3128 3128
Size: 5803
MfeI C'AATT_G
Cuts at: 3402 3402
Size: 5803
MluI A'CGCG_T
Cuts at: 5679 5679
Size: 5803
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4917 5101 1305
Size: 3612 184 2007
Fragments arranged by size:
3612 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3981
Fragments arranged by size:
3981 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2954 3761 4120 4279 518
Size: 648 1788 807 359 159 2042
Fragments arranged by size:
2042 1788 807 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2794 3219 3896 5044 5289 1606
Size: 1188 425 677 1148 245 2120
Fragments arranged by size:
2120 1188 1148 677 425 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5251
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5803
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5803
NotI GC'GGCC_GC
Cuts at: 3250 3250
Size: 5803
NspI r_CATG'y
Cuts at: 1132 1229 2136 2356 2700 2890 3161 4706 1132
Size: 97 907 220 344 190 271 1545 2229
Fragments arranged by size:
2229 1545 907 344 271 220 190 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5803
PciI A'CATG_T
Cuts at: 4702 4702
Size: 5803
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5803
PfoI T'CCnGG_A
Cuts at: 2181 2181
Size: 5803
PmeI GTTT'AAAC
Cuts at: 3107 3107
Size: 5803
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2832 2864 3872 3904 5385 5417 2832
Size: 32 1008 32 1481 32 3218
Fragments arranged by size:
3218 1481 1008 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2073 1613
Size: 460 5343
PsiI TTA'TAA
Cuts at: 3373 3373
Size: 5803
PstI C_TGCA'G
Cuts at: 830 2705 3155 830
Size: 1875 450 3478
Fragments arranged by size:
3478 1875 450
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5803
PvuII CAG'CTG
Cuts at: 2794 2794
Size: 5803
SalI G'TCGA_C
Cuts at: 1936 3145 1936
Size: 1209 4594
SapI GCTCTTCn'nnn_
Cuts at: 2855 2855
Size: 5803
SbfI CC_TGCA'GG
Cuts at: 3155 3155
Size: 5803
ScaI AGT'ACT
Cuts at: 4051 5763 4051
Size: 1712 4091
SfcI C'TryA_G
Cuts at: 826 1045 2701 3151 4286 4967 5158 826
Size: 219 1656 450 1135 681 191 1471
Fragments arranged by size:
1656 1471 1135 681 450 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5803
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5803
SmaI CCC'GGG
Cuts at: 1872 3130 1872
Size: 1258 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3015 3907 4808 5070 820
Size: 197 926 1072 892 901 262 1553
Fragments arranged by size:
1553 1072 926 901 892 262 197
SnaBI TAC'GTA
Cuts at: 493 3594 493
Size: 3101 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5803
SphI G_CATG'C
Cuts at: 1132 3161 1132
Size: 2029 3774
SspI AAT'ATT
Cuts at: 5 52 2684 3727 5
Size: 47 2632 1043 2081
Fragments arranged by size:
2632 2081 1043 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5803
StyI C'CwwG_G
Cuts at: 513 1065 3242 513
Size: 552 2177 3074
Fragments arranged by size:
3074 2177 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3830 3847 4000 4185 4630 325
Size: 1255 2250 17 153 185 445 1498
Fragments arranged by size:
2250 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2163 2781 4049
Size: 274 80 33 51 1443 186 618 1268
Cuts at: 4049 5761 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1268 618 274 186 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5763 5795 5763
Size: 32 5771
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5803
XbaI T'CTAG_A
Cuts at: 3139 3139
Size: 5803
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2035 2499 2035
Size: 464 5339
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5803
XmaI C'CCGG_G
Cuts at: 1870 3128 1870
Size: 1258 4545
XmnI GAAnn'nnTTC
Cuts at: 2586 3932 2586
Size: 1346 4457
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3045 3611 276
Size: 53 83 186 936 1511 566 2468
Fragments arranged by size:
2468 1511 936 566 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI