Restriction map for
pFN21AE3674
(Circular) (Six-base) MAPSORT of: pFN21AE3674.seq Check: 6413 from: 1 to: 5902
pFN21AE3674 5902 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3712 278
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2748 3245 1937
Size: 811 497 4594
Fragments arranged by size:
4594 811 497
Acc65I G'GTAC_C
Cuts at: 2631 3223 2631
Size: 592 5310
AclI AA'CG_TT
Cuts at: 1563 4029 4402 1563
Size: 2466 373 3063
Fragments arranged by size:
3063 2466 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2120 2969 3965 5349 5617 1632
Size: 488 849 996 1384 268 1917
Fragments arranged by size:
1917 1384 996 849 488 268
AfeI AGC'GCT
Cuts at: 2309 2309
Size: 5902
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5705
AflIII A'CryG_T
Cuts at: 1203 4801 5778 1203
Size: 3598 977 1327
Fragments arranged by size:
3598 1327 977
AgeI A'CCGG_T
Cuts at: 2183 4733 2183
Size: 2550 3352
AhdI GACnn_n'nnGTC
Cuts at: 4631 4631
Size: 5902
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5902
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2416 1184
Size: 1232 4670
AlwNI CAG_nnn'CTG
Cuts at: 3645 5217 3645
Size: 1572 4330
ApaLI G'TGCA_C
Cuts at: 3959 5115 3959
Size: 1156 4746
ApoI r'AATT_y
Cuts at: 1493 2673 2768 3211 3437 3589 5698 1493
Size: 1180 95 443 226 152 2109 1697
Fragments arranged by size:
2109 1697 1180 443 226 152 95
AseI AT'TA_AT
Cuts at: 160 1032 4456 160
Size: 872 3424 1606
Fragments arranged by size:
3424 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
AvaI C'yCGr_G
Cuts at: 1870 1943 2172 3227 1870
Size: 73 229 1055 4545
Fragments arranged by size:
4545 1055 229 73
BamHI G'GATC_C
Cuts at: 3232 3599 3232
Size: 367 5535
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2180 2631 2829 2837 3223
Size: 325 217 627 393 451 198 8 386
Cuts at: 3223 4678 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 451 393 386 325 217
198 8
BanII G_rGCy'C
Cuts at: 1391 3221 1391
Size: 1830 4072
BbeI G_GCGC'C
Cuts at: 2841 2841
Size: 5902
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4931
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4093 4127 1903
Size: 34 2156 34 3678
Fragments arranged by size:
3678 2156 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3794 5004 3794
Size: 1210 4692
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5902
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4513 136
Size: 107 122 71 631 605 2841 1525
Fragments arranged by size:
2841 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2812 5897 2812
Size: 3085 2817
BlpI GC'TnA_GC
Cuts at: 3319 3319
Size: 5902
Bme1580I G_kGCm'C
Cuts at: 1790 3963 5119 1790
Size: 2173 1156 2573
Fragments arranged by size:
2573 2173 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5902
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2736 3633 4591
Size: 416 53 83 399 24 1319 897 958
Cuts at: 4591 442
Size: 1753
Fragments arranged by size:
1753 1319 958 897 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5902
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2106 2106
Size: 5902
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2592 4562 1239
Size: 1353 1970 2579
Fragments arranged by size:
2579 1970 1353
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4027 4892 5190 4027
Size: 865 298 4739
Fragments arranged by size:
4739 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4565 882
Size: 617 3066 2219
Fragments arranged by size:
3066 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3693 493
Size: 711 2489 2702
Fragments arranged by size:
2702 2489 711
BsaBI GATnn'nnATC
Cuts at: 2910 5896 2910
Size: 2986 2916
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2838 3709 4091 4726
Size: 53 83 186 936 1305 871 382 635
Cuts at: 4726 275
Size: 1451
Fragments arranged by size:
1451 1305 936 871 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2183 4335 4733 5007 5154 5565 2183
Size: 2152 398 274 147 411 2520
Fragments arranged by size:
2520 2152 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5677
Fragments arranged by size:
5677 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5902
BseYI C'CCAG_C
Cuts at: 2196 2696 5105 5601 2196
Size: 500 2409 496 2497
Fragments arranged by size:
2497 2409 500 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5329
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3352 4113 5141 1540
Size: 275 186 1351 761 1028 2301
Fragments arranged by size:
2301 1351 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3963 4048 5119 3963
Size: 85 1071 4746
Fragments arranged by size:
4746 1071 85
BsmI GAATG_Cn'
Cuts at: 2992 3413 3506 2992
Size: 421 93 5388
Fragments arranged by size:
5388 421 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3221 3963 4048 5119 1391
Size: 399 1431 742 85 1071 2174
Fragments arranged by size:
2174 1431 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5565 5565
Size: 5902
BspHI T'CATG_A
Cuts at: 3791 5521 3791
Size: 1730 4172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2192 3168 3257 844
Size: 1348 976 89 3489
Fragments arranged by size:
3489 1348 976 89
BsrBI CCG'CTC
Cuts at: 1394 1823 3789 1394
Size: 429 1966 3507
Fragments arranged by size:
3507 1966 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4397 4571 65
Size: 1183 3149 174 1396
Fragments arranged by size:
3149 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2183 4546 4733 1700
Size: 110 373 2363 187 2869
Fragments arranged by size:
2869 2363 373 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5902
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5902
BssSI C'ACGA_G
Cuts at: 3962 4974 3962
Size: 1012 4890
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2207 2987 794
Size: 1413 780 3709
Fragments arranged by size:
3709 1413 780
BstBI TT'CG_AA
Cuts at: 4706 4706
Size: 5902
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5902
BstYI r'GATC_y
Cuts at: 1972 2812 3232 3599 3984 4001 5453 5539 5551
Size: 840 420 367 385 17 1452 86 12
Cuts at: 5551 5897 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 840 420 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2833 2971 513
Size: 552 939 829 138 3444
Fragments arranged by size:
3444 939 829 552 138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5163
Fragments arranged by size:
5163 683 56
BtsI GCAGTG_nn'
Cuts at: 2355 3418 4212 4232 2355
Size: 1063 794 20 4025
Fragments arranged by size:
4025 1063 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5902
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5867
DraI TTT'AAA
Cuts at: 3206 3206
Size: 5902
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3164 4909 809
Size: 507 1848 1745 1802
Fragments arranged by size:
1848 1802 1745 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2352 3349
Size: 54 1425 50 275 18 225 297 997
Cuts at: 3349 4238 5867 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 997 889 297 275 225 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3349 1537
Size: 275 1537 4090
Fragments arranged by size:
4090 1537 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2493 2585 3832 1353
Size: 1140 92 1247 3423
Fragments arranged by size:
3423 1247 1140 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4489 4863 5009 387
Size: 1064 138 2900 374 146 1280
Fragments arranged by size:
2900 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2120 2592 2969 3965 4562 5349 5617
Size: 393 488 472 377 996 597 787 268
Cuts at: 5617 1239
Size: 1524
Fragments arranged by size:
1524 996 787 597 488 472 393 377
268
EcoNI CCTnn'n_nnAGG
Cuts at: 3020 3020
Size: 5902
EcoO109I rG'GnC_Cy
Cuts at: 2245 2339 3045 2245
Size: 94 706 5102
Fragments arranged by size:
5102 706 94
EcoRI G'AATT_C
Cuts at: 3211 3589 3211
Size: 378 5524
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2614 2614
Size: 5902
FspI TGC'GCA
Cuts at: 4408 4408
Size: 5902
HaeII r_GCGC'y
Cuts at: 1678 2311 2841 5049 1678
Size: 633 530 2208 2531
Fragments arranged by size:
2531 2208 633 530
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2422 2454 4543 4575 4617
Size: 32 135 32 850 32 2089 32 42
Cuts at: 4617 4649 1373
Size: 32 2626
Fragments arranged by size:
2626 2089 850 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3246 3492 669
Size: 988 281 1308 246 3079
Fragments arranged by size:
3079 1308 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5902
HpaI GTT'AAC
Cuts at: 3492 3492
Size: 5902
KasI G'GCGC_C
Cuts at: 2837 2837
Size: 5902
KpnI G_GTAC'C
Cuts at: 2635 3227 2635
Size: 592 5310
MfeI C'AATT_G
Cuts at: 3501 3501
Size: 5902
MluI A'CGCG_T
Cuts at: 5778 5778
Size: 5902
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5016 5200 1305
Size: 3711 184 2007
Fragments arranged by size:
3711 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4080
Fragments arranged by size:
4080 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2229 2274 3860 4219 4378 518
Size: 648 1063 45 1586 359 159 2042
Fragments arranged by size:
2042 1586 1063 648 359 159 45
MspA1I CmG'CkG
Cuts at: 1606 2357 2764 3318 3995 5143 5388 1606
Size: 751 407 554 677 1148 245 2120
Fragments arranged by size:
2120 1148 751 677 554 407 245
NarI GG'CG_CC
Cuts at: 2838 2838
Size: 5902
NcoI C'CATG_G
Cuts at: 513 1065 2004 2833 513
Size: 552 939 829 3582
Fragments arranged by size:
3582 939 829 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5902
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5902
NotI GC'GGCC_GC
Cuts at: 3349 3349
Size: 5902
NspI r_CATG'y
Cuts at: 1132 1229 2860 3260 4805 1132
Size: 97 1631 400 1545 2229
Fragments arranged by size:
2229 1631 1545 400 97
PasI CC'CwG_GG
Cuts at: 1348 2843 1348
Size: 1495 4407
PciI A'CATG_T
Cuts at: 4801 4801
Size: 5902
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5902
PmeI GTTT'AAAC
Cuts at: 3206 3206
Size: 5902
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3971 4003 5484 5516 3971
Size: 32 1481 32 4357
Fragments arranged by size:
4357 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2245 2245
Size: 5902
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5902
PsiI TTA'TAA
Cuts at: 2043 3472 2043
Size: 1429 4473
PstI C_TGCA'G
Cuts at: 830 2983 3254 830
Size: 2153 271 3478
Fragments arranged by size:
3478 2153 271
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5902
PvuII CAG'CTG
Cuts at: 2764 2764
Size: 5902
SalI G'TCGA_C
Cuts at: 1936 3244 1936
Size: 1308 4594
SanDI GG'GwC_CC
Cuts at: 2245 2245
Size: 5902
SapI GCTCTTCn'nnn_
Cuts at: 2493 2493
Size: 5902
SbfI CC_TGCA'GG
Cuts at: 3254 3254
Size: 5902
ScaI AGT'ACT
Cuts at: 4150 5862 4150
Size: 1712 4190
SfcI C'TryA_G
Cuts at: 826 1045 2258 2979 3250 4385 5066 5257 826
Size: 219 1213 721 271 1135 681 191 1471
Fragments arranged by size:
1471 1213 1135 721 681 271 219 191
SfoI GGC'GCC
Cuts at: 2839 2839
Size: 5902
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5902
SmaI CCC'GGG
Cuts at: 1872 3229 1872
Size: 1357 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4006 4907 5169 820
Size: 197 926 2063 901 262 1553
Fragments arranged by size:
2063 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3693 493
Size: 3200 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5902
SphI G_CATG'C
Cuts at: 1132 3260 1132
Size: 2128 3774
SspI AAT'ATT
Cuts at: 5 52 2642 3826 5
Size: 47 2590 1184 2081
Fragments arranged by size:
2590 2081 1184 47
StuI AGG'CCT
Cuts at: 1517 2154 2870 1517
Size: 637 716 4549
Fragments arranged by size:
4549 716 637
StyI C'CwwG_G
Cuts at: 513 1065 2004 2713 2833 3341 513
Size: 552 939 709 120 508 3074
Fragments arranged by size:
3074 939 709 552 508 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3929 3946 4099 4284 4729 325
Size: 1255 2349 17 153 185 445 1498
Fragments arranged by size:
2349 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4148 5860 96
Size: 274 80 33 51 1443 2171 1712 138
Fragments arranged by size:
2171 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5862 5894 5862
Size: 32 5870
Tth111I GACn'n_nGTC
Cuts at: 1316 2753 1316
Size: 1437 4465
XbaI T'CTAG_A
Cuts at: 3238 3238
Size: 5902
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2831 2840 2831
Size: 9 5893
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5902
XmaI C'CCGG_G
Cuts at: 1870 3227 1870
Size: 1357 4545
XmnI GAAnn'nnTTC
Cuts at: 2523 4031 2523
Size: 1508 4394
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3710 276
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SexAI SfiI SrfI SwaI