Restriction map for
pFN21AE3673
(Circular) (Six-base) MAPSORT of: pFN21AE3673.seq Check: 6041 from: 1 to: 5815
pFN21AE3673 5815 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3625 278
Size: 53 83 186 936 2089 2468
Fragments arranged by size:
2468 2089 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3158 1937
Size: 1221 4594
Acc65I G'GTAC_C
Cuts at: 3136 3136
Size: 5815
AclI AA'CG_TT
Cuts at: 1563 3942 4315 1563
Size: 2379 373 3063
Fragments arranged by size:
3063 2379 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2801 2856 3878 5262 5530 1632
Size: 1169 55 1022 1384 268 1917
Fragments arranged by size:
1917 1384 1169 1022 268 55
AflII C'TTAA_G
Cuts at: 820 1017 2650 820
Size: 197 1633 3985
Fragments arranged by size:
3985 1633 197
AflIII A'CryG_T
Cuts at: 1203 4714 5691 1203
Size: 3511 977 1327
Fragments arranged by size:
3511 1327 977
AgeI A'CCGG_T
Cuts at: 4646 4646
Size: 5815
AhdI GACnn_n'nnGTC
Cuts at: 4544 4544
Size: 5815
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5815
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2391 1184
Size: 1207 4608
AlwNI CAG_nnn'CTG
Cuts at: 3558 5130 3558
Size: 1572 4243
ApaLI G'TGCA_C
Cuts at: 3872 5028 3872
Size: 1156 4659
ApoI r'AATT_y
Cuts at: 1493 2030 2384 3041 3124 3350 3502 5611 1493
Size: 537 354 657 83 226 152 2109 1697
Fragments arranged by size:
2109 1697 657 537 354 226 152 83
AseI AT'TA_AT
Cuts at: 160 1032 4369 160
Size: 872 3337 1606
Fragments arranged by size:
3337 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5815
AvaI C'yCGr_G
Cuts at: 1870 1943 3108 3140 1870
Size: 73 1165 32 4545
Fragments arranged by size:
4545 1165 73 32
AvrII C'CTAG_G
Cuts at: 2225 2225
Size: 5815
BamHI G'GATC_C
Cuts at: 3145 3512 3145
Size: 367 5448
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2054 3136 4591 618
Size: 325 217 627 267 1082 1455 1842
Fragments arranged by size:
1842 1455 1082 627 325 267 217
BanII G_rGCy'C
Cuts at: 1391 3134 1391
Size: 1743 4072
BbeI G_GCGC'C
Cuts at: 2058 2058
Size: 5815
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4844
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2894 2928 4006 4040 1903
Size: 34 957 34 1078 34 3678
Fragments arranged by size:
3678 1078 957 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3707 4917 3707
Size: 1210 4605
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5815
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4426 136
Size: 107 122 71 631 605 2754 1525
Fragments arranged by size:
2754 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2260 5810 2260
Size: 3550 2265
BlpI GC'TnA_GC
Cuts at: 2254 3232 2254
Size: 978 4837
Bme1580I G_kGCm'C
Cuts at: 1790 3876 5032 1790
Size: 2086 1156 2573
Fragments arranged by size:
2573 2086 1156
BmgBI CAC'GTC
Cuts at: 1318 2620 1318
Size: 1302 4513
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3546 4504 442
Size: 416 53 83 399 24 2129 958 1753
Fragments arranged by size:
2129 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5815
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4475 1239
Size: 3236 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3940 4805 5103 3940
Size: 865 298 4652
Fragments arranged by size:
4652 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4478 882
Size: 617 2979 2219
Fragments arranged by size:
2979 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3606 493
Size: 711 2402 2702
Fragments arranged by size:
2702 2402 711
BsaBI GATnn'nnATC
Cuts at: 5809 5809
Size: 5815
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2055 3622 4004 4639
Size: 53 83 186 936 522 1567 382 635
Cuts at: 4639 275
Size: 1451
Fragments arranged by size:
1567 1451 936 635 522 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2101 4248 4646 4920 5067 5478 2101
Size: 2147 398 274 147 411 2438
Fragments arranged by size:
2438 2147 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5590
Fragments arranged by size:
5590 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5815
BseYI C'CCAG_C
Cuts at: 5018 5514 5018
Size: 496 5319
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2701 1163
Size: 573 965 4277
Fragments arranged by size:
4277 965 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3265 4026 5054 1540
Size: 275 186 1264 761 1028 2301
Fragments arranged by size:
2301 1264 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2115 3876 3961 5032 2115
Size: 1761 85 1071 2898
Fragments arranged by size:
2898 1761 1071 85
BsmI GAATG_Cn'
Cuts at: 3326 3419 3326
Size: 93 5722
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2115 3134 3876 3961 5032 1391
Size: 399 325 1019 742 85 1071 2174
Fragments arranged by size:
2174 1071 1019 742 399 325 85
BspEI T'CCGG_A
Cuts at: 5478 5478
Size: 5815
BspHI T'CATG_A
Cuts at: 2977 3704 5434 2977
Size: 727 1730 3358
Fragments arranged by size:
3358 1730 727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2153 3170 844
Size: 1309 1017 3489
Fragments arranged by size:
3489 1309 1017
BsrBI CCG'CTC
Cuts at: 1394 1823 3702 1394
Size: 429 1879 3507
Fragments arranged by size:
3507 1879 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4310 4484 65
Size: 1183 3062 174 1396
Fragments arranged by size:
3062 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4459 4646 1700
Size: 110 2649 187 2869
Fragments arranged by size:
2869 2649 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5815
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5815
BssSI C'ACGA_G
Cuts at: 3875 4887 3875
Size: 1012 4803
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5815
BstBI TT'CG_AA
Cuts at: 4619 4619
Size: 5815
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5815
BstYI r'GATC_y
Cuts at: 1972 2260 3145 3512 3897 3914 5366 5452 5464
Size: 288 885 367 385 17 1452 86 12
Cuts at: 5464 5810 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 885 385 367 346 288 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2049 513
Size: 552 939 45 4279
Fragments arranged by size:
4279 939 552 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5076
Fragments arranged by size:
5076 683 56
BtsI GCAGTG_nn'
Cuts at: 2644 2689 3331 4125 4145 2644
Size: 45 642 794 20 4314
Fragments arranged by size:
4314 794 642 45 20
ClaI AT'CG_AT
Cuts at: 1554 2812 1554
Size: 1258 4557
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5780
DraI TTT'AAA
Cuts at: 2791 3119 2791
Size: 328 5487
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4822 809
Size: 507 3506 1802
Fragments arranged by size:
3506 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3262 4151 5780
Size: 54 1425 50 275 18 1432 889 1629
Cuts at: 5780 8
Size: 43
Fragments arranged by size:
1629 1432 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3262 1537
Size: 275 1450 4090
Fragments arranged by size:
4090 1450 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2270 2424 2700 2777 3745 1353
Size: 917 154 276 77 968 3423
Fragments arranged by size:
3423 968 917 276 154 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4402 4776 4922 387
Size: 1064 138 2813 374 146 1280
Fragments arranged by size:
2813 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2801 2856 3878 4475 5262 5530 1239
Size: 393 1169 55 1022 597 787 268 1524
Fragments arranged by size:
1524 1169 1022 787 597 393 268 55
EcoRI G'AATT_C
Cuts at: 2384 3041 3124 3502 2384
Size: 657 83 378 4697
Fragments arranged by size:
4697 657 378 83
EcoRV GAT'ATC
Cuts at: 3064 3064
Size: 5815
FspI TGC'GCA
Cuts at: 4321 4321
Size: 5815
HaeII r_GCGC'y
Cuts at: 1678 2058 4962 1678
Size: 380 2904 2531
Fragments arranged by size:
2904 2531 380
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2755 2787 4456 4488 4530
Size: 32 135 32 1183 32 1669 32 42
Cuts at: 4530 4562 1373
Size: 32 2626
Fragments arranged by size:
2626 1669 1183 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2222 2617 3054 3159 3405 669
Size: 988 281 284 395 437 105 246 3079
Fragments arranged by size:
3079 988 437 395 284 281 246 105
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5815
HpaI GTT'AAC
Cuts at: 3405 3405
Size: 5815
KasI G'GCGC_C
Cuts at: 2054 2054
Size: 5815
KpnI G_GTAC'C
Cuts at: 3140 3140
Size: 5815
MfeI C'AATT_G
Cuts at: 2409 3414 2409
Size: 1005 4810
MluI A'CGCG_T
Cuts at: 5691 5691
Size: 5815
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2278 4929 5113 1305
Size: 973 2651 184 2007
Fragments arranged by size:
2651 2007 973 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3993
Fragments arranged by size:
3993 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3773 4132 4291 518
Size: 648 2607 359 159 2042
Fragments arranged by size:
2607 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3231 3908 5056 5301 1606
Size: 1625 677 1148 245 2120
Fragments arranged by size:
2120 1625 1148 677 245
NarI GG'CG_CC
Cuts at: 2055 2055
Size: 5815
NcoI C'CATG_G
Cuts at: 513 1065 2004 2049 513
Size: 552 939 45 4279
Fragments arranged by size:
4279 939 552 45
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5815
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5815
NotI GC'GGCC_GC
Cuts at: 3262 3262
Size: 5815
NspI r_CATG'y
Cuts at: 1132 1229 2253 2368 3173 4718 1132
Size: 97 1024 115 805 1545 2229
Fragments arranged by size:
2229 1545 1024 805 115 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5815
PciI A'CATG_T
Cuts at: 4714 4714
Size: 5815
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5815
PmeI GTTT'AAAC
Cuts at: 3119 3119
Size: 5815
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3884 3916 5397 5429 3884
Size: 32 1481 32 4270
Fragments arranged by size:
4270 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5815
PsiI TTA'TAA
Cuts at: 3385 3385
Size: 5815
PstI C_TGCA'G
Cuts at: 830 3167 830
Size: 2337 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5815
SalI G'TCGA_C
Cuts at: 1936 3157 1936
Size: 1221 4594
SbfI CC_TGCA'GG
Cuts at: 3167 3167
Size: 5815
ScaI AGT'ACT
Cuts at: 4063 5775 4063
Size: 1712 4103
SfcI C'TryA_G
Cuts at: 826 1045 3163 4298 4979 5170 826
Size: 219 2118 1135 681 191 1471
Fragments arranged by size:
2118 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2056 2056
Size: 5815
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5815
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5815
SmaI CCC'GGG
Cuts at: 1872 3142 1872
Size: 1270 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2650 3919 4820 5082 820
Size: 197 926 707 1269 901 262 1553
Fragments arranged by size:
1553 1269 926 901 707 262 197
SnaBI TAC'GTA
Cuts at: 493 3606 493
Size: 3113 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5815
SphI G_CATG'C
Cuts at: 1132 3173 1132
Size: 2041 3774
SspI AAT'ATT
Cuts at: 5 52 2476 3739 5
Size: 47 2424 1263 2081
Fragments arranged by size:
2424 2081 1263 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5815
StyI C'CwwG_G
Cuts at: 513 1065 2004 2049 2225 3254 513
Size: 552 939 45 176 1029 3074
Fragments arranged by size:
3074 1029 939 552 176 45
SwaI ATTT'AAAT
Cuts at: 2791 2791
Size: 5815
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3842 3859 4012 4197 4642 325
Size: 1255 2262 17 153 185 445 1498
Fragments arranged by size:
2262 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4061 5773 96
Size: 274 80 33 51 1443 2084 1712 138
Fragments arranged by size:
2084 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5775 5807 5775
Size: 32 5783
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5815
XbaI T'CTAG_A
Cuts at: 3151 3151
Size: 5815
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2047 2511 2047
Size: 464 5351
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5815
XmaI C'CCGG_G
Cuts at: 1870 3140 1870
Size: 1270 4545
XmnI GAAnn'nnTTC
Cuts at: 2097 2202 3944 2097
Size: 105 1742 3968
Fragments arranged by size:
3968 1742 105
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3623 276
Size: 53 83 186 936 2089 2468
Fragments arranged by size:
2468 2089 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SrfI