Restriction map for
pFN21AE3672
(Circular) (Six-base) MAPSORT of: pFN21AE3672.seq Check: 9827 from: 1 to: 6352
pFN21AE3672 6352 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4162 278
Size: 53 83 186 936 2626 2468
Fragments arranged by size:
2626 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3695 1937
Size: 1758 4594
Acc65I G'GTAC_C
Cuts at: 3673 3673
Size: 6352
AclI AA'CG_TT
Cuts at: 1563 2694 4479 4852 1563
Size: 1131 1785 373 3063
Fragments arranged by size:
3063 1785 1131 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2164 2552 2864 3468 4415 5799 6067 1632
Size: 532 388 312 604 947 1384 268 1917
Fragments arranged by size:
1917 1384 947 604 532 388 312 268
AflII C'TTAA_G
Cuts at: 820 1017 3287 820
Size: 197 2270 3885
Fragments arranged by size:
3885 2270 197
AflIII A'CryG_T
Cuts at: 1203 5251 6228 1203
Size: 4048 977 1327
Fragments arranged by size:
4048 1327 977
AgeI A'CCGG_T
Cuts at: 5183 5183
Size: 6352
AhdI GACnn_n'nnGTC
Cuts at: 5081 5081
Size: 6352
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6352
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6352
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2297 2329 2297
Size: 32 6320
AlwNI CAG_nnn'CTG
Cuts at: 2039 4095 5667 2039
Size: 2056 1572 2724
Fragments arranged by size:
2724 2056 1572
ApaLI G'TGCA_C
Cuts at: 4409 5565 4409
Size: 1156 5196
ApoI r'AATT_y
Cuts at: 1493 2525 2596 2630 2940 3298 3318 3661 3887
Size: 1032 71 34 310 358 20 343 226
Cuts at: 3887 4039 6148 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1032 358 343 310 226 152
71 34 20
AseI AT'TA_AT
Cuts at: 160 1032 2667 4906 160
Size: 872 1635 2239 1606
Fragments arranged by size:
2239 1635 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6352
AvaI C'yCGr_G
Cuts at: 1870 1943 3677 1870
Size: 73 1734 4545
Fragments arranged by size:
4545 1734 73
BamHI G'GATC_C
Cuts at: 2495 3488 3682 4049 2495
Size: 993 194 367 4798
Fragments arranged by size:
4798 993 367 194
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3673 5128 618
Size: 325 217 627 1886 1455 1842
Fragments arranged by size:
1886 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3671 1391
Size: 2280 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2001 928
Size: 971 102 5279
Fragments arranged by size:
5279 971 102
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3346 3380 4543 4577 1903
Size: 34 1409 34 1163 34 3678
Fragments arranged by size:
3678 1409 1163 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2231 4244 5454 2231
Size: 2013 1210 3129
Fragments arranged by size:
3129 2013 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6352
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3377 4963 136
Size: 107 122 71 631 605 1705 1586 1525
Fragments arranged by size:
1705 1586 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6347 6347
Size: 6352
BlpI GC'TnA_GC
Cuts at: 3006 3769 3006
Size: 763 5589
Bme1580I G_kGCm'C
Cuts at: 1790 4413 5569 1790
Size: 2623 1156 2573
Fragments arranged by size:
2623 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6352
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3024 4083 5041
Size: 416 53 83 399 24 1607 1059 958
Cuts at: 5041 442
Size: 1753
Fragments arranged by size:
1753 1607 1059 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2168 1056
Size: 1112 5240
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2505 3096 5012 1239
Size: 1266 591 1916 2579
Fragments arranged by size:
2579 1916 1266 591
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4477 5342 5640 4477
Size: 865 298 5189
Fragments arranged by size:
5189 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2975 5015 882
Size: 617 1476 2040 2219
Fragments arranged by size:
2219 2040 1476 617
BsaAI yAC'GTr
Cuts at: 493 1204 4143 493
Size: 711 2939 2702
Fragments arranged by size:
2939 2702 711
BsaBI GATnn'nnATC
Cuts at: 6346 6346
Size: 6352
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4159 4541 5176 275
Size: 53 83 186 936 2626 382 635 1451
Fragments arranged by size:
2626 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4785 5183 5457 5604 6015 4785
Size: 398 274 147 411 5122
Fragments arranged by size:
5122 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2368 2398 3383 3413 1178
Size: 30 165 30 965 30 985 30 4117
Fragments arranged by size:
4117 985 965 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2990 2993 1186
Size: 1804 3 4545
Fragments arranged by size:
4545 1804 3
BseYI C'CCAG_C
Cuts at: 2227 2321 2344 5555 6051 2227
Size: 94 23 3211 496 2528
Fragments arranged by size:
3211 2528 496 94 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5779
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3802 4563 5591 1540
Size: 275 186 1801 761 1028 2301
Fragments arranged by size:
2301 1801 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3591 4413 4498 5569 3591
Size: 822 85 1071 4374
Fragments arranged by size:
4374 1071 822 85
BsmI GAATG_Cn'
Cuts at: 2393 2721 3117 3863 3956 2393
Size: 328 396 746 93 4789
Fragments arranged by size:
4789 746 396 328 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3591 3671 4413 4498 5569 1391
Size: 399 1801 80 742 85 1071 2174
Fragments arranged by size:
2174 1801 1071 742 399 85 80
BspEI T'CCGG_A
Cuts at: 6015 6015
Size: 6352
BspHI T'CATG_A
Cuts at: 3294 4241 5971 3294
Size: 947 1730 3675
Fragments arranged by size:
3675 1730 947
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3707 844
Size: 2863 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4239 1394
Size: 429 2416 3507
Fragments arranged by size:
3507 2416 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4847 5021 65
Size: 1183 3599 174 1396
Fragments arranged by size:
3599 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4996 5183 1700
Size: 110 3186 187 2869
Fragments arranged by size:
3186 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2120 96
Size: 2024 4328
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6352
BssSI C'ACGA_G
Cuts at: 4412 5424 4412
Size: 1012 5340
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2204 2824 794
Size: 1410 620 4322
Fragments arranged by size:
4322 1410 620
BstBI TT'CG_AA
Cuts at: 5156 5156
Size: 6352
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6352
BstYI r'GATC_y
Cuts at: 1972 2495 3488 3682 4049 4434 4451 5903 5989
Size: 523 993 194 367 385 17 1452 86
Cuts at: 5989 6001 6347 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 993 523 385 367 346 194
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3045 513
Size: 552 1980 3820
Fragments arranged by size:
3820 1980 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5613
Fragments arranged by size:
5613 683 56
BtsI GCAGTG_nn'
Cuts at: 2461 3868 4662 4682 2461
Size: 1407 794 20 4131
Fragments arranged by size:
4131 1407 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6352
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6317
DraI TTT'AAA
Cuts at: 2601 3656 2601
Size: 1055 5297
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5359 809
Size: 507 4043 1802
Fragments arranged by size:
4043 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3799 4688 6317
Size: 54 1425 50 275 18 1969 889 1629
Cuts at: 6317 8
Size: 43
Fragments arranged by size:
1969 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3799 1537
Size: 275 1987 4090
Fragments arranged by size:
4090 1987 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2752 4282 1353
Size: 1399 1530 3423
Fragments arranged by size:
3423 1530 1399
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4939 5313 5459 387
Size: 1064 138 3350 374 146 1280
Fragments arranged by size:
3350 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2164 2505 2552 2864 3096 3468 4415
Size: 393 532 341 47 312 232 372 947
Cuts at: 4415 5012 5799 6067 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 947 787 597 532 393 372 341
312 268 232 47
EcoRI G'AATT_C
Cuts at: 2940 3298 3661 4039 2940
Size: 358 363 378 5253
Fragments arranged by size:
5253 378 363 358
FspI TGC'GCA
Cuts at: 4858 4858
Size: 6352
HaeII r_GCGC'y
Cuts at: 1678 5499 1678
Size: 3821 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2368 2400 3383 3415 4993
Size: 32 135 32 796 32 983 32 1578
Cuts at: 4993 5025 5067 5099 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1578 983 796 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3696 3942 669
Size: 988 281 1758 246 3079
Fragments arranged by size:
3079 1758 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2905 3265 3324 748
Size: 2157 360 59 3776
Fragments arranged by size:
3776 2157 360 59
HpaI GTT'AAC
Cuts at: 3942 3942
Size: 6352
KpnI G_GTAC'C
Cuts at: 3677 3677
Size: 6352
MfeI C'AATT_G
Cuts at: 3951 3951
Size: 6352
MluI A'CGCG_T
Cuts at: 6228 6228
Size: 6352
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2811 5466 5650 1305
Size: 1506 2655 184 2007
Fragments arranged by size:
2655 2007 1506 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4530
Fragments arranged by size:
4530 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2150 2714 4310 4669 4828 518
Size: 648 984 564 1596 359 159 2042
Fragments arranged by size:
2042 1596 984 648 564 359 159
MspA1I CmG'CkG
Cuts at: 1606 2227 3768 4445 5593 5838 1606
Size: 621 1541 677 1148 245 2120
Fragments arranged by size:
2120 1541 1148 677 621 245
NcoI C'CATG_G
Cuts at: 513 1065 3045 513
Size: 552 1980 3820
Fragments arranged by size:
3820 1980 552
NdeI CA'TA_TG
Cuts at: 387 2728 387
Size: 2341 4011
NheI G'CTAG_C
Cuts at: 1052 2164 1052
Size: 1112 5240
NotI GC'GGCC_GC
Cuts at: 3799 3799
Size: 6352
NspI r_CATG'y
Cuts at: 1132 1229 2827 3653 3710 5255 1132
Size: 97 1598 826 57 1545 2229
Fragments arranged by size:
2229 1598 1545 826 97 57
PasI CC'CwG_GG
Cuts at: 1348 3484 1348
Size: 2136 4216
PciI A'CATG_T
Cuts at: 5251 5251
Size: 6352
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6352
PfoI T'CCnGG_A
Cuts at: 2949 3215 2949
Size: 266 6086
PmeI GTTT'AAAC
Cuts at: 3656 3656
Size: 6352
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4421 4453 5934 5966 4421
Size: 32 1481 32 4807
Fragments arranged by size:
4807 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6352
PsiI TTA'TAA
Cuts at: 3922 3922
Size: 6352
PstI C_TGCA'G
Cuts at: 830 2456 3704 830
Size: 1626 1248 3478
Fragments arranged by size:
3478 1626 1248
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6352
PvuII CAG'CTG
Cuts at: 2227 2227
Size: 6352
SalI G'TCGA_C
Cuts at: 1936 3694 1936
Size: 1758 4594
SapI GCTCTTCn'nnn_
Cuts at: 2752 2752
Size: 6352
SbfI CC_TGCA'GG
Cuts at: 3704 3704
Size: 6352
ScaI AGT'ACT
Cuts at: 4600 6312 4600
Size: 1712 4640
SexAI A'CCwGG_T
Cuts at: 3507 3507
Size: 6352
SfcI C'TryA_G
Cuts at: 826 1045 2452 3700 4835 5516 5707 826
Size: 219 1407 1248 1135 681 191 1471
Fragments arranged by size:
1471 1407 1248 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6352
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6352
SmaI CCC'GGG
Cuts at: 1872 3679 1872
Size: 1807 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3287 4456 5357 5619 820
Size: 197 926 1344 1169 901 262 1553
Fragments arranged by size:
1553 1344 1169 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4143 493
Size: 3650 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6352
SphI G_CATG'C
Cuts at: 1132 3710 1132
Size: 2578 3774
SspI AAT'ATT
Cuts at: 5 52 4276 5
Size: 47 4224 2081
Fragments arranged by size:
4224 2081 47
StuI AGG'CCT
Cuts at: 1517 3607 1517
Size: 2090 4262
StyI C'CwwG_G
Cuts at: 513 1065 2612 3045 3546 3791 513
Size: 552 1547 433 501 245 3074
Fragments arranged by size:
3074 1547 552 501 433 245
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4379 4396 4549 4734 5179 325
Size: 1255 2799 17 153 185 445 1498
Fragments arranged by size:
2799 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2120 4598 6310
Size: 274 80 33 51 1443 143 2478 1712
Cuts at: 6310 96
Size: 138
Fragments arranged by size:
2478 1712 1443 274 143 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2229 2261 6312 6344 2229
Size: 32 4051 32 2237
Fragments arranged by size:
4051 2237 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3509 1316
Size: 2193 4159
XbaI T'CTAG_A
Cuts at: 3688 3688
Size: 6352
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6352
XmaI C'CCGG_G
Cuts at: 1870 3677 1870
Size: 1807 4545
XmnI GAAnn'nnTTC
Cuts at: 4481 4481
Size: 6352
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4160 276
Size: 53 83 186 936 2626 2468
Fragments arranged by size:
2626 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SfiI SfoI SrfI SwaI XcmI