Restriction map for
pFN21AE3671
(Circular) (Six-base) MAPSORT of: pFN21AE3671.seq Check: 2015 from: 1 to: 7615
pFN21AE3671 7615 bp
With 182 enzymes: SgfI *
March 28, 2012 12:20 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5425 278
Size: 53 83 186 936 3889 2468
Fragments arranged by size:
3889 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2082 2398 4958 1937
Size: 145 316 2560 4594
Fragments arranged by size:
4594 2560 316 145
Acc65I G'GTAC_C
Cuts at: 4936 4936
Size: 7615
AclI AA'CG_TT
Cuts at: 1563 5742 6115 1563
Size: 4179 373 3063
Fragments arranged by size:
4179 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2760 2997 3899 5678 7062 7330 1632
Size: 1128 237 902 1779 1384 268 1917
Fragments arranged by size:
1917 1779 1384 1128 902 268 237
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7418
AflIII A'CryG_T
Cuts at: 1203 6514 7491 1203
Size: 5311 977 1327
Fragments arranged by size:
5311 1327 977
AgeI A'CCGG_T
Cuts at: 6446 6446
Size: 7615
AhdI GACnn_n'nnGTC
Cuts at: 6344 6344
Size: 7615
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7615
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3477 4545 1184
Size: 2293 1068 4254
Fragments arranged by size:
4254 2293 1068
AlwNI CAG_nnn'CTG
Cuts at: 4607 4867 5358 6930 4607
Size: 260 491 1572 5292
Fragments arranged by size:
5292 1572 491 260
ApaLI G'TGCA_C
Cuts at: 2562 5672 6828 2562
Size: 3110 1156 3349
Fragments arranged by size:
3349 3110 1156
ApoI r'AATT_y
Cuts at: 1493 2806 4153 4924 5150 5302 7411 1493
Size: 1313 1347 771 226 152 2109 1697
Fragments arranged by size:
2109 1697 1347 1313 771 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6169 160
Size: 872 5137 1606
Fragments arranged by size:
5137 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7615
AvaI C'yCGr_G
Cuts at: 1870 1943 4940 1870
Size: 73 2997 4545
Fragments arranged by size:
4545 2997 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3109 3142 3558 3591 3109
Size: 33 416 33 7133
Fragments arranged by size:
7133 416 33 33
BamHI G'GATC_C
Cuts at: 4945 5312 4945
Size: 367 7248
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4546 4936 6391 618
Size: 325 217 627 2759 390 1455 1842
Fragments arranged by size:
2759 1842 1455 627 390 325 217
BanII G_rGCy'C
Cuts at: 1391 4934 1391
Size: 3543 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2766 2850 2941 4637 4767 4863 928
Size: 971 867 84 91 1696 130 96 3680
Fragments arranged by size:
3680 1696 971 867 130 96 91 84
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5806 5840 1903
Size: 34 3869 34 3678
Fragments arranged by size:
3869 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5507 6717 5507
Size: 1210 6405
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7615
BfrBI ATG'CAT
Cuts at: 3887 4140 4522 3887
Size: 253 382 6980
Fragments arranged by size:
6980 382 253
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6226 136
Size: 107 122 71 631 605 4554 1525
Fragments arranged by size:
4554 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3146 4094 7610 3146
Size: 948 3516 3151
Fragments arranged by size:
3516 3151 948
BlpI GC'TnA_GC
Cuts at: 5032 5032
Size: 7615
Bme1580I G_kGCm'C
Cuts at: 1790 2566 5676 6832 1790
Size: 776 3110 1156 2573
Fragments arranged by size:
3110 2573 1156 776
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7615
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4744 5346 6304
Size: 416 53 83 399 24 3327 602 958
Cuts at: 6304 442
Size: 1753
Fragments arranged by size:
3327 1753 958 602 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7615
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3280 3280
Size: 7615
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 6275 1239
Size: 5036 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3156 3876 5740 6605 6903 3156
Size: 720 1864 865 298 3868
Fragments arranged by size:
3868 1864 865 720 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2620 6278 882
Size: 617 1121 3658 2219
Fragments arranged by size:
3658 2219 1121 617
BsaAI yAC'GTr
Cuts at: 493 1204 5406 493
Size: 711 4202 2702
Fragments arranged by size:
4202 2702 711
BsaBI GATnn'nnATC
Cuts at: 3351 4099 7609 3351
Size: 748 3510 3357
Fragments arranged by size:
3510 3357 748
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5422 5804 6439 275
Size: 53 83 186 936 3889 382 635 1451
Fragments arranged by size:
3889 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6048 6446 6720 6867 7278 6048
Size: 398 274 147 411 6385
Fragments arranged by size:
6385 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7390
Fragments arranged by size:
7390 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3255 3869 4307 4449 1186
Size: 2069 614 438 142 4352
Fragments arranged by size:
4352 2069 614 438 142
BseYI C'CCAG_C
Cuts at: 6818 7314 6818
Size: 496 7119
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3630 4781 1163
Size: 573 1894 1151 3997
Fragments arranged by size:
3997 1894 1151 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5065 5826 6854 1540
Size: 275 186 3064 761 1028 2301
Fragments arranged by size:
3064 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2566 4393 5676 5761 6832 2566
Size: 1827 1283 85 1071 3349
Fragments arranged by size:
3349 1827 1283 1071 85
BsmI GAATG_Cn'
Cuts at: 5126 5219 5126
Size: 93 7522
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2566 4393 4934 5676 5761 6832 1391
Size: 399 776 1827 541 742 85 1071 2174
Fragments arranged by size:
2174 1827 1071 776 742 541 399 85
BspEI T'CCGG_A
Cuts at: 7278 7278
Size: 7615
BspHI T'CATG_A
Cuts at: 5504 7234 5504
Size: 1730 5885
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2021 4970 844
Size: 1177 2949 3489
Fragments arranged by size:
3489 2949 1177
BsrBI CCG'CTC
Cuts at: 1394 1823 5502 1394
Size: 429 3679 3507
Fragments arranged by size:
3679 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2194 6110 6284 65
Size: 1183 946 3916 174 1396
Fragments arranged by size:
3916 1396 1183 946 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6259 6446 1700
Size: 110 4449 187 2869
Fragments arranged by size:
4449 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2148 4081 96
Size: 2052 1933 3630
Fragments arranged by size:
3630 2052 1933
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7615
BssSI C'ACGA_G
Cuts at: 5675 6687 5675
Size: 1012 6603
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7615
BstBI TT'CG_AA
Cuts at: 6419 6419
Size: 7615
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7615
BstYI r'GATC_y
Cuts at: 1972 3146 3329 3697 4094 4945 5312 5697 5714
Size: 1174 183 368 397 851 367 385 17
Cuts at: 5714 7166 7252 7264 7610 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1174 851 397 385 368 367
346 183 86 17 12
BstZ17I GTA'TAC
Cuts at: 2399 2399
Size: 7615
Bsu36I CC'TnA_GG
Cuts at: 4443 4443
Size: 7615
BtgI C'CryG_G
Cuts at: 513 1065 2004 3740 513
Size: 552 939 1736 4388
Fragments arranged by size:
4388 1736 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6876
Fragments arranged by size:
6876 683 56
BtsI GCAGTG_nn'
Cuts at: 5131 5925 5945 5131
Size: 794 20 6801
Fragments arranged by size:
6801 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7615
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7580
DraI TTT'AAA
Cuts at: 2041 3304 4260 4919 2041
Size: 1263 956 659 4737
Fragments arranged by size:
4737 1263 956 659
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4246 6622 809
Size: 507 2930 2376 1802
Fragments arranged by size:
2930 2376 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5062 5951 7580
Size: 54 1425 50 275 18 3232 889 1629
Cuts at: 7580 8
Size: 43
Fragments arranged by size:
3232 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5062 1537
Size: 275 3250 4090
Fragments arranged by size:
4090 3250 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3073 3249 4362 4627 5545 1353
Size: 1720 176 1113 265 918 3423
Fragments arranged by size:
3423 1720 1113 918 265 176
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6202 6576 6722 387
Size: 1064 138 4613 374 146 1280
Fragments arranged by size:
4613 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2760 2997 3899 5678 6275 7062 7330
Size: 393 1128 237 902 1779 597 787 268
Cuts at: 7330 1239
Size: 1524
Fragments arranged by size:
1779 1524 1128 902 787 597 393 268
237
EcoO109I rG'GnC_Cy
Cuts at: 2410 2722 4746 2410
Size: 312 2024 5279
Fragments arranged by size:
5279 2024 312
EcoRI G'AATT_C
Cuts at: 4153 4924 5302 4153
Size: 771 378 6466
Fragments arranged by size:
6466 771 378
EcoRV GAT'ATC
Cuts at: 3921 3921
Size: 7615
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2790 3929 4661 2790
Size: 1139 732 5744
Fragments arranged by size:
5744 1139 732
FspI TGC'GCA
Cuts at: 6121 6121
Size: 7615
HaeII r_GCGC'y
Cuts at: 1678 6762 1678
Size: 5084 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2365 2397 2573 2605 3872
Size: 32 135 32 793 32 176 32 1267
Cuts at: 3872 3904 4484 4516 6256 6288 6330 6362 1373
Size: 32 580 32 1740 32 42 32 2626
Fragments arranged by size:
2626 1740 1267 793 580 176 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4710 4959 5205 669
Size: 988 281 2772 249 246 3079
Fragments arranged by size:
3079 2772 988 281 249 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7615
HpaI GTT'AAC
Cuts at: 4710 5205 4710
Size: 495 7120
KpnI G_GTAC'C
Cuts at: 4940 4940
Size: 7615
MfeI C'AATT_G
Cuts at: 2201 2850 5214 2201
Size: 649 2364 4602
Fragments arranged by size:
4602 2364 649
MluI A'CGCG_T
Cuts at: 7491 7491
Size: 7615
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2026 3697 4562 4922 6729 6913 1305
Size: 721 1671 865 360 1807 184 2007
Fragments arranged by size:
2007 1807 1671 865 721 360 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5793
Fragments arranged by size:
5793 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2372 2391 4145 4226 4320 5573 5932
Size: 648 1206 19 1754 81 94 1253 359
Cuts at: 5932 6091 518
Size: 159 2042
Fragments arranged by size:
2042 1754 1253 1206 648 359 159 94
81 19
MspA1I CmG'CkG
Cuts at: 1606 5031 5708 6856 7101 1606
Size: 3425 677 1148 245 2120
Fragments arranged by size:
3425 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3740 513
Size: 552 939 1736 4388
Fragments arranged by size:
4388 1736 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7615
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7615
NotI GC'GGCC_GC
Cuts at: 5062 5062
Size: 7615
NsiI A_TGCA'T
Cuts at: 3889 4142 4524 3889
Size: 253 382 6980
Fragments arranged by size:
6980 382 253
NspI r_CATG'y
Cuts at: 1132 1229 2867 4973 6518 1132
Size: 97 1638 2106 1545 2229
Fragments arranged by size:
2229 2106 1638 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2593 1348
Size: 1245 6370
PciI A'CATG_T
Cuts at: 6514 6514
Size: 7615
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3962 1108
Size: 2854 4761
PfoI T'CCnGG_A
Cuts at: 3379 3379
Size: 7615
PmeI GTTT'AAAC
Cuts at: 4919 4919
Size: 7615
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5684 5716 7197 7229 5684
Size: 32 1481 32 6070
Fragments arranged by size:
6070 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2410 2722 4746 2410
Size: 312 2024 5279
Fragments arranged by size:
5279 2024 312
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7615
PsiI TTA'TAA
Cuts at: 2232 5185 2232
Size: 2953 4662
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4420 4452 4420
Size: 32 7583
PstI C_TGCA'G
Cuts at: 830 4967 830
Size: 4137 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7615
SalI G'TCGA_C
Cuts at: 1936 4957 1936
Size: 3021 4594
SanDI GG'GwC_CC
Cuts at: 4746 4746
Size: 7615
SbfI CC_TGCA'GG
Cuts at: 4967 4967
Size: 7615
ScaI AGT'ACT
Cuts at: 4432 5863 7575 4432
Size: 1431 1712 4472
Fragments arranged by size:
4472 1712 1431
SexAI A'CCwGG_T
Cuts at: 4423 4423
Size: 7615
SfcI C'TryA_G
Cuts at: 826 1045 2083 4599 4963 6098 6779 6970 826
Size: 219 1038 2516 364 1135 681 191 1471
Fragments arranged by size:
2516 1471 1135 1038 681 364 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7615
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7615
SmaI CCC'GGG
Cuts at: 1872 4942 1872
Size: 3070 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3171 3891 5719 6620 6882 820
Size: 197 926 1228 720 1828 901 262 1553
Fragments arranged by size:
1828 1553 1228 926 901 720 262 197
SnaBI TAC'GTA
Cuts at: 493 5406 493
Size: 4913 2702
SpeI A'CTAG_T
Cuts at: 152 4433 4712 152
Size: 4281 279 3055
Fragments arranged by size:
4281 3055 279
SphI G_CATG'C
Cuts at: 1132 2867 4973 1132
Size: 1735 2106 3774
Fragments arranged by size:
3774 2106 1735
SspI AAT'ATT
Cuts at: 5 52 5539 5
Size: 47 5487 2081
Fragments arranged by size:
5487 2081 47
StuI AGG'CCT
Cuts at: 1517 4448 1517
Size: 2931 4684
StyI C'CwwG_G
Cuts at: 513 1065 2004 2718 3740 5054 513
Size: 552 939 714 1022 1314 3074
Fragments arranged by size:
3074 1314 1022 939 714 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3283 5642 5659 5812 5997 6442 325
Size: 1255 1703 2359 17 153 185 445 1498
Fragments arranged by size:
2359 1703 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2148 4081 4430
Size: 274 80 33 51 1443 171 1933 349
Cuts at: 4430 5861 7573 96
Size: 1431 1712 138
Fragments arranged by size:
1933 1712 1443 1431 349 274 171 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7575 7607 7575
Size: 32 7583
Tth111I GACn'n_nGTC
Cuts at: 1316 2648 4587 1316
Size: 1332 1939 4344
Fragments arranged by size:
4344 1939 1332
XbaI T'CTAG_A
Cuts at: 4951 4951
Size: 7615
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3829 4047 3829
Size: 218 7397
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7615
XmaI C'CCGG_G
Cuts at: 1870 4940 1870
Size: 3070 4545
XmnI GAAnn'nnTTC
Cuts at: 2324 2809 3912 5744 2324
Size: 485 1103 1832 4195
Fragments arranged by size:
4195 1832 1103 485
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5423 276
Size: 53 83 186 936 3889 2468
Fragments arranged by size:
3889 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI
SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
Bpu10I BsiWI BsmBI BstXI DraIII EcoICRI EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspOMI PspXI PvuII RsrII SacI SacII SapI SfiI
SfoI SrfI SwaI