Restriction map for
pFN21AE3669
(Circular) (Six-base) MAPSORT of: pFN21AE3669.seq Check: 2452 from: 1 to: 6094
pFN21AE3669 6094 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3904 278
Size: 53 83 186 936 2368 2468
Fragments arranged by size:
2468 2368 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3437 1937
Size: 1500 4594
Acc65I G'GTAC_C
Cuts at: 3415 3415
Size: 6094
AclI AA'CG_TT
Cuts at: 1563 4221 4594 1563
Size: 2658 373 3063
Fragments arranged by size:
3063 2658 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2213 3149 3206 4157 5541 5809 1632
Size: 581 936 57 951 1384 268 1917
Fragments arranged by size:
1917 1384 951 936 581 268 57
AfeI AGC'GCT
Cuts at: 2501 2501
Size: 6094
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5897
AflIII A'CryG_T
Cuts at: 1203 2760 2946 2976 4993 5970 1203
Size: 1557 186 30 2017 977 1327
Fragments arranged by size:
2017 1557 1327 977 186 30
AgeI A'CCGG_T
Cuts at: 4925 4925
Size: 6094
AhdI GACnn_n'nnGTC
Cuts at: 2992 4823 2992
Size: 1831 4263
AleI CACnn'nnGTG
Cuts at: 1166 2867 1166
Size: 1701 4393
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3030 1184
Size: 1846 4248
AlwNI CAG_nnn'CTG
Cuts at: 3837 5409 3837
Size: 1572 4522
ApaLI G'TGCA_C
Cuts at: 4151 5307 4151
Size: 1156 4938
ApoI r'AATT_y
Cuts at: 1493 3403 3629 3781 5890 1493
Size: 1910 226 152 2109 1697
Fragments arranged by size:
2109 1910 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4648 160
Size: 872 3616 1606
Fragments arranged by size:
3616 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6094
AvaI C'yCGr_G
Cuts at: 1870 1943 3052 3419 1870
Size: 73 1109 367 4545
Fragments arranged by size:
4545 1109 367 73
BamHI G'GATC_C
Cuts at: 2159 3424 3791 2159
Size: 1265 367 4462
Fragments arranged by size:
4462 1265 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2788 3217 3344 3415 4870
Size: 325 217 627 1001 429 127 71 1455
Cuts at: 4870 618
Size: 1842
Fragments arranged by size:
1842 1455 1001 627 429 325 217 127
71
BanII G_rGCy'C
Cuts at: 1391 2616 3413 1391
Size: 1225 797 4072
Fragments arranged by size:
4072 1225 797
BbeI G_GCGC'C
Cuts at: 3348 3348
Size: 6094
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2024 928
Size: 971 125 4998
Fragments arranged by size:
4998 971 125
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2203 2237 4285 4319 1903
Size: 34 266 34 2048 34 3678
Fragments arranged by size:
3678 2048 266 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3986 5196 3986
Size: 1210 4884
BclI T'GATC_A
Cuts at: 1548 2310 1548
Size: 762 5332
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2420 4705 136
Size: 107 122 71 631 605 748 2285 1525
Fragments arranged by size:
2285 1525 748 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6089 6089
Size: 6094
BlpI GC'TnA_GC
Cuts at: 3511 3511
Size: 6094
Bme1580I G_kGCm'C
Cuts at: 1790 2571 2580 2793 4155 5311 1790
Size: 781 9 213 1362 1156 2573
Fragments arranged by size:
2573 1362 1156 781 213 9
BmgBI CAC'GTC
Cuts at: 1318 2761 1318
Size: 1443 4651
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3247 3825 4783
Size: 416 53 83 399 24 1830 578 958
Cuts at: 4783 442
Size: 1753
Fragments arranged by size:
1830 1753 958 578 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6094
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2925 3265 2925
Size: 340 5754
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2630 2711 3185 3213 4754 1239
Size: 1391 81 474 28 1541 2579
Fragments arranged by size:
2579 1541 1391 474 81 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4219 5084 5382 4219
Size: 865 298 4931
Fragments arranged by size:
4931 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2240 2801 4757 882
Size: 617 741 561 1956 2219
Fragments arranged by size:
2219 1956 741 617 561
BsaAI yAC'GTr
Cuts at: 493 1204 2050 3885 493
Size: 711 846 1835 2702
Fragments arranged by size:
2702 1835 846 711
BsaBI GATnn'nnATC
Cuts at: 6088 6088
Size: 6094
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3312 3345 3901 4283
Size: 53 83 186 936 1779 33 556 382
Cuts at: 4283 4918 275
Size: 635 1451
Fragments arranged by size:
1779 1451 936 635 556 382 186 83
53 33
BsaWI w'CCGG_w
Cuts at: 2057 4527 4925 5199 5346 5757 2057
Size: 2470 398 274 147 411 2394
Fragments arranged by size:
2470 2394 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2039 2069 1178
Size: 30 165 30 636 30 5203
Fragments arranged by size:
5203 636 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6094
BseYI C'CCAG_C
Cuts at: 2431 5297 5793 2431
Size: 2866 496 2732
Fragments arranged by size:
2866 2732 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5521
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2825 3271 3544 4305 5333 1540
Size: 275 186 824 446 273 761 1028 2301
Fragments arranged by size:
2301 1028 824 761 446 275 273 186
BsiHKAI G_wGCw'C
Cuts at: 4155 4240 5311 4155
Size: 85 1071 4938
Fragments arranged by size:
4938 1071 85
BsmI GAATG_Cn'
Cuts at: 3605 3698 3605
Size: 93 6001
BsmBI CGTCTCn'nnnn_
Cuts at: 3189 3189
Size: 6094
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2571 2580 2616 2793 3413 4155 4240
Size: 399 781 9 36 177 620 742 85
Cuts at: 4240 5311 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 781 742 620 399 177 85
36 9
BspEI T'CCGG_A
Cuts at: 2057 5757 2057
Size: 3700 2394
BspHI T'CATG_A
Cuts at: 3983 5713 3983
Size: 1730 4364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3449 844
Size: 2605 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3339 3981 1394
Size: 429 1516 642 3507
Fragments arranged by size:
3507 1516 642 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4589 4763 65
Size: 1183 3341 174 1396
Fragments arranged by size:
3341 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2482 2810 3068 3144 3347 4738 4925
Size: 110 672 328 258 76 203 1391 187
Cuts at: 4925 1700
Size: 2869
Fragments arranged by size:
2869 1391 672 328 258 203 187 110
76
BsrGI T'GTAC_A
Cuts at: 96 2763 96
Size: 2667 3427
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6094
BssSI C'ACGA_G
Cuts at: 2891 4154 5166 2891
Size: 1263 1012 3819
Fragments arranged by size:
3819 1263 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2416 794
Size: 1622 4472
BstBI TT'CG_AA
Cuts at: 4898 4898
Size: 6094
BstEII G'GTnAC_C
Cuts at: 1184 2836 3355 1184
Size: 1652 519 3923
Fragments arranged by size:
3923 1652 519
BstXI CCAn_nnnn'nTGG
Cuts at: 3238 3238
Size: 6094
BstYI r'GATC_y
Cuts at: 1972 2159 3424 3791 4176 4193 5645 5731 5743
Size: 187 1265 367 385 17 1452 86 12
Cuts at: 5743 6089 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1265 385 367 346 187 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2073 2630 2727 2793 513
Size: 552 939 69 557 97 66 3814
Fragments arranged by size:
3814 939 557 552 97 69 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3091 487
Size: 683 56 1865 3490
Fragments arranged by size:
3490 1865 683 56
BtsI GCAGTG_nn'
Cuts at: 2346 3610 4404 4424 2346
Size: 1264 794 20 4016
Fragments arranged by size:
4016 1264 794 20
ClaI AT'CG_AT
Cuts at: 1554 2274 1554
Size: 720 5374
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2962 2997 529
Size: 35 2398 35 3626
Fragments arranged by size:
3626 2398 35 35
DraI TTT'AAA
Cuts at: 3398 3398
Size: 6094
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2513 5101 809
Size: 507 1197 2588 1802
Fragments arranged by size:
2588 1802 1197 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2044 2284 2559
Size: 54 1425 50 275 18 214 240 275
Cuts at: 2559 2822 3201 3268 3303 3541 4430 6059 8
Size: 263 379 67 35 238 889 1629 43
Fragments arranged by size:
1629 1425 889 379 275 275 263 240
238 214 67 54 50 43 35 18
EagI C'GGCC_G
Cuts at: 1537 1812 2822 3268 3541 1537
Size: 275 1010 446 273 4090
Fragments arranged by size:
4090 1010 446 275 273
EarI CTCTTCn'nnn_
Cuts at: 1353 3329 4024 1353
Size: 1976 695 3423
Fragments arranged by size:
3423 1976 695
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2213 2345 4681 5055 5201 387
Size: 1064 138 624 132 2336 374 146 1280
Fragments arranged by size:
2336 1280 1064 624 374 146 138 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 2630 2711 3149 3185 3206 3213
Size: 393 581 417 81 438 36 21 7
Cuts at: 3213 4157 4754 5541 5809 1239
Size: 944 597 787 268 1524
Fragments arranged by size:
1524 944 787 597 581 438 417 393
268 81 36 21 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2690 2690
Size: 6094
EcoO109I rG'GnC_Cy
Cuts at: 2695 2695
Size: 6094
EcoRI G'AATT_C
Cuts at: 3403 3781 3403
Size: 378 5716
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2179 2179
Size: 6094
FspI TGC'GCA
Cuts at: 2338 4600 2338
Size: 2262 3832
HaeII r_GCGC'y
Cuts at: 1678 2503 2532 3069 3151 3348 5241 1678
Size: 825 29 537 82 197 1893 2531
Fragments arranged by size:
2531 1893 825 537 197 82 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2858 2890 2978 3010 3134
Size: 32 135 32 1286 32 88 32 124
Cuts at: 3134 3166 4735 4767 4809 4841 1373
Size: 32 1569 32 42 32 2626
Fragments arranged by size:
2626 1569 1286 135 124 88 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3438 3684 669
Size: 988 281 1500 246 3079
Fragments arranged by size:
3079 1500 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6094
HpaI GTT'AAC
Cuts at: 3684 3684
Size: 6094
KasI G'GCGC_C
Cuts at: 3344 3344
Size: 6094
KpnI G_GTAC'C
Cuts at: 3419 3419
Size: 6094
MfeI C'AATT_G
Cuts at: 3693 3693
Size: 6094
MluI A'CGCG_T
Cuts at: 5970 5970
Size: 6094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5208 5392 1305
Size: 3903 184 2007
Fragments arranged by size:
3903 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4272
Fragments arranged by size:
4272 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2834 2867 3236 4052 4411 4570 518
Size: 648 1668 33 369 816 359 159 2042
Fragments arranged by size:
2042 1668 816 648 369 359 159 33
MspA1I CmG'CkG
Cuts at: 1606 2752 3510 4187 5335 5580 1606
Size: 1146 758 677 1148 245 2120
Fragments arranged by size:
2120 1148 1146 758 677 245
NaeI GCC'GGC
Cuts at: 2484 3070 3349 2484
Size: 586 279 5229
Fragments arranged by size:
5229 586 279
NarI GG'CG_CC
Cuts at: 3345 3345
Size: 6094
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4603
Fragments arranged by size:
4603 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6094
NgoMIV G'CCGG_C
Cuts at: 2482 3068 3347 2482
Size: 586 279 5229
Fragments arranged by size:
5229 586 279
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6094
NotI GC'GGCC_GC
Cuts at: 3541 3541
Size: 6094
NspI r_CATG'y
Cuts at: 1132 1229 2414 2608 2629 3452 4997 1132
Size: 97 1185 194 21 823 1545 2229
Fragments arranged by size:
2229 1545 1185 823 194 97 21
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6094
PciI A'CATG_T
Cuts at: 4993 4993
Size: 6094
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6094
PmeI GTTT'AAAC
Cuts at: 3398 3398
Size: 6094
PmlI CAC'GTG
Cuts at: 2050 2050
Size: 6094
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4163 4195 5676 5708 4163
Size: 32 1481 32 4549
Fragments arranged by size:
4549 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6094
PsiI TTA'TAA
Cuts at: 3664 3664
Size: 6094
PstI C_TGCA'G
Cuts at: 830 2757 3446 830
Size: 1927 689 3478
Fragments arranged by size:
3478 1927 689
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6094
SalI G'TCGA_C
Cuts at: 1936 3436 1936
Size: 1500 4594
SapI GCTCTTCn'nnn_
Cuts at: 3329 3329
Size: 6094
SbfI CC_TGCA'GG
Cuts at: 3446 3446
Size: 6094
ScaI AGT'ACT
Cuts at: 2258 4342 6054 2258
Size: 2084 1712 2298
Fragments arranged by size:
2298 2084 1712
SfcI C'TryA_G
Cuts at: 826 1045 2488 2753 3442 4577 5258 5449 826
Size: 219 1443 265 689 1135 681 191 1471
Fragments arranged by size:
1471 1443 1135 689 681 265 219 191
SfoI GGC'GCC
Cuts at: 3346 3346
Size: 6094
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6094
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6094
SmaI CCC'GGG
Cuts at: 1872 3054 3421 1872
Size: 1182 367 4545
Fragments arranged by size:
4545 1182 367
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4198 5099 5361 820
Size: 197 926 2255 901 262 1553
Fragments arranged by size:
2255 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3885 493
Size: 3392 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6094
SphI G_CATG'C
Cuts at: 1132 2414 3452 1132
Size: 1282 1038 3774
Fragments arranged by size:
3774 1282 1038
SspI AAT'ATT
Cuts at: 5 52 4018 5
Size: 47 3966 2081
Fragments arranged by size:
3966 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6094
StyI C'CwwG_G
Cuts at: 513 1065 2004 3533 513
Size: 552 939 1529 3074
Fragments arranged by size:
3074 1529 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2167 2423 4121 4138 4291 4476 4921
Size: 1255 587 256 1698 17 153 185 445
Cuts at: 4921 325
Size: 1498
Fragments arranged by size:
1698 1498 1255 587 445 256 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2256 2763 4340
Size: 274 80 33 51 1443 279 507 1577
Cuts at: 4340 6052 96
Size: 1712 138
Fragments arranged by size:
1712 1577 1443 507 279 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2039 2071 6054 6086 2039
Size: 32 3983 32 2047
Fragments arranged by size:
3983 2047 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2924 1316
Size: 1608 4486
XbaI T'CTAG_A
Cuts at: 3430 3430
Size: 6094
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3211 3211
Size: 6094
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6094
XmaI C'CCGG_G
Cuts at: 1870 3052 3419 1870
Size: 1182 367 4545
Fragments arranged by size:
4545 1182 367
XmnI GAAnn'nnTTC
Cuts at: 4223 4223
Size: 6094
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3902 276
Size: 53 83 186 936 2368 2468
Fragments arranged by size:
2468 2368 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI
FspAI NruI NsiI PacI PfoI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI
SrfI SwaI