Restriction map for
pFN21AE3668
(Circular) (Six-base) MAPSORT of: pFN21AE3668.seq Check: 6948 from: 1 to: 6100
pFN21AE3668 6100 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3004 3004
Size: 6100
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3910 278
Size: 53 83 186 936 2374 2468
Fragments arranged by size:
2468 2374 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3128 3443 1937
Size: 1191 315 4594
Fragments arranged by size:
4594 1191 315
Acc65I G'GTAC_C
Cuts at: 3421 3421
Size: 6100
AclI AA'CG_TT
Cuts at: 1563 4227 4600 1563
Size: 2664 373 3063
Fragments arranged by size:
3063 2664 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2114 2239 4163 5547 5815 1632
Size: 482 125 1924 1384 268 1917
Fragments arranged by size:
1924 1917 1384 482 268 125
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5903
AflIII A'CryG_T
Cuts at: 1203 3144 4999 5976 1203
Size: 1941 1855 977 1327
Fragments arranged by size:
1941 1855 1327 977
AgeI A'CCGG_T
Cuts at: 4931 4931
Size: 6100
AhdI GACnn_n'nnGTC
Cuts at: 4829 4829
Size: 6100
AleI CACnn'nnGTG
Cuts at: 1166 2186 1166
Size: 1020 5080
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6100
AlwNI CAG_nnn'CTG
Cuts at: 2062 2866 3843 5415 2062
Size: 804 977 1572 2747
Fragments arranged by size:
2747 1572 977 804
ApaLI G'TGCA_C
Cuts at: 4157 5313 4157
Size: 1156 4944
ApoI r'AATT_y
Cuts at: 1493 3409 3635 3787 5896 1493
Size: 1916 226 152 2109 1697
Fragments arranged by size:
2109 1916 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4654 160
Size: 872 3622 1606
Fragments arranged by size:
3622 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6100
AvaI C'yCGr_G
Cuts at: 1870 1943 2336 3425 1870
Size: 73 393 1089 4545
Fragments arranged by size:
4545 1089 393 73
BamHI G'GATC_C
Cuts at: 3430 3797 3430
Size: 367 5733
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2777 3421 4876 618
Size: 325 217 627 990 644 1455 1842
Fragments arranged by size:
1842 1455 990 644 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2846 3419 1391
Size: 1455 573 4072
Fragments arranged by size:
4072 1455 573
BbeI G_GCGC'C
Cuts at: 2781 2781
Size: 6100
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2315 2481 2658 928
Size: 971 416 166 177 4370
Fragments arranged by size:
4370 971 416 177 166
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2828 2862 4291 4325 1903
Size: 34 50 34 807 34 1429 34 3678
Fragments arranged by size:
3678 1429 807 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3992 5202 3992
Size: 1210 4890
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6100
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4711 136
Size: 107 122 71 631 605 3039 1525
Fragments arranged by size:
3039 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3045 6095 3045
Size: 3050 3050
BlpI GC'TnA_GC
Cuts at: 3517 3517
Size: 6100
Bme1580I G_kGCm'C
Cuts at: 1790 2687 4161 5317 1790
Size: 897 1474 1156 2573
Fragments arranged by size:
2573 1474 1156 897
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6100
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3831 4789 442
Size: 416 53 83 399 24 2414 958 1753
Fragments arranged by size:
2414 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6100
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2410 2410
Size: 6100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2165 2837 4760 1239
Size: 926 672 1923 2579
Fragments arranged by size:
2579 1923 926 672
Bpu10I CC'TnA_GC
Cuts at: 2924 3059 2924
Size: 135 5965
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4225 5090 5388 4225
Size: 865 298 4937
Fragments arranged by size:
4937 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2141 3364 4763 882
Size: 617 642 1223 1399 2219
Fragments arranged by size:
2219 1399 1223 642 617
BsaAI yAC'GTr
Cuts at: 493 1204 2188 3891 493
Size: 711 984 1703 2702
Fragments arranged by size:
2702 1703 984 711
BsaBI GATnn'nnATC
Cuts at: 6094 6094
Size: 6100
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2343 2778 3168 3296
Size: 53 83 186 936 810 435 390 128
Cuts at: 3296 3907 4289 4924 275
Size: 611 382 635 1451
Fragments arranged by size:
1451 936 810 635 611 435 390 382
186 128 83 53
BsaWI w'CCGG_w
Cuts at: 4533 4931 5205 5352 5763 4533
Size: 398 274 147 411 4870
Fragments arranged by size:
4870 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2177 2207 2213 2243 2282
Size: 30 165 30 774 30 6 30 39
Cuts at: 2282 2312 1178
Size: 30 4966
Fragments arranged by size:
4966 774 165 39 30 30 30 30
30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 2303 2726 3344 1186
Size: 913 204 423 618 3942
Fragments arranged by size:
3942 913 618 423 204
BseYI C'CCAG_C
Cuts at: 3067 3254 3299 3391 5303 5799 3067
Size: 187 45 92 1912 496 3368
Fragments arranged by size:
3368 1912 496 187 92 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3233 1163
Size: 573 1497 4030
Fragments arranged by size:
4030 1497 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3037 3550 4311 5339 1540
Size: 275 186 1036 513 761 1028 2301
Fragments arranged by size:
2301 1036 1028 761 513 275 186
BsiHKAI G_wGCw'C
Cuts at: 2846 3065 3152 4161 4246 5317 2846
Size: 219 87 1009 85 1071 3629
Fragments arranged by size:
3629 1071 1009 219 87 85
BsmI GAATG_Cn'
Cuts at: 3611 3704 3611
Size: 93 6007
BsmBI CGTCTCn'nnnn_
Cuts at: 2101 2556 2990 2101
Size: 455 434 5211
Fragments arranged by size:
5211 455 434
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2687 2846 3065 3152 3419 4161 4246
Size: 399 897 159 219 87 267 742 85
Cuts at: 4246 5317 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 897 742 399 267 219 159
87 85
BspEI T'CCGG_A
Cuts at: 5763 5763
Size: 6100
BspHI T'CATG_A
Cuts at: 3989 5719 3989
Size: 1730 4370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2952 3004 3455 844
Size: 2108 52 451 3489
Fragments arranged by size:
3489 2108 451 52
BsrBI CCG'CTC
Cuts at: 1394 1823 3268 3987 1394
Size: 429 1445 719 3507
Fragments arranged by size:
3507 1445 719 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2613 2701 4595 4769 65
Size: 1183 1365 88 1894 174 1396
Fragments arranged by size:
1894 1396 1365 1183 174 88
BsrFI r'CCGG_y
Cuts at: 1700 1810 2351 4744 4931 1700
Size: 110 541 2393 187 2869
Fragments arranged by size:
2869 2393 541 187 110
BsrGI T'GTAC_A
Cuts at: 96 3240 96
Size: 3144 2956
BssHII G'CGCG_C
Cuts at: 1925 3133 1925
Size: 1208 4892
BssSI C'ACGA_G
Cuts at: 2930 4160 5172 2930
Size: 1230 1012 3858
Fragments arranged by size:
3858 1230 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2691 2700 794
Size: 1897 9 4194
Fragments arranged by size:
4194 1897 9
BstBI TT'CG_AA
Cuts at: 4904 4904
Size: 6100
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6100
BstYI r'GATC_y
Cuts at: 1972 2058 3045 3430 3797 4182 4199 5651 5737
Size: 86 987 385 367 385 17 1452 86
Cuts at: 5737 5749 6095 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 987 385 385 367 346 86
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2138 2524 2138
Size: 386 5714
BtgI C'CryG_G
Cuts at: 513 1065 2131 2953 513
Size: 552 1066 822 3660
Fragments arranged by size:
3660 1066 822 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5361
Fragments arranged by size:
5361 683 56
BtsI GCAGTG_nn'
Cuts at: 2303 2355 3343 3616 4410 4430 2303
Size: 52 988 273 794 20 3973
Fragments arranged by size:
3973 988 794 273 52 20
ClaI AT'CG_AT
Cuts at: 1554 2481 1554
Size: 927 5173
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6065
DraI TTT'AAA
Cuts at: 3404 3404
Size: 6100
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5107 809
Size: 507 3791 1802
Fragments arranged by size:
3791 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2625 3340 3547
Size: 54 1425 50 275 18 795 715 207
Cuts at: 3547 4436 6065 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 795 715 275 207 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3547 1537
Size: 275 1735 4090
Fragments arranged by size:
4090 1735 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2151 2375 2954 4030 1353
Size: 798 224 579 1076 3423
Fragments arranged by size:
3423 1076 798 579 224
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4687 5061 5207 387
Size: 1064 138 3098 374 146 1280
Fragments arranged by size:
3098 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2844 2844
Size: 6100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 2165 2239 2837 4163 4760 5547
Size: 393 482 51 74 598 1326 597 787
Cuts at: 5547 5815 1239
Size: 268 1524
Fragments arranged by size:
1524 1326 787 598 597 482 393 268
74 51
EcoNI CCTnn'n_nnAGG
Cuts at: 2096 2096
Size: 6100
EcoO109I rG'GnC_Cy
Cuts at: 2089 2089
Size: 6100
EcoRI G'AATT_C
Cuts at: 3409 3787 3409
Size: 378 5722
FspI TGC'GCA
Cuts at: 2978 3141 3212 3287 4606 2978
Size: 163 71 75 1319 4472
Fragments arranged by size:
4472 1319 163 75 71
FspAI rTGC'GCAy
Cuts at: 3212 3212
Size: 6100
HaeII r_GCGC'y
Cuts at: 1678 2781 5247 1678
Size: 1103 2466 2531
Fragments arranged by size:
2531 2466 1103
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2213 2245 2366 2398 2553
Size: 32 135 32 641 32 121 32 155
Cuts at: 2553 2585 2828 2860 3110 3142 4741 4773 4815
Size: 32 243 32 250 32 1599 32 42
Cuts at: 4815 4847 1373
Size: 32 2626
Fragments arranged by size:
2626 1599 641 250 243 155 135 121
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3444 3690 669
Size: 988 281 1506 246 3079
Fragments arranged by size:
3079 1506 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6100
HpaI GTT'AAC
Cuts at: 3690 3690
Size: 6100
KasI G'GCGC_C
Cuts at: 2777 2777
Size: 6100
KpnI G_GTAC'C
Cuts at: 3425 3425
Size: 6100
MfeI C'AATT_G
Cuts at: 3699 3699
Size: 6100
MluI A'CGCG_T
Cuts at: 5976 5976
Size: 6100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2421 3306 5214 5398 1305
Size: 1116 885 1908 184 2007
Fragments arranged by size:
2007 1908 1116 885 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2627 10
Size: 54 1425 343 795 3483
Fragments arranged by size:
3483 1425 795 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2186 2678 3218 4058 4417 4576 518
Size: 648 1020 492 540 840 359 159 2042
Fragments arranged by size:
2042 1020 840 648 540 492 359 159
MspA1I CmG'CkG
Cuts at: 1606 2077 2133 2866 2974 3303 3516 4193 5341
Size: 471 56 733 108 329 213 677 1148
Cuts at: 5341 5586 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 733 677 471 329 245 213
108 56
NarI GG'CG_CC
Cuts at: 2778 2778
Size: 6100
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5548
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6100
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6100
NotI GC'GGCC_GC
Cuts at: 3547 3547
Size: 6100
NspI r_CATG'y
Cuts at: 1132 1229 2758 3148 3184 3458 5003 1132
Size: 97 1529 390 36 274 1545 2229
Fragments arranged by size:
2229 1545 1529 390 274 97 36
PasI CC'CwG_GG
Cuts at: 1348 2440 1348
Size: 1092 5008
PciI A'CATG_T
Cuts at: 3144 4999 3144
Size: 1855 4245
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6100
PfoI T'CCnGG_A
Cuts at: 2403 2403
Size: 6100
PmeI GTTT'AAAC
Cuts at: 3404 3404
Size: 6100
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4169 4201 5682 5714 4169
Size: 32 1481 32 4555
Fragments arranged by size:
4555 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2089 2089
Size: 6100
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6100
PsiI TTA'TAA
Cuts at: 3670 3670
Size: 6100
PstI C_TGCA'G
Cuts at: 830 2082 3452 830
Size: 1252 1370 3478
Fragments arranged by size:
3478 1370 1252
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6100
PvuII CAG'CTG
Cuts at: 2077 2866 2974 3303 2077
Size: 789 108 329 4874
Fragments arranged by size:
4874 789 329 108
SacI G_AGCT'C
Cuts at: 2846 2846
Size: 6100
SacII CC_GC'GG
Cuts at: 2134 2134
Size: 6100
SalI G'TCGA_C
Cuts at: 1936 3442 1936
Size: 1506 4594
SapI GCTCTTCn'nnn_
Cuts at: 2375 2954 2375
Size: 579 5521
SbfI CC_TGCA'GG
Cuts at: 3452 3452
Size: 6100
ScaI AGT'ACT
Cuts at: 4348 6060 4348
Size: 1712 4388
SfcI C'TryA_G
Cuts at: 826 1045 2078 3448 4583 5264 5455 826
Size: 219 1033 1370 1135 681 191 1471
Fragments arranged by size:
1471 1370 1135 1033 681 219 191
SfoI GGC'GCC
Cuts at: 2779 2779
Size: 6100
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6100
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6100
SmaI CCC'GGG
Cuts at: 1872 3427 1872
Size: 1555 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4204 5105 5367 820
Size: 197 926 2261 901 262 1553
Fragments arranged by size:
2261 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3891 493
Size: 3398 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6100
SphI G_CATG'C
Cuts at: 1132 3184 3458 1132
Size: 2052 274 3774
Fragments arranged by size:
3774 2052 274
SspI AAT'ATT
Cuts at: 5 52 4024 5
Size: 47 3972 2081
Fragments arranged by size:
3972 2081 47
StuI AGG'CCT
Cuts at: 1517 2522 2963 3383 1517
Size: 1005 441 420 4234
Fragments arranged by size:
4234 1005 441 420
StyI C'CwwG_G
Cuts at: 513 1065 2508 3539 513
Size: 552 1443 1031 3074
Fragments arranged by size:
3074 1443 1031 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2231 4127 4144 4297 4482 4927 325
Size: 1255 651 1896 17 153 185 445 1498
Fragments arranged by size:
1896 1498 1255 651 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2325 2892 3240
Size: 274 80 33 51 1443 348 567 348
Cuts at: 3240 4346 6058 96
Size: 1106 1712 138
Fragments arranged by size:
1712 1443 1106 567 348 348 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2282 2314 6060 6092 2282
Size: 32 3746 32 2290
Fragments arranged by size:
3746 2290 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6100
XbaI T'CTAG_A
Cuts at: 3436 3436
Size: 6100
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6100
XmaI C'CCGG_G
Cuts at: 1870 3425 1870
Size: 1555 4545
XmnI GAAnn'nnTTC
Cuts at: 4229 4229
Size: 6100
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3908 276
Size: 53 83 186 936 2374 2468
Fragments arranged by size:
2468 2374 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BsiWI BstXI BstZ17I DraIII EcoRV FalI FseI NaeI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SanDI SexAI SfiI SrfI SwaI XcmI