Restriction map for
pFN21AE3664
(Circular) (Six-base) MAPSORT of: pFN21AE3664.seq Check: 5534 from: 1 to: 6667
pFN21AE3664 6667 bp
With 182 enzymes: SgfI *
March 28, 2012 12:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3548 3567 3868 3548
Size: 19 301 6347
Fragments arranged by size:
6347 301 19
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4477 278
Size: 53 83 186 936 2941 2468
Fragments arranged by size:
2941 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4010 1937
Size: 2073 4594
Acc65I G'GTAC_C
Cuts at: 3988 3988
Size: 6667
AclI AA'CG_TT
Cuts at: 1563 4794 5167 1563
Size: 3231 373 3063
Fragments arranged by size:
3231 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2274 2652 3037 3766 4730 6114 6382 1632
Size: 642 378 385 729 964 1384 268 1917
Fragments arranged by size:
1917 1384 964 729 642 385 378 268
AfeI AGC'GCT
Cuts at: 2459 3050 2459
Size: 591 6076
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6470
AflIII A'CryG_T
Cuts at: 1203 5566 6543 1203
Size: 4363 977 1327
Fragments arranged by size:
4363 1327 977
AgeI A'CCGG_T
Cuts at: 5498 5498
Size: 6667
AhdI GACnn_n'nnGTC
Cuts at: 5396 5396
Size: 6667
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6667
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3599 1184
Size: 2415 4252
AlwNI CAG_nnn'CTG
Cuts at: 2059 2224 2239 2701 4410 5982 2059
Size: 165 15 462 1709 1572 2744
Fragments arranged by size:
2744 1709 1572 462 165 15
ApaI G_GGCC'C
Cuts at: 2386 3356 3475 2386
Size: 970 119 5578
Fragments arranged by size:
5578 970 119
ApaLI G'TGCA_C
Cuts at: 3701 4724 5880 3701
Size: 1023 1156 4488
Fragments arranged by size:
4488 1156 1023
ApoI r'AATT_y
Cuts at: 1493 3976 4202 4354 6463 1493
Size: 2483 226 152 2109 1697
Fragments arranged by size:
2483 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5221 160
Size: 872 4189 1606
Fragments arranged by size:
4189 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6667
AvaI C'yCGr_G
Cuts at: 1870 1943 2184 2265 2367 2504 2682 2737 2836
Size: 73 241 81 102 137 178 55 99
Cuts at: 2836 2977 3522 3952 3992 1870
Size: 141 545 430 40 4545
Fragments arranged by size:
4545 545 430 241 178 141 137 102
99 81 73 55 40
AvrII C'CTAG_G
Cuts at: 2084 3649 2084
Size: 1565 5102
BamHI G'GATC_C
Cuts at: 3997 4364 3997
Size: 367 6300
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3575 3988 5443 618
Size: 325 217 627 1788 413 1455 1842
Fragments arranged by size:
1842 1788 1455 627 413 325 217
BanII G_rGCy'C
Cuts at: 1391 2386 2775 2984 3356 3475 3501 3966 3986
Size: 995 389 209 372 119 26 465 20
Cuts at: 3986 1391
Size: 4072
Fragments arranged by size:
4072 995 465 389 372 209 119 26
20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3411 928
Size: 971 1512 4184
Fragments arranged by size:
4184 1512 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4858 4892 1903
Size: 34 2921 34 3678
Fragments arranged by size:
3678 2921 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4559 5769 4559
Size: 1210 5457
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6667
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3651 5278 136
Size: 107 122 71 631 605 1979 1627 1525
Fragments arranged by size:
1979 1627 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6662 6662
Size: 6667
BlpI GC'TnA_GC
Cuts at: 2702 4084 2702
Size: 1382 5285
Bme1580I G_kGCm'C
Cuts at: 1790 2386 3073 3089 3356 3475 3578 3705 4728
Size: 596 687 16 267 119 103 127 1023
Cuts at: 4728 5884 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1023 687 596 267 127 119
103 16
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6667
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2800 3092 3382
Size: 416 53 83 399 24 1383 292 290
Cuts at: 3382 3604 3945 4398 5356 442
Size: 222 341 453 958 1753
Fragments arranged by size:
1753 1383 958 453 416 399 341 292
290 222 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6667
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2188 2188
Size: 6667
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3626 5327 1239
Size: 2387 1701 2579
Fragments arranged by size:
2579 2387 1701
Bpu10I CC'TnA_GC
Cuts at: 2106 2106
Size: 6667
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4792 5657 5955 4792
Size: 865 298 5504
Fragments arranged by size:
5504 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2299 2740 5330 882
Size: 617 800 441 2590 2219
Fragments arranged by size:
2590 2219 800 617 441
BsaAI yAC'GTr
Cuts at: 493 1204 2614 4458 493
Size: 711 1410 1844 2702
Fragments arranged by size:
2702 1844 1410 711
BsaBI GATnn'nnATC
Cuts at: 6661 6661
Size: 6667
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3127 4474 4856 5491
Size: 53 83 186 936 1594 1347 382 635
Cuts at: 5491 275
Size: 1451
Fragments arranged by size:
1594 1451 1347 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5100 5498 5772 5919 6330 5100
Size: 398 274 147 411 5437
Fragments arranged by size:
5437 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2945 2975 3925 3955 1178
Size: 30 165 30 1542 30 950 30 3890
Fragments arranged by size:
3890 1542 950 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2144 1186
Size: 856 102 5709
Fragments arranged by size:
5709 856 102
BseYI C'CCAG_C
Cuts at: 2070 2179 2454 2460 2636 3094 3223 3288 3365
Size: 109 275 6 176 458 129 65 77
Cuts at: 3365 3383 3571 3641 3711 3802 5870 6366 2070
Size: 18 188 70 70 91 2068 496 2371
Fragments arranged by size:
2371 2068 496 458 275 188 176 129
109 91 77 70 70 65 18 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2210 2290 3095 1163
Size: 573 474 80 805 4735
Fragments arranged by size:
4735 805 573 474 80
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2543 4117 4878 5906 1540
Size: 275 186 542 1574 761 1028 2301
Fragments arranged by size:
2301 1574 1028 761 542 275 186
BsiHKAI G_wGCw'C
Cuts at: 2093 3624 3705 3861 4728 4813 5884 2093
Size: 1531 81 156 867 85 1071 2876
Fragments arranged by size:
2876 1531 1071 867 156 85 81
BsmI GAATG_Cn'
Cuts at: 4178 4271 4178
Size: 93 6574
BsmBI CGTCTCn'nnnn_
Cuts at: 2736 3466 2736
Size: 730 5937
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2093 2386 2775 2984 3073 3089 3356
Size: 399 303 293 389 209 89 16 267
Cuts at: 3356 3475 3501 3578 3624 3705 3861 3966 3986
Size: 119 26 77 46 81 156 105 20
Cuts at: 3986 4728 4813 5884 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 389 303 293 267
209 156 119 105 89 85 81 77
46 26 20 16
BspEI T'CCGG_A
Cuts at: 6330 6330
Size: 6667
BspHI T'CATG_A
Cuts at: 4556 6286 4556
Size: 1730 4937
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2538 2964 3166 3279 3548 3567 3868 4022
Size: 1694 426 202 113 269 19 301 154
Cuts at: 4022 844
Size: 3489
Fragments arranged by size:
3489 1694 426 301 269 202 154 113
19
BsrBI CCG'CTC
Cuts at: 1394 1823 2152 3841 4554 1394
Size: 429 329 1689 713 3507
Fragments arranged by size:
3507 1689 713 429 329
BsrDI GCAATG_nn'
Cuts at: 65 1248 5162 5336 65
Size: 1183 3914 174 1396
Fragments arranged by size:
3914 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2373 5311 5498 1700
Size: 110 563 2938 187 2869
Fragments arranged by size:
2938 2869 563 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6667
BssHII G'CGCG_C
Cuts at: 1925 3680 1925
Size: 1755 4912
BssSI C'ACGA_G
Cuts at: 3189 4727 5739 3189
Size: 1538 1012 4117
Fragments arranged by size:
4117 1538 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2224 794
Size: 1430 5237
BstBI TT'CG_AA
Cuts at: 5471 5471
Size: 6667
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6667
BstYI r'GATC_y
Cuts at: 1972 3997 4364 4749 4766 6218 6304 6316 6662
Size: 2025 367 385 17 1452 86 12 346
Cuts at: 6662 1972
Size: 1977
Fragments arranged by size:
2025 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2077 2112 2158 3121 3234 3762 3813
Size: 552 1012 35 46 963 113 528 51
Cuts at: 3813 513
Size: 3367
Fragments arranged by size:
3367 1012 963 552 528 113 51 46
35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2199 487
Size: 683 56 973 4955
Fragments arranged by size:
4955 973 683 56
BtsI GCAGTG_nn'
Cuts at: 2466 2498 2603 2865 3073 3107 3410 3926 4183
Size: 32 105 262 208 34 303 516 257
Cuts at: 4183 4977 4997 2466
Size: 794 20 4136
Fragments arranged by size:
4136 794 516 303 262 257 208 105
34 32 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6667
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6632
DraI TTT'AAA
Cuts at: 3971 3971
Size: 6667
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5674 809
Size: 507 4358 1802
Fragments arranged by size:
4358 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2286 2540 2850
Size: 54 1425 50 275 18 456 254 310
Cuts at: 2850 2941 3630 3759 4114 5003 6632 8
Size: 91 689 129 355 889 1629 43
Fragments arranged by size:
1629 1425 889 689 456 355 310 275
254 129 91 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2540 4114 1537
Size: 275 728 1574 4090
Fragments arranged by size:
4090 1574 728 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2430 3282 4597 1353
Size: 1077 852 1315 3423
Fragments arranged by size:
3423 1315 1077 852
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3818 5254 5628 5774 387
Size: 1064 138 2229 1436 374 146 1280
Fragments arranged by size:
2229 1436 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2274 2652 3037 3626 3766 4730 5327
Size: 393 642 378 385 589 140 964 597
Cuts at: 5327 6114 6382 1239
Size: 787 268 1524
Fragments arranged by size:
1524 964 787 642 597 589 393 385
378 268 140
EcoO109I rG'GnC_Cy
Cuts at: 2221 2262 2382 2584 3057 3352 3471 3472 3531
Size: 41 120 202 473 295 119 1 59
Cuts at: 3531 2221
Size: 5357
Fragments arranged by size:
5357 473 295 202 120 119 59 41
1
EcoRI G'AATT_C
Cuts at: 3976 4354 3976
Size: 378 6289
EcoRV GAT'ATC
Cuts at: 3037 3037
Size: 6667
FspI TGC'GCA
Cuts at: 5173 5173
Size: 6667
HaeII r_GCGC'y
Cuts at: 1678 2461 3052 3657 5814 1678
Size: 783 591 605 2157 2531
Fragments arranged by size:
2531 2157 783 605 591
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2945 2977 5308 5340 5382
Size: 32 135 32 1373 32 2331 32 42
Cuts at: 5382 5414 1373
Size: 32 2626
Fragments arranged by size:
2626 2331 1373 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4011 4257 669
Size: 988 281 2073 246 3079
Fragments arranged by size:
3079 2073 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6667
HpaI GTT'AAC
Cuts at: 4257 4257
Size: 6667
KpnI G_GTAC'C
Cuts at: 3992 3992
Size: 6667
MfeI C'AATT_G
Cuts at: 4266 4266
Size: 6667
MluI A'CGCG_T
Cuts at: 6543 6543
Size: 6667
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3180 5781 5965 1305
Size: 1875 2601 184 2007
Fragments arranged by size:
2601 2007 1875 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2943 3632 3761 10
Size: 54 1425 343 1111 689 129 2916
Fragments arranged by size:
2916 1425 1111 689 343 129 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4625 4984 5143 518
Size: 648 3459 359 159 2042
Fragments arranged by size:
3459 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2236 2339 2988 3123 3397 3571 3583 3802
Size: 630 103 649 135 274 174 12 219
Cuts at: 3802 3815 4083 4760 5908 6153 1606
Size: 13 268 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 649 630 274 268 245
219 174 135 103 13 12
NaeI GCC'GGC
Cuts at: 2375 2375
Size: 6667
NcoI C'CATG_G
Cuts at: 513 1065 2112 2158 3234 3762 513
Size: 552 1047 46 1076 528 3418
Fragments arranged by size:
3418 1076 1047 552 528 46
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6667
NgoMIV G'CCGG_C
Cuts at: 2373 2373
Size: 6667
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6667
NotI GC'GGCC_GC
Cuts at: 4114 4114
Size: 6667
NspI r_CATG'y
Cuts at: 1132 1229 3680 4025 5570 1132
Size: 97 2451 345 1545 2229
Fragments arranged by size:
2451 2229 1545 345 97
PasI CC'CwG_GG
Cuts at: 1348 2890 1348
Size: 1542 5125
PciI A'CATG_T
Cuts at: 5566 5566
Size: 6667
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2950 3494 1108
Size: 1842 544 4281
Fragments arranged by size:
4281 1842 544
PmeI GTTT'AAAC
Cuts at: 3971 3971
Size: 6667
PmlI CAC'GTG
Cuts at: 2614 2614
Size: 6667
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4736 4768 6249 6281 4736
Size: 32 1481 32 5122
Fragments arranged by size:
5122 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6667
PsiI TTA'TAA
Cuts at: 4237 4237
Size: 6667
PspOMI G'GGCC_C
Cuts at: 2382 3352 3471 2382
Size: 970 119 5578
Fragments arranged by size:
5578 970 119
PstI C_TGCA'G
Cuts at: 830 4019 830
Size: 3189 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6667
PvuII CAG'CTG
Cuts at: 3802 3802
Size: 6667
SacII CC_GC'GG
Cuts at: 3124 3816 3124
Size: 692 5975
SalI G'TCGA_C
Cuts at: 1936 4009 1936
Size: 2073 4594
SbfI CC_TGCA'GG
Cuts at: 4019 4019
Size: 6667
ScaI AGT'ACT
Cuts at: 4915 6627 4915
Size: 1712 4955
SexAI A'CCwGG_T
Cuts at: 3660 3660
Size: 6667
SfcI C'TryA_G
Cuts at: 826 1045 3896 4015 5150 5831 6022 826
Size: 219 2851 119 1135 681 191 1471
Fragments arranged by size:
2851 1471 1135 681 219 191 119
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6667
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6667
SmaI CCC'GGG
Cuts at: 1872 2267 2506 2684 3524 3954 3994 1872
Size: 395 239 178 840 430 40 4545
Fragments arranged by size:
4545 840 430 395 239 178 40
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4771 5672 5934 820
Size: 197 926 2828 901 262 1553
Fragments arranged by size:
2828 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4458 493
Size: 3965 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6667
SphI G_CATG'C
Cuts at: 1132 3680 4025 1132
Size: 2548 345 3774
Fragments arranged by size:
3774 2548 345
SspI AAT'ATT
Cuts at: 5 52 4591 5
Size: 47 4539 2081
Fragments arranged by size:
4539 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6667
StyI C'CwwG_G
Cuts at: 513 1065 2084 2112 2158 3060 3234 3438 3649
Size: 552 1019 28 46 902 174 204 211
Cuts at: 3649 3762 4106 513
Size: 113 344 3074
Fragments arranged by size:
3074 1019 902 552 344 211 204 174
113 46 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2961 3586 3703 4694 4711 4864 5049
Size: 1255 1381 625 117 991 17 153 185
Cuts at: 5049 5494 325
Size: 445 1498
Fragments arranged by size:
1498 1381 1255 991 625 445 185 153
117 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4913 6625 96
Size: 274 80 33 51 1443 2936 1712 138
Fragments arranged by size:
2936 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3925 3957 6627 6659 3925
Size: 32 2670 32 3933
Fragments arranged by size:
3933 2670 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6667
XbaI T'CTAG_A
Cuts at: 4003 4003
Size: 6667
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2165 2165
Size: 6667
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6667
XmaI C'CCGG_G
Cuts at: 1870 2265 2504 2682 3522 3952 3992 1870
Size: 395 239 178 840 430 40 4545
Fragments arranged by size:
4545 840 430 395 239 178 40
XmnI GAAnn'nnTTC
Cuts at: 4796 4796
Size: 6667
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4475 276
Size: 53 83 186 936 2941 2468
Fragments arranged by size:
2941 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BbvCI BfrBI BsiWI BstXI
BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI
KasI NarI NruI NsiI PacI PfoI PpuMI PspXI
PsrI RsrII SacI SanDI SapI SfiI SfoI SrfI
SwaI