Restriction map for
pFN21AE3663
(Circular) (Six-base) MAPSORT of: pFN21AE3663.seq Check: 4713 from: 1 to: 5293
pFN21AE3663 5293 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3103 278
Size: 53 83 186 936 1567 2468
Fragments arranged by size:
2468 1567 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2636 1937
Size: 699 4594
Acc65I G'GTAC_C
Cuts at: 2614 2614
Size: 5293
AclI AA'CG_TT
Cuts at: 1563 3420 3793 1563
Size: 1857 373 3063
Fragments arranged by size:
3063 1857 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3356 4740 5008 1632
Size: 1724 1384 268 1917
Fragments arranged by size:
1917 1724 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5096
AflIII A'CryG_T
Cuts at: 1203 4192 5169 1203
Size: 2989 977 1327
Fragments arranged by size:
2989 1327 977
AgeI A'CCGG_T
Cuts at: 4124 4124
Size: 5293
AhdI GACnn_n'nnGTC
Cuts at: 4022 4022
Size: 5293
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5293
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5293
AlwNI CAG_nnn'CTG
Cuts at: 3036 4608 3036
Size: 1572 3721
ApaI G_GGCC'C
Cuts at: 2222 2222
Size: 5293
ApaLI G'TGCA_C
Cuts at: 2309 3350 4506 2309
Size: 1041 1156 3096
Fragments arranged by size:
3096 1156 1041
ApoI r'AATT_y
Cuts at: 1493 2602 2828 2980 5089 1493
Size: 1109 226 152 2109 1697
Fragments arranged by size:
2109 1697 1109 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3847 160
Size: 872 2815 1606
Fragments arranged by size:
2815 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5293
AvaI C'yCGr_G
Cuts at: 1870 1943 2214 2618 1870
Size: 73 271 404 4545
Fragments arranged by size:
4545 404 271 73
BamHI G'GATC_C
Cuts at: 2452 2623 2990 2452
Size: 171 367 4755
Fragments arranged by size:
4755 367 171
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2189 2209 2245 2614 4069
Size: 325 217 627 402 20 36 369 1455
Cuts at: 4069 618
Size: 1842
Fragments arranged by size:
1842 1455 627 402 369 325 217 36
20
BanII G_rGCy'C
Cuts at: 1391 2161 2222 2289 2612 1391
Size: 770 61 67 323 4072
Fragments arranged by size:
4072 770 323 67 61
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2348 928
Size: 971 449 3873
Fragments arranged by size:
3873 971 449
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3484 3518 1903
Size: 34 1547 34 3678
Fragments arranged by size:
3678 1547 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2348 3185 4395 2348
Size: 837 1210 3246
Fragments arranged by size:
3246 1210 837
BclI T'GATC_A
Cuts at: 1548 2496 1548
Size: 948 4345
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3904 136
Size: 107 122 71 631 605 2232 1525
Fragments arranged by size:
2232 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5288 5288
Size: 5293
BlpI GC'TnA_GC
Cuts at: 2710 2710
Size: 5293
Bme1580I G_kGCm'C
Cuts at: 1790 2214 2222 2313 3354 4510 1790
Size: 424 8 91 1041 1156 2573
Fragments arranged by size:
2573 1156 1041 424 91 8
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5293
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2164 3024 3982
Size: 416 53 83 399 24 747 860 958
Cuts at: 3982 442
Size: 1753
Fragments arranged by size:
1753 958 860 747 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5293
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3953 1239
Size: 2714 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3418 4283 4581 3418
Size: 865 298 4130
Fragments arranged by size:
4130 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2052 3956 882
Size: 617 553 1904 2219
Fragments arranged by size:
2219 1904 617 553
BsaAI yAC'GTr
Cuts at: 493 1204 2359 3084 493
Size: 711 1155 725 2702
Fragments arranged by size:
2702 1155 725 711
BsaBI GATnn'nnATC
Cuts at: 5287 5287
Size: 5293
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3100 3482 4117 275
Size: 53 83 186 936 1567 382 635 1451
Fragments arranged by size:
1567 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3726 4124 4398 4545 4956 3726
Size: 398 274 147 411 4063
Fragments arranged by size:
4063 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5068
Fragments arranged by size:
5068 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2074 2164 2342 1186
Size: 888 90 178 4137
Fragments arranged by size:
4137 888 178 90
BseYI C'CCAG_C
Cuts at: 4496 4992 4496
Size: 496 4797
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4720
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2743 3504 4532 1540
Size: 275 186 742 761 1028 2301
Fragments arranged by size:
2301 1028 761 742 275 186
BsiHKAI G_wGCw'C
Cuts at: 2313 3354 3439 4510 2313
Size: 1041 85 1071 3096
Fragments arranged by size:
3096 1071 1041 85
BsmI GAATG_Cn'
Cuts at: 2804 2897 2804
Size: 93 5200
BsmBI CGTCTCn'nnnn_
Cuts at: 2174 2174
Size: 5293
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2161 2214 2222 2289 2313 2612 3354
Size: 399 371 53 8 67 24 299 742
Cuts at: 3354 3439 4510 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 371 299 85 67
53 24 8
BspEI T'CCGG_A
Cuts at: 4956 4956
Size: 5293
BspHI T'CATG_A
Cuts at: 3182 4912 3182
Size: 1730 3563
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2648 844
Size: 1804 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3180 1394
Size: 429 1357 3507
Fragments arranged by size:
3507 1357 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3788 3962 65
Size: 1183 2540 174 1396
Fragments arranged by size:
2540 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3937 4124 1700
Size: 110 2127 187 2869
Fragments arranged by size:
2869 2127 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5293
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5293
BssSI C'ACGA_G
Cuts at: 3353 4365 3353
Size: 1012 4281
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5293
BstBI TT'CG_AA
Cuts at: 4097 4097
Size: 5293
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5293
BstYI r'GATC_y
Cuts at: 1972 2452 2623 2990 3375 3392 4844 4930 4942
Size: 480 171 367 385 17 1452 86 12
Cuts at: 4942 5288 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 480 385 367 346 171 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2195 2195
Size: 5293
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3802
Fragments arranged by size:
3802 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4554
Fragments arranged by size:
4554 683 56
BtsI GCAGTG_nn'
Cuts at: 2223 2809 3603 3623 2223
Size: 586 794 20 3893
Fragments arranged by size:
3893 794 586 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5293
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5258
DraI TTT'AAA
Cuts at: 2597 2597
Size: 5293
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4300 809
Size: 507 2984 1802
Fragments arranged by size:
2984 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2183 2740 3629
Size: 54 1425 50 275 18 353 557 889
Cuts at: 3629 5258 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 557 353 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2740 1537
Size: 275 928 4090
Fragments arranged by size:
4090 928 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2376 3223 1353
Size: 1023 847 3423
Fragments arranged by size:
3423 1023 847
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2167 3880 4254 4400 387
Size: 1064 138 578 1713 374 146 1280
Fragments arranged by size:
1713 1280 1064 578 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3356 3953 4740 5008 1239
Size: 393 1724 597 787 268 1524
Fragments arranged by size:
1724 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2218 2219 2262 2515 2218
Size: 1 43 253 4996
Fragments arranged by size:
4996 253 43 1
EcoRI G'AATT_C
Cuts at: 2602 2980 2602
Size: 378 4915
FspI TGC'GCA
Cuts at: 3799 3799
Size: 5293
HaeII r_GCGC'y
Cuts at: 1678 4440 1678
Size: 2762 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3934 3966 4008 4040 1373
Size: 32 135 32 2362 32 42 32 2626
Fragments arranged by size:
2626 2362 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2637 2883 669
Size: 988 281 699 246 3079
Fragments arranged by size:
3079 988 699 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5293
HpaI GTT'AAC
Cuts at: 2883 2883
Size: 5293
KpnI G_GTAC'C
Cuts at: 2618 2618
Size: 5293
MfeI C'AATT_G
Cuts at: 2892 2892
Size: 5293
MluI A'CGCG_T
Cuts at: 5169 5169
Size: 5293
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4407 4591 1305
Size: 3102 184 2007
Fragments arranged by size:
3102 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2185 10
Size: 54 1425 343 353 3118
Fragments arranged by size:
3118 1425 353 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3251 3610 3769 518
Size: 648 2085 359 159 2042
Fragments arranged by size:
2085 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2709 3386 4534 4779 1606
Size: 1103 677 1148 245 2120
Fragments arranged by size:
2120 1148 1103 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3802
Fragments arranged by size:
3802 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5293
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5293
NotI GC'GGCC_GC
Cuts at: 2740 2740
Size: 5293
NspI r_CATG'y
Cuts at: 1132 1229 2651 4196 1132
Size: 97 1422 1545 2229
Fragments arranged by size:
2229 1545 1422 97
PasI CC'CwG_GG
Cuts at: 1348 2279 1348
Size: 931 4362
PciI A'CATG_T
Cuts at: 4192 4192
Size: 5293
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5293
PfoI T'CCnGG_A
Cuts at: 2067 2067
Size: 5293
PmeI GTTT'AAAC
Cuts at: 2597 2597
Size: 5293
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3362 3394 4875 4907 3362
Size: 32 1481 32 3748
Fragments arranged by size:
3748 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2262 2515 2262
Size: 253 5040
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5293
PsiI TTA'TAA
Cuts at: 2863 2863
Size: 5293
PspOMI G'GGCC_C
Cuts at: 2218 2218
Size: 5293
PstI C_TGCA'G
Cuts at: 830 2446 2645 830
Size: 1616 199 3478
Fragments arranged by size:
3478 1616 199
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5293
SalI G'TCGA_C
Cuts at: 1936 2635 1936
Size: 699 4594
SbfI CC_TGCA'GG
Cuts at: 2645 2645
Size: 5293
ScaI AGT'ACT
Cuts at: 3541 5253 3541
Size: 1712 3581
SfcI C'TryA_G
Cuts at: 826 1045 2442 2641 3776 4457 4648 826
Size: 219 1397 199 1135 681 191 1471
Fragments arranged by size:
1471 1397 1135 681 219 199 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5293
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5293
SmaI CCC'GGG
Cuts at: 1872 2216 2620 1872
Size: 344 404 4545
Fragments arranged by size:
4545 404 344
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3397 4298 4560 820
Size: 197 926 1454 901 262 1553
Fragments arranged by size:
1553 1454 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3084 493
Size: 2591 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5293
SphI G_CATG'C
Cuts at: 1132 2651 1132
Size: 1519 3774
SspI AAT'ATT
Cuts at: 5 52 3217 5
Size: 47 3165 2081
Fragments arranged by size:
3165 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5293
StyI C'CwwG_G
Cuts at: 513 1065 2004 2732 513
Size: 552 939 728 3074
Fragments arranged by size:
3074 939 728 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3320 3337 3490 3675 4120 325
Size: 1255 1740 17 153 185 445 1498
Fragments arranged by size:
1740 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3539 5251 96
Size: 274 80 33 51 1443 1562 1712 138
Fragments arranged by size:
1712 1562 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2284 2316 5253 5285 2284
Size: 32 2937 32 2292
Fragments arranged by size:
2937 2292 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5293
XbaI T'CTAG_A
Cuts at: 2629 2629
Size: 5293
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5293
XmaI C'CCGG_G
Cuts at: 1870 2214 2618 1870
Size: 344 404 4545
Fragments arranged by size:
4545 404 344
XmnI GAAnn'nnTTC
Cuts at: 3422 3422
Size: 5293
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3101 276
Size: 53 83 186 936 1567 2468
Fragments arranged by size:
2468 1567 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI