Restriction map for
pFN21AE3653
(Circular) (Six-base) MAPSORT of: pFN21AE3653.seq Check: 3298 from: 1 to: 5584
pFN21AE3653 5584 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3394 278
Size: 53 83 186 936 1858 2468
Fragments arranged by size:
2468 1858 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2826 2927 1937
Size: 889 101 4594
Fragments arranged by size:
4594 889 101
Acc65I G'GTAC_C
Cuts at: 2905 2905
Size: 5584
AclI AA'CG_TT
Cuts at: 1563 3711 4084 1563
Size: 2148 373 3063
Fragments arranged by size:
3063 2148 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3647 5031 5299 1632
Size: 2015 1384 268 1917
Fragments arranged by size:
2015 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5387
AflIII A'CryG_T
Cuts at: 1203 4483 5460 1203
Size: 3280 977 1327
Fragments arranged by size:
3280 1327 977
AgeI A'CCGG_T
Cuts at: 4415 4415
Size: 5584
AhdI GACnn_n'nnGTC
Cuts at: 2179 2822 4313 2179
Size: 643 1491 3450
Fragments arranged by size:
3450 1491 643
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5584
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5584
AlwNI CAG_nnn'CTG
Cuts at: 2152 2311 3327 4899 2152
Size: 159 1016 1572 2837
Fragments arranged by size:
2837 1572 1016 159
ApaLI G'TGCA_C
Cuts at: 2597 3641 4797 2597
Size: 1044 1156 3384
Fragments arranged by size:
3384 1156 1044
ApoI r'AATT_y
Cuts at: 1493 2490 2893 3119 3271 5380 1493
Size: 997 403 226 152 2109 1697
Fragments arranged by size:
2109 1697 997 403 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4138 160
Size: 872 3106 1606
Fragments arranged by size:
3106 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5584
AvaI C'yCGr_G
Cuts at: 1870 1943 2668 2909 1870
Size: 73 725 241 4545
Fragments arranged by size:
4545 725 241 73
BamHI G'GATC_C
Cuts at: 2213 2856 2914 3281 2213
Size: 643 58 367 4516
Fragments arranged by size:
4516 643 367 58
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2905 4360 618
Size: 325 217 627 1118 1455 1842
Fragments arranged by size:
1842 1455 1118 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2591 2903 1391
Size: 1200 312 4072
Fragments arranged by size:
4072 1200 312
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2178 928
Size: 971 279 4334
Fragments arranged by size:
4334 971 279
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3775 3809 1903
Size: 34 1838 34 3678
Fragments arranged by size:
3678 1838 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3476 4686 3476
Size: 1210 4374
BclI T'GATC_A
Cuts at: 1548 2049 1548
Size: 501 5083
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4195 136
Size: 107 122 71 631 605 2523 1525
Fragments arranged by size:
2523 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5579 5579
Size: 5584
BlpI GC'TnA_GC
Cuts at: 3001 3001
Size: 5584
Bme1580I G_kGCm'C
Cuts at: 1790 2133 2601 2806 3645 4801 1790
Size: 343 468 205 839 1156 2573
Fragments arranged by size:
2573 1156 839 468 343 205
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5584
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3315 4273 442
Size: 416 53 83 399 24 1898 958 1753
Fragments arranged by size:
1898 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5584
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2372 2372
Size: 5584
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2600 4244 1239
Size: 1361 1644 2579
Fragments arranged by size:
2579 1644 1361
Bpu10I CC'TnA_GC
Cuts at: 2261 2261
Size: 5584
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3709 4574 4872 3709
Size: 865 298 4421
Fragments arranged by size:
4421 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4247 882
Size: 617 2748 2219
Fragments arranged by size:
2748 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3375 493
Size: 711 2171 2702
Fragments arranged by size:
2702 2171 711
BsaBI GATnn'nnATC
Cuts at: 5578 5578
Size: 5584
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2175 3391 3773 4408
Size: 53 83 186 936 642 1216 382 635
Cuts at: 4408 275
Size: 1451
Fragments arranged by size:
1451 1216 936 642 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4017 4415 4689 4836 5247 4017
Size: 398 274 147 411 4354
Fragments arranged by size:
4354 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5359
Fragments arranged by size:
5359 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2518 1186
Size: 1332 4252
BseYI C'CCAG_C
Cuts at: 2159 2235 2546 2571 4787 5283 2159
Size: 76 311 25 2216 496 2460
Fragments arranged by size:
2460 2216 496 311 76 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5011
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2183 2322 3034 3795 4823 1540
Size: 275 186 182 139 712 761 1028 2301
Fragments arranged by size:
2301 1028 761 712 275 186 182 139
BsiHKAI G_wGCw'C
Cuts at: 2040 2047 2601 3645 3730 4801 2040
Size: 7 554 1044 85 1071 2823
Fragments arranged by size:
2823 1071 1044 554 85 7
BsmI GAATG_Cn'
Cuts at: 3095 3188 3095
Size: 93 5491
BsmBI CGTCTCn'nnnn_
Cuts at: 2360 2530 2360
Size: 170 5414
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2047 2133 2591 2601 2806 2903
Size: 399 250 7 86 458 10 205 97
Cuts at: 2903 3645 3730 4801 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 458 399 250 205 97
86 85 10 7
BspEI T'CCGG_A
Cuts at: 5247 5247
Size: 5584
BspHI T'CATG_A
Cuts at: 3473 5203 3473
Size: 1730 3854
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2702 2939 844
Size: 1858 237 3489
Fragments arranged by size:
3489 1858 237
BsrBI CCG'CTC
Cuts at: 1394 1823 3471 1394
Size: 429 1648 3507
Fragments arranged by size:
3507 1648 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2764 4079 4253 65
Size: 1183 1516 1315 174 1396
Fragments arranged by size:
1516 1396 1315 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2699 4228 4415 1700
Size: 110 889 1529 187 2869
Fragments arranged by size:
2869 1529 889 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5584
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5584
BssSI C'ACGA_G
Cuts at: 2777 3644 4656 2777
Size: 867 1012 3705
Fragments arranged by size:
3705 1012 867
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5584
BstBI TT'CG_AA
Cuts at: 4388 4388
Size: 5584
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5584
BstYI r'GATC_y
Cuts at: 1972 2213 2856 2914 3281 3666 3683 5135 5221
Size: 241 643 58 367 385 17 1452 86
Cuts at: 5221 5233 5579 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 643 385 367 346 241 86
58 17 12
Bsu36I CC'TnA_GG
Cuts at: 2607 2607
Size: 5584
BtgI C'CryG_G
Cuts at: 513 1065 2004 2240 513
Size: 552 939 236 3857
Fragments arranged by size:
3857 939 552 236
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4845
Fragments arranged by size:
4845 683 56
BtsI GCAGTG_nn'
Cuts at: 2326 3100 3894 3914 2326
Size: 774 794 20 3996
Fragments arranged by size:
3996 794 774 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5584
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5549
DraI TTT'AAA
Cuts at: 2888 2888
Size: 5584
DraIII CAC_nnn'GTG
Cuts at: 2853 2853
Size: 5584
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4591 809
Size: 507 3275 1802
Fragments arranged by size:
3275 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2319 2359 2449
Size: 54 1425 50 275 18 489 40 90
Cuts at: 2449 3031 3920 5549 8
Size: 582 889 1629 43
Fragments arranged by size:
1629 1425 889 582 489 275 90 54
50 43 40 18
EagI C'GGCC_G
Cuts at: 1537 1812 2319 3031 1537
Size: 275 507 712 4090
Fragments arranged by size:
4090 712 507 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3514 1353
Size: 2161 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4171 4545 4691 387
Size: 1064 138 2582 374 146 1280
Fragments arranged by size:
2582 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2600 3647 4244 5031 5299 1239
Size: 393 968 1047 597 787 268 1524
Fragments arranged by size:
1524 1047 968 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2149 2611 2744 2149
Size: 462 133 4989
Fragments arranged by size:
4989 462 133
EcoRI G'AATT_C
Cuts at: 2490 2893 3271 2490
Size: 403 378 4803
Fragments arranged by size:
4803 403 378
FspI TGC'GCA
Cuts at: 4090 4090
Size: 5584
HaeII r_GCGC'y
Cuts at: 1678 4731 1678
Size: 3053 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2102 2134 2165 2197 2808
Size: 32 135 32 530 32 31 32 611
Cuts at: 2808 2840 4225 4257 4299 4331 1373
Size: 32 1385 32 42 32 2626
Fragments arranged by size:
2626 1385 611 530 135 42 32 32
32 32 32 32 32 31
HincII GTy'rAC
Cuts at: 669 1657 1938 2928 3174 669
Size: 988 281 990 246 3079
Fragments arranged by size:
3079 990 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5584
HpaI GTT'AAC
Cuts at: 3174 3174
Size: 5584
KpnI G_GTAC'C
Cuts at: 2909 2909
Size: 5584
MfeI C'AATT_G
Cuts at: 3183 3183
Size: 5584
MluI A'CGCG_T
Cuts at: 5460 5460
Size: 5584
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2163 2241 4698 4882 1305
Size: 858 78 2457 184 2007
Fragments arranged by size:
2457 2007 858 184 78
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2451 10
Size: 54 1425 343 619 3143
Fragments arranged by size:
3143 1425 619 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3542 3901 4060 518
Size: 648 2376 359 159 2042
Fragments arranged by size:
2376 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3000 3677 4825 5070 1606
Size: 1394 677 1148 245 2120
Fragments arranged by size:
2120 1394 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4093
Fragments arranged by size:
4093 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5584
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5584
NotI GC'GGCC_GC
Cuts at: 3031 3031
Size: 5584
NspI r_CATG'y
Cuts at: 1132 1229 2084 2942 4487 1132
Size: 97 855 858 1545 2229
Fragments arranged by size:
2229 1545 858 855 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5584
PciI A'CATG_T
Cuts at: 4483 4483
Size: 5584
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2152 1108
Size: 1044 4540
PfoI T'CCnGG_A
Cuts at: 2859 2859
Size: 5584
PmeI GTTT'AAAC
Cuts at: 2888 2888
Size: 5584
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3653 3685 5166 5198 3653
Size: 32 1481 32 4039
Fragments arranged by size:
4039 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2149 2611 2149
Size: 462 5122
PshAI GACnn'nnGTC
Cuts at: 1613 2779 1613
Size: 1166 4418
PsiI TTA'TAA
Cuts at: 3154 3154
Size: 5584
PstI C_TGCA'G
Cuts at: 830 2333 2772 2936 830
Size: 1503 439 164 3478
Fragments arranged by size:
3478 1503 439 164
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5584
SalI G'TCGA_C
Cuts at: 1936 2926 1936
Size: 990 4594
SbfI CC_TGCA'GG
Cuts at: 2772 2936 2772
Size: 164 5420
ScaI AGT'ACT
Cuts at: 3832 5544 3832
Size: 1712 3872
SfcI C'TryA_G
Cuts at: 826 1045 2010 2329 2768 2932 4067 4748 4939
Size: 219 965 319 439 164 1135 681 191
Cuts at: 4939 826
Size: 1471
Fragments arranged by size:
1471 1135 965 681 439 319 219 191
164
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5584
SgrAI Cr'CCGG_yG
Cuts at: 1700 2699 1700
Size: 999 4585
SmaI CCC'GGG
Cuts at: 1872 2911 1872
Size: 1039 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3688 4589 4851 820
Size: 197 926 1745 901 262 1553
Fragments arranged by size:
1745 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3375 493
Size: 2882 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5584
SphI G_CATG'C
Cuts at: 1132 2942 1132
Size: 1810 3774
SspI AAT'ATT
Cuts at: 5 52 3508 5
Size: 47 3456 2081
Fragments arranged by size:
3456 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5584
StyI C'CwwG_G
Cuts at: 513 1065 2004 3023 513
Size: 552 939 1019 3074
Fragments arranged by size:
3074 1019 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2023 2249 3611 3628 3781 3966 4411
Size: 1255 443 226 1362 17 153 185 445
Cuts at: 4411 325
Size: 1498
Fragments arranged by size:
1498 1362 1255 445 443 226 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3830 5542 96
Size: 274 80 33 51 1443 1853 1712 138
Fragments arranged by size:
1853 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5544 5576 5544
Size: 32 5552
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5584
XbaI T'CTAG_A
Cuts at: 2920 2920
Size: 5584
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2735 2735
Size: 5584
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5584
XmaI C'CCGG_G
Cuts at: 1870 2909 1870
Size: 1039 4545
XmnI GAAnn'nnTTC
Cuts at: 2342 3713 2342
Size: 1371 4213
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3392 276
Size: 53 83 186 936 1858 2468
Fragments arranged by size:
2468 1858 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BstXI BstZ17I EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI