Restriction map for
pFN21AE3652
(Circular) (Six-base) MAPSORT of: pFN21AE3652.seq Check: 604 from: 1 to: 5803
pFN21AE3652 5803 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3613 278
Size: 53 83 186 936 2077 2468
Fragments arranged by size:
2468 2077 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3146 1937
Size: 1209 4594
Acc65I G'GTAC_C
Cuts at: 3124 3124
Size: 5803
AclI AA'CG_TT
Cuts at: 1563 3930 4303 1563
Size: 2367 373 3063
Fragments arranged by size:
3063 2367 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2320 2370 3866 5250 5518 1632
Size: 688 50 1496 1384 268 1917
Fragments arranged by size:
1917 1496 1384 688 268 50
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5606
AflIII A'CryG_T
Cuts at: 1203 2343 3019 4702 5679 1203
Size: 1140 676 1683 977 1327
Fragments arranged by size:
1683 1327 1140 977 676
AgeI A'CCGG_T
Cuts at: 4634 4634
Size: 5803
AhdI GACnn_n'nnGTC
Cuts at: 4532 4532
Size: 5803
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5803
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3108 1184
Size: 1924 3879
AlwNI CAG_nnn'CTG
Cuts at: 2068 2824 2927 3546 5118 2068
Size: 756 103 619 1572 2753
Fragments arranged by size:
2753 1572 756 619 103
ApaI G_GGCC'C
Cuts at: 2110 2133 2977 2110
Size: 23 844 4936
Fragments arranged by size:
4936 844 23
ApaLI G'TGCA_C
Cuts at: 3860 5016 3860
Size: 1156 4647
ApoI r'AATT_y
Cuts at: 1493 3112 3338 3490 5599 1493
Size: 1619 226 152 2109 1697
Fragments arranged by size:
2109 1697 1619 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4357 160
Size: 872 3325 1606
Fragments arranged by size:
3325 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5803
AvaI C'yCGr_G
Cuts at: 1870 1943 2079 2225 3128 1870
Size: 73 136 146 903 4545
Fragments arranged by size:
4545 903 146 136 73
BamHI G'GATC_C
Cuts at: 3133 3500 3133
Size: 367 5436
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3124 4579 618
Size: 325 217 627 1337 1455 1842
Fragments arranged by size:
1842 1455 1337 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2110 2133 2219 2226 2454 2695 2935 2977
Size: 719 23 86 7 228 241 240 42
Cuts at: 2977 3122 1391
Size: 145 4072
Fragments arranged by size:
4072 719 241 240 228 145 86 42
23 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2191 928
Size: 971 292 4540
Fragments arranged by size:
4540 971 292
BbvCI CC'TCA_GC
Cuts at: 2322 2322
Size: 5803
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3994 4028 1903
Size: 34 2057 34 3678
Fragments arranged by size:
3678 2057 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3695 4905 3695
Size: 1210 4593
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5803
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2318 4414 136
Size: 107 122 71 631 605 646 2096 1525
Fragments arranged by size:
2096 1525 646 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5798 5798
Size: 5803
BlpI GC'TnA_GC
Cuts at: 2620 3220 2620
Size: 600 5203
Bme1580I G_kGCm'C
Cuts at: 1790 2055 2110 2133 2429 2502 2977 3072 3864
Size: 265 55 23 296 73 475 95 792
Cuts at: 3864 5020 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 792 475 296 265 95 73
55 23
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5803
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2539 2670 2850
Size: 416 53 83 399 24 1122 131 180
Cuts at: 2850 3534 4492 442
Size: 684 958 1753
Fragments arranged by size:
1753 1122 958 684 416 399 180 131
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5803
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2260 2260
Size: 5803
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2090 2184 4463 1239
Size: 851 94 2279 2579
Fragments arranged by size:
2579 2279 851 94
Bpu10I CC'TnA_GC
Cuts at: 2322 2322
Size: 5803
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3928 4793 5091 3928
Size: 865 298 4640
Fragments arranged by size:
4640 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2733 4466 882
Size: 617 1234 1733 2219
Fragments arranged by size:
2219 1733 1234 617
BsaAI yAC'GTr
Cuts at: 493 1204 3594 493
Size: 711 2390 2702
Fragments arranged by size:
2702 2390 711
BsaBI GATnn'nnATC
Cuts at: 5797 5797
Size: 5803
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3610 3992 4627 275
Size: 53 83 186 936 2077 382 635 1451
Fragments arranged by size:
2077 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2761 4236 4634 4908 5055 5466 2761
Size: 1475 398 274 147 411 3098
Fragments arranged by size:
3098 1475 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2475 2505 1178
Size: 30 165 30 1072 30 4476
Fragments arranged by size:
4476 1072 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2249 2339 1186
Size: 1063 90 4650
Fragments arranged by size:
4650 1063 90
BseYI C'CCAG_C
Cuts at: 2263 2358 2391 2824 2829 2944 2949 2976 5006
Size: 95 33 433 5 115 5 27 2030
Cuts at: 5006 5502 2263
Size: 496 2564
Fragments arranged by size:
2564 2030 496 433 115 95 33 27
5 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1997 2525 2879 1163
Size: 573 261 528 354 4087
Fragments arranged by size:
4087 573 528 354 261
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3253 4014 5042 1540
Size: 275 186 1252 761 1028 2301
Fragments arranged by size:
2301 1252 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3864 3949 5020 3864
Size: 85 1071 4647
Fragments arranged by size:
4647 1071 85
BsmI GAATG_Cn'
Cuts at: 3314 3407 3314
Size: 93 5710
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2110 2133 2219 2226 2429 2454
Size: 399 265 55 23 86 7 203 25
Cuts at: 2454 2502 2695 2935 2977 3072 3122 3864 3949
Size: 48 193 240 42 95 50 742 85
Cuts at: 3949 5020 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 265 240 203 193
95 86 85 55 50 48 42 25
23 7
BspEI T'CCGG_A
Cuts at: 2761 5466 2761
Size: 2705 3098
BspHI T'CATG_A
Cuts at: 3692 5422 3692
Size: 1730 4073
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2624 3158 844
Size: 1780 534 3489
Fragments arranged by size:
3489 1780 534
BsrBI CCG'CTC
Cuts at: 1394 1823 3690 1394
Size: 429 1867 3507
Fragments arranged by size:
3507 1867 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4298 4472 65
Size: 1183 3050 174 1396
Fragments arranged by size:
3050 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2770 4447 4634 1700
Size: 110 960 1677 187 2869
Fragments arranged by size:
2869 1677 960 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5803
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5803
BssSI C'ACGA_G
Cuts at: 3863 4875 3863
Size: 1012 4791
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2020 794
Size: 1226 4577
BstBI TT'CG_AA
Cuts at: 4607 4607
Size: 5803
BstEII G'GTnAC_C
Cuts at: 1184 2140 1184
Size: 956 4847
BstXI CCAn_nnnn'nTGG
Cuts at: 2308 2509 2308
Size: 201 5602
BstYI r'GATC_y
Cuts at: 1972 3133 3500 3885 3902 5354 5440 5452 5798
Size: 1161 367 385 17 1452 86 12 346
Cuts at: 5798 1972
Size: 1977
Fragments arranged by size:
1977 1452 1161 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2631 2631
Size: 5803
BtgI C'CryG_G
Cuts at: 513 1065 2004 2136 2490 513
Size: 552 939 132 354 3826
Fragments arranged by size:
3826 939 552 354 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5064
Fragments arranged by size:
5064 683 56
BtsI GCAGTG_nn'
Cuts at: 3319 4113 4133 3319
Size: 794 20 4989
Fragments arranged by size:
4989 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5803
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5768
DraI TTT'AAA
Cuts at: 3107 3107
Size: 5803
DraIII CAC_nnn'GTG
Cuts at: 2048 2678 2048
Size: 630 5173
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2165 4810 809
Size: 507 849 2645 1802
Fragments arranged by size:
2645 1802 849 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2094 2351 2547
Size: 54 1425 50 275 18 264 257 196
Cuts at: 2547 3250 4139 5768 8
Size: 703 889 1629 43
Fragments arranged by size:
1629 1425 889 703 275 264 257 196
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3250 1537
Size: 275 1438 4090
Fragments arranged by size:
4090 1438 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2030 2226 2339 3733 1353
Size: 677 196 113 1394 3423
Fragments arranged by size:
3423 1394 677 196 113
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4390 4764 4910 387
Size: 1064 138 2801 374 146 1280
Fragments arranged by size:
2801 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2184 2320 2370 3866 4463 5250
Size: 393 458 94 136 50 1496 597 787
Cuts at: 5250 5518 1239
Size: 268 1524
Fragments arranged by size:
1524 1496 787 597 458 393 268 136
94 50
EcoO109I rG'GnC_Cy
Cuts at: 2106 2107 2129 2130 2158 2329 2486 2973 3012
Size: 1 22 1 28 171 157 487 39
Cuts at: 3012 2106
Size: 4897
Fragments arranged by size:
4897 487 171 157 39 28 22 1
1
EcoRI G'AATT_C
Cuts at: 3112 3490 3112
Size: 378 5425
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2273 2273
Size: 5803
FspI TGC'GCA
Cuts at: 4309 4309
Size: 5803
HaeII r_GCGC'y
Cuts at: 1678 4950 1678
Size: 3272 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2276 2308 4444 4476 4518
Size: 32 135 32 704 32 2136 32 42
Cuts at: 4518 4550 1373
Size: 32 2626
Fragments arranged by size:
2626 2136 704 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3147 3393 669
Size: 988 281 1209 246 3079
Fragments arranged by size:
3079 1209 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5803
HpaI GTT'AAC
Cuts at: 3393 3393
Size: 5803
KpnI G_GTAC'C
Cuts at: 3128 3128
Size: 5803
MfeI C'AATT_G
Cuts at: 3402 3402
Size: 5803
MluI A'CGCG_T
Cuts at: 5679 5679
Size: 5803
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4917 5101 1305
Size: 3612 184 2007
Fragments arranged by size:
3612 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2096 2549 10
Size: 54 1425 343 264 453 3264
Fragments arranged by size:
3264 1425 453 343 264 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3761 4120 4279 518
Size: 648 2595 359 159 2042
Fragments arranged by size:
2595 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2058 2138 2280 2297 2395 3219 3896 5044
Size: 452 80 142 17 98 824 677 1148
Cuts at: 5044 5289 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 824 677 452 245 142 98
80 17
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4312
Fragments arranged by size:
4312 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5803
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5803
NotI GC'GGCC_GC
Cuts at: 3250 3250
Size: 5803
NspI r_CATG'y
Cuts at: 1132 1229 2192 2347 3023 3161 4706 1132
Size: 97 963 155 676 138 1545 2229
Fragments arranged by size:
2229 1545 963 676 155 138 97
PasI CC'CwG_GG
Cuts at: 1348 2194 2927 2999 1348
Size: 846 733 72 4152
Fragments arranged by size:
4152 846 733 72
PciI A'CATG_T
Cuts at: 2343 3019 4702 2343
Size: 676 1683 3444
Fragments arranged by size:
3444 1683 676
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2048 2068 2678 2824 2927 1108
Size: 940 20 610 146 103 3984
Fragments arranged by size:
3984 940 610 146 103 20
PfoI T'CCnGG_A
Cuts at: 2817 2817
Size: 5803
PmeI GTTT'AAAC
Cuts at: 3107 3107
Size: 5803
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3872 3904 5385 5417 3872
Size: 32 1481 32 4258
Fragments arranged by size:
4258 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2158 2486 2158
Size: 328 5475
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5803
PsiI TTA'TAA
Cuts at: 3373 3373
Size: 5803
PspOMI G'GGCC_C
Cuts at: 2106 2129 2973 2106
Size: 23 844 4936
Fragments arranged by size:
4936 844 23
PstI C_TGCA'G
Cuts at: 830 2754 3155 830
Size: 1924 401 3478
Fragments arranged by size:
3478 1924 401
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5803
PvuII CAG'CTG
Cuts at: 2058 2280 2297 2395 2058
Size: 222 17 98 5466
Fragments arranged by size:
5466 222 98 17
SacII CC_GC'GG
Cuts at: 2139 2139
Size: 5803
SalI G'TCGA_C
Cuts at: 1936 3145 1936
Size: 1209 4594
SanDI GG'GwC_CC
Cuts at: 2158 2158
Size: 5803
SapI GCTCTTCn'nnn_
Cuts at: 2030 2030
Size: 5803
SbfI CC_TGCA'GG
Cuts at: 3155 3155
Size: 5803
ScaI AGT'ACT
Cuts at: 4051 5763 4051
Size: 1712 4091
SfcI C'TryA_G
Cuts at: 826 1045 2750 3151 4286 4967 5158 826
Size: 219 1705 401 1135 681 191 1471
Fragments arranged by size:
1705 1471 1135 681 401 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5803
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5803
SmaI CCC'GGG
Cuts at: 1872 2081 3130 1872
Size: 209 1049 4545
Fragments arranged by size:
4545 1049 209
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3907 4808 5070 820
Size: 197 926 1964 901 262 1553
Fragments arranged by size:
1964 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3594 493
Size: 3101 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5803
SphI G_CATG'C
Cuts at: 1132 3161 1132
Size: 2029 3774
SspI AAT'ATT
Cuts at: 5 52 3727 5
Size: 47 3675 2081
Fragments arranged by size:
3675 2081 47
StuI AGG'CCT
Cuts at: 1517 2463 2758 1517
Size: 946 295 4562
Fragments arranged by size:
4562 946 295
StyI C'CwwG_G
Cuts at: 513 1065 2004 2097 2124 2723 3242 513
Size: 552 939 93 27 599 519 3074
Fragments arranged by size:
3074 939 599 552 519 93 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2689 3830 3847 4000 4185 4630 325
Size: 1255 1109 1141 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1141 1109 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4049 5761 96
Size: 274 80 33 51 1443 2072 1712 138
Fragments arranged by size:
2072 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2475 2507 5763 5795 2475
Size: 32 3256 32 2483
Fragments arranged by size:
3256 2483 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5803
XbaI T'CTAG_A
Cuts at: 3139 3139
Size: 5803
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2644 2876 2644
Size: 232 5571
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5803
XmaI C'CCGG_G
Cuts at: 1870 2079 3128 1870
Size: 209 1049 4545
Fragments arranged by size:
4545 1049 209
XmnI GAAnn'nnTTC
Cuts at: 2088 3932 2088
Size: 1844 3959
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3611 276
Size: 53 83 186 936 2077 2468
Fragments arranged by size:
2468 2077 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacI SexAI SfiI SfoI SrfI
SwaI