Restriction map for
pFN21AE3651
(Circular) (Six-base) MAPSORT of: pFN21AE3651.seq Check: 279 from: 1 to: 7399
pFN21AE3651 7399 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5209 278
Size: 53 83 186 936 3673 2468
Fragments arranged by size:
3673 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4742 1937
Size: 2805 4594
Acc65I G'GTAC_C
Cuts at: 4720 4720
Size: 7399
AclI AA'CG_TT
Cuts at: 1563 2723 5526 5899 1563
Size: 1160 2803 373 3063
Fragments arranged by size:
3063 2803 1160 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2042 2345 3470 3807 4547 5462 6846 7114
Size: 410 303 1125 337 740 915 1384 268
Cuts at: 7114 1632
Size: 1917
Fragments arranged by size:
1917 1384 1125 915 740 410 337 303
268
AfeI AGC'GCT
Cuts at: 3305 3305
Size: 7399
AflII C'TTAA_G
Cuts at: 820 1017 2213 820
Size: 197 1196 6006
Fragments arranged by size:
6006 1196 197
AflIII A'CryG_T
Cuts at: 1203 2854 3999 6298 7275 1203
Size: 1651 1145 2299 977 1327
Fragments arranged by size:
2299 1651 1327 1145 977
AgeI A'CCGG_T
Cuts at: 6230 6230
Size: 7399
AhdI GACnn_n'nnGTC
Cuts at: 6128 6128
Size: 7399
AleI CACnn'nnGTG
Cuts at: 1166 2142 1166
Size: 976 6423
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7399
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4470 4502 4470
Size: 32 7367
AlwNI CAG_nnn'CTG
Cuts at: 2330 3820 5142 6714 2330
Size: 1490 1322 1572 3015
Fragments arranged by size:
3015 1572 1490 1322
ApaLI G'TGCA_C
Cuts at: 5456 6612 5456
Size: 1156 6243
ApoI r'AATT_y
Cuts at: 1493 2225 4660 4708 4934 5086 7195 1493
Size: 732 2435 48 226 152 2109 1697
Fragments arranged by size:
2435 2109 1697 732 226 152 48
AseI AT'TA_AT
Cuts at: 160 1032 5953 160
Size: 872 4921 1606
Fragments arranged by size:
4921 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7399
AvaI C'yCGr_G
Cuts at: 1870 1943 3972 4724 1870
Size: 73 2029 752 4545
Fragments arranged by size:
4545 2029 752 73
BamHI G'GATC_C
Cuts at: 4729 5096 4729
Size: 367 7032
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3408 4720 6175 618
Size: 325 217 627 1621 1312 1455 1842
Fragments arranged by size:
1842 1621 1455 1312 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4488 4718 1391
Size: 3097 230 4072
Fragments arranged by size:
4072 3097 230
BbeI G_GCGC'C
Cuts at: 3412 3412
Size: 7399
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2997 928
Size: 971 1098 5330
Fragments arranged by size:
5330 1098 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3878 3912 5590 5624 1903
Size: 34 50 34 1857 34 1678 34 3678
Fragments arranged by size:
3678 1857 1678 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5291 6501 5291
Size: 1210 6189
BclI T'GATC_A
Cuts at: 1548 3864 1548
Size: 2316 5083
BfrBI ATG'CAT
Cuts at: 2429 2429
Size: 7399
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4307 6010 136
Size: 107 122 71 631 605 2635 1703 1525
Fragments arranged by size:
2635 1703 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3612 7394 3612
Size: 3782 3617
BlpI GC'TnA_GC
Cuts at: 2323 4816 2323
Size: 2493 4906
Bme1580I G_kGCm'C
Cuts at: 1790 2546 5460 6616 1790
Size: 756 2914 1156 2573
Fragments arranged by size:
2914 2573 1156 756
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7399
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3256 5130 6088
Size: 416 53 83 399 24 1839 1874 958
Cuts at: 6088 442
Size: 1753
Fragments arranged by size:
1874 1839 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7399
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3766 4527 3766
Size: 761 6638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2547 3458 3548 4085 4394 4472 6059 1239
Size: 1308 911 90 537 309 78 1587 2579
Fragments arranged by size:
2579 1587 1308 911 537 309 90 78
Bpu10I CC'TnA_GC
Cuts at: 2317 3246 2317
Size: 929 6470
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3461 4199 4335 4359 4417 4550 5524 6389 6687
Size: 738 136 24 58 133 974 865 298
Cuts at: 6687 3461
Size: 4173
Fragments arranged by size:
4173 974 865 738 298 136 133 58
24
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2835 6062 882
Size: 617 1336 3227 2219
Fragments arranged by size:
3227 2219 1336 617
BsaAI yAC'GTr
Cuts at: 493 1204 2855 5190 493
Size: 711 1651 2335 2702
Fragments arranged by size:
2702 2335 1651 711
BsaBI GATnn'nnATC
Cuts at: 7393 7393
Size: 7399
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3409 5206 5588 6223
Size: 53 83 186 936 1876 1797 382 635
Cuts at: 6223 275
Size: 1451
Fragments arranged by size:
1876 1797 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3291 5832 6230 6504 6651 7062 3291
Size: 2541 398 274 147 411 3628
Fragments arranged by size:
3628 2541 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3356 3386 4470 4500 1178
Size: 30 165 30 1953 30 1084 30 4077
Fragments arranged by size:
4077 1953 1084 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3169 4562 1186
Size: 1983 1393 4023
Fragments arranged by size:
4023 1983 1393
BseYI C'CCAG_C
Cuts at: 3621 6602 7098 3621
Size: 2981 496 3922
Fragments arranged by size:
3922 2981 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3980 4079 1163
Size: 573 2244 99 4483
Fragments arranged by size:
4483 2244 573 99
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4849 5610 6638 1540
Size: 275 186 2848 761 1028 2301
Fragments arranged by size:
2848 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3074 3471 4254 4488 4525 5460 5545 6616 3074
Size: 397 783 234 37 935 85 1071 3857
Fragments arranged by size:
3857 1071 935 783 397 234 85 37
BsmI GAATG_Cn'
Cuts at: 2683 4391 4910 5003 2683
Size: 1708 519 93 5079
Fragments arranged by size:
5079 1708 519 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3491 3491
Size: 7399
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2546 3074 3471 4254 4488 4525 4718
Size: 399 756 528 397 783 234 37 193
Cuts at: 4718 5460 5545 6616 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 783 756 742 528 399 397
234 193 85 37
BspEI T'CCGG_A
Cuts at: 7062 7062
Size: 7399
BspHI T'CATG_A
Cuts at: 3141 5288 7018 3141
Size: 2147 1730 3522
Fragments arranged by size:
3522 2147 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4754 844
Size: 3910 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3745 5286 1394
Size: 429 1922 1541 3507
Fragments arranged by size:
3507 1922 1541 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2688 2700 5894 6068 65
Size: 1183 1440 12 3194 174 1396
Fragments arranged by size:
3194 1440 1396 1183 174 12
BsrFI r'CCGG_y
Cuts at: 1700 1810 2844 6043 6230 1700
Size: 110 1034 3199 187 2869
Fragments arranged by size:
3199 2869 1034 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7399
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7399
BssSI C'ACGA_G
Cuts at: 3875 5459 6471 3875
Size: 1584 1012 4803
Fragments arranged by size:
4803 1584 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2687 3946 794
Size: 1893 1259 4247
Fragments arranged by size:
4247 1893 1259
BstBI TT'CG_AA
Cuts at: 6203 6203
Size: 7399
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7399
BstXI CCAn_nnnn'nTGG
Cuts at: 2149 3526 2149
Size: 1377 6022
BstYI r'GATC_y
Cuts at: 1972 2504 3345 3612 3747 4021 4729 5096 5481
Size: 532 841 267 135 274 708 367 385
Cuts at: 5481 5498 6950 7036 7048 7394 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 841 708 532 385 367 346
274 267 135 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2064 2790 4683 2064
Size: 726 1893 4780
Fragments arranged by size:
4780 1893 726
BtgI C'CryG_G
Cuts at: 513 1065 3229 3251 4146 513
Size: 552 2164 22 895 3766
Fragments arranged by size:
3766 2164 895 552 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6660
Fragments arranged by size:
6660 683 56
BtsI GCAGTG_nn'
Cuts at: 2289 3097 4915 5709 5729 2289
Size: 808 1818 794 20 3959
Fragments arranged by size:
3959 1818 808 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7399
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7364
DraI TTT'AAA
Cuts at: 4703 4703
Size: 7399
DraIII CAC_nnn'GTG
Cuts at: 2855 3880 2855
Size: 1025 6374
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6406 809
Size: 507 5090 1802
Fragments arranged by size:
5090 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2846 3537 4846
Size: 54 1425 50 275 18 1016 691 1309
Cuts at: 4846 5735 7364 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1309 1016 889 691 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4846 1537
Size: 275 3034 4090
Fragments arranged by size:
4090 3034 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3783 4539 5329 1353
Size: 2430 756 790 3423
Fragments arranged by size:
3423 2430 790 756
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2663 5986 6360 6506 387
Size: 1064 138 1074 3323 374 146 1280
Fragments arranged by size:
3323 1280 1074 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4486 4486
Size: 7399
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2345 2547 3458 3470 3548 3807
Size: 393 410 303 202 911 12 78 259
Cuts at: 3807 4085 4394 4472 4547 5462 6059 6846 7114
Size: 278 309 78 75 915 597 787 268
Cuts at: 7114 1239
Size: 1524
Fragments arranged by size:
1524 915 911 787 597 410 393 309
303 278 268 259 202 78 78 75
12
EcoNI CCTnn'n_nnAGG
Cuts at: 4565 4565
Size: 7399
EcoO109I rG'GnC_Cy
Cuts at: 2026 3280 3921 2026
Size: 1254 641 5504
Fragments arranged by size:
5504 1254 641
EcoRI G'AATT_C
Cuts at: 4708 5086 4708
Size: 378 7021
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2228 3818 2228
Size: 1590 5809
FspI TGC'GCA
Cuts at: 4517 5905 4517
Size: 1388 6011
HaeII r_GCGC'y
Cuts at: 1678 3307 3412 6546 1678
Size: 1629 105 3134 2531
Fragments arranged by size:
3134 2531 1629 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2026 2058 2489 2521 2603
Size: 32 135 32 454 32 431 32 82
Cuts at: 2603 2635 4333 4365 6040 6072 6114 6146 1373
Size: 32 1698 32 1675 32 42 32 2626
Fragments arranged by size:
2626 1698 1675 454 431 135 82 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4743 4989 669
Size: 988 281 2805 246 3079
Fragments arranged by size:
3079 2805 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7399
HpaI GTT'AAC
Cuts at: 4989 4989
Size: 7399
KasI G'GCGC_C
Cuts at: 3408 3408
Size: 7399
KpnI G_GTAC'C
Cuts at: 4724 4724
Size: 7399
MfeI C'AATT_G
Cuts at: 2237 4998 2237
Size: 2761 4638
MluI A'CGCG_T
Cuts at: 7275 7275
Size: 7399
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4209 6513 6697 1305
Size: 2904 2304 184 2007
Fragments arranged by size:
2904 2304 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3539 10
Size: 54 1425 343 1707 3870
Fragments arranged by size:
3870 1707 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2142 2424 3065 3354 4151 4479 5357
Size: 648 976 282 641 289 797 328 878
Cuts at: 5357 5716 5875 518
Size: 359 159 2042
Fragments arranged by size:
2042 976 878 797 648 641 359 328
289 282 159
MspA1I CmG'CkG
Cuts at: 1606 3253 3427 3994 4063 4815 5492 6640 6885
Size: 1647 174 567 69 752 677 1148 245
Cuts at: 6885 1606
Size: 2120
Fragments arranged by size:
2120 1647 1148 752 677 567 245 174
69
NarI GG'CG_CC
Cuts at: 3409 3409
Size: 7399
NcoI C'CATG_G
Cuts at: 513 1065 4146 513
Size: 552 3081 3766
Fragments arranged by size:
3766 3081 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7399
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7399
NotI GC'GGCC_GC
Cuts at: 4846 4846
Size: 7399
NsiI A_TGCA'T
Cuts at: 2431 2431
Size: 7399
NspI r_CATG'y
Cuts at: 1132 1229 3064 3106 3506 3944 4109 4757 6302
Size: 97 1835 42 400 438 165 648 1545
Cuts at: 6302 1132
Size: 2229
Fragments arranged by size:
2229 1835 1545 648 438 400 165 97
42
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7399
PciI A'CATG_T
Cuts at: 6298 6298
Size: 7399
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7399
PmeI GTTT'AAAC
Cuts at: 4703 4703
Size: 7399
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3584 3616 4470 4502 5468 5500 6981 7013 3584
Size: 32 854 32 966 32 1481 32 3970
Fragments arranged by size:
3970 1481 966 854 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2026 3921 2026
Size: 1895 5504
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7399
PsiI TTA'TAA
Cuts at: 3396 4969 3396
Size: 1573 5826
PstI C_TGCA'G
Cuts at: 830 3586 4751 830
Size: 2756 1165 3478
Fragments arranged by size:
3478 2756 1165
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7399
PvuII CAG'CTG
Cuts at: 3427 3994 4063 3427
Size: 567 69 6763
Fragments arranged by size:
6763 567 69
SacI G_AGCT'C
Cuts at: 4488 4488
Size: 7399
SacII CC_GC'GG
Cuts at: 3254 3254
Size: 7399
SalI G'TCGA_C
Cuts at: 1936 4741 1936
Size: 2805 4594
SbfI CC_TGCA'GG
Cuts at: 4751 4751
Size: 7399
ScaI AGT'ACT
Cuts at: 5647 7359 5647
Size: 1712 5687
SfcI C'TryA_G
Cuts at: 826 1045 2173 2912 3482 3582 4403 4693 4747
Size: 219 1128 739 570 100 821 290 54
Cuts at: 4747 5882 6563 6754 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1128 821 739 681 570 290
219 191 100 54
SfoI GGC'GCC
Cuts at: 3410 3410
Size: 7399
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7399
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7399
SmaI CCC'GGG
Cuts at: 1872 4726 1872
Size: 2854 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2213 3476 4214 4350 4374 4396
Size: 197 926 270 1263 738 136 24 22
Cuts at: 4396 4529 5503 6404 6666 820
Size: 133 974 901 262 1553
Fragments arranged by size:
1553 1263 974 926 901 738 270 262
197 136 133 24 22
SnaBI TAC'GTA
Cuts at: 493 5190 493
Size: 4697 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7399
SphI G_CATG'C
Cuts at: 1132 3106 3506 3944 4757 1132
Size: 1974 400 438 813 3774
Fragments arranged by size:
3774 1974 813 438 400
SspI AAT'ATT
Cuts at: 5 52 2312 2461 5323 5
Size: 47 2260 149 2862 2081
Fragments arranged by size:
2862 2260 2081 149 47
StuI AGG'CCT
Cuts at: 1517 3244 4394 1517
Size: 1727 1150 4522
Fragments arranged by size:
4522 1727 1150
StyI C'CwwG_G
Cuts at: 513 1065 4146 4838 513
Size: 552 3081 692 3074
Fragments arranged by size:
3081 3074 692 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3739 5426 5443 5596 5781 6226 325
Size: 1255 2159 1687 17 153 185 445 1498
Fragments arranged by size:
2159 1687 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2262 3822 3984
Size: 274 80 33 51 1443 285 1560 162
Cuts at: 3984 4068 4189 5645 7357 96
Size: 84 121 1456 1712 138
Fragments arranged by size:
1712 1560 1456 1443 285 274 162 138
121 84 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2841 2873 3330 3362 3950 3982 7359 7391 2841
Size: 32 457 32 588 32 3377 32 2849
Fragments arranged by size:
3377 2849 588 457 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7399
XbaI T'CTAG_A
Cuts at: 4735 4735
Size: 7399
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2149 2536 3547 2149
Size: 387 1011 6001
Fragments arranged by size:
6001 1011 387
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7399
XmaI C'CCGG_G
Cuts at: 1870 4724 1870
Size: 2854 4545
XmnI GAAnn'nnTTC
Cuts at: 3599 5528 3599
Size: 1929 5470
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5207 276
Size: 53 83 186 936 3673 2468
Fragments arranged by size:
3673 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbvCI BsiWI BstZ17I
EcoRV FseI FspAI NaeI NgoMIV NruI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SanDI SapI SexAI
SfiI SrfI SwaI