Restriction map for
pFN21AE3644
(Circular) (Six-base) MAPSORT of: pFN21AE3644.seq Check: 4776 from: 1 to: 6307
pFN21AE3644 6307 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4117 278
Size: 53 83 186 936 2581 2468
Fragments arranged by size:
2581 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3650 1937
Size: 1713 4594
Acc65I G'GTAC_C
Cuts at: 3628 3628
Size: 6307
AclI AA'CG_TT
Cuts at: 1563 3036 4434 4807 1563
Size: 1473 1398 373 3063
Fragments arranged by size:
3063 1473 1398 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2283 2716 3222 3383 3573 4370 5754 6022
Size: 651 433 506 161 190 797 1384 268
Cuts at: 6022 1632
Size: 1917
Fragments arranged by size:
1917 1384 797 651 506 433 268 190
161
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6110
AflIII A'CryG_T
Cuts at: 1203 5206 6183 1203
Size: 4003 977 1327
Fragments arranged by size:
4003 1327 977
AgeI A'CCGG_T
Cuts at: 5138 5138
Size: 6307
AhdI GACnn_n'nnGTC
Cuts at: 5036 5036
Size: 6307
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6307
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6307
AlwNI CAG_nnn'CTG
Cuts at: 2132 4050 5622 2132
Size: 1918 1572 2817
Fragments arranged by size:
2817 1918 1572
ApaLI G'TGCA_C
Cuts at: 4364 5520 4364
Size: 1156 5151
ApoI r'AATT_y
Cuts at: 1493 2136 2276 2437 2754 2794 2942 2968 3446
Size: 643 140 161 317 40 148 26 478
Cuts at: 3446 3616 3842 3994 6103 1493
Size: 170 226 152 2109 1697
Fragments arranged by size:
2109 1697 643 478 317 226 170 161
152 148 140 40 26
AseI AT'TA_AT
Cuts at: 160 1032 4861 160
Size: 872 3829 1606
Fragments arranged by size:
3829 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6307
AvaI C'yCGr_G
Cuts at: 1870 1943 3632 1870
Size: 73 1689 4545
Fragments arranged by size:
4545 1689 73
BamHI G'GATC_C
Cuts at: 3637 4004 3637
Size: 367 5940
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3115 3628 5083 618
Size: 325 217 627 1328 513 1455 1842
Fragments arranged by size:
1842 1455 1328 627 513 325 217
BanII G_rGCy'C
Cuts at: 1391 2779 3626 1391
Size: 1388 847 4072
Fragments arranged by size:
4072 1388 847
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5336
BbvCI CC'TCA_GC
Cuts at: 2054 2054
Size: 6307
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4498 4532 1903
Size: 34 2561 34 3678
Fragments arranged by size:
3678 2561 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3211 4199 5409 3211
Size: 988 1210 4109
Fragments arranged by size:
4109 1210 988
BclI T'GATC_A
Cuts at: 1548 2068 2652 1548
Size: 520 584 5203
Fragments arranged by size:
5203 584 520
BfrBI ATG'CAT
Cuts at: 2540 2540
Size: 6307
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4918 136
Size: 107 122 71 631 605 3246 1525
Fragments arranged by size:
3246 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6302 6302
Size: 6307
BlpI GC'TnA_GC
Cuts at: 2419 3468 3724 2419
Size: 1049 256 5002
Fragments arranged by size:
5002 1049 256
Bme1580I G_kGCm'C
Cuts at: 1790 4368 5524 1790
Size: 2578 1156 2573
Fragments arranged by size:
2578 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6307
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4038 4996 442
Size: 416 53 83 399 24 2621 958 1753
Fragments arranged by size:
2621 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6307
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2763 4967 1239
Size: 1524 2204 2579
Fragments arranged by size:
2579 2204 1524
Bpu10I CC'TnA_GC
Cuts at: 2054 3196 2054
Size: 1142 5165
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2012 3495 4432 5297 5595 2012
Size: 1483 937 865 298 2724
Fragments arranged by size:
2724 1483 937 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4970 882
Size: 617 3471 2219
Fragments arranged by size:
3471 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4098 493
Size: 711 2894 2702
Fragments arranged by size:
2894 2702 711
BsaBI GATnn'nnATC
Cuts at: 2190 2607 2651 3190 6301 2190
Size: 417 44 539 3111 2196
Fragments arranged by size:
3111 2196 539 417 44
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4114 4496 5131 275
Size: 53 83 186 936 2581 382 635 1451
Fragments arranged by size:
2581 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4740 5138 5412 5559 5970 4740
Size: 398 274 147 411 5077
Fragments arranged by size:
5077 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2024 2054 1178
Size: 30 165 30 621 30 5431
Fragments arranged by size:
5431 621 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6307
BseYI C'CCAG_C
Cuts at: 2014 5510 6006 2014
Size: 3496 496 2315
Fragments arranged by size:
3496 2315 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5734
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2047 3757 4518 5546 1540
Size: 275 186 46 1710 761 1028 2301
Fragments arranged by size:
2301 1710 1028 761 275 186 46
BsiHKAI G_wGCw'C
Cuts at: 4368 4453 5524 4368
Size: 85 1071 5151
Fragments arranged by size:
5151 1071 85
BsmI GAATG_Cn'
Cuts at: 3488 3818 3911 3488
Size: 330 93 5884
Fragments arranged by size:
5884 330 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2779 3626 4368 4453 5524 1391
Size: 399 989 847 742 85 1071 2174
Fragments arranged by size:
2174 1071 989 847 742 399 85
BspEI T'CCGG_A
Cuts at: 5970 5970
Size: 6307
BspHI T'CATG_A
Cuts at: 4196 5926 4196
Size: 1730 4577
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3662 844
Size: 2818 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4194 1394
Size: 429 2371 3507
Fragments arranged by size:
3507 2371 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3480 4802 4976 65
Size: 1183 2232 1322 174 1396
Fragments arranged by size:
2232 1396 1322 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2046 4951 5138 1700
Size: 110 236 2905 187 2869
Fragments arranged by size:
2905 2869 236 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6307
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6307
BssSI C'ACGA_G
Cuts at: 2928 4367 5379 2928
Size: 1439 1012 3856
Fragments arranged by size:
3856 1439 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6307
BstBI TT'CG_AA
Cuts at: 5111 5111
Size: 6307
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6307
BstXI CCAn_nnnn'nTGG
Cuts at: 2916 2916
Size: 6307
BstYI r'GATC_y
Cuts at: 1972 2548 2844 3637 4004 4389 4406 5858 5944
Size: 576 296 793 367 385 17 1452 86
Cuts at: 5944 5956 6302 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 793 576 385 367 346 296
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2271 2642 513
Size: 552 939 267 371 4178
Fragments arranged by size:
4178 939 552 371 267
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2313 487
Size: 683 56 1087 4481
Fragments arranged by size:
4481 1087 683 56
BtsI GCAGTG_nn'
Cuts at: 3823 4617 4637 3823
Size: 794 20 5493
Fragments arranged by size:
5493 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6307
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3431 3466 529
Size: 35 2867 35 3370
Fragments arranged by size:
3370 2867 35 35
DraI TTT'AAA
Cuts at: 3611 3611
Size: 6307
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5314 809
Size: 507 3998 1802
Fragments arranged by size:
3998 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2949 3109 3754
Size: 54 1425 50 275 18 1119 160 645
Cuts at: 3754 4643 6272 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1119 889 645 275 160 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3754 1537
Size: 275 1942 4090
Fragments arranged by size:
4090 1942 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2316 2784 4237 1353
Size: 963 468 1453 3423
Fragments arranged by size:
3423 1453 963 468
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4894 5268 5414 387
Size: 1064 138 3305 374 146 1280
Fragments arranged by size:
3305 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2283 2716 2763 3222 3383 3573 4370
Size: 393 651 433 47 459 161 190 797
Cuts at: 4370 4967 5754 6022 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 797 787 651 597 459 433 393
268 190 161 47
EcoNI CCTnn'n_nnAGG
Cuts at: 2979 2979
Size: 6307
EcoRI G'AATT_C
Cuts at: 2276 2437 2754 3616 3994 2276
Size: 161 317 862 378 4589
Fragments arranged by size:
4589 862 378 317 161
EcoRV GAT'ATC
Cuts at: 3329 3329
Size: 6307
FspI TGC'GCA
Cuts at: 4813 4813
Size: 6307
HaeII r_GCGC'y
Cuts at: 1678 5454 1678
Size: 3776 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2911 2943 4948 4980 5022
Size: 32 135 32 1339 32 2005 32 42
Cuts at: 5022 5054 1373
Size: 32 2626
Fragments arranged by size:
2626 2005 1339 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3651 3897 669
Size: 988 281 1713 246 3079
Fragments arranged by size:
3079 1713 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6307
HpaI GTT'AAC
Cuts at: 3897 3897
Size: 6307
KpnI G_GTAC'C
Cuts at: 3632 3632
Size: 6307
MfeI C'AATT_G
Cuts at: 3906 3906
Size: 6307
MluI A'CGCG_T
Cuts at: 6183 6183
Size: 6307
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5421 5605 1305
Size: 4116 184 2007
Fragments arranged by size:
4116 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2951 3111 10
Size: 54 1425 343 1119 160 3206
Fragments arranged by size:
3206 1425 1119 343 160 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4265 4624 4783 518
Size: 648 3099 359 159 2042
Fragments arranged by size:
3099 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2014 2053 2202 3071 3723 4400 5548 5793
Size: 408 39 149 869 652 677 1148 245
Cuts at: 5793 1606
Size: 2120
Fragments arranged by size:
2120 1148 869 677 652 408 245 149
39
NcoI C'CATG_G
Cuts at: 513 1065 2004 2271 2642 513
Size: 552 939 267 371 4178
Fragments arranged by size:
4178 939 552 371 267
NdeI CA'TA_TG
Cuts at: 387 2470 2613 387
Size: 2083 143 4081
Fragments arranged by size:
4081 2083 143
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6307
NotI GC'GGCC_GC
Cuts at: 3754 3754
Size: 6307
NsiI A_TGCA'T
Cuts at: 2542 2542
Size: 6307
NspI r_CATG'y
Cuts at: 1132 1229 3580 3665 5210 1132
Size: 97 2351 85 1545 2229
Fragments arranged by size:
2351 2229 1545 97 85
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6307
PciI A'CATG_T
Cuts at: 5206 5206
Size: 6307
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2165 1108
Size: 1057 5250
PfoI T'CCnGG_A
Cuts at: 3331 3331
Size: 6307
PmeI GTTT'AAAC
Cuts at: 3611 3611
Size: 6307
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4376 4408 5889 5921 4376
Size: 32 1481 32 4762
Fragments arranged by size:
4762 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2631 1613
Size: 1018 5289
PsiI TTA'TAA
Cuts at: 2501 3877 2501
Size: 1376 4931
PstI C_TGCA'G
Cuts at: 830 3102 3158 3659 830
Size: 2272 56 501 3478
Fragments arranged by size:
3478 2272 501 56
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6307
PvuII CAG'CTG
Cuts at: 2014 2053 2014
Size: 39 6268
SalI G'TCGA_C
Cuts at: 1936 3649 1936
Size: 1713 4594
SbfI CC_TGCA'GG
Cuts at: 3659 3659
Size: 6307
ScaI AGT'ACT
Cuts at: 4555 6267 4555
Size: 1712 4595
SexAI A'CCwGG_T
Cuts at: 2158 2158
Size: 6307
SfcI C'TryA_G
Cuts at: 826 1045 2365 2461 3098 3154 3655 4790 5471
Size: 219 1320 96 637 56 501 1135 681
Cuts at: 5471 5662 826
Size: 191 1471
Fragments arranged by size:
1471 1320 1135 681 637 501 219 191
96 56
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6307
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6307
SmaI CCC'GGG
Cuts at: 1872 3634 1872
Size: 1762 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2027 3510 4411 5312 5574 820
Size: 197 926 84 1483 901 901 262 1553
Fragments arranged by size:
1553 1483 926 901 901 262 197 84
SnaBI TAC'GTA
Cuts at: 493 4098 493
Size: 3605 2702
SpeI A'CTAG_T
Cuts at: 152 2476 152
Size: 2324 3983
SphI G_CATG'C
Cuts at: 1132 3665 1132
Size: 2533 3774
SspI AAT'ATT
Cuts at: 5 52 2666 4231 5
Size: 47 2614 1565 2081
Fragments arranged by size:
2614 2081 1565 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6307
StyI C'CwwG_G
Cuts at: 513 1065 2004 2271 2642 3746 513
Size: 552 939 267 371 1104 3074
Fragments arranged by size:
3074 1104 939 552 371 267
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4334 4351 4504 4689 5134 325
Size: 1255 2754 17 153 185 445 1498
Fragments arranged by size:
2754 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3279 4553 6265
Size: 274 80 33 51 1443 1302 1274 1712
Cuts at: 6265 96
Size: 138
Fragments arranged by size:
1712 1443 1302 1274 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2301 2333 6267 6299 2301
Size: 32 3934 32 2309
Fragments arranged by size:
3934 2309 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6307
XbaI T'CTAG_A
Cuts at: 2341 3643 2341
Size: 1302 5005
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6307
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6307
XmaI C'CCGG_G
Cuts at: 1870 3632 1870
Size: 1762 4545
XmnI GAAnn'nnTTC
Cuts at: 2337 2589 4436 2337
Size: 252 1847 4208
Fragments arranged by size:
4208 1847 252
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4115 276
Size: 53 83 186 936 2581 2468
Fragments arranged by size:
2581 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI