Restriction map for
pFN21AE3643
(Circular) (Six-base) MAPSORT of: pFN21AE3643.seq Check: 8382 from: 1 to: 5296
pFN21AE3643 5296 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3106 278
Size: 53 83 186 936 1570 2468
Fragments arranged by size:
2468 1570 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2639 1937
Size: 702 4594
Acc65I G'GTAC_C
Cuts at: 2184 2617 2184
Size: 433 4863
AclI AA'CG_TT
Cuts at: 1563 3423 3796 1563
Size: 1860 373 3063
Fragments arranged by size:
3063 1860 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2045 3359 4743 5011 1632
Size: 413 1314 1384 268 1917
Fragments arranged by size:
1917 1384 1314 413 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5099
AflIII A'CryG_T
Cuts at: 1203 2040 2072 4195 5172 1203
Size: 837 32 2123 977 1327
Fragments arranged by size:
2123 1327 977 837 32
AgeI A'CCGG_T
Cuts at: 4127 4127
Size: 5296
AhdI GACnn_n'nnGTC
Cuts at: 4025 4025
Size: 5296
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5296
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5296
AlwNI CAG_nnn'CTG
Cuts at: 2510 3039 4611 2510
Size: 529 1572 3195
Fragments arranged by size:
3195 1572 529
ApaLI G'TGCA_C
Cuts at: 3353 4509 3353
Size: 1156 4140
ApoI r'AATT_y
Cuts at: 1493 2396 2579 2605 2831 2983 5092 1493
Size: 903 183 26 226 152 2109 1697
Fragments arranged by size:
2109 1697 903 226 183 152 26
AseI AT'TA_AT
Cuts at: 160 1032 3850 160
Size: 872 2818 1606
Fragments arranged by size:
2818 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5296
AvaI C'yCGr_G
Cuts at: 1870 1943 2621 1870
Size: 73 678 4545
Fragments arranged by size:
4545 678 73
BamHI G'GATC_C
Cuts at: 2626 2993 2626
Size: 367 4929
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2184 2617 4072 618
Size: 325 217 627 397 433 1455 1842
Fragments arranged by size:
1842 1455 627 433 397 325 217
BanII G_rGCy'C
Cuts at: 1391 2113 2615 1391
Size: 722 502 4072
Fragments arranged by size:
4072 722 502
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4325
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3487 3521 1903
Size: 34 50 34 1466 34 3678
Fragments arranged by size:
3678 1466 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3188 4398 3188
Size: 1210 4086
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5296
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3907 136
Size: 107 122 71 631 605 2235 1525
Fragments arranged by size:
2235 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5291 5291
Size: 5296
BlpI GC'TnA_GC
Cuts at: 2713 2713
Size: 5296
Bme1580I G_kGCm'C
Cuts at: 1790 3357 4513 1790
Size: 1567 1156 2573
Fragments arranged by size:
2573 1567 1156
BmgBI CAC'GTC
Cuts at: 1318 2021 1318
Size: 703 4593
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3027 3985 442
Size: 416 53 83 399 24 1610 958 1753
Fragments arranged by size:
1753 1610 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5296
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2503 3956 1239
Size: 1264 1453 2579
Fragments arranged by size:
2579 1453 1264
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2408 3421 4286 4584 2408
Size: 1013 865 298 3120
Fragments arranged by size:
3120 1013 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2293 3959 882
Size: 617 794 1666 2219
Fragments arranged by size:
2219 1666 794 617
BsaAI yAC'GTr
Cuts at: 493 1204 3087 493
Size: 711 1883 2702
Fragments arranged by size:
2702 1883 711
BsaBI GATnn'nnATC
Cuts at: 5290 5290
Size: 5296
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3103 3485 4120 275
Size: 53 83 186 936 1570 382 635 1451
Fragments arranged by size:
1570 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3729 4127 4401 4548 4959 3729
Size: 398 274 147 411 4066
Fragments arranged by size:
4066 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5071
Fragments arranged by size:
5071 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2190 1186
Size: 1004 4292
BseYI C'CCAG_C
Cuts at: 4499 4995 4499
Size: 496 4800
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2021 1163
Size: 573 285 4438
Fragments arranged by size:
4438 573 285
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2746 3507 4535 1540
Size: 275 186 745 761 1028 2301
Fragments arranged by size:
2301 1028 761 745 275 186
BsiHKAI G_wGCw'C
Cuts at: 2113 3357 3442 4513 2113
Size: 1244 85 1071 2896
Fragments arranged by size:
2896 1244 1071 85
BsmI GAATG_Cn'
Cuts at: 2434 2807 2900 2434
Size: 373 93 4830
Fragments arranged by size:
4830 373 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2113 2615 3357 3442 4513 1391
Size: 399 323 502 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 502 399 323 85
BspEI T'CCGG_A
Cuts at: 4959 4959
Size: 5296
BspHI T'CATG_A
Cuts at: 3185 4915 3185
Size: 1730 3566
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2112 2651 844
Size: 1268 539 3489
Fragments arranged by size:
3489 1268 539
BsrBI CCG'CTC
Cuts at: 1394 1823 2374 3183 1394
Size: 429 551 809 3507
Fragments arranged by size:
3507 809 551 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3791 3965 65
Size: 1183 2543 174 1396
Fragments arranged by size:
2543 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3940 4127 1700
Size: 110 2130 187 2869
Fragments arranged by size:
2869 2130 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5296
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5296
BssSI C'ACGA_G
Cuts at: 3356 4368 3356
Size: 1012 4284
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5296
BstBI TT'CG_AA
Cuts at: 4100 4100
Size: 5296
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5296
BstYI r'GATC_y
Cuts at: 1972 2205 2626 2993 3378 3395 4847 4933 4945
Size: 233 421 367 385 17 1452 86 12
Cuts at: 4945 5291 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 421 385 367 346 233 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4744
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2299 487
Size: 683 56 1073 3484
Fragments arranged by size:
3484 1073 683 56
BtsI GCAGTG_nn'
Cuts at: 2812 3606 3626 2812
Size: 794 20 4482
Fragments arranged by size:
4482 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5296
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5261
DraI TTT'AAA
Cuts at: 2401 2600 2401
Size: 199 5097
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4303 809
Size: 507 2987 1802
Fragments arranged by size:
2987 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2743 3632 5261
Size: 54 1425 50 275 18 913 889 1629
Cuts at: 5261 8
Size: 43
Fragments arranged by size:
1629 1425 913 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2743 1537
Size: 275 931 4090
Fragments arranged by size:
4090 931 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2021 2478 3226 1353
Size: 668 457 748 3423
Fragments arranged by size:
3423 748 668 457
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3883 4257 4403 387
Size: 1064 138 2294 374 146 1280
Fragments arranged by size:
2294 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2111 2111
Size: 5296
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2503 3359 3956 4743 5011 1239
Size: 393 413 458 856 597 787 268 1524
Fragments arranged by size:
1524 856 787 597 458 413 393 268
EcoRI G'AATT_C
Cuts at: 2605 2983 2605
Size: 378 4918
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2434 2507 2434
Size: 73 5223
FspI TGC'GCA
Cuts at: 3802 3802
Size: 5296
HaeII r_GCGC'y
Cuts at: 1678 4443 1678
Size: 2765 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2292 2324 2375 2407 3937
Size: 32 135 32 720 32 51 32 1530
Cuts at: 3937 3969 4011 4043 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1530 720 135 51 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2328 2640 2886 669
Size: 988 281 390 312 246 3079
Fragments arranged by size:
3079 988 390 312 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5296
HpaI GTT'AAC
Cuts at: 2886 2886
Size: 5296
KpnI G_GTAC'C
Cuts at: 2188 2621 2188
Size: 433 4863
MfeI C'AATT_G
Cuts at: 2895 2895
Size: 5296
MluI A'CGCG_T
Cuts at: 5172 5172
Size: 5296
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4410 4594 1305
Size: 3105 184 2007
Fragments arranged by size:
3105 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3474
Fragments arranged by size:
3474 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2589 3254 3613 3772 518
Size: 648 1423 665 359 159 2042
Fragments arranged by size:
2042 1423 665 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2341 2712 3389 4537 4782 1606
Size: 735 371 677 1148 245 2120
Fragments arranged by size:
2120 1148 735 677 371 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4744
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5296
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5296
NotI GC'GGCC_GC
Cuts at: 2743 2743
Size: 5296
NspI r_CATG'y
Cuts at: 1132 1229 2044 2076 2456 2654 4199 1132
Size: 97 815 32 380 198 1545 2229
Fragments arranged by size:
2229 1545 815 380 198 97 32
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5296
PciI A'CATG_T
Cuts at: 2040 2072 4195 2040
Size: 32 2123 3141
Fragments arranged by size:
3141 2123 32
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2439 2510 1108
Size: 1331 71 3894
Fragments arranged by size:
3894 1331 71
PmeI GTTT'AAAC
Cuts at: 2600 2600
Size: 5296
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3365 3397 4878 4910 3365
Size: 32 1481 32 3751
Fragments arranged by size:
3751 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5296
PsiI TTA'TAA
Cuts at: 2866 2866
Size: 5296
PstI C_TGCA'G
Cuts at: 830 2648 830
Size: 1818 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5296
PvuII CAG'CTG
Cuts at: 2341 2341
Size: 5296
SacI G_AGCT'C
Cuts at: 2113 2113
Size: 5296
SalI G'TCGA_C
Cuts at: 1936 2638 1936
Size: 702 4594
SbfI CC_TGCA'GG
Cuts at: 2648 2648
Size: 5296
ScaI AGT'ACT
Cuts at: 3544 5256 3544
Size: 1712 3584
SfcI C'TryA_G
Cuts at: 826 1045 2644 3779 4460 4651 826
Size: 219 1599 1135 681 191 1471
Fragments arranged by size:
1599 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5296
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5296
SmaI CCC'GGG
Cuts at: 1872 2623 1872
Size: 751 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2423 3400 4301 4563 820
Size: 197 926 480 977 901 262 1553
Fragments arranged by size:
1553 977 926 901 480 262 197
SnaBI TAC'GTA
Cuts at: 493 3087 493
Size: 2594 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5296
SphI G_CATG'C
Cuts at: 1132 2456 2654 1132
Size: 1324 198 3774
Fragments arranged by size:
3774 1324 198
SspI AAT'ATT
Cuts at: 5 52 2118 3220 5
Size: 47 2066 1102 2081
Fragments arranged by size:
2081 2066 1102 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5296
StyI C'CwwG_G
Cuts at: 513 1065 2735 513
Size: 552 1670 3074
Fragments arranged by size:
3074 1670 552
SwaI ATTT'AAAT
Cuts at: 2401 2401
Size: 5296
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3323 3340 3493 3678 4123 325
Size: 1255 1743 17 153 185 445 1498
Fragments arranged by size:
1743 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2524 3542 5254
Size: 274 80 33 51 1443 547 1018 1712
Cuts at: 5254 96
Size: 138
Fragments arranged by size:
1712 1443 1018 547 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5256 5288 5256
Size: 32 5264
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5296
XbaI T'CTAG_A
Cuts at: 2632 2632
Size: 5296
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2292 2292
Size: 5296
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5296
XmaI C'CCGG_G
Cuts at: 1870 2621 1870
Size: 751 4545
XmnI GAAnn'nnTTC
Cuts at: 3425 3425
Size: 5296
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3104 276
Size: 53 83 186 936 1570 2468
Fragments arranged by size:
2468 1570 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI