Restriction map for
pFN21AE3637
(Circular) (Six-base) MAPSORT of: pFN21AE3637.seq Check: 4939 from: 1 to: 6259
pFN21AE3637 6259 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4069 278
Size: 53 83 186 936 2533 2468
Fragments arranged by size:
2533 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3602 1937
Size: 1665 4594
Acc65I G'GTAC_C
Cuts at: 3580 3580
Size: 6259
AclI AA'CG_TT
Cuts at: 1563 4386 4759 1563
Size: 2823 373 3063
Fragments arranged by size:
3063 2823 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3505 4322 5706 5974 1632
Size: 1873 817 1384 268 1917
Fragments arranged by size:
1917 1873 1384 817 268
AfeI AGC'GCT
Cuts at: 2183 2891 2183
Size: 708 5551
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6062
AflIII A'CryG_T
Cuts at: 1203 5158 6135 1203
Size: 3955 977 1327
Fragments arranged by size:
3955 1327 977
AgeI A'CCGG_T
Cuts at: 5090 5090
Size: 6259
AhdI GACnn_n'nnGTC
Cuts at: 2116 4988 2116
Size: 2872 3387
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6259
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6259
AlwNI CAG_nnn'CTG
Cuts at: 2549 4002 5574 2549
Size: 1453 1572 3234
Fragments arranged by size:
3234 1572 1453
ApaI G_GGCC'C
Cuts at: 2017 2428 2556 2747 2017
Size: 411 128 191 5529
Fragments arranged by size:
5529 411 191 128
ApaLI G'TGCA_C
Cuts at: 4316 5472 4316
Size: 1156 5103
ApoI r'AATT_y
Cuts at: 1493 3568 3794 3946 6055 1493
Size: 2075 226 152 2109 1697
Fragments arranged by size:
2109 2075 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4813 160
Size: 872 3781 1606
Fragments arranged by size:
3781 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6259
AvaI C'yCGr_G
Cuts at: 1870 1943 2295 2907 3584 1870
Size: 73 352 612 677 4545
Fragments arranged by size:
4545 677 612 352 73
BamHI G'GATC_C
Cuts at: 2124 3589 3956 2124
Size: 1465 367 4427
Fragments arranged by size:
4427 1465 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2788 3580 5035 618
Size: 325 217 627 1001 792 1455 1842
Fragments arranged by size:
1842 1455 1001 792 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2017 2428 2463 2489 2556 2589 2747 3036
Size: 626 411 35 26 67 33 158 289
Cuts at: 3036 3192 3303 3519 3578 1391
Size: 156 111 216 59 4072
Fragments arranged by size:
4072 626 411 289 216 158 156 111
67 59 35 33 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2069 928
Size: 971 170 5118
Fragments arranged by size:
5118 971 170
BbvCI CC'TCA_GC
Cuts at: 2637 2637
Size: 6259
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2232 2266 4450 4484 1903
Size: 34 295 34 2184 34 3678
Fragments arranged by size:
3678 2184 295 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4151 5361 4151
Size: 1210 5049
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6259
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4870 136
Size: 107 122 71 631 605 3198 1525
Fragments arranged by size:
3198 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3456 6254 3456
Size: 2798 3461
BlpI GC'TnA_GC
Cuts at: 3676 3676
Size: 6259
Bme1580I G_kGCm'C
Cuts at: 1790 2017 2428 2556 2747 2968 4320 5476 1790
Size: 227 411 128 191 221 1352 1156 2573
Fragments arranged by size:
2573 1352 1156 411 227 221 191 128
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6259
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2744 2760 3990
Size: 416 53 83 399 24 1327 16 1230
Cuts at: 3990 4948 442
Size: 958 1753
Fragments arranged by size:
1753 1327 1230 958 416 399 83 53
24 16
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2400 2891 3362 4919 1239
Size: 1161 491 471 1557 2579
Fragments arranged by size:
2579 1557 1161 491 471
Bpu10I CC'TnA_GC
Cuts at: 2622 2637 2622
Size: 15 6244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4384 5249 5547 4384
Size: 865 298 5096
Fragments arranged by size:
5096 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4922 882
Size: 617 3423 2219
Fragments arranged by size:
3423 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2995 4050 493
Size: 711 1791 1055 2702
Fragments arranged by size:
2702 1791 1055 711
BsaBI GATnn'nnATC
Cuts at: 6253 6253
Size: 6259
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4066 4448 5083 275
Size: 53 83 186 936 2533 382 635 1451
Fragments arranged by size:
2533 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2782 4692 5090 5364 5511 5922 2782
Size: 1910 398 274 147 411 3119
Fragments arranged by size:
3119 1910 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2888 2918 3415 3445 1178
Size: 30 165 30 1485 30 497 30 3992
Fragments arranged by size:
3992 1485 497 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2479 1186
Size: 1293 4966
BseYI C'CCAG_C
Cuts at: 2284 5462 5958 2284
Size: 3178 496 2585
Fragments arranged by size:
3178 2585 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2188 2378 2846 1163
Size: 573 452 190 468 4576
Fragments arranged by size:
4576 573 468 452 190
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2233 2317 3709 4470 5498 1540
Size: 275 186 232 84 1392 761 1028 2301
Fragments arranged by size:
2301 1392 1028 761 275 232 186 84
BsiHKAI G_wGCw'C
Cuts at: 2820 3036 3192 3519 4320 4405 5476 2820
Size: 216 156 327 801 85 1071 3603
Fragments arranged by size:
3603 1071 801 327 216 156 85
BsmI GAATG_Cn'
Cuts at: 3770 3863 3770
Size: 93 6166
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2017 2428 2463 2489 2556 2589 2747
Size: 399 227 411 35 26 67 33 158
Cuts at: 2747 2820 2968 3036 3192 3303 3519 3578 4320
Size: 73 148 68 156 111 216 59 742
Cuts at: 4320 4405 5476 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 411 399 227 216 158
156 148 111 85 73 68 67 59
35 33 26
BspEI T'CCGG_A
Cuts at: 5922 5922
Size: 6259
BspHI T'CATG_A
Cuts at: 4148 5878 4148
Size: 1730 4529
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2508 2886 3614 844
Size: 1664 378 728 3489
Fragments arranged by size:
3489 1664 728 378
BsrBI CCG'CTC
Cuts at: 1394 1823 2626 4146 1394
Size: 429 803 1520 3507
Fragments arranged by size:
3507 1520 803 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3081 4754 4928 65
Size: 1183 1833 1673 174 1396
Fragments arranged by size:
1833 1673 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4903 5090 1700
Size: 110 3093 187 2869
Fragments arranged by size:
3093 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6259
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6259
BssSI C'ACGA_G
Cuts at: 4319 5331 4319
Size: 1012 5247
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2795 2824 794
Size: 2001 29 4229
Fragments arranged by size:
4229 2001 29
BstBI TT'CG_AA
Cuts at: 5063 5063
Size: 6259
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6259
BstXI CCAn_nnnn'nTGG
Cuts at: 2572 2572
Size: 6259
BstYI r'GATC_y
Cuts at: 1972 2124 3456 3589 3956 4341 4358 5810 5896
Size: 152 1332 133 367 385 17 1452 86
Cuts at: 5896 5908 6254 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1332 385 367 346 152 133
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2505 2542 2505
Size: 37 6222
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4768
Fragments arranged by size:
4768 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3362 487
Size: 683 56 2136 3384
Fragments arranged by size:
3384 2136 683 56
BtsI GCAGTG_nn'
Cuts at: 2257 3775 4569 4589 2257
Size: 1518 794 20 3927
Fragments arranged by size:
3927 1518 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6259
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2086 2121 3063 3098 3133 3168 529
Size: 35 1522 35 942 35 35 35 3620
Fragments arranged by size:
3620 1522 942 35 35 35 35 35
DraI TTT'AAA
Cuts at: 3563 3563
Size: 6259
DraIII CAC_nnn'GTG
Cuts at: 2824 2824
Size: 6259
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5266 809
Size: 507 3950 1802
Fragments arranged by size:
3950 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2314 2574 3293
Size: 54 1425 50 275 18 484 260 719
Cuts at: 3293 3706 4595 6224 8
Size: 413 889 1629 43
Fragments arranged by size:
1629 1425 889 719 484 413 275 260
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2314 3706 1537
Size: 275 502 1392 4090
Fragments arranged by size:
4090 1392 502 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3524 4189 1353
Size: 2171 665 3423
Fragments arranged by size:
3423 2171 665
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2159 4846 5220 5366 387
Size: 1064 138 570 2687 374 146 1280
Fragments arranged by size:
2687 1280 1064 570 374 146 138
EcoICRI GAG'CTC
Cuts at: 3034 3190 3517 3034
Size: 156 327 5776
Fragments arranged by size:
5776 327 156
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2400 2891 3362 3505 4322 4919 5706
Size: 393 768 491 471 143 817 597 787
Cuts at: 5706 5974 1239
Size: 268 1524
Fragments arranged by size:
1524 817 787 768 597 491 471 393
268 143
EcoNI CCTnn'n_nnAGG
Cuts at: 2418 2504 2978 2418
Size: 86 474 5699
Fragments arranged by size:
5699 474 86
EcoO109I rG'GnC_Cy
Cuts at: 2013 2014 2424 2553 2595 2743 2875 2013
Size: 1 410 129 42 148 132 5397
Fragments arranged by size:
5397 410 148 132 129 42 1
EcoRI G'AATT_C
Cuts at: 3568 3946 3568
Size: 378 5881
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2182 3535 2182
Size: 1353 4906
FspI TGC'GCA
Cuts at: 4765 4765
Size: 6259
HaeII r_GCGC'y
Cuts at: 1678 2185 2285 2893 5406 1678
Size: 507 100 608 2513 2531
Fragments arranged by size:
2531 2513 608 507 100
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2102 2134 2289 2321 4900
Size: 32 135 32 530 32 155 32 2579
Cuts at: 4900 4932 4974 5006 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2579 530 155 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3603 3849 669
Size: 988 281 1665 246 3079
Fragments arranged by size:
3079 1665 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3416 748
Size: 2668 3591
HpaI GTT'AAC
Cuts at: 3849 3849
Size: 6259
KpnI G_GTAC'C
Cuts at: 3584 3584
Size: 6259
MfeI C'AATT_G
Cuts at: 3858 3858
Size: 6259
MluI A'CGCG_T
Cuts at: 6135 6135
Size: 6259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2589 2919 5373 5557 1305
Size: 1284 330 2454 184 2007
Fragments arranged by size:
2454 2007 1284 330 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2576 10
Size: 54 1425 343 744 3693
Fragments arranged by size:
3693 1425 744 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4217 4576 4735 518
Size: 648 3051 359 159 2042
Fragments arranged by size:
3051 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2368 2377 2476 2674 2863 3328 3675 4352
Size: 762 9 99 198 189 465 347 677
Cuts at: 4352 5500 5745 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 762 677 465 347 245 198
189 99 9
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4768
Fragments arranged by size:
4768 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6259
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6259
NotI GC'GGCC_GC
Cuts at: 3706 3706
Size: 6259
NspI r_CATG'y
Cuts at: 1132 1229 2293 2323 2351 2859 3443 3617 5162
Size: 97 1064 30 28 508 584 174 1545
Cuts at: 5162 1132
Size: 2229
Fragments arranged by size:
2229 1545 1064 584 508 174 97 30
28
PasI CC'CwG_GG
Cuts at: 1348 2482 1348
Size: 1134 5125
PciI A'CATG_T
Cuts at: 5158 5158
Size: 6259
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3442 1108
Size: 2334 3925
PmeI GTTT'AAAC
Cuts at: 3563 3563
Size: 6259
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4328 4360 5841 5873 4328
Size: 32 1481 32 4714
Fragments arranged by size:
4714 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2875 2875
Size: 6259
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6259
PsiI TTA'TAA
Cuts at: 3829 3829
Size: 6259
PspOMI G'GGCC_C
Cuts at: 2013 2424 2552 2743 2013
Size: 411 128 191 5529
Fragments arranged by size:
5529 411 191 128
PstI C_TGCA'G
Cuts at: 830 2271 2373 3185 3333 3456 3611 830
Size: 1441 102 812 148 123 155 3478
Fragments arranged by size:
3478 1441 812 155 148 123 102
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6259
PvuII CAG'CTG
Cuts at: 2368 2476 2674 2863 3328 2368
Size: 108 198 189 465 5299
Fragments arranged by size:
5299 465 198 189 108
SacI G_AGCT'C
Cuts at: 3036 3192 3519 3036
Size: 156 327 5776
Fragments arranged by size:
5776 327 156
SalI G'TCGA_C
Cuts at: 1936 3601 1936
Size: 1665 4594
SapI GCTCTTCn'nnn_
Cuts at: 3524 3524
Size: 6259
SbfI CC_TGCA'GG
Cuts at: 3611 3611
Size: 6259
ScaI AGT'ACT
Cuts at: 4507 6219 4507
Size: 1712 4547
SexAI A'CCwGG_T
Cuts at: 2820 2820
Size: 6259
SfcI C'TryA_G
Cuts at: 826 1045 2267 2369 2656 2845 3181 3329 3452
Size: 219 1222 102 287 189 336 148 123
Cuts at: 3452 3607 4742 5423 5614 826
Size: 155 1135 681 191 1471
Fragments arranged by size:
1471 1222 1135 681 336 287 219 191
189 155 148 123 102
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6259
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6259
SmaI CCC'GGG
Cuts at: 1872 2909 3586 1872
Size: 1037 677 4545
Fragments arranged by size:
4545 1037 677
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4363 5264 5526 820
Size: 197 926 2420 901 262 1553
Fragments arranged by size:
2420 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4050 493
Size: 3557 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6259
SphI G_CATG'C
Cuts at: 1132 2351 3617 1132
Size: 1219 1266 3774
Fragments arranged by size:
3774 1266 1219
SspI AAT'ATT
Cuts at: 5 52 4183 5
Size: 47 4131 2081
Fragments arranged by size:
4131 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6259
StyI C'CwwG_G
Cuts at: 513 1065 2004 2428 2454 2577 3174 3408 3555
Size: 552 939 424 26 123 597 234 147
Cuts at: 3555 3698 513
Size: 143 3074
Fragments arranged by size:
3074 939 597 552 424 234 147 143
123 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2700 3421 4286 4303 4456 4641 5086
Size: 1255 1120 721 865 17 153 185 445
Cuts at: 5086 325
Size: 1498
Fragments arranged by size:
1498 1255 1120 865 721 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3333 4505 6217
Size: 274 80 33 51 1443 1356 1172 1712
Cuts at: 6217 96
Size: 138
Fragments arranged by size:
1712 1443 1356 1172 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6219 6251 6219
Size: 32 6227
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6259
XbaI T'CTAG_A
Cuts at: 3595 3595
Size: 6259
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2992 2992
Size: 6259
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6259
XmaI C'CCGG_G
Cuts at: 1870 2907 3584 1870
Size: 1037 677 4545
Fragments arranged by size:
4545 1037 677
XmnI GAAnn'nnTTC
Cuts at: 4388 4388
Size: 6259
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4067 276
Size: 53 83 186 936 2533 2468
Fragments arranged by size:
2533 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI